Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0015953

Physical Location & Seq

Genomic Coordinates Reverse (-)

9619472 .. 9624519

Gene Structure 5048 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2256 bp

ATGTGGTCGAATATTTTAATTGATAGGAGAGAGCATAGAGCAGAGAGCAGAGTCATGTCAGGGTGGGACGAAGGAGCGATCTATTACAGCGACCAAGCGCAGTCGCTCGGCGGCGACGGCGGAGACCCAGGAAGCAACGCCGCAGCTGCTACCCGCCACTCGGTCCTCCAGAAATTCAAGGAATTCATCCGAAGCTTTGAGACCCAGAAAAATGTGTTTTCCTACAGGGAGAGCCTCCTCCACAACCCTAAGTACCTTCTCGTCGACATTGAAGACCTCGATTCTTTCGACGCCGACCTCGTCGCAAGGCTCCGCTCTGCCCCCTCCGATTACTTGCCTCTGTTTGAGAACGCCGCCGGTCAGGTTTTGGCGAATTTGAAGACGAAAGTTCCGGGAAACGAAGGGAAATTGGAGGAACGGGTGCCGGAGGATGTTCAGATCTTGCTGACCTCCAAAGAGGACCCGGCGTCCATGCGCCAGCTCGGGGCACAATGTATATCAAAACTCATCAAGGTTTCCGGGATCACTATAGCTGCTTCGAGGGTCAAGGCGAAGGCGACTTATGTGATTGTCATGTGTAAGAACTGTAAAAATGTGCAGAGGGTACCTTGCCGGCCAGGTCTTGGTGGGGCAATCGTCCCTCGCTCTTGCAACCATATTCCTCAGCCTGGAGAAGAACCTTGTCCGCTTGATCCGTGGGTTGTGGTTCCTGATAAGAGCAAGTATGTTGATCAGCAAACCTTGAAATTGCAAGAGAATCCAGAGGATGTACCTACCGGAGAGCTTCCAAGAAATATGCTTCTATCTGTGGACCGTCATCTTGTTCAGACAATTGTGCCTGGCACAAGATTAACCATCATGGGCATTTATAGCATCTATCAAGCTTCCAATTCTGCCACATCTCACAAAGGAGCAGTTGCTGTTAGGCAGCCTTACATCAGAGTGGTAGGAATAGAAGAAGCAACTGATGCCAACTCTCGCGGTCCCGCTGCTTTTACACAAGACGAGATAGAAGAATTCAAGAAGTTTGCTTCAGAAGATGCTTATAAAAATATATGCTCGAAAATTGCTCCCTCTATATTCGGTCATGAAGATGTAAAGAAGGCTGTGGCGTGTCTTCTGTTCGGGGGATCTAGAAAGCATTTGCCTGATGGTGTGAAGCTAAGAGGTGATATTAATGTCTTACTTTTGGGGGATCCATCTACCGCTAAATCACAGTTTCTTAAGTTCGTCGAGAAGACAGCTCCGGTGGCCGTATATACTTCAGGAAAAGGGTCATCAGCTGCTGGTCTTACAGCTTCTGTGATCAGGGATAGCAGTTCTCGTGAGTTTTATCTTGAAGGAGGAGCCATGGTTTTAGCGGATGGAGGTGTTGTCTGTATTGACGAGTTTGACAAAATGAGATCAGAGGATAGGGTTGCTATTCATGAAGCCATGGAGCAGCAAACCATATCCATTGCCAAAGCAGGAATAACAACCGTTCTTAATTCTAGAACCTCTGTGCTTGCAGCTGCTAACCCTCCATCTGGACGTTATGACGATCTTAAGACTGCTCAGGATAATATCGATCTGCAGACGACAATTCTTTCGAGATTTGACTTGATATTCATTGTTAAAGATATCAGGATGTACAGTCAGGATAAGATTATAGCAAGTCATATCATAAAGGTTCACGCGTCTGCTGGTGCAGCACTTGGTGATGGTAGAGTAGTTTCTAAAGAAGAGAATTGGCTGAAGAGGTACATACAATATTGTCGAACTGAATGCCACCCCCGTTTATCAGAAACTGCGTCGAGAGAGCTGCAGAGTAATTATGTGAAAATCAGACAGGACATGAGACAGCTAGCAAATGAAACCGGAGAGGCTGCTGCAGTCCCAATCACCGTAAGGCAGCTTGAAGCTATAGTGAGGTTGAGTGAGGCCCTTGCGAAAATGCAACTGCGCCACGAAGCTACTGAGGAGCATGTTCGTGAAGCAATAAGACTATTCAATGCAGCCACAATGGACGCGGCAAAGTCTGGAATACAGCAACAAGTCAATCTCACTGGCGAGATGGCGAATGAAGTTAAGCAAGCTGAAACTCAGATAAGGAGAAGAGTAGGCATTGGGAACCACATATCAGAGAGAAAGCTGATCGATGAGCTCACAAGAATGGGGATGAATGAATCCATTGTGAGAAGAGCTCTTATTATTATGCACCAGAGGGATGAAGTCGAATACAAGCGAGAAAGACGCGTTATTTTACGCAAAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 ko05206 map03030 map04110 map04111 map04113 map05206

Pfam Domains

Protein Families

MCM MCM_N MCM_OB Mg_chelatase

Protein Analysis

752

Amino Acids

83.21

Weight (kDa)

8.31

Isoelectric Point (pI)

48.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	57 - 143	1e-12	MCM N-terminal domain
MCM_OB	PF17207	158 - 293	2.8e-34	MCM OB domain
MCM	PF00493	337 - 558	8.7e-101	MCM P-loop domain
MCM_lid	PF17855	577 - 666	1.1e-28	MCM AAA-lid domain
MCM5_C	PF21933	691 - 749	8.3e-18	MCM5, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0015953)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G07690 AT2G07690
fragaria_vesca	FvH4_1g01490
malus_domestica	MD02G1011900.v1.1 MD15G1157500.v1.1
prunus_persica	Prupe.7G252700_v2.0.a1
pyrus_communis	pycom15g14130
rosa_chinensis	RchiOBHm_Chr2g0086411
rosa_laevigata	RLG00000015756
rosa_multiflora	Rmu_sc0005127.1_g000006
rosa_roxburghii	Rroxscaffold_2G00154600
rosa_rugosa	Rorug01G0467200
rosa_samantha	Rh2AG017800 Rh2DG019100
rosa_wichuraiana	Rw2G001480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1047
Acc65I	GGTACC	1 cut(s)	604
AccB1I	GGYRCC	2 cut(s)	421, 604
AccB7I	CCANNNNNTGG	1 cut(s)	623
AccBSI	CCGCTC	1 cut(s)	315
AccI	GTMKAC	1 cut(s)	264
AccII	CGCG	4 cut(s)	981, 1676, 2009, 2235
AclWI	GGATC	5 cut(s)	530, 686, 1138, 1190, 1203
AcoI	YGGCCR	2 cut(s)	614, 1251
AcsI	RAATTY	4 cut(s)	173, 182, 373, 1016
AcuI	CTGAAG	3 cut(s)	1017, 1248, 1754
AcyI	GRCGYC	2 cut(s)	291, 467
AdeI	CACNNNGTG	1 cut(s)	1697
AfaI	GTAC	5 cut(s)	254, 606, 771, 1631, 1742
AfiI	CCNNNNNNNGG	5 cut(s)	160, 484, 623, 668, 1526
AflII	CTTAAG	2 cut(s)	1223, 1544
AflIII	ACRYGT	2 cut(s)	1674, 2233
AgsI	TTSAA	8 cut(s)	178, 272, 379, 745, 1021, 1340, 1898, 1990
AhdI	GACNNNNNGTC	1 cut(s)	466
AjnI	CCWGG	4 cut(s)	127, 616, 667, 838
Alw21I	GWGCWC	2 cut(s)	2145, 2185
Alw26I	GTCTC	3 cut(s)	117, 194, 1831
AlwI	GGATC	5 cut(s)	530, 686, 1138, 1190, 1203
AlwNI	CAGNNNCTG	4 cut(s)	920, 1286, 1301, 1787
Ama87I	CYCGRG	1 cut(s)	482
AoxI	GGCC	3 cut(s)	614, 1251, 1920
ApoI	RAATTY	4 cut(s)	173, 182, 373, 1016
ArsI	GACNNNNNNTTYG	2 cut(s)	1570, 1602
AseI	ATTAAT	1 cut(s)	1176
Asp718I	GGTACC	1 cut(s)	604
AspLEI	GCGC	3 cut(s)	100, 477, 1944
AspS9I	GGNCC	5 cut(s)	163, 460, 811, 983, 1921
AsuC2I	CCSGG	3 cut(s)	393, 464, 520
AsuHPI	GGTGA	3 cut(s)	1181, 1709, 1873
AsuNHI	GCTAGC	1 cut(s)	1843
AvaI	CYCGRG	1 cut(s)	482
AvaII	GGWCC	4 cut(s)	163, 460, 811, 983
BaeGI	GKGCMC	1 cut(s)	490
BamHI	GGATCC	1 cut(s)	1195
BanI	GGYRCC	2 cut(s)	421, 604
BanII	GRGCYC	2 cut(s)	2145, 2185
BauI	CACGAG	1 cut(s)	1323
BbsI	GAAGAC	4 cut(s)	279, 386, 1109, 1244
Bbv12I	GWGCWC	2 cut(s)	2145, 2185
BbvCI	CCTCAGC	1 cut(s)	663
BccI	CCATC	7 cut(s)	863, 1145, 1207, 1358, 1531, 1694, 2047
BceAI	ACGGC	2 cut(s)	133, 1238
BcgI	CGANNNNNNTGC	4 cut(s)	1746, 1780, 1783, 1817
BciT130I	CCWGG	4 cut(s)	129, 618, 669, 840
BclI	TGATCA	2 cut(s)	730, 1305
BcnI	CCSGG	3 cut(s)	393, 464, 520
BcoDI	GTCTC	3 cut(s)	117, 194, 1831
BfaI	CTAG	3 cut(s)	1134, 1491, 1844
BfmI	CTRYAG	6 cut(s)	223, 528, 1571, 1802, 1869, 1902
BfrI	CTTAAG	2 cut(s)	1223, 1544
BglII	AGATCT	1 cut(s)	438
Bme1390I	CCNGG	7 cut(s)	129, 393, 464, 520, 618, 669, 840
Bme18I	GGWCC	4 cut(s)	163, 460, 811, 983
BmeRI	GACNNNNNGTC	1 cut(s)	466
BmeT110I	CYCGRG	1 cut(s)	482
BmgT120I	GGNCC	5 cut(s)	163, 460, 811, 983, 1921
BmiI	GGNNCC	9 cut(s)	311, 423, 462, 606, 708, 985, 1197, 1348, 2111
BmrFI	CCNGG	7 cut(s)	129, 393, 464, 520, 618, 669, 840
BmsI	GCATC	3 cut(s)	882, 958, 1030
BmtI	GCTAGC	1 cut(s)	1847
BpiI	GAAGAC	4 cut(s)	279, 386, 1109, 1244
BplI	GAGNNNNNCTC	2 cut(s)	2167, 2199
BpmI	CTGGAG	2 cut(s)	152, 690
Bpu10I	CCTNAGC	2 cut(s)	663, 1554
BpuMI	CCSGG	3 cut(s)	393, 464, 520
Bsa29I	ATCGAT	2 cut(s)	1566, 2136
BsaHI	GRCGYC	2 cut(s)	291, 467
BsaI	GGTCTC	2 cut(s)	117, 194
BsaJI	CCNNGG	4 cut(s)	127, 695, 1350, 1434
BsaWI	WCCGGW	3 cut(s)	776, 1246, 1856
BsaXI	ACNNNNNCTCC	4 cut(s)	1338, 1359, 1368, 1389
Bsc4I	CCNNNNNNNGG	5 cut(s)	160, 484, 623, 668, 1526
Bse118I	RCCGGY	2 cut(s)	356, 612
Bse1I	ACTGG	1 cut(s)	2050
Bse3DI	GCAATG	1 cut(s)	1455
BseBI	CCWGG	4 cut(s)	129, 618, 669, 840
BseCI	ATCGAT	2 cut(s)	1566, 2136
BseDI	CCNNGG	4 cut(s)	127, 695, 1350, 1434
BseGI	GGATG	7 cut(s)	186, 436, 772, 1369, 1632, 2163, 2212
BseLI	CCNNNNNNNGG	5 cut(s)	160, 484, 623, 668, 1526
BseMI	GCAATG	1 cut(s)	1455
BseMII	CTCAG	4 cut(s)	677, 1568, 1947, 2096
BseNI	ACTGG	1 cut(s)	2050
BseRI	GAGGAG	3 cut(s)	227, 1359, 1973
BseSI	GKGCMC	1 cut(s)	490
BsgI	GTGCAG	2 cut(s)	617, 1707
Bsh1236I	CGCG	4 cut(s)	981, 1676, 2009, 2235
BshFI	GGCC	3 cut(s)	616, 1253, 1922
BshNI	GGYRCC	2 cut(s)	421, 604
BshVI	ATCGAT	2 cut(s)	1566, 2136
BsiHKAI	GWGCWC	2 cut(s)	2145, 2185
BsiHKCI	CYCGRG	1 cut(s)	482
BsiSI	CCGG	9 cut(s)	357, 392, 425, 464, 519, 613, 777, 1247, 1857
BslFI	GGGAC	4 cut(s)	80, 623, 969, 1859
BslI	CCNNNNNNNGG	5 cut(s)	160, 484, 623, 668, 1526
BsmAI	GTCTC	3 cut(s)	117, 194, 1831
BsmFI	GGGAC	4 cut(s)	80, 623, 969, 1859
BsmI	GAATGC	1 cut(s)	1769
BsnI	GGCC	3 cut(s)	616, 1253, 1922
Bso31I	GGTCTC	2 cut(s)	117, 194
BsoBI	CYCGRG	1 cut(s)	482
Bsp1286I	GDGCHC	3 cut(s)	490, 2145, 2185
Bsp1407I	TGTACA	1 cut(s)	1629
Bsp19I	CCATGG	2 cut(s)	1350, 1434
BspANI	GGCC	3 cut(s)	616, 1253, 1922
BspCNI	CTCAG	4 cut(s)	676, 1567, 1948, 2095
BspDI	ATCGAT	2 cut(s)	1566, 2136
BspFNI	CGCG	4 cut(s)	981, 1676, 2009, 2235
BspHI	TCATGA	2 cut(s)	1087, 1426
BspLI	GGNNCC	9 cut(s)	311, 423, 462, 606, 708, 985, 1197, 1348, 2111
BspMAI	CTGCAG	3 cut(s)	1575, 1806, 1873
BspOI	GCTAGC	1 cut(s)	1847
BspPI	GGATC	5 cut(s)	530, 686, 1138, 1190, 1203
BspQI	GCTCTTC	1 cut(s)	2173
BspT107I	GGYRCC	2 cut(s)	421, 604
BspTI	CTTAAG	2 cut(s)	1223, 1544
BspTNI	GGTCTC	2 cut(s)	117, 194
BsrBI	CCGCTC	1 cut(s)	315
BsrDI	GCAATG	1 cut(s)	1455
BsrFI	RCCGGY	2 cut(s)	356, 612
BsrGI	TGTACA	1 cut(s)	1629
BsrI	ACTGG	1 cut(s)	2050
BssAI	RCCGGY	2 cut(s)	356, 612
BssECI	CCNNGG	4 cut(s)	127, 695, 1350, 1434
BssNI	GRCGYC	2 cut(s)	291, 467
BssSI	CACGAG	1 cut(s)	1323
BssT1I	CCWWGG	2 cut(s)	1350, 1434
Bst2BI	CACGAG	1 cut(s)	1323
Bst2UI	CCWGG	4 cut(s)	129, 618, 669, 840
Bst4CI	ACNGT	6 cut(s)	587, 815, 1218, 1480, 1634, 1885
Bst6I	CTCTTC	4 cut(s)	1716, 1730, 2089, 2173
BstACI	GRCGYC	2 cut(s)	291, 467
BstAFI	CTTAAG	2 cut(s)	1223, 1544
BstAPI	GCANNNNNTGC	1 cut(s)	968
BstAUI	TGTACA	1 cut(s)	1629
BstC8I	GCNNGC	5 cut(s)	479, 614, 1506, 1845, 2073
BstDEI	CTNAG	6 cut(s)	249, 663, 1163, 1554, 1956, 2082
BstDSI	CCRYGG	3 cut(s)	695, 1350, 1434
BstF5I	GGATG	7 cut(s)	186, 436, 772, 1369, 1632, 2163, 2212
BstFNI	CGCG	4 cut(s)	981, 1676, 2009, 2235
BstHHI	GCGC	3 cut(s)	100, 477, 1944
BstMAI	GTCTC	3 cut(s)	117, 194, 1831
BstMWI	GCNNNNNNNGC	6 cut(s)	117, 146, 870, 968, 1250, 1688
BstNI	CCWGG	4 cut(s)	129, 618, 669, 840
BstNSI	RCATGY	1 cut(s)	1967
BstSCI	CCNGG	7 cut(s)	127, 391, 462, 518, 616, 667, 838
BstSFI	CTRYAG	6 cut(s)	223, 528, 1571, 1802, 1869, 1902
BstSLI	GKGCMC	1 cut(s)	490
BstUI	CGCG	4 cut(s)	981, 1676, 2009, 2235
BstV2I	GAAGAC	4 cut(s)	279, 386, 1109, 1244
BstX2I	RGATCY	3 cut(s)	438, 1130, 1195
BstYI	RGATCY	3 cut(s)	438, 1130, 1195
Bsu15I	ATCGAT	2 cut(s)	1566, 2136
BsuRI	GGCC	3 cut(s)	616, 1253, 1922
BsuTUI	ATCGAT	2 cut(s)	1566, 2136
BtgI	CCRYGG	3 cut(s)	695, 1350, 1434
BtsCI	GGATG	7 cut(s)	186, 436, 772, 1369, 1632, 2163, 2212
BtsIMutI	CAGTG	1 cut(s)	2043
Cac8I	GCNNGC	5 cut(s)	479, 614, 1506, 1845, 2073
CaiI	CAGNNNCTG	4 cut(s)	920, 1286, 1301, 1787
CciI	TCATGA	2 cut(s)	1087, 1426
CfoI	GCGC	3 cut(s)	100, 477, 1944
Cfr10I	RCCGGY	2 cut(s)	356, 612
Cfr13I	GGNCC	5 cut(s)	163, 460, 811, 983, 1921
ClaI	ATCGAT	2 cut(s)	1566, 2136
CseI	GACGC	6 cut(s)	299, 456, 1665, 1779, 2015, 2241
Csp6I	GTAC	5 cut(s)	253, 605, 770, 1630, 1741
CviQI	GTAC	5 cut(s)	253, 605, 770, 1630, 1741
DdeI	CTNAG	6 cut(s)	249, 663, 1163, 1554, 1956, 2082
DraIII	CACNNNGTG	1 cut(s)	1697
DriI	GACNNNNNGTC	1 cut(s)	466
EaeI	YGGCCR	2 cut(s)	614, 1251
Eam1104I	CTCTTC	4 cut(s)	1716, 1730, 2089, 2173
Eam1105I	GACNNNNNGTC	1 cut(s)	466
EarI	CTCTTC	4 cut(s)	1716, 1730, 2089, 2173
EciI	GGCGGA	1 cut(s)	135
Ecl136II	GAGCTC	2 cut(s)	2143, 2183
Eco130I	CCWWGG	2 cut(s)	1350, 1434
Eco24I	GRGCYC	2 cut(s)	2145, 2185
Eco31I	GGTCTC	2 cut(s)	117, 194
Eco32I	GATATC	1 cut(s)	1621
Eco47I	GGWCC	4 cut(s)	163, 460, 811, 983
Eco53kI	GAGCTC	2 cut(s)	2143, 2183
Eco57I	CTGAAG	3 cut(s)	1017, 1248, 1754
Eco88I	CYCGRG	1 cut(s)	482
EcoICRI	GAGCTC	2 cut(s)	2143, 2183
EcoO109I	RGGNCCY	2 cut(s)	460, 1921
EcoRI	GAATTC	2 cut(s)	182, 1016
EcoRII	CCWGG	4 cut(s)	127, 616, 667, 838
EcoRV	GATATC	1 cut(s)	1621
EcoT14I	CCWWGG	2 cut(s)	1350, 1434
EcoT38I	GRGCYC	2 cut(s)	2145, 2185
ErhI	CCWWGG	2 cut(s)	1350, 1434
FaqI	GGGAC	4 cut(s)	80, 623, 969, 1859
FauI	CCCGC	2 cut(s)	161, 994
FbaI	TGATCA	2 cut(s)	730, 1305
FblI	GTMKAC	1 cut(s)	264
FokI	GGATG	7 cut(s)	173, 443, 779, 1376, 1639, 2170, 2219
FriOI	GRGCYC	2 cut(s)	2145, 2185
FspBI	CTAG	3 cut(s)	1134, 1491, 1844
GlaI	GCGC	3 cut(s)	99, 476, 1943
GsuI	CTGGAG	2 cut(s)	152, 690
HaeIII	GGCC	3 cut(s)	616, 1253, 1922
HapII	CCGG	9 cut(s)	357, 392, 425, 464, 519, 613, 777, 1247, 1857
HgaI	GACGC	6 cut(s)	299, 456, 1665, 1779, 2015, 2241
HhaI	GCGC	3 cut(s)	100, 477, 1944
Hin1I	GRCGYC	2 cut(s)	291, 467
Hin6I	GCGC	3 cut(s)	98, 475, 1942
HinP1I	GCGC	3 cut(s)	98, 475, 1942
HincII	GTYRAC	1 cut(s)	265
HindII	GTYRAC	1 cut(s)	265
HindIII	AAGCTT	2 cut(s)	193, 882
HinfI	GANTC	4 cut(s)	51, 281, 757, 2165
HpaII	CCGG	9 cut(s)	357, 392, 425, 464, 519, 613, 777, 1247, 1857
HphI	GGTGA	3 cut(s)	1181, 1709, 1873
Hpy166II	GTNNAC	3 cut(s)	265, 811, 1672
Hpy8I	GTNNAC	3 cut(s)	265, 811, 1672
Hpy99I	CGWCG	6 cut(s)	119, 266, 293, 305, 1235, 1795
HpyAV	CCTTC	6 cut(s)	65, 266, 395, 547, 1096, 1334
HpyCH4III	ACNGT	6 cut(s)	587, 815, 1218, 1480, 1634, 1885
HpyCH4IV	ACGT	1 cut(s)	1531
HpyF10VI	GCNNNNNNNGC	6 cut(s)	117, 146, 870, 968, 1250, 1688
HpyF3I	CTNAG	6 cut(s)	249, 663, 1163, 1554, 1956, 2082
HpySE526I	ACGT	1 cut(s)	1531
Hsp92I	GRCGYC	2 cut(s)	291, 467
HspAI	GCGC	3 cut(s)	98, 475, 1942
KpnI	GGTACC	1 cut(s)	608
KroI	GCCGGC	1 cut(s)	612
KroNI	GCCGGC	1 cut(s)	614
Ksp22I	TGATCA	2 cut(s)	730, 1305
LguI	GCTCTTC	1 cut(s)	2173
LmnI	GCTCC	8 cut(s)	74, 315, 911, 1075, 1249, 1346, 1438, 1960
LweI	GCATC	3 cut(s)	882, 958, 1030
MaeI	CTAG	3 cut(s)	1134, 1491, 1844
MaeII	ACGT	1 cut(s)	1531
MbiI	CCGCTC	1 cut(s)	315
MfeI	CAATTG	1 cut(s)	831
MflI	RGATCY	3 cut(s)	438, 1130, 1195
MhlI	GDGCHC	3 cut(s)	490, 2145, 2185
MluI	ACGCGT	2 cut(s)	1674, 2233
MlyI	GAGTC	1 cut(s)	60
MroNI	GCCGGC	1 cut(s)	612
MseI	TTAA	8 cut(s)	17, 851, 1176, 1224, 1485, 1545, 1614, 2067
MslI	CAYNNNNRTG	4 cut(s)	941, 1092, 1968, 2150
MspA1I	CMGCKG	4 cut(s)	146, 989, 1283, 1511
MspCI	CTTAAG	2 cut(s)	1223, 1544
MspI	CCGG	9 cut(s)	357, 392, 425, 464, 519, 613, 777, 1247, 1857
MspR9I	CCNGG	7 cut(s)	129, 393, 464, 520, 618, 669, 840
MunI	CAATTG	1 cut(s)	831
Mva1269I	GAATGC	1 cut(s)	1769
MvaI	CCWGG	4 cut(s)	129, 618, 669, 840
MvnI	CGCG	4 cut(s)	981, 1676, 2009, 2235
MwoI	GCNNNNNNNGC	6 cut(s)	117, 146, 870, 968, 1250, 1688
NaeI	GCCGGC	1 cut(s)	614
NciI	CCSGG	3 cut(s)	393, 464, 520
NcoI	CCATGG	2 cut(s)	1350, 1434
NgoMIV	GCCGGC	1 cut(s)	612
NheI	GCTAGC	1 cut(s)	1843
NlaIV	GGNNCC	9 cut(s)	311, 423, 462, 606, 708, 985, 1197, 1348, 2111
NmeAIII	GCCGAG	1 cut(s)	87
NspI	RCATGY	1 cut(s)	1967
PagI	TCATGA	2 cut(s)	1087, 1426
PciSI	GCTCTTC	1 cut(s)	2173
PcsI	WCGNNNNNNNCGW	2 cut(s)	285, 297
PctI	GAATGC	1 cut(s)	1769
PdiI	GCCGGC	1 cut(s)	614
PfeI	GAWTC	3 cut(s)	281, 757, 2165
PflFI	GACNNNGTC	1 cut(s)	299
PflMI	CCANNNNNTGG	1 cut(s)	623
PfoI	TCCNGGA	2 cut(s)	391, 518
PleI	GAGTC	1 cut(s)	59
PpsI	GAGTC	1 cut(s)	59
PpuMI	RGGWCCY	1 cut(s)	460
PshBI	ATTAAT	1 cut(s)	1176
PsiI	TTATAA	1 cut(s)	1047
Psp124BI	GAGCTC	2 cut(s)	2145, 2185
Psp5II	RGGWCCY	1 cut(s)	460
Psp6I	CCWGG	4 cut(s)	127, 616, 667, 838
PspGI	CCWGG	4 cut(s)	127, 616, 667, 838
PspN4I	GGNNCC	9 cut(s)	311, 423, 462, 606, 708, 985, 1197, 1348, 2111
PspPI	GGNCC	5 cut(s)	163, 460, 811, 983, 1921
PspPPI	RGGWCCY	1 cut(s)	460
PstI	CTGCAG	3 cut(s)	1575, 1806, 1873
PstNI	CAGNNNCTG	4 cut(s)	920, 1286, 1301, 1787
PsuI	RGATCY	3 cut(s)	438, 1130, 1195
PsyI	GACNNNGTC	1 cut(s)	299
PvuII	CAGCTG	3 cut(s)	146, 1283, 1511
RsaI	GTAC	5 cut(s)	254, 606, 771, 1631, 1742
RsaNI	GTAC	5 cut(s)	253, 605, 770, 1630, 1741
RseI	CAYNNNNRTG	4 cut(s)	941, 1092, 1968, 2150
SacI	GAGCTC	2 cut(s)	2145, 2185
SalI	GTCGAC	1 cut(s)	263
SapI	GCTCTTC	1 cut(s)	2173
SaqAI	TTAA	8 cut(s)	17, 851, 1176, 1224, 1485, 1545, 1614, 2067
Sau96I	GGNCC	5 cut(s)	163, 460, 811, 983, 1921
SchI	GAGTC	1 cut(s)	60
ScrFI	CCNGG	7 cut(s)	129, 393, 464, 520, 618, 669, 840
SduI	GDGCHC	3 cut(s)	490, 2145, 2185
SfaNI	GCATC	3 cut(s)	882, 958, 1030
SfcI	CTRYAG	6 cut(s)	223, 528, 1571, 1802, 1869, 1902
SinI	GGWCC	4 cut(s)	163, 460, 811, 983
SmiMI	CAYNNNNRTG	4 cut(s)	941, 1092, 1968, 2150
SmlI	CTYRAG	2 cut(s)	1223, 1544
SmoI	CTYRAG	2 cut(s)	1223, 1544
SspI	AATATT	2 cut(s)	13, 1751
SspMI	CTAG	3 cut(s)	1134, 1491, 1844
SstI	GAGCTC	2 cut(s)	2145, 2185
StyD4I	CCNGG	7 cut(s)	127, 391, 462, 518, 616, 667, 838
StyI	CCWWGG	2 cut(s)	1350, 1434
TaaI	ACNGT	6 cut(s)	587, 815, 1218, 1480, 1634, 1885
TaiI	ACGT	1 cut(s)	1534
TaqII	GACCGA	2 cut(s)	151, 1073
TatI	WGTACW	1 cut(s)	1629
TauI	GCSGC	4 cut(s)	114, 143, 356, 2012
TfiI	GAWTC	3 cut(s)	281, 757, 2165
Tru1I	TTAA	8 cut(s)	17, 851, 1176, 1224, 1485, 1545, 1614, 2067
Tru9I	TTAA	8 cut(s)	17, 851, 1176, 1224, 1485, 1545, 1614, 2067
TscAI	CASTG	1 cut(s)	2050
TspGWI	ACGGA	1 cut(s)	684
TspRI	CASTG	1 cut(s)	2050
Tth111I	GACNNNGTC	1 cut(s)	299
Van91I	CCANNNNNTGG	1 cut(s)	623
Vha464I	CTTAAG	2 cut(s)	1223, 1544
VpaK11BI	GGWCC	4 cut(s)	163, 460, 811, 983
VspI	ATTAAT	1 cut(s)	1176
XapI	RAATTY	4 cut(s)	173, 182, 373, 1016
XbaI	TCTAGA	2 cut(s)	1133, 1490
XceI	RCATGY	1 cut(s)	1967
XmiI	GTMKAC	1 cut(s)	264
XspI	CTAG	3 cut(s)	1134, 1491, 1844

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.