Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0011015

Physical Location & Seq

Genomic Coordinates Forward (+)

10116152 .. 10119369

Gene Structure 3218 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGTTCATCAACGGCAACACCAACGCCAGCCTCAATGCGGCGGAGGACCCGGAATCCATCACCGCCCCGTTAATCTCGCGCCAGCGGTACGCCCCGAACTCCACGTCGCAGGTGGCGCTGGTCGGCGCCAAAGTCCGCCCAATCGAGAGCCTCGATTACGAGATTCTCGAGAACGAGTTCTTCAAGCAGGACTGGCGGAGTTGCGGGAAGGCCCACGTGTTACAGTACATATTCATGAAGTGGGTCTCGTGTTTCCTTGTTGGCATCGTCGTCGGACTCATTGCATTCTGCAACAACCTCGCCGTCGAAAACATCGCCGGCATTAAGTTTGTTGTCACGTCCAACATGATGTTGCAGCGCAGGTTTTGGCTGGCTTTTGGCGTCTTCTTCTTCTCAAATTTGGGTTTGACTTTATTCGCGGTTGTGGTCACCGCTTTCGTGGCGCCCGCCGCCGCAGGGTCCGGTATACCGGAGGTGAAGGCGTACCTGAACGGCGTCGATGCGCCCGGAATATATTCAATAAAGACTTTGCTTGTTAAGATTGTTGGCAGTGTTACTGCAGTGTCGTCCTCTCTTCTTATTGGAAAGGCAGGGCCCATGGTGCATATCGGTGCGTGTGTTGCATCATTGTTGGGTCAGGGAGGATCGAAGAAGCATGGCTTGACATGGAAATGGCTGCGATATTTCAAGAATGATCGCGATCGTAGGGATCTCGTAACGTGTGGAGCAGCTGCTGGAATTGGTGCATCCTTTCGTTCCCCTGTCGGTGGGGTACTCTTTGTTTTTGAAGAAATGGCATCTTGGTGGAGAAGTGCCCTTCTGTGGAGAGCTTTCTTTACAACAGCTGTAATCGCAATTGTGCTTCGAGCTCTGATTGATGTTTGCTACAGCGGAAAATGTGGGCTTTTTGGTACTGGGGGGCTGATAATGTTTGATGTCTATTCAGCAAATATATCATATCACATTACCGATGTGCCTCCGGTGCTTCTCCTTGGGTTTGTAGGCGGCATATTGGGCAGCCTATACAATTCTCTATTAACAAAAGTTATCCGAATATACAATATCATCCATGAGAGAGGTGTCGTTTATAAAATTCTTATAGCTTGTACAGTCTCCATTTTCACATCTTGTCTTCTTTTTGGATTACCGTGGCTTGCAACTTGTCGACCTTGCCCACGTGATGCAGTCGAAGCCTGCCCTACCATTGGACGGTCTGGTAACTACAAGAAGTTTCAATGTCCTGCTGGTCACTATAATGACCTTGCCAGCCTCATATTTAACACGAATGATGATGCTATTAGAAACCTCTTCAGCAAGAATACTGATTCTGAGTTTCAATATTCCTCGATGATCATATTTTTTGTCACCTGTTTTTTCTTGAGTATCTTTAGCTACGGGGTGGTTGTGCCAGCTGGTCTTTTCGTACCTGTTATTGTGACTGGTGCATCTTATGGGCGTTTTTTTGGAATGCTGGTTGGTAAACACTCGAATCTCAATCACGGTCTATATGCTGTGCTGGGTGCTGCTGCTCTTCTTGGTGGAACCATGAGGATGACGGTCTCTCTATGTGTAATTATTCTGGAATTGACCAATAATCTGTTATTGCTGCCATTAATAATGGTGGTTCTTCTTGTTTCAAAGACAACGGCAGATGCTTTCAATAGCAATATATATGATCTTATTATGAAAGCAAAGGGTCTTCCCTATCTGGAAAGTCATGTTGAACCATATATGCGGCAGCTGACAGTAGCAGATGTGGTAACAGGGCCACTTCAACTATTTCAAGGCATTGAGAAGGTTGGTAACATAGTACACGTCCTCCGTACTACAGGGCATAATGGGTTCCCTGTGATTGATGAGCCTCCGCTTTCTGAAACGCCTGTTTTGTTTGGTATAGTCCTCCGTGCCCATCTTATTGAATTGCTGAAGAAGAAAGCATTTGTGTCCAACCCAGTTCTTGCGAGCACCGATTCTTTTAAACTCTTCTCATCAGTGGATTTTGCCAAGAGGGGTTCAGGCAAAGGGGACAAGATAGAAGATATAGAACTAACCGAGGAAGAGATGGAAATGTTCATCGACTTGCATCCCTTTACTAATACTTCACCTTATACTGTTGTGGAAACGATGTCACTGGCAAAGGCTCTTATACTTTTCCGAGAAGTTGGCTTAAGGCACCTTCTTGTGATACCCAAGCTGTCCAATAGAACTCCCGTGGTGGGTATATTGACGAGGCACGACTTCATGCCAGAACATATATTGAGCTTGCACCCAATGCTGGTGAGTAGCAGGTGGAAAAGATTAAGATTCCAGATGCCATCTCCGCCAAAACTCATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005247 GO:0005253 GO:0005254 GO:0005575 GO:0006810 GO:0006811 GO:0006820 GO:0006821 GO:0008150 GO:0008308 GO:0008509 GO:0015075 GO:0015103 GO:0015108 GO:0015267 GO:0015318 GO:0015698 GO:0016020 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098656 GO:0098660 GO:0098661 GO:1902476

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

779

Amino Acids

85.41

Weight (kDa)

9.02

Isoelectric Point (pI)

41.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	137 - 550	1.3e-79	Voltage gated chloride channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011015)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G33280
fragaria_vesca	FvH4_1g02460 FvH4_1g02460 FvH4_1g02460 FvH4_1g02460
malus_domestica	MD02G1021600.v1.1 MD15G1164600.v1.1
prunus_persica	Prupe.7G250100_v2.0.a1
pyrus_communis	pycom02g01900 pycom15g14760
rosa_chinensis	RchiOBHm_Chr2g0087431
rosa_laevigata	RLG00000015868
rosa_multiflora	Rmu_sc0006064.1_g000011
rosa_roxburghii	Rroxscaffold_2G00153540
rosa_rugosa	Rorug01G0475300
rosa_samantha	Rh2AG028700 Rh2BG029000 Rh2CG029000 Rh2DG028800
rosa_wichuraiana	Rw2G002290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1089
AarI	CACCTGC	2 cut(s)	100, 2277
Acc36I	ACCTGC	3 cut(s)	100, 351, 2277
AccB1I	GGYRCC	3 cut(s)	125, 442, 2172
AccI	GTMKAC	2 cut(s)	466, 1165
AccII	CGCG	3 cut(s)	79, 419, 699
AclWI	GGATC	2 cut(s)	652, 717
AcsI	RAATTY	2 cut(s)	397, 1092
AcuI	CTGAAG	2 cut(s)	1294, 1946
AcvI	CACGTG	2 cut(s)	217, 1178
AcyI	GRCGYC	4 cut(s)	126, 381, 443, 495
AfaI	GTAC	9 cut(s)	89, 227, 485, 774, 913, 1108, 1425, 1812, 1825
AfiI	CCNNNNNNNGG	8 cut(s)	37, 456, 767, 822, 1205, 1209, 2216, 2275
AflII	CTTAAG	1 cut(s)	2167
AflIII	ACRYGT	3 cut(s)	216, 719, 1813
AjiI	CACGTC	3 cut(s)	105, 339, 1816
Alw21I	GWGCWC	2 cut(s)	871, 1967
Alw26I	GTCTC	3 cut(s)	250, 1117, 1564
AlwI	GGATC	2 cut(s)	652, 717
AlwNI	CAGNNNCTG	1 cut(s)	734
Ama87I	CYCGRG	1 cut(s)	167
AoxI	GGCC	3 cut(s)	210, 593, 1766
ApaI	GGGCCC	1 cut(s)	597
ApeKI	GCWGC	9 cut(s)	355, 676, 728, 731, 1017, 1523, 1526, 1606, 1738
ApoI	RAATTY	2 cut(s)	397, 1092
ArsI	GACNNNNNNTTYG	2 cut(s)	89, 121
AseI	ATTAAT	1 cut(s)	1613
Asp700I	GAANNNNTTC	2 cut(s)	176, 514
AspLEI	GCGC	6 cut(s)	81, 118, 128, 360, 445, 505
AspS9I	GGNCC	6 cut(s)	46, 211, 459, 593, 594, 1766
AsuC2I	CCSGG	2 cut(s)	50, 507
AsuHPI	GGTGA	6 cut(s)	52, 421, 487, 1357, 2094, 2290
AvaI	CYCGRG	1 cut(s)	167
AvaII	GGWCC	2 cut(s)	46, 459
BaeGI	GKGCMC	3 cut(s)	597, 817, 1909
BanI	GGYRCC	3 cut(s)	125, 442, 2172
BanII	GRGCYC	2 cut(s)	597, 871
BauI	CACGAG	1 cut(s)	247
BbrPI	CACGTG	2 cut(s)	217, 1178
BbsI	GAAGAC	3 cut(s)	376, 1124, 1691
Bbv12I	GWGCWC	2 cut(s)	871, 1967
BbvI	GCAGC	9 cut(s)	367, 663, 718, 740, 1029, 1510, 1513, 1593, 1750
BccI	CCATC	4 cut(s)	65, 1917, 2056, 2323
BceAI	ACGGC	4 cut(s)	28, 287, 508, 1662
BcgI	CGANNNNNNTGC	4 cut(s)	253, 280, 287, 314
BclI	TGATCA	1 cut(s)	1350
BcnI	CCSGG	2 cut(s)	50, 507
BcoDI	GTCTC	3 cut(s)	250, 1117, 1564
BfmI	CTRYAG	3 cut(s)	558, 886, 1827
BfoI	RGCGCY	3 cut(s)	119, 129, 446
BfrI	CTTAAG	1 cut(s)	2167
BfuAI	ACCTGC	3 cut(s)	100, 351, 2277
Bme1390I	CCNGG	2 cut(s)	50, 507
Bme18I	GGWCC	2 cut(s)	46, 459
BmeT110I	CYCGRG	1 cut(s)	167
BmgBI	CACGTC	3 cut(s)	105, 339, 1816
BmgT120I	GGNCC	6 cut(s)	46, 211, 459, 593, 594, 1766
BmiI	GGNNCC	8 cut(s)	48, 127, 444, 460, 595, 1543, 1844, 2174
BmrFI	CCNGG	2 cut(s)	50, 507
BmrI	ACTGGG	2 cut(s)	924, 1946
BmuI	ACTGGG	2 cut(s)	924, 1946
BpiI	GAAGAC	3 cut(s)	376, 1124, 1691
BpuEI	CTTGAG	1 cut(s)	1399
BpuMI	CCSGG	2 cut(s)	50, 507
BsaAI	YACGTR	2 cut(s)	217, 1178
BsaBI	GATNNNNATC	1 cut(s)	699
BsaHI	GRCGYC	4 cut(s)	126, 381, 443, 495
BsaI	GGTCTC	2 cut(s)	250, 1564
BsaJI	CCNNGG	5 cut(s)	597, 991, 1148, 2052, 2211
BsaWI	WCCGGW	3 cut(s)	461, 469, 979
BsaXI	ACNNNNNCTCC	2 cut(s)	1803, 1833
Bsc4I	CCNNNNNNNGG	8 cut(s)	37, 456, 767, 822, 1205, 1209, 2216, 2275
Bse118I	RCCGGY	1 cut(s)	317
Bse1I	ACTGG	5 cut(s)	197, 919, 1444, 1952, 2136
Bse3DI	GCAATG	1 cut(s)	279
Bse8I	GATNNNNATC	1 cut(s)	699
BseDI	CCNNGG	5 cut(s)	597, 991, 1148, 2052, 2211
BseGI	GGATG	4 cut(s)	746, 1065, 1557, 2083
BseJI	GATNNNNATC	1 cut(s)	699
BseLI	CCNNNNNNNGG	8 cut(s)	37, 456, 767, 822, 1205, 1209, 2216, 2275
BseMI	GCAATG	1 cut(s)	279
BseMII	CTCAG	1 cut(s)	1320
BseNI	ACTGG	5 cut(s)	197, 919, 1444, 1952, 2136
BseSI	GKGCMC	3 cut(s)	597, 817, 1909
BseXI	GCAGC	9 cut(s)	367, 663, 718, 740, 1029, 1510, 1513, 1593, 1750
BseYI	CCCAGC	1 cut(s)	1516
Bsh1236I	CGCG	3 cut(s)	79, 419, 699
Bsh1285I	CGRYCG	1 cut(s)	703
BshFI	GGCC	3 cut(s)	212, 595, 1768
BshNI	GGYRCC	3 cut(s)	125, 442, 2172
BsiEI	CGRYCG	1 cut(s)	703
BsiHKAI	GWGCWC	2 cut(s)	871, 1967
BsiHKCI	CYCGRG	1 cut(s)	167
BsiSI	CCGG	6 cut(s)	50, 318, 462, 470, 507, 980
BslFI	GGGAC	1 cut(s)	2039
BslI	CCNNNNNNNGG	8 cut(s)	37, 456, 767, 822, 1205, 1209, 2216, 2275
BsmAI	GTCTC	3 cut(s)	250, 1117, 1564
BsmFI	GGGAC	1 cut(s)	2039
BsmI	GAATGC	2 cut(s)	284, 1473
BsnI	GGCC	3 cut(s)	212, 595, 1768
Bso31I	GGTCTC	2 cut(s)	250, 1564
BsoBI	CYCGRG	1 cut(s)	167
Bsp120I	GGGCCC	1 cut(s)	593
Bsp1286I	GDGCHC	5 cut(s)	597, 817, 871, 1909, 1967
Bsp1407I	TGTACA	1 cut(s)	1106
Bsp143I	GATC	6 cut(s)	644, 694, 700, 709, 1350, 1675
Bsp19I	CCATGG	1 cut(s)	597
Bsp68I	TCGCGA	1 cut(s)	699
BspANI	GGCC	3 cut(s)	212, 595, 1768
BspCNI	CTCAG	1 cut(s)	1321
BspFNI	CGCG	3 cut(s)	79, 419, 699
BspHI	TCATGA	1 cut(s)	234
BspLI	GGNNCC	8 cut(s)	48, 127, 444, 460, 595, 1543, 1844, 2174
BspMAI	CTGCAG	1 cut(s)	562
BspMI	ACCTGC	3 cut(s)	100, 351, 2277
BspPI	GGATC	2 cut(s)	652, 717
BspQI	GCTCTTC	1 cut(s)	1536
BspT107I	GGYRCC	3 cut(s)	125, 442, 2172
BspTI	CTTAAG	1 cut(s)	2167
BspTNI	GGTCTC	2 cut(s)	250, 1564
BsrDI	GCAATG	1 cut(s)	279
BsrFI	RCCGGY	1 cut(s)	317
BsrGI	TGTACA	1 cut(s)	1106
BsrI	ACTGG	5 cut(s)	197, 919, 1444, 1952, 2136
BssAI	RCCGGY	1 cut(s)	317
BssECI	CCNNGG	5 cut(s)	597, 991, 1148, 2052, 2211
BssMI	GATC	6 cut(s)	644, 694, 700, 709, 1350, 1675
BssNAI	GTATAC	1 cut(s)	467
BssNI	GRCGYC	4 cut(s)	126, 381, 443, 495
BssSI	CACGAG	1 cut(s)	247
BssT1I	CCWWGG	2 cut(s)	597, 991
Bst1107I	GTATAC	1 cut(s)	467
Bst2BI	CACGAG	1 cut(s)	247
Bst4CI	ACNGT	8 cut(s)	225, 1111, 1149, 1212, 1502, 1558, 1747, 2113
Bst6I	CTCTTC	5 cut(s)	579, 1313, 1536, 1988, 2052
BstACI	GRCGYC	4 cut(s)	126, 381, 443, 495
BstAFI	CTTAAG	1 cut(s)	2167
BstAPI	GCANNNNNTGC	1 cut(s)	2272
BstAUI	TGTACA	1 cut(s)	1106
BstBAI	YACGTR	2 cut(s)	217, 1178
BstDEI	CTNAG	1 cut(s)	1329
BstDSI	CCRYGG	3 cut(s)	597, 1148, 2211
BstEII	GGTNACC	1 cut(s)	427
BstF5I	GGATG	4 cut(s)	746, 1065, 1557, 2083
BstFNI	CGCG	3 cut(s)	79, 419, 699
BstH2I	RGCGCY	3 cut(s)	119, 129, 446
BstHHI	GCGC	6 cut(s)	81, 118, 128, 360, 445, 505
BstKTI	GATC	6 cut(s)	647, 697, 703, 712, 1353, 1678
BstMAI	GTCTC	3 cut(s)	250, 1117, 1564
BstMBI	GATC	6 cut(s)	644, 694, 700, 709, 1350, 1675
BstMCI	CGRYCG	1 cut(s)	703
BstPI	GGTNACC	1 cut(s)	427
BstSCI	CCNGG	2 cut(s)	48, 505
BstSFI	CTRYAG	3 cut(s)	558, 886, 1827
BstSLI	GKGCMC	3 cut(s)	597, 817, 1909
BstUI	CGCG	3 cut(s)	79, 419, 699
BstV1I	GCAGC	9 cut(s)	367, 663, 718, 740, 1029, 1510, 1513, 1593, 1750
BstV2I	GAAGAC	3 cut(s)	376, 1124, 1691
BstX2I	RGATCY	1 cut(s)	709
BstYI	RGATCY	1 cut(s)	709
BstZ17I	GTATAC	1 cut(s)	467
BsuRI	GGCC	3 cut(s)	212, 595, 1768
BtgI	CCRYGG	3 cut(s)	597, 1148, 2211
BtgZI	GCGATG	1 cut(s)	298
BtrI	CACGTC	3 cut(s)	105, 339, 1816
BtsCI	GGATG	4 cut(s)	746, 1065, 1557, 2083
BtsI	GCAGTG	2 cut(s)	556, 567
BtsIMutI	CAGTG	4 cut(s)	556, 567, 1998, 2129
BtuMI	TCGCGA	1 cut(s)	699
BveI	ACCTGC	3 cut(s)	100, 351, 2277
CaiI	CAGNNNCTG	1 cut(s)	734
CciI	TCATGA	1 cut(s)	234
CfoI	GCGC	6 cut(s)	81, 118, 128, 360, 445, 505
Cfr10I	RCCGGY	1 cut(s)	317
Cfr13I	GGNCC	6 cut(s)	46, 211, 459, 593, 594, 1766
CseI	GACGC	2 cut(s)	370, 484
Csp6I	GTAC	9 cut(s)	88, 226, 484, 773, 912, 1107, 1424, 1811, 1824
CviAII	CATG	9 cut(s)	235, 346, 598, 656, 666, 1070, 1546, 1718, 2242
CviQI	GTAC	9 cut(s)	88, 226, 484, 773, 912, 1107, 1424, 1811, 1824
DdeI	CTNAG	1 cut(s)	1329
DinI	GGCGCC	2 cut(s)	127, 444
DpnI	GATC	6 cut(s)	646, 696, 702, 711, 1352, 1677
DpnII	GATC	6 cut(s)	644, 694, 700, 709, 1350, 1675
DraI	TTTAAA	1 cut(s)	1978
Eam1104I	CTCTTC	5 cut(s)	579, 1313, 1536, 1988, 2052
EarI	CTCTTC	5 cut(s)	579, 1313, 1536, 1988, 2052
EciI	GGCGGA	4 cut(s)	56, 125, 211, 2310
Ecl136II	GAGCTC	1 cut(s)	869
Eco130I	CCWWGG	2 cut(s)	597, 991
Eco24I	GRGCYC	2 cut(s)	597, 871
Eco31I	GGTCTC	2 cut(s)	250, 1564
Eco47I	GGWCC	2 cut(s)	46, 459
Eco53kI	GAGCTC	1 cut(s)	869
Eco57I	CTGAAG	2 cut(s)	1294, 1946
Eco72I	CACGTG	2 cut(s)	217, 1178
Eco88I	CYCGRG	1 cut(s)	167
Eco91I	GGTNACC	1 cut(s)	427
EcoICRI	GAGCTC	1 cut(s)	869
EcoO109I	RGGNCCY	2 cut(s)	46, 593
EcoO65I	GGTNACC	1 cut(s)	427
EcoT14I	CCWWGG	2 cut(s)	597, 991
EcoT38I	GRGCYC	2 cut(s)	597, 871
EgeI	GGCGCC	2 cut(s)	127, 444
EheI	GGCGCC	2 cut(s)	127, 444
ErhI	CCWWGG	2 cut(s)	597, 991
FaeI	CATG	9 cut(s)	238, 349, 601, 659, 669, 1073, 1549, 1721, 2245
FalI	AAGNNNNNCTT	2 cut(s)	644, 676
FaqI	GGGAC	1 cut(s)	2039
FatI	CATG	9 cut(s)	234, 345, 597, 655, 665, 1069, 1545, 1717, 2241
FauI	CCCGC	2 cut(s)	197, 454
FbaI	TGATCA	1 cut(s)	1350
FblI	GTMKAC	2 cut(s)	466, 1165
FokI	GGATG	4 cut(s)	733, 1052, 1564, 2070
FriOI	GRGCYC	2 cut(s)	597, 871
GlaI	GCGC	6 cut(s)	80, 117, 127, 359, 444, 504
GsaI	CCCAGC	1 cut(s)	1520
HaeII	RGCGCY	3 cut(s)	119, 129, 446
HaeIII	GGCC	3 cut(s)	212, 595, 1768
HapII	CCGG	6 cut(s)	50, 318, 462, 470, 507, 980
HgaI	GACGC	2 cut(s)	370, 484
HhaI	GCGC	6 cut(s)	81, 118, 128, 360, 445, 505
Hin1I	GRCGYC	4 cut(s)	126, 381, 443, 495
Hin1II	CATG	9 cut(s)	238, 349, 601, 659, 669, 1073, 1549, 1721, 2245
Hin6I	GCGC	6 cut(s)	79, 116, 126, 358, 443, 503
HinP1I	GCGC	6 cut(s)	79, 116, 126, 358, 443, 503
HincII	GTYRAC	1 cut(s)	1166
HindII	GTYRAC	1 cut(s)	1166
HinfI	GANTC	7 cut(s)	53, 163, 276, 1325, 1489, 1970, 2304
HpaII	CCGG	6 cut(s)	50, 318, 462, 470, 507, 980
HphI	GGTGA	6 cut(s)	52, 421, 487, 1357, 2094, 2290
Hpy166II	GTNNAC	4 cut(s)	467, 1166, 1481, 1813
Hpy188I	TCNGA	6 cut(s)	275, 873, 1052, 1330, 1873, 2156
Hpy8I	GTNNAC	4 cut(s)	467, 1166, 1481, 1813
Hpy99I	CGWCG	5 cut(s)	109, 272, 275, 308, 500
HpyAV	CCTTC	5 cut(s)	202, 472, 827, 1789, 2186
HpyCH4III	ACNGT	8 cut(s)	225, 1111, 1149, 1212, 1502, 1558, 1747, 2113
HpyCH4IV	ACGT	6 cut(s)	104, 216, 338, 719, 1177, 1815
HpyF3I	CTNAG	1 cut(s)	1329
HpySE526I	ACGT	6 cut(s)	104, 216, 338, 719, 1177, 1815
Hsp92I	GRCGYC	4 cut(s)	126, 381, 443, 495
Hsp92II	CATG	9 cut(s)	238, 349, 601, 659, 669, 1073, 1549, 1721, 2245
HspAI	GCGC	6 cut(s)	79, 116, 126, 358, 443, 503
KasI	GGCGCC	2 cut(s)	125, 442
KroI	GCCGGC	1 cut(s)	317
KroNI	GCCGGC	1 cut(s)	319
Ksp22I	TGATCA	1 cut(s)	1350
Kzo9I	GATC	6 cut(s)	644, 694, 700, 709, 1350, 1675
LguI	GCTCTTC	1 cut(s)	1536
LmnI	GCTCC	1 cut(s)	725
Lsp1109I	GCAGC	9 cut(s)	367, 663, 718, 740, 1029, 1510, 1513, 1593, 1750
MaeII	ACGT	6 cut(s)	104, 216, 338, 719, 1177, 1815
MalI	GATC	6 cut(s)	646, 696, 702, 711, 1352, 1677
MboI	GATC	6 cut(s)	644, 694, 700, 709, 1350, 1675
MfeI	CAATTG	1 cut(s)	855
MflI	RGATCY	1 cut(s)	709
MhlI	GDGCHC	5 cut(s)	597, 817, 871, 1909, 1967
MluCI	AATT	8 cut(s)	397, 738, 855, 1027, 1092, 1572, 1583, 1919
Mly113I	GGCGCC	2 cut(s)	126, 443
MlyI	GAGTC	1 cut(s)	270
MmeI	TCCRAC	3 cut(s)	253, 366, 1971
MroNI	GCCGGC	1 cut(s)	317
MroXI	GAANNNNTTC	2 cut(s)	176, 514
MseI	TTAA	9 cut(s)	71, 324, 537, 1037, 1278, 1613, 1977, 2168, 2300
MslI	CAYNNNNRTG	6 cut(s)	233, 609, 670, 802, 1254, 1550
MspA1I	CMGCKG	6 cut(s)	85, 731, 845, 891, 1412, 1741
MspCI	CTTAAG	1 cut(s)	2167
MspI	CCGG	6 cut(s)	50, 318, 462, 470, 507, 980
MspR9I	CCNGG	2 cut(s)	50, 507
MunI	CAATTG	1 cut(s)	855
Mva1269I	GAATGC	2 cut(s)	284, 1473
MvnI	CGCG	3 cut(s)	79, 419, 699
NaeI	GCCGGC	1 cut(s)	319
NarI	GGCGCC	2 cut(s)	126, 443
NciI	CCSGG	2 cut(s)	50, 507
NcoI	CCATGG	1 cut(s)	597
NdeII	GATC	6 cut(s)	644, 694, 700, 709, 1350, 1675
NgoMIV	GCCGGC	1 cut(s)	317
NlaIII	CATG	9 cut(s)	238, 349, 601, 659, 669, 1073, 1549, 1721, 2245
NlaIV	GGNNCC	8 cut(s)	48, 127, 444, 460, 595, 1543, 1844, 2174
NmuCI	GTSAC	6 cut(s)	334, 427, 1247, 1363, 1435, 2127
NruI	TCGCGA	1 cut(s)	699
PaeR7I	CTCGAG	1 cut(s)	167
PagI	TCATGA	1 cut(s)	234
PaqCI	CACCTGC	2 cut(s)	100, 2277
PciSI	GCTCTTC	1 cut(s)	1536
PcsI	WCGNNNNNNNCGW	2 cut(s)	150, 165
PctI	GAATGC	2 cut(s)	284, 1473
PdiI	GCCGGC	1 cut(s)	319
PdmI	GAANNNNTTC	2 cut(s)	176, 514
PfeI	GAWTC	6 cut(s)	53, 163, 1325, 1489, 1970, 2304
Ple19I	CGATCG	1 cut(s)	703
PleI	GAGTC	1 cut(s)	270
PluTI	GGCGCC	2 cut(s)	129, 446
PmaCI	CACGTG	2 cut(s)	217, 1178
PmlI	CACGTG	2 cut(s)	217, 1178
PpsI	GAGTC	1 cut(s)	270
Ppu21I	YACGTR	2 cut(s)	217, 1178
PpuMI	RGGWCCY	1 cut(s)	46
PshBI	ATTAAT	1 cut(s)	1613
PsiI	TTATAA	1 cut(s)	1089
Psp124BI	GAGCTC	1 cut(s)	871
Psp5II	RGGWCCY	1 cut(s)	46
PspCI	CACGTG	2 cut(s)	217, 1178
PspEI	GGTNACC	1 cut(s)	427
PspFI	CCCAGC	1 cut(s)	1516
PspN4I	GGNNCC	8 cut(s)	48, 127, 444, 460, 595, 1543, 1844, 2174
PspOMI	GGGCCC	1 cut(s)	593
PspPI	GGNCC	6 cut(s)	46, 211, 459, 593, 594, 1766
PspPPI	RGGWCCY	1 cut(s)	46
PstI	CTGCAG	1 cut(s)	562
PstNI	CAGNNNCTG	1 cut(s)	734
PsuI	RGATCY	1 cut(s)	709
PvuI	CGATCG	1 cut(s)	703
PvuII	CAGCTG	4 cut(s)	731, 845, 1412, 1741
RruI	TCGCGA	1 cut(s)	699
RsaI	GTAC	9 cut(s)	89, 227, 485, 774, 913, 1108, 1425, 1812, 1825
RsaNI	GTAC	9 cut(s)	88, 226, 484, 773, 912, 1107, 1424, 1811, 1824
RseI	CAYNNNNRTG	6 cut(s)	233, 609, 670, 802, 1254, 1550
SacI	GAGCTC	1 cut(s)	871
SalI	GTCGAC	1 cut(s)	1164
SapI	GCTCTTC	1 cut(s)	1536
SaqAI	TTAA	9 cut(s)	71, 324, 537, 1037, 1278, 1613, 1977, 2168, 2300
Sau3AI	GATC	6 cut(s)	644, 694, 700, 709, 1350, 1675
Sau96I	GGNCC	6 cut(s)	46, 211, 459, 593, 594, 1766
SchI	GAGTC	1 cut(s)	270
ScrFI	CCNGG	2 cut(s)	50, 507
SduI	GDGCHC	5 cut(s)	597, 817, 871, 1909, 1967
SfcI	CTRYAG	3 cut(s)	558, 886, 1827
SfoI	GGCGCC	2 cut(s)	127, 444
Sfr274I	CTCGAG	1 cut(s)	167
SinI	GGWCC	2 cut(s)	46, 459
SlaI	CTCGAG	1 cut(s)	167
SmiMI	CAYNNNNRTG	6 cut(s)	233, 609, 670, 802, 1254, 1550
SmlI	CTYRAG	3 cut(s)	167, 1378, 2167
SmoI	CTYRAG	3 cut(s)	167, 1378, 2167
Sse9I	AATT	8 cut(s)	397, 738, 855, 1027, 1092, 1572, 1583, 1919
SspDI	GGCGCC	2 cut(s)	125, 442
SspI	AATATT	1 cut(s)	1340
SstI	GAGCTC	1 cut(s)	871
StyD4I	CCNGG	2 cut(s)	48, 505
StyI	CCWWGG	2 cut(s)	597, 991
TaaI	ACNGT	8 cut(s)	225, 1111, 1149, 1212, 1502, 1558, 1747, 2113
TaiI	ACGT	6 cut(s)	107, 219, 341, 722, 1180, 1818
TasI	AATT	8 cut(s)	397, 738, 855, 1027, 1092, 1572, 1583, 1919
TatI	WGTACW	3 cut(s)	225, 1106, 1810
TauI	GCSGC	5 cut(s)	41, 452, 455, 1008, 1738
TfiI	GAWTC	6 cut(s)	53, 163, 1325, 1489, 1970, 2304
Tru1I	TTAA	9 cut(s)	71, 324, 537, 1037, 1278, 1613, 1977, 2168, 2300
Tru9I	TTAA	9 cut(s)	71, 324, 537, 1037, 1278, 1613, 1977, 2168, 2300
TscAI	CASTG	4 cut(s)	556, 567, 1998, 2136
TseFI	GTSAC	6 cut(s)	334, 427, 1247, 1363, 1435, 2127
TseI	GCWGC	9 cut(s)	355, 676, 728, 731, 1017, 1523, 1526, 1606, 1738
Tsp45I	GTSAC	6 cut(s)	334, 427, 1247, 1363, 1435, 2127
TspDTI	ATGAA	5 cut(s)	223, 251, 1700, 2062, 2230
TspGWI	ACGGA	2 cut(s)	1811, 1892
TspRI	CASTG	4 cut(s)	556, 567, 1998, 2136
Vha464I	CTTAAG	1 cut(s)	2167
VpaK11BI	GGWCC	2 cut(s)	46, 459
VspI	ATTAAT	1 cut(s)	1613
XapI	RAATTY	2 cut(s)	397, 1092
XcmI	CCANNNNNNNNNTGG	1 cut(s)	109
XhoI	CTCGAG	1 cut(s)	167
XmiI	GTMKAC	2 cut(s)	466, 1165
XmnI	GAANNNNTTC	2 cut(s)	176, 514

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.