Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0003026

Physical Location & Seq

Genomic Coordinates Reverse (-)

2991003 .. 2994421

Gene Structure 3419 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGGAAGACCACGCTGTTTGTGATATCAATGTTGGCAGTCCAGAGCTTGAGATAAACCACATCCATGAACGGGTGCCATCTAACGAATCTGGCGTGCAAGAGTTAGAGAGTTCCATGAAAGTTTTGACAAAGGTGGACTTGGACTTGGCATATTCTTCTGAGAAGTTAGCAAATCTCCATGTACCTCTGATGTACCTTTTGGCTCAGCAAAATGATCTCGAGGCAATGGCCACCACGAATAACTATATTCTGGCCGACTTTGTTGAGAAAGTATTGGTGTTCAATCTCCTGTCTAGCATTTTAGATTCTGAGGTACGAGAGCTGGACAATTACATGGATAATCTCCGAGTGGAAAGTGTTGATGCGCGTCAAAAAATATCTTCGTGTAGACACTTGGGAGAACTGTATTCTATAATGAAAGGAAAGCTAAACGATTCTGAAGAATCACTAAAGCAATCTCGGCAACAGATTTCCGAGGTCAAGATGCAGTCTGCAAGGTTTCAGAGAACTGTTCTAGCTTTTGCACATGAAAATTGGAAAAATGACATTGCCATGGATTTGTCAGAAAATTCTCAAATGTCGAATAAAAATGCAATGTCAAATTTAAGGATGCCTAGACAACAGAGACATAATCTATGGATGCTGGAGAAGTCGCTTTCGAGGGAGCTATATCTTGAGAAGAAATTAGCCGAATCAAGACAGAATGAAGAAGAACTTAAATTGAAGATGCATCATATGGAAGAGGTAGCATTTCGCATGGAAGAAGCAGCAGAAGTTGTTTGGGGAAGGTTTTTAGAGGCAGAAAATGCTGCTGAGGTTCTCCAGGGGATTTCAAAGGAGTTATTGGGTCGACTGCAGCTTGTTCATTTTAATCTGAATGGTTCAATTCAGCGAGAAAGCGAGTTAAAATTCAATCTTCAAGCTAGTTTAGAAGAGATTAGAGCAAAAGATAATGCTTTGCATAAGCTCGAGAAGATAATGAAAGAGAACATTAAGAGAGATGTTGAACTGGCTGCTTTGAGAGAAAAGGTGACGCTCCTTGAGAAACAGCTGAAAGAGTCACAGCTCCAGCTGAAGAATGCCAATGCCACGAATGAATCGAGTGAAGAGAAACTTGGGGAAATGGAAAGTTTAGTTGCGTCTTTGAAAGAAAATATCTTTGTAGCAGAAATTAAGGCCGAGAGTGCAGAACTGAAGGTTGCGGAAGTAACAGGAACAAATGTGGAACTCACCGAAGAGCTGAATTTTCTCAAAGGAAGTGCTACTGGCACAGAAAAAAAGGTTGGAAGTCTCGAGAAGCAGCTGAGGGAAGTAGAGATACAATTACAGCATGCAAAAGCATCCTCCGAAGCAAGTCAAGAGCAGCAAAATATGTTATATGCTGCAATTTGGGATATGGAAACTTTGATTGAAGATCTAAAATCGAAGGTTTCCAAAGCGGAGAATAAGACAGAAAGTACTGAGGAGCAATGTATAATGTTGTCTGAAACTAATGCAGAACTAAATGTGGAAATAACTGTTTTAAGGGATAGAGTGGAATTCTTGGAATTGTCTTTGGCTCAAACTAACAATGCAAAACTTGCAAGTGCGGAAGAACTCAATTCCAGAATCAAGCTCATTATGGATATGGCAATGCAACTGGCTGTAGAAAGGGAGCGCATCCAAAAGCAGATGCAAGTCTTAACAATGGAGAAAAAATTTCTGGTAGGGAAATTATGGAACACCAAAAAAGATGCAACTGAGTGCAACAACGAAGACATTGACAACAAAGAGCTTCCACCTTTCAAGTGTACTTCTCCAAATGCTACTTCTATAAAAACATCCGTGGAAGATGTGACAGATTCTTTAGATAAAAGTTTCCAGGTGGATGAACCGTCAGCAGAGACACCTGGCTGTGATAATGATGCAGGGGCTTCCAGTTCTGTATATAGTCCCACTGGCTCGGATTCAGAACTTGACACTGTCGAAGTGGTACAGGCCAAGTGCCTGAGCCTGAAGTACGTTCTCATAGTAATTGTTGTCGTATCAGTCATATATTTGCTTAACCAGGAACCTTTCCCCGTTGAAGATTTTGGCTTCAACTTGGAGTTGGGGAGGAAGGTTGCCACCATCGCATATGCATTCACCATTAACGCAGCGCCAGGGAAATCCTTCGCACACACTGCTGCAGTCTATCATCCCAGGACACCGGAGTTTTCCCCTTGCTTCAGCTCCCGGCATGTTCCACATTGCCATGCCTGTGCAAATTACAAACCAGTTTATACCAATGTGTCACAGTGTGTGTGTGTGTGTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0008360 GO:0022603 GO:0022604 GO:0032878 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0065007 GO:0065008 GO:2000769

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

766

Amino Acids

86.34

Weight (kDa)

5.04

Isoelectric Point (pI)

47.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WIT1_2_N	PF26581	35 - 174	1.6e-56	WIT1/2, N-terminal helical bundle
Tropomyosin	PF00261	305 - 524	6.2e-06	Tropomyosin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003026)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68910 AT1G68910 AT1G68910
fragaria_vesca	FvH4_4g28890 FvH4_4g28890 FvH4_4g28890 FvH4_4g28890 FvH4_4g28890 FvH4_4g28890 FvH4_4g28890 FvH4_4g28890 FvH4_4g28890 FvH4_4g28890
malus_domestica	MD13G1054600.v1.1 MD16G1053400.v1.1
prunus_persica	Prupe.1G297300_v2.0.a1 Prupe.1G297300_v2.0.a1 Prupe.1G297300_v2.0.a1 Prupe.1G297300_v2.0.a1 Prupe.1G297300_v2.0.a1 Prupe.1G297300_v2.0.a1 Prupe.1G297300_v2.0.a1 Prupe.1G297300_v2.0.a1 Prupe.1G297300_v2.0.a1
pyrus_communis	pycom13g04840 pycom16g04660
rosa_chinensis	RchiOBHm_Chr4g0437011
rosa_laevigata	RLG00000006478
rosa_multiflora	Rmu_sc0008325.1_g000019
rosa_roxburghii	Rroxscaffold_5G00378090
rosa_rugosa	Rorug04G0297700 Rorug04G0297800 Rorug04G0297900
rosa_samantha	Rh4AG352100 Rh4BG361200 Rh4CG375600 Rh4DG355400
rosa_wichuraiana	Rw4G030830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	73
AccI	GTMKAC	2 cut(s)	388, 850
AccII	CGCG	1 cut(s)	367
AciI	CCGC	3 cut(s)	1202, 1439, 1589
AcoI	YGGCCR	2 cut(s)	228, 252
AcsI	RAATTY	6 cut(s)	568, 601, 908, 1243, 1538, 1697
AcuI	CTGAAG	5 cut(s)	459, 1094, 1214, 2015, 2191
AdeI	CACNNNGTG	1 cut(s)	2279
AfaI	GTAC	7 cut(s)	183, 194, 315, 1459, 1792, 1974, 2000
AfiI	CCNNNNNNNGG	1 cut(s)	70
AjnI	CCWGG	6 cut(s)	822, 1860, 1888, 2046, 2140, 2180
AjuI	GAANNNNNNNTTGG	6 cut(s)	1098, 1130, 1266, 1298, 1792, 1824
Alw26I	GTCTC	3 cut(s)	619, 1295, 1877
Ama87I	CYCGRG	3 cut(s)	218, 968, 1292
AoxI	GGCC	4 cut(s)	228, 252, 1176, 1977
ApeKI	GCWGC	9 cut(s)	767, 809, 856, 1013, 1300, 1363, 1382, 2135, 2165
ApoI	RAATTY	6 cut(s)	568, 601, 908, 1243, 1538, 1697
Asp700I	GAANNNNTTC	1 cut(s)	2150
AspLEI	GCGC	3 cut(s)	367, 1659, 2140
AsuC2I	CCSGG	1 cut(s)	2215
AsuHPI	GGTGA	3 cut(s)	1042, 1222, 2116
AvaI	CYCGRG	3 cut(s)	218, 968, 1292
BalI	TGGCCA	1 cut(s)	230
BanI	GGYRCC	1 cut(s)	73
BarI	GAAGNNNNNNTAC	2 cut(s)	1302, 1334
BbsI	GAAGAC	2 cut(s)	12, 1761
BbvCI	CCTCAGC	2 cut(s)	813, 1304
BbvI	GCAGC	9 cut(s)	779, 796, 868, 1000, 1312, 1369, 1375, 2147, 2152
BccI	CCATC	2 cut(s)	85, 2117
BciT130I	CCWGG	6 cut(s)	824, 1862, 1890, 2048, 2142, 2182
BcnI	CCSGG	1 cut(s)	2215
BcoDI	GTCTC	3 cut(s)	619, 1295, 1877
BfaI	CTAG	4 cut(s)	294, 515, 615, 924
BfmI	CTRYAG	3 cut(s)	854, 1644, 2166
BfoI	RGCGCY	1 cut(s)	2141
BglII	AGATCT	1 cut(s)	1414
BisI	GCNGC	9 cut(s)	768, 810, 857, 1014, 1301, 1364, 1383, 2136, 2166
BlpI	GCTNAGC	1 cut(s)	204
BlsI	GCNGC	9 cut(s)	769, 811, 858, 1015, 1302, 1365, 1384, 2137, 2167
BmcAI	AGTACT	1 cut(s)	1459
Bme1390I	CCNGG	7 cut(s)	824, 1862, 1890, 2048, 2142, 2182, 2215
BmeT110I	CYCGRG	3 cut(s)	218, 968, 1292
BmiI	GGNNCC	2 cut(s)	75, 2052
BmrFI	CCNGG	7 cut(s)	824, 1862, 1890, 2048, 2142, 2182, 2215
BpiI	GAAGAC	2 cut(s)	12, 1761
BpmI	CTGGAG	3 cut(s)	665, 806, 1052
Bpu10I	CCTNAGC	3 cut(s)	813, 1304, 1988
Bpu1102I	GCTNAGC	1 cut(s)	204
BpuEI	CTTGAG	3 cut(s)	68, 695, 1061
BpuMI	CCSGG	1 cut(s)	2215
BsaJI	CCNNGG	6 cut(s)	474, 552, 823, 1824, 2141, 2180
BsaWI	WCCGGW	1 cut(s)	2188
Bsc4I	CCNNNNNNNGG	1 cut(s)	70
Bse1I	ACTGG	6 cut(s)	1014, 1270, 1644, 1917, 1942, 2255
Bse3DI	GCAATG	6 cut(s)	231, 546, 600, 1475, 1638, 2227
BseBI	CCWGG	6 cut(s)	824, 1862, 1890, 2048, 2142, 2182
BseDI	CCNNGG	6 cut(s)	474, 552, 823, 1824, 2141, 2180
BseGI	GGATG	8 cut(s)	60, 615, 645, 1340, 1659, 1820, 1873, 2176
BseLI	CCNNNNNNNGG	1 cut(s)	70
BseMI	GCAATG	6 cut(s)	231, 546, 600, 1475, 1638, 2227
BseMII	CTCAG	8 cut(s)	150, 218, 300, 804, 1295, 1452, 1731, 1979
BseNI	ACTGG	6 cut(s)	1014, 1270, 1644, 1917, 1942, 2255
BseRI	GAGGAG	1 cut(s)	1478
BseXI	GCAGC	9 cut(s)	779, 796, 868, 1000, 1312, 1369, 1375, 2147, 2152
BsgI	GTGCAG	1 cut(s)	1206
Bsh1236I	CGCG	1 cut(s)	367
BshFI	GGCC	4 cut(s)	230, 254, 1178, 1979
BshNI	GGYRCC	1 cut(s)	73
BsiHKCI	CYCGRG	3 cut(s)	218, 968, 1292
BsiSI	CCGG	2 cut(s)	2189, 2215
BslFI	GGGAC	1 cut(s)	1917
BslI	CCNNNNNNNGG	1 cut(s)	70
BsmAI	GTCTC	3 cut(s)	619, 1295, 1877
BsmFI	GGGAC	1 cut(s)	1917
BsmI	GAATGC	2 cut(s)	1084, 2120
BsnI	GGCC	4 cut(s)	230, 254, 1178, 1979
BsoBI	CYCGRG	3 cut(s)	218, 968, 1292
Bsp143I	GATC	2 cut(s)	214, 1414
Bsp1720I	GCTNAGC	1 cut(s)	204
Bsp19I	CCATGG	1 cut(s)	552
BspACI	CCGC	3 cut(s)	1202, 1439, 1589
BspANI	GGCC	4 cut(s)	230, 254, 1178, 1979
BspCNI	CTCAG	8 cut(s)	151, 217, 301, 805, 1296, 1453, 1732, 1980
BspFNI	CGCG	1 cut(s)	367
BspLI	GGNNCC	2 cut(s)	75, 2052
BspMAI	CTGCAG	2 cut(s)	858, 2170
BspQI	GCTCTTC	1 cut(s)	1230
BspT107I	GGYRCC	1 cut(s)	73
BsrDI	GCAATG	6 cut(s)	231, 546, 600, 1475, 1638, 2227
BsrI	ACTGG	6 cut(s)	1014, 1270, 1644, 1917, 1942, 2255
BssECI	CCNNGG	6 cut(s)	474, 552, 823, 1824, 2141, 2180
BssMI	GATC	2 cut(s)	214, 1414
BssT1I	CCWWGG	1 cut(s)	552
Bst2UI	CCWGG	6 cut(s)	824, 1862, 1890, 2048, 2142, 2182
Bst4CI	ACNGT	6 cut(s)	405, 511, 1519, 1875, 1963, 2277
Bst6I	CTCTTC	4 cut(s)	735, 927, 1101, 1230
BstAPI	GCANNNNNTGC	3 cut(s)	806, 1580, 2162
BstC8I	GCNNGC	2 cut(s)	95, 1332
BstDEI	CTNAG	8 cut(s)	159, 204, 309, 813, 1304, 1461, 1740, 1988
BstDSI	CCRYGG	2 cut(s)	552, 1824
BstF5I	GGATG	8 cut(s)	60, 615, 645, 1340, 1659, 1820, 1873, 2176
BstFNI	CGCG	1 cut(s)	367
BstH2I	RGCGCY	1 cut(s)	2141
BstHHI	GCGC	3 cut(s)	367, 1659, 2140
BstKTI	GATC	2 cut(s)	217, 1417
BstMAI	GTCTC	3 cut(s)	619, 1295, 1877
BstMBI	GATC	2 cut(s)	214, 1414
BstMWI	GCNNNNNNNGC	6 cut(s)	460, 806, 1184, 1580, 2111, 2162
BstNI	CCWGG	6 cut(s)	824, 1862, 1890, 2048, 2142, 2182
BstNSI	RCATGY	2 cut(s)	1334, 2222
BstSCI	CCNGG	7 cut(s)	822, 1860, 1888, 2046, 2140, 2180, 2213
BstSFI	CTRYAG	3 cut(s)	854, 1644, 2166
BstUI	CGCG	1 cut(s)	367
BstV1I	GCAGC	9 cut(s)	779, 796, 868, 1000, 1312, 1369, 1375, 2147, 2152
BstV2I	GAAGAC	2 cut(s)	12, 1761
BstX2I	RGATCY	1 cut(s)	1414
BstYI	RGATCY	1 cut(s)	1414
BsuRI	GGCC	4 cut(s)	230, 254, 1178, 1979
BtgI	CCRYGG	2 cut(s)	552, 1824
BtgZI	GCGATG	1 cut(s)	2095
BtsCI	GGATG	8 cut(s)	60, 615, 645, 1340, 1659, 1820, 1873, 2176
BtsI	GCAGTG	1 cut(s)	2160
BtsIMutI	CAGTG	4 cut(s)	1935, 1959, 2160, 2282
Cac8I	GCNNGC	2 cut(s)	95, 1332
CfoI	GCGC	3 cut(s)	367, 1659, 2140
CseI	GACGC	3 cut(s)	356, 1042, 1128
Csp6I	GTAC	7 cut(s)	182, 193, 314, 1458, 1791, 1973, 1999
CspCI	CAANNNNNGTGG	1 cut(s)	34
CviQI	GTAC	7 cut(s)	182, 193, 314, 1458, 1791, 1973, 1999
DdeI	CTNAG	8 cut(s)	159, 204, 309, 813, 1304, 1461, 1740, 1988
DpnI	GATC	2 cut(s)	216, 1416
DpnII	GATC	2 cut(s)	214, 1414
DraIII	CACNNNGTG	1 cut(s)	2279
EaeI	YGGCCR	2 cut(s)	228, 252
Eam1104I	CTCTTC	4 cut(s)	735, 927, 1101, 1230
EarI	CTCTTC	4 cut(s)	735, 927, 1101, 1230
Eco130I	CCWWGG	1 cut(s)	552
Eco32I	GATATC	1 cut(s)	25
Eco57I	CTGAAG	5 cut(s)	459, 1094, 1214, 2015, 2191
Eco88I	CYCGRG	3 cut(s)	218, 968, 1292
EcoRI	GAATTC	1 cut(s)	1538
EcoRII	CCWGG	6 cut(s)	822, 1860, 1888, 2046, 2140, 2180
EcoRV	GATATC	1 cut(s)	25
EcoT14I	CCWWGG	1 cut(s)	552
EcoT22I	ATGCAT	2 cut(s)	732, 2122
ErhI	CCWWGG	1 cut(s)	552
FalI	AAGNNNNNCTT	6 cut(s)	122, 154, 699, 731, 1098, 1130
FaqI	GGGAC	1 cut(s)	1917
FauNDI	CATATG	2 cut(s)	735, 2116
FblI	GTMKAC	2 cut(s)	388, 850
Fnu4HI	GCNGC	9 cut(s)	768, 810, 857, 1014, 1301, 1364, 1383, 2136, 2166
FokI	GGATG	8 cut(s)	47, 622, 652, 1327, 1646, 1807, 1880, 2163
Fsp4HI	GCNGC	9 cut(s)	768, 810, 857, 1014, 1301, 1364, 1383, 2136, 2166
FspBI	CTAG	4 cut(s)	294, 515, 615, 924
GlaI	GCGC	3 cut(s)	366, 1658, 2139
GluI	GCNGC	9 cut(s)	768, 810, 857, 1014, 1301, 1364, 1383, 2136, 2166
GsuI	CTGGAG	3 cut(s)	665, 806, 1052
HaeII	RGCGCY	1 cut(s)	2141
HaeIII	GGCC	4 cut(s)	230, 254, 1178, 1979
HapII	CCGG	2 cut(s)	2189, 2215
HgaI	GACGC	3 cut(s)	356, 1042, 1128
HhaI	GCGC	3 cut(s)	367, 1659, 2140
Hin6I	GCGC	3 cut(s)	365, 1657, 2138
HinP1I	GCGC	3 cut(s)	365, 1657, 2138
HincII	GTYRAC	1 cut(s)	851
HindII	GTYRAC	1 cut(s)	851
HpaII	CCGG	2 cut(s)	2189, 2215
HphI	GGTGA	3 cut(s)	1042, 1222, 2116
Hpy166II	GTNNAC	4 cut(s)	136, 389, 851, 1791
Hpy8I	GTNNAC	4 cut(s)	136, 389, 851, 1791
HpyAV	CCTTC	5 cut(s)	780, 1189, 1420, 2092, 2161
HpyCH4III	ACNGT	6 cut(s)	405, 511, 1519, 1875, 1963, 2277
HpyCH4IV	ACGT	1 cut(s)	2001
HpyF10VI	GCNNNNNNNGC	6 cut(s)	460, 806, 1184, 1580, 2111, 2162
HpyF3I	CTNAG	8 cut(s)	159, 204, 309, 813, 1304, 1461, 1740, 1988
HpySE526I	ACGT	1 cut(s)	2001
HspAI	GCGC	3 cut(s)	365, 1657, 2138
Kzo9I	GATC	2 cut(s)	214, 1414
LguI	GCTCTTC	1 cut(s)	1230
LmnI	GCTCC	6 cut(s)	664, 1041, 1071, 1465, 1654, 2216
Lsp1109I	GCAGC	9 cut(s)	779, 796, 868, 1000, 1312, 1369, 1375, 2147, 2152
MaeI	CTAG	4 cut(s)	294, 515, 615, 924
MaeII	ACGT	1 cut(s)	2001
MaeIII	GTNAC	5 cut(s)	1030, 1059, 1207, 1834, 2271
MalI	GATC	2 cut(s)	216, 1416
MboI	GATC	2 cut(s)	214, 1414
MflI	RGATCY	1 cut(s)	1414
MlsI	TGGCCA	1 cut(s)	230
MluNI	TGGCCA	1 cut(s)	230
MlyI	GAGTC	1 cut(s)	1067
MmeI	TCCRAC	1 cut(s)	1264
Mox20I	TGGCCA	1 cut(s)	230
Mph1103I	ATGCAT	2 cut(s)	732, 2122
MroXI	GAANNNNTTC	1 cut(s)	2150
MscI	TGGCCA	1 cut(s)	230
MslI	CAYNNNNRTG	4 cut(s)	63, 551, 2232, 2238
Msp20I	TGGCCA	1 cut(s)	230
MspA1I	CMGCKG	3 cut(s)	1051, 1072, 1303
MspI	CCGG	2 cut(s)	2189, 2215
MspR9I	CCNGG	7 cut(s)	824, 1862, 1890, 2048, 2142, 2182, 2215
Mva1269I	GAATGC	2 cut(s)	1084, 2120
MvaI	CCWGG	6 cut(s)	824, 1862, 1890, 2048, 2142, 2182
MvnI	CGCG	1 cut(s)	367
MwoI	GCNNNNNNNGC	6 cut(s)	460, 806, 1184, 1580, 2111, 2162
NciI	CCSGG	1 cut(s)	2215
NcoI	CCATGG	1 cut(s)	552
NdeI	CATATG	2 cut(s)	735, 2116
NdeII	GATC	2 cut(s)	214, 1414
NlaIV	GGNNCC	2 cut(s)	75, 2052
NmeAIII	GCCGAG	2 cut(s)	439, 1204
NmuCI	GTSAC	4 cut(s)	1030, 1059, 1834, 2271
NsiI	ATGCAT	2 cut(s)	732, 2122
NspI	RCATGY	2 cut(s)	1334, 2222
PaeI	GCATGC	1 cut(s)	1334
PaeR7I	CTCGAG	3 cut(s)	218, 968, 1292
PciSI	GCTCTTC	1 cut(s)	1230
PcsI	WCGNNNNNNNCGW	2 cut(s)	90, 1097
PctI	GAATGC	2 cut(s)	1084, 2120
PdmI	GAANNNNTTC	1 cut(s)	2150
PfeI	GAWTC	9 cut(s)	86, 305, 434, 443, 692, 1097, 1608, 1841, 1946
PflFI	GACNNNGTC	1 cut(s)	1961
PkrI	GCNGC	9 cut(s)	769, 811, 858, 1015, 1302, 1365, 1384, 2137, 2167
PleI	GAGTC	1 cut(s)	1066
PpsI	GAGTC	1 cut(s)	1066
Psp6I	CCWGG	6 cut(s)	822, 1860, 1888, 2046, 2140, 2180
PspGI	CCWGG	6 cut(s)	822, 1860, 1888, 2046, 2140, 2180
PspN4I	GGNNCC	2 cut(s)	75, 2052
PstI	CTGCAG	2 cut(s)	858, 2170
PsuI	RGATCY	1 cut(s)	1414
PsyI	GACNNNGTC	1 cut(s)	1961
PvuII	CAGCTG	3 cut(s)	1051, 1072, 1303
RsaI	GTAC	7 cut(s)	183, 194, 315, 1459, 1792, 1974, 2000
RsaNI	GTAC	7 cut(s)	182, 193, 314, 1458, 1791, 1973, 1999
RseI	CAYNNNNRTG	4 cut(s)	63, 551, 2232, 2238
SalI	GTCGAC	1 cut(s)	849
SapI	GCTCTTC	1 cut(s)	1230
SatI	GCNGC	9 cut(s)	768, 810, 857, 1014, 1301, 1364, 1383, 2136, 2166
Sau3AI	GATC	2 cut(s)	214, 1414
ScaI	AGTACT	1 cut(s)	1459
SchI	GAGTC	1 cut(s)	1067
ScrFI	CCNGG	7 cut(s)	824, 1862, 1890, 2048, 2142, 2182, 2215
SfcI	CTRYAG	3 cut(s)	854, 1644, 2166
Sfr274I	CTCGAG	3 cut(s)	218, 968, 1292
SlaI	CTCGAG	3 cut(s)	218, 968, 1292
SmiMI	CAYNNNNRTG	4 cut(s)	63, 551, 2232, 2238
SmlI	CTYRAG	6 cut(s)	47, 218, 674, 968, 1040, 1292
SmoI	CTYRAG	6 cut(s)	47, 218, 674, 968, 1040, 1292
SphI	GCATGC	1 cut(s)	1334
SsiI	CCGC	3 cut(s)	1202, 1439, 1589
SspMI	CTAG	4 cut(s)	294, 515, 615, 924
StyD4I	CCNGG	7 cut(s)	822, 1860, 1888, 2046, 2140, 2180, 2213
StyI	CCWWGG	1 cut(s)	552
TaaI	ACNGT	6 cut(s)	405, 511, 1519, 1875, 1963, 2277
TaiI	ACGT	1 cut(s)	2004
TaqI	TCGA	9 cut(s)	219, 581, 659, 850, 969, 1100, 1293, 1424, 1965
TatI	WGTACW	2 cut(s)	1457, 1790
TfiI	GAWTC	9 cut(s)	86, 305, 434, 443, 692, 1097, 1608, 1841, 1946
TscAI	CASTG	4 cut(s)	1942, 1966, 2167, 2282
TseFI	GTSAC	4 cut(s)	1030, 1059, 1834, 2271
TseI	GCWGC	9 cut(s)	767, 809, 856, 1013, 1300, 1363, 1382, 2135, 2165
Tsp45I	GTSAC	4 cut(s)	1030, 1059, 1834, 2271
TspDTI	ATGAA	9 cut(s)	81, 131, 431, 543, 720, 854, 995, 1110, 1884
TspGWI	ACGGA	1 cut(s)	1813
TspRI	CASTG	4 cut(s)	1942, 1966, 2167, 2282
Tth111I	GACNNNGTC	1 cut(s)	1961
XapI	RAATTY	6 cut(s)	568, 601, 908, 1243, 1538, 1697
XceI	RCATGY	2 cut(s)	1334, 2222
XhoI	CTCGAG	3 cut(s)	218, 968, 1292
XmiI	GTMKAC	2 cut(s)	388, 850
XmnI	GAANNNNTTC	1 cut(s)	2150
XspI	CTAG	4 cut(s)	294, 515, 615, 924
ZrmI	AGTACT	1 cut(s)	1459
Zsp2I	ATGCAT	2 cut(s)	732, 2122

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.