Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0006166

Physical Location & Seq

Genomic Coordinates Forward (+)

12314447 .. 12317416

Gene Structure 2970 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g17450.7

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1107 bp

ATGGTTGAGGCCTTGGCGGCTAAGAATTCTGAAATTGAGGCCCTTGCCAGTTCCATGGATGGAATTAAGAAACAGGCTGCCTTATCTGAAGGGAGTCTGGCTTCACTGCAGGCAAACATGGAGTCTATGATGAGAAATAGGGAGCTGACAGAGACAAGGATGATGCAGGCTCTTCGAGAGGAGTTGTCTTCTGTAGAACGTAGAGCAGACGAGGAGCGTTCCGCACATAATGCTACCAAAATGGCTGCCATGGAAAGGGAAGTAGAACTGGAACACAGAGCCCTTGAGGCATCCACAGCCCTTGCAAGGATCCAGAGAATAGCAGATGAGAGGACCGCAAAGGCATCAGAGCTTGACCAGAAGTTGGCATTGCTTGAGGTTGAATGTGCCAATTTAAATCAAGAGCTGCAAGATATGGAAGCTAAAGTTCGGCGTGGGCAAAAGAAGTCACCAGAAGAGACAAATCAAGCAATTCAGGTGCAGGCATGGCAGGAAGAAGTGGACCGTGCACACCAAGGTCAGAGGGATGCAGAGGGCAAGCTTTCTTCTTTGGAGGCCGAAGTCCAAAAAATGAGAGTTGAGATGGCTTCCATGAAGAGGGATGCAGAGCATTACTCGCGCCAGGAACACGTGGAGCTAGAGAAGCGCTATCGTGAACTCACTGATCTACTGTACTACAAGCAAACACAATTAGAAACCATGGCTAGTGAAAAGGCCGCAGCAGAGTTTCACTTGGAAAAAGAAATGAAGCGTATCAAGGAAACACAGGTGGAGGCCGAAAGAAGTAGGGTTTCTCGCCGGGCATCAGCTTCCTGGGAAGAGGACGCTGAAATGAAGGCACTTGAGCCTCTTCCGTTGCATCACCGTCATATGGCTGGGGCAAGCATTCAGTTGCAGAAGGCAGCTAAACTATTAGATTCAGGAGCTGTCAGAGCCACAAGATTTCTCTGGCAGTATCCAACAGCCCGACTTATCTTGCTATTCTACCTGGTATTTGTGCATCTCTTCTTGATGTATTTGCTGCATCGCCTTCAGGCTCAAGCAGAAAATTTTTCGGCAAGAGAAGTTGCAGAGTCTATGGGACTTGGTAACACTAACTTACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000301 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005795 GO:0006810 GO:0006891 GO:0006996 GO:0007030 GO:0008150 GO:0009987 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0031090 GO:0031984 GO:0031985 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048193 GO:0051179 GO:0051234 GO:0071840 GO:0098588 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Golgin_A5

Protein Analysis

369

Amino Acids

42.09

Weight (kDa)

5.79

Isoelectric Point (pI)

46.02

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19950 AT2G19950
fragaria_vesca	FvH4_1g05970 FvH4_1g05970 FvH4_1g05970
malus_domestica	MD02G1064300.v1.1 MD15G1195100.v1.1
prunus_persica	Prupe.7G221400_v2.0.a1 Prupe.7G221400_v2.0.a1 Prupe.7G221400_v2.0.a1 Prupe.7G221400_v2.0.a1
pyrus_communis	pycom02g05060 pycom15g17450
rosa_chinensis	RchiOBHm_Chr2g0091661
rosa_laevigata	RLG00000016241
rosa_multiflora	Rmu_sc0000897.1_g000010 Rmu_sc0036676.1_g000001 Rmu_sc0042722.1_g000001
rosa_roxburghii	Rroxscaffold_2G00149820
rosa_rugosa	Rorug02G0019300 Rorug02G0019400 Rorug02G0019500
rosa_samantha	Rh2AG064500 Rh2BG063700 Rh2CG065400 Rh2DG063800
rosa_wichuraiana	Rw2G005600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	364
AccII	CGCG	1 cut(s)	619
AciI	CCGC	4 cut(s)	17, 222, 336, 717
AclWI	GGATC	2 cut(s)	304, 317
AcsI	RAATTY	2 cut(s)	25, 1048
AcuI	CTGAAG	2 cut(s)	108, 1016
AcvI	CACGTG	1 cut(s)	631
AfaI	GTAC	1 cut(s)	674
AfeI	AGCGCT	1 cut(s)	647
AfiI	CCNNNNNNNGG	5 cut(s)	255, 306, 364, 597, 871
AflIII	ACRYGT	1 cut(s)	628
AgsI	TTSAA	1 cut(s)	383
AjnI	CCWGG	3 cut(s)	621, 812, 987
AluBI	AGCT	9 cut(s)	145, 352, 406, 422, 541, 637, 809, 905, 926
AluI	AGCT	9 cut(s)	145, 352, 406, 422, 541, 637, 809, 905, 926
Alw21I	GWGCWC	1 cut(s)	511
Alw26I	GTCTC	2 cut(s)	146, 452
Alw44I	GTGCAC	1 cut(s)	507
AlwI	GGATC	2 cut(s)	304, 317
AlwNI	CAGNNNCTG	1 cut(s)	926
Aor51HI	AGCGCT	1 cut(s)	647
AoxI	GGCC	5 cut(s)	9, 39, 555, 714, 774
ApaLI	GTGCAC	1 cut(s)	507
ApeKI	GCWGC	6 cut(s)	77, 245, 406, 719, 902, 1021
ApoI	RAATTY	2 cut(s)	25, 1048
ArsI	GACNNNNNNTTYG	2 cut(s)	107, 139
AspLEI	GCGC	2 cut(s)	621, 648
AspS9I	GGNCC	3 cut(s)	40, 333, 502
AsuC2I	CCSGG	1 cut(s)	800
AsuHPI	GGTGA	2 cut(s)	441, 854
AvaII	GGWCC	2 cut(s)	333, 502
BaeGI	GKGCMC	1 cut(s)	511
BamHI	GGATCC	1 cut(s)	309
BanII	GRGCYC	1 cut(s)	283
BbrPI	CACGTG	1 cut(s)	631
BbsI	GAAGAC	1 cut(s)	180
Bbv12I	GWGCWC	1 cut(s)	511
BbvI	GCAGC	6 cut(s)	64, 232, 393, 731, 914, 1008
BccI	CCATC	2 cut(s)	53, 577
BcgI	CGANNNNNNTGC	2 cut(s)	155, 189
BciT130I	CCWGG	3 cut(s)	623, 814, 989
BciVI	GTATCC	1 cut(s)	966
BcnI	CCSGG	1 cut(s)	800
BcoDI	GTCTC	2 cut(s)	146, 452
BfaI	CTAG	2 cut(s)	638, 705
BfmI	CTRYAG	2 cut(s)	107, 192
BfoI	RGCGCY	1 cut(s)	649
BfuI	GTATCC	1 cut(s)	966
BglI	GCCNNNNNGGC	2 cut(s)	17, 287
BisI	GCNGC	8 cut(s)	18, 78, 246, 407, 717, 720, 903, 1022
BlsI	GCNGC	8 cut(s)	19, 79, 247, 408, 718, 721, 904, 1023
Bme1390I	CCNGG	4 cut(s)	623, 800, 814, 989
Bme18I	GGWCC	2 cut(s)	333, 502
BmgT120I	GGNCC	3 cut(s)	40, 333, 502
BmiI	GGNNCC	1 cut(s)	311
BmrFI	CCNGG	4 cut(s)	623, 800, 814, 989
BmsI	GCATC	9 cut(s)	153, 299, 353, 517, 592, 812, 868, 1009, 1033
BpiI	GAAGAC	1 cut(s)	180
BplI	GAGNNNNNCTC	2 cut(s)	599, 631
BpuEI	CTTGAG	4 cut(s)	305, 395, 863, 1023
BpuMI	CCSGG	1 cut(s)	800
BsaAI	YACGTR	1 cut(s)	631
BsaJI	CCNNGG	6 cut(s)	12, 54, 249, 514, 699, 813
Bsc4I	CCNNNNNNNGG	5 cut(s)	255, 306, 364, 597, 871
Bse1I	ACTGG	2 cut(s)	48, 273
Bse3DI	GCAATG	1 cut(s)	368
BseBI	CCWGG	3 cut(s)	623, 814, 989
BseDI	CCNNGG	6 cut(s)	12, 54, 249, 514, 699, 813
BseGI	GGATG	5 cut(s)	64, 165, 290, 532, 607
BseLI	CCNNNNNNNGG	5 cut(s)	255, 306, 364, 597, 871
BseMI	GCAATG	1 cut(s)	368
BseNI	ACTGG	2 cut(s)	48, 273
BseRI	GAGGAG	2 cut(s)	194, 227
BseSI	GKGCMC	1 cut(s)	511
BseXI	GCAGC	6 cut(s)	64, 232, 393, 731, 914, 1008
BseYI	CCCAGC	1 cut(s)	875
BsgI	GTGCAG	1 cut(s)	500
Bsh1236I	CGCG	1 cut(s)	619
BshFI	GGCC	5 cut(s)	11, 41, 557, 716, 776
BsiHKAI	GWGCWC	1 cut(s)	511
BsiSI	CCGG	1 cut(s)	799
BslFI	GGGAC	1 cut(s)	1095
BslI	CCNNNNNNNGG	5 cut(s)	255, 306, 364, 597, 871
BsmAI	GTCTC	2 cut(s)	146, 452
BsmFI	GGGAC	1 cut(s)	1095
BsmI	GAATGC	1 cut(s)	885
BsnI	GGCC	5 cut(s)	11, 41, 557, 716, 776
Bsp1286I	GDGCHC	2 cut(s)	283, 511
Bsp143I	GATC	2 cut(s)	309, 664
Bsp19I	CCATGG	3 cut(s)	54, 249, 699
BspACI	CCGC	4 cut(s)	17, 222, 336, 717
BspANI	GGCC	5 cut(s)	11, 41, 557, 716, 776
BspFNI	CGCG	1 cut(s)	619
BspLI	GGNNCC	1 cut(s)	311
BspMAI	CTGCAG	1 cut(s)	111
BspPI	GGATC	2 cut(s)	304, 317
BspQI	GCTCTTC	1 cut(s)	177
BsrDI	GCAATG	1 cut(s)	368
BsrI	ACTGG	2 cut(s)	48, 273
BssECI	CCNNGG	6 cut(s)	12, 54, 249, 514, 699, 813
BssMI	GATC	2 cut(s)	309, 664
BssT1I	CCWWGG	5 cut(s)	12, 54, 249, 514, 699
Bst2UI	CCWGG	3 cut(s)	623, 814, 989
Bst4CI	ACNGT	3 cut(s)	506, 672, 866
Bst6I	CTCTTC	6 cut(s)	177, 450, 590, 813, 855, 1010
BstAPI	GCANNNNNTGC	1 cut(s)	230
BstBAI	YACGTR	1 cut(s)	631
BstC8I	GCNNGC	5 cut(s)	111, 168, 483, 539, 883
BstDEI	CTNAG	1 cut(s)	21
BstDSI	CCRYGG	3 cut(s)	54, 249, 699
BstF5I	GGATG	5 cut(s)	64, 165, 290, 532, 607
BstFNI	CGCG	1 cut(s)	619
BstH2I	RGCGCY	1 cut(s)	649
BstHHI	GCGC	2 cut(s)	621, 648
BstKTI	GATC	2 cut(s)	312, 667
BstMAI	GTCTC	2 cut(s)	146, 452
BstMBI	GATC	2 cut(s)	309, 664
BstMWI	GCNNNNNNNGC	8 cut(s)	17, 230, 287, 296, 487, 616, 643, 932
BstNI	CCWGG	3 cut(s)	623, 814, 989
BstSCI	CCNGG	4 cut(s)	621, 798, 812, 987
BstSFI	CTRYAG	2 cut(s)	107, 192
BstSLI	GKGCMC	1 cut(s)	511
BstUI	CGCG	1 cut(s)	619
BstV1I	GCAGC	6 cut(s)	64, 232, 393, 731, 914, 1008
BstV2I	GAAGAC	1 cut(s)	180
BstX2I	RGATCY	1 cut(s)	309
BstXI	CCANNNNNNTGG	1 cut(s)	55
BstYI	RGATCY	1 cut(s)	309
BsuI	GTATCC	1 cut(s)	966
BsuRI	GGCC	5 cut(s)	11, 41, 557, 716, 776
BtgI	CCRYGG	3 cut(s)	54, 249, 699
BtgZI	GCGATG	1 cut(s)	1010
BtsCI	GGATG	5 cut(s)	64, 165, 290, 532, 607
BtsI	GCAGTG	1 cut(s)	104
BtsIMutI	CAGTG	2 cut(s)	104, 660
Cac8I	GCNNGC	5 cut(s)	111, 168, 483, 539, 883
CaiI	CAGNNNCTG	1 cut(s)	926
CfoI	GCGC	2 cut(s)	621, 648
Cfr13I	GGNCC	3 cut(s)	40, 333, 502
CseI	GACGC	1 cut(s)	833
CsiI	ACCWGGT	1 cut(s)	987
Csp6I	GTAC	1 cut(s)	673
CspCI	CAANNNNNGTGG	2 cut(s)	283, 318
CviAII	CATG	7 cut(s)	55, 118, 250, 486, 592, 700, 1104
CviQI	GTAC	1 cut(s)	673
DdeI	CTNAG	1 cut(s)	21
DpnI	GATC	2 cut(s)	311, 666
DpnII	GATC	2 cut(s)	309, 664
DraI	TTTAAA	1 cut(s)	396
Eam1104I	CTCTTC	6 cut(s)	177, 450, 590, 813, 855, 1010
EarI	CTCTTC	6 cut(s)	177, 450, 590, 813, 855, 1010
Eco130I	CCWWGG	5 cut(s)	12, 54, 249, 514, 699
Eco147I	AGGCCT	1 cut(s)	11
Eco24I	GRGCYC	1 cut(s)	283
Eco47I	GGWCC	2 cut(s)	333, 502
Eco47III	AGCGCT	1 cut(s)	647
Eco57I	CTGAAG	2 cut(s)	108, 1016
Eco72I	CACGTG	1 cut(s)	631
EcoO109I	RGGNCCY	1 cut(s)	40
EcoRI	GAATTC	1 cut(s)	25
EcoRII	CCWGG	3 cut(s)	621, 812, 987
EcoT14I	CCWWGG	5 cut(s)	12, 54, 249, 514, 699
EcoT38I	GRGCYC	1 cut(s)	283
ErhI	CCWWGG	5 cut(s)	12, 54, 249, 514, 699
FaeI	CATG	7 cut(s)	58, 121, 253, 489, 595, 703, 1107
FaqI	GGGAC	1 cut(s)	1095
FatI	CATG	7 cut(s)	54, 117, 249, 485, 591, 699, 1103
FauNDI	CATATG	1 cut(s)	870
Fnu4HI	GCNGC	8 cut(s)	18, 78, 246, 407, 717, 720, 903, 1022
FokI	GGATG	5 cut(s)	71, 172, 277, 539, 614
FriOI	GRGCYC	1 cut(s)	283
Fsp4HI	GCNGC	8 cut(s)	18, 78, 246, 407, 717, 720, 903, 1022
FspBI	CTAG	2 cut(s)	638, 705
GlaI	GCGC	2 cut(s)	620, 647
GluI	GCNGC	8 cut(s)	18, 78, 246, 407, 717, 720, 903, 1022
GsaI	CCCAGC	1 cut(s)	879
HaeII	RGCGCY	1 cut(s)	649
HaeIII	GGCC	5 cut(s)	11, 41, 557, 716, 776
HapII	CCGG	1 cut(s)	799
HgaI	GACGC	1 cut(s)	833
HhaI	GCGC	2 cut(s)	621, 648
Hin1II	CATG	7 cut(s)	58, 121, 253, 489, 595, 703, 1107
Hin6I	GCGC	2 cut(s)	619, 646
HinP1I	GCGC	2 cut(s)	619, 646
HindIII	AAGCTT	1 cut(s)	539
HinfI	GANTC	4 cut(s)	94, 122, 917, 1073
HpaII	CCGG	1 cut(s)	799
HphI	GGTGA	2 cut(s)	441, 854
Hpy166II	GTNNAC	3 cut(s)	502, 509, 656
Hpy188I	TCNGA	5 cut(s)	31, 88, 349, 522, 932
Hpy188III	TCNNGA	6 cut(s)	176, 313, 401, 653, 921, 1009
Hpy8I	GTNNAC	3 cut(s)	502, 509, 656
HpyAV	CCTTC	4 cut(s)	83, 829, 892, 1040
HpyCH4III	ACNGT	3 cut(s)	506, 672, 866
HpyCH4IV	ACGT	2 cut(s)	199, 630
HpyF10VI	GCNNNNNNNGC	8 cut(s)	17, 230, 287, 296, 487, 616, 643, 932
HpyF3I	CTNAG	1 cut(s)	21
HpySE526I	ACGT	2 cut(s)	199, 630
Hsp92II	CATG	7 cut(s)	58, 121, 253, 489, 595, 703, 1107
HspAI	GCGC	2 cut(s)	619, 646
Kzo9I	GATC	2 cut(s)	309, 664
LguI	GCTCTTC	1 cut(s)	177
LmnI	GCTCC	4 cut(s)	142, 214, 634, 923
Lsp1109I	GCAGC	6 cut(s)	64, 232, 393, 731, 914, 1008
LweI	GCATC	9 cut(s)	153, 299, 353, 517, 592, 812, 868, 1009, 1033
MabI	ACCWGGT	1 cut(s)	987
MaeI	CTAG	2 cut(s)	638, 705
MaeII	ACGT	2 cut(s)	199, 630
MaeIII	GTNAC	2 cut(s)	447, 1088
MalI	GATC	2 cut(s)	311, 666
MboI	GATC	2 cut(s)	309, 664
MboII	GAAGA	9 cut(s)	164, 180, 467, 506, 537, 607, 830, 842, 997
MflI	RGATCY	1 cut(s)	309
MhlI	GDGCHC	2 cut(s)	283, 511
MluCI	AATT	7 cut(s)	25, 33, 63, 391, 471, 689, 1048
MlyI	GAGTC	3 cut(s)	103, 131, 1082
MmeI	TCCRAC	1 cut(s)	983
MseI	TTAA	2 cut(s)	66, 395
MspI	CCGG	1 cut(s)	799
MspR9I	CCNGG	4 cut(s)	623, 800, 814, 989
Mva1269I	GAATGC	1 cut(s)	885
MvaI	CCWGG	3 cut(s)	623, 814, 989
MvnI	CGCG	1 cut(s)	619
MwoI	GCNNNNNNNGC	8 cut(s)	17, 230, 287, 296, 487, 616, 643, 932
NciI	CCSGG	1 cut(s)	800
NcoI	CCATGG	3 cut(s)	54, 249, 699
NdeI	CATATG	1 cut(s)	870
NdeII	GATC	2 cut(s)	309, 664
NlaIII	CATG	7 cut(s)	58, 121, 253, 489, 595, 703, 1107
NlaIV	GGNNCC	1 cut(s)	311
NmuCI	GTSAC	1 cut(s)	447
PceI	AGGCCT	1 cut(s)	11
PciSI	GCTCTTC	1 cut(s)	177
PctI	GAATGC	1 cut(s)	885
PfeI	GAWTC	1 cut(s)	917
PflMI	CCANNNNNTGG	1 cut(s)	364
PkrI	GCNGC	8 cut(s)	19, 79, 247, 408, 718, 721, 904, 1023
PleI	GAGTC	3 cut(s)	102, 130, 1081
PmaCI	CACGTG	1 cut(s)	631
PmlI	CACGTG	1 cut(s)	631
PpsI	GAGTC	3 cut(s)	102, 130, 1081
Ppu21I	YACGTR	1 cut(s)	631
Psp6I	CCWGG	3 cut(s)	621, 812, 987
PspCI	CACGTG	1 cut(s)	631
PspFI	CCCAGC	1 cut(s)	875
PspGI	CCWGG	3 cut(s)	621, 812, 987
PspN4I	GGNNCC	1 cut(s)	311
PspPI	GGNCC	3 cut(s)	40, 333, 502
PstI	CTGCAG	1 cut(s)	111
PstNI	CAGNNNCTG	1 cut(s)	926
PsuI	RGATCY	1 cut(s)	309
RsaI	GTAC	1 cut(s)	674
RsaNI	GTAC	1 cut(s)	673
SapI	GCTCTTC	1 cut(s)	177
SaqAI	TTAA	2 cut(s)	66, 395
SatI	GCNGC	8 cut(s)	18, 78, 246, 407, 717, 720, 903, 1022
Sau3AI	GATC	2 cut(s)	309, 664
Sau96I	GGNCC	3 cut(s)	40, 333, 502
SchI	GAGTC	3 cut(s)	103, 131, 1082
ScrFI	CCNGG	4 cut(s)	623, 800, 814, 989
SduI	GDGCHC	2 cut(s)	283, 511
SexAI	ACCWGGT	1 cut(s)	987
SfaNI	GCATC	9 cut(s)	153, 299, 353, 517, 592, 812, 868, 1009, 1033
SfcI	CTRYAG	2 cut(s)	107, 192
SinI	GGWCC	2 cut(s)	333, 502
SmiI	ATTTAAAT	1 cut(s)	396
SmlI	CTYRAG	4 cut(s)	284, 374, 842, 1038
SmoI	CTYRAG	4 cut(s)	284, 374, 842, 1038
Sse9I	AATT	7 cut(s)	25, 33, 63, 391, 471, 689, 1048
SseBI	AGGCCT	1 cut(s)	11
SsiI	CCGC	4 cut(s)	17, 222, 336, 717
SspMI	CTAG	2 cut(s)	638, 705
StuI	AGGCCT	1 cut(s)	11
StyD4I	CCNGG	4 cut(s)	621, 798, 812, 987
StyI	CCWWGG	5 cut(s)	12, 54, 249, 514, 699
SwaI	ATTTAAAT	1 cut(s)	396
TaaI	ACNGT	3 cut(s)	506, 672, 866
TaiI	ACGT	2 cut(s)	202, 633
TaqI	TCGA	1 cut(s)	175
TasI	AATT	7 cut(s)	25, 33, 63, 391, 471, 689, 1048
TatI	WGTACW	1 cut(s)	672
TauI	GCSGC	2 cut(s)	20, 719
TfiI	GAWTC	1 cut(s)	917
Tru1I	TTAA	2 cut(s)	66, 395
Tru9I	TTAA	2 cut(s)	66, 395
TscAI	CASTG	2 cut(s)	111, 667
TseFI	GTSAC	1 cut(s)	447
TseI	GCWGC	6 cut(s)	77, 245, 406, 719, 902, 1021
Tsp45I	GTSAC	1 cut(s)	447
TspDTI	ATGAA	3 cut(s)	608, 761, 848
TspGWI	ACGGA	1 cut(s)	843
TspRI	CASTG	2 cut(s)	111, 667
Van91I	CCANNNNNTGG	1 cut(s)	364
VneI	GTGCAC	1 cut(s)	507
VpaK11BI	GGWCC	2 cut(s)	333, 502
XapI	RAATTY	2 cut(s)	25, 1048
XspI	CTAG	2 cut(s)	638, 705

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.