pycom15g13870

CCR4-associated factor 1 homolog 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016481
Physical Location & Seq
Forward (+)
9466563 .. 9467141
579 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
ATGTCTACTTTTCCAAAAAGCGATTCGATTCACATTAGGGAAGTTTGGAGCGATAATCTTGAGGAGGAGTTTAAATTGATTCGCAATATTGTAGAGGAATACCGATATGTAGCGATGGACACAGAGTTTCCCGGTATCGTTTTGAGGCCTGTAGGGACTTTCAAGGACAGTTATGATTATCACTATCAAACCCTTAAGGCCAATGTGGACCTCTTGAAGTTGATACAGTTGGGTCTCACATTTTCTGATGAGAAAGGGAACCTTCCGACCTGTGGAACCGACAAGCACTGTGTGTGGCAATTCAATTTCCGTGACTTCAACCCCAATGAGGATGTGTATGCCACTGACTCAATAGAATTACTGTCCCAGAGCGGTATGGATTTCGTGAAGAACAATGAGAAGGGTGTTGATGCTAGTAAGTTCACCGAGCTACTAATGGCATCCGGAGTTGTGCTTTCTGATAATGTGGTTTGGGTGACGTTCCACAGTGGGTATGATTTTGGGTACTTGCTCAAGCTGCTTAAAGGCCAAAGCCTCCCGAACACACAGGTGGGGTTCTTTAAAATGATCCAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

193

Amino Acids

22.04

Weight (kDa)

4.87

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 15 - 136 8e-15 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01160 FvH4_1g01160
malus_domestica MD02G1009600.v1.1
prunus_persica Prupe.7G260400_v2.0.a1
pyrus_communis pycom15g13870
rosa_chinensis RchiOBHm_Chr2g0085951
rosa_laevigata RLG00000015729
rosa_roxburghii Rroxscaffold_2G00154900
rosa_rugosa Rorug01G0464600
rosa_samantha Rh2AG013800 Rh2BG014300 Rh2CG014800 Rh2DG016100
rosa_wichuraiana Rw2G001220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 372
AccI GTMKAC 1 cut(s) 5
AccIII TCCGGA 1 cut(s) 443
AciI CCGC 1 cut(s) 372
AclWI GGATC 1 cut(s) 562
AdeI CACNNNGTG 1 cut(s) 292
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 2 cut(s) 272, 328
AflII CTTAAG 1 cut(s) 194
AgsI TTSAA 4 cut(s) 163, 217, 304, 319
AjnI CCWGG 1 cut(s) 570
AjuI GAANNNNNNNTTGG 2 cut(s) 7, 39
AleI CACNNNNGTG 1 cut(s) 548
AluBI AGCT 2 cut(s) 430, 517
AluI AGCT 2 cut(s) 430, 517
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 1 cut(s) 562
Aor13HI TCCGGA 1 cut(s) 443
AoxI GGCC 3 cut(s) 146, 198, 526
ApeKI GCWGC 1 cut(s) 517
AspS9I GGNCC 1 cut(s) 208
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 2 cut(s) 415, 487
AvaII GGWCC 1 cut(s) 208
BbvI GCAGC 1 cut(s) 504
BccI CCATC 1 cut(s) 109
BciT130I CCWGG 1 cut(s) 572
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 1 cut(s) 414
BfmI CTRYAG 1 cut(s) 150
BfrI CTTAAG 1 cut(s) 194
BisI GCNGC 1 cut(s) 518
BlsI GCNGC 1 cut(s) 519
Bme1390I CCNGG 2 cut(s) 132, 572
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 2 cut(s) 260, 277
BmrFI CCNGG 2 cut(s) 132, 572
BmsI GCATC 2 cut(s) 400, 449
BpuEI CTTGAG 2 cut(s) 80, 497
BpuMI CCSGG 1 cut(s) 132
BsaI GGTCTC 1 cut(s) 239
BsaWI WCCGGW 1 cut(s) 443
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 328
BseAI TCCGGA 1 cut(s) 443
BseBI CCWGG 1 cut(s) 572
BseGI GGATG 2 cut(s) 337, 440
BseLI CCNNNNNNNGG 2 cut(s) 272, 328
BseRI GAGGAG 2 cut(s) 77, 80
BseXI GCAGC 1 cut(s) 504
BshFI GGCC 3 cut(s) 148, 200, 528
BsiSI CCGG 2 cut(s) 132, 444
BslFI GGGAC 2 cut(s) 169, 349
BslI CCNNNNNNNGG 2 cut(s) 272, 328
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 2 cut(s) 169, 349
BsnI GGCC 3 cut(s) 148, 200, 528
Bso31I GGTCTC 1 cut(s) 239
Bsp13I TCCGGA 1 cut(s) 443
Bsp143I GATC 1 cut(s) 567
BspACI CCGC 1 cut(s) 372
BspANI GGCC 3 cut(s) 148, 200, 528
BspEI TCCGGA 1 cut(s) 443
BspLI GGNNCC 2 cut(s) 260, 277
BspPI GGATC 1 cut(s) 562
BspTI CTTAAG 1 cut(s) 194
BspTNI GGTCTC 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 372
BssMI GATC 1 cut(s) 567
Bst2UI CCWGG 1 cut(s) 572
Bst4CI ACNGT 5 cut(s) 170, 228, 290, 363, 488
BstAFI CTTAAG 1 cut(s) 194
BstF5I GGATG 2 cut(s) 337, 440
BstKTI GATC 1 cut(s) 570
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 1 cut(s) 567
BstMWI GCNNNNNNNGC 1 cut(s) 517
BstNI CCWGG 1 cut(s) 572
BstSCI CCNGG 2 cut(s) 130, 570
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 1 cut(s) 504
BsuRI GGCC 3 cut(s) 148, 200, 528
BtgZI GCGATG 1 cut(s) 128
BtsCI GGATG 2 cut(s) 337, 440
BtsIMutI CAGTG 3 cut(s) 286, 342, 493
Cfr13I GGNCC 1 cut(s) 208
Csp6I GTAC 1 cut(s) 505
CviJI RGCY 6 cut(s) 148, 200, 430, 517, 528, 534
CviKI_1 RGCY 6 cut(s) 148, 200, 430, 517, 528, 534
CviQI GTAC 1 cut(s) 505
DpnI GATC 1 cut(s) 569
DpnII GATC 1 cut(s) 567
DraI TTTAAA 2 cut(s) 73, 562
DraIII CACNNNGTG 1 cut(s) 292
Eco147I AGGCCT 1 cut(s) 148
Eco31I GGTCTC 1 cut(s) 239
Eco47I GGWCC 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 570
FaiI YATR 5 cut(s) 108, 174, 339, 377, 495
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FaqI GGGAC 2 cut(s) 169, 349
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 518
FokI GGATG 2 cut(s) 344, 427
Fsp4HI GCNGC 1 cut(s) 518
FspBI CTAG 1 cut(s) 414
GluI GCNGC 1 cut(s) 518
HaeIII GGCC 3 cut(s) 148, 200, 528
HapII CCGG 2 cut(s) 132, 444
HinfI GANTC 4 cut(s) 23, 28, 79, 347
HpaII CCGG 2 cut(s) 132, 444
HphI GGTGA 2 cut(s) 415, 487
Hpy166II GTNNAC 3 cut(s) 6, 208, 423
Hpy188I TCNGA 3 cut(s) 247, 267, 460
Hpy188III TCNNGA 5 cut(s) 59, 214, 385, 444, 538
Hpy8I GTNNAC 3 cut(s) 6, 208, 423
HpyAV CCTTC 2 cut(s) 272, 394
HpyCH4III ACNGT 5 cut(s) 170, 228, 290, 363, 488
HpyCH4IV ACGT 1 cut(s) 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 517
HpySE526I ACGT 1 cut(s) 479
Kpn2I TCCGGA 1 cut(s) 443
Kzo9I GATC 1 cut(s) 567
LmnI GCTCC 1 cut(s) 48
LpnPI CCDG 7 cut(s) 145, 162, 283, 380, 457, 533, 557
Lsp1109I GCAGC 1 cut(s) 504
LweI GCATC 2 cut(s) 400, 449
MaeI CTAG 1 cut(s) 414
MaeII ACGT 1 cut(s) 479
MaeIII GTNAC 2 cut(s) 311, 475
MalI GATC 1 cut(s) 569
MbiI CCGCTC 1 cut(s) 372
MboI GATC 1 cut(s) 567
MboII GAAGA 1 cut(s) 400
MluCI AATT 4 cut(s) 74, 299, 304, 356
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 290
MnlI CCTC 7 cut(s) 55, 58, 88, 138, 221, 322, 545
MroI TCCGGA 1 cut(s) 443
MseI TTAA 4 cut(s) 72, 195, 522, 561
MslI CAYNNNNRTG 1 cut(s) 548
MspCI CTTAAG 1 cut(s) 194
MspI CCGG 2 cut(s) 132, 444
MspR9I CCNGG 2 cut(s) 132, 572
MvaI CCWGG 1 cut(s) 572
MwoI GCNNNNNNNGC 1 cut(s) 517
NciI CCSGG 1 cut(s) 132
NdeII GATC 1 cut(s) 567
NlaIV GGNNCC 2 cut(s) 260, 277
NmuCI GTSAC 2 cut(s) 311, 475
OliI CACNNNNGTG 1 cut(s) 548
PceI AGGCCT 1 cut(s) 148
PfeI GAWTC 3 cut(s) 23, 28, 79
PkrI GCNGC 1 cut(s) 519
PleI GAGTC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 570
PspGI CCWGG 1 cut(s) 570
PspN4I GGNNCC 2 cut(s) 260, 277
PspPI GGNCC 1 cut(s) 208
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
RseI CAYNNNNRTG 1 cut(s) 548
SaqAI TTAA 4 cut(s) 72, 195, 522, 561
SatI GCNGC 1 cut(s) 518
Sau3AI GATC 1 cut(s) 567
Sau96I GGNCC 1 cut(s) 208
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 2 cut(s) 132, 572
SetI ASST 8 cut(s) 213, 264, 272, 432, 482, 519, 552, 576
SfaNI GCATC 2 cut(s) 400, 449
SfcI CTRYAG 1 cut(s) 150
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 3 cut(s) 59, 194, 512
SmoI CTYRAG 3 cut(s) 59, 194, 512
Sse9I AATT 4 cut(s) 74, 299, 304, 356
SseBI AGGCCT 1 cut(s) 148
SsiI CCGC 1 cut(s) 372
SspI AATATT 1 cut(s) 88
SspMI CTAG 1 cut(s) 414
StuI AGGCCT 1 cut(s) 148
StyD4I CCNGG 2 cut(s) 130, 570
TaaI ACNGT 5 cut(s) 170, 228, 290, 363, 488
TaiI ACGT 1 cut(s) 482
TaqI TCGA 1 cut(s) 26
TasI AATT 4 cut(s) 74, 299, 304, 356
TfiI GAWTC 3 cut(s) 23, 28, 79
Tru1I TTAA 4 cut(s) 72, 195, 522, 561
Tru9I TTAA 4 cut(s) 72, 195, 522, 561
TscAI CASTG 3 cut(s) 293, 349, 493
TseFI GTSAC 2 cut(s) 311, 475
TseI GCWGC 1 cut(s) 517
Tsp45I GTSAC 2 cut(s) 311, 475
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 3 cut(s) 293, 349, 493
Vha464I CTTAAG 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 208
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.