pycom15g12910

Inositol-tetrakisphosphate 1-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009330
Physical Location & Seq
Forward (+)
8829669 .. 8833520
3852 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1515 bp
ATGGCAGGCGTTGGCGGTGGCGGTGTAGGAGGAGTGATATTGGACGAGTGTGTACTTTTTGGTGGTACCAATGTCAACTTGCAATCAGACGCCCACTTTCTGCTTCATAAGCTTCGCCATGCCAACATTCCTACGGGAATTTCTTATGGAGCAGGTCTTGAAGCTCATAAGGTTAATATTCTCAAAGAAATGGCGGCACAGTATTCCATCCATTGCTTCCTCTTAGATGAATCCGCATCCTCTACAGACGATGCTATACGTGAGGTTATGCTAGCTTGGCCTAATACCCGAGGCTGTATTTTGTATATAGTATCCAACAACAAGGAATACATGTTTCCTAAACTAAGCAAATGCGGTTGGCTTATCACGCTTCTGAATGTTGAAGGCAGCCCCGCGTCCACATGTGAGAGTTCAAGCATGGTGTATATCAACAAACTACGCGAGTTGCCTTTGACTATTTGCCAAATAAATAGAAAGGCATTTGGAAACAGCATTGTGACAGTTGGTTATATTATGAAGCCGTCTCGTGAAGGGGATTTCGCTAAGAGGGGTGCATTCCCTATGTATCCTACTCAAGATGGGTTGATGTTCGTTCCCCTCACATTTGATCTCCCTCTAGCACCTCAATTACAAGAAGTTGATGTAGTTCTCCATAAAGCAACCGATGAAATTATATCCATTAATTTGGATGGATCTTTACCATCTTCTACCAGCATAACATACAGCAGGGGAATGCAGGAATTGCAAAGATATATGGAACATCACCTAGACCTCTGTGTGATTGACCCACTTAGCTCTATCTATCCTGTAGTAGATCGATTGAAAATTCAACAGATTCTACTTGGGTTGGAGGATCTCAAAACCGCCGGTTGCTGCGCAATTAGGGGGCCTAATTTTCTTAAGGTAGATGATTTTAATCAGTCTGATTTGGTTCAAAGCCTATCTGAAGCTAAGTTAGCTCTTCCAAGTATAGTAAAACCTCAAGTTGCTTGTGGTGTGGCTGATGCTCACAGCATGGCAATAGTTTTTAGAGTTGAAGATTTCAAGGATTTGACTGTTCCTCTTCCAGCAATTATACAGGAATATGTGGATCATTCATCAACGCTCTACAAATTTTACGTGTTGGGTGAGAAAGTTTATCATGCAGTTAAGAGCTCTACTCCAAATGCAGATGGTTTGAAGAAATTATCTGGAAGTAAGGAGCTCAAACCTTTAGTCTTTGACAGCTTAAAATCCTTACCCACTGCCAAAGGGAACCTGAGTTCTGGAGTTGGTAATAGCTCAAAGGCCACTATTGATCTTGAGTTGGTTACTAGTGCAGCTAATTGGCTTATGAGAAATCTTGAGCTGACCATCTTCGGCTTTGATGTTGTTATTGAGGAAGGCACCGGCGACCATGTCATTGTCGATGTAAATTACCTCCCATCATTCAAGGAAGTTCCCAACGAAGTTGCTATCCCTGCGTTTTGGGGTGCGATTAAGAAGAAGTTTGAATTAAGAGGAAGAAATAAGTAG

Protein Analysis

505

Amino Acids

55.17

Weight (kDa)

5.88

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ins134_P3_kin PF05770 296 - 482 2.7e-26 Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 877
Acc36I ACCTGC 1 cut(s) 143
Acc65I GGTACC 1 cut(s) 65
AccB1I GGYRCC 2 cut(s) 65, 1385
AccII CGCG 2 cut(s) 395, 441
AciI CCGC 7 cut(s) 15, 21, 194, 234, 354, 393, 864
AclWI GGATC 3 cut(s) 700, 861, 1098
AcsI RAATTY 3 cut(s) 138, 825, 1112
AcuI CTGAAG 1 cut(s) 966
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 2 cut(s) 54, 67
AflII CTTAAG 1 cut(s) 899
AflIII ACRYGT 3 cut(s) 330, 401, 1119
AhlI ACTAGT 1 cut(s) 1313
AloI GAACNNNNNNTCC 2 cut(s) 1246, 1278
Alw21I GWGCWC 2 cut(s) 1157, 1206
Alw26I GTCTC 1 cut(s) 528
AlwI GGATC 3 cut(s) 700, 861, 1098
Ama87I CYCGRG 1 cut(s) 288
AoxI GGCC 3 cut(s) 278, 887, 1287
ApeKI GCWGC 3 cut(s) 387, 873, 1319
ApoI RAATTY 3 cut(s) 138, 825, 1112
ArsI GACNNNNNNTTYG 2 cut(s) 1200, 1232
AseI ATTAAT 1 cut(s) 681
Asp718I GGTACC 1 cut(s) 65
AspLEI GCGC 1 cut(s) 878
AspS9I GGNCC 1 cut(s) 887
AsuHPI GGTGA 2 cut(s) 755, 1139
AsuNHI GCTAGC 1 cut(s) 271
AvaI CYCGRG 1 cut(s) 288
BaeI ACNNNNGTAYC 2 cut(s) 57, 90
BanI GGYRCC 2 cut(s) 65, 1385
BanII GRGCYC 2 cut(s) 1157, 1206
BauI CACGAG 1 cut(s) 525
Bbv12I GWGCWC 2 cut(s) 1157, 1206
BbvI GCAGC 3 cut(s) 399, 860, 1331
BccI CCATC 7 cut(s) 215, 572, 683, 709, 1166, 1361, 1432
BceAI ACGGC 1 cut(s) 505
BciVI GTATCC 2 cut(s) 322, 576
BcoDI GTCTC 1 cut(s) 528
BcuI ACTAGT 1 cut(s) 1313
BfaI CTAG 4 cut(s) 272, 617, 767, 1314
BfmI CTRYAG 2 cut(s) 243, 807
BfrI CTTAAG 1 cut(s) 899
BfuAI ACCTGC 1 cut(s) 143
BfuI GTATCC 2 cut(s) 322, 576
BisI GCNGC 4 cut(s) 195, 388, 874, 1320
BlsI GCNGC 4 cut(s) 196, 389, 875, 1321
BmeT110I CYCGRG 1 cut(s) 288
BmgT120I GGNCC 1 cut(s) 887
BmiI GGNNCC 4 cut(s) 67, 888, 1256, 1387
BmsI GCATC 3 cut(s) 241, 245, 994
BmtI GCTAGC 1 cut(s) 275
BplI GAGNNNNNCTC 2 cut(s) 1144, 1176
BpmI CTGGAG 1 cut(s) 1287
BpuEI CTTGAG 4 cut(s) 558, 966, 1322, 1364
Bsa29I ATCGAT 1 cut(s) 817
BsaAI YACGTR 2 cut(s) 260, 1120
BsaBI GATNNNNATC 1 cut(s) 915
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 289
Bse118I RCCGGY 2 cut(s) 866, 1388
Bse3DI GCAATG 1 cut(s) 211
Bse8I GATNNNNATC 1 cut(s) 915
BseCI ATCGAT 1 cut(s) 817
BseDI CCNNGG 1 cut(s) 289
BseGI GGATG 3 cut(s) 207, 236, 694
BseJI GATNNNNATC 1 cut(s) 915
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 1 cut(s) 1250
BseRI GAGGAG 1 cut(s) 45
BseXI GCAGC 3 cut(s) 399, 860, 1331
BsgI GTGCAG 1 cut(s) 1338
Bsh1236I CGCG 2 cut(s) 395, 441
BshFI GGCC 3 cut(s) 280, 889, 1289
BshNI GGYRCC 2 cut(s) 65, 1385
BshVI ATCGAT 1 cut(s) 817
BsiHKAI GWGCWC 2 cut(s) 1157, 1206
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 2 cut(s) 867, 1389
BsmAI GTCTC 1 cut(s) 528
BsmBI CGTCTC 1 cut(s) 528
BsmI GAATGC 2 cut(s) 554, 738
BsnI GGCC 3 cut(s) 280, 889, 1289
BsoBI CYCGRG 1 cut(s) 288
Bsp1286I GDGCHC 2 cut(s) 1157, 1206
Bsp143I GATC 6 cut(s) 607, 692, 814, 853, 1090, 1297
BspACI CCGC 7 cut(s) 15, 21, 194, 234, 354, 393, 864
BspANI GGCC 3 cut(s) 280, 889, 1289
BspCNI CTCAG 1 cut(s) 1251
BspDI ATCGAT 1 cut(s) 817
BspFNI CGCG 2 cut(s) 395, 441
BspLI GGNNCC 4 cut(s) 67, 888, 1256, 1387
BspMI ACCTGC 1 cut(s) 143
BspOI GCTAGC 1 cut(s) 275
BspPI GGATC 3 cut(s) 700, 861, 1098
BspQI GCTCTTC 1 cut(s) 966
BspT107I GGYRCC 2 cut(s) 65, 1385
BspTI CTTAAG 1 cut(s) 899
BsrDI GCAATG 1 cut(s) 211
BsrFI RCCGGY 2 cut(s) 866, 1388
BssAI RCCGGY 2 cut(s) 866, 1388
BssECI CCNNGG 1 cut(s) 289
BssMI GATC 6 cut(s) 607, 692, 814, 853, 1090, 1297
BssNI GRCGYC 1 cut(s) 90
BssSI CACGAG 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 525
Bst4CI ACNGT 3 cut(s) 201, 502, 1057
Bst6I CTCTTC 2 cut(s) 966, 1068
BstACI GRCGYC 1 cut(s) 90
BstAFI CTTAAG 1 cut(s) 899
BstAPI GCANNNNNTGC 1 cut(s) 742
BstBAI YACGTR 2 cut(s) 260, 1120
BstC8I GCNNGC 2 cut(s) 7, 273
BstDEI CTNAG 6 cut(s) 223, 344, 543, 791, 951, 1259
BstF5I GGATG 3 cut(s) 207, 236, 694
BstFNI CGCG 2 cut(s) 395, 441
BstHHI GCGC 1 cut(s) 878
BstKTI GATC 6 cut(s) 610, 695, 817, 856, 1093, 1300
BstMAI GTCTC 1 cut(s) 528
BstMBI GATC 6 cut(s) 607, 692, 814, 853, 1090, 1297
BstMWI GCNNNNNNNGC 6 cut(s) 109, 277, 367, 742, 956, 1460
BstNSI RCATGY 2 cut(s) 334, 405
BstSFI CTRYAG 2 cut(s) 243, 807
BstUI CGCG 2 cut(s) 395, 441
BstV1I GCAGC 3 cut(s) 399, 860, 1331
BstX2I RGATCY 2 cut(s) 692, 853
BstXI CCANNNNNNTGG 1 cut(s) 685
BstYI RGATCY 2 cut(s) 692, 853
Bsu15I ATCGAT 1 cut(s) 817
BsuI GTATCC 2 cut(s) 322, 576
BsuRI GGCC 3 cut(s) 280, 889, 1289
BsuTUI ATCGAT 1 cut(s) 817
BtsCI GGATG 3 cut(s) 207, 236, 694
BtsI GCAGTG 1 cut(s) 1242
BtsIMutI CAGTG 1 cut(s) 1242
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 7, 273
CfoI GCGC 1 cut(s) 878
Cfr10I RCCGGY 2 cut(s) 866, 1388
Cfr13I GGNCC 1 cut(s) 887
ClaI ATCGAT 1 cut(s) 817
CseI GACGC 2 cut(s) 98, 384
Csp6I GTAC 2 cut(s) 53, 66
CviAII CATG 7 cut(s) 119, 331, 402, 418, 1015, 1142, 1397
CviQI GTAC 2 cut(s) 53, 66
DdeI CTNAG 6 cut(s) 223, 344, 543, 791, 951, 1259
DpnI GATC 6 cut(s) 609, 694, 816, 855, 1092, 1299
DpnII GATC 6 cut(s) 607, 692, 814, 853, 1090, 1297
Eam1104I CTCTTC 2 cut(s) 966, 1068
EarI CTCTTC 2 cut(s) 966, 1068
Ecl136II GAGCTC 2 cut(s) 1155, 1204
Eco24I GRGCYC 2 cut(s) 1157, 1206
Eco53kI GAGCTC 2 cut(s) 1155, 1204
Eco57I CTGAAG 1 cut(s) 966
Eco88I CYCGRG 1 cut(s) 288
EcoICRI GAGCTC 2 cut(s) 1155, 1204
EcoO109I RGGNCCY 1 cut(s) 887
EcoT38I GRGCYC 2 cut(s) 1157, 1206
Esp3I CGTCTC 1 cut(s) 528
FaeI CATG 7 cut(s) 122, 334, 405, 421, 1018, 1145, 1400
FatI CATG 7 cut(s) 118, 330, 401, 417, 1014, 1141, 1396
FauI CCCGC 1 cut(s) 400
Fnu4HI GCNGC 4 cut(s) 195, 388, 874, 1320
FokI GGATG 3 cut(s) 194, 223, 701
FriOI GRGCYC 2 cut(s) 1157, 1206
Fsp4HI GCNGC 4 cut(s) 195, 388, 874, 1320
FspBI CTAG 4 cut(s) 272, 617, 767, 1314
FspI TGCGCA 1 cut(s) 877
GlaI GCGC 1 cut(s) 877
GluI GCNGC 4 cut(s) 195, 388, 874, 1320
GsuI CTGGAG 1 cut(s) 1287
HaeIII GGCC 3 cut(s) 280, 889, 1289
HapII CCGG 2 cut(s) 867, 1389
HgaI GACGC 2 cut(s) 98, 384
HhaI GCGC 1 cut(s) 878
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 7 cut(s) 122, 334, 405, 421, 1018, 1145, 1400
Hin6I GCGC 1 cut(s) 876
HinP1I GCGC 1 cut(s) 876
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 2 cut(s) 230, 835
HpaII CCGG 2 cut(s) 867, 1389
HphI GGTGA 2 cut(s) 755, 1139
Hpy166II GTNNAC 3 cut(s) 53, 76, 399
Hpy188I TCNGA 4 cut(s) 88, 375, 925, 946
Hpy188III TCNNGA 7 cut(s) 158, 527, 575, 1191, 1266, 1301, 1343
Hpy8I GTNNAC 3 cut(s) 53, 76, 399
HpyAV CCTTC 3 cut(s) 377, 524, 1376
HpyCH4III ACNGT 3 cut(s) 201, 502, 1057
HpyCH4IV ACGT 2 cut(s) 259, 1119
HpyCH4V TGCA 7 cut(s) 82, 554, 736, 745, 1145, 1169, 1319
HpyF10VI GCNNNNNNNGC 6 cut(s) 109, 277, 367, 742, 956, 1460
HpyF3I CTNAG 6 cut(s) 223, 344, 543, 791, 951, 1259
HpySE526I ACGT 2 cut(s) 259, 1119
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 7 cut(s) 122, 334, 405, 421, 1018, 1145, 1400
HspAI GCGC 1 cut(s) 876
KpnI GGTACC 1 cut(s) 69
Kzo9I GATC 6 cut(s) 607, 692, 814, 853, 1090, 1297
LguI GCTCTTC 1 cut(s) 966
LmnI GCTCC 2 cut(s) 149, 1201
Lsp1109I GCAGC 3 cut(s) 399, 860, 1331
LweI GCATC 3 cut(s) 241, 245, 994
MaeI CTAG 4 cut(s) 272, 617, 767, 1314
MaeII ACGT 2 cut(s) 259, 1119
MaeIII GTNAC 2 cut(s) 496, 1309
MalI GATC 6 cut(s) 609, 694, 816, 855, 1092, 1299
MboI GATC 6 cut(s) 607, 692, 814, 853, 1090, 1297
MboII GAAGA 8 cut(s) 696, 953, 1049, 1055, 1192, 1348, 1495, 1515
MflI RGATCY 2 cut(s) 692, 853
MhlI GDGCHC 2 cut(s) 1157, 1206
MmeI TCCRAC 2 cut(s) 339, 828
MseI TTAA 8 cut(s) 174, 681, 900, 915, 1149, 1229, 1479, 1496
MspCI CTTAAG 1 cut(s) 899
MspI CCGG 2 cut(s) 867, 1389
Mva1269I GAATGC 2 cut(s) 554, 738
MvnI CGCG 2 cut(s) 395, 441
MwoI GCNNNNNNNGC 6 cut(s) 109, 277, 367, 742, 956, 1460
NdeII GATC 6 cut(s) 607, 692, 814, 853, 1090, 1297
NheI GCTAGC 1 cut(s) 271
NlaIII CATG 7 cut(s) 122, 334, 405, 421, 1018, 1145, 1400
NlaIV GGNNCC 4 cut(s) 67, 888, 1256, 1387
NmuCI GTSAC 1 cut(s) 496
NsbI TGCGCA 1 cut(s) 877
NspI RCATGY 2 cut(s) 334, 405
PciI ACATGT 2 cut(s) 330, 401
PciSI GCTCTTC 1 cut(s) 966
PctI GAATGC 2 cut(s) 554, 738
PfeI GAWTC 2 cut(s) 230, 835
PflFI GACNNNGTC 1 cut(s) 1397
PkrI GCNGC 4 cut(s) 196, 389, 875, 1321
Ppu21I YACGTR 2 cut(s) 260, 1120
PscI ACATGT 2 cut(s) 330, 401
PshBI ATTAAT 1 cut(s) 681
Psp124BI GAGCTC 2 cut(s) 1157, 1206
PspN4I GGNNCC 4 cut(s) 67, 888, 1256, 1387
PspPI GGNCC 1 cut(s) 887
PsuI RGATCY 2 cut(s) 692, 853
PsyI GACNNNGTC 1 cut(s) 1397
RsaI GTAC 2 cut(s) 54, 67
RsaNI GTAC 2 cut(s) 53, 66
SacI GAGCTC 2 cut(s) 1157, 1206
SapI GCTCTTC 1 cut(s) 966
SaqAI TTAA 8 cut(s) 174, 681, 900, 915, 1149, 1229, 1479, 1496
SatI GCNGC 4 cut(s) 195, 388, 874, 1320
Sau3AI GATC 6 cut(s) 607, 692, 814, 853, 1090, 1297
Sau96I GGNCC 1 cut(s) 887
SduI GDGCHC 2 cut(s) 1157, 1206
SfaNI GCATC 3 cut(s) 241, 245, 994
SfcI CTRYAG 2 cut(s) 243, 807
SgrAI CRCCGGYG 1 cut(s) 1388
SmlI CTYRAG 5 cut(s) 573, 899, 981, 1301, 1343
SmoI CTYRAG 5 cut(s) 573, 899, 981, 1301, 1343
SpeI ACTAGT 1 cut(s) 1313
SsiI CCGC 7 cut(s) 15, 21, 194, 234, 354, 393, 864
SspI AATATT 1 cut(s) 178
SspMI CTAG 4 cut(s) 272, 617, 767, 1314
SstI GAGCTC 2 cut(s) 1157, 1206
TaaI ACNGT 3 cut(s) 201, 502, 1057
TaiI ACGT 2 cut(s) 262, 1122
TaqI TCGA 2 cut(s) 817, 1407
TatI WGTACW 1 cut(s) 52
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 2 cut(s) 230, 835
Tru1I TTAA 8 cut(s) 174, 681, 900, 915, 1149, 1229, 1479, 1496
Tru9I TTAA 8 cut(s) 174, 681, 900, 915, 1149, 1229, 1479, 1496
TscAI CASTG 1 cut(s) 1249
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 3 cut(s) 387, 873, 1319
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 5 cut(s) 95, 243, 530, 681, 1086
TspRI CASTG 1 cut(s) 1249
Tth111I GACNNNGTC 1 cut(s) 1397
Vha464I CTTAAG 1 cut(s) 899
VspI ATTAAT 1 cut(s) 681
XapI RAATTY 3 cut(s) 138, 825, 1112
XceI RCATGY 2 cut(s) 334, 405
XspI CTAG 4 cut(s) 272, 617, 767, 1314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.