pycom16g13720

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0017337
Physical Location & Seq
Reverse (-)
9797663 .. 9800477
2815 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1182 bp
ATGCACTCTTCATCAAGGGTTCACTGTGATAAGCATGGATTTCCACCCTTCTCACCACACATTACTTTTGGCTGTAGTAATGGCGAAATTGCACTATGGGAAGTTGGGATGCGAGAGAGGCTAGTGTCAAAGCCATTCAAAGTATGGAATATGACTACTTATTCTAATGCATTTCAGGTTGCTATGGTCAAAGATACAGCGATATCTGTAAGCCGTGTATCATGGAATCAAGATGGAAGCTTAATCGGTGTTGCATTTACCAAACACTTGGTTCATTTGTACGCTTATCAAGGACCTACTGATCTACGGCAACATTTGGAGATTGACGCTCACAGTGGTAGTGTGAATGACTTAGCCTTTTCTCTTCCAAACAAGCAATTATGTCTTATAACGTGTGGGGAGGACAAGCTGATAAAGGTTTGGGATTTGGCTGGAACAAAACTATTCTGCTTTGAAGGTCATGAAGCACCTGTTTACTCGATTTGCCCCCACCAGAAGGAGAATATTCAGTTCATATTTTCAACTGCTGTTGATGGGAAAATAAAAGCTTGGCTTTATGACAATGTGGGCTCCAGGGTTGACTATGATGCTCCTGGGCACTGTTGCACCACAATGCTATACAGTGATGACGGAAATAGATTATTCTCTTGTGGGACTAGTAAAGATGGTGATTCTTTCCTCGTTGAATGGAATGAAAGTGAAGGGGCAATAAAGAGGACATATAACGGATTCCGAAAAAAATCTTCTAGGATAGTGCAGTTTGATACAACAAAAAATCACTTTTTGGCTGTTGGTGAAGATAACCAGATAAAGTTTTGGGATATGGATAGTACAAATGTCCTTACTACTACAGATGCTGAGGGTGGACTTCTGACTCTTCCCCGTTTGAGATTCAATAAGGAAGGAAATTTGCTTGCTGCTACCACGGCTGACAACGGAATCAAAGTTCTTGCTAATGCTGAGGGTTTCAGATCCTTAAGAGCAATTGAAACTCGATCTTATGAAGTATCCAGAGCACCTATTGAGATGAAGGTATCTGGTTCTTCTATGGTTCCGAACATCAACCCAACCATTAATAAAGTGGAACGTATGGATACAAGCTCTCCTGCCAGACCGACTCATATTCTTAATGGAGGCCGATTCTATGGCAAGAAGCCTGGAAAAGAGAAGAAGTTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

394

Amino Acids

43.91

Weight (kDa)

8.46

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 24 - 221 3.5e-07 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 46 - 157 6.7e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 47 - 184 2.7e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 47 - 147 2.7e-08 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 67 - 281 1.6e-18 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 69 - 329 1.8e-22 THOC3 beta-propeller domain
WDR55 PF24796 70 - 184 6.8e-06 WDR55
WD40_WDHD1_1st PF24817 115 - 187 7.9e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 123 - 256 5e-06 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 155 - 317 2.9e-08 WDR55
WD40_Prp19 PF24814 166 - 316 8.9e-07 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 178 - 316 2.9e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 201 - 338 4.7e-07 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 389
AclWI GGATC 1 cut(s) 966
AcsI RAATTY 1 cut(s) 907
AfaI GTAC 2 cut(s) 281, 832
AfiI CCNNNNNNNGG 1 cut(s) 496
AflII CTTAAG 1 cut(s) 976
AflIII ACRYGT 1 cut(s) 392
AgsI TTSAA 6 cut(s) 139, 455, 522, 686, 895, 989
AhlI ACTAGT 1 cut(s) 656
AjnI CCWGG 3 cut(s) 572, 592, 1156
AloI GAACNNNNNNTCC 2 cut(s) 930, 962
AluBI AGCT 4 cut(s) 240, 409, 548, 1101
AluI AGCT 4 cut(s) 240, 409, 548, 1101
Alw21I GWGCWC 1 cut(s) 1018
AlwI GGATC 1 cut(s) 966
AlwNI CAGNNNCTG 1 cut(s) 857
AoxI GGCC 1 cut(s) 1135
ApeKI GCWGC 1 cut(s) 917
ApoI RAATTY 1 cut(s) 907
AseI ATTAAT 1 cut(s) 1074
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 3 cut(s) 45, 680, 806
AvaII GGWCC 1 cut(s) 293
BaeGI GKGCMC 1 cut(s) 600
BanII GRGCYC 1 cut(s) 572
Bbv12I GWGCWC 1 cut(s) 1018
BbvCI CCTCAGC 2 cut(s) 858, 960
BbvI GCAGC 1 cut(s) 904
BccI CCATC 3 cut(s) 227, 527, 659
BceAI ACGGC 3 cut(s) 198, 323, 942
BciT130I CCWGG 3 cut(s) 574, 594, 1158
BciVI GTATCC 2 cut(s) 1018, 1087
BcuI ACTAGT 1 cut(s) 656
BfaI CTAG 3 cut(s) 122, 657, 747
BfmI CTRYAG 2 cut(s) 73, 849
BfrI CTTAAG 1 cut(s) 976
BfuI GTATCC 2 cut(s) 1018, 1087
BisI GCNGC 1 cut(s) 918
BlsI GCNGC 1 cut(s) 919
Bme1390I CCNGG 3 cut(s) 574, 594, 1158
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 571, 1053
BmrFI CCNGG 3 cut(s) 574, 594, 1158
BmsI GCATC 3 cut(s) 99, 577, 844
BpmI CTGGAG 1 cut(s) 556
Bpu10I CCTNAGC 2 cut(s) 858, 960
BsaJI CCNNGG 3 cut(s) 573, 593, 924
BsaXI ACNNNNNCTCC 2 cut(s) 1087, 1117
Bsc4I CCNNNNNNNGG 1 cut(s) 496
BseBI CCWGG 3 cut(s) 574, 594, 1158
BseDI CCNNGG 3 cut(s) 573, 593, 924
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 496
BseMII CTCAG 2 cut(s) 849, 951
BseSI GKGCMC 1 cut(s) 600
BseXI GCAGC 1 cut(s) 904
BsgI GTGCAG 1 cut(s) 776
BshFI GGCC 1 cut(s) 1137
BsiHKAI GWGCWC 1 cut(s) 1018
BslFI GGGAC 1 cut(s) 667
BslI CCNNNNNNNGG 1 cut(s) 496
BsmFI GGGAC 1 cut(s) 667
BsnI GGCC 1 cut(s) 1137
Bsp1286I GDGCHC 3 cut(s) 572, 600, 1018
Bsp143I GATC 3 cut(s) 301, 971, 995
BspANI GGCC 1 cut(s) 1137
BspCNI CTCAG 2 cut(s) 850, 952
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 2 cut(s) 571, 1053
BspPI GGATC 1 cut(s) 966
BspTI CTTAAG 1 cut(s) 976
BssECI CCNNGG 3 cut(s) 573, 593, 924
BssMI GATC 3 cut(s) 301, 971, 995
Bst2UI CCWGG 3 cut(s) 574, 594, 1158
Bst4CI ACNGT 4 cut(s) 26, 335, 602, 623
Bst6I CTCTTC 3 cut(s) 13, 369, 882
BstAFI CTTAAG 1 cut(s) 976
BstC8I GCNNGC 1 cut(s) 915
BstDEI CTNAG 3 cut(s) 352, 858, 960
BstDSI CCRYGG 1 cut(s) 924
BstF5I GGATG 1 cut(s) 114
BstKTI GATC 3 cut(s) 304, 974, 998
BstMBI GATC 3 cut(s) 301, 971, 995
BstMWI GCNNNNNNNGC 2 cut(s) 118, 926
BstNI CCWGG 3 cut(s) 574, 594, 1158
BstSCI CCNGG 3 cut(s) 572, 592, 1156
BstSFI CTRYAG 2 cut(s) 73, 849
BstSLI GKGCMC 1 cut(s) 600
BstV1I GCAGC 1 cut(s) 904
BstX2I RGATCY 1 cut(s) 971
BstXI CCANNNNNNTGG 1 cut(s) 268
BstYI RGATCY 1 cut(s) 971
BsuI GTATCC 2 cut(s) 1018, 1087
BsuRI GGCC 1 cut(s) 1137
BtgI CCRYGG 1 cut(s) 924
BtsCI GGATG 1 cut(s) 114
BtsIMutI CAGTG 4 cut(s) 22, 340, 598, 628
Cac8I GCNNGC 1 cut(s) 915
CaiI CAGNNNCTG 1 cut(s) 857
CciI TCATGA 1 cut(s) 460
Cfr13I GGNCC 1 cut(s) 293
CseI GACGC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 280, 831
CviAII CATG 3 cut(s) 35, 222, 461
CviQI GTAC 2 cut(s) 280, 831
DdeI CTNAG 3 cut(s) 352, 858, 960
DpnI GATC 3 cut(s) 303, 973, 997
DpnII GATC 3 cut(s) 301, 971, 995
Eam1104I CTCTTC 3 cut(s) 13, 369, 882
EarI CTCTTC 3 cut(s) 13, 369, 882
Eco24I GRGCYC 1 cut(s) 572
Eco32I GATATC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 3 cut(s) 572, 592, 1156
EcoRV GATATC 1 cut(s) 204
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 572
FaeI CATG 3 cut(s) 38, 225, 464
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 1 cut(s) 667
FatI CATG 3 cut(s) 34, 221, 460
Fnu4HI GCNGC 1 cut(s) 918
FokI GGATG 1 cut(s) 121
FriOI GRGCYC 1 cut(s) 572
Fsp4HI GCNGC 1 cut(s) 918
FspBI CTAG 3 cut(s) 122, 657, 747
GluI GCNGC 1 cut(s) 918
GsuI CTGGAG 1 cut(s) 556
HaeIII GGCC 1 cut(s) 1137
HgaI GACGC 1 cut(s) 335
Hin1II CATG 3 cut(s) 38, 225, 464
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HindIII AAGCTT 2 cut(s) 238, 546
HinfI GANTC 8 cut(s) 226, 671, 729, 874, 891, 939, 1117, 1140
HphI GGTGA 3 cut(s) 45, 680, 806
Hpy166II GTNNAC 4 cut(s) 22, 475, 580, 866
Hpy188I TCNGA 4 cut(s) 734, 873, 971, 1056
Hpy188III TCNNGA 3 cut(s) 230, 461, 1011
Hpy8I GTNNAC 4 cut(s) 22, 475, 580, 866
HpyAV CCTTC 6 cut(s) 58, 449, 490, 695, 896, 1024
HpyCH4III ACNGT 4 cut(s) 26, 335, 602, 623
HpyCH4IV ACGT 2 cut(s) 392, 1087
HpyCH4V TGCA 6 cut(s) 4, 92, 170, 254, 606, 757
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 926
HpyF3I CTNAG 3 cut(s) 352, 858, 960
HpySE526I ACGT 2 cut(s) 392, 1087
Hsp92II CATG 3 cut(s) 38, 225, 464
Kzo9I GATC 3 cut(s) 301, 971, 995
LmnI GCTCC 2 cut(s) 575, 595
Lsp1109I GCAGC 1 cut(s) 904
LweI GCATC 3 cut(s) 99, 577, 844
MaeI CTAG 3 cut(s) 122, 657, 747
MaeII ACGT 2 cut(s) 392, 1087
MalI GATC 3 cut(s) 303, 973, 997
MboI GATC 3 cut(s) 301, 971, 995
MboII GAAGA 6 cut(s) 356, 735, 809, 869, 1035, 1180
MfeI CAATTG 1 cut(s) 984
MflI RGATCY 1 cut(s) 971
MhlI GDGCHC 3 cut(s) 572, 600, 1018
MluCI AATT 4 cut(s) 87, 377, 907, 984
MlyI GAGTC 2 cut(s) 868, 1111
MnlI CCTC 7 cut(s) 111, 394, 689, 708, 853, 955, 1127
Mph1103I ATGCAT 1 cut(s) 172
MseI TTAA 4 cut(s) 242, 977, 1074, 1128
MslI CAYNNNNRTG 1 cut(s) 611
MspCI CTTAAG 1 cut(s) 976
MspR9I CCNGG 3 cut(s) 574, 594, 1158
MunI CAATTG 1 cut(s) 984
MvaI CCWGG 3 cut(s) 574, 594, 1158
MwoI GCNNNNNNNGC 2 cut(s) 118, 926
NdeII GATC 3 cut(s) 301, 971, 995
NlaIII CATG 3 cut(s) 38, 225, 464
NlaIV GGNNCC 2 cut(s) 571, 1053
NsiI ATGCAT 1 cut(s) 172
PagI TCATGA 1 cut(s) 460
PfeI GAWTC 6 cut(s) 226, 671, 729, 891, 939, 1140
PkrI GCNGC 1 cut(s) 919
PleI GAGTC 2 cut(s) 868, 1111
PpsI GAGTC 2 cut(s) 868, 1111
PpuMI RGGWCCY 1 cut(s) 293
PshBI ATTAAT 1 cut(s) 1074
PsiI TTATAA 1 cut(s) 389
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 3 cut(s) 572, 592, 1156
PspGI CCWGG 3 cut(s) 572, 592, 1156
PspN4I GGNNCC 2 cut(s) 571, 1053
PspPI GGNCC 1 cut(s) 293
PspPPI RGGWCCY 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 1 cut(s) 971
RsaI GTAC 2 cut(s) 281, 832
RsaNI GTAC 2 cut(s) 280, 831
RseI CAYNNNNRTG 1 cut(s) 611
SaqAI TTAA 4 cut(s) 242, 977, 1074, 1128
SatI GCNGC 1 cut(s) 918
Sau3AI GATC 3 cut(s) 301, 971, 995
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 2 cut(s) 868, 1111
ScrFI CCNGG 3 cut(s) 574, 594, 1158
SduI GDGCHC 3 cut(s) 572, 600, 1018
SfaNI GCATC 3 cut(s) 99, 577, 844
SfcI CTRYAG 2 cut(s) 73, 849
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 611
SmlI CTYRAG 1 cut(s) 976
SmoI CTYRAG 1 cut(s) 976
SpeI ACTAGT 1 cut(s) 656
Sse9I AATT 4 cut(s) 87, 377, 907, 984
SspI AATATT 1 cut(s) 505
SspMI CTAG 3 cut(s) 122, 657, 747
StyD4I CCNGG 3 cut(s) 572, 592, 1156
TaaI ACNGT 4 cut(s) 26, 335, 602, 623
TaiI ACGT 2 cut(s) 395, 1090
TaqI TCGA 2 cut(s) 479, 994
TaqII GACCGA 1 cut(s) 1129
TasI AATT 4 cut(s) 87, 377, 907, 984
TatI WGTACW 1 cut(s) 830
TfiI GAWTC 6 cut(s) 226, 671, 729, 891, 939, 1140
Tru1I TTAA 4 cut(s) 242, 977, 1074, 1128
Tru9I TTAA 4 cut(s) 242, 977, 1074, 1128
TscAI CASTG 4 cut(s) 29, 340, 605, 628
TseI GCWGC 1 cut(s) 917
TspDTI ATGAA 6 cut(s) 263, 477, 502, 708, 1017, 1043
TspGWI ACGGA 3 cut(s) 645, 741, 951
TspRI CASTG 4 cut(s) 29, 340, 605, 628
Vha464I CTTAAG 1 cut(s) 976
VpaK11BI GGWCC 1 cut(s) 293
VspI ATTAAT 1 cut(s) 1074
XapI RAATTY 1 cut(s) 907
XcmI CCANNNNNNNNNTGG 2 cut(s) 141, 1078
XspI CTAG 3 cut(s) 122, 657, 747
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.