Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0007900

Physical Location & Seq

Genomic Coordinates Forward (+)

6323097 .. 6327434

Gene Structure 4338 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1920 bp

ATGCGTAGAAGAGAGAGACAGAGAGAGAGAGACAGAACCAGAACGCGCCACATGGAAGACCAAATCAAAGATGAGTTCCACCGGAGCGGCTTCACTTTCGACGACGAGAAGGAAATCCTCAAAAAATGTGCTACATTCTGCATAAATTTCAATCTTTCGCCTTCTGATCTCGTTACGAGCTGGGAAATTTACTACCTAAACGGGCAAATGAATGGAACGGTGGTATGTGATGCGGAAATGGATGGGTTTTTAGGGTATTTGCAAGAGCAGAAGAAAGTAGCCATTGAAGAACAGGAGCCCAATCTGCATATCTACTCAAGCAGAGATGTGGATATGATTCTGAATGATGAGGATGTAGATATAAAGGAAGAGGTTCCTAGCACTCCACCATCCAAATCCCAGAACCGTTACTCAGAGCCATCTGACTCAACGCCACAAACAATTGGGAACGTTCCTTCTTCTGGAAAACCATCAAGACTTGTAACTCCTTTTGGGCAACGAACAGACAAGTTTGTTGTGAAATTCAGCATTGATAATAAGCCAACTATAGAGAACGGGGAGAAGGAACATGATAATGAGAATGATGAGGATGATGAAATTATCAGGAAGGTTAAGCCTGGAAAGCGGTGTTCTTTAGTAGTCCATGGTTCAGGACCCGAAATAGGTTGTAGGTTTATGTATGATAGGATTGAAGATAGGTTTAACGCACTTGAAAACCGAATTAGAAAGTATGCGACTGCATTAGTTTCATCTGGGCAGTATGAGGAACCACTGGACCCTACAGTTTCGTCTCAGAAAACTATATTTACTGCTGGCATGGTCTGTTGTGACGGAGAGGGCCATTTGAACGACAAATCAACCTTGTTGCAGAGCAGCGCAGAGCATTCTGGAGGTCAACGTGTTCGTATGGAATTACAAAATTTGAGCCAATTTTCAATCTTTCCAGGCCAGATAATCGGTGTCGAAGGGACCAATCCTAGTGGACACTGCTTCATTGCTTCAAAACTGGTAGATTCAGTCCCTCTACCTGTTGCTGCTGATGCAAACTCGCCTCCTGCTAAAAAGCAGGCTTTGGATCAGGAGATTTTGCCAACTGAACTTTCCAGTAAACAACCAGAGCTATCAGTGATCATCGCATCAGGCCCTTTTACCACAACAGACAACTTATTGTTTGAGCCTCTGAGAGAGCTTCTAGCATATGCAAGCAAAAAGCTGCCTCAGTTGCTTATATTGCTAGGACCATTTATTGATTCTGAACATACAGAAATTAAGAAAGGAACCGTCGACAGGAGCTTTGATGAAATATTCCAAACTGAAATTCTTAGAAGGTTGCAAGATCATGTCGAATGCATAGGTTCGCATGCGCGCATTGTTCTTGTACCATCTATACGGGATGCTAACCATGATTTTGTATTCCCTCAGCCTGCCTTTGATATTCACCCACCTGATCTCAAGCATCAGATAAAAAGTCTCACGAATCCAGGGATTTTCGAGGCAGATCAGGTCAAGATAGGTTGCTGCTCTGTGGATGTTCTCAAACACCTTAGTGCAGAGGAGATCTCACGCGTTCCGAAGGATGGAAAACCCAGTGACCGCTTGAGTAGACTTGCAAACCATATAATTAGCCAACGCAGCTTTTATCCTTTGTATCCACCTGCAGAAGACATTCCACTGGATTTTTCACTTGTTCCTCAAGCTCTAAACATCTCATTGATCCCAAATATTCTCATCCTACCCTCTGACGTGAAGTATTTTGTGAAGGTACTGTCTATTGGTGAAAGAGGTGAAGAAAAAGAGCAGTTAAAATGCATTTGTGTCAATCCGGGAAGACTGGCCAAGGGAGAAGGAGGAGGTACTTTCGTCGAGCTTGACTACTATGGTAAGCCCGACACAACGAGTGCTTCGATTATAAGCATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000082 GO:0000228 GO:0000278 GO:0000428 GO:0000723 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005658 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0019725 GO:0022402 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032991 GO:0032993 GO:0033260 GO:0034641 GO:0034645 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044786 GO:0044843 GO:0046483 GO:0046982 GO:0046983 GO:0051276 GO:0055029 GO:0060249 GO:0061695 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576 GO:1902494 GO:1903047 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_pol_E_B Pol_alpha_B_N

Protein Analysis

640

Amino Acids

71.57

Weight (kDa)

5.26

Isoelectric Point (pI)

51.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
OB_DPOA2	PF22062	236 - 337	1.7e-23	DNA polymerase alpha subunit B, OB domain
DNA_pol_E_B	PF04042	376 - 587	6.1e-48	DNA polymerase alpha/epsilon subunit B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67630
fragaria_vesca	FvH4_4g24380 FvH4_4g24380 FvH4_4g24380 FvH4_4g24380 FvH4_4g24380 FvH4_4g24380 FvH4_4g24380
malus_domestica	MD13G1111800.v1.1 MD16G1111400.v1.1
prunus_persica	Prupe.1G233700_v2.0.a1 Prupe.1G233700_v2.0.a1
pyrus_communis	pycom13g09720 pycom16g09440
rosa_chinensis	RchiOBHm_Chr4g0431221
rosa_laevigata	RLG00000006918
rosa_multiflora	Rmu_sc0005258.1_g000007
rosa_roxburghii	Rroxscaffold_5G00372760
rosa_rugosa	Rorug04G0249200 Rorug04G0249200
rosa_samantha	Rh4BG312700 Rh4CG329000 Rh4DG309300
rosa_wichuraiana	Rw4G026540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1910
AarI	CACCTGC	1 cut(s)	1663
Acc36I	ACCTGC	1 cut(s)	1663
AccBSI	CCGCTC	1 cut(s)	87
AccI	GTMKAC	2 cut(s)	1284, 1603
AccII	CGCG	3 cut(s)	46, 1366, 1566
AciI	CCGC	4 cut(s)	87, 233, 625, 1594
AclI	AACGTT	1 cut(s)	450
AclWI	GGATC	2 cut(s)	1083, 1708
AcoI	YGGCCR	1 cut(s)	1833
AcsI	RAATTY	5 cut(s)	145, 186, 521, 919, 1317
AfaI	GTAC	3 cut(s)	1380, 1764, 1855
AfiI	CCNNNNNNNGG	4 cut(s)	461, 662, 1287, 1577
AflIII	ACRYGT	2 cut(s)	898, 1564
AgsI	TTSAA	7 cut(s)	151, 287, 692, 713, 847, 936, 1002
AjiI	CACGTC	1 cut(s)	1744
AjnI	CCWGG	3 cut(s)	616, 943, 1480
AjuI	GAANNNNNNNTTGG	4 cut(s)	1084, 1116, 1302, 1334
AleI	CACNNNNGTG	1 cut(s)	1545
AluBI	AGCT	8 cut(s)	180, 1120, 1189, 1213, 1293, 1635, 1697, 1867
AluI	AGCT	8 cut(s)	180, 1120, 1189, 1213, 1293, 1635, 1697, 1867
Alw26I	GTCTC	4 cut(s)	10, 24, 795, 1475
AlwI	GGATC	2 cut(s)	1083, 1708
AoxI	GGCC	4 cut(s)	838, 946, 1141, 1833
ApeKI	GCWGC	5 cut(s)	873, 1034, 1213, 1518, 1632
ApoI	RAATTY	5 cut(s)	145, 186, 521, 919, 1317
ArsI	GACNNNNNNTTYG	2 cut(s)	1277, 1309
Asp700I	GAANNNNTTC	2 cut(s)	372, 1665
AspLEI	GCGC	4 cut(s)	48, 878, 1366, 1368
AspS9I	GGNCC	6 cut(s)	653, 775, 838, 969, 1142, 1238
AsuC2I	CCSGG	1 cut(s)	1824
AsuHPI	GGTGA	3 cut(s)	1430, 1787, 1796
AvaII	GGWCC	4 cut(s)	653, 775, 969, 1238
BaeI	ACNNNNGTAYC	2 cut(s)	1845, 1878
BalI	TGGCCA	1 cut(s)	1835
BanII	GRGCYC	1 cut(s)	300
BbsI	GAAGAC	3 cut(s)	63, 1668, 1834
BbvCI	CCTCAGC	1 cut(s)	1419
BbvI	GCAGC	5 cut(s)	885, 1021, 1200, 1505, 1644
BccI	CCATC	6 cut(s)	236, 397, 427, 478, 1390, 1571
BciT130I	CCWGG	3 cut(s)	618, 945, 1482
BciVI	GTATCC	1 cut(s)	1659
BclI	TGATCA	1 cut(s)	1128
BcnI	CCSGG	1 cut(s)	1824
BcoDI	GTCTC	4 cut(s)	10, 24, 795, 1475
BfaI	CTAG	4 cut(s)	378, 978, 1193, 1235
BfmI	CTRYAG	3 cut(s)	546, 780, 1656
BfuAI	ACCTGC	1 cut(s)	1663
BfuI	GTATCC	1 cut(s)	1659
BglII	AGATCT	1 cut(s)	1557
BisI	GCNGC	6 cut(s)	88, 874, 1035, 1214, 1519, 1633
BlsI	GCNGC	6 cut(s)	89, 875, 1036, 1215, 1520, 1634
Bme1390I	CCNGG	4 cut(s)	618, 945, 1482, 1824
Bme18I	GGWCC	4 cut(s)	653, 775, 969, 1238
BmgBI	CACGTC	1 cut(s)	1744
BmgT120I	GGNCC	6 cut(s)	653, 775, 838, 969, 1142, 1238
BmiI	GGNNCC	7 cut(s)	297, 375, 655, 768, 777, 970, 1279
BmrFI	CCNGG	4 cut(s)	618, 945, 1482, 1824
BmrI	ACTGGG	1 cut(s)	1581
BmsI	GCATC	5 cut(s)	220, 1030, 1145, 1384, 1465
BmuI	ACTGGG	1 cut(s)	1581
BpiI	GAAGAC	3 cut(s)	63, 1668, 1834
BplI	GAGNNNNNCTC	2 cut(s)	1544, 1576
BpmI	CTGGAG	1 cut(s)	909
Bpu10I	CCTNAGC	1 cut(s)	1419
BpuEI	CTTGAG	4 cut(s)	301, 1436, 1618, 1677
BpuMI	CCSGG	1 cut(s)	1824
BsaJI	CCNNGG	3 cut(s)	643, 1481, 1836
BsaWI	WCCGGW	1 cut(s)	81
Bsc4I	CCNNNNNNNGG	4 cut(s)	461, 662, 1287, 1577
Bse1I	ACTGG	6 cut(s)	777, 1011, 1104, 1587, 1677, 1836
Bse3DI	GCAATG	1 cut(s)	993
BseBI	CCWGG	3 cut(s)	618, 945, 1482
BseDI	CCNNGG	3 cut(s)	643, 1481, 1836
BseGI	GGATG	8 cut(s)	247, 358, 389, 595, 1399, 1534, 1582, 1728
BseLI	CCNNNNNNNGG	4 cut(s)	461, 662, 1287, 1577
BseMI	GCAATG	1 cut(s)	993
BseMII	CTCAG	5 cut(s)	426, 806, 1172, 1232, 1433
BseNI	ACTGG	6 cut(s)	777, 1011, 1104, 1587, 1677, 1836
BsePI	GCGCGC	1 cut(s)	1364
BseRI	GAGGAG	2 cut(s)	1568, 1863
BseXI	GCAGC	5 cut(s)	885, 1021, 1200, 1505, 1644
BseYI	CCCAGC	1 cut(s)	180
BsgI	GTGCAG	1 cut(s)	1569
Bsh1236I	CGCG	3 cut(s)	46, 1366, 1566
BshFI	GGCC	4 cut(s)	840, 948, 1143, 1835
BsiSI	CCGG	2 cut(s)	82, 1823
BslFI	GGGAC	2 cut(s)	982, 1004
BslI	CCNNNNNNNGG	4 cut(s)	461, 662, 1287, 1577
BsmAI	GTCTC	4 cut(s)	10, 24, 795, 1475
BsmBI	CGTCTC	1 cut(s)	795
BsmFI	GGGAC	2 cut(s)	982, 1004
BsmI	GAATGC	2 cut(s)	883, 1352
BsnI	GGCC	4 cut(s)	840, 948, 1143, 1835
Bsp1286I	GDGCHC	1 cut(s)	300
Bsp143I	GATC	8 cut(s)	166, 1075, 1128, 1336, 1447, 1498, 1557, 1713
Bsp19I	CCATGG	1 cut(s)	643
BspACI	CCGC	4 cut(s)	87, 233, 625, 1594
BspANI	GGCC	4 cut(s)	840, 948, 1143, 1835
BspCNI	CTCAG	5 cut(s)	425, 805, 1173, 1231, 1432
BspFNI	CGCG	3 cut(s)	46, 1366, 1566
BspLI	GGNNCC	7 cut(s)	297, 375, 655, 768, 777, 970, 1279
BspMAI	CTGCAG	1 cut(s)	1660
BspMI	ACCTGC	1 cut(s)	1663
BspPI	GGATC	2 cut(s)	1083, 1708
BsrBI	CCGCTC	1 cut(s)	87
BsrDI	GCAATG	1 cut(s)	993
BsrI	ACTGG	6 cut(s)	777, 1011, 1104, 1587, 1677, 1836
BssECI	CCNNGG	3 cut(s)	643, 1481, 1836
BssHII	GCGCGC	1 cut(s)	1364
BssMI	GATC	8 cut(s)	166, 1075, 1128, 1336, 1447, 1498, 1557, 1713
BssT1I	CCWWGG	2 cut(s)	643, 1836
Bst2UI	CCWGG	3 cut(s)	618, 945, 1482
Bst4CI	ACNGT	5 cut(s)	220, 407, 784, 1282, 1767
Bst6I	CTCTTC	2 cut(s)	4, 363
BstC8I	GCNNGC	6 cut(s)	814, 1068, 1204, 1362, 1366, 1425
BstDEI	CTNAG	7 cut(s)	412, 792, 1181, 1218, 1322, 1419, 1544
BstDSI	CCRYGG	1 cut(s)	643
BstF5I	GGATG	8 cut(s)	247, 358, 389, 595, 1399, 1534, 1582, 1728
BstFNI	CGCG	3 cut(s)	46, 1366, 1566
BstHHI	GCGC	4 cut(s)	48, 878, 1366, 1368
BstKTI	GATC	8 cut(s)	169, 1078, 1131, 1339, 1450, 1501, 1560, 1716
BstMAI	GTCTC	4 cut(s)	10, 24, 795, 1475
BstMBI	GATC	8 cut(s)	166, 1075, 1128, 1336, 1447, 1498, 1557, 1713
BstMWI	GCNNNNNNNGC	6 cut(s)	304, 622, 1040, 1222, 1231, 1632
BstNI	CCWGG	3 cut(s)	618, 945, 1482
BstNSI	RCATGY	1 cut(s)	1364
BstSCI	CCNGG	4 cut(s)	616, 943, 1480, 1822
BstSFI	CTRYAG	3 cut(s)	546, 780, 1656
BstUI	CGCG	3 cut(s)	46, 1366, 1566
BstV1I	GCAGC	5 cut(s)	885, 1021, 1200, 1505, 1644
BstV2I	GAAGAC	3 cut(s)	63, 1668, 1834
BstX2I	RGATCY	1 cut(s)	1557
BstYI	RGATCY	1 cut(s)	1557
BsuI	GTATCC	1 cut(s)	1659
BsuRI	GGCC	4 cut(s)	840, 948, 1143, 1835
BtgI	CCRYGG	1 cut(s)	643
BtgZI	GCGATG	1 cut(s)	1117
BtrI	CACGTC	1 cut(s)	1744
BtsCI	GGATG	8 cut(s)	247, 358, 389, 595, 1399, 1534, 1582, 1728
BtsI	GCAGTG	1 cut(s)	985
BtsIMutI	CAGTG	5 cut(s)	770, 985, 1131, 1594, 1670
BveI	ACCTGC	1 cut(s)	1663
Cac8I	GCNNGC	6 cut(s)	814, 1068, 1204, 1362, 1366, 1425
CfoI	GCGC	4 cut(s)	48, 878, 1366, 1368
Cfr13I	GGNCC	6 cut(s)	653, 775, 838, 969, 1142, 1238
Csp6I	GTAC	3 cut(s)	1379, 1763, 1854
CspCI	CAANNNNNGTGG	2 cut(s)	961, 996
CviAII	CATG	7 cut(s)	52, 569, 644, 817, 1340, 1361, 1403
CviQI	GTAC	3 cut(s)	1379, 1763, 1854
DdeI	CTNAG	7 cut(s)	412, 792, 1181, 1218, 1322, 1419, 1544
DpnI	GATC	8 cut(s)	168, 1077, 1130, 1338, 1449, 1500, 1559, 1715
DpnII	GATC	8 cut(s)	166, 1075, 1128, 1336, 1447, 1498, 1557, 1713
EaeI	YGGCCR	1 cut(s)	1833
Eam1104I	CTCTTC	2 cut(s)	4, 363
EarI	CTCTTC	2 cut(s)	4, 363
Eco130I	CCWWGG	2 cut(s)	643, 1836
Eco24I	GRGCYC	1 cut(s)	300
Eco47I	GGWCC	4 cut(s)	653, 775, 969, 1238
EcoO109I	RGGNCCY	2 cut(s)	653, 1142
EcoRII	CCWGG	3 cut(s)	616, 943, 1480
EcoT14I	CCWWGG	2 cut(s)	643, 1836
EcoT22I	ATGCAT	2 cut(s)	1352, 1811
EcoT38I	GRGCYC	1 cut(s)	300
ErhI	CCWWGG	2 cut(s)	643, 1836
Esp3I	CGTCTC	1 cut(s)	795
FaeI	CATG	7 cut(s)	55, 572, 647, 820, 1343, 1364, 1406
FaqI	GGGAC	2 cut(s)	982, 1004
FatI	CATG	7 cut(s)	51, 568, 643, 816, 1339, 1360, 1402
FauNDI	CATATG	1 cut(s)	1198
FbaI	TGATCA	1 cut(s)	1128
FblI	GTMKAC	2 cut(s)	1284, 1603
Fnu4HI	GCNGC	6 cut(s)	88, 874, 1035, 1214, 1519, 1633
FokI	GGATG	8 cut(s)	254, 365, 376, 602, 1406, 1541, 1589, 1715
FriOI	GRGCYC	1 cut(s)	300
Fsp4HI	GCNGC	6 cut(s)	88, 874, 1035, 1214, 1519, 1633
FspBI	CTAG	4 cut(s)	378, 978, 1193, 1235
GlaI	GCGC	4 cut(s)	47, 877, 1365, 1367
GluI	GCNGC	6 cut(s)	88, 874, 1035, 1214, 1519, 1633
GsaI	CCCAGC	1 cut(s)	184
GsuI	CTGGAG	1 cut(s)	909
HaeIII	GGCC	4 cut(s)	840, 948, 1143, 1835
HapII	CCGG	2 cut(s)	82, 1823
HhaI	GCGC	4 cut(s)	48, 878, 1366, 1368
Hin1II	CATG	7 cut(s)	55, 572, 647, 820, 1343, 1364, 1406
Hin6I	GCGC	4 cut(s)	46, 876, 1364, 1366
HinP1I	GCGC	4 cut(s)	46, 876, 1364, 1366
HincII	GTYRAC	2 cut(s)	896, 1285
HindII	GTYRAC	2 cut(s)	896, 1285
HinfI	GANTC	5 cut(s)	337, 425, 1013, 1250, 1477
HpaII	CCGG	2 cut(s)	82, 1823
HphI	GGTGA	3 cut(s)	1430, 1787, 1796
Hpy166II	GTNNAC	5 cut(s)	896, 983, 1109, 1285, 1604
Hpy188III	TCNNGA	8 cut(s)	462, 474, 604, 651, 888, 1079, 1474, 1507
Hpy8I	GTNNAC	5 cut(s)	896, 983, 1109, 1285, 1604
Hpy99I	CGWCG	4 cut(s)	104, 107, 1286, 1865
HpyCH4III	ACNGT	5 cut(s)	220, 407, 784, 1282, 1767
HpyCH4IV	ACGT	3 cut(s)	450, 898, 1743
HpyF10VI	GCNNNNNNNGC	6 cut(s)	304, 622, 1040, 1222, 1231, 1632
HpyF3I	CTNAG	7 cut(s)	412, 792, 1181, 1218, 1322, 1419, 1544
HpySE526I	ACGT	3 cut(s)	450, 898, 1743
Hsp92II	CATG	7 cut(s)	55, 572, 647, 820, 1343, 1364, 1406
HspAI	GCGC	4 cut(s)	46, 876, 1364, 1366
Ksp22I	TGATCA	1 cut(s)	1128
Kzo9I	GATC	8 cut(s)	166, 1075, 1128, 1336, 1447, 1498, 1557, 1713
LmnI	GCTCC	3 cut(s)	84, 295, 1290
Lsp1109I	GCAGC	5 cut(s)	885, 1021, 1200, 1505, 1644
LweI	GCATC	5 cut(s)	220, 1030, 1145, 1384, 1465
MaeI	CTAG	4 cut(s)	378, 978, 1193, 1235
MaeII	ACGT	3 cut(s)	450, 898, 1743
MaeIII	GTNAC	5 cut(s)	172, 407, 481, 827, 1589
MalI	GATC	8 cut(s)	168, 1077, 1130, 1338, 1449, 1500, 1559, 1715
MbiI	CCGCTC	1 cut(s)	87
MboI	GATC	8 cut(s)	166, 1075, 1128, 1336, 1447, 1498, 1557, 1713
MfeI	CAATTG	1 cut(s)	441
MflI	RGATCY	1 cut(s)	1557
MhlI	GDGCHC	1 cut(s)	300
MlsI	TGGCCA	1 cut(s)	1835
MluI	ACGCGT	1 cut(s)	1564
MluNI	TGGCCA	1 cut(s)	1835
MlyI	GAGTC	1 cut(s)	419
Mox20I	TGGCCA	1 cut(s)	1835
Mph1103I	ATGCAT	2 cut(s)	1352, 1811
MroXI	GAANNNNTTC	2 cut(s)	372, 1665
MscI	TGGCCA	1 cut(s)	1835
MseI	TTAA	4 cut(s)	612, 702, 1269, 1802
MslI	CAYNNNNRTG	2 cut(s)	573, 1545
Msp20I	TGGCCA	1 cut(s)	1835
MspI	CCGG	2 cut(s)	82, 1823
MspR9I	CCNGG	4 cut(s)	618, 945, 1482, 1824
MunI	CAATTG	1 cut(s)	441
Mva1269I	GAATGC	2 cut(s)	883, 1352
MvaI	CCWGG	3 cut(s)	618, 945, 1482
MvnI	CGCG	3 cut(s)	46, 1366, 1566
MwoI	GCNNNNNNNGC	6 cut(s)	304, 622, 1040, 1222, 1231, 1632
NciI	CCSGG	1 cut(s)	1824
NcoI	CCATGG	1 cut(s)	643
NdeI	CATATG	1 cut(s)	1198
NdeII	GATC	8 cut(s)	166, 1075, 1128, 1336, 1447, 1498, 1557, 1713
NlaIII	CATG	7 cut(s)	55, 572, 647, 820, 1343, 1364, 1406
NlaIV	GGNNCC	7 cut(s)	297, 375, 655, 768, 777, 970, 1279
NmuCI	GTSAC	2 cut(s)	827, 1589
NsiI	ATGCAT	2 cut(s)	1352, 1811
NspI	RCATGY	1 cut(s)	1364
OliI	CACNNNNGTG	1 cut(s)	1545
PaeI	GCATGC	1 cut(s)	1364
PaqCI	CACCTGC	1 cut(s)	1663
PauI	GCGCGC	1 cut(s)	1364
PcsI	WCGNNNNNNNCGW	1 cut(s)	1901
PctI	GAATGC	2 cut(s)	883, 1352
PdmI	GAANNNNTTC	2 cut(s)	372, 1665
PfeI	GAWTC	4 cut(s)	337, 1013, 1250, 1477
PfoI	TCCNGGA	1 cut(s)	1822
PkrI	GCNGC	6 cut(s)	89, 875, 1036, 1215, 1520, 1634
PleI	GAGTC	1 cut(s)	419
PpsI	GAGTC	1 cut(s)	419
PpuMI	RGGWCCY	1 cut(s)	653
PsiI	TTATAA	1 cut(s)	1910
Psp1406I	AACGTT	1 cut(s)	450
Psp5II	RGGWCCY	1 cut(s)	653
Psp6I	CCWGG	3 cut(s)	616, 943, 1480
PspFI	CCCAGC	1 cut(s)	180
PspGI	CCWGG	3 cut(s)	616, 943, 1480
PspN4I	GGNNCC	7 cut(s)	297, 375, 655, 768, 777, 970, 1279
PspPI	GGNCC	6 cut(s)	653, 775, 838, 969, 1142, 1238
PspPPI	RGGWCCY	1 cut(s)	653
PstI	CTGCAG	1 cut(s)	1660
PsuI	RGATCY	1 cut(s)	1557
PteI	GCGCGC	1 cut(s)	1364
RsaI	GTAC	3 cut(s)	1380, 1764, 1855
RsaNI	GTAC	3 cut(s)	1379, 1763, 1854
RseI	CAYNNNNRTG	2 cut(s)	573, 1545
SalI	GTCGAC	1 cut(s)	1283
SaqAI	TTAA	4 cut(s)	612, 702, 1269, 1802
SatI	GCNGC	6 cut(s)	88, 874, 1035, 1214, 1519, 1633
Sau3AI	GATC	8 cut(s)	166, 1075, 1128, 1336, 1447, 1498, 1557, 1713
Sau96I	GGNCC	6 cut(s)	653, 775, 838, 969, 1142, 1238
SchI	GAGTC	1 cut(s)	419
ScrFI	CCNGG	4 cut(s)	618, 945, 1482, 1824
SduI	GDGCHC	1 cut(s)	300
SfaNI	GCATC	5 cut(s)	220, 1030, 1145, 1384, 1465
SfcI	CTRYAG	3 cut(s)	546, 780, 1656
SinI	GGWCC	4 cut(s)	653, 775, 969, 1238
SmiMI	CAYNNNNRTG	2 cut(s)	573, 1545
SmlI	CTYRAG	4 cut(s)	316, 1451, 1597, 1692
SmoI	CTYRAG	4 cut(s)	316, 1451, 1597, 1692
SphI	GCATGC	1 cut(s)	1364
SsiI	CCGC	4 cut(s)	87, 233, 625, 1594
SspI	AATATT	2 cut(s)	1305, 1723
SspMI	CTAG	4 cut(s)	378, 978, 1193, 1235
StyD4I	CCNGG	4 cut(s)	616, 943, 1480, 1822
StyI	CCWWGG	2 cut(s)	643, 1836
TaaI	ACNGT	5 cut(s)	220, 407, 784, 1282, 1767
TaiI	ACGT	3 cut(s)	453, 901, 1746
TaqI	TCGA	7 cut(s)	99, 963, 1284, 1344, 1491, 1863, 1904
TauI	GCSGC	1 cut(s)	90
TfiI	GAWTC	4 cut(s)	337, 1013, 1250, 1477
Tru1I	TTAA	4 cut(s)	612, 702, 1269, 1802
Tru9I	TTAA	4 cut(s)	612, 702, 1269, 1802
TscAI	CASTG	5 cut(s)	777, 992, 1131, 1594, 1677
TseFI	GTSAC	2 cut(s)	827, 1589
TseI	GCWGC	5 cut(s)	873, 1034, 1213, 1518, 1632
Tsp45I	GTSAC	2 cut(s)	827, 1589
TspDTI	ATGAA	5 cut(s)	224, 609, 738, 982, 1314
TspGWI	ACGGA	1 cut(s)	846
TspRI	CASTG	5 cut(s)	777, 992, 1131, 1594, 1677
VpaK11BI	GGWCC	4 cut(s)	653, 775, 969, 1238
XapI	RAATTY	5 cut(s)	145, 186, 521, 919, 1317
XceI	RCATGY	1 cut(s)	1364
XmiI	GTMKAC	2 cut(s)	1284, 1603
XmnI	GAANNNNTTC	2 cut(s)	372, 1665
XspI	CTAG	4 cut(s)	378, 978, 1193, 1235
Zsp2I	ATGCAT	2 cut(s)	1352, 1811

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.