pycom15g17450

Golgin candidate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006166
Physical Location & Seq
Forward (+)
12314447 .. 12317416
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1107 bp
ATGGTTGAGGCCTTGGCGGCTAAGAATTCTGAAATTGAGGCCCTTGCCAGTTCCATGGATGGAATTAAGAAACAGGCTGCCTTATCTGAAGGGAGTCTGGCTTCACTGCAGGCAAACATGGAGTCTATGATGAGAAATAGGGAGCTGACAGAGACAAGGATGATGCAGGCTCTTCGAGAGGAGTTGTCTTCTGTAGAACGTAGAGCAGACGAGGAGCGTTCCGCACATAATGCTACCAAAATGGCTGCCATGGAAAGGGAAGTAGAACTGGAACACAGAGCCCTTGAGGCATCCACAGCCCTTGCAAGGATCCAGAGAATAGCAGATGAGAGGACCGCAAAGGCATCAGAGCTTGACCAGAAGTTGGCATTGCTTGAGGTTGAATGTGCCAATTTAAATCAAGAGCTGCAAGATATGGAAGCTAAAGTTCGGCGTGGGCAAAAGAAGTCACCAGAAGAGACAAATCAAGCAATTCAGGTGCAGGCATGGCAGGAAGAAGTGGACCGTGCACACCAAGGTCAGAGGGATGCAGAGGGCAAGCTTTCTTCTTTGGAGGCCGAAGTCCAAAAAATGAGAGTTGAGATGGCTTCCATGAAGAGGGATGCAGAGCATTACTCGCGCCAGGAACACGTGGAGCTAGAGAAGCGCTATCGTGAACTCACTGATCTACTGTACTACAAGCAAACACAATTAGAAACCATGGCTAGTGAAAAGGCCGCAGCAGAGTTTCACTTGGAAAAAGAAATGAAGCGTATCAAGGAAACACAGGTGGAGGCCGAAAGAAGTAGGGTTTCTCGCCGGGCATCAGCTTCCTGGGAAGAGGACGCTGAAATGAAGGCACTTGAGCCTCTTCCGTTGCATCACCGTCATATGGCTGGGGCAAGCATTCAGTTGCAGAAGGCAGCTAAACTATTAGATTCAGGAGCTGTCAGAGCCACAAGATTTCTCTGGCAGTATCCAACAGCCCGACTTATCTTGCTATTCTACCTGGTATTTGTGCATCTCTTCTTGATGTATTTGCTGCATCGCCTTCAGGCTCAAGCAGAAAATTTTTCGGCAAGAGAAGTTGCAGAGTCTATGGGACTTGGTAACACTAACTTACCATGA

Protein Analysis

369

Amino Acids

42.09

Weight (kDa)

5.79

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Golgin_A5 PF09787 89 - 341 1.3e-21 Golgin subfamily A member 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 364
AccII CGCG 1 cut(s) 619
AciI CCGC 4 cut(s) 17, 222, 336, 717
AclWI GGATC 2 cut(s) 304, 317
AcsI RAATTY 2 cut(s) 25, 1048
AcuI CTGAAG 2 cut(s) 108, 1016
AcvI CACGTG 1 cut(s) 631
AfaI GTAC 1 cut(s) 674
AfeI AGCGCT 1 cut(s) 647
AfiI CCNNNNNNNGG 5 cut(s) 255, 306, 364, 597, 871
AflIII ACRYGT 1 cut(s) 628
AgsI TTSAA 1 cut(s) 383
AjnI CCWGG 3 cut(s) 621, 812, 987
AluBI AGCT 9 cut(s) 145, 352, 406, 422, 541, 637, 809, 905, 926
AluI AGCT 9 cut(s) 145, 352, 406, 422, 541, 637, 809, 905, 926
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 2 cut(s) 146, 452
Alw44I GTGCAC 1 cut(s) 507
AlwI GGATC 2 cut(s) 304, 317
AlwNI CAGNNNCTG 1 cut(s) 926
Aor51HI AGCGCT 1 cut(s) 647
AoxI GGCC 5 cut(s) 9, 39, 555, 714, 774
ApaLI GTGCAC 1 cut(s) 507
ApeKI GCWGC 6 cut(s) 77, 245, 406, 719, 902, 1021
ApoI RAATTY 2 cut(s) 25, 1048
ArsI GACNNNNNNTTYG 2 cut(s) 107, 139
AspLEI GCGC 2 cut(s) 621, 648
AspS9I GGNCC 3 cut(s) 40, 333, 502
AsuC2I CCSGG 1 cut(s) 800
AsuHPI GGTGA 2 cut(s) 441, 854
AvaII GGWCC 2 cut(s) 333, 502
BaeGI GKGCMC 1 cut(s) 511
BamHI GGATCC 1 cut(s) 309
BanII GRGCYC 1 cut(s) 283
BbrPI CACGTG 1 cut(s) 631
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 6 cut(s) 64, 232, 393, 731, 914, 1008
BccI CCATC 2 cut(s) 53, 577
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 3 cut(s) 623, 814, 989
BciVI GTATCC 1 cut(s) 966
BcnI CCSGG 1 cut(s) 800
BcoDI GTCTC 2 cut(s) 146, 452
BfaI CTAG 2 cut(s) 638, 705
BfmI CTRYAG 2 cut(s) 107, 192
BfoI RGCGCY 1 cut(s) 649
BfuI GTATCC 1 cut(s) 966
BglI GCCNNNNNGGC 2 cut(s) 17, 287
BisI GCNGC 8 cut(s) 18, 78, 246, 407, 717, 720, 903, 1022
BlsI GCNGC 8 cut(s) 19, 79, 247, 408, 718, 721, 904, 1023
Bme1390I CCNGG 4 cut(s) 623, 800, 814, 989
Bme18I GGWCC 2 cut(s) 333, 502
BmgT120I GGNCC 3 cut(s) 40, 333, 502
BmiI GGNNCC 1 cut(s) 311
BmrFI CCNGG 4 cut(s) 623, 800, 814, 989
BmsI GCATC 9 cut(s) 153, 299, 353, 517, 592, 812, 868, 1009, 1033
BpiI GAAGAC 1 cut(s) 180
BplI GAGNNNNNCTC 2 cut(s) 599, 631
BpuEI CTTGAG 4 cut(s) 305, 395, 863, 1023
BpuMI CCSGG 1 cut(s) 800
BsaAI YACGTR 1 cut(s) 631
BsaJI CCNNGG 6 cut(s) 12, 54, 249, 514, 699, 813
Bsc4I CCNNNNNNNGG 5 cut(s) 255, 306, 364, 597, 871
Bse1I ACTGG 2 cut(s) 48, 273
Bse3DI GCAATG 1 cut(s) 368
BseBI CCWGG 3 cut(s) 623, 814, 989
BseDI CCNNGG 6 cut(s) 12, 54, 249, 514, 699, 813
BseGI GGATG 5 cut(s) 64, 165, 290, 532, 607
BseLI CCNNNNNNNGG 5 cut(s) 255, 306, 364, 597, 871
BseMI GCAATG 1 cut(s) 368
BseNI ACTGG 2 cut(s) 48, 273
BseRI GAGGAG 2 cut(s) 194, 227
BseSI GKGCMC 1 cut(s) 511
BseXI GCAGC 6 cut(s) 64, 232, 393, 731, 914, 1008
BseYI CCCAGC 1 cut(s) 875
BsgI GTGCAG 1 cut(s) 500
Bsh1236I CGCG 1 cut(s) 619
BshFI GGCC 5 cut(s) 11, 41, 557, 716, 776
BsiHKAI GWGCWC 1 cut(s) 511
BsiSI CCGG 1 cut(s) 799
BslFI GGGAC 1 cut(s) 1095
BslI CCNNNNNNNGG 5 cut(s) 255, 306, 364, 597, 871
BsmAI GTCTC 2 cut(s) 146, 452
BsmFI GGGAC 1 cut(s) 1095
BsmI GAATGC 1 cut(s) 885
BsnI GGCC 5 cut(s) 11, 41, 557, 716, 776
Bsp1286I GDGCHC 2 cut(s) 283, 511
Bsp143I GATC 2 cut(s) 309, 664
Bsp19I CCATGG 3 cut(s) 54, 249, 699
BspACI CCGC 4 cut(s) 17, 222, 336, 717
BspANI GGCC 5 cut(s) 11, 41, 557, 716, 776
BspFNI CGCG 1 cut(s) 619
BspLI GGNNCC 1 cut(s) 311
BspMAI CTGCAG 1 cut(s) 111
BspPI GGATC 2 cut(s) 304, 317
BspQI GCTCTTC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 368
BsrI ACTGG 2 cut(s) 48, 273
BssECI CCNNGG 6 cut(s) 12, 54, 249, 514, 699, 813
BssMI GATC 2 cut(s) 309, 664
BssT1I CCWWGG 5 cut(s) 12, 54, 249, 514, 699
Bst2UI CCWGG 3 cut(s) 623, 814, 989
Bst4CI ACNGT 3 cut(s) 506, 672, 866
Bst6I CTCTTC 6 cut(s) 177, 450, 590, 813, 855, 1010
BstAPI GCANNNNNTGC 1 cut(s) 230
BstBAI YACGTR 1 cut(s) 631
BstC8I GCNNGC 5 cut(s) 111, 168, 483, 539, 883
BstDEI CTNAG 1 cut(s) 21
BstDSI CCRYGG 3 cut(s) 54, 249, 699
BstF5I GGATG 5 cut(s) 64, 165, 290, 532, 607
BstFNI CGCG 1 cut(s) 619
BstH2I RGCGCY 1 cut(s) 649
BstHHI GCGC 2 cut(s) 621, 648
BstKTI GATC 2 cut(s) 312, 667
BstMAI GTCTC 2 cut(s) 146, 452
BstMBI GATC 2 cut(s) 309, 664
BstMWI GCNNNNNNNGC 8 cut(s) 17, 230, 287, 296, 487, 616, 643, 932
BstNI CCWGG 3 cut(s) 623, 814, 989
BstSCI CCNGG 4 cut(s) 621, 798, 812, 987
BstSFI CTRYAG 2 cut(s) 107, 192
BstSLI GKGCMC 1 cut(s) 511
BstUI CGCG 1 cut(s) 619
BstV1I GCAGC 6 cut(s) 64, 232, 393, 731, 914, 1008
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 309
BstXI CCANNNNNNTGG 1 cut(s) 55
BstYI RGATCY 1 cut(s) 309
BsuI GTATCC 1 cut(s) 966
BsuRI GGCC 5 cut(s) 11, 41, 557, 716, 776
BtgI CCRYGG 3 cut(s) 54, 249, 699
BtgZI GCGATG 1 cut(s) 1010
BtsCI GGATG 5 cut(s) 64, 165, 290, 532, 607
BtsI GCAGTG 1 cut(s) 104
BtsIMutI CAGTG 2 cut(s) 104, 660
Cac8I GCNNGC 5 cut(s) 111, 168, 483, 539, 883
CaiI CAGNNNCTG 1 cut(s) 926
CfoI GCGC 2 cut(s) 621, 648
Cfr13I GGNCC 3 cut(s) 40, 333, 502
CseI GACGC 1 cut(s) 833
CsiI ACCWGGT 1 cut(s) 987
Csp6I GTAC 1 cut(s) 673
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviAII CATG 7 cut(s) 55, 118, 250, 486, 592, 700, 1104
CviQI GTAC 1 cut(s) 673
DdeI CTNAG 1 cut(s) 21
DpnI GATC 2 cut(s) 311, 666
DpnII GATC 2 cut(s) 309, 664
DraI TTTAAA 1 cut(s) 396
Eam1104I CTCTTC 6 cut(s) 177, 450, 590, 813, 855, 1010
EarI CTCTTC 6 cut(s) 177, 450, 590, 813, 855, 1010
Eco130I CCWWGG 5 cut(s) 12, 54, 249, 514, 699
Eco147I AGGCCT 1 cut(s) 11
Eco24I GRGCYC 1 cut(s) 283
Eco47I GGWCC 2 cut(s) 333, 502
Eco47III AGCGCT 1 cut(s) 647
Eco57I CTGAAG 2 cut(s) 108, 1016
Eco72I CACGTG 1 cut(s) 631
EcoO109I RGGNCCY 1 cut(s) 40
EcoRI GAATTC 1 cut(s) 25
EcoRII CCWGG 3 cut(s) 621, 812, 987
EcoT14I CCWWGG 5 cut(s) 12, 54, 249, 514, 699
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 5 cut(s) 12, 54, 249, 514, 699
FaeI CATG 7 cut(s) 58, 121, 253, 489, 595, 703, 1107
FaqI GGGAC 1 cut(s) 1095
FatI CATG 7 cut(s) 54, 117, 249, 485, 591, 699, 1103
FauNDI CATATG 1 cut(s) 870
Fnu4HI GCNGC 8 cut(s) 18, 78, 246, 407, 717, 720, 903, 1022
FokI GGATG 5 cut(s) 71, 172, 277, 539, 614
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 8 cut(s) 18, 78, 246, 407, 717, 720, 903, 1022
FspBI CTAG 2 cut(s) 638, 705
GlaI GCGC 2 cut(s) 620, 647
GluI GCNGC 8 cut(s) 18, 78, 246, 407, 717, 720, 903, 1022
GsaI CCCAGC 1 cut(s) 879
HaeII RGCGCY 1 cut(s) 649
HaeIII GGCC 5 cut(s) 11, 41, 557, 716, 776
HapII CCGG 1 cut(s) 799
HgaI GACGC 1 cut(s) 833
HhaI GCGC 2 cut(s) 621, 648
Hin1II CATG 7 cut(s) 58, 121, 253, 489, 595, 703, 1107
Hin6I GCGC 2 cut(s) 619, 646
HinP1I GCGC 2 cut(s) 619, 646
HindIII AAGCTT 1 cut(s) 539
HinfI GANTC 4 cut(s) 94, 122, 917, 1073
HpaII CCGG 1 cut(s) 799
HphI GGTGA 2 cut(s) 441, 854
Hpy166II GTNNAC 3 cut(s) 502, 509, 656
Hpy188I TCNGA 5 cut(s) 31, 88, 349, 522, 932
Hpy188III TCNNGA 6 cut(s) 176, 313, 401, 653, 921, 1009
Hpy8I GTNNAC 3 cut(s) 502, 509, 656
HpyAV CCTTC 4 cut(s) 83, 829, 892, 1040
HpyCH4III ACNGT 3 cut(s) 506, 672, 866
HpyCH4IV ACGT 2 cut(s) 199, 630
HpyF10VI GCNNNNNNNGC 8 cut(s) 17, 230, 287, 296, 487, 616, 643, 932
HpyF3I CTNAG 1 cut(s) 21
HpySE526I ACGT 2 cut(s) 199, 630
Hsp92II CATG 7 cut(s) 58, 121, 253, 489, 595, 703, 1107
HspAI GCGC 2 cut(s) 619, 646
Kzo9I GATC 2 cut(s) 309, 664
LguI GCTCTTC 1 cut(s) 177
LmnI GCTCC 4 cut(s) 142, 214, 634, 923
Lsp1109I GCAGC 6 cut(s) 64, 232, 393, 731, 914, 1008
LweI GCATC 9 cut(s) 153, 299, 353, 517, 592, 812, 868, 1009, 1033
MabI ACCWGGT 1 cut(s) 987
MaeI CTAG 2 cut(s) 638, 705
MaeII ACGT 2 cut(s) 199, 630
MaeIII GTNAC 2 cut(s) 447, 1088
MalI GATC 2 cut(s) 311, 666
MboI GATC 2 cut(s) 309, 664
MboII GAAGA 9 cut(s) 164, 180, 467, 506, 537, 607, 830, 842, 997
MflI RGATCY 1 cut(s) 309
MhlI GDGCHC 2 cut(s) 283, 511
MluCI AATT 7 cut(s) 25, 33, 63, 391, 471, 689, 1048
MlyI GAGTC 3 cut(s) 103, 131, 1082
MmeI TCCRAC 1 cut(s) 983
MseI TTAA 2 cut(s) 66, 395
MspI CCGG 1 cut(s) 799
MspR9I CCNGG 4 cut(s) 623, 800, 814, 989
Mva1269I GAATGC 1 cut(s) 885
MvaI CCWGG 3 cut(s) 623, 814, 989
MvnI CGCG 1 cut(s) 619
MwoI GCNNNNNNNGC 8 cut(s) 17, 230, 287, 296, 487, 616, 643, 932
NciI CCSGG 1 cut(s) 800
NcoI CCATGG 3 cut(s) 54, 249, 699
NdeI CATATG 1 cut(s) 870
NdeII GATC 2 cut(s) 309, 664
NlaIII CATG 7 cut(s) 58, 121, 253, 489, 595, 703, 1107
NlaIV GGNNCC 1 cut(s) 311
NmuCI GTSAC 1 cut(s) 447
PceI AGGCCT 1 cut(s) 11
PciSI GCTCTTC 1 cut(s) 177
PctI GAATGC 1 cut(s) 885
PfeI GAWTC 1 cut(s) 917
PflMI CCANNNNNTGG 1 cut(s) 364
PkrI GCNGC 8 cut(s) 19, 79, 247, 408, 718, 721, 904, 1023
PleI GAGTC 3 cut(s) 102, 130, 1081
PmaCI CACGTG 1 cut(s) 631
PmlI CACGTG 1 cut(s) 631
PpsI GAGTC 3 cut(s) 102, 130, 1081
Ppu21I YACGTR 1 cut(s) 631
Psp6I CCWGG 3 cut(s) 621, 812, 987
PspCI CACGTG 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 875
PspGI CCWGG 3 cut(s) 621, 812, 987
PspN4I GGNNCC 1 cut(s) 311
PspPI GGNCC 3 cut(s) 40, 333, 502
PstI CTGCAG 1 cut(s) 111
PstNI CAGNNNCTG 1 cut(s) 926
PsuI RGATCY 1 cut(s) 309
RsaI GTAC 1 cut(s) 674
RsaNI GTAC 1 cut(s) 673
SapI GCTCTTC 1 cut(s) 177
SaqAI TTAA 2 cut(s) 66, 395
SatI GCNGC 8 cut(s) 18, 78, 246, 407, 717, 720, 903, 1022
Sau3AI GATC 2 cut(s) 309, 664
Sau96I GGNCC 3 cut(s) 40, 333, 502
SchI GAGTC 3 cut(s) 103, 131, 1082
ScrFI CCNGG 4 cut(s) 623, 800, 814, 989
SduI GDGCHC 2 cut(s) 283, 511
SexAI ACCWGGT 1 cut(s) 987
SfaNI GCATC 9 cut(s) 153, 299, 353, 517, 592, 812, 868, 1009, 1033
SfcI CTRYAG 2 cut(s) 107, 192
SinI GGWCC 2 cut(s) 333, 502
SmiI ATTTAAAT 1 cut(s) 396
SmlI CTYRAG 4 cut(s) 284, 374, 842, 1038
SmoI CTYRAG 4 cut(s) 284, 374, 842, 1038
Sse9I AATT 7 cut(s) 25, 33, 63, 391, 471, 689, 1048
SseBI AGGCCT 1 cut(s) 11
SsiI CCGC 4 cut(s) 17, 222, 336, 717
SspMI CTAG 2 cut(s) 638, 705
StuI AGGCCT 1 cut(s) 11
StyD4I CCNGG 4 cut(s) 621, 798, 812, 987
StyI CCWWGG 5 cut(s) 12, 54, 249, 514, 699
SwaI ATTTAAAT 1 cut(s) 396
TaaI ACNGT 3 cut(s) 506, 672, 866
TaiI ACGT 2 cut(s) 202, 633
TaqI TCGA 1 cut(s) 175
TasI AATT 7 cut(s) 25, 33, 63, 391, 471, 689, 1048
TatI WGTACW 1 cut(s) 672
TauI GCSGC 2 cut(s) 20, 719
TfiI GAWTC 1 cut(s) 917
Tru1I TTAA 2 cut(s) 66, 395
Tru9I TTAA 2 cut(s) 66, 395
TscAI CASTG 2 cut(s) 111, 667
TseFI GTSAC 1 cut(s) 447
TseI GCWGC 6 cut(s) 77, 245, 406, 719, 902, 1021
Tsp45I GTSAC 1 cut(s) 447
TspDTI ATGAA 3 cut(s) 608, 761, 848
TspGWI ACGGA 1 cut(s) 843
TspRI CASTG 2 cut(s) 111, 667
Van91I CCANNNNNTGG 1 cut(s) 364
VneI GTGCAC 1 cut(s) 507
VpaK11BI GGWCC 2 cut(s) 333, 502
XapI RAATTY 2 cut(s) 25, 1048
XspI CTAG 2 cut(s) 638, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.