Rorug02G0238000

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011127
Physical Location & Seq
Reverse (-)
23863005 .. 23864144
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0238000.1

Sequence Viewer

Length: 1140 bp
ATGATGCAAGTCTACGGAAACCAGAGAGGTCCTTATGGTCCAATGAAGAATTCTATGGTTCCTGTCAAGAATCAAGATCCATGTAAGCCACGCTATATGCCTTTTCCGGCTAATCACCGGAACCAGGTGCCGCCGGTCGGTCATTTTCAGCCCCAATTTCTTCCACCTCGACCAAATTTCTACCCATTCCCACAAACCCTCGTGCCACCTTATTCATCTGGTGGGTACAATTTTCAGGGGCAGGGTGATTCTACTTCAGCAAGTTCTGTCCCTCATCGTCTACCATCACAATGCGTAACTCCGGTTAACAGTGCTGGTCCAGTAGTCTCCGGTTTGCTTGGGTCTCTAATGGCACAAGGTGTGATCTCATTGAAAAATCAAAGCAGAGGAGGACTGGAGGGTACTAGTTCACTAAATTCTGTACCTCTTGTATCACAGCGAGGCGCATTTCCTGTTCAAAATGCTGCTGTTGATGTTCCAGTAGTAGTTCCTGGTTTGATTGATGCTCTCCTGGCACAAGGTGTGATTTCATTGAAGAAACAAAGCCCGGCACTGAAAGAGGAGAAGGATGCTGTGGTAGGATGCGAGTTTTCCGGCCTTGATCTAAATCTGCATCGCGAATCTGTGGTAATAGGCTTGTATGAGGGTGGTCTGCCAAGGCAATGTAAAAGCTGTGGCCTCCGGTTCAAGTGTGAAGAACAGCACAGCAGCCATATGGATTGGCATGTCAAGAAGAACCGGATGTCCAAAACCCGCAAGCAGAAGCCCTCTCGTAACTTTTATGCAACTACAAGCATGTGGCTTGTTACTGATGTAGAGGCTGAGGCTGAGGCTGAGGCATTACCTTCTGGTGTAGTTTTGAGCGCTGCTGACACCAGTGGCGTGGCGGAAAAGAAAAGGAAGACTAGTGATGAAGAAATGGTTGTTCCTGCGGACGAGGATCAGAGTAAATGTGCCTTATGCACAGATGCTTTTGTTGAGTTTTATAGTCATGAGAAAGATGAGTGGATGTATAAAGGTGCTGTTTACTTGAATGCGCCTCAGGGGCCAGCGCCGGAGATGGATCGGTCACAGTTGGGTCCTATAGTCCATGCCAAATGCTGGTCGGAGGGTAGTAGGAAACGTCTGCGGAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

379

Amino Acids

41.46

Weight (kDa)

8.75

Isoelectric Point (pI)

54.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_PCFS4 PF23228 304 - 370 4.2e-28 PCFS4 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011127)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G39450
fragaria_vesca FvH4_1g24840 FvH4_1g24840 FvH4_1g24840
malus_domestica MD15G1134900.v1.1
prunus_persica Prupe.4G275200_v2.0.a1 Prupe.4G275200_v2.0.a1
pyrus_communis pycom15g12170
rosa_chinensis RchiOBHm_Chr2g0122451
rosa_laevigata RLG00000018678
rosa_multiflora Rmu_co8054258.1_g000001 Rmu_sc0003832.1_g000018
rosa_roxburghii Rroxscaffold_2G00121630
rosa_rugosa Rorug02G0238000 Rorug02G0238100
rosa_samantha Rh2AG297400 Rh2BG306200 Rh2CG284500 Rh2DG321000
rosa_wichuraiana Rw2G023660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 127
AccB7I CCANNNNNTGG 1 cut(s) 1101
AccI GTMKAC 2 cut(s) 12, 280
AccII CGCG 1 cut(s) 618
AciI CCGC 5 cut(s) 131, 754, 887, 932, 1129
AclWI GGATC 3 cut(s) 71, 948, 1071
AcsI RAATTY 3 cut(s) 49, 175, 415
AcuI CTGAAG 1 cut(s) 240
AdeI CACNNNGTG 2 cut(s) 359, 521
AfaI GTAC 3 cut(s) 227, 403, 423
AfeI AGCGCT 1 cut(s) 865
AfiI CCNNNNNNNGG 3 cut(s) 124, 137, 1101
AgsI TTSAA 5 cut(s) 373, 458, 535, 688, 1033
AhlI ACTAGT 2 cut(s) 404, 905
AjnI CCWGG 3 cut(s) 123, 490, 510
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AloI GAACNNNNNNTCC 2 cut(s) 728, 760
AluBI AGCT 1 cut(s) 672
AluI AGCT 1 cut(s) 672
Alw26I GTCTC 2 cut(s) 331, 348
AlwI GGATC 3 cut(s) 71, 948, 1071
Aor51HI AGCGCT 1 cut(s) 865
AoxI GGCC 3 cut(s) 595, 676, 1046
ApeKI GCWGC 3 cut(s) 464, 708, 866
ApoI RAATTY 3 cut(s) 49, 175, 415
AspLEI GCGC 4 cut(s) 446, 866, 1039, 1054
AspS9I GGNCC 5 cut(s) 29, 38, 317, 1046, 1079
AsuC2I CCSGG 1 cut(s) 548
AsuHPI GGTGA 2 cut(s) 107, 257
AvaII GGWCC 4 cut(s) 29, 38, 317, 1079
AxyI CCTNAGG 1 cut(s) 1041
BanI GGYRCC 1 cut(s) 127
BauI CACGAG 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 908
BbvCI CCTCAGC 3 cut(s) 822, 828, 834
BbvI GCAGC 3 cut(s) 451, 720, 853
BccI CCATC 2 cut(s) 292, 1054
BciT130I CCWGG 3 cut(s) 125, 492, 512
BcnI CCSGG 1 cut(s) 548
BcoDI GTCTC 2 cut(s) 331, 348
BcuI ACTAGT 2 cut(s) 404, 905
BfaI CTAG 2 cut(s) 405, 906
BfmI CTRYAG 1 cut(s) 1083
BfoI RGCGCY 2 cut(s) 867, 1055
BglI GCCNNNNNGGC 1 cut(s) 1045
BisI GCNGC 4 cut(s) 131, 465, 709, 867
BlsI GCNGC 4 cut(s) 132, 466, 710, 868
Bme1390I CCNGG 4 cut(s) 125, 492, 512, 548
Bme18I GGWCC 4 cut(s) 29, 38, 317, 1079
BmgT120I GGNCC 5 cut(s) 29, 38, 317, 1046, 1079
BmiI GGNNCC 5 cut(s) 60, 122, 129, 1047, 1080
BmrFI CCNGG 4 cut(s) 125, 492, 512, 548
BmsI GCATC 5 cut(s) 493, 559, 572, 622, 958
BpiI GAAGAC 1 cut(s) 908
BpmI CTGGAG 1 cut(s) 416
Bpu10I CCTNAGC 3 cut(s) 822, 828, 834
BpuMI CCSGG 1 cut(s) 548
BsaBI GATNNNNATC 1 cut(s) 606
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 656
BsaWI WCCGGW 5 cut(s) 117, 301, 329, 681, 738
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 137, 1101
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 4 cut(s) 320, 399, 479, 876
Bse21I CCTNAGG 1 cut(s) 1041
Bse3DI GCAATG 1 cut(s) 668
Bse8I GATNNNNATC 1 cut(s) 606
BseBI CCWGG 3 cut(s) 125, 492, 512
BseDI CCNNGG 1 cut(s) 656
BseGI GGATG 4 cut(s) 574, 587, 747, 1014
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 3 cut(s) 124, 137, 1101
BseMI GCAATG 1 cut(s) 668
BseMII CTCAG 4 cut(s) 813, 819, 825, 1055
BseNI ACTGG 4 cut(s) 320, 399, 479, 876
BseRI GAGGAG 2 cut(s) 402, 575
BseXI GCAGC 3 cut(s) 451, 720, 853
Bsh1236I CGCG 1 cut(s) 618
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 3 cut(s) 597, 678, 1048
BshNI GGYRCC 1 cut(s) 127
BsiEI CGRYCG 1 cut(s) 138
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 3 cut(s) 124, 137, 1101
BsmAI GTCTC 2 cut(s) 331, 348
BsmFI GGGAC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 1039
BsnI GGCC 3 cut(s) 597, 678, 1048
Bso31I GGTCTC 1 cut(s) 348
Bsp143I GATC 5 cut(s) 76, 363, 601, 940, 1063
Bsp68I TCGCGA 1 cut(s) 618
BspACI CCGC 5 cut(s) 131, 754, 887, 932, 1129
BspANI GGCC 3 cut(s) 597, 678, 1048
BspCNI CTCAG 4 cut(s) 814, 820, 826, 1054
BspFNI CGCG 1 cut(s) 618
BspHI TCATGA 1 cut(s) 991
BspLI GGNNCC 5 cut(s) 60, 122, 129, 1047, 1080
BspPI GGATC 3 cut(s) 71, 948, 1071
BspT107I GGYRCC 1 cut(s) 127
BspTNI GGTCTC 1 cut(s) 348
BsrDI GCAATG 1 cut(s) 668
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 4 cut(s) 320, 399, 479, 876
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 656
BssMI GATC 5 cut(s) 76, 363, 601, 940, 1063
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 656
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 3 cut(s) 125, 492, 512
Bst4CI ACNGT 2 cut(s) 311, 1074
BstC8I GCNNGC 2 cut(s) 758, 1050
BstDEI CTNAG 4 cut(s) 822, 828, 834, 1041
BstF5I GGATG 4 cut(s) 574, 587, 747, 1014
BstFNI CGCG 1 cut(s) 618
BstH2I RGCGCY 2 cut(s) 867, 1055
BstHHI GCGC 4 cut(s) 446, 866, 1039, 1054
BstKTI GATC 5 cut(s) 79, 366, 604, 943, 1066
BstMAI GTCTC 2 cut(s) 331, 348
BstMBI GATC 5 cut(s) 76, 363, 601, 940, 1063
BstMCI CGRYCG 1 cut(s) 138
BstMWI GCNNNNNNNGC 2 cut(s) 512, 1045
BstNI CCWGG 3 cut(s) 125, 492, 512
BstNSI RCATGY 2 cut(s) 728, 799
BstSCI CCNGG 4 cut(s) 123, 490, 510, 546
BstSFI CTRYAG 1 cut(s) 1083
BstUI CGCG 1 cut(s) 618
BstV1I GCAGC 3 cut(s) 451, 720, 853
BstV2I GAAGAC 1 cut(s) 908
BstX2I RGATCY 1 cut(s) 76
BstXI CCANNNNNNTGG 1 cut(s) 883
BstYI RGATCY 1 cut(s) 76
Bsu36I CCTNAGG 1 cut(s) 1041
BsuRI GGCC 3 cut(s) 597, 678, 1048
BtgZI GCGATG 1 cut(s) 599
BtsCI GGATG 4 cut(s) 574, 587, 747, 1014
BtsIMutI CAGTG 3 cut(s) 316, 551, 883
BtuMI TCGCGA 1 cut(s) 618
Cac8I GCNNGC 2 cut(s) 758, 1050
CciI TCATGA 1 cut(s) 991
CfoI GCGC 4 cut(s) 446, 866, 1039, 1054
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 5 cut(s) 29, 38, 317, 1046, 1079
CsiI ACCWGGT 1 cut(s) 123
Csp6I GTAC 3 cut(s) 226, 402, 422
CviAII CATG 5 cut(s) 81, 725, 796, 992, 1091
CviQI GTAC 3 cut(s) 226, 402, 422
DdeI CTNAG 4 cut(s) 822, 828, 834, 1041
DpnI GATC 5 cut(s) 78, 365, 603, 942, 1065
DpnII GATC 5 cut(s) 76, 363, 601, 940, 1063
DraIII CACNNNGTG 2 cut(s) 359, 521
EciI GGCGGA 1 cut(s) 902
Eco130I CCWWGG 1 cut(s) 656
Eco31I GGTCTC 1 cut(s) 348
Eco47I GGWCC 4 cut(s) 29, 38, 317, 1079
Eco47III AGCGCT 1 cut(s) 865
Eco57I CTGAAG 1 cut(s) 240
Eco81I CCTNAGG 1 cut(s) 1041
EcoO109I RGGNCCY 2 cut(s) 29, 1079
EcoRI GAATTC 1 cut(s) 49
EcoRII CCWGG 3 cut(s) 123, 490, 510
EcoT14I CCWWGG 1 cut(s) 656
ErhI CCWWGG 1 cut(s) 656
FaeI CATG 5 cut(s) 84, 728, 799, 995, 1094
FaqI GGGAC 1 cut(s) 254
FatI CATG 5 cut(s) 80, 724, 795, 991, 1090
FauI CCCGC 1 cut(s) 761
FauNDI CATATG 1 cut(s) 714
FblI GTMKAC 2 cut(s) 12, 280
Fnu4HI GCNGC 4 cut(s) 131, 465, 709, 867
FokI GGATG 4 cut(s) 581, 594, 754, 1021
Fsp4HI GCNGC 4 cut(s) 131, 465, 709, 867
FspBI CTAG 2 cut(s) 405, 906
GlaI GCGC 4 cut(s) 445, 865, 1038, 1053
GluI GCNGC 4 cut(s) 131, 465, 709, 867
GsuI CTGGAG 1 cut(s) 416
HaeII RGCGCY 2 cut(s) 867, 1055
HaeIII GGCC 3 cut(s) 597, 678, 1048
HhaI GCGC 4 cut(s) 446, 866, 1039, 1054
Hin1II CATG 5 cut(s) 84, 728, 799, 995, 1094
Hin6I GCGC 4 cut(s) 444, 864, 1037, 1052
HinP1I GCGC 4 cut(s) 444, 864, 1037, 1052
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 3 cut(s) 70, 248, 620
HpaI GTTAAC 1 cut(s) 307
HphI GGTGA 2 cut(s) 107, 257
Hpy166II GTNNAC 5 cut(s) 13, 281, 307, 410, 1027
Hpy188I TCNGA 2 cut(s) 945, 1108
Hpy188III TCNNGA 5 cut(s) 67, 74, 617, 730, 992
Hpy8I GTNNAC 5 cut(s) 13, 281, 307, 410, 1027
HpyAV CCTTC 2 cut(s) 559, 855
HpyCH4III ACNGT 2 cut(s) 311, 1074
HpyCH4IV ACGT 1 cut(s) 1123
HpyCH4V TGCA 4 cut(s) 7, 613, 785, 963
HpyF10VI GCNNNNNNNGC 2 cut(s) 512, 1045
HpyF3I CTNAG 4 cut(s) 822, 828, 834, 1041
HpySE526I ACGT 1 cut(s) 1123
Hsp92II CATG 5 cut(s) 84, 728, 799, 995, 1094
HspAI GCGC 4 cut(s) 444, 864, 1037, 1052
KspAI GTTAAC 1 cut(s) 307
Kzo9I GATC 5 cut(s) 76, 363, 601, 940, 1063
Lsp1109I GCAGC 3 cut(s) 451, 720, 853
LweI GCATC 5 cut(s) 493, 559, 572, 622, 958
MabI ACCWGGT 1 cut(s) 123
MaeI CTAG 2 cut(s) 405, 906
MaeII ACGT 1 cut(s) 1123
MaeIII GTNAC 4 cut(s) 295, 773, 805, 1068
MalI GATC 5 cut(s) 78, 365, 603, 942, 1065
MboI GATC 5 cut(s) 76, 363, 601, 940, 1063
MboII GAAGA 7 cut(s) 58, 152, 547, 707, 745, 913, 926
MflI RGATCY 1 cut(s) 76
MluCI AATT 5 cut(s) 49, 155, 175, 229, 415
MmeI TCCRAC 1 cut(s) 1086
MseI TTAA 1 cut(s) 306
MslI CAYNNNNRTG 1 cut(s) 289
MspR9I CCNGG 4 cut(s) 125, 492, 512, 548
Mva1269I GAATGC 1 cut(s) 1039
MvaI CCWGG 3 cut(s) 125, 492, 512
MvnI CGCG 1 cut(s) 618
MwoI GCNNNNNNNGC 2 cut(s) 512, 1045
NciI CCSGG 1 cut(s) 548
NdeI CATATG 1 cut(s) 714
NdeII GATC 5 cut(s) 76, 363, 601, 940, 1063
NlaIII CATG 5 cut(s) 84, 728, 799, 995, 1094
NlaIV GGNNCC 5 cut(s) 60, 122, 129, 1047, 1080
NmuCI GTSAC 1 cut(s) 1068
NruI TCGCGA 1 cut(s) 618
NspI RCATGY 2 cut(s) 728, 799
PagI TCATGA 1 cut(s) 991
PctI GAATGC 1 cut(s) 1039
PfeI GAWTC 3 cut(s) 70, 248, 620
PflMI CCANNNNNTGG 1 cut(s) 1101
PkrI GCNGC 4 cut(s) 132, 466, 710, 868
PpuMI RGGWCCY 2 cut(s) 29, 1079
Psp5II RGGWCCY 2 cut(s) 29, 1079
Psp6I CCWGG 3 cut(s) 123, 490, 510
PspGI CCWGG 3 cut(s) 123, 490, 510
PspN4I GGNNCC 5 cut(s) 60, 122, 129, 1047, 1080
PspPI GGNCC 5 cut(s) 29, 38, 317, 1046, 1079
PspPPI RGGWCCY 2 cut(s) 29, 1079
PsuI RGATCY 1 cut(s) 76
RruI TCGCGA 1 cut(s) 618
RsaI GTAC 3 cut(s) 227, 403, 423
RsaNI GTAC 3 cut(s) 226, 402, 422
RseI CAYNNNNRTG 1 cut(s) 289
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 4 cut(s) 131, 465, 709, 867
Sau3AI GATC 5 cut(s) 76, 363, 601, 940, 1063
Sau96I GGNCC 5 cut(s) 29, 38, 317, 1046, 1079
ScrFI CCNGG 4 cut(s) 125, 492, 512, 548
SexAI ACCWGGT 1 cut(s) 123
SfaNI GCATC 5 cut(s) 493, 559, 572, 622, 958
SfcI CTRYAG 1 cut(s) 1083
SinI GGWCC 4 cut(s) 29, 38, 317, 1079
SmiMI CAYNNNNRTG 1 cut(s) 289
SpeI ACTAGT 2 cut(s) 404, 905
Sse9I AATT 5 cut(s) 49, 155, 175, 229, 415
SsiI CCGC 5 cut(s) 131, 754, 887, 932, 1129
SspMI CTAG 2 cut(s) 405, 906
StyD4I CCNGG 4 cut(s) 123, 490, 510, 546
StyI CCWWGG 1 cut(s) 656
TaaI ACNGT 2 cut(s) 311, 1074
TaiI ACGT 1 cut(s) 1126
TaqI TCGA 1 cut(s) 169
TaqII GACCGA 2 cut(s) 128, 1056
TasI AATT 5 cut(s) 49, 155, 175, 229, 415
TauI GCSGC 1 cut(s) 133
TfiI GAWTC 3 cut(s) 70, 248, 620
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 3 cut(s) 316, 558, 883
TseFI GTSAC 1 cut(s) 1068
TseI GCWGC 3 cut(s) 464, 708, 866
Tsp45I GTSAC 1 cut(s) 1068
TspDTI ATGAA 4 cut(s) 59, 204, 519, 927
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 3 cut(s) 316, 558, 883
Van91I CCANNNNNTGG 1 cut(s) 1101
VpaK11BI GGWCC 4 cut(s) 29, 38, 317, 1079
XapI RAATTY 3 cut(s) 49, 175, 415
XceI RCATGY 2 cut(s) 728, 799
XmiI GTMKAC 2 cut(s) 12, 280
XspI CTAG 2 cut(s) 405, 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.