Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0016831

Physical Location & Seq

Genomic Coordinates Reverse (-)

5718411 .. 5719646

Gene Structure 1236 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g08590.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1236 bp

ATGGAAGAAGCGTTTAGAAGAATGAACGGGTTCTCTCATGCATCCGAACCCGACTTGATCACTGACCCTCCAAGCAAGTCAACAACCGCCGCCACCAACAAGCGCGCGCTGAGAGACACGGCGGCAGCCGCTGCCTCGGGCGCAATGAGATACCGCGGCGTGCGACGCAGGCCTTGGGGTCGTTACGCGGCGGAGATCAGAGACCCGCAGTCCAAAGAACGACGCTGGCTTGGGACCTTCGACACGGCAGAGGAAGCCGCCTGCGCCTACGACTGCGCCGCTCGTGCCATGCGCGGTGTCAAGGCGCGGACCAACTTCGTCTACTCTTCTTCCCCGCCTACCGACGTCGTCGCTGACCATCCTTTCCCTCATTTCAGCTTCCACAAGCACTCTCACCCGTCTGCCAAAACCCGTAACGGCTACGGTGGCGCCAACCACTCTCCTAGTTGGAATTCCGTACCGTTCTCGCAGTATCCATCTCCTAATCAGAACGACGGCTACGGCGGCGCCCCCACTCCTGCTGCAACATCTTTCAACATGCCTTTCCTCCAGAATTTCATGAAATATTCCTCACCAAACCCATCTCCCGTTTACAATAACCAGTTTCCGTACAATATCAACGGCTCTTCTTCAGCATCTTTGGTCAACACCACCTCTTACGGTCAACGCCATGAAACCAACATCTCCGGAAGTACTAAGTTCCCTATTGGTTCATCTTCTTCCATGGCTACTGCTCCTACTTCTACTACTACAACTCTTCCCTTCATAGATATTACTTCACCTCCTCCCACAAAACCCACCTCCCACGACTATCAACACGACTACTCCTCAGACTTCTTCCCCACGGAGCCGGCGTATTCCGGGCTGTTAGAGGAGGTGATCCATCGCTTCTTCCCAAAACCCACCAAAAAATCATCAAACGACGATCCCATATCGACTCAAGACTACGGCCATCCTTCCAGTTCCATTCAAACGGCTTGCTTTGACGGGTCTTCAACGAGGACAGTCGGAGGGTACTCAAAAAATGAGCAGAGTCAGGTAAATGTTTGTTACAATTTCCACGAGCTGCTGCCGCTGCCTCAGCCGCAGCAGCAGCAAGGAAACAGTCTCAACGGACTAATGGATCGATCACCAACAGCAAGTGTCACAGCCACAGGCAGTACCGGTCTACAACGATCACGTGCCGTTCAACTTCCAGATGGGCGGCGGCGCAGATCACTCCATGGGAATGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009889 GO:0010033 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051726 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0080090 GO:0090708 GO:0099402 GO:0140110 GO:1903506 GO:1905392 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AP2

Protein Analysis

412

Amino Acids

44.91

Weight (kDa)

9.24

Isoelectric Point (pI)

65.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AP2	PF00847	50 - 99	2.5e-12	AP2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016831)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25630
malus_domestica	MD13G1101100.v1.1 MD16G1101400.v1.1
prunus_persica	Prupe.1G245500_v2.0.a1
pyrus_communis	pycom13g08820 pycom16g08590
rosa_chinensis	RchiOBHm_Chr4g0433071
rosa_laevigata	RLG00000006789
rosa_multiflora	Rmu_sc0001190.1_g000018
rosa_rugosa	Rorug04G0265000
rosa_samantha	Rh4BG327200 Rh4CG342600 Rh4DG322200
rosa_wichuraiana	Rw4G027730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	348
AccB1I	GGYRCC	2 cut(s)	428, 506
AccBSI	CCGCTC	1 cut(s)	281
AccI	GTMKAC	2 cut(s)	321, 1168
AccII	CGCG	6 cut(s)	105, 107, 156, 188, 294, 307
AccIII	TCCGGA	1 cut(s)	686
AclWI	GGATC	3 cut(s)	874, 920, 1131
AcoI	YGGCCR	1 cut(s)	949
AcsI	RAATTY	2 cut(s)	451, 553
AcuI	CTGAAG	1 cut(s)	615
AcvI	CACGTG	1 cut(s)	1181
AcyI	GRCGYC	3 cut(s)	345, 429, 507
AfaI	GTAC	5 cut(s)	459, 611, 694, 1016, 1162
AgeI	ACCGGT	1 cut(s)	1163
AgsI	TTSAA	4 cut(s)	535, 971, 996, 1190
AhdI	GACNNNNNGTC	1 cut(s)	208
AluBI	AGCT	2 cut(s)	378, 1066
AluI	AGCT	2 cut(s)	378, 1066
Alw26I	GTCTC	3 cut(s)	108, 195, 1112
AlwI	GGATC	3 cut(s)	874, 920, 1131
AlwNI	CAGNNNCTG	1 cut(s)	131
Ama87I	CYCGRG	1 cut(s)	136
Aor13HI	TCCGGA	1 cut(s)	686
AoxI	GGCC	2 cut(s)	170, 949
ApeKI	GCWGC	9 cut(s)	125, 131, 521, 1066, 1069, 1075, 1087, 1090, 1093
ApoI	RAATTY	2 cut(s)	451, 553
AsiGI	ACCGGT	1 cut(s)	1163
Asp700I	GAANNNNTTC	1 cut(s)	29
AspS9I	GGNCC	2 cut(s)	234, 309
AsuC2I	CCSGG	1 cut(s)	862
AsuHPI	GGTGA	5 cut(s)	386, 564, 771, 889, 1122
AvaI	CYCGRG	1 cut(s)	136
AvaII	GGWCC	2 cut(s)	234, 309
BanI	GGYRCC	2 cut(s)	428, 506
BauI	CACGAG	2 cut(s)	282, 1061
BbrPI	CACGTG	1 cut(s)	1181
BbsI	GAAGAC	1 cut(s)	984
BbvCI	CCTCAGC	1 cut(s)	1080
BbvI	GCAGC	9 cut(s)	118, 137, 508, 1053, 1056, 1062, 1099, 1102, 1105
BccI	CCATC	6 cut(s)	366, 484, 589, 891, 960, 1193
BceAI	ACGGC	9 cut(s)	135, 261, 433, 511, 517, 637, 964, 990, 1169
BciVI	GTATCC	1 cut(s)	483
BclI	TGATCA	1 cut(s)	57
BcnI	CCSGG	1 cut(s)	862
BcoDI	GTCTC	3 cut(s)	108, 195, 1112
BfaI	CTAG	1 cut(s)	444
BfoI	RGCGCY	2 cut(s)	432, 510
BfuI	GTATCC	1 cut(s)	483
BmcAI	AGTACT	1 cut(s)	694
Bme1390I	CCNGG	1 cut(s)	862
Bme18I	GGWCC	2 cut(s)	234, 309
BmeRI	GACNNNNNGTC	1 cut(s)	208
BmeT110I	CYCGRG	1 cut(s)	136
BmgT120I	GGNCC	2 cut(s)	234, 309
BmiI	GGNNCC	4 cut(s)	235, 430, 508, 849
BmrFI	CCNGG	1 cut(s)	862
BmsI	GCATC	2 cut(s)	50, 644
BpiI	GAAGAC	1 cut(s)	984
BpmI	CTGGAG	1 cut(s)	533
Bpu10I	CCTNAGC	1 cut(s)	1080
BpuEI	CTTGAG	1 cut(s)	924
BpuMI	CCSGG	1 cut(s)	862
Bsa29I	ATCGAT	1 cut(s)	1126
BsaAI	YACGTR	1 cut(s)	1181
BsaHI	GRCGYC	3 cut(s)	345, 429, 507
BsaI	GGTCTC	1 cut(s)	195
BsaJI	CCNNGG	6 cut(s)	135, 154, 173, 723, 843, 1222
BsaWI	WCCGGW	2 cut(s)	686, 1163
BsaXI	ACNNNNNCTCC	8 cut(s)	52, 82, 766, 796, 809, 839, 839, 869
Bse118I	RCCGGY	2 cut(s)	850, 1163
Bse1I	ACTGG	2 cut(s)	601, 960
Bse3DI	GCAATG	1 cut(s)	150
BseAI	TCCGGA	1 cut(s)	686
BseCI	ATCGAT	1 cut(s)	1126
BseDI	CCNNGG	6 cut(s)	135, 154, 173, 723, 843, 1222
BseGI	GGATG	3 cut(s)	41, 358, 952
BseMI	GCAATG	1 cut(s)	150
BseMII	CTCAG	3 cut(s)	101, 843, 1094
BseNI	ACTGG	2 cut(s)	601, 960
BsePI	GCGCGC	2 cut(s)	103, 105
BseRI	GAGGAG	3 cut(s)	774, 817, 887
BseXI	GCAGC	9 cut(s)	118, 137, 508, 1053, 1056, 1062, 1099, 1102, 1105
Bsh1236I	CGCG	6 cut(s)	105, 107, 156, 188, 294, 307
BshFI	GGCC	2 cut(s)	172, 951
BshNI	GGYRCC	2 cut(s)	428, 506
BshTI	ACCGGT	1 cut(s)	1163
BshVI	ATCGAT	1 cut(s)	1126
BsiHKCI	CYCGRG	1 cut(s)	136
BsiSI	CCGG	4 cut(s)	687, 851, 861, 1164
BslFI	GGGAC	1 cut(s)	247
BsmAI	GTCTC	3 cut(s)	108, 195, 1112
BsmFI	GGGAC	1 cut(s)	247
BsnI	GGCC	2 cut(s)	172, 951
Bso31I	GGTCTC	1 cut(s)	195
BsoBI	CYCGRG	1 cut(s)	136
Bsp13I	TCCGGA	1 cut(s)	686
Bsp143I	GATC	8 cut(s)	57, 195, 879, 925, 1123, 1127, 1175, 1214
Bsp19I	CCATGG	2 cut(s)	723, 1222
BspANI	GGCC	2 cut(s)	172, 951
BspCNI	CTCAG	3 cut(s)	102, 842, 1093
BspDI	ATCGAT	1 cut(s)	1126
BspEI	TCCGGA	1 cut(s)	686
BspFNI	CGCG	6 cut(s)	105, 107, 156, 188, 294, 307
BspHI	TCATGA	1 cut(s)	558
BspLI	GGNNCC	4 cut(s)	235, 430, 508, 849
BspPI	GGATC	3 cut(s)	874, 920, 1131
BspQI	GCTCTTC	1 cut(s)	631
BspT107I	GGYRCC	2 cut(s)	428, 506
BspTNI	GGTCTC	1 cut(s)	195
BsrBI	CCGCTC	1 cut(s)	281
BsrDI	GCAATG	1 cut(s)	150
BsrFI	RCCGGY	2 cut(s)	850, 1163
BsrI	ACTGG	2 cut(s)	601, 960
BssAI	RCCGGY	2 cut(s)	850, 1163
BssECI	CCNNGG	6 cut(s)	135, 154, 173, 723, 843, 1222
BssHII	GCGCGC	2 cut(s)	103, 105
BssMI	GATC	8 cut(s)	57, 195, 879, 925, 1123, 1127, 1175, 1214
BssNI	GRCGYC	3 cut(s)	345, 429, 507
BssSI	CACGAG	2 cut(s)	282, 1061
BssT1I	CCWWGG	3 cut(s)	173, 723, 1222
Bst2BI	CACGAG	2 cut(s)	282, 1061
Bst4CI	ACNGT	5 cut(s)	425, 462, 662, 1006, 1106
Bst6I	CTCTTC	3 cut(s)	331, 631, 762
BstACI	GRCGYC	3 cut(s)	345, 429, 507
BstAPI	GCANNNNNTGC	1 cut(s)	131
BstBAI	YACGTR	1 cut(s)	1181
BstC8I	GCNNGC	8 cut(s)	105, 107, 161, 170, 227, 262, 852, 979
BstDEI	CTNAG	4 cut(s)	110, 696, 829, 1080
BstDSI	CCRYGG	4 cut(s)	154, 723, 843, 1222
BstF5I	GGATG	3 cut(s)	41, 358, 952
BstFNI	CGCG	6 cut(s)	105, 107, 156, 188, 294, 307
BstH2I	RGCGCY	2 cut(s)	432, 510
BstKTI	GATC	8 cut(s)	60, 198, 882, 928, 1126, 1130, 1178, 1217
BstMAI	GTCTC	3 cut(s)	108, 195, 1112
BstMBI	GATC	8 cut(s)	57, 195, 879, 925, 1123, 1127, 1175, 1214
BstNSI	RCATGY	1 cut(s)	541
BstSCI	CCNGG	1 cut(s)	860
BstUI	CGCG	6 cut(s)	105, 107, 156, 188, 294, 307
BstV1I	GCAGC	9 cut(s)	118, 137, 508, 1053, 1056, 1062, 1099, 1102, 1105
BstV2I	GAAGAC	1 cut(s)	984
BstXI	CCANNNNNNTGG	1 cut(s)	1229
Bsu15I	ATCGAT	1 cut(s)	1126
BsuI	GTATCC	1 cut(s)	483
BsuRI	GGCC	2 cut(s)	172, 951
BsuTUI	ATCGAT	1 cut(s)	1126
BtgI	CCRYGG	4 cut(s)	154, 723, 843, 1222
BtgZI	GCGATG	1 cut(s)	869
BtsCI	GGATG	3 cut(s)	41, 358, 952
BtsIMutI	CAGTG	1 cut(s)	60
Cac8I	GCNNGC	8 cut(s)	105, 107, 161, 170, 227, 262, 852, 979
CaiI	CAGNNNCTG	1 cut(s)	131
CciI	TCATGA	1 cut(s)	558
Cfr10I	RCCGGY	2 cut(s)	850, 1163
Cfr13I	GGNCC	2 cut(s)	234, 309
Cfr42I	CCGCGG	1 cut(s)	157
ClaI	ATCGAT	1 cut(s)	1126
CseI	GACGC	2 cut(s)	174, 231
Csp6I	GTAC	5 cut(s)	458, 610, 693, 1015, 1161
CspAI	ACCGGT	1 cut(s)	1163
CviAII	CATG	7 cut(s)	38, 289, 538, 559, 671, 724, 1223
CviQI	GTAC	5 cut(s)	458, 610, 693, 1015, 1161
DdeI	CTNAG	4 cut(s)	110, 696, 829, 1080
DinI	GGCGCC	2 cut(s)	430, 508
DpnI	GATC	8 cut(s)	59, 197, 881, 927, 1125, 1129, 1177, 1216
DpnII	GATC	8 cut(s)	57, 195, 879, 925, 1123, 1127, 1175, 1214
DriI	GACNNNNNGTC	1 cut(s)	208
EaeI	YGGCCR	1 cut(s)	949
Eam1104I	CTCTTC	3 cut(s)	331, 631, 762
Eam1105I	GACNNNNNGTC	1 cut(s)	208
EarI	CTCTTC	3 cut(s)	331, 631, 762
EciI	GGCGGA	1 cut(s)	206
Eco130I	CCWWGG	3 cut(s)	173, 723, 1222
Eco147I	AGGCCT	1 cut(s)	172
Eco31I	GGTCTC	1 cut(s)	195
Eco47I	GGWCC	2 cut(s)	234, 309
Eco57I	CTGAAG	1 cut(s)	615
Eco72I	CACGTG	1 cut(s)	1181
Eco88I	CYCGRG	1 cut(s)	136
EcoO109I	RGGNCCY	1 cut(s)	234
EcoRI	GAATTC	1 cut(s)	451
EcoT14I	CCWWGG	3 cut(s)	173, 723, 1222
EcoT22I	ATGCAT	1 cut(s)	43
EgeI	GGCGCC	2 cut(s)	430, 508
EheI	GGCGCC	2 cut(s)	430, 508
ErhI	CCWWGG	3 cut(s)	173, 723, 1222
FaeI	CATG	7 cut(s)	41, 292, 541, 562, 674, 727, 1226
FaiI	YATR	9 cut(s)	39, 290, 539, 560, 672, 725, 767, 932, 1224
FaqI	GGGAC	1 cut(s)	247
FatI	CATG	7 cut(s)	37, 288, 537, 558, 670, 723, 1222
FauI	CCCGC	2 cut(s)	213, 342
FbaI	TGATCA	1 cut(s)	57
FblI	GTMKAC	2 cut(s)	321, 1168
FokI	GGATG	3 cut(s)	28, 345, 939
FspBI	CTAG	1 cut(s)	444
GsuI	CTGGAG	1 cut(s)	533
HaeII	RGCGCY	2 cut(s)	432, 510
HaeIII	GGCC	2 cut(s)	172, 951
HapII	CCGG	4 cut(s)	687, 851, 861, 1164
HgaI	GACGC	2 cut(s)	174, 231
Hin1I	GRCGYC	3 cut(s)	345, 429, 507
Hin1II	CATG	7 cut(s)	41, 292, 541, 562, 674, 727, 1226
HincII	GTYRAC	3 cut(s)	81, 646, 665
HindII	GTYRAC	3 cut(s)	81, 646, 665
HinfI	GANTC	2 cut(s)	937, 1033
HpaII	CCGG	4 cut(s)	687, 851, 861, 1164
HphI	GGTGA	5 cut(s)	386, 564, 771, 889, 1122
Hpy166II	GTNNAC	6 cut(s)	81, 322, 592, 646, 665, 1169
Hpy188I	TCNGA	5 cut(s)	46, 200, 489, 832, 1010
Hpy188III	TCNNGA	5 cut(s)	550, 559, 687, 941, 1196
Hpy8I	GTNNAC	6 cut(s)	81, 322, 592, 646, 665, 1169
Hpy99I	CGWCG	7 cut(s)	168, 225, 347, 350, 353, 497, 926
HpyAV	CCTTC	3 cut(s)	247, 772, 966
HpyCH4III	ACNGT	5 cut(s)	425, 462, 662, 1006, 1106
HpyCH4IV	ACGT	2 cut(s)	345, 1180
HpyCH4V	TGCA	2 cut(s)	41, 524
HpyF3I	CTNAG	4 cut(s)	110, 696, 829, 1080
HpySE526I	ACGT	2 cut(s)	345, 1180
Hsp92I	GRCGYC	3 cut(s)	345, 429, 507
Hsp92II	CATG	7 cut(s)	41, 292, 541, 562, 674, 727, 1226
KasI	GGCGCC	2 cut(s)	428, 506
Kpn2I	TCCGGA	1 cut(s)	686
KroI	GCCGGC	1 cut(s)	850
KroNI	GCCGGC	1 cut(s)	852
Ksp22I	TGATCA	1 cut(s)	57
KspI	CCGCGG	1 cut(s)	157
Kzo9I	GATC	8 cut(s)	57, 195, 879, 925, 1123, 1127, 1175, 1214
LguI	GCTCTTC	1 cut(s)	631
LmnI	GCTCC	2 cut(s)	739, 847
Lsp1109I	GCAGC	9 cut(s)	118, 137, 508, 1053, 1056, 1062, 1099, 1102, 1105
LweI	GCATC	2 cut(s)	50, 644
MaeI	CTAG	1 cut(s)	444
MaeII	ACGT	2 cut(s)	345, 1180
MaeIII	GTNAC	4 cut(s)	182, 413, 1049, 1144
MalI	GATC	8 cut(s)	59, 197, 881, 927, 1125, 1129, 1177, 1216
MbiI	CCGCTC	1 cut(s)	281
MboI	GATC	8 cut(s)	57, 195, 879, 925, 1123, 1127, 1175, 1214
MluCI	AATT	3 cut(s)	451, 553, 1054
Mly113I	GGCGCC	2 cut(s)	429, 507
MlyI	GAGTC	2 cut(s)	931, 1042
MmeI	TCCRAC	2 cut(s)	428, 988
Mph1103I	ATGCAT	1 cut(s)	43
MroI	TCCGGA	1 cut(s)	686
MroNI	GCCGGC	1 cut(s)	850
MroXI	GAANNNNTTC	1 cut(s)	29
MslI	CAYNNNNRTG	1 cut(s)	1227
MspA1I	CMGCKG	3 cut(s)	131, 156, 1075
MspI	CCGG	4 cut(s)	687, 851, 861, 1164
MspR9I	CCNGG	1 cut(s)	862
MvnI	CGCG	6 cut(s)	105, 107, 156, 188, 294, 307
NaeI	GCCGGC	1 cut(s)	852
NarI	GGCGCC	2 cut(s)	429, 507
NciI	CCSGG	1 cut(s)	862
NcoI	CCATGG	2 cut(s)	723, 1222
NdeII	GATC	8 cut(s)	57, 195, 879, 925, 1123, 1127, 1175, 1214
NgoMIV	GCCGGC	1 cut(s)	850
NlaIII	CATG	7 cut(s)	41, 292, 541, 562, 674, 727, 1226
NlaIV	GGNNCC	4 cut(s)	235, 430, 508, 849
NmuCI	GTSAC	1 cut(s)	1144
NsiI	ATGCAT	1 cut(s)	43
NspI	RCATGY	1 cut(s)	541
PagI	TCATGA	1 cut(s)	558
PauI	GCGCGC	2 cut(s)	103, 105
PceI	AGGCCT	1 cut(s)	172
PciSI	GCTCTTC	1 cut(s)	631
PcsI	WCGNNNNNNNCGW	1 cut(s)	851
PdiI	GCCGGC	1 cut(s)	852
PdmI	GAANNNNTTC	1 cut(s)	29
PflFI	GACNNNGTC	1 cut(s)	347
PinAI	ACCGGT	1 cut(s)	1163
PleI	GAGTC	2 cut(s)	931, 1041
PluTI	GGCGCC	2 cut(s)	432, 510
PmaCI	CACGTG	1 cut(s)	1181
PmlI	CACGTG	1 cut(s)	1181
PpsI	GAGTC	2 cut(s)	931, 1041
Ppu21I	YACGTR	1 cut(s)	1181
PpuMI	RGGWCCY	1 cut(s)	234
Psp5II	RGGWCCY	1 cut(s)	234
PspCI	CACGTG	1 cut(s)	1181
PspN4I	GGNNCC	4 cut(s)	235, 430, 508, 849
PspPI	GGNCC	2 cut(s)	234, 309
PspPPI	RGGWCCY	1 cut(s)	234
PsrI	GAACNNNNNNTAC	2 cut(s)	482, 514
PstNI	CAGNNNCTG	1 cut(s)	131
PsyI	GACNNNGTC	1 cut(s)	347
PteI	GCGCGC	2 cut(s)	103, 105
RsaI	GTAC	5 cut(s)	459, 611, 694, 1016, 1162
RsaNI	GTAC	5 cut(s)	458, 610, 693, 1015, 1161
RseI	CAYNNNNRTG	1 cut(s)	1227
SacII	CCGCGG	1 cut(s)	157
SapI	GCTCTTC	1 cut(s)	631
Sau3AI	GATC	8 cut(s)	57, 195, 879, 925, 1123, 1127, 1175, 1214
Sau96I	GGNCC	2 cut(s)	234, 309
ScaI	AGTACT	1 cut(s)	694
SchI	GAGTC	2 cut(s)	931, 1042
ScrFI	CCNGG	1 cut(s)	862
SfaNI	GCATC	2 cut(s)	50, 644
SfoI	GGCGCC	2 cut(s)	430, 508
Sfr303I	CCGCGG	1 cut(s)	157
SgrBI	CCGCGG	1 cut(s)	157
SinI	GGWCC	2 cut(s)	234, 309
SmiMI	CAYNNNNRTG	1 cut(s)	1227
SmlI	CTYRAG	1 cut(s)	939
SmoI	CTYRAG	1 cut(s)	939
Sse9I	AATT	3 cut(s)	451, 553, 1054
SseBI	AGGCCT	1 cut(s)	172
SspDI	GGCGCC	2 cut(s)	428, 506
SspI	AATATT	1 cut(s)	566
SspMI	CTAG	1 cut(s)	444
StuI	AGGCCT	1 cut(s)	172
StyD4I	CCNGG	1 cut(s)	860
StyI	CCWWGG	3 cut(s)	173, 723, 1222
TaaI	ACNGT	5 cut(s)	425, 462, 662, 1006, 1106
TaiI	ACGT	2 cut(s)	348, 1183
TaqI	TCGA	3 cut(s)	240, 935, 1126
TasI	AATT	3 cut(s)	451, 553, 1054
TatI	WGTACW	1 cut(s)	692
TscAI	CASTG	1 cut(s)	67
TseFI	GTSAC	1 cut(s)	1144
TseI	GCWGC	9 cut(s)	125, 131, 521, 1066, 1069, 1075, 1087, 1090, 1093
Tsp45I	GTSAC	1 cut(s)	1144
TspDTI	ATGAA	6 cut(s)	38, 547, 575, 687, 702, 754
TspGWI	ACGGA	4 cut(s)	445, 597, 860, 1128
TspRI	CASTG	1 cut(s)	67
Tth111I	GACNNNGTC	1 cut(s)	347
VpaK11BI	GGWCC	2 cut(s)	234, 309
XapI	RAATTY	2 cut(s)	451, 553
XceI	RCATGY	1 cut(s)	541
XmiI	GTMKAC	2 cut(s)	321, 1168
XmnI	GAANNNNTTC	1 cut(s)	29
XspI	CTAG	1 cut(s)	444
ZraI	GACGTC	1 cut(s)	346
ZrmI	AGTACT	1 cut(s)	694
Zsp2I	ATGCAT	1 cut(s)	43

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.