pycom16g07220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0018432
Physical Location & Seq
Forward (+)
4702163 .. 4702711
549 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ATGAAACACAAGGGTAGTAGAACCACTAGTCGCAAGAACAAATCTCTGTGTGAAAAGTCAATGATTTTGGTGGCAAACGTCATTAAACTTTCATCTCTTTCTCTTGGCAATATCCATCTTCCAACAACCATGACCAGGCCTCCGCCAAATCCCGCAGGAGCGCCTGCTAAAGTTACCAAAGTAGACGTCCCTGATCCTCAAAATCCAAGAAGCCCGCCGAAAAGGTTGCAGGAATCAGAAAATGAATCAAAGCCGTTCTCCTTCGTGATGCAGTTACCAAGCACAGATCAGAATACTGGTACTACTATTGCTGCTACTACAACTACAAGTACTCAGCCATGGGACGCGAGTAAGAATAGTAAGAGCGAAGCAGGCAGCGATGTTGATCGGAAAGCTGAAGCTTATATTAAAAGGATGCGTGACAAGATTCAGAACCAAAGCGATCAACCAGCAGTGAACCCTACTCCTAGTACTACTACGGCTGGAATAGTATCATCCAAACGTCCTTCACATGCACGGCCTGCCCCAGCTCCTAAAATATCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

19.64

Weight (kDa)

10.22

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018432)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35350
pyrus_communis pycom13g07250 pycom16g07220
rosa_laevigata RLG00000005782
rosa_roxburghii Rroxscaffold_5G00385460
rosa_rugosa Rorug04G0356800
rosa_samantha Rh4AG418400 Rh4BG430100 Rh4CG444900 Rh4DG426100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
AccI GTMKAC 1 cut(s) 183
AccII CGCG 1 cut(s) 347
AciI CCGC 3 cut(s) 143, 153, 215
AclWI GGATC 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 417
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 3 cut(s) 301, 331, 472
AfiI CCNNNNNNNGG 1 cut(s) 135
AhlI ACTAGT 1 cut(s) 26
AjnI CCWGG 1 cut(s) 134
AluBI AGCT 3 cut(s) 395, 401, 530
AluI AGCT 3 cut(s) 395, 401, 530
AlwI GGATC 1 cut(s) 188
AoxI GGCC 2 cut(s) 137, 518
ApeKI GCWGC 2 cut(s) 311, 375
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AspLEI GCGC 1 cut(s) 163
BbvI GCAGC 2 cut(s) 298, 387
BccI CCATC 1 cut(s) 123
BceAI ACGGC 3 cut(s) 238, 495, 533
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciT130I CCWGG 1 cut(s) 136
BcuI ACTAGT 1 cut(s) 26
BfaI CTAG 2 cut(s) 27, 468
BfoI RGCGCY 1 cut(s) 164
BisI GCNGC 2 cut(s) 312, 376
BlsI GCNGC 2 cut(s) 313, 377
BmcAI AGTACT 2 cut(s) 331, 472
Bme1390I CCNGG 1 cut(s) 136
BmrFI CCNGG 1 cut(s) 136
BmsI GCATC 2 cut(s) 258, 405
BsaBI GATNNNNATC 1 cut(s) 384
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 1 cut(s) 135
Bse1I ACTGG 1 cut(s) 301
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 1 cut(s) 136
BseDI CCNNGG 1 cut(s) 338
BseGI GGATG 2 cut(s) 420, 494
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 1 cut(s) 135
BseMII CTCAG 1 cut(s) 347
BseNI ACTGG 1 cut(s) 301
BseXI GCAGC 2 cut(s) 298, 387
BseYI CCCAGC 1 cut(s) 526
Bsh1236I CGCG 1 cut(s) 347
BshFI GGCC 2 cut(s) 139, 520
BslFI GGGAC 2 cut(s) 173, 356
BslI CCNNNNNNNGG 1 cut(s) 135
BsmFI GGGAC 2 cut(s) 173, 356
BsnI GGCC 2 cut(s) 139, 520
Bsp143I GATC 4 cut(s) 193, 286, 385, 442
Bsp19I CCATGG 1 cut(s) 338
BspACI CCGC 3 cut(s) 143, 153, 215
BspANI GGCC 2 cut(s) 139, 520
BspCNI CTCAG 1 cut(s) 346
BspFNI CGCG 1 cut(s) 347
BspPI GGATC 1 cut(s) 188
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 4 cut(s) 193, 286, 385, 442
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 1 cut(s) 338
Bst2UI CCWGG 1 cut(s) 136
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 1 cut(s) 521
BstC8I GCNNGC 4 cut(s) 165, 215, 373, 522
BstDEI CTNAG 1 cut(s) 333
BstDSI CCRYGG 1 cut(s) 338
BstF5I GGATG 2 cut(s) 420, 494
BstFNI CGCG 1 cut(s) 347
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 4 cut(s) 196, 289, 388, 445
BstMBI GATC 4 cut(s) 193, 286, 385, 442
BstMWI GCNNNNNNNGC 2 cut(s) 372, 521
BstNI CCWGG 1 cut(s) 136
BstNSI RCATGY 1 cut(s) 515
BstSCI CCNGG 1 cut(s) 134
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 2 cut(s) 298, 387
BsuRI GGCC 2 cut(s) 139, 520
BtgI CCRYGG 1 cut(s) 338
BtgZI GCGATG 1 cut(s) 393
BtsCI GGATG 2 cut(s) 420, 494
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 1 cut(s) 459
Cac8I GCNNGC 4 cut(s) 165, 215, 373, 522
CfoI GCGC 1 cut(s) 163
CseI GACGC 1 cut(s) 353
Csp6I GTAC 3 cut(s) 300, 330, 471
CviAII CATG 3 cut(s) 130, 339, 512
CviJI RGCY 9 cut(s) 139, 213, 253, 337, 395, 401, 482, 520, 530
CviKI_1 RGCY 9 cut(s) 139, 213, 253, 337, 395, 401, 482, 520, 530
CviQI GTAC 3 cut(s) 300, 330, 471
DdeI CTNAG 1 cut(s) 333
DpnI GATC 4 cut(s) 195, 288, 387, 444
DpnII GATC 4 cut(s) 193, 286, 385, 442
EciI GGCGGA 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 338
Eco147I AGGCCT 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 417
EcoRII CCWGG 1 cut(s) 134
EcoT14I CCWWGG 1 cut(s) 338
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 3 cut(s) 133, 342, 515
FaiI YATR 4 cut(s) 131, 340, 405, 513
FaqI GGGAC 2 cut(s) 173, 356
FatI CATG 3 cut(s) 129, 338, 511
FauI CCCGC 2 cut(s) 160, 222
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 2 cut(s) 312, 376
FokI GGATG 2 cut(s) 427, 481
Fsp4HI GCNGC 2 cut(s) 312, 376
FspBI CTAG 2 cut(s) 27, 468
GlaI GCGC 1 cut(s) 162
GluI GCNGC 2 cut(s) 312, 376
GsaI CCCAGC 1 cut(s) 530
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 2 cut(s) 139, 520
HgaI GACGC 1 cut(s) 353
HhaI GCGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 3 cut(s) 133, 342, 515
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 3 cut(s) 233, 245, 427
Hpy166II GTNNAC 2 cut(s) 184, 457
Hpy188I TCNGA 4 cut(s) 238, 291, 390, 432
Hpy188III TCNNGA 1 cut(s) 265
Hpy8I GTNNAC 2 cut(s) 184, 457
HpyAV CCTTC 2 cut(s) 271, 516
HpyCH4IV ACGT 3 cut(s) 78, 186, 502
HpyCH4V TGCA 3 cut(s) 229, 271, 515
HpyF10VI GCNNNNNNNGC 2 cut(s) 372, 521
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 3 cut(s) 78, 186, 502
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 3 cut(s) 133, 342, 515
HspAI GCGC 1 cut(s) 161
Kzo9I GATC 4 cut(s) 193, 286, 385, 442
LmnI GCTCC 2 cut(s) 158, 535
Lsp1109I GCAGC 2 cut(s) 298, 387
LweI GCATC 2 cut(s) 258, 405
MaeI CTAG 2 cut(s) 27, 468
MaeII ACGT 3 cut(s) 78, 186, 502
MaeIII GTNAC 3 cut(s) 172, 273, 419
MalI GATC 4 cut(s) 195, 288, 387, 444
MboI GATC 4 cut(s) 193, 286, 385, 442
MboII GAAGA 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 146
MnlI CCTC 2 cut(s) 150, 207
MseI TTAA 2 cut(s) 84, 408
MspR9I CCNGG 1 cut(s) 136
MvaI CCWGG 1 cut(s) 136
MvnI CGCG 1 cut(s) 347
MwoI GCNNNNNNNGC 2 cut(s) 372, 521
NcoI CCATGG 1 cut(s) 338
NdeII GATC 4 cut(s) 193, 286, 385, 442
NlaIII CATG 3 cut(s) 133, 342, 515
NmuCI GTSAC 1 cut(s) 419
NspI RCATGY 1 cut(s) 515
PceI AGGCCT 1 cut(s) 139
PfeI GAWTC 3 cut(s) 233, 245, 427
PkrI GCNGC 2 cut(s) 313, 377
Psp6I CCWGG 1 cut(s) 134
PspFI CCCAGC 1 cut(s) 526
PspGI CCWGG 1 cut(s) 134
RsaI GTAC 3 cut(s) 301, 331, 472
RsaNI GTAC 3 cut(s) 300, 330, 471
SaqAI TTAA 2 cut(s) 84, 408
SatI GCNGC 2 cut(s) 312, 376
Sau3AI GATC 4 cut(s) 193, 286, 385, 442
ScaI AGTACT 2 cut(s) 331, 472
ScrFI CCNGG 1 cut(s) 136
SetI ASST 7 cut(s) 81, 189, 227, 397, 403, 505, 532
SfaNI GCATC 2 cut(s) 258, 405
SpeI ACTAGT 1 cut(s) 26
SseBI AGGCCT 1 cut(s) 139
SsiI CCGC 3 cut(s) 143, 153, 215
SspMI CTAG 2 cut(s) 27, 468
StuI AGGCCT 1 cut(s) 139
StyD4I CCNGG 1 cut(s) 134
StyI CCWWGG 1 cut(s) 338
TaiI ACGT 3 cut(s) 81, 189, 505
TatI WGTACW 2 cut(s) 329, 470
TfiI GAWTC 3 cut(s) 233, 245, 427
Tru1I TTAA 2 cut(s) 84, 408
Tru9I TTAA 2 cut(s) 84, 408
TscAI CASTG 1 cut(s) 459
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 2 cut(s) 311, 375
Tsp45I GTSAC 1 cut(s) 419
TspDTI ATGAA 3 cut(s) 17, 81, 258
TspRI CASTG 1 cut(s) 459
XceI RCATGY 1 cut(s) 515
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 2 cut(s) 27, 468
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 2 cut(s) 331, 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.