pycom16g11570

female pronucleus assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0016205
Physical Location & Seq
Reverse (-)
8058157 .. 8059990
1834 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
ATGGCTGTGAATCCTCAACTGTTCCCCAATGGCATGCCCGTTCCTTTCGTGAACGAGATGTTCGTTTTGGCCAGAGACGGCGTCGAGTTCGAGGTCGATAAGATTCCCGGATATGATGGTGGTCGTCTGAAAGCAAAAGGAACAATCTACTTATCAAATATACGGATGGTCTTTGTTTCCAACAAGCCTGTTGGGAGCTTTTTTGCTTTTGATATGCCCCTGCTTTATGTCCATGGTGAAAAGTTTAATCAACCAATTTTCCACTGCAACAACATTGCTGGTCAGGTGGAGCCAGTGGTTCCAGAAGGTCAGAATGGGGCGCTTTATTCCACTCACTCATTCAAGATTTTGTTCAAAGAAGGGGGCTGTGGGACGTTCGTTCCACTCTTTTTCAACTTGATCTCTGCAGTGAGACAATATAACCAGTATCAACACCCCAACGCTGGGTCTCAGCCTCTTGTGGATCCTTTGCAAGCAGCACAAACTCCTGTTGATGAGATGATGAGACATGCGTATGTCGATCCTAATGATCCAACGAGGATCTTCTTGCAGCAGCCCACACCAGAGGCTCAGCTGAGGCGGCGCACATACCAGTCACAGCCCGCCGAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

22.95

Weight (kDa)

6.29

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11680
fragaria_vesca FvH4_4g21930
malus_domestica MD13G1135700.v1.1 MD16G1131300.v1.1
prunus_persica Prupe.1G209600_v2.0.a1
pyrus_communis pycom16g11570
rosa_chinensis RchiOBHm_Chr4g0428141
rosa_laevigata RLG00000007176
rosa_multiflora Rmu_sc0004940.1_g000010 Rmu_sc0009599.1_g000010
rosa_roxburghii Rroxscaffold_5G00369870
rosa_rugosa Rorug04G0222900
rosa_samantha Rh4BG285700 Rh4CG301000
rosa_wichuraiana Rw4G024330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 580, 603
AclWI GGATC 5 cut(s) 458, 471, 515, 524, 548
AcoI YGGCCR 1 cut(s) 69
AcyI GRCGYC 1 cut(s) 81
AfiI CCNNNNNNNGG 2 cut(s) 443, 444
AgsI TTSAA 3 cut(s) 343, 355, 394
AluBI AGCT 2 cut(s) 198, 574
AluI AGCT 2 cut(s) 198, 574
Alw26I GTCTC 4 cut(s) 69, 406, 453, 499
AlwI GGATC 5 cut(s) 458, 471, 515, 524, 548
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 3 cut(s) 476, 550, 553
AspLEI GCGC 2 cut(s) 322, 585
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 248
BalI TGGCCA 1 cut(s) 71
BamHI GGATCC 1 cut(s) 463
BbvCI CCTCAGC 1 cut(s) 575
BbvI GCAGC 3 cut(s) 488, 562, 565
BccI CCATC 2 cut(s) 110, 160
BceAI ACGGC 1 cut(s) 94
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 4 cut(s) 69, 406, 453, 499
BfmI CTRYAG 1 cut(s) 405
BfoI RGCGCY 1 cut(s) 323
BisI GCNGC 4 cut(s) 477, 551, 554, 581
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 4 cut(s) 478, 552, 555, 582
Bme1390I CCNGG 1 cut(s) 108
BmiI GGNNCC 3 cut(s) 291, 300, 465
BmrFI CCNGG 1 cut(s) 108
Bpu10I CCTNAGC 1 cut(s) 575
Bpu1102I GCTNAGC 1 cut(s) 570
BpuMI CCSGG 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 453
BsaJI CCNNGG 1 cut(s) 232
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 444
Bse1I ACTGG 3 cut(s) 293, 424, 592
Bse3DI GCAATG 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 232
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 2 cut(s) 443, 444
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 3 cut(s) 464, 566, 584
BseNI ACTGG 3 cut(s) 293, 424, 592
BseXI GCAGC 3 cut(s) 488, 562, 565
BseYI CCCAGC 1 cut(s) 443
BshFI GGCC 1 cut(s) 71
BsiSI CCGG 1 cut(s) 108
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 2 cut(s) 443, 444
BsmAI GTCTC 4 cut(s) 69, 406, 453, 499
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 385
BsnI GGCC 1 cut(s) 71
Bso31I GGTCTC 1 cut(s) 453
Bsp143I GATC 5 cut(s) 399, 463, 520, 529, 540
Bsp1720I GCTNAGC 1 cut(s) 570
Bsp19I CCATGG 1 cut(s) 232
BspACI CCGC 2 cut(s) 580, 603
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 3 cut(s) 463, 567, 583
BspLI GGNNCC 3 cut(s) 291, 300, 465
BspMAI CTGCAG 1 cut(s) 409
BspPI GGATC 5 cut(s) 458, 471, 515, 524, 548
BspTNI GGTCTC 1 cut(s) 453
BsrDI GCAATG 1 cut(s) 273
BsrI ACTGG 3 cut(s) 293, 424, 592
BssECI CCNNGG 1 cut(s) 232
BssMI GATC 5 cut(s) 399, 463, 520, 529, 540
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 232
Bst4CI ACNGT 1 cut(s) 21
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 3 cut(s) 35, 474, 603
BstDEI CTNAG 3 cut(s) 450, 570, 575
BstDSI CCRYGG 1 cut(s) 232
BstF5I GGATG 1 cut(s) 171
BstH2I RGCGCY 1 cut(s) 323
BstHHI GCGC 2 cut(s) 322, 585
BstKTI GATC 5 cut(s) 402, 466, 523, 532, 543
BstMAI GTCTC 4 cut(s) 69, 406, 453, 499
BstMBI GATC 5 cut(s) 399, 463, 520, 529, 540
BstMWI GCNNNNNNNGC 1 cut(s) 580
BstNSI RCATGY 2 cut(s) 37, 512
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 405
BstV1I GCAGC 3 cut(s) 488, 562, 565
BstX2I RGATCY 2 cut(s) 463, 540
BstYI RGATCY 2 cut(s) 463, 540
BsuRI GGCC 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 232
BtsCI GGATG 1 cut(s) 171
BtsI GCAGTG 2 cut(s) 262, 414
BtsIMutI CAGTG 3 cut(s) 262, 300, 414
Cac8I GCNNGC 3 cut(s) 35, 474, 603
CfoI GCGC 2 cut(s) 322, 585
CseI GACGC 1 cut(s) 70
CviAII CATG 3 cut(s) 34, 233, 509
DdeI CTNAG 3 cut(s) 450, 570, 575
DpnI GATC 5 cut(s) 401, 465, 522, 531, 542
DpnII GATC 5 cut(s) 399, 463, 520, 529, 540
EaeI YGGCCR 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 232
Eco31I GGTCTC 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 232
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 3 cut(s) 37, 236, 512
FaqI GGGAC 1 cut(s) 385
FatI CATG 3 cut(s) 33, 232, 508
FauI CCCGC 1 cut(s) 610
Fnu4HI GCNGC 4 cut(s) 477, 551, 554, 581
FokI GGATG 1 cut(s) 178
Fsp4HI GCNGC 4 cut(s) 477, 551, 554, 581
GlaI GCGC 2 cut(s) 321, 584
GluI GCNGC 4 cut(s) 477, 551, 554, 581
GsaI CCCAGC 1 cut(s) 447
HaeII RGCGCY 1 cut(s) 323
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 108
HgaI GACGC 1 cut(s) 70
HhaI GCGC 2 cut(s) 322, 585
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 3 cut(s) 37, 236, 512
Hin6I GCGC 2 cut(s) 320, 583
HinP1I GCGC 2 cut(s) 320, 583
HinfI GANTC 2 cut(s) 10, 103
HpaII CCGG 1 cut(s) 108
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 2 cut(s) 129, 312
Hpy188III TCNNGA 3 cut(s) 49, 302, 343
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 2 cut(s) 299, 353
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 4 cut(s) 267, 407, 472, 550
HpyF10VI GCNNNNNNNGC 1 cut(s) 580
HpyF3I CTNAG 3 cut(s) 450, 570, 575
HpySE526I ACGT 1 cut(s) 374
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 3 cut(s) 37, 236, 512
HspAI GCGC 2 cut(s) 320, 583
Kzo9I GATC 5 cut(s) 399, 463, 520, 529, 540
LmnI GCTCC 2 cut(s) 195, 289
Lsp1109I GCAGC 3 cut(s) 488, 562, 565
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 1 cut(s) 594
MalI GATC 5 cut(s) 401, 465, 522, 531, 542
MboI GATC 5 cut(s) 399, 463, 520, 529, 540
MboII GAAGA 1 cut(s) 535
MflI RGATCY 2 cut(s) 463, 540
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 1 cut(s) 255
MluNI TGGCCA 1 cut(s) 71
MmeI TCCRAC 2 cut(s) 204, 557
MnlI CCTC 6 cut(s) 24, 85, 465, 531, 559, 570
Mox20I TGGCCA 1 cut(s) 71
MscI TGGCCA 1 cut(s) 71
MseI TTAA 2 cut(s) 246, 613
MslI CAYNNNNRTG 1 cut(s) 513
Msp20I TGGCCA 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 574
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 580
NciI CCSGG 1 cut(s) 108
NcoI CCATGG 1 cut(s) 232
NdeII GATC 5 cut(s) 399, 463, 520, 529, 540
NlaIII CATG 3 cut(s) 37, 236, 512
NlaIV GGNNCC 3 cut(s) 291, 300, 465
NmuCI GTSAC 1 cut(s) 594
NspI RCATGY 2 cut(s) 37, 512
PaeI GCATGC 1 cut(s) 37
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PfeI GAWTC 2 cut(s) 10, 103
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 106
PkrI GCNGC 4 cut(s) 478, 552, 555, 582
PspFI CCCAGC 1 cut(s) 443
PspN4I GGNNCC 3 cut(s) 291, 300, 465
PstI CTGCAG 1 cut(s) 409
PsuI RGATCY 2 cut(s) 463, 540
PsyI GACNNNGTC 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 574
RseI CAYNNNNRTG 1 cut(s) 513
SaqAI TTAA 2 cut(s) 246, 613
SatI GCNGC 4 cut(s) 477, 551, 554, 581
Sau3AI GATC 5 cut(s) 399, 463, 520, 529, 540
ScrFI CCNGG 1 cut(s) 108
SetI ASST 6 cut(s) 96, 200, 288, 310, 377, 576
SfcI CTRYAG 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 513
SphI GCATGC 1 cut(s) 37
Sse9I AATT 1 cut(s) 255
SsiI CCGC 2 cut(s) 580, 603
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 232
TaaI ACNGT 1 cut(s) 21
TaiI ACGT 1 cut(s) 377
TaqI TCGA 4 cut(s) 84, 90, 96, 519
TasI AATT 1 cut(s) 255
TauI GCSGC 1 cut(s) 583
TfiI GAWTC 2 cut(s) 10, 103
Tru1I TTAA 2 cut(s) 246, 613
Tru9I TTAA 2 cut(s) 246, 613
TscAI CASTG 3 cut(s) 269, 300, 414
TseFI GTSAC 1 cut(s) 594
TseI GCWGC 3 cut(s) 476, 550, 553
Tsp45I GTSAC 1 cut(s) 594
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 3 cut(s) 269, 300, 414
Tth111I GACNNNGTC 1 cut(s) 80
XceI RCATGY 2 cut(s) 37, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.