pycom16g08300

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005807
Physical Location & Seq
Reverse (-)
5500983 .. 5502909
1927 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1356 bp
ATGTCCAAATTTATCTTACTTTGGTTCTTTCCTCTGTTTTTGTTTCTAAAGAGTGCTCGTTCTGATCCGAGGGCCACTCAGGCAGCGATGATATGCACAAACGACACAGCTCCGATCCCAGAACGTCAAACCTTCATCGCCAACTTCTTAGCAGCCATGGACGCCGTCACCCCACAGATTGCCACCCGAAGATACGCCACCGCCGTCACCGGAACTGGAAACGCCACCGTTTTCGCTTTCGGCGAGTGCATGAGAGACCTCTCCAAGCAAGACTGCGACCTCTGCTTCGCCCAGTGCAAGACCAAACTTTTAGGGTGCCTCCCGTTTCAGAAAGCAACTCTCGGCGGCCGCCTTTTTTACGACGGTTGCTATCTCCGGTACGATGATTTCGATTTCTTCAACCAGAGCTTGAGTCCCCAGGACAGGACCGTTTGTGGGGACAGAGATTTTGGTCGGAATCGGACAGTTTTTAAGGATAATGCTCTGAGGTTGGTGAGGAATTTGAGCTCGACGTCGCCTAAGAATGATGGGTTTTCTGCTGGGTTTGCGGTGGGTGAAGGGAACGTTTCGGTTTACGGGTTGGCGCAGTGTTGGGAGTTTGTGAAGGGCGCTGGTTGCGAGGAGTGTTTGGCTGACGCCGTTAGGAGAATCGGGTCTTGCCCTCCGAAAGAAGAAGGGAGGGTTTTGAATGCCGGTTGTTACTTGAGGTATTCAACTCAGAAGTTCTATGATAACAATACAGTCACCGATGATGTAGGCCGAAATGGAGGACGCCGCGGCTTGGCGATAACTTTGGCTGTAACATCTGCTGCATTTGCTGTTGTGCTGACTATTTCTGCACTTGCTTTCTGTGCAAGGAAGAAAATACTGACGAGAAGGAAAGCTGCTTCAGAGAAAAAGCAATTCGGAGCTCTGTTGGCCCATGTGAATAAGTCCCAACTCAATTTTTCATACGAAGTTCTCGAAAAGGCCACCAATTACTTTCATGATTCCAACAAGCTTGGACAAGGAGGATCTGGCTCAGTGTATAAGGGAGTTTTGCCGGATGGCAAGGTTGTTGCCATAAAGAGGCTGTTCTTCAACACAAGGCAATGGGTGGATCGATTCTTCAATGAAGTCAATTTGATCAGCGGAATCCATCATAAAAATCTTGTAAAGCTGTTGGGATGCAGCATTACAGGCCCCGAAAGCCTTCTTGTTTATGAGTATGTCCCAAATCAAAGCCTTCATGATTATTTTAATGTGAAAAATAATGTGGAGCCACTGAGGTGGGATTTGAGGTATAAAATCATACTGGGAACAGCTGAGGGCTTGGCCTATCTTCACGAAGAATCGAAATTGATGGATCATTCATAG

Protein Analysis

452

Amino Acids

50.23

Weight (kDa)

9.03

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 32 - 129 7.2e-12 Salt stress response/antifungal
Stress-antifung PF01657 150 - 240 2.3e-15 Salt stress response/antifungal
Pkinase PF00069 328 - 444 3.1e-16 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 331 - 444 4.3e-16 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 515
AccB1I GGYRCC 1 cut(s) 315
AccII CGCG 1 cut(s) 777
AciI CCGC 7 cut(s) 201, 345, 349, 548, 775, 777, 1131
AclI AACGTT 1 cut(s) 564
AclWI GGATC 5 cut(s) 59, 109, 1021, 1107, 1353
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 2 cut(s) 8, 499
AcuI CTGAAG 1 cut(s) 873
AcyI GRCGYC 4 cut(s) 162, 512, 636, 772
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 4 cut(s) 423, 435, 781, 1068
AgsI TTSAA 5 cut(s) 400, 688, 714, 1081, 1111
AjnI CCWGG 1 cut(s) 417
AleI CACNNNNGTG 1 cut(s) 1267
AluBI AGCT 8 cut(s) 110, 408, 507, 884, 911, 1000, 1159, 1304
AluI AGCT 8 cut(s) 110, 408, 507, 884, 911, 1000, 1159, 1304
Alw21I GWGCWC 3 cut(s) 58, 509, 913
Alw26I GTCTC 1 cut(s) 249
AlwI GGATC 5 cut(s) 59, 109, 1021, 1107, 1353
AoxI GGCC 7 cut(s) 72, 346, 757, 918, 969, 1180, 1314
ApeKI GCWGC 5 cut(s) 83, 152, 809, 884, 1170
ApoI RAATTY 2 cut(s) 8, 499
ArsI GACNNNNNNTTYG 4 cut(s) 269, 301, 431, 463
Asp700I GAANNNNTTC 1 cut(s) 1191
AspLEI GCGC 2 cut(s) 586, 611
AspS9I GGNCC 4 cut(s) 72, 426, 919, 1181
AsuHPI GGTGA 5 cut(s) 160, 199, 505, 566, 736
AvaII GGWCC 1 cut(s) 426
BanI GGYRCC 1 cut(s) 315
BanII GRGCYC 2 cut(s) 509, 913
Bbv12I GWGCWC 3 cut(s) 58, 509, 913
BbvCI CCTCAGC 1 cut(s) 1305
BbvI GCAGC 5 cut(s) 95, 164, 796, 871, 1182
BccI CCATC 4 cut(s) 521, 1040, 1146, 1336
BceAI ACGGC 3 cut(s) 149, 188, 623
BciT130I CCWGG 1 cut(s) 419
BclI TGATCA 1 cut(s) 1125
BcoDI GTCTC 1 cut(s) 249
BfoI RGCGCY 1 cut(s) 612
BglI GCCNNNNNGGC 1 cut(s) 80
BisI GCNGC 9 cut(s) 84, 153, 346, 349, 775, 778, 810, 885, 1171
BlsI GCNGC 9 cut(s) 85, 154, 347, 350, 776, 779, 811, 886, 1172
Bme1390I CCNGG 1 cut(s) 419
Bme18I GGWCC 1 cut(s) 426
BmgT120I GGNCC 4 cut(s) 72, 426, 919, 1181
BmiI GGNNCC 3 cut(s) 317, 1183, 1260
BmrFI CCNGG 1 cut(s) 419
BmrI ACTGGG 2 cut(s) 286, 1304
BmsI GCATC 1 cut(s) 1157
BmuI ACTGGG 2 cut(s) 286, 1304
BplI GAGNNNNNCTC 2 cut(s) 61, 93
Bpu10I CCTNAGC 1 cut(s) 1305
BpuEI CTTGAG 2 cut(s) 430, 724
Bsa29I ATCGAT 1 cut(s) 1102
BsaHI GRCGYC 4 cut(s) 162, 512, 636, 772
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 4 cut(s) 68, 156, 417, 775
BsaWI WCCGGW 2 cut(s) 209, 375
Bsc4I CCNNNNNNNGG 4 cut(s) 423, 435, 781, 1068
Bse118I RCCGGY 1 cut(s) 692
Bse1I ACTGG 3 cut(s) 220, 292, 1299
Bse3DI GCAATG 1 cut(s) 1097
BseBI CCWGG 1 cut(s) 419
BseCI ATCGAT 1 cut(s) 1102
BseDI CCNNGG 4 cut(s) 68, 156, 417, 775
BseGI GGATG 2 cut(s) 1051, 1172
BseLI CCNNNNNNNGG 4 cut(s) 423, 435, 781, 1068
BseMI GCAATG 1 cut(s) 1097
BseMII CTCAG 6 cut(s) 92, 476, 731, 1035, 1256, 1296
BseNI ACTGG 3 cut(s) 220, 292, 1299
BseRI GAGGAG 1 cut(s) 635
BseX3I CGGCCG 1 cut(s) 346
BseXI GCAGC 5 cut(s) 95, 164, 796, 871, 1182
BseYI CCCAGC 1 cut(s) 539
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 1 cut(s) 777
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 7 cut(s) 74, 348, 759, 920, 971, 1182, 1316
BshNI GGYRCC 1 cut(s) 315
BshVI ATCGAT 1 cut(s) 1102
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 3 cut(s) 58, 509, 913
BsiSI CCGG 4 cut(s) 210, 376, 693, 1043
BslFI GGGAC 4 cut(s) 399, 452, 919, 1196
BslI CCNNNNNNNGG 4 cut(s) 423, 435, 781, 1068
BsmAI GTCTC 1 cut(s) 249
BsmFI GGGAC 4 cut(s) 399, 452, 919, 1196
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 7 cut(s) 74, 348, 759, 920, 971, 1182, 1316
Bso31I GGTCTC 1 cut(s) 249
Bsp1286I GDGCHC 3 cut(s) 58, 509, 913
Bsp143I GATC 6 cut(s) 64, 114, 1013, 1099, 1125, 1345
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 7 cut(s) 201, 345, 349, 548, 775, 777, 1131
BspANI GGCC 7 cut(s) 74, 348, 759, 920, 971, 1182, 1316
BspCNI CTCAG 6 cut(s) 91, 477, 730, 1034, 1257, 1297
BspDI ATCGAT 1 cut(s) 1102
BspFNI CGCG 1 cut(s) 777
BspHI TCATGA 2 cut(s) 985, 1228
BspLI GGNNCC 3 cut(s) 317, 1183, 1260
BspPI GGATC 5 cut(s) 59, 109, 1021, 1107, 1353
BspT107I GGYRCC 1 cut(s) 315
BspTNI GGTCTC 1 cut(s) 249
BsrDI GCAATG 1 cut(s) 1097
BsrFI RCCGGY 1 cut(s) 692
BsrI ACTGG 3 cut(s) 220, 292, 1299
BssAI RCCGGY 1 cut(s) 692
BssECI CCNNGG 4 cut(s) 68, 156, 417, 775
BssMI GATC 6 cut(s) 64, 114, 1013, 1099, 1125, 1345
BssNI GRCGYC 4 cut(s) 162, 512, 636, 772
BssT1I CCWWGG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 5 cut(s) 229, 365, 430, 466, 742
BstACI GRCGYC 4 cut(s) 162, 512, 636, 772
BstDEI CTNAG 8 cut(s) 78, 148, 485, 519, 717, 1021, 1265, 1305
BstDSI CCRYGG 2 cut(s) 156, 775
BstF5I GGATG 2 cut(s) 1051, 1172
BstFNI CGCG 1 cut(s) 777
BstH2I RGCGCY 1 cut(s) 612
BstHHI GCGC 2 cut(s) 586, 611
BstKTI GATC 6 cut(s) 67, 117, 1016, 1102, 1128, 1348
BstMAI GTCTC 1 cut(s) 249
BstMBI GATC 6 cut(s) 64, 114, 1013, 1099, 1125, 1345
BstMCI CGRYCG 1 cut(s) 349
BstNI CCWGG 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 417
BstUI CGCG 1 cut(s) 777
BstV1I GCAGC 5 cut(s) 95, 164, 796, 871, 1182
BstX2I RGATCY 1 cut(s) 1013
BstXI CCANNNNNNTGG 1 cut(s) 1269
BstYI RGATCY 1 cut(s) 1013
BstZI CGGCCG 1 cut(s) 346
Bsu15I ATCGAT 1 cut(s) 1102
BsuRI GGCC 7 cut(s) 74, 348, 759, 920, 971, 1182, 1316
BsuTUI ATCGAT 1 cut(s) 1102
BtgI CCRYGG 2 cut(s) 156, 775
BtgZI GCGATG 2 cut(s) 101, 121
BtsCI GGATG 2 cut(s) 1051, 1172
BtsI GCAGTG 1 cut(s) 593
BtsIMutI CAGTG 4 cut(s) 299, 593, 1029, 1262
CciI TCATGA 2 cut(s) 985, 1228
CciNI GCGGCCGC 1 cut(s) 346
CfoI GCGC 2 cut(s) 586, 611
Cfr10I RCCGGY 1 cut(s) 692
Cfr13I GGNCC 4 cut(s) 72, 426, 919, 1181
Cfr42I CCGCGG 1 cut(s) 778
ClaI ATCGAT 1 cut(s) 1102
CseI GACGC 3 cut(s) 170, 644, 780
Csp6I GTAC 1 cut(s) 379
CviAII CATG 5 cut(s) 157, 250, 923, 986, 1229
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 8 cut(s) 78, 148, 485, 519, 717, 1021, 1265, 1305
DpnI GATC 6 cut(s) 66, 116, 1015, 1101, 1127, 1347
DpnII GATC 6 cut(s) 64, 114, 1013, 1099, 1125, 1345
EaeI YGGCCR 1 cut(s) 346
EagI CGGCCG 1 cut(s) 346
Ecl136II GAGCTC 2 cut(s) 507, 911
EclXI CGGCCG 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 156
Eco24I GRGCYC 2 cut(s) 509, 913
Eco31I GGTCTC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 426
Eco52I CGGCCG 1 cut(s) 346
Eco53kI GAGCTC 2 cut(s) 507, 911
Eco57I CTGAAG 1 cut(s) 873
EcoICRI GAGCTC 2 cut(s) 507, 911
EcoO109I RGGNCCY 1 cut(s) 1181
EcoRII CCWGG 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 156
EcoT38I GRGCYC 2 cut(s) 509, 913
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 5 cut(s) 160, 253, 926, 989, 1232
FaqI GGGAC 4 cut(s) 399, 452, 919, 1196
FatI CATG 5 cut(s) 156, 249, 922, 985, 1228
FbaI TGATCA 1 cut(s) 1125
Fnu4HI GCNGC 9 cut(s) 84, 153, 346, 349, 775, 778, 810, 885, 1171
FokI GGATG 2 cut(s) 1058, 1179
FriOI GRGCYC 2 cut(s) 509, 913
Fsp4HI GCNGC 9 cut(s) 84, 153, 346, 349, 775, 778, 810, 885, 1171
GlaI GCGC 2 cut(s) 585, 610
GluI GCNGC 9 cut(s) 84, 153, 346, 349, 775, 778, 810, 885, 1171
GsaI CCCAGC 1 cut(s) 543
HaeII RGCGCY 1 cut(s) 612
HaeIII GGCC 7 cut(s) 74, 348, 759, 920, 971, 1182, 1316
HapII CCGG 4 cut(s) 210, 376, 693, 1043
HgaI GACGC 3 cut(s) 170, 644, 780
HhaI GCGC 2 cut(s) 586, 611
Hin1I GRCGYC 4 cut(s) 162, 512, 636, 772
Hin1II CATG 5 cut(s) 160, 253, 926, 989, 1232
Hin6I GCGC 2 cut(s) 584, 609
HinP1I GCGC 2 cut(s) 584, 609
HindIII AAGCTT 1 cut(s) 998
HinfI GANTC 7 cut(s) 412, 457, 648, 989, 1104, 1134, 1331
HpaII CCGG 4 cut(s) 210, 376, 693, 1043
HphI GGTGA 5 cut(s) 160, 199, 505, 566, 736
Hpy166II GTNNAC 1 cut(s) 574
Hpy188III TCNNGA 4 cut(s) 962, 986, 1229, 1325
Hpy8I GTNNAC 1 cut(s) 574
Hpy99I CGWCG 3 cut(s) 365, 514, 517
HpyAV CCTTC 7 cut(s) 142, 551, 598, 668, 870, 1202, 1235
HpyCH4III ACNGT 5 cut(s) 229, 365, 430, 466, 742
HpyCH4IV ACGT 3 cut(s) 124, 512, 564
HpyCH4V TGCA 7 cut(s) 96, 249, 297, 812, 839, 854, 1170
HpyF3I CTNAG 8 cut(s) 78, 148, 485, 519, 717, 1021, 1265, 1305
HpySE526I ACGT 3 cut(s) 124, 512, 564
Hsp92I GRCGYC 4 cut(s) 162, 512, 636, 772
Hsp92II CATG 5 cut(s) 160, 253, 926, 989, 1232
HspAI GCGC 2 cut(s) 584, 609
Ksp22I TGATCA 1 cut(s) 1125
KspI CCGCGG 1 cut(s) 778
Kzo9I GATC 6 cut(s) 64, 114, 1013, 1099, 1125, 1345
LmnI GCTCC 3 cut(s) 115, 908, 1258
Lsp1109I GCAGC 5 cut(s) 95, 164, 796, 871, 1182
LweI GCATC 1 cut(s) 1157
MaeII ACGT 3 cut(s) 124, 512, 564
MaeIII GTNAC 5 cut(s) 166, 205, 698, 742, 799
MalI GATC 6 cut(s) 66, 116, 1015, 1101, 1127, 1347
MboI GATC 6 cut(s) 64, 114, 1013, 1099, 1125, 1345
MboII GAAGA 8 cut(s) 201, 388, 683, 871, 1069, 1099, 1313, 1340
MflI RGATCY 1 cut(s) 1013
MhlI GDGCHC 3 cut(s) 58, 509, 913
MluCI AATT 7 cut(s) 8, 499, 902, 943, 976, 1120, 1337
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 2 cut(s) 434, 1017
MroXI GAANNNNTTC 1 cut(s) 1191
MseI TTAA 2 cut(s) 471, 1239
MslI CAYNNNNRTG 1 cut(s) 1267
MspA1I CMGCKG 3 cut(s) 777, 1131, 1304
MspI CCGG 4 cut(s) 210, 376, 693, 1043
MspR9I CCNGG 1 cut(s) 419
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 1 cut(s) 419
MvnI CGCG 1 cut(s) 777
NcoI CCATGG 1 cut(s) 156
NdeII GATC 6 cut(s) 64, 114, 1013, 1099, 1125, 1345
NlaIII CATG 5 cut(s) 160, 253, 926, 989, 1232
NlaIV GGNNCC 3 cut(s) 317, 1183, 1260
NmeAIII GCCGAG 1 cut(s) 321
NmuCI GTSAC 3 cut(s) 166, 205, 742
NotI GCGGCCGC 1 cut(s) 346
OliI CACNNNNGTG 1 cut(s) 1267
PagI TCATGA 2 cut(s) 985, 1228
PcsI WCGNNNNNNNCGW 4 cut(s) 201, 240, 387, 960
PctI GAATGC 1 cut(s) 694
PdmI GAANNNNTTC 1 cut(s) 1191
PfeI GAWTC 6 cut(s) 457, 648, 989, 1104, 1134, 1331
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 9 cut(s) 85, 154, 347, 350, 776, 779, 811, 886, 1172
PleI GAGTC 1 cut(s) 420
PpsI GAGTC 1 cut(s) 420
Psp124BI GAGCTC 2 cut(s) 509, 913
Psp1406I AACGTT 1 cut(s) 564
Psp6I CCWGG 1 cut(s) 417
PspFI CCCAGC 1 cut(s) 539
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 3 cut(s) 317, 1183, 1260
PspPI GGNCC 4 cut(s) 72, 426, 919, 1181
PsuI RGATCY 1 cut(s) 1013
PsyI GACNNNGTC 1 cut(s) 164
PvuII CAGCTG 1 cut(s) 1304
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
RseI CAYNNNNRTG 1 cut(s) 1267
SacI GAGCTC 2 cut(s) 509, 913
SacII CCGCGG 1 cut(s) 778
SaqAI TTAA 2 cut(s) 471, 1239
SatI GCNGC 9 cut(s) 84, 153, 346, 349, 775, 778, 810, 885, 1171
Sau3AI GATC 6 cut(s) 64, 114, 1013, 1099, 1125, 1345
Sau96I GGNCC 4 cut(s) 72, 426, 919, 1181
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 1 cut(s) 419
SduI GDGCHC 3 cut(s) 58, 509, 913
SfaNI GCATC 1 cut(s) 1157
Sfr303I CCGCGG 1 cut(s) 778
SgrBI CCGCGG 1 cut(s) 778
SinI GGWCC 1 cut(s) 426
SmiMI CAYNNNNRTG 1 cut(s) 1267
SmlI CTYRAG 2 cut(s) 409, 703
SmoI CTYRAG 2 cut(s) 409, 703
Sse9I AATT 7 cut(s) 8, 499, 902, 943, 976, 1120, 1337
SsiI CCGC 7 cut(s) 201, 345, 349, 548, 775, 777, 1131
SstI GAGCTC 2 cut(s) 509, 913
StyD4I CCNGG 1 cut(s) 417
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 5 cut(s) 229, 365, 430, 466, 742
TaiI ACGT 3 cut(s) 127, 515, 567
TaqI TCGA 5 cut(s) 390, 509, 963, 1102, 1334
TasI AATT 7 cut(s) 8, 499, 902, 943, 976, 1120, 1337
TauI GCSGC 4 cut(s) 348, 351, 777, 780
TfiI GAWTC 6 cut(s) 457, 648, 989, 1104, 1134, 1331
Tru1I TTAA 2 cut(s) 471, 1239
Tru9I TTAA 2 cut(s) 471, 1239
TscAI CASTG 4 cut(s) 299, 593, 1029, 1269
TseFI GTSAC 3 cut(s) 166, 205, 742
TseI GCWGC 5 cut(s) 83, 152, 809, 884, 1170
Tsp45I GTSAC 3 cut(s) 166, 205, 742
TspDTI ATGAA 6 cut(s) 124, 939, 974, 1128, 1217, 1341
TspRI CASTG 4 cut(s) 299, 593, 1029, 1269
Tth111I GACNNNGTC 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 426
XapI RAATTY 2 cut(s) 8, 499
XmnI GAANNNNTTC 1 cut(s) 1191
ZraI GACGTC 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.