pycom15g14100

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016719
Physical Location & Seq
Reverse (-)
9606890 .. 9609809
2920 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 606 bp
ATGCCTCGCGCTTCTAAGAGAAAAGCACCTCCACCAACTTCGCCTTCCCTCGCGTCCTGCGACGTCGTTTCGAAGCGCGCCGCAACAGGAATCGAGGCTCTTTGTTCGGACTTGAAAGTGGACCATACCGATGTTAGAATACTGATGCTTGCTTGGAAAATGAGAGCTGCAAAGCAGGGCTACTTTTCAAAGGATGAGTGGCAAAGAGGCTTGAAAGATCTACATGCCGACACTATCCCTAAATTAAAGAAAGCACTACCGGGATTGGAGAAAGAGTTGACAACAGCATCCAAATTTGAAGATTTCTATGCCTTCGCATTTCAATACTGCTTGACAGAGGAAAGGCAAAAGAGTGTGGACATTGAGACTGTGTGTGAATTACTGAATCTCGTACTGGGTCCTCGATACCAGCTCCAGGTTGATTTATTGATCAAGTATTTGAAGGTCCAAACTGAGTACAGGGCACTAAATATGGATCAGTGGATGCATTTTTACCGTTTTTGCAATGAGATAAGTTTTCCAGACCTGAAAAATTATGATTCTGATCAAGCATGGCCGGTGATTTTTGATAACTTTGTTGAATGGGTGAGAGACAAGCAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

202

Amino Acids

23.4

Weight (kDa)

7.59

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 81 - 195 4.1e-29 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016719)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01430 FvH4_1g01430
malus_domestica MD15G1157200.v1.1
prunus_persica Prupe.7G258300_v2.0.a1 Prupe.7G258300_v2.0.a1
pyrus_communis pycom15g14100
rosa_chinensis RchiOBHm_Chr2g0086341
rosa_laevigata RLG00000015751
rosa_multiflora Rmu_sc0003572.1_g000003 Rmu_sc0004510.1_g000020
rosa_roxburghii Rroxscaffold_2G00154680
rosa_rugosa Rorug01G0466700
rosa_samantha Rh2AG016900
rosa_wichuraiana Rw2G001430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 66
AccII CGCG 3 cut(s) 9, 53, 78
AciI CCGC 1 cut(s) 81
AclWI GGATC 1 cut(s) 483
AcoI YGGCCR 1 cut(s) 554
AcsI RAATTY 1 cut(s) 293
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 2 cut(s) 393, 458
AfiI CCNNNNNNNGG 1 cut(s) 415
AgsI TTSAA 7 cut(s) 115, 189, 214, 299, 323, 442, 581
AjnI CCWGG 1 cut(s) 414
AjuI GAANNNNNNNTTGG 2 cut(s) 28, 60
AluBI AGCT 3 cut(s) 167, 412, 603
AluI AGCT 3 cut(s) 167, 412, 603
Alw26I GTCTC 2 cut(s) 359, 585
AlwI GGATC 1 cut(s) 483
AoxI GGCC 1 cut(s) 554
ApeKI GCWGC 1 cut(s) 167
ApoI RAATTY 1 cut(s) 293
AspLEI GCGC 3 cut(s) 11, 78, 80
AspS9I GGNCC 3 cut(s) 121, 398, 445
AsuC2I CCSGG 1 cut(s) 261
AsuHPI GGTGA 2 cut(s) 571, 598
AsuII TTCGAA 1 cut(s) 71
AvaII GGWCC 3 cut(s) 121, 398, 445
BaeGI GKGCMC 1 cut(s) 466
BbvI GCAGC 1 cut(s) 154
BciT130I CCWGG 1 cut(s) 416
BclI TGATCA 2 cut(s) 429, 544
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 2 cut(s) 359, 585
BglII AGATCT 1 cut(s) 217
BisI GCNGC 2 cut(s) 81, 168
BlsI GCNGC 2 cut(s) 82, 169
Bme1390I CCNGG 2 cut(s) 261, 416
Bme18I GGWCC 3 cut(s) 121, 398, 445
BmgT120I GGNCC 3 cut(s) 121, 398, 445
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 2 cut(s) 261, 416
BmrI ACTGGG 1 cut(s) 404
BmsI GCATC 3 cut(s) 135, 296, 474
BmuI ACTGGG 1 cut(s) 404
BpmI CTGGAG 1 cut(s) 398
Bpu14I TTCGAA 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 261
BsaBI GATNNNNATC 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse118I RCCGGY 1 cut(s) 556
Bse1I ACTGG 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 511
Bse8I GATNNNNATC 1 cut(s) 543
BseBI CCWGG 1 cut(s) 416
BseGI GGATG 3 cut(s) 199, 287, 489
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMI GCAATG 1 cut(s) 511
BseMII CTCAG 1 cut(s) 444
BseNI ACTGG 1 cut(s) 399
BsePI GCGCGC 1 cut(s) 76
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 1 cut(s) 154
Bsh1236I CGCG 3 cut(s) 9, 53, 78
BshFI GGCC 1 cut(s) 556
BsiSI CCGG 2 cut(s) 260, 557
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 2 cut(s) 359, 585
BsnI GGCC 1 cut(s) 556
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 4 cut(s) 217, 429, 475, 544
BspACI CCGC 1 cut(s) 81
BspANI GGCC 1 cut(s) 556
BspCNI CTCAG 1 cut(s) 445
BspFNI CGCG 3 cut(s) 9, 53, 78
BspLI GGNNCC 1 cut(s) 399
BspPI GGATC 1 cut(s) 483
BspT104I TTCGAA 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 511
BsrFI RCCGGY 1 cut(s) 556
BsrI ACTGG 1 cut(s) 399
BssAI RCCGGY 1 cut(s) 556
BssHII GCGCGC 1 cut(s) 76
BssMI GATC 4 cut(s) 217, 429, 475, 544
BssNI GRCGYC 1 cut(s) 63
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 2 cut(s) 370, 497
BstACI GRCGYC 1 cut(s) 63
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 78, 150
BstDEI CTNAG 2 cut(s) 15, 453
BstF5I GGATG 3 cut(s) 199, 287, 489
BstFNI CGCG 3 cut(s) 9, 53, 78
BstHHI GCGC 3 cut(s) 11, 78, 80
BstKTI GATC 4 cut(s) 220, 432, 478, 547
BstMAI GTCTC 2 cut(s) 359, 585
BstMBI GATC 4 cut(s) 217, 429, 475, 544
BstNI CCWGG 1 cut(s) 416
BstNSI RCATGY 1 cut(s) 227
BstSCI CCNGG 2 cut(s) 259, 414
BstSLI GKGCMC 1 cut(s) 466
BstUI CGCG 3 cut(s) 9, 53, 78
BstV1I GCAGC 1 cut(s) 154
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuRI GGCC 1 cut(s) 556
BtsCI GGATG 3 cut(s) 199, 287, 489
BtsIMutI CAGTG 1 cut(s) 485
Cac8I GCNNGC 2 cut(s) 78, 150
CfoI GCGC 3 cut(s) 11, 78, 80
Cfr10I RCCGGY 1 cut(s) 556
Cfr13I GGNCC 3 cut(s) 121, 398, 445
CseI GACGC 1 cut(s) 42
Csp6I GTAC 2 cut(s) 392, 457
CviAII CATG 2 cut(s) 224, 552
CviJI RGCY 7 cut(s) 98, 167, 180, 210, 412, 556, 603
CviKI_1 RGCY 7 cut(s) 98, 167, 180, 210, 412, 556, 603
CviQI GTAC 2 cut(s) 392, 457
DdeI CTNAG 2 cut(s) 15, 453
DpnI GATC 4 cut(s) 219, 431, 477, 546
DpnII GATC 4 cut(s) 217, 429, 475, 544
EaeI YGGCCR 1 cut(s) 554
Eco47I GGWCC 3 cut(s) 121, 398, 445
EcoO109I RGGNCCY 1 cut(s) 398
EcoRII CCWGG 1 cut(s) 414
EcoT22I ATGCAT 1 cut(s) 489
FaeI CATG 2 cut(s) 227, 555
FaiI YATR 6 cut(s) 126, 225, 309, 473, 537, 553
FatI CATG 2 cut(s) 223, 551
FbaI TGATCA 2 cut(s) 429, 544
Fnu4HI GCNGC 2 cut(s) 81, 168
FokI GGATG 3 cut(s) 206, 274, 496
Fsp4HI GCNGC 2 cut(s) 81, 168
GlaI GCGC 3 cut(s) 10, 77, 79
GluI GCNGC 2 cut(s) 81, 168
GsuI CTGGAG 1 cut(s) 398
HaeIII GGCC 1 cut(s) 556
HapII CCGG 2 cut(s) 260, 557
HgaI GACGC 1 cut(s) 42
HhaI GCGC 3 cut(s) 11, 78, 80
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 2 cut(s) 227, 555
Hin6I GCGC 3 cut(s) 9, 76, 78
HinP1I GCGC 3 cut(s) 9, 76, 78
HincII GTYRAC 1 cut(s) 279
HindII GTYRAC 1 cut(s) 279
HinfI GANTC 3 cut(s) 90, 385, 539
HpaII CCGG 2 cut(s) 260, 557
HphI GGTGA 2 cut(s) 571, 598
Hpy166II GTNNAC 3 cut(s) 121, 279, 358
Hpy188I TCNGA 2 cut(s) 109, 544
Hpy188III TCNNGA 1 cut(s) 521
Hpy8I GTNNAC 3 cut(s) 121, 279, 358
Hpy99I CGWCG 2 cut(s) 65, 68
HpyAV CCTTC 3 cut(s) 54, 322, 436
HpyCH4III ACNGT 2 cut(s) 370, 497
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 3 cut(s) 170, 487, 504
HpyF3I CTNAG 2 cut(s) 15, 453
HpySE526I ACGT 1 cut(s) 63
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 2 cut(s) 227, 555
HspAI GCGC 3 cut(s) 9, 76, 78
Ksp22I TGATCA 2 cut(s) 429, 544
Kzo9I GATC 4 cut(s) 217, 429, 475, 544
LmnI GCTCC 1 cut(s) 417
Lsp1109I GCAGC 1 cut(s) 154
LweI GCATC 3 cut(s) 135, 296, 474
MaeII ACGT 1 cut(s) 63
MalI GATC 4 cut(s) 219, 431, 477, 546
MboI GATC 4 cut(s) 217, 429, 475, 544
MboII GAAGA 1 cut(s) 311
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 4 cut(s) 242, 293, 377, 532
MnlI CCTC 7 cut(s) 15, 39, 59, 88, 200, 331, 411
Mph1103I ATGCAT 1 cut(s) 489
MseI TTAA 1 cut(s) 245
MslI CAYNNNNRTG 1 cut(s) 129
MspI CCGG 2 cut(s) 260, 557
MspR9I CCNGG 2 cut(s) 261, 416
MvaI CCWGG 1 cut(s) 416
MvnI CGCG 3 cut(s) 9, 53, 78
NciI CCSGG 1 cut(s) 261
NdeII GATC 4 cut(s) 217, 429, 475, 544
NlaIII CATG 2 cut(s) 227, 555
NlaIV GGNNCC 1 cut(s) 399
NsiI ATGCAT 1 cut(s) 489
NspI RCATGY 1 cut(s) 227
NspV TTCGAA 1 cut(s) 71
PauI GCGCGC 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PfeI GAWTC 3 cut(s) 90, 385, 539
PkrI GCNGC 2 cut(s) 82, 169
PpuMI RGGWCCY 1 cut(s) 398
Psp5II RGGWCCY 1 cut(s) 398
Psp6I CCWGG 1 cut(s) 414
PspGI CCWGG 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 3 cut(s) 121, 398, 445
PspPPI RGGWCCY 1 cut(s) 398
PsuI RGATCY 1 cut(s) 217
PteI GCGCGC 1 cut(s) 76
RsaI GTAC 2 cut(s) 393, 458
RsaNI GTAC 2 cut(s) 392, 457
RseI CAYNNNNRTG 1 cut(s) 129
SaqAI TTAA 1 cut(s) 245
SatI GCNGC 2 cut(s) 81, 168
Sau3AI GATC 4 cut(s) 217, 429, 475, 544
Sau96I GGNCC 3 cut(s) 121, 398, 445
ScrFI CCNGG 2 cut(s) 261, 416
SduI GDGCHC 1 cut(s) 466
SetI ASST 8 cut(s) 31, 66, 169, 414, 420, 447, 528, 605
SfaNI GCATC 3 cut(s) 135, 296, 474
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 3 cut(s) 121, 398, 445
SmiMI CAYNNNNRTG 1 cut(s) 129
Sse9I AATT 4 cut(s) 242, 293, 377, 532
SsiI CCGC 1 cut(s) 81
StyD4I CCNGG 2 cut(s) 259, 414
TaaI ACNGT 2 cut(s) 370, 497
TaiI ACGT 1 cut(s) 66
TaqI TCGA 3 cut(s) 71, 93, 403
TasI AATT 4 cut(s) 242, 293, 377, 532
TatI WGTACW 1 cut(s) 456
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 3 cut(s) 90, 385, 539
Tru1I TTAA 1 cut(s) 245
Tru9I TTAA 1 cut(s) 245
TscAI CASTG 1 cut(s) 485
TseI GCWGC 1 cut(s) 167
TspRI CASTG 1 cut(s) 485
VpaK11BI GGWCC 3 cut(s) 121, 398, 445
XapI RAATTY 1 cut(s) 293
XceI RCATGY 1 cut(s) 227
ZraI GACGTC 1 cut(s) 64
Zsp2I ATGCAT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.