pycom15g36900

clathrin assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014227
Physical Location & Seq
Forward (+)
36866980 .. 36869290
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1866 bp
ATGGCTTCAAGCAAATTTAGAAGAGCCTTGGATGCAGTGAAGGACCAAACCAGCATAGGCCTCGCCAAAGTCGGTAGCAGCACCTCTTTGGCGGACCTAGATGTTGCCATTGTGAAAGCAACAACGCACGAAGAATACCCGACCGAGGAGAAATACCTACGAGAAATTCTCAGCCTAACATGCTACTCGCGTGCCTTCATCAGCGCTTGTGTCAACACCATCTCCCGGCGCCTCAACAAGACCAAAAACTGGATCGTGGCTCTCAAGACCCTCATGTTAGTCCAACGACTGCTGTCCGAGGGTGACCCTGCTTATGAACAAGAAATATTTTTCTCCACAAGGCGCGGCAGTCGCTTTCTCAACATGTCTGATTTTCGTGACACCTCGCGCTCCAATTCTTGGGACCATTCTGCGTTTGTGCGCACGTATGCATTGTATCTAGACGAACGGCTTGAGTTCCGAATGCAAAGCCGGCGTGGGAAACGCAGTGCATTTGGGTTTGATGAAGATATTGAAGATCCTCAGGAGGCGGCTAATAATTTTACCAACCAGCAGTCATGTCAGAGAGCCACACCAGTGCATGAAATGAAAGCTGACAAGTTGTTTTCTAGGATGCAGCATTTGCAGCAGCTCCTTGAGCGATTTTTAGGATGTCGCCCAACAGGTTCGATCAGTTCAATCTTTCTTCGCCCAACACTAACAGGCTGCATGCAATATTTCATCCCTGCATGCATGGCCATGGTTGTAATAGTGGCCTTGTACGCGATAGTAAAAGAGAGTTTCCAGATATATTATGACATAACAGAAATAATGGGCATCTTAATCGACCGCTTCATGGAGCTGGAGATCGATCCCGACAGTGCCAAGGTGTACGAGATCTTCTGCCGCATCGGAAAGCAATTTGATGAGTTGGACATGTTCTACGGGTGGTGTAAACAAATCGGCATTGCGCGCTCCTCAGAGTACCCGGAGGTTGAGAAGATTACACCCAAGAAGCTCGAGGTGATGGACGACTACATACGTGACAAGTCGGCTTTGGCACAAGGCAAGAGGTCAGTAAAGGCACTACTAGACCAACAAAAGATTGATGAGGAAAACAAAGATGAAGAATCAAATCATGGCGAAGAAGAAGAAGAAGAAGACATGAACGCCATGAAGGCCCTGCCACCGCCAGAGGGTTTCGTTGAGGAAAGCAATAACAAGGAGATAGAACCAGTAAAGGAAGAGGAAATTAAGAAACCGCAACAAGAAGAAGGCGATCTGTTGAACTTGGGAGAAGATGCTTTGTCGAGCAATGAACATGCTGACAAATTGGCGTTGGCTTTGTTCAACGGCGCCACTGAACAAGCACCAGCAGGTCAACTGGCATGGGAAGCGTTTGCTGACGAAGCTGATTGGGAAACGGCGTTAGTCCAGTCCGCAAGCCACTTGTCGAATCAGAAGGCTTCCCTCGGGGGTGGTTTTGACTTGCTGTTGCTGGACGGAATGTACCAGCAAGCGGAGATATCAGCCGCGATGGCAGGCCCAGGGTATGGAGTTAGTGGCAGTGCGAGTAGTGTTGCATTAGGGTCAGCAGGACGGCCTGCAATGCTAGCATTGCCTGCACCACCAACATCATCGTCTTCGTCAAATCATCCTAGTAAGTCAAATGTACATGTAGACCCTTTTGCTGCTTCGTTAGTGGTACCGCCGCCGTCATATGTGCAAATGTCAGACCTGGAGAAGAAGCAGAAGCTGTTGATGGACGAGCAGTTCATGTGGCAGCAGTATGCAAGGGGTGGGATGCAAGGGCAGGTTGGATTGACAAAGCTACAGCATAACCCTCATCACAACATAAACATGGGAGGTTACACACACACCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

622

Amino Acids

69.6

Weight (kDa)

5.08

Isoelectric Point (pI)

56.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 32 - 223 1.3e-51 ANTH domain
ENTH PF01417 34 - 147 3.7e-07 ENTH domain
ANTH PF07651 247 - 342 9.9e-26 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014227)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03050
fragaria_vesca FvH4_5g30570 FvH4_5g30590
malus_domestica MD08G1222400.v1.1 MD15G1415800.v1.1
prunus_persica Prupe.1G560200_v2.0.a1
pyrus_communis pycom15g36900
rosa_chinensis RchiOBHm_Chr7g0225461
rosa_laevigata RLG00000001817
rosa_multiflora Rmu_sc0000259.1_g000009
rosa_roxburghii Rroxscaffold_3G00233800
rosa_rugosa Rorug07G0229600.1
rosa_samantha Rh7AG373600 Rh7BG364300 Rh7CG391700 Rh7DG375500
rosa_wichuraiana Rw7G031680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 422
Acc36I ACCTGC 2 cut(s) 1346, 1783
Acc65I GGTACC 1 cut(s) 1684
AccB1I GGYRCC 3 cut(s) 228, 1334, 1684
AccB7I CCANNNNNTGG 3 cut(s) 249, 399, 1532
AccI GTMKAC 1 cut(s) 1659
AccII CGCG 6 cut(s) 190, 345, 388, 764, 952, 1514
AclWI GGATC 3 cut(s) 260, 512, 845
AcoI YGGCCR 1 cut(s) 735
AcsI RAATTY 2 cut(s) 14, 165
AcyI GRCGYC 2 cut(s) 229, 1335
AfaI GTAC 6 cut(s) 761, 872, 965, 1490, 1653, 1686
AfeI AGCGCT 1 cut(s) 205
AfiI CCNNNNNNNGG 8 cut(s) 145, 225, 249, 399, 835, 1175, 1498, 1532
AflIII ACRYGT 3 cut(s) 363, 915, 1654
AgsI TTSAA 5 cut(s) 9, 515, 678, 1267, 1330
AjnI CCWGG 2 cut(s) 1525, 1716
AleI CACNNNNGTG 1 cut(s) 575
AloI GAACNNNNNNTCC 2 cut(s) 1736, 1768
AluBI AGCT 7 cut(s) 593, 631, 841, 997, 1391, 1735, 1810
AluI AGCT 7 cut(s) 593, 631, 841, 997, 1391, 1735, 1810
AlwI GGATC 3 cut(s) 260, 512, 845
AlwNI CAGNNNCTG 1 cut(s) 1735
Ama87I CYCGRG 2 cut(s) 998, 1451
Aor51HI AGCGCT 1 cut(s) 205
AoxI GGCC 6 cut(s) 58, 735, 753, 1158, 1522, 1580
ApeKI GCWGC 7 cut(s) 78, 616, 625, 628, 705, 1670, 1762
ApoI RAATTY 2 cut(s) 14, 165
Asp718I GGTACC 1 cut(s) 1684
AspLEI GCGC 8 cut(s) 206, 231, 345, 390, 423, 952, 954, 1337
AspS9I GGNCC 5 cut(s) 43, 94, 403, 1159, 1523
AsuC2I CCSGG 2 cut(s) 226, 968
AsuHPI GGTGA 2 cut(s) 314, 1015
AsuNHI GCTAGC 1 cut(s) 1591
AvaI CYCGRG 2 cut(s) 998, 1451
AvaII GGWCC 3 cut(s) 43, 94, 403
AxyI CCTNAGG 1 cut(s) 522
BalI TGGCCA 1 cut(s) 737
BanI GGYRCC 3 cut(s) 228, 1334, 1684
BarI GAAGNNNNNNTAC 2 cut(s) 863, 895
BbsI GAAGAC 2 cut(s) 1146, 1614
BbvI GCAGC 7 cut(s) 90, 628, 637, 640, 692, 1657, 1774
BccI CCATC 4 cut(s) 227, 1000, 1510, 1735
BceAI ACGGC 5 cut(s) 464, 1348, 1419, 1595, 1678
BcgI CGANNNNNNTGC 4 cut(s) 43, 77, 805, 839
BciT130I CCWGG 2 cut(s) 1527, 1718
BcnI CCSGG 2 cut(s) 226, 968
BfaI CTAG 6 cut(s) 98, 440, 609, 1070, 1592, 1638
BfmI CTRYAG 1 cut(s) 1811
BfoI RGCGCY 3 cut(s) 207, 232, 1338
BfuAI ACCTGC 2 cut(s) 1346, 1783
BglI GCCNNNNNGGC 2 cut(s) 1157, 1517
BglII AGATCT 1 cut(s) 876
Bme1390I CCNGG 4 cut(s) 226, 968, 1527, 1718
Bme18I GGWCC 3 cut(s) 43, 94, 403
BmeT110I CYCGRG 2 cut(s) 998, 1451
BmgT120I GGNCC 5 cut(s) 43, 94, 403, 1159, 1523
BmiI GGNNCC 4 cut(s) 230, 404, 1336, 1686
BmrFI CCNGG 4 cut(s) 226, 968, 1527, 1718
BmsI GCATC 6 cut(s) 22, 603, 825, 897, 1270, 1773
BmtI GCTAGC 1 cut(s) 1595
BoxI GACNNNNGTC 1 cut(s) 292
BpiI GAAGAC 2 cut(s) 1146, 1614
BplI GAGNNNNNCTC 2 cut(s) 153, 185
BpmI CTGGAG 2 cut(s) 863, 1739
BpuEI CTTGAG 3 cut(s) 248, 473, 656
BpuMI CCSGG 2 cut(s) 226, 968
Bsa29I ATCGAT 1 cut(s) 849
BsaAI YACGTR 2 cut(s) 426, 1022
BsaHI GRCGYC 2 cut(s) 229, 1335
BsaJI CCNNGG 8 cut(s) 27, 144, 297, 738, 864, 1450, 1525, 1526
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 8 cut(s) 145, 225, 249, 399, 835, 1175, 1498, 1532
Bse118I RCCGGY 1 cut(s) 471
Bse1I ACTGG 5 cut(s) 254, 575, 1214, 1368, 1414
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 4 cut(s) 945, 1300, 1593, 1595
BseBI CCWGG 2 cut(s) 1527, 1718
BseCI ATCGAT 1 cut(s) 849
BseDI CCNNGG 8 cut(s) 27, 144, 297, 738, 864, 1450, 1525, 1526
BseGI GGATG 6 cut(s) 37, 618, 656, 720, 1633, 1788
BseLI CCNNNNNNNGG 8 cut(s) 145, 225, 249, 399, 835, 1175, 1498, 1532
BseMI GCAATG 4 cut(s) 945, 1300, 1593, 1595
BseMII CTCAG 3 cut(s) 184, 536, 972
BseNI ACTGG 5 cut(s) 254, 575, 1214, 1368, 1414
BsePI GCGCGC 1 cut(s) 950
BseRI GAGGAG 2 cut(s) 161, 946
BseXI GCAGC 7 cut(s) 90, 628, 637, 640, 692, 1657, 1774
BsgI GTGCAG 1 cut(s) 1587
Bsh1236I CGCG 6 cut(s) 190, 345, 388, 764, 952, 1514
Bsh1285I CGRYCG 2 cut(s) 144, 829
BshFI GGCC 6 cut(s) 60, 737, 755, 1160, 1524, 1582
BshNI GGYRCC 3 cut(s) 228, 1334, 1684
BshVI ATCGAT 1 cut(s) 849
BsiEI CGRYCG 2 cut(s) 144, 829
BsiHKCI CYCGRG 2 cut(s) 998, 1451
BsiSI CCGG 3 cut(s) 226, 472, 968
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 8 cut(s) 145, 225, 249, 399, 835, 1175, 1498, 1532
BsmFI GGGAC 1 cut(s) 416
BsmI GAATGC 1 cut(s) 468
BsnI GGCC 6 cut(s) 60, 737, 755, 1160, 1524, 1582
BsoBI CYCGRG 2 cut(s) 998, 1451
Bsp1407I TGTACA 1 cut(s) 1651
Bsp143I GATC 7 cut(s) 252, 517, 669, 846, 850, 876, 1258
Bsp19I CCATGG 1 cut(s) 738
BspANI GGCC 6 cut(s) 60, 737, 755, 1160, 1524, 1582
BspCNI CTCAG 3 cut(s) 183, 535, 971
BspDI ATCGAT 1 cut(s) 849
BspFNI CGCG 6 cut(s) 190, 345, 388, 764, 952, 1514
BspLI GGNNCC 4 cut(s) 230, 404, 1336, 1686
BspMI ACCTGC 2 cut(s) 1346, 1783
BspOI GCTAGC 1 cut(s) 1595
BspPI GGATC 3 cut(s) 260, 512, 845
BspQI GCTCTTC 1 cut(s) 16
BspT107I GGYRCC 3 cut(s) 228, 1334, 1684
BsrDI GCAATG 4 cut(s) 945, 1300, 1593, 1595
BsrFI RCCGGY 1 cut(s) 471
BsrGI TGTACA 1 cut(s) 1651
BsrI ACTGG 5 cut(s) 254, 575, 1214, 1368, 1414
BssAI RCCGGY 1 cut(s) 471
BssECI CCNNGG 8 cut(s) 27, 144, 297, 738, 864, 1450, 1525, 1526
BssHII GCGCGC 1 cut(s) 950
BssMI GATC 7 cut(s) 252, 517, 669, 846, 850, 876, 1258
BssNI GRCGYC 2 cut(s) 229, 1335
BssT1I CCWWGG 3 cut(s) 27, 738, 864
Bst2UI CCWGG 2 cut(s) 1527, 1718
Bst4CI ACNGT 1 cut(s) 860
Bst6I CTCTTC 2 cut(s) 16, 1218
BstACI GRCGYC 2 cut(s) 229, 1335
BstAPI GCANNNNNTGC 2 cut(s) 622, 1601
BstAUI TGTACA 1 cut(s) 1651
BstBAI YACGTR 2 cut(s) 426, 1022
BstDEI CTNAG 3 cut(s) 170, 522, 958
BstDSI CCRYGG 1 cut(s) 738
BstEII GGTNACC 1 cut(s) 302
BstF5I GGATG 6 cut(s) 37, 618, 656, 720, 1633, 1788
BstFNI CGCG 6 cut(s) 190, 345, 388, 764, 952, 1514
BstH2I RGCGCY 3 cut(s) 207, 232, 1338
BstHHI GCGC 8 cut(s) 206, 231, 345, 390, 423, 952, 954, 1337
BstKTI GATC 7 cut(s) 255, 520, 672, 849, 853, 879, 1261
BstMBI GATC 7 cut(s) 252, 517, 669, 846, 850, 876, 1258
BstMCI CGRYCG 2 cut(s) 144, 829
BstNI CCWGG 2 cut(s) 1527, 1718
BstNSI RCATGY 7 cut(s) 183, 367, 712, 732, 919, 1304, 1658
BstPAI GACNNNNGTC 1 cut(s) 292
BstPI GGTNACC 1 cut(s) 302
BstSCI CCNGG 4 cut(s) 224, 966, 1525, 1716
BstSFI CTRYAG 1 cut(s) 1811
BstUI CGCG 6 cut(s) 190, 345, 388, 764, 952, 1514
BstV1I GCAGC 7 cut(s) 90, 628, 637, 640, 692, 1657, 1774
BstV2I GAAGAC 2 cut(s) 1146, 1614
BstX2I RGATCY 2 cut(s) 517, 876
BstYI RGATCY 2 cut(s) 517, 876
Bsu15I ATCGAT 1 cut(s) 849
Bsu36I CCTNAGG 1 cut(s) 522
BsuRI GGCC 6 cut(s) 60, 737, 755, 1160, 1524, 1582
BsuTUI ATCGAT 1 cut(s) 849
BtgI CCRYGG 1 cut(s) 738
BtgZI GCGATG 1 cut(s) 1529
BtsCI GGATG 6 cut(s) 37, 618, 656, 720, 1633, 1788
BtsI GCAGTG 3 cut(s) 42, 493, 1552
BtsIMutI CAGTG 6 cut(s) 42, 493, 582, 865, 1338, 1552
BveI ACCTGC 2 cut(s) 1346, 1783
CaiI CAGNNNCTG 1 cut(s) 1735
CfoI GCGC 8 cut(s) 206, 231, 345, 390, 423, 952, 954, 1337
Cfr10I RCCGGY 1 cut(s) 471
Cfr13I GGNCC 5 cut(s) 43, 94, 403, 1159, 1523
ClaI ATCGAT 1 cut(s) 849
Csp6I GTAC 6 cut(s) 760, 871, 964, 1489, 1652, 1685
CviQI GTAC 6 cut(s) 760, 871, 964, 1489, 1652, 1685
DdeI CTNAG 3 cut(s) 170, 522, 958
DinI GGCGCC 2 cut(s) 230, 1336
DpnI GATC 7 cut(s) 254, 519, 671, 848, 852, 878, 1260
DpnII GATC 7 cut(s) 252, 517, 669, 846, 850, 876, 1258
EaeI YGGCCR 1 cut(s) 735
Eam1104I CTCTTC 2 cut(s) 16, 1218
EarI CTCTTC 2 cut(s) 16, 1218
EciI GGCGGA 1 cut(s) 107
Eco130I CCWWGG 3 cut(s) 27, 738, 864
Eco147I AGGCCT 1 cut(s) 60
Eco32I GATATC 1 cut(s) 1506
Eco47I GGWCC 3 cut(s) 43, 94, 403
Eco47III AGCGCT 1 cut(s) 205
Eco81I CCTNAGG 1 cut(s) 522
Eco88I CYCGRG 2 cut(s) 998, 1451
Eco91I GGTNACC 1 cut(s) 302
EcoO109I RGGNCCY 1 cut(s) 1159
EcoO65I GGTNACC 1 cut(s) 302
EcoRII CCWGG 2 cut(s) 1525, 1716
EcoRV GATATC 1 cut(s) 1506
EcoT14I CCWWGG 3 cut(s) 27, 738, 864
EcoT22I ATGCAT 2 cut(s) 433, 734
EgeI GGCGCC 2 cut(s) 230, 1336
EheI GGCGCC 2 cut(s) 230, 1336
ErhI CCWWGG 3 cut(s) 27, 738, 864
FaqI GGGAC 1 cut(s) 416
FauNDI CATATG 1 cut(s) 1699
FblI GTMKAC 1 cut(s) 1659
FokI GGATG 6 cut(s) 44, 625, 663, 707, 1620, 1795
FspAI RTGCGCAY 1 cut(s) 422
FspBI CTAG 6 cut(s) 98, 440, 609, 1070, 1592, 1638
FspI TGCGCA 1 cut(s) 422
GlaI GCGC 8 cut(s) 205, 230, 344, 389, 422, 951, 953, 1336
GsuI CTGGAG 2 cut(s) 863, 1739
HaeII RGCGCY 3 cut(s) 207, 232, 1338
HaeIII GGCC 6 cut(s) 60, 737, 755, 1160, 1524, 1582
HapII CCGG 3 cut(s) 226, 472, 968
HhaI GCGC 8 cut(s) 206, 231, 345, 390, 423, 952, 954, 1337
Hin1I GRCGYC 2 cut(s) 229, 1335
Hin6I GCGC 8 cut(s) 204, 229, 343, 388, 421, 950, 952, 1335
HinP1I GCGC 8 cut(s) 204, 229, 343, 388, 421, 950, 952, 1335
HincII GTYRAC 2 cut(s) 214, 1361
HindII GTYRAC 2 cut(s) 214, 1361
HinfI GANTC 2 cut(s) 1109, 1435
HpaII CCGG 3 cut(s) 226, 472, 968
HphI GGTGA 2 cut(s) 314, 1015
Hpy166II GTNNAC 5 cut(s) 214, 871, 935, 1361, 1660
Hpy188I TCNGA 8 cut(s) 298, 370, 461, 564, 893, 961, 1440, 1714
Hpy188III TCNNGA 6 cut(s) 265, 377, 440, 524, 784, 854
Hpy8I GTNNAC 5 cut(s) 214, 871, 935, 1361, 1660
HpyAV CCTTC 5 cut(s) 34, 205, 1150, 1247, 1435
HpyCH4III ACNGT 1 cut(s) 860
HpyCH4IV ACGT 2 cut(s) 425, 1021
HpyF3I CTNAG 3 cut(s) 170, 522, 958
HpySE526I ACGT 2 cut(s) 425, 1021
Hsp92I GRCGYC 2 cut(s) 229, 1335
HspAI GCGC 8 cut(s) 204, 229, 343, 388, 421, 950, 952, 1335
KasI GGCGCC 2 cut(s) 228, 1334
KpnI GGTACC 1 cut(s) 1688
KroI GCCGGC 1 cut(s) 471
KroNI GCCGGC 1 cut(s) 473
Kzo9I GATC 7 cut(s) 252, 517, 669, 846, 850, 876, 1258
LguI GCTCTTC 1 cut(s) 16
LmnI GCTCC 4 cut(s) 395, 636, 838, 959
Lsp1109I GCAGC 7 cut(s) 90, 628, 637, 640, 692, 1657, 1774
LweI GCATC 6 cut(s) 22, 603, 825, 897, 1270, 1773
MaeI CTAG 6 cut(s) 98, 440, 609, 1070, 1592, 1638
MaeII ACGT 2 cut(s) 425, 1021
MaeIII GTNAC 4 cut(s) 302, 377, 1022, 1847
MalI GATC 7 cut(s) 254, 519, 671, 848, 852, 878, 1260
MboI GATC 7 cut(s) 252, 517, 669, 846, 850, 876, 1258
MflI RGATCY 2 cut(s) 517, 876
MlsI TGGCCA 1 cut(s) 737
MluCI AATT 7 cut(s) 14, 165, 394, 538, 899, 1230, 1310
MluNI TGGCCA 1 cut(s) 737
Mly113I GGCGCC 2 cut(s) 229, 1335
MmeI TCCRAC 3 cut(s) 307, 891, 1777
Mox20I TGGCCA 1 cut(s) 737
Mph1103I ATGCAT 2 cut(s) 433, 734
MroNI GCCGGC 1 cut(s) 471
MscI TGGCCA 1 cut(s) 737
MseI TTAA 2 cut(s) 821, 1233
MslI CAYNNNNRTG 3 cut(s) 575, 737, 1838
Msp20I TGGCCA 1 cut(s) 737
MspI CCGG 3 cut(s) 226, 472, 968
MspR9I CCNGG 4 cut(s) 226, 968, 1527, 1718
Mva1269I GAATGC 1 cut(s) 468
MvaI CCWGG 2 cut(s) 1527, 1718
MvnI CGCG 6 cut(s) 190, 345, 388, 764, 952, 1514
NaeI GCCGGC 1 cut(s) 473
NarI GGCGCC 2 cut(s) 229, 1335
NciI CCSGG 2 cut(s) 226, 968
NcoI CCATGG 1 cut(s) 738
NdeI CATATG 1 cut(s) 1699
NdeII GATC 7 cut(s) 252, 517, 669, 846, 850, 876, 1258
NgoMIV GCCGGC 1 cut(s) 471
NheI GCTAGC 1 cut(s) 1591
NlaIV GGNNCC 4 cut(s) 230, 404, 1336, 1686
NmuCI GTSAC 3 cut(s) 302, 377, 1022
NsbI TGCGCA 1 cut(s) 422
NsiI ATGCAT 2 cut(s) 433, 734
NspI RCATGY 7 cut(s) 183, 367, 712, 732, 919, 1304, 1658
OliI CACNNNNGTG 1 cut(s) 575
PaeI GCATGC 2 cut(s) 712, 732
PaeR7I CTCGAG 1 cut(s) 998
PasI CCCWGGG 1 cut(s) 1526
PauI GCGCGC 1 cut(s) 950
PceI AGGCCT 1 cut(s) 60
PciI ACATGT 3 cut(s) 363, 915, 1654
PciSI GCTCTTC 1 cut(s) 16
PctI GAATGC 1 cut(s) 468
PdiI GCCGGC 1 cut(s) 473
PfeI GAWTC 2 cut(s) 1109, 1435
PflMI CCANNNNNTGG 3 cut(s) 249, 399, 1532
PluTI GGCGCC 2 cut(s) 232, 1338
Ppu21I YACGTR 2 cut(s) 426, 1022
PscI ACATGT 3 cut(s) 363, 915, 1654
PshAI GACNNNNGTC 1 cut(s) 292
Psp6I CCWGG 2 cut(s) 1525, 1716
PspEI GGTNACC 1 cut(s) 302
PspGI CCWGG 2 cut(s) 1525, 1716
PspN4I GGNNCC 4 cut(s) 230, 404, 1336, 1686
PspPI GGNCC 5 cut(s) 43, 94, 403, 1159, 1523
PspXI VCTCGAGB 1 cut(s) 998
PstNI CAGNNNCTG 1 cut(s) 1735
PsuI RGATCY 2 cut(s) 517, 876
PteI GCGCGC 1 cut(s) 950
RsaI GTAC 6 cut(s) 761, 872, 965, 1490, 1653, 1686
RsaNI GTAC 6 cut(s) 760, 871, 964, 1489, 1652, 1685
RseI CAYNNNNRTG 3 cut(s) 575, 737, 1838
SapI GCTCTTC 1 cut(s) 16
SaqAI TTAA 2 cut(s) 821, 1233
Sau3AI GATC 7 cut(s) 252, 517, 669, 846, 850, 876, 1258
Sau96I GGNCC 5 cut(s) 43, 94, 403, 1159, 1523
ScrFI CCNGG 4 cut(s) 226, 968, 1527, 1718
SfaNI GCATC 6 cut(s) 22, 603, 825, 897, 1270, 1773
SfcI CTRYAG 1 cut(s) 1811
SfoI GGCGCC 2 cut(s) 230, 1336
Sfr274I CTCGAG 1 cut(s) 998
SinI GGWCC 3 cut(s) 43, 94, 403
SlaI CTCGAG 1 cut(s) 998
SmiMI CAYNNNNRTG 3 cut(s) 575, 737, 1838
SmlI CTYRAG 4 cut(s) 263, 452, 635, 998
SmoI CTYRAG 4 cut(s) 263, 452, 635, 998
SphI GCATGC 2 cut(s) 712, 732
Sse9I AATT 7 cut(s) 14, 165, 394, 538, 899, 1230, 1310
SseBI AGGCCT 1 cut(s) 60
SspDI GGCGCC 2 cut(s) 228, 1334
SspI AATATT 2 cut(s) 327, 716
SspMI CTAG 6 cut(s) 98, 440, 609, 1070, 1592, 1638
StuI AGGCCT 1 cut(s) 60
StyD4I CCNGG 4 cut(s) 224, 966, 1525, 1716
StyI CCWWGG 3 cut(s) 27, 738, 864
TaaI ACNGT 1 cut(s) 860
TaiI ACGT 2 cut(s) 428, 1024
TaqI TCGA 6 cut(s) 668, 825, 849, 999, 1289, 1433
TaqII GACCGA 1 cut(s) 158
TasI AATT 7 cut(s) 14, 165, 394, 538, 899, 1230, 1310
TatI WGTACW 1 cut(s) 1651
TauI GCSGC 5 cut(s) 348, 533, 888, 1514, 1693
TfiI GAWTC 2 cut(s) 1109, 1435
Tru1I TTAA 2 cut(s) 821, 1233
Tru9I TTAA 2 cut(s) 821, 1233
TscAI CASTG 6 cut(s) 42, 493, 582, 865, 1345, 1552
TseFI GTSAC 3 cut(s) 302, 377, 1022
TseI GCWGC 7 cut(s) 78, 616, 625, 628, 705, 1670, 1762
Tsp45I GTSAC 3 cut(s) 302, 377, 1022
TspGWI ACGGA 1 cut(s) 1497
TspRI CASTG 6 cut(s) 42, 493, 582, 865, 1345, 1552
Van91I CCANNNNNTGG 3 cut(s) 249, 399, 1532
VpaK11BI GGWCC 3 cut(s) 43, 94, 403
XapI RAATTY 2 cut(s) 14, 165
XbaI TCTAGA 1 cut(s) 439
XceI RCATGY 7 cut(s) 183, 367, 712, 732, 919, 1304, 1658
XhoI CTCGAG 1 cut(s) 998
XmiI GTMKAC 1 cut(s) 1659
XspI CTAG 6 cut(s) 98, 440, 609, 1070, 1592, 1638
Zsp2I ATGCAT 2 cut(s) 433, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.