pycom16g17580

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007045
Physical Location & Seq
Reverse (-)
13810616 .. 13818006
7391 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1605 bp
ATGGATCACCATGATGGTGGGTATGGCAAGGTCAACCTCACAGTGCACATCTTGGCTGCCTCCTTTGTTGGCCTTCTGGTTGCTGCTGCTGCCACGAGCATTTATCGCCGGAAAAAGTCGGCTAAGGCGTTGAGAGCCCCAAACAACATCACTCCACGAATAGAGAGGACGGAATCCGGCCGCGTTAGGAATATCGAAAGATTCTCCCATTATGTTGCTAGGCAACTGGGATTTTTGGATGCAAATGAGTGCCCCCAGCTATGCAAGTTAGCTTATGATTACTTGAGAAAAACGAAAGGGTGTGAAGAAAACATCTACGAATATTTCGCTTCAGAAGCAGATCAAGATTCTCTCTACGTAAATTTGATTAACGAATTTGATAGATGCATCCTCAGTTATTTTGCATTTCATTGGAGCCAAGCTACTCTAATGATTAGTCAGGTTTTGAATGCAGAGACTAAGAAGATGACGAAGCTCAAAGACATGGTATTGGCAGCTACCAGGAAGCAGAGATTTGAGAGGATCAGCAAGGAGCTAAAGATGACAAGGGTGTTCTCTACTTTGGTAGATGAAATGAAAGCCATTGGAAGCACTGCAATAAAGGGAGAACCACAATGTACGGACGTGATGAAGCCGGTGGCCCATAGTGAGAGGAGTCCCGTGCTTCTTCTTATAGGTGGTGGAATGGGCGGTGGCAAAAGCACTGTCCTCAAAGATATTCTCAAAGAATCATTCTGGTCAGGAGCAGCAGCAAATGCTGTGGTAGTAGAGGCTGATGCTTTCAAAGAGACGGACGTCCTTTACAGAGCCCTTAGCTCTAGAGGTCACCACAGTGACATGCTCCAAACCGCTGAGCTAGTGCACCAATCATCCACTGATGCTGCATCATCGCTTCTAGTGACTGCACTCAATGAAGGAAGGGATGTAATCATGGACAGCACTCTATCATGGCTGCCCTTTGTTGAGCAGACAATAGACATGGCCCGGGATGTTCACAACTGCCGCTACCGCATGGGGGTCGGATACAAGGTGGAGGAGGATGGCACCATTACCGAAAACTATTGGGAACGAGTGGATGAAGAAGAGGATCATCAGGACCAGCAGAAGATAGATAATGGGGAGGAACCCAGGAGAAAACCCTACAGAATAGAGTTGGTTGGAGTGCTTTGTGATGCTTATTTAGCTGTTGTTAGAGGCATAAGAAGAGCTATCATGGTCGGGAGGGCAGTGAGGGTCAGTTCACAGTTGAATTCCCACAAGAGATTTGCTAGTTCATTTTATAGATATTGCCAGCTTGTTGACAATGCCAGGCTCTACTGCACCAATGCTACGGCTGGTCCTCCAAAGCGAATAGGATGGAAGGATGGAGACAACAAGTTGCTAGTCGATCAGGAAGCGATCAAGTGCCTGACAAACTTGAGCAATTTGAACTCCAAGGCAGAATCCATATACGAGCTTTACATGCAGAACCCAAGCCTTATTCTCGAGCCTGGTTCTCTCTGGAAAGATATTGTTTTGTGCTCCCCAAGGCCCAGCCTTCAGCTTAAGCTAAGGGCTATCATTAAAAAAATTGAAATTTCATACACTGCTAACAAATTGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

535

Amino Acids

60.15

Weight (kDa)

8.58

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 212 - 329 3.6e-13 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 798
AccB1I GGYRCC 1 cut(s) 1043
AccII CGCG 1 cut(s) 183
AciI CCGC 5 cut(s) 181, 690, 849, 1003, 1009
AclWI GGATC 3 cut(s) 12, 530, 1095
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 4 cut(s) 361, 374, 1249, 1575
AcuI CTGAAG 2 cut(s) 315, 1523
AcyI GRCGYC 1 cut(s) 795
AfaI GTAC 1 cut(s) 619
AfiI CCNNNNNNNGG 1 cut(s) 1015
AflII CTTAAG 1 cut(s) 1544
AgsI TTSAA 5 cut(s) 448, 784, 1249, 1429, 1574
AjiI CACGTC 1 cut(s) 625
AjnI CCWGG 4 cut(s) 500, 1127, 1307, 1489
AleI CACNNNNGTG 1 cut(s) 831
Alw21I GWGCWC 3 cut(s) 48, 864, 1523
Alw26I GTCTC 3 cut(s) 449, 782, 1362
Alw44I GTGCAC 2 cut(s) 44, 860
AlwI GGATC 3 cut(s) 12, 530, 1095
Ama87I CYCGRG 2 cut(s) 984, 1484
AoxI GGCC 5 cut(s) 70, 178, 639, 981, 1529
ApaLI GTGCAC 2 cut(s) 44, 860
ApeKI GCWGC 9 cut(s) 56, 83, 86, 89, 494, 746, 749, 881, 952
ApoI RAATTY 4 cut(s) 361, 374, 1249, 1575
ArsI GACNNNNNNTTYG 2 cut(s) 690, 722
AspS9I GGNCC 5 cut(s) 640, 982, 1096, 1337, 1530
AsuC2I CCSGG 2 cut(s) 985, 986
AsuHPI GGTGA 1 cut(s) 818
AvaI CYCGRG 2 cut(s) 984, 1484
AvaII GGWCC 2 cut(s) 1096, 1337
BaeGI GKGCMC 3 cut(s) 48, 254, 864
BanI GGYRCC 1 cut(s) 1043
BanII GRGCYC 2 cut(s) 139, 811
BauI CACGAG 1 cut(s) 94
Bbv12I GWGCWC 3 cut(s) 48, 864, 1523
BbvI GCAGC 9 cut(s) 43, 70, 73, 76, 506, 758, 761, 868, 939
BccI CCATC 4 cut(s) 8, 1034, 1350, 1358
BceAI ACGGC 1 cut(s) 1347
BcgI CGANNNNNNTGC 2 cut(s) 1000, 1034
BciT130I CCWGG 4 cut(s) 502, 1129, 1309, 1491
BciVI GTATCC 1 cut(s) 1016
BcnI CCSGG 2 cut(s) 985, 986
BcoDI GTCTC 3 cut(s) 449, 782, 1362
BfaI CTAG 6 cut(s) 219, 819, 857, 896, 1269, 1382
BfmI CTRYAG 1 cut(s) 1141
BfrI CTTAAG 1 cut(s) 1544
BfuI GTATCC 1 cut(s) 1016
BlpI GCTNAGC 1 cut(s) 852
Bme1390I CCNGG 6 cut(s) 502, 985, 986, 1129, 1309, 1491
Bme18I GGWCC 2 cut(s) 1096, 1337
BmeT110I CYCGRG 2 cut(s) 984, 1484
BmgBI CACGTC 1 cut(s) 625
BmgT120I GGNCC 5 cut(s) 640, 982, 1096, 1337, 1530
BmiI GGNNCC 3 cut(s) 416, 1045, 1125
BmrFI CCNGG 6 cut(s) 502, 985, 986, 1129, 1309, 1491
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 7 cut(s) 229, 374, 396, 766, 868, 893, 1162
BmuI ACTGGG 1 cut(s) 236
BoxI GACNNNNGTC 1 cut(s) 794
Bpu10I CCTNAGC 3 cut(s) 123, 812, 1550
Bpu1102I GCTNAGC 1 cut(s) 852
BpuEI CTTGAG 2 cut(s) 304, 1438
BpuMI CCSGG 2 cut(s) 985, 986
BsaAI YACGTR 1 cut(s) 358
BsaHI GRCGYC 1 cut(s) 795
BsaJI CCNNGG 4 cut(s) 984, 1127, 1434, 1526
Bsc4I CCNNNNNNNGG 1 cut(s) 1015
Bse118I RCCGGY 1 cut(s) 634
Bse1I ACTGG 1 cut(s) 231
BseBI CCWGG 4 cut(s) 502, 1129, 1309, 1491
BseDI CCNNGG 4 cut(s) 984, 1127, 1434, 1526
BseGI GGATG 9 cut(s) 244, 387, 869, 928, 994, 1045, 1081, 1361, 1369
BseLI CCNNNNNNNGG 1 cut(s) 1015
BseMII CTCAG 2 cut(s) 406, 843
BseNI ACTGG 1 cut(s) 231
BseRI GAGGAG 2 cut(s) 667, 1049
BseSI GKGCMC 3 cut(s) 48, 254, 864
BseX3I CGGCCG 1 cut(s) 178
BseXI GCAGC 9 cut(s) 43, 70, 73, 76, 506, 758, 761, 868, 939
BseYI CCCAGC 2 cut(s) 255, 1532
BsgI GTGCAG 2 cut(s) 888, 1303
Bsh1236I CGCG 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 5 cut(s) 72, 180, 641, 983, 1531
BshNI GGYRCC 1 cut(s) 1043
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 3 cut(s) 48, 864, 1523
BsiHKCI CYCGRG 2 cut(s) 984, 1484
BsiSI CCGG 4 cut(s) 109, 177, 635, 985
BslFI GGGAC 1 cut(s) 642
BslI CCNNNNNNNGG 1 cut(s) 1015
BsmAI GTCTC 3 cut(s) 449, 782, 1362
BsmBI CGTCTC 1 cut(s) 782
BsmFI GGGAC 1 cut(s) 642
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 5 cut(s) 72, 180, 641, 983, 1531
BsoBI CYCGRG 2 cut(s) 984, 1484
Bsp1286I GDGCHC 6 cut(s) 48, 139, 254, 811, 864, 1523
Bsp143I GATC 6 cut(s) 4, 340, 522, 1087, 1387, 1398
Bsp1720I GCTNAGC 1 cut(s) 852
BspACI CCGC 5 cut(s) 181, 690, 849, 1003, 1009
BspANI GGCC 5 cut(s) 72, 180, 641, 983, 1531
BspCNI CTCAG 2 cut(s) 405, 844
BspFNI CGCG 1 cut(s) 183
BspLI GGNNCC 3 cut(s) 416, 1045, 1125
BspPI GGATC 3 cut(s) 12, 530, 1095
BspQI GCTCTTC 1 cut(s) 1198
BspT107I GGYRCC 1 cut(s) 1043
BspTI CTTAAG 1 cut(s) 1544
BsrFI RCCGGY 1 cut(s) 634
BsrI ACTGG 1 cut(s) 231
BssAI RCCGGY 1 cut(s) 634
BssECI CCNNGG 4 cut(s) 984, 1127, 1434, 1526
BssMI GATC 6 cut(s) 4, 340, 522, 1087, 1387, 1398
BssNI GRCGYC 1 cut(s) 795
BssSI CACGAG 1 cut(s) 94
BssT1I CCWWGG 2 cut(s) 1434, 1526
Bst2BI CACGAG 1 cut(s) 94
Bst2UI CCWGG 4 cut(s) 502, 1129, 1309, 1491
Bst4CI ACNGT 4 cut(s) 43, 706, 833, 1245
Bst6I CTCTTC 2 cut(s) 1077, 1198
BstACI GRCGYC 1 cut(s) 795
BstAFI CTTAAG 1 cut(s) 1544
BstAPI GCANNNNNTGC 1 cut(s) 755
BstBAI YACGTR 1 cut(s) 358
BstC8I GCNNGC 1 cut(s) 1292
BstDEI CTNAG 6 cut(s) 123, 392, 459, 812, 852, 1550
BstEII GGTNACC 1 cut(s) 824
BstF5I GGATG 9 cut(s) 244, 387, 869, 928, 994, 1045, 1081, 1361, 1369
BstFNI CGCG 1 cut(s) 183
BstKTI GATC 6 cut(s) 7, 343, 525, 1090, 1390, 1401
BstMAI GTCTC 3 cut(s) 449, 782, 1362
BstMBI GATC 6 cut(s) 4, 340, 522, 1087, 1387, 1398
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 8 cut(s) 89, 105, 134, 335, 755, 1008, 1181, 1462
BstNI CCWGG 4 cut(s) 502, 1129, 1309, 1491
BstNSI RCATGY 2 cut(s) 841, 1465
BstPAI GACNNNNGTC 1 cut(s) 794
BstPI GGTNACC 1 cut(s) 824
BstSCI CCNGG 6 cut(s) 500, 983, 984, 1127, 1307, 1489
BstSFI CTRYAG 1 cut(s) 1141
BstSLI GKGCMC 3 cut(s) 48, 254, 864
BstSNI TACGTA 1 cut(s) 358
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 9 cut(s) 43, 70, 73, 76, 506, 758, 761, 868, 939
BstXI CCANNNNNNTGG 1 cut(s) 17
BstZI CGGCCG 1 cut(s) 178
BsuI GTATCC 1 cut(s) 1016
BsuRI GGCC 5 cut(s) 72, 180, 641, 983, 1531
BtgZI GCGATG 1 cut(s) 873
BtrI CACGTC 1 cut(s) 625
BtsCI GGATG 9 cut(s) 244, 387, 869, 928, 994, 1045, 1081, 1361, 1369
BtsI GCAGTG 3 cut(s) 591, 1233, 1584
BtsIMutI CAGTG 7 cut(s) 48, 591, 702, 838, 873, 1233, 1584
Cac8I GCNNGC 1 cut(s) 1292
Cfr10I RCCGGY 1 cut(s) 634
Cfr13I GGNCC 5 cut(s) 640, 982, 1096, 1337, 1530
Cfr9I CCCGGG 1 cut(s) 984
Csp6I GTAC 1 cut(s) 618
CspCI CAANNNNNGTGG 2 cut(s) 741, 776
CviAII CATG 9 cut(s) 11, 484, 838, 931, 948, 979, 1012, 1213, 1462
CviQI GTAC 1 cut(s) 618
DdeI CTNAG 6 cut(s) 123, 392, 459, 812, 852, 1550
DpnI GATC 6 cut(s) 6, 342, 524, 1089, 1389, 1400
DpnII GATC 6 cut(s) 4, 340, 522, 1087, 1387, 1398
EaeI YGGCCR 1 cut(s) 178
EagI CGGCCG 1 cut(s) 178
Eam1104I CTCTTC 2 cut(s) 1077, 1198
EarI CTCTTC 2 cut(s) 1077, 1198
EclXI CGGCCG 1 cut(s) 178
Eco105I TACGTA 1 cut(s) 358
Eco130I CCWWGG 2 cut(s) 1434, 1526
Eco24I GRGCYC 2 cut(s) 139, 811
Eco47I GGWCC 2 cut(s) 1096, 1337
Eco52I CGGCCG 1 cut(s) 178
Eco57I CTGAAG 2 cut(s) 315, 1523
Eco88I CYCGRG 2 cut(s) 984, 1484
Eco91I GGTNACC 1 cut(s) 824
EcoO65I GGTNACC 1 cut(s) 824
EcoRI GAATTC 1 cut(s) 1249
EcoRII CCWGG 4 cut(s) 500, 1127, 1307, 1489
EcoT14I CCWWGG 2 cut(s) 1434, 1526
EcoT22I ATGCAT 1 cut(s) 389
EcoT38I GRGCYC 2 cut(s) 139, 811
ErhI CCWWGG 2 cut(s) 1434, 1526
Esp3I CGTCTC 1 cut(s) 782
FaeI CATG 9 cut(s) 14, 487, 841, 934, 951, 982, 1015, 1216, 1465
FaqI GGGAC 1 cut(s) 642
FatI CATG 9 cut(s) 10, 483, 837, 930, 947, 978, 1011, 1212, 1461
FokI GGATG 9 cut(s) 251, 374, 856, 935, 1001, 1052, 1088, 1368, 1376
FriOI GRGCYC 2 cut(s) 139, 811
FspBI CTAG 6 cut(s) 219, 819, 857, 896, 1269, 1382
GsaI CCCAGC 2 cut(s) 259, 1536
HaeIII GGCC 5 cut(s) 72, 180, 641, 983, 1531
HapII CCGG 4 cut(s) 109, 177, 635, 985
Hin1I GRCGYC 1 cut(s) 795
Hin1II CATG 9 cut(s) 14, 487, 841, 934, 951, 982, 1015, 1216, 1465
HincII GTYRAC 2 cut(s) 34, 1300
HindII GTYRAC 2 cut(s) 34, 1300
HinfI GANTC 6 cut(s) 173, 201, 347, 655, 728, 1442
HpaII CCGG 4 cut(s) 109, 177, 635, 985
HphI GGTGA 1 cut(s) 818
Hpy166II GTNNAC 6 cut(s) 34, 46, 862, 994, 1241, 1300
Hpy188I TCNGA 2 cut(s) 334, 1022
Hpy188III TCNNGA 8 cut(s) 344, 741, 819, 1094, 1219, 1391, 1484, 1501
Hpy8I GTNNAC 6 cut(s) 34, 46, 862, 994, 1241, 1300
HpyAV CCTTC 5 cut(s) 83, 908, 912, 1354, 1547
HpyCH4III ACNGT 4 cut(s) 43, 706, 833, 1245
HpyCH4IV ACGT 3 cut(s) 357, 624, 795
HpyF10VI GCNNNNNNNGC 8 cut(s) 89, 105, 134, 335, 755, 1008, 1181, 1462
HpyF3I CTNAG 6 cut(s) 123, 392, 459, 812, 852, 1550
HpySE526I ACGT 3 cut(s) 357, 624, 795
Hsp92I GRCGYC 1 cut(s) 795
Hsp92II CATG 9 cut(s) 14, 487, 841, 934, 951, 982, 1015, 1216, 1465
Kzo9I GATC 6 cut(s) 4, 340, 522, 1087, 1387, 1398
LguI GCTCTTC 1 cut(s) 1198
LmnI GCTCC 5 cut(s) 414, 532, 743, 846, 1526
Lsp1109I GCAGC 9 cut(s) 43, 70, 73, 76, 506, 758, 761, 868, 939
LweI GCATC 7 cut(s) 229, 374, 396, 766, 868, 893, 1162
MaeI CTAG 6 cut(s) 219, 819, 857, 896, 1269, 1382
MaeII ACGT 3 cut(s) 357, 624, 795
MaeIII GTNAC 3 cut(s) 824, 833, 898
MalI GATC 6 cut(s) 6, 342, 524, 1089, 1389, 1400
MboI GATC 6 cut(s) 4, 340, 522, 1087, 1387, 1398
MboII GAAGA 7 cut(s) 317, 475, 659, 1091, 1094, 1117, 1215
MhlI GDGCHC 6 cut(s) 48, 139, 254, 811, 864, 1523
MluCI AATT 7 cut(s) 361, 374, 1249, 1423, 1569, 1575, 1595
MlyI GAGTC 1 cut(s) 664
MmeI TCCRAC 2 cut(s) 1000, 1138
Mph1103I ATGCAT 1 cut(s) 389
MseI TTAA 4 cut(s) 369, 1545, 1563, 1603
MslI CAYNNNNRTG 3 cut(s) 12, 15, 831
MspA1I CMGCKG 1 cut(s) 851
MspCI CTTAAG 1 cut(s) 1544
MspI CCGG 4 cut(s) 109, 177, 635, 985
MspR9I CCNGG 6 cut(s) 502, 985, 986, 1129, 1309, 1491
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 4 cut(s) 502, 1129, 1309, 1491
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 8 cut(s) 89, 105, 134, 335, 755, 1008, 1181, 1462
NciI CCSGG 2 cut(s) 985, 986
NdeII GATC 6 cut(s) 4, 340, 522, 1087, 1387, 1398
NlaIII CATG 9 cut(s) 14, 487, 841, 934, 951, 982, 1015, 1216, 1465
NlaIV GGNNCC 3 cut(s) 416, 1045, 1125
NmuCI GTSAC 3 cut(s) 824, 833, 898
NsiI ATGCAT 1 cut(s) 389
NspI RCATGY 2 cut(s) 841, 1465
OliI CACNNNNGTG 1 cut(s) 831
PaeR7I CTCGAG 1 cut(s) 1484
PciSI GCTCTTC 1 cut(s) 1198
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PctI GAATGC 1 cut(s) 454
PfeI GAWTC 5 cut(s) 173, 201, 347, 728, 1442
PleI GAGTC 1 cut(s) 663
PpsI GAGTC 1 cut(s) 663
Ppu21I YACGTR 1 cut(s) 358
PshAI GACNNNNGTC 1 cut(s) 794
Psp6I CCWGG 4 cut(s) 500, 1127, 1307, 1489
PspEI GGTNACC 1 cut(s) 824
PspFI CCCAGC 2 cut(s) 255, 1532
PspGI CCWGG 4 cut(s) 500, 1127, 1307, 1489
PspN4I GGNNCC 3 cut(s) 416, 1045, 1125
PspPI GGNCC 5 cut(s) 640, 982, 1096, 1337, 1530
RsaI GTAC 1 cut(s) 619
RsaNI GTAC 1 cut(s) 618
RseI CAYNNNNRTG 3 cut(s) 12, 15, 831
SapI GCTCTTC 1 cut(s) 1198
SaqAI TTAA 4 cut(s) 369, 1545, 1563, 1603
Sau3AI GATC 6 cut(s) 4, 340, 522, 1087, 1387, 1398
Sau96I GGNCC 5 cut(s) 640, 982, 1096, 1337, 1530
SchI GAGTC 1 cut(s) 664
ScrFI CCNGG 6 cut(s) 502, 985, 986, 1129, 1309, 1491
SduI GDGCHC 6 cut(s) 48, 139, 254, 811, 864, 1523
SfaNI GCATC 7 cut(s) 229, 374, 396, 766, 868, 893, 1162
SfcI CTRYAG 1 cut(s) 1141
Sfr274I CTCGAG 1 cut(s) 1484
SinI GGWCC 2 cut(s) 1096, 1337
SlaI CTCGAG 1 cut(s) 1484
SmaI CCCGGG 1 cut(s) 986
SmiMI CAYNNNNRTG 3 cut(s) 12, 15, 831
SmlI CTYRAG 4 cut(s) 283, 1417, 1484, 1544
SmoI CTYRAG 4 cut(s) 283, 1417, 1484, 1544
SnaBI TACGTA 1 cut(s) 358
Sse9I AATT 7 cut(s) 361, 374, 1249, 1423, 1569, 1575, 1595
SsiI CCGC 5 cut(s) 181, 690, 849, 1003, 1009
SspI AATATT 1 cut(s) 323
SspMI CTAG 6 cut(s) 219, 819, 857, 896, 1269, 1382
StyD4I CCNGG 6 cut(s) 500, 983, 984, 1127, 1307, 1489
StyI CCWWGG 2 cut(s) 1434, 1526
TaaI ACNGT 4 cut(s) 43, 706, 833, 1245
TaiI ACGT 3 cut(s) 360, 627, 798
TaqI TCGA 3 cut(s) 195, 1386, 1485
TasI AATT 7 cut(s) 361, 374, 1249, 1423, 1569, 1575, 1595
TauI GCSGC 2 cut(s) 183, 1005
TfiI GAWTC 5 cut(s) 173, 201, 347, 728, 1442
Tru1I TTAA 4 cut(s) 369, 1545, 1563, 1603
Tru9I TTAA 4 cut(s) 369, 1545, 1563, 1603
TscAI CASTG 7 cut(s) 48, 598, 709, 838, 880, 1233, 1591
TseFI GTSAC 3 cut(s) 824, 833, 898
TseI GCWGC 9 cut(s) 56, 83, 86, 89, 494, 746, 749, 881, 952
Tsp45I GTSAC 3 cut(s) 824, 833, 898
TspDTI ATGAA 8 cut(s) 398, 585, 590, 644, 927, 1092, 1263, 1569
TspGWI ACGGA 3 cut(s) 185, 635, 806
TspMI CCCGGG 1 cut(s) 984
TspRI CASTG 7 cut(s) 48, 598, 709, 838, 880, 1233, 1591
Vha464I CTTAAG 1 cut(s) 1544
VneI GTGCAC 2 cut(s) 44, 860
VpaK11BI GGWCC 2 cut(s) 1096, 1337
XapI RAATTY 4 cut(s) 361, 374, 1249, 1575
XbaI TCTAGA 1 cut(s) 818
XceI RCATGY 2 cut(s) 841, 1465
XhoI CTCGAG 1 cut(s) 1484
XmaI CCCGGG 1 cut(s) 984
XspI CTAG 6 cut(s) 219, 819, 857, 896, 1269, 1382
ZraI GACGTC 1 cut(s) 796
Zsp2I ATGCAT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.