Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0011670

Physical Location & Seq

Genomic Coordinates Reverse (-)

7977650 .. 7981907

Gene Structure 4258 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2895 bp

ATGAACGGTCATCACCACGAGGAAGGGATGCTTACTTCCTCGGACTACAACTCCGACACCGAGTCCACTCAGTCCACCGGGCCCGGCGGCAGCATCGAGCTCGAAAGACGAGCTTTCAGCGGCCCCCTGGGAACGCCTAACAGCGGCAACCACACCCCGACCACGTCCTCCAATTCCAACCGGGCGTGGAGGCGGATTAAGAGCGCCAAGTTCGACAAGTCCGGCGGTACGGATAAGAGTACTGGTAATACTTCCGACCAAAACGACGATGATACTTACGTGGAGATCACTCTCGACATCCGAGACGACTCCGTCGCCGTCCACAGCGTCCAGGCAGCCGGCGGAGCCAGTAACGAAGATCCCGAGCTGGCTCTGCTCGCCAAGCGGACGCTGGAGGGGAAGAAGTCGTCGTCTTTCCGGTCGTCGCTTTTGCGGAACACGTCGTCGCACATCCGGCAGGTCTCTCAGGAGCTGAAGCGGCTGGCTTCCTTCAGCAAGAGGCCCTCCACGAAGCGGTTCGATCGGACCAAGTCAGCAACCGCCTACGCCTTGAAGAGCCTCAAGTTCATCACCACCAAGACCGGCACCGGCGCCACCTCCGGCTGGGCCGCCGTAGACAAGCGCTACGACGAGCTCACTGCCAAATCCAACTGGCTCCTCCCCTCTTCCCTCTTTGGCGAATGCATAGGTATGAACAAGGAGTCCAAGGAGTTTGCCGGAGAGCTTTTCCGGGCACTTGCTCGGAGGCGGAACATAACCGGCGATATCATCAACAAGGCGCAACTTAGAGAATTCTGGGAGCAAATCTCCGACGAAAGTTTTGATTCCAGACTCCAAACATTCTTTGACATGGTGGATAGAGACGCCGATGGAAGAATCACTGAAGAAGAAGTCACAGAGATTATAAGCATGAGTGCTTCTGCAAACAAGCTCTCAAACATTCAGAAACAAGCAAAGGAATATGCAGCTCTGATCATGGAAGAACTAGACCCGGACAATGCCGGCTACATCATGGTTCAAAATTTGGAAACTCTGTTGCTCCAAGCTCCGGTTGGCCAATCTGTGAGCGTAAACGAAAGCCGGGTTTTGAGCCAGCTTCTGAGTCAGAAGCTGAAGCCGACGCAGGAGAACAACCCGATCCGAAGATGCTACCAGAGAACTAAGTACTTCTTCCTTGATAACTGGCAGAGAGTCTGGGTTATGATGCTCTGGCTCGGCATAGTTTCAGGTCTGTTTGTGTACAAGTTTGTGCAGTACCGCGAAAGAGAGGAGGCATTCGAGGTGATGGGATATTGTGTCTGCATTGCCAAAGGAGGAGCTGAGACTCTCAAGTTTAACATGGCCTTGATCCTGCTTCCCGTCTGCCGGAACACAATTACTTGGCTCCGAAACAAGACCAAATTAGGCGTTGCCGTCCCGTTTGATGACAACTTAAATTTCCACAAGGTCATTGCGGTTGGAATCATTGTTGGAGTTGGACTACATGTGGGTGCGCATTTGACGTGCGATTTCCCACGGATTATACACGCGACCGAAGATGAGTACGAGGACTTGAAACCTTACTTCGGCGAGGACCAGCCTGGGAACTACTGGTGGTTCGTGAAGGGAGTGGAAGGGTGGTCTGGTATTTTGATGGTGGTATTGATGGCCATAGCCTTCACGTTGGCAACTCCTTGGTTCAGAAGAAACAAGCTCAACTTGCCAAAGCCGCTCAAGAAGCTCACTGGCTTCAACGCCTTCTGGTATTCCCACCACCTGTTTGTCATTGTCTATGCTCTCCTCATTGTCCATGGCATCAAGCTCTACCTCACCAAGGAATGGTATCATAAAACGACATGGATGTATTTGGCGGTTCCTGTCGTCCTCTACGCTTGCGAAAGGTTGATTAGAGCTTTTAGATCAAGCATCAAGCCAGTCAAGATTCTCAAGGTTGCTGTTTATCCTGGAAATGTTCTTGCACTGCACATGTCAAAGCCTCAGGGCTTCAAATACAAGAGCGGGCAGTACATGTTTGTCAACTGCGCTGCGGTTTCTCCTTTCGAATGGCACCCGTTCTCCATTACCTCATCACCTGGAGATGACTACCTAAGCGTCCACATTCGAACACTGGGCGATTGGACGCGGCAGCTCAAAACTGTTTTCTCCGAGGTATGTCAGCCACCGACTGGTGGGAAGAGTGGCCTACTCAGAGCTGATATGCAAGGAGGAAACAATCCTAGCTTCCCTAAGATACTGATAGATGGTCCATACGGAGCACCGGCACAGGACTACAAGAAATACGACGTTGTATTGCTTGTAGGGCTCGGAATCGGAGCAACTCCCATGGTGAGCATTGTGAAGGACATCATCAACAACATAAAGATGAAGAGCAGGGAAGACGATGATTCGGAGTCCGCCCTAGAAAGCGGCAGGGCCACTCCGAATGCCTCGAGCCATAACAACAGCAGCAGTGGCAAGAACAGGAGCAACAAAGGGTTCAAGACCAGGAAGGCCTACTACTATTGGGTGACGAGAGAACAAGGCTCGTTCGAGTGGTTCAAGGGGATTCTGAACGAGGTGGCGGACATGGACGAGAAGGGTGTGATCGAGATTCACAACTACTGCACCAGTGTGTACGAGGAAGGGGATGCCAGGTCAGCCCTCATTGCCATGCTCCAGTCCCTCCACCACGCCAAGAACGGCGTCGATGTGGTGTCGGGCACACGTGTCAAATCCCACTTCGCCAAGCCCAACTGGCGCCAAGTTTACAAGGATATCGCTCGCCGCCATCCGGATAGCCGAGTGGGGGTGTTTTACTGTGGGGCACCAGCACTAACAAAGGACCTCAAGGAATTGGCGTTGTACTTTTCACACAAGACCACCACGAAGTTCGACTTCCACAAAGAGAATTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009628 GO:0009987 GO:0010941 GO:0012505 GO:0016020 GO:0016174 GO:0016491 GO:0016651 GO:0023052 GO:0033500 GO:0033554 GO:0042592 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048878 GO:0050664 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055114 GO:0060548 GO:0065007 GO:0065008 GO:0071214 GO:0071470 GO:0071944 GO:0072593 GO:0098542 GO:0104004

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

965

Amino Acids

108.53

Weight (kDa)

9.04

Isoelectric Point (pI)

42.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NADPH_Ox	PF08414	172 - 276	6e-35	Respiratory burst NADPH oxidase
EF-hand_7	PF13499	276 - 340	5.8e-06	EF-hand domain pair
Ferric_reduct	PF01794	447 - 594	1.1e-12	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	638 - 754	6e-37	FAD-binding domain
NAD_binding_6	PF08030	760 - 946	1.1e-49	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011670)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47910
fragaria_vesca	FvH4_4g22100 FvH4_4g22100
malus_domestica	MD13G1134500.v1.1 MD16G1145100.v1.1
prunus_persica	Prupe.1G211000_v2.0.a1
pyrus_communis	pycom13g11730 pycom16g11470
rosa_chinensis	RchiOBHm_Chr4g0428361
rosa_laevigata	RLG00000007160
rosa_multiflora	Rmu_co8508821.1_g000001 Rmu_sc0003384.1_g000003 Rmu_sc0010192.1_g000005
rosa_roxburghii	Rroxscaffold_5G00370020
rosa_rugosa	Rorug04G0225000
rosa_samantha	Rh4AG281800 Rh4CG303200 Rh4DG284600
rosa_wichuraiana	Rw4G024470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	905
AasI	GACNNNNNNGTC	1 cut(s)	311
Acc16I	TGCGCA	1 cut(s)	1494
Acc36I	ACCTGC	1 cut(s)	448
AccB1I	GGYRCC	5 cut(s)	584, 590, 2046, 2736, 2803
AccB7I	CCANNNNNTGG	2 cut(s)	1818, 2165
AccBSI	CCGCTC	2 cut(s)	1711, 1998
AccI	GTMKAC	1 cut(s)	615
AccII	CGCG	3 cut(s)	1260, 1529, 2122
AccIII	TCCGGA	1 cut(s)	2770
AclWI	GGATC	3 cut(s)	353, 1132, 1342
AcoI	YGGCCR	2 cut(s)	1054, 1647
AcsI	RAATTY	4 cut(s)	791, 1021, 1435, 2887
AcuI	CTGAAG	4 cut(s)	475, 494, 903, 1133
AcvI	CACGTG	1 cut(s)	2705
AcyI	GRCGYC	4 cut(s)	591, 864, 2682, 2737
AfaI	GTAC	9 cut(s)	229, 241, 1166, 1241, 1256, 1544, 2006, 2615, 2843
AfeI	AGCGCT	1 cut(s)	623
AflIII	ACRYGT	6 cut(s)	438, 1483, 1965, 2007, 2702, 2704
AgsI	TTSAA	7 cut(s)	553, 1019, 1555, 1732, 1987, 2479, 2539
AhdI	GACNNNNNGTC	1 cut(s)	61
AjiI	CACGTC	3 cut(s)	165, 441, 1503
AjnI	CCWGG	7 cut(s)	126, 330, 1579, 1942, 2071, 2483, 2630
AleI	CACNNNNGTG	1 cut(s)	2609
AloI	GAACNNNNNNTCC	2 cut(s)	686, 718
Alw21I	GWGCWC	3 cut(s)	102, 636, 2257
Alw26I	GTCTC	4 cut(s)	297, 466, 855, 1316
AlwI	GGATC	3 cut(s)	353, 1132, 1342
AlwNI	CAGNNNCTG	3 cut(s)	472, 1099, 1111
Ama87I	CYCGRG	2 cut(s)	362, 2428
Aor13HI	TCCGGA	1 cut(s)	2770
Aor51HI	AGCGCT	1 cut(s)	623
ApaI	GGGCCC	1 cut(s)	84
ApeKI	GCWGC	6 cut(s)	90, 335, 965, 2024, 2125, 2445
ApoI	RAATTY	4 cut(s)	791, 1021, 1435, 2887
ArsI	GACNNNNNNTTYG	2 cut(s)	803, 835
Asp700I	GAANNNNTTC	1 cut(s)	515
AspLEI	GCGC	7 cut(s)	206, 593, 624, 781, 1495, 2024, 2739
AsuC2I	CCSGG	6 cut(s)	79, 84, 182, 731, 992, 1082
AsuHPI	GGTGA	7 cut(s)	5, 562, 1294, 1801, 2061, 2338, 2518
AsuII	TTCGAA	2 cut(s)	2040, 2101
AvaI	CYCGRG	2 cut(s)	362, 2428
AvaII	GGWCC	4 cut(s)	525, 1573, 2243, 2821
AxyI	CCTNAGG	1 cut(s)	1977
BaeGI	GKGCMC	4 cut(s)	84, 736, 2702, 2806
BaeI	ACNNNNGTAYC	2 cut(s)	264, 297
BalI	TGGCCA	2 cut(s)	1056, 1649
BanI	GGYRCC	5 cut(s)	584, 590, 2046, 2736, 2803
BanII	GRGCYC	4 cut(s)	84, 102, 636, 2304
BarI	GAAGNNNNNNTAC	2 cut(s)	2480, 2512
BauI	CACGAG	1 cut(s)	17
BbrPI	CACGTG	1 cut(s)	2705
BbsI	GAAGAC	1 cut(s)	2382
Bbv12I	GWGCWC	3 cut(s)	102, 636, 2257
BbvI	GCAGC	6 cut(s)	102, 347, 977, 2011, 2137, 2457
BccI	CCATC	6 cut(s)	863, 1279, 1627, 1639, 2234, 2775
BceAI	ACGGC	4 cut(s)	302, 596, 1397, 2695
BcgI	CGANNNNNNTGC	4 cut(s)	82, 116, 620, 654
BciT130I	CCWGG	7 cut(s)	128, 332, 1581, 1944, 2073, 2485, 2632
BclI	TGATCA	1 cut(s)	972
BcnI	CCSGG	6 cut(s)	79, 84, 182, 731, 992, 1082
BcoDI	GTCTC	4 cut(s)	297, 466, 855, 1316
BfaI	CTAG	3 cut(s)	986, 2217, 2399
BfoI	RGCGCY	4 cut(s)	207, 594, 625, 2740
BfuAI	ACCTGC	1 cut(s)	448
BglI	GCCNNNNNGGC	1 cut(s)	2734
BmcAI	AGTACT	2 cut(s)	241, 1166
Bme18I	GGWCC	4 cut(s)	525, 1573, 2243, 2821
BmeRI	GACNNNNNGTC	1 cut(s)	61
BmeT110I	CYCGRG	2 cut(s)	362, 2428
BmgBI	CACGTC	3 cut(s)	165, 441, 1503
BmrI	ACTGGG	1 cut(s)	2117
BmsI	GCATC	7 cut(s)	18, 102, 1136, 1194, 1803, 1914, 2617
BmuI	ACTGGG	1 cut(s)	2117
BpiI	GAAGAC	1 cut(s)	2382
BplI	GAGNNNNNCTC	2 cut(s)	791, 823
BpmI	CTGGAG	3 cut(s)	413, 2094, 2639
Bpu10I	CCTNAGC	1 cut(s)	2087
Bpu14I	TTCGAA	2 cut(s)	2040, 2101
BpuEI	CTTGAG	5 cut(s)	545, 1313, 1697, 1910, 2810
BpuMI	CCSGG	6 cut(s)	79, 84, 182, 731, 992, 1082
BsaAI	YACGTR	2 cut(s)	280, 2705
BsaHI	GRCGYC	4 cut(s)	591, 864, 2682, 2737
BsaI	GGTCTC	1 cut(s)	466
BsaWI	WCCGGW	3 cut(s)	417, 1048, 2770
BsaXI	ACNNNNNCTCC	4 cut(s)	35, 65, 2039, 2069
Bse118I	RCCGGY	6 cut(s)	338, 581, 587, 758, 1001, 2257
Bse21I	CCTNAGG	1 cut(s)	1977
Bse3DI	GCAATG	3 cut(s)	1302, 1449, 2643
BseAI	TCCGGA	1 cut(s)	2770
BseBI	CCWGG	7 cut(s)	128, 332, 1581, 1944, 2073, 2485, 2632
BseGI	GGATG	6 cut(s)	33, 297, 450, 1845, 2632, 2767
BseMI	GCAATG	3 cut(s)	1302, 1449, 2643
BseMII	CTCAG	6 cut(s)	83, 479, 1091, 1311, 1991, 2200
BseRI	GAGGAG	4 cut(s)	647, 1283, 1329, 1769
BseSI	GKGCMC	4 cut(s)	84, 736, 2702, 2806
BseXI	GCAGC	6 cut(s)	102, 347, 977, 2011, 2137, 2457
BseYI	CCCAGC	1 cut(s)	603
BsgI	GTGCAG	3 cut(s)	1271, 1946, 2587
Bsh1236I	CGCG	3 cut(s)	1260, 1529, 2122
Bsh1285I	CGRYCG	3 cut(s)	422, 523, 1533
BshNI	GGYRCC	5 cut(s)	584, 590, 2046, 2736, 2803
BsiEI	CGRYCG	3 cut(s)	422, 523, 1533
BsiHKAI	GWGCWC	3 cut(s)	102, 636, 2257
BsiHKCI	CYCGRG	2 cut(s)	362, 2428
BslFI	GGGAC	2 cut(s)	1400, 2644
BsmAI	GTCTC	4 cut(s)	297, 466, 855, 1316
BsmBI	CGTCTC	2 cut(s)	297, 855
BsmFI	GGGAC	2 cut(s)	1400, 2644
BsmI	GAATGC	3 cut(s)	686, 1274, 2428
Bso31I	GGTCTC	1 cut(s)	466
BsoBI	CYCGRG	2 cut(s)	362, 2428
Bsp119I	TTCGAA	2 cut(s)	2040, 2101
Bsp120I	GGGCCC	1 cut(s)	80
Bsp1286I	GDGCHC	8 cut(s)	84, 102, 636, 736, 2257, 2304, 2702, 2806
Bsp13I	TCCGGA	1 cut(s)	2770
Bsp1407I	TGTACA	1 cut(s)	1239
Bsp143I	GATC	8 cut(s)	285, 358, 520, 972, 1137, 1347, 1898, 2583
Bsp19I	CCATGG	2 cut(s)	1789, 2322
BspCNI	CTCAG	6 cut(s)	82, 478, 1092, 1312, 1990, 2199
BspEI	TCCGGA	1 cut(s)	2770
BspFNI	CGCG	3 cut(s)	1260, 1529, 2122
BspMI	ACCTGC	1 cut(s)	448
BspPI	GGATC	3 cut(s)	353, 1132, 1342
BspQI	GCTCTTC	2 cut(s)	548, 2360
BspT104I	TTCGAA	2 cut(s)	2040, 2101
BspT107I	GGYRCC	5 cut(s)	584, 590, 2046, 2736, 2803
BspTNI	GGTCTC	1 cut(s)	466
BsrBI	CCGCTC	2 cut(s)	1711, 1998
BsrDI	GCAATG	3 cut(s)	1302, 1449, 2643
BsrFI	RCCGGY	6 cut(s)	338, 581, 587, 758, 1001, 2257
BsrGI	TGTACA	1 cut(s)	1239
BssAI	RCCGGY	6 cut(s)	338, 581, 587, 758, 1001, 2257
BssMI	GATC	8 cut(s)	285, 358, 520, 972, 1137, 1347, 1898, 2583
BssNI	GRCGYC	4 cut(s)	591, 864, 2682, 2737
BssSI	CACGAG	1 cut(s)	17
BssT1I	CCWWGG	5 cut(s)	705, 1673, 1789, 1812, 2322
Bst2BI	CACGAG	1 cut(s)	17
Bst2UI	CCWGG	7 cut(s)	128, 332, 1581, 1944, 2073, 2485, 2632
Bst4CI	ACNGT	3 cut(s)	8, 2137, 2798
Bst6I	CTCTTC	4 cut(s)	548, 670, 2168, 2360
BstACI	GRCGYC	4 cut(s)	591, 864, 2682, 2737
BstAUI	TGTACA	1 cut(s)	1239
BstBAI	YACGTR	2 cut(s)	280, 2705
BstBI	TTCGAA	2 cut(s)	2040, 2101
BstC8I	GCNNGC	9 cut(s)	340, 369, 378, 483, 1003, 1094, 1873, 2000, 2761
BstDSI	CCRYGG	3 cut(s)	1514, 1789, 2322
BstENI	CCTNNNNNAGG	1 cut(s)	1811
BstF5I	GGATG	6 cut(s)	33, 297, 450, 1845, 2632, 2767
BstFNI	CGCG	3 cut(s)	1260, 1529, 2122
BstH2I	RGCGCY	4 cut(s)	207, 594, 625, 2740
BstHHI	GCGC	7 cut(s)	206, 593, 624, 781, 1495, 2024, 2739
BstKTI	GATC	8 cut(s)	288, 361, 523, 975, 1140, 1350, 1901, 2586
BstMAI	GTCTC	4 cut(s)	297, 466, 855, 1316
BstMBI	GATC	8 cut(s)	285, 358, 520, 972, 1137, 1347, 1898, 2583
BstMCI	CGRYCG	3 cut(s)	422, 523, 1533
BstNI	CCWGG	7 cut(s)	128, 332, 1581, 1944, 2073, 2485, 2632
BstNSI	RCATGY	3 cut(s)	1487, 1969, 2011
BstSLI	GKGCMC	4 cut(s)	84, 736, 2702, 2806
BstUI	CGCG	3 cut(s)	1260, 1529, 2122
BstV1I	GCAGC	6 cut(s)	102, 347, 977, 2011, 2137, 2457
BstV2I	GAAGAC	1 cut(s)	2382
BstX2I	RGATCY	1 cut(s)	358
BstYI	RGATCY	1 cut(s)	358
Bsu36I	CCTNAGG	1 cut(s)	1977
BtgI	CCRYGG	3 cut(s)	1514, 1789, 2322
BtrI	CACGTC	3 cut(s)	165, 441, 1503
BtsCI	GGATG	6 cut(s)	33, 297, 450, 1845, 2632, 2767
BtsI	GCAGTG	3 cut(s)	636, 1958, 2455
BtsIMutI	CAGTG	7 cut(s)	636, 879, 1722, 1958, 2105, 2455, 2614
BveI	ACCTGC	1 cut(s)	448
Cac8I	GCNNGC	9 cut(s)	340, 369, 378, 483, 1003, 1094, 1873, 2000, 2761
CaiI	CAGNNNCTG	3 cut(s)	472, 1099, 1111
CfoI	GCGC	7 cut(s)	206, 593, 624, 781, 1495, 2024, 2739
Cfr10I	RCCGGY	6 cut(s)	338, 581, 587, 758, 1001, 2257
CseI	GACGC	7 cut(s)	316, 397, 872, 1129, 2080, 2128, 2671
Csp6I	GTAC	9 cut(s)	228, 240, 1165, 1240, 1255, 1543, 2005, 2614, 2842
CviQI	GTAC	9 cut(s)	228, 240, 1165, 1240, 1255, 1543, 2005, 2614, 2842
DinI	GGCGCC	2 cut(s)	592, 2738
DpnI	GATC	8 cut(s)	287, 360, 522, 974, 1139, 1349, 1900, 2585
DpnII	GATC	8 cut(s)	285, 358, 520, 972, 1137, 1347, 1898, 2583
DrdI	GACNNNNNNGTC	1 cut(s)	311
DriI	GACNNNNNGTC	1 cut(s)	61
DseDI	GACNNNNNNGTC	1 cut(s)	311
EaeI	YGGCCR	2 cut(s)	1054, 1647
Eam1104I	CTCTTC	4 cut(s)	548, 670, 2168, 2360
Eam1105I	GACNNNNNGTC	1 cut(s)	61
EarI	CTCTTC	4 cut(s)	548, 670, 2168, 2360
EciI	GGCGGA	5 cut(s)	208, 357, 763, 2383, 2576
Ecl136II	GAGCTC	2 cut(s)	100, 634
Eco130I	CCWWGG	5 cut(s)	705, 1673, 1789, 1812, 2322
Eco147I	AGGCCT	1 cut(s)	2492
Eco24I	GRGCYC	4 cut(s)	84, 102, 636, 2304
Eco31I	GGTCTC	1 cut(s)	466
Eco32I	GATATC	2 cut(s)	766, 2755
Eco47I	GGWCC	4 cut(s)	525, 1573, 2243, 2821
Eco47III	AGCGCT	1 cut(s)	623
Eco53kI	GAGCTC	2 cut(s)	100, 634
Eco57I	CTGAAG	4 cut(s)	475, 494, 903, 1133
Eco72I	CACGTG	1 cut(s)	2705
Eco81I	CCTNAGG	1 cut(s)	1977
Eco88I	CYCGRG	2 cut(s)	362, 2428
EcoICRI	GAGCTC	2 cut(s)	100, 634
EcoNI	CCTNNNNNAGG	1 cut(s)	1811
EcoO109I	RGGNCCY	2 cut(s)	501, 2821
EcoRI	GAATTC	1 cut(s)	791
EcoRII	CCWGG	7 cut(s)	126, 330, 1579, 1942, 2071, 2483, 2630
EcoRV	GATATC	2 cut(s)	766, 2755
EcoT14I	CCWWGG	5 cut(s)	705, 1673, 1789, 1812, 2322
EcoT22I	ATGCAT	1 cut(s)	686
EcoT38I	GRGCYC	4 cut(s)	84, 102, 636, 2304
EgeI	GGCGCC	2 cut(s)	592, 2738
EheI	GGCGCC	2 cut(s)	592, 2738
ErhI	CCWWGG	5 cut(s)	705, 1673, 1789, 1812, 2322
Esp3I	CGTCTC	2 cut(s)	297, 855
FaqI	GGGAC	2 cut(s)	1400, 2644
FauI	CCCGC	1 cut(s)	1991
FbaI	TGATCA	1 cut(s)	972
FblI	GTMKAC	1 cut(s)	615
FokI	GGATG	6 cut(s)	40, 284, 437, 1852, 2639, 2754
FriOI	GRGCYC	4 cut(s)	84, 102, 636, 2304
FspAI	RTGCGCAY	1 cut(s)	1494
FspBI	CTAG	3 cut(s)	986, 2217, 2399
FspI	TGCGCA	1 cut(s)	1494
GlaI	GCGC	7 cut(s)	205, 592, 623, 780, 1494, 2023, 2738
GsaI	CCCAGC	1 cut(s)	607
GsuI	CTGGAG	3 cut(s)	413, 2094, 2639
HaeII	RGCGCY	4 cut(s)	207, 594, 625, 2740
HgaI	GACGC	7 cut(s)	316, 397, 872, 1129, 2080, 2128, 2671
HhaI	GCGC	7 cut(s)	206, 593, 624, 781, 1495, 2024, 2739
Hin1I	GRCGYC	4 cut(s)	591, 864, 2682, 2737
Hin6I	GCGC	7 cut(s)	204, 591, 622, 779, 1493, 2022, 2737
HinP1I	GCGC	7 cut(s)	204, 591, 622, 779, 1493, 2022, 2737
HincII	GTYRAC	1 cut(s)	2017
HindII	GTYRAC	1 cut(s)	2017
HphI	GGTGA	7 cut(s)	5, 562, 1294, 1801, 2061, 2338, 2518
HpyCH4III	ACNGT	3 cut(s)	8, 2137, 2798
HpyCH4IV	ACGT	7 cut(s)	164, 279, 440, 1502, 1661, 2283, 2704
HpyCH4V	TGCA	9 cut(s)	684, 923, 965, 1252, 1302, 1958, 1963, 2200, 2604
HpySE526I	ACGT	7 cut(s)	164, 279, 440, 1502, 1661, 2283, 2704
Hsp92I	GRCGYC	4 cut(s)	591, 864, 2682, 2737
HspAI	GCGC	7 cut(s)	204, 591, 622, 779, 1493, 2022, 2737
KasI	GGCGCC	2 cut(s)	590, 2736
Kpn2I	TCCGGA	1 cut(s)	2770
KroI	GCCGGC	2 cut(s)	338, 1001
KroNI	GCCGGC	2 cut(s)	340, 1003
Ksp22I	TGATCA	1 cut(s)	972
Kzo9I	GATC	8 cut(s)	285, 358, 520, 972, 1137, 1347, 1898, 2583
LguI	GCTCTTC	2 cut(s)	548, 2360
Lsp1109I	GCAGC	6 cut(s)	102, 347, 977, 2011, 2137, 2457
LweI	GCATC	7 cut(s)	18, 102, 1136, 1194, 1803, 1914, 2617
MaeI	CTAG	3 cut(s)	986, 2217, 2399
MaeII	ACGT	7 cut(s)	164, 279, 440, 1502, 1661, 2283, 2704
MaeIII	GTNAC	3 cut(s)	350, 892, 2506
MalI	GATC	8 cut(s)	287, 360, 522, 974, 1139, 1349, 1900, 2585
MbiI	CCGCTC	2 cut(s)	1711, 1998
MboI	GATC	8 cut(s)	285, 358, 520, 972, 1137, 1347, 1898, 2583
MflI	RGATCY	1 cut(s)	358
MhlI	GDGCHC	8 cut(s)	84, 102, 636, 736, 2257, 2304, 2702, 2806
MlsI	TGGCCA	2 cut(s)	1056, 1649
MluCI	AATT	8 cut(s)	172, 791, 1021, 1374, 1400, 1435, 2831, 2887
MluNI	TGGCCA	2 cut(s)	1056, 1649
Mly113I	GGCGCC	2 cut(s)	591, 2737
MlyI	GAGTC	8 cut(s)	71, 302, 710, 825, 1111, 1200, 1318, 2399
MmeI	TCCRAC	8 cut(s)	78, 201, 279, 672, 834, 1438, 1450, 1456
Mox20I	TGGCCA	2 cut(s)	1056, 1649
Mph1103I	ATGCAT	1 cut(s)	686
MroI	TCCGGA	1 cut(s)	2770
MroNI	GCCGGC	2 cut(s)	338, 1001
MroXI	GAANNNNTTC	1 cut(s)	515
MscI	TGGCCA	2 cut(s)	1056, 1649
MseI	TTAA	3 cut(s)	198, 1335, 1433
MslI	CAYNNNNRTG	5 cut(s)	689, 1488, 2360, 2609, 2648
Msp20I	TGGCCA	2 cut(s)	1056, 1649
MspA1I	CMGCKG	2 cut(s)	120, 144
Mva1269I	GAATGC	3 cut(s)	686, 1274, 2428
MvaI	CCWGG	7 cut(s)	128, 332, 1581, 1944, 2073, 2485, 2632
MvnI	CGCG	3 cut(s)	1260, 1529, 2122
NaeI	GCCGGC	2 cut(s)	340, 1003
NarI	GGCGCC	2 cut(s)	591, 2737
NciI	CCSGG	6 cut(s)	79, 84, 182, 731, 992, 1082
NcoI	CCATGG	2 cut(s)	1789, 2322
NdeII	GATC	8 cut(s)	285, 358, 520, 972, 1137, 1347, 1898, 2583
NgoMIV	GCCGGC	2 cut(s)	338, 1001
NmeAIII	GCCGAG	2 cut(s)	1194, 2804
NmuCI	GTSAC	2 cut(s)	892, 2506
NsbI	TGCGCA	1 cut(s)	1494
NsiI	ATGCAT	1 cut(s)	686
NspI	RCATGY	3 cut(s)	1487, 1969, 2011
NspV	TTCGAA	2 cut(s)	2040, 2101
OliI	CACNNNNGTG	1 cut(s)	2609
PaeR7I	CTCGAG	1 cut(s)	2428
PasI	CCCWGGG	1 cut(s)	127
PceI	AGGCCT	1 cut(s)	2492
PciI	ACATGT	3 cut(s)	1483, 1965, 2007
PciSI	GCTCTTC	2 cut(s)	548, 2360
PcsI	WCGNNNNNNNCGW	1 cut(s)	360
PctI	GAATGC	3 cut(s)	686, 1274, 2428
PdiI	GCCGGC	2 cut(s)	340, 1003
PdmI	GAANNNNTTC	1 cut(s)	515
PfeI	GAWTC	8 cut(s)	824, 876, 1461, 1921, 2307, 2384, 2545, 2590
PflFI	GACNNNGTC	3 cut(s)	163, 311, 529
PflMI	CCANNNNNTGG	2 cut(s)	1818, 2165
PfoI	TCCNGGA	1 cut(s)	1942
Ple19I	CGATCG	1 cut(s)	523
PleI	GAGTC	8 cut(s)	70, 302, 709, 825, 1110, 1199, 1318, 2398
PluTI	GGCGCC	2 cut(s)	594, 2740
PmaCI	CACGTG	1 cut(s)	2705
PmlI	CACGTG	1 cut(s)	2705
PpsI	GAGTC	8 cut(s)	70, 302, 709, 825, 1110, 1199, 1318, 2398
Ppu21I	YACGTR	2 cut(s)	280, 2705
PpuMI	RGGWCCY	1 cut(s)	2821
PscI	ACATGT	3 cut(s)	1483, 1965, 2007
PsiI	TTATAA	1 cut(s)	905
Psp124BI	GAGCTC	2 cut(s)	102, 636
Psp5II	RGGWCCY	1 cut(s)	2821
Psp6I	CCWGG	7 cut(s)	126, 330, 1579, 1942, 2071, 2483, 2630
PspCI	CACGTG	1 cut(s)	2705
PspFI	CCCAGC	1 cut(s)	603
PspGI	CCWGG	7 cut(s)	126, 330, 1579, 1942, 2071, 2483, 2630
PspOMI	GGGCCC	1 cut(s)	80
PspPPI	RGGWCCY	1 cut(s)	2821
PspXI	VCTCGAGB	1 cut(s)	2428
PstNI	CAGNNNCTG	3 cut(s)	472, 1099, 1111
PsuI	RGATCY	1 cut(s)	358
PsyI	GACNNNGTC	3 cut(s)	163, 311, 529
PvuI	CGATCG	1 cut(s)	523
RsaI	GTAC	9 cut(s)	229, 241, 1166, 1241, 1256, 1544, 2006, 2615, 2843
RsaNI	GTAC	9 cut(s)	228, 240, 1165, 1240, 1255, 1543, 2005, 2614, 2842
RseI	CAYNNNNRTG	5 cut(s)	689, 1488, 2360, 2609, 2648
SacI	GAGCTC	2 cut(s)	102, 636
SapI	GCTCTTC	2 cut(s)	548, 2360
SaqAI	TTAA	3 cut(s)	198, 1335, 1433
Sau3AI	GATC	8 cut(s)	285, 358, 520, 972, 1137, 1347, 1898, 2583
ScaI	AGTACT	2 cut(s)	241, 1166
SchI	GAGTC	8 cut(s)	71, 302, 710, 825, 1111, 1200, 1318, 2399
SduI	GDGCHC	8 cut(s)	84, 102, 636, 736, 2257, 2304, 2702, 2806
SfaNI	GCATC	7 cut(s)	18, 102, 1136, 1194, 1803, 1914, 2617
SfoI	GGCGCC	2 cut(s)	592, 2738
Sfr274I	CTCGAG	1 cut(s)	2428
SfuI	TTCGAA	2 cut(s)	2040, 2101
SgrAI	CRCCGGYG	1 cut(s)	587
SinI	GGWCC	4 cut(s)	525, 1573, 2243, 2821
SlaI	CTCGAG	1 cut(s)	2428
SmiMI	CAYNNNNRTG	5 cut(s)	689, 1488, 2360, 2609, 2648
SmlI	CTYRAG	6 cut(s)	560, 1328, 1712, 1925, 2428, 2825
SmoI	CTYRAG	6 cut(s)	560, 1328, 1712, 1925, 2428, 2825
Sse9I	AATT	8 cut(s)	172, 791, 1021, 1374, 1400, 1435, 2831, 2887
SseBI	AGGCCT	1 cut(s)	2492
SspDI	GGCGCC	2 cut(s)	590, 2736
SspMI	CTAG	3 cut(s)	986, 2217, 2399
SstI	GAGCTC	2 cut(s)	102, 636
StuI	AGGCCT	1 cut(s)	2492
StyI	CCWWGG	5 cut(s)	705, 1673, 1789, 1812, 2322
TaaI	ACNGT	3 cut(s)	8, 2137, 2798
TaiI	ACGT	7 cut(s)	167, 282, 443, 1505, 1664, 2286, 2707
TaqII	GACCGA	1 cut(s)	1547
TasI	AATT	8 cut(s)	172, 791, 1021, 1374, 1400, 1435, 2831, 2887
TatI	WGTACW	5 cut(s)	239, 1164, 1239, 2004, 2841
TauI	GCSGC	9 cut(s)	90, 123, 147, 481, 611, 1711, 2125, 2409, 2766
TfiI	GAWTC	8 cut(s)	824, 876, 1461, 1921, 2307, 2384, 2545, 2590
Tru1I	TTAA	3 cut(s)	198, 1335, 1433
Tru9I	TTAA	3 cut(s)	198, 1335, 1433
TscAI	CASTG	7 cut(s)	643, 886, 1729, 1965, 2112, 2455, 2614
TseFI	GTSAC	2 cut(s)	892, 2506
TseI	GCWGC	6 cut(s)	90, 335, 965, 2024, 2125, 2445
Tsp45I	GTSAC	2 cut(s)	892, 2506
TspDTI	ATGAA	4 cut(s)	17, 556, 707, 2378
TspGWI	ACGGA	4 cut(s)	245, 301, 1531, 2265
TspRI	CASTG	7 cut(s)	643, 886, 1729, 1965, 2112, 2455, 2614
Tth111I	GACNNNGTC	3 cut(s)	163, 311, 529
Van91I	CCANNNNNTGG	2 cut(s)	1818, 2165
VpaK11BI	GGWCC	4 cut(s)	525, 1573, 2243, 2821
XagI	CCTNNNNNAGG	1 cut(s)	1811
XapI	RAATTY	4 cut(s)	791, 1021, 1435, 2887
XceI	RCATGY	3 cut(s)	1487, 1969, 2011
XcmI	CCANNNNNNNNNTGG	2 cut(s)	388, 1049
XhoI	CTCGAG	1 cut(s)	2428
XmiI	GTMKAC	1 cut(s)	615
XmnI	GAANNNNTTC	1 cut(s)	515
XspI	CTAG	3 cut(s)	986, 2217, 2399
ZrmI	AGTACT	2 cut(s)	241, 1166
Zsp2I	ATGCAT	1 cut(s)	686

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.