pycom15g19740

CAAX amino terminal protease family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002247
Physical Location & Seq
Forward (+)
14160269 .. 14161875
1607 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g19740.1

Sequence Viewer

Length: 1431 bp
ATGCCAGAAGAAAATGATACTGCTGCCAGCTTCAGGATATGCTCCAGAAAGGACTCCGAGAGAAAGTCAGAGGTCCAAAACACAGGGTTGCAGGATGTGGAAAATGGTTCCCTGGATCAGACTAACTCTGATGATCGAGAATTGGTCAATGAGGAGGAAGTCAATCCTGTAGATGAAAAAGGTCAAGTACTACAGACAGCAGAAGTTGTTATGAACATGCTAGATGTAACCATGCCTGATACTCTTACAGAAGAAAAGATGAAGAAGGTCTTAACAGCAGTTGATCAAGGAGACACGCTGATGAAAGCTTTGCAAGATGCAGTGCCGGAAGATGTTCGTGGAAAGCTAACATCTGCTGTTTCTGGAGCTCTGCATACACAAGGAACAAAGCTAAAGTTTGATCAACTCTTGGGTGTTGCTCGGATTCCTGACATGTCTTCAGGTCTAAAGTCAAAGATCGAAGACAAGGTCATGGAAACCTCAAGTTCAGAAGGTGTACAGAAAGAAAATTGTTCGTCGGATCTGTTGAAAAAGGATGGTGATCTGGTGGATAGCTCAATTAACGAACTACCGGATGCAAACAAGCCTCCAGGGGGACTAGAATCAGAGGATCCTCCCGCAGAGGGATCAGAAAAGATTTCAAATCTAGACCAGTCACAATCATTGAGCAGTCAGGAAAGTGATATTTCTGGTTCTGTCGGAAAGGACTCTAGCAAATCAGGAAATGATTCTTCCAAAGAAAAGGCTTCCGAAGATCTTTCCAACAGTGAAAAGAGTTCAAACCTAGATCAGTCTCAATCACTGAGCAGTCAGGAAAGTGATATTACTGGTTCTGTTGGAAAGGAAACTAGGGAATCAGGAAATGACAAGTCTTCCAAGGAAAAGGCTCCTGAAGATCTTTCCAATAGTGAAAAGGGTTCAGAACTAGAAAAAAATCCTAACAATTCCAGCCAGGTTGAAATTGTCGGTGGCACTGAGGAAGCAGTTGTAGAGGAGCAGAGAGACCAAGATGGTAGAAACAATCCGTCAGACACGAAGAAAGAAGAAGAAAACGATATCCAGAAGAAGGATAATAAGAATGTGCAGCCCGTGGCAGACCAAAGTAAGAACTTCAGTGTTTCTGAGGCATTGGATGCATTGACAGGGATGGATGATAACACCCAAATGGCTGTCAACAATATTTTTGGTGTTATAGAAAATATGATAACTCAAATGGGGGAGAGTTCAGAACAGGAAAGTGAAGTAAAAGAAGTTGACTCTGTGGCACAGTCTGAATTTGCAGAGGACCATGTCAATGATGACAATAGTCAAGAAGATTCAGAAGCCAGTAAGACTGACCAAAATGTACAAATGGATACATTAAGCAATGTCCGTGTATCTGATCACCCTGGGAATGGTATGGATTTGCAACCTGATGCACCAAATGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

477

Amino Acids

51.41

Weight (kDa)

4.22

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7750 PF24930 61 - 125 5e-35 Domain of unknown function (DUF7750)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 618
AclWI GGATC 5 cut(s) 123, 528, 605, 618, 634
AcsI RAATTY 1 cut(s) 1274
AcuI CTGAAG 4 cut(s) 16, 423, 912, 1096
AfaI GTAC 3 cut(s) 189, 498, 1347
AfiI CCNNNNNNNGG 5 cut(s) 33, 593, 623, 1066, 1394
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 4 cut(s) 529, 642, 780, 959
AjnI CCWGG 4 cut(s) 111, 589, 951, 1387
AluBI AGCT 6 cut(s) 30, 308, 346, 368, 391, 555
AluI AGCT 6 cut(s) 30, 308, 346, 368, 391, 555
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 3 cut(s) 285, 798, 996
AlwI GGATC 5 cut(s) 123, 528, 605, 618, 634
ApeKI GCWGC 2 cut(s) 23, 1084
ApoI RAATTY 1 cut(s) 1274
ArsI GACNNNNNNTTYG 2 cut(s) 453, 485
Asp700I GAANNNNTTC 2 cut(s) 333, 727
AspS9I GGNCC 2 cut(s) 73, 1285
AsuHPI GGTGA 2 cut(s) 551, 1376
AvaII GGWCC 2 cut(s) 73, 1285
BamHI GGATCC 1 cut(s) 610
BanII GRGCYC 1 cut(s) 370
BbsI GAAGAC 3 cut(s) 429, 468, 864
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 2 cut(s) 10, 1096
BccI CCATC 3 cut(s) 530, 1004, 1141
BciT130I CCWGG 4 cut(s) 113, 591, 953, 1389
BciVI GTATCC 1 cut(s) 1348
BclI TGATCA 3 cut(s) 283, 400, 1381
BcoDI GTCTC 3 cut(s) 285, 798, 996
BfaI CTAG 7 cut(s) 221, 599, 647, 711, 785, 849, 926
BfmI CTRYAG 2 cut(s) 168, 191
BfuI GTATCC 1 cut(s) 1348
BglII AGATCT 2 cut(s) 754, 895
BisI GCNGC 2 cut(s) 24, 1085
BlsI GCNGC 2 cut(s) 25, 1086
BmcAI AGTACT 1 cut(s) 189
Bme1390I CCNGG 4 cut(s) 113, 591, 953, 1389
Bme18I GGWCC 2 cut(s) 73, 1285
BmgT120I GGNCC 2 cut(s) 73, 1285
BmiI GGNNCC 3 cut(s) 109, 612, 888
BmrFI CCNGG 4 cut(s) 113, 591, 953, 1389
BmsI GCATC 4 cut(s) 307, 565, 1123, 1405
BoxI GACNNNNGTC 1 cut(s) 1305
BpiI GAAGAC 3 cut(s) 429, 468, 864
BpmI CTGGAG 3 cut(s) 28, 384, 573
BpuEI CTTGAG 1 cut(s) 466
BsaBI GATNNNNATC 1 cut(s) 540
BsaI GGTCTC 1 cut(s) 996
BsaJI CCNNGG 6 cut(s) 111, 590, 876, 1089, 1387, 1388
BsaWI WCCGGW 1 cut(s) 571
Bsc4I CCNNNNNNNGG 5 cut(s) 33, 593, 623, 1066, 1394
Bse1I ACTGG 3 cut(s) 652, 832, 1326
Bse3DI GCAATG 1 cut(s) 1372
Bse8I GATNNNNATC 1 cut(s) 540
BseBI CCWGG 4 cut(s) 113, 591, 953, 1389
BseDI CCNNGG 6 cut(s) 111, 590, 876, 1089, 1387, 1388
BseGI GGATG 6 cut(s) 100, 541, 580, 1138, 1152, 1156
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 5 cut(s) 33, 593, 623, 1066, 1394
BseMI GCAATG 1 cut(s) 1372
BseMII CTCAG 3 cut(s) 794, 966, 1113
BseNI ACTGG 3 cut(s) 652, 832, 1326
BseRI GAGGAG 2 cut(s) 167, 1007
BseXI GCAGC 2 cut(s) 10, 1096
BsgI GTGCAG 1 cut(s) 1103
BsiHKAI GWGCWC 1 cut(s) 370
BsiSI CCGG 2 cut(s) 326, 572
BslFI GGGAC 1 cut(s) 609
BslI CCNNNNNNNGG 5 cut(s) 33, 593, 623, 1066, 1394
BsmAI GTCTC 3 cut(s) 285, 798, 996
BsmFI GGGAC 1 cut(s) 609
Bso31I GGTCTC 1 cut(s) 996
Bsp1286I GDGCHC 1 cut(s) 370
Bsp1407I TGTACA 2 cut(s) 496, 1345
BspACI CCGC 1 cut(s) 618
BspCNI CTCAG 3 cut(s) 795, 967, 1114
BspLI GGNNCC 3 cut(s) 109, 612, 888
BspPI GGATC 5 cut(s) 123, 528, 605, 618, 634
BspTNI GGTCTC 1 cut(s) 996
BsrDI GCAATG 1 cut(s) 1372
BsrGI TGTACA 2 cut(s) 496, 1345
BsrI ACTGG 3 cut(s) 652, 832, 1326
BssECI CCNNGG 6 cut(s) 111, 590, 876, 1089, 1387, 1388
BssT1I CCWWGG 1 cut(s) 876
Bst2UI CCWGG 4 cut(s) 113, 591, 953, 1389
Bst4CI ACNGT 2 cut(s) 767, 1269
BstAPI GCANNNNNTGC 1 cut(s) 1133
BstAUI TGTACA 2 cut(s) 496, 1345
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 3 cut(s) 803, 975, 1122
BstDSI CCRYGG 1 cut(s) 1089
BstF5I GGATG 6 cut(s) 100, 541, 580, 1138, 1152, 1156
BstMAI GTCTC 3 cut(s) 285, 798, 996
BstMWI GCNNNNNNNGC 1 cut(s) 1133
BstNI CCWGG 4 cut(s) 113, 591, 953, 1389
BstNSI RCATGY 2 cut(s) 220, 436
BstPAI GACNNNNGTC 1 cut(s) 1305
BstSCI CCNGG 4 cut(s) 111, 589, 951, 1387
BstSFI CTRYAG 2 cut(s) 168, 191
BstV1I GCAGC 2 cut(s) 10, 1096
BstV2I GAAGAC 3 cut(s) 429, 468, 864
BstX2I RGATCY 4 cut(s) 520, 610, 754, 895
BstYI RGATCY 4 cut(s) 520, 610, 754, 895
BsuI GTATCC 1 cut(s) 1348
BtgI CCRYGG 1 cut(s) 1089
BtsCI GGATG 6 cut(s) 100, 541, 580, 1138, 1152, 1156
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 5 cut(s) 327, 772, 800, 972, 1120
Cac8I GCNNGC 1 cut(s) 28
Cfr13I GGNCC 2 cut(s) 73, 1285
Csp6I GTAC 3 cut(s) 188, 497, 1346
CviAII CATG 5 cut(s) 217, 232, 433, 472, 1289
CviQI GTAC 3 cut(s) 188, 497, 1346
DdeI CTNAG 3 cut(s) 803, 975, 1122
Ecl136II GAGCTC 1 cut(s) 368
Eco130I CCWWGG 1 cut(s) 876
Eco24I GRGCYC 1 cut(s) 370
Eco31I GGTCTC 1 cut(s) 996
Eco32I GATATC 1 cut(s) 1057
Eco47I GGWCC 2 cut(s) 73, 1285
Eco53kI GAGCTC 1 cut(s) 368
Eco57I CTGAAG 4 cut(s) 16, 423, 912, 1096
EcoICRI GAGCTC 1 cut(s) 368
EcoRII CCWGG 4 cut(s) 111, 589, 951, 1387
EcoRV GATATC 1 cut(s) 1057
EcoT14I CCWWGG 1 cut(s) 876
EcoT22I ATGCAT 1 cut(s) 1138
EcoT38I GRGCYC 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 876
FaeI CATG 5 cut(s) 220, 235, 436, 475, 1292
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 1 cut(s) 609
FatI CATG 5 cut(s) 216, 231, 432, 471, 1288
FauI CCCGC 1 cut(s) 625
FbaI TGATCA 3 cut(s) 283, 400, 1381
Fnu4HI GCNGC 2 cut(s) 24, 1085
FokI GGATG 6 cut(s) 107, 548, 587, 1145, 1159, 1163
FriOI GRGCYC 1 cut(s) 370
Fsp4HI GCNGC 2 cut(s) 24, 1085
FspBI CTAG 7 cut(s) 221, 599, 647, 711, 785, 849, 926
GluI GCNGC 2 cut(s) 24, 1085
GsuI CTGGAG 3 cut(s) 28, 384, 573
HapII CCGG 2 cut(s) 326, 572
Hin1II CATG 5 cut(s) 220, 235, 436, 475, 1292
HincII GTYRAC 2 cut(s) 1174, 1255
HindII GTYRAC 2 cut(s) 1174, 1255
HindIII AAGCTT 1 cut(s) 306
HinfI GANTC 8 cut(s) 53, 424, 602, 707, 728, 854, 1256, 1316
HpaII CCGG 2 cut(s) 326, 572
HphI GGTGA 2 cut(s) 551, 1376
Hpy166II GTNNAC 3 cut(s) 497, 1174, 1255
Hpy8I GTNNAC 3 cut(s) 497, 1174, 1255
Hpy99I CGWCG 1 cut(s) 520
HpyAV CCTTC 3 cut(s) 259, 485, 1060
HpyCH4III ACNGT 2 cut(s) 767, 1269
HpyF10VI GCNNNNNNNGC 1 cut(s) 1133
HpyF3I CTNAG 3 cut(s) 803, 975, 1122
Hsp92II CATG 5 cut(s) 220, 235, 436, 475, 1292
Ksp22I TGATCA 3 cut(s) 283, 400, 1381
LmnI GCTCC 4 cut(s) 47, 365, 892, 994
Lsp1109I GCAGC 2 cut(s) 10, 1096
LweI GCATC 4 cut(s) 307, 565, 1123, 1405
MaeI CTAG 7 cut(s) 221, 599, 647, 711, 785, 849, 926
MaeIII GTNAC 2 cut(s) 226, 654
MflI RGATCY 4 cut(s) 520, 610, 754, 895
MhlI GDGCHC 1 cut(s) 370
MluCI AATT 6 cut(s) 140, 508, 558, 943, 960, 1274
MlyI GAGTC 3 cut(s) 47, 701, 1250
MmeI TCCRAC 4 cut(s) 498, 679, 786, 817
Mph1103I ATGCAT 1 cut(s) 1138
MroXI GAANNNNTTC 2 cut(s) 333, 727
MseI TTAA 3 cut(s) 272, 561, 1361
MslI CAYNNNNRTG 3 cut(s) 299, 1163, 1293
MspI CCGG 2 cut(s) 326, 572
MspR9I CCNGG 4 cut(s) 113, 591, 953, 1389
MvaI CCWGG 4 cut(s) 113, 591, 953, 1389
MwoI GCNNNNNNNGC 1 cut(s) 1133
NlaIII CATG 5 cut(s) 220, 235, 436, 475, 1292
NlaIV GGNNCC 3 cut(s) 109, 612, 888
NmuCI GTSAC 1 cut(s) 654
NsiI ATGCAT 1 cut(s) 1138
NspI RCATGY 2 cut(s) 220, 436
PasI CCCWGGG 1 cut(s) 1388
PciI ACATGT 1 cut(s) 432
PdmI GAANNNNTTC 2 cut(s) 333, 727
PfeI GAWTC 5 cut(s) 424, 602, 728, 854, 1316
PflFI GACNNNGTC 2 cut(s) 467, 1289
PkrI GCNGC 2 cut(s) 25, 1086
PleI GAGTC 3 cut(s) 47, 701, 1250
PpsI GAGTC 3 cut(s) 47, 701, 1250
PscI ACATGT 1 cut(s) 432
PshAI GACNNNNGTC 1 cut(s) 1305
Psp124BI GAGCTC 1 cut(s) 370
Psp6I CCWGG 4 cut(s) 111, 589, 951, 1387
PspGI CCWGG 4 cut(s) 111, 589, 951, 1387
PspN4I GGNNCC 3 cut(s) 109, 612, 888
PspPI GGNCC 2 cut(s) 73, 1285
PsuI RGATCY 4 cut(s) 520, 610, 754, 895
PsyI GACNNNGTC 2 cut(s) 467, 1289
RsaI GTAC 3 cut(s) 189, 498, 1347
RsaNI GTAC 3 cut(s) 188, 497, 1346
RseI CAYNNNNRTG 3 cut(s) 299, 1163, 1293
SacI GAGCTC 1 cut(s) 370
SaqAI TTAA 3 cut(s) 272, 561, 1361
SatI GCNGC 2 cut(s) 24, 1085
Sau96I GGNCC 2 cut(s) 73, 1285
ScaI AGTACT 1 cut(s) 189
SchI GAGTC 3 cut(s) 47, 701, 1250
ScrFI CCNGG 4 cut(s) 113, 591, 953, 1389
SduI GDGCHC 1 cut(s) 370
SfaNI GCATC 4 cut(s) 307, 565, 1123, 1405
SfcI CTRYAG 2 cut(s) 168, 191
SinI GGWCC 2 cut(s) 73, 1285
SmiMI CAYNNNNRTG 3 cut(s) 299, 1163, 1293
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 6 cut(s) 140, 508, 558, 943, 960, 1274
SsiI CCGC 1 cut(s) 618
SspI AATATT 1 cut(s) 1180
SspMI CTAG 7 cut(s) 221, 599, 647, 711, 785, 849, 926
SstI GAGCTC 1 cut(s) 370
StyD4I CCNGG 4 cut(s) 111, 589, 951, 1387
StyI CCWWGG 1 cut(s) 876
TaaI ACNGT 2 cut(s) 767, 1269
TaqI TCGA 2 cut(s) 136, 459
TasI AATT 6 cut(s) 140, 508, 558, 943, 960, 1274
TatI WGTACW 3 cut(s) 187, 496, 1345
TfiI GAWTC 5 cut(s) 424, 602, 728, 854, 1316
Tru1I TTAA 3 cut(s) 272, 561, 1361
Tru9I TTAA 3 cut(s) 272, 561, 1361
TscAI CASTG 5 cut(s) 327, 772, 807, 979, 1120
TseFI GTSAC 1 cut(s) 654
TseI GCWGC 2 cut(s) 23, 1084
Tsp45I GTSAC 1 cut(s) 654
TspDTI ATGAA 4 cut(s) 189, 227, 275, 317
TspGWI ACGGA 2 cut(s) 1014, 1361
TspRI CASTG 5 cut(s) 327, 772, 807, 979, 1120
Tth111I GACNNNGTC 2 cut(s) 467, 1289
VpaK11BI GGWCC 2 cut(s) 73, 1285
XapI RAATTY 1 cut(s) 1274
XbaI TCTAGA 1 cut(s) 646
XceI RCATGY 2 cut(s) 220, 436
XmnI GAANNNNTTC 2 cut(s) 333, 727
XspI CTAG 7 cut(s) 221, 599, 647, 711, 785, 849, 926
ZrmI AGTACT 1 cut(s) 189
Zsp2I ATGCAT 1 cut(s) 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.