pycom16g17890

Protein of unknown function (DUF668)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014582
Physical Location & Seq
Reverse (-)
14258014 .. 14259393
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g17890.1

Sequence Viewer

Length: 1380 bp
ATGGCGGAGAAACTGGAGGAGCTGAACGGGGTCGCTGGCGTCGTGGGGAGGCTCGGAAAGCGGTGCGTCGAGCCGGCTCTGCAGGGGTTCGAGCATGTCTATGCCGACCTCGTTAATGGGGTTATCGATGTGAAGGAGTTGGGGTTTTTGGTAAAAGACATGGAGGGCATGGTGAGAAAAATGGAGAGGTATGTGAGTGCAACTTCGAATTTGTACAGCGAAATGGAGGTTTTGAATGAGTTGGAACAAGCCACCAAGAAGTTTCAGAACAATCAGCACGAGGAGAGCAAAAGGGCTTTTGAGCAGAAGCTCGTTTGGCAGAAGCAGGATGTCAAGCATCTCAAGGATGTCTCTCTTTGGAACCAAACGTATGATAAAGTTGTGGAATTGCTGGCCAGGACGGTCTGTACGATTTTCGCCACAATTCAGGCTGTTTTTGGTGACTCTGTTTTGTGCAAGGACCGTGTTGGGATGATTGGTGGAGCTTCACCTCCTCCGGTGTCCGGTCAGACTGATGTGAGGAGGGTTTCTCAGGTGGCTTCTGAGCCATTGAAGAGGGTTGCTAGTAGGAAAAATGGGTTGCATTCGGGTCCAGTTGAGAAAGCCGTGGTGTTGAAAAAGGGGCCATCCTTTAAGCCTCAGTTTGATTCGAGGAGAGGAGAATTGGCAGCGTTTCGAGCTGAGGATTTGAATTTTCTGTGTGGAGCAAGCCCCGGGAGGATTTTCATGGACTGCCTAAGAGTGAGCGGTACAGTTGATGATGATTATGGTGGTGCTGGTAATTATGATGACAGGAGTAGCCAAATTTCGGGTTGCAGTGTTGCAAATGGTGGTTTGAAGAGAGAGTTTCCGTACCATTCAGGTTGTTTTAATCGAGTACAAATGAATGTTCAACTTATATCCAAGTGTAGCGGTGCGAATGATGGTGCACGGTTTGGTCCCAAGAGTAAGTTAATGGTTTATACTCCTCCTTCTACTGTGGGGGGCTCTGCTCTAGCTTTACATTATGCAAATGTCATAATTGTTATAGAGAAGTTGCTTCGATATCCTTATCTAGTGGGCGAGGAAGCGAGGGACGATTTGTATCATATGTTACCAATGAGTTTAAGGATGGGTCTGAGGACTAATCTCAAGTCCTATGCCAAGAATATGGCCATATATGATGCGCCTCTTGCGCATGATTGGAAGGACACCCTTGACGGGATTTTGAGGTGGCTCGCCCCATTGGCGCACAACATGATCAGATGGCAGAGTGAGCGCAATTTTGAGCAAACGCAGATTTTTACGCGGACAAATGTTCTTCTGCTTCAGACATTGTATTTTGCTCGATCGATCGAGAGAAGACAGAGGCAGCTATTTGTAAGCTACTTGTTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

460

Amino Acids

51.47

Weight (kDa)

9.0

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF668 PF05003 326 - 415 4.5e-34 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23160
fragaria_vesca FvH4_4g03090
malus_domestica MD13G1210100.v1.1 MD16G1213000.v1.1
prunus_persica Prupe.1G034600_v2.0.a1
pyrus_communis pycom13g18270 pycom16g17890
rosa_chinensis RchiOBHm_Chr4g0391361
rosa_laevigata RLG00000009869
rosa_multiflora Rmu_sc0005322.1_g000036
rosa_roxburghii Rroxscaffold_5G00337110
rosa_rugosa Rorug03G0335700
rosa_samantha Rh4AG036800 Rh4BG031500 Rh4CG040100 Rh4DG034500
rosa_wichuraiana Rw4G002890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1176
AccBSI CCGCTC 1 cut(s) 747
AccII CGCG 1 cut(s) 1288
AciI CCGC 5 cut(s) 5, 61, 747, 912, 1288
AcoI YGGCCR 2 cut(s) 393, 1152
AcsI RAATTY 3 cut(s) 208, 691, 804
AcuI CTGAAG 1 cut(s) 1292
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 5 cut(s) 215, 409, 751, 854, 879
AfiI CCNNNNNNNGG 3 cut(s) 503, 808, 1200
AgsI TTSAA 6 cut(s) 235, 553, 616, 691, 838, 893
AjnI CCWGG 1 cut(s) 395
AluBI AGCT 7 cut(s) 22, 310, 485, 680, 998, 1354, 1365
AluI AGCT 7 cut(s) 22, 310, 485, 680, 998, 1354, 1365
Alw21I GWGCWC 1 cut(s) 931
Alw26I GTCTC 1 cut(s) 355
Alw44I GTGCAC 1 cut(s) 927
Ama87I CYCGRG 1 cut(s) 713
AoxI GGCC 3 cut(s) 393, 623, 1152
ApaLI GTGCAC 1 cut(s) 927
ApeKI GCWGC 2 cut(s) 668, 1351
ApoI RAATTY 3 cut(s) 208, 691, 804
AspLEI GCGC 4 cut(s) 1168, 1177, 1231, 1260
AspS9I GGNCC 4 cut(s) 460, 590, 623, 938
AsuC2I CCSGG 2 cut(s) 714, 715
AsuHPI GGTGA 3 cut(s) 184, 452, 480
AsuII TTCGAA 1 cut(s) 206
AvaI CYCGRG 1 cut(s) 713
AvaII GGWCC 3 cut(s) 460, 590, 938
BaeGI GKGCMC 1 cut(s) 931
BalI TGGCCA 2 cut(s) 395, 1154
BanII GRGCYC 1 cut(s) 989
BauI CACGAG 1 cut(s) 278
BbsI GAAGAC 1 cut(s) 1348
Bbv12I GWGCWC 1 cut(s) 931
BbvCI CCTCAGC 1 cut(s) 681
BbvI GCAGC 2 cut(s) 680, 1363
BccI CCATC 4 cut(s) 634, 917, 1105, 1239
BceAI ACGGC 1 cut(s) 590
BciT130I CCWGG 1 cut(s) 397
BclI TGATCA 1 cut(s) 1239
BcnI CCSGG 2 cut(s) 714, 715
BcoDI GTCTC 1 cut(s) 355
BfaI CTAG 3 cut(s) 564, 995, 1055
BfmI CTRYAG 1 cut(s) 80
BglI GCCNNNNNGGC 1 cut(s) 1226
BisI GCNGC 2 cut(s) 669, 1352
BlsI GCNGC 2 cut(s) 670, 1353
Bme1390I CCNGG 3 cut(s) 397, 714, 715
Bme18I GGWCC 3 cut(s) 460, 590, 938
BmeT110I CYCGRG 1 cut(s) 713
BmgT120I GGNCC 4 cut(s) 460, 590, 623, 938
BmiI GGNNCC 4 cut(s) 362, 591, 624, 940
BmrFI CCNGG 3 cut(s) 397, 714, 715
BmsI GCATC 2 cut(s) 346, 1153
BpiI GAAGAC 1 cut(s) 1348
BplI GAGNNNNNCTC 2 cut(s) 514, 546
BpmI CTGGAG 1 cut(s) 35
Bpu10I CCTNAGC 1 cut(s) 681
Bpu14I TTCGAA 1 cut(s) 206
BpuEI CTTGAG 2 cut(s) 326, 1115
BpuMI CCSGG 2 cut(s) 714, 715
Bsa29I ATCGAT 2 cut(s) 126, 1331
BsaBI GATNNNNATC 1 cut(s) 1083
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 3 cut(s) 606, 712, 713
BsaWI WCCGGW 2 cut(s) 496, 503
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 3 cut(s) 503, 808, 1200
Bse118I RCCGGY 1 cut(s) 73
Bse1I ACTGG 2 cut(s) 18, 593
Bse8I GATNNNNATC 1 cut(s) 1083
BseBI CCWGG 1 cut(s) 397
BseCI ATCGAT 2 cut(s) 126, 1331
BseDI CCNNGG 3 cut(s) 606, 712, 713
BseGI GGATG 5 cut(s) 334, 352, 477, 626, 1116
BseJI GATNNNNATC 1 cut(s) 1083
BseLI CCNNNNNNNGG 3 cut(s) 503, 808, 1200
BseMII CTCAG 5 cut(s) 534, 545, 653, 672, 1109
BseNI ACTGG 2 cut(s) 18, 593
BseRI GAGGAG 7 cut(s) 32, 296, 483, 535, 667, 672, 957
BseSI GKGCMC 1 cut(s) 931
BseXI GCAGC 2 cut(s) 680, 1363
Bsh1236I CGCG 1 cut(s) 1288
Bsh1285I CGRYCG 2 cut(s) 1331, 1335
BshFI GGCC 3 cut(s) 395, 625, 1154
BshVI ATCGAT 2 cut(s) 126, 1331
BsiEI CGRYCG 2 cut(s) 1331, 1335
BsiHKAI GWGCWC 1 cut(s) 931
BsiHKCI CYCGRG 1 cut(s) 713
BsiSI CCGG 4 cut(s) 74, 497, 504, 714
BslFI GGGAC 2 cut(s) 924, 1088
BslI CCNNNNNNNGG 3 cut(s) 503, 808, 1200
BsmAI GTCTC 1 cut(s) 355
BsmFI GGGAC 2 cut(s) 924, 1088
BsmI GAATGC 1 cut(s) 583
BsnI GGCC 3 cut(s) 395, 625, 1154
BsoBI CYCGRG 1 cut(s) 713
Bsp119I TTCGAA 1 cut(s) 206
Bsp1286I GDGCHC 2 cut(s) 931, 989
Bsp1407I TGTACA 1 cut(s) 213
Bsp143I GATC 3 cut(s) 1239, 1328, 1332
BspACI CCGC 5 cut(s) 5, 61, 747, 912, 1288
BspANI GGCC 3 cut(s) 395, 625, 1154
BspCNI CTCAG 5 cut(s) 535, 544, 652, 673, 1110
BspDI ATCGAT 2 cut(s) 126, 1331
BspFNI CGCG 1 cut(s) 1288
BspLI GGNNCC 4 cut(s) 362, 591, 624, 940
BspMAI CTGCAG 1 cut(s) 84
BspT104I TTCGAA 1 cut(s) 206
BsrBI CCGCTC 1 cut(s) 747
BsrFI RCCGGY 1 cut(s) 73
BsrGI TGTACA 1 cut(s) 213
BsrI ACTGG 2 cut(s) 18, 593
BssAI RCCGGY 1 cut(s) 73
BssECI CCNNGG 3 cut(s) 606, 712, 713
BssMI GATC 3 cut(s) 1239, 1328, 1332
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 278
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 1 cut(s) 397
Bst4CI ACNGT 5 cut(s) 403, 464, 754, 933, 979
Bst6I CTCTTC 2 cut(s) 548, 833
BstACI GRCGYC 1 cut(s) 39
BstAUI TGTACA 1 cut(s) 213
BstBI TTCGAA 1 cut(s) 206
BstC8I GCNNGC 5 cut(s) 37, 75, 393, 709, 1218
BstDEI CTNAG 6 cut(s) 531, 543, 639, 681, 737, 1118
BstDSI CCRYGG 1 cut(s) 606
BstF5I GGATG 5 cut(s) 334, 352, 477, 626, 1116
BstFNI CGCG 1 cut(s) 1288
BstHHI GCGC 4 cut(s) 1168, 1177, 1231, 1260
BstKTI GATC 3 cut(s) 1242, 1331, 1335
BstMAI GTCTC 1 cut(s) 355
BstMBI GATC 3 cut(s) 1239, 1328, 1332
BstMCI CGRYCG 2 cut(s) 1331, 1335
BstMWI GCNNNNNNNGC 8 cut(s) 58, 79, 316, 677, 1172, 1174, 1226, 1255
BstNI CCWGG 1 cut(s) 397
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 3 cut(s) 395, 712, 713
BstSFI CTRYAG 1 cut(s) 80
BstSLI GKGCMC 1 cut(s) 931
BstUI CGCG 1 cut(s) 1288
BstV1I GCAGC 2 cut(s) 680, 1363
BstV2I GAAGAC 1 cut(s) 1348
BstXI CCANNNNNNTGG 1 cut(s) 1150
Bsu15I ATCGAT 2 cut(s) 126, 1331
BsuRI GGCC 3 cut(s) 395, 625, 1154
BsuTUI ATCGAT 2 cut(s) 126, 1331
BtgI CCRYGG 1 cut(s) 606
BtsCI GGATG 5 cut(s) 334, 352, 477, 626, 1116
BtsI GCAGTG 1 cut(s) 823
BtsIMutI CAGTG 1 cut(s) 823
Cac8I GCNNGC 5 cut(s) 37, 75, 393, 709, 1218
CfoI GCGC 4 cut(s) 1168, 1177, 1231, 1260
Cfr10I RCCGGY 1 cut(s) 73
Cfr13I GGNCC 4 cut(s) 460, 590, 623, 938
Cfr9I CCCGGG 1 cut(s) 713
ClaI ATCGAT 2 cut(s) 126, 1331
CseI GACGC 2 cut(s) 28, 55
Csp6I GTAC 5 cut(s) 214, 408, 750, 853, 878
CviAII CATG 6 cut(s) 95, 160, 169, 727, 1178, 1237
CviQI GTAC 5 cut(s) 214, 408, 750, 853, 878
DdeI CTNAG 6 cut(s) 531, 543, 639, 681, 737, 1118
DpnI GATC 3 cut(s) 1241, 1330, 1334
DpnII GATC 3 cut(s) 1239, 1328, 1332
EaeI YGGCCR 2 cut(s) 393, 1152
Eam1104I CTCTTC 2 cut(s) 548, 833
EarI CTCTTC 2 cut(s) 548, 833
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 989
Eco32I GATATC 1 cut(s) 1046
Eco47I GGWCC 3 cut(s) 460, 590, 938
Eco57I CTGAAG 1 cut(s) 1292
Eco88I CYCGRG 1 cut(s) 713
EcoRII CCWGG 1 cut(s) 395
EcoRV GATATC 1 cut(s) 1046
EcoT38I GRGCYC 1 cut(s) 989
FaeI CATG 6 cut(s) 98, 163, 172, 730, 1181, 1240
FaqI GGGAC 2 cut(s) 924, 1088
FatI CATG 6 cut(s) 94, 159, 168, 726, 1177, 1236
FauNDI CATATG 1 cut(s) 1089
FbaI TGATCA 1 cut(s) 1239
Fnu4HI GCNGC 2 cut(s) 669, 1352
FokI GGATG 5 cut(s) 341, 359, 484, 613, 1123
FriOI GRGCYC 1 cut(s) 989
Fsp4HI GCNGC 2 cut(s) 669, 1352
FspBI CTAG 3 cut(s) 564, 995, 1055
FspI TGCGCA 1 cut(s) 1176
GlaI GCGC 4 cut(s) 1167, 1176, 1230, 1259
GluI GCNGC 2 cut(s) 669, 1352
GsuI CTGGAG 1 cut(s) 35
HaeIII GGCC 3 cut(s) 395, 625, 1154
HapII CCGG 4 cut(s) 74, 497, 504, 714
HgaI GACGC 2 cut(s) 28, 55
HhaI GCGC 4 cut(s) 1168, 1177, 1231, 1260
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 6 cut(s) 98, 163, 172, 730, 1181, 1240
Hin6I GCGC 4 cut(s) 1166, 1175, 1229, 1258
HinP1I GCGC 4 cut(s) 1166, 1175, 1229, 1258
HinfI GANTC 2 cut(s) 443, 647
HpaII CCGG 4 cut(s) 74, 497, 504, 714
HphI GGTGA 3 cut(s) 184, 452, 480
Hpy166II GTNNAC 1 cut(s) 929
Hpy188I TCNGA 7 cut(s) 56, 267, 510, 544, 1119, 1244, 1311
Hpy188III TCNNGA 1 cut(s) 1336
Hpy8I GTNNAC 1 cut(s) 929
Hpy99I CGWCG 2 cut(s) 44, 71
HpyAV CCTTC 3 cut(s) 127, 981, 1180
HpyCH4III ACNGT 5 cut(s) 403, 464, 754, 933, 979
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 8 cut(s) 82, 200, 456, 583, 816, 824, 929, 1010
HpyF10VI GCNNNNNNNGC 8 cut(s) 58, 79, 316, 677, 1172, 1174, 1226, 1255
HpyF3I CTNAG 6 cut(s) 531, 543, 639, 681, 737, 1118
HpySE526I ACGT 1 cut(s) 368
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 6 cut(s) 98, 163, 172, 730, 1181, 1240
HspAI GCGC 4 cut(s) 1166, 1175, 1229, 1258
KroI GCCGGC 1 cut(s) 73
KroNI GCCGGC 1 cut(s) 75
Ksp22I TGATCA 1 cut(s) 1239
Kzo9I GATC 3 cut(s) 1239, 1328, 1332
LmnI GCTCC 3 cut(s) 19, 482, 704
Lsp1109I GCAGC 2 cut(s) 680, 1363
LweI GCATC 2 cut(s) 346, 1153
MaeI CTAG 3 cut(s) 564, 995, 1055
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 2 cut(s) 440, 1092
MalI GATC 3 cut(s) 1241, 1330, 1334
MbiI CCGCTC 1 cut(s) 747
MboI GATC 3 cut(s) 1239, 1328, 1332
MboII GAAGA 4 cut(s) 565, 850, 1292, 1353
MhlI GDGCHC 2 cut(s) 931, 989
MlsI TGGCCA 2 cut(s) 395, 1154
MluCI AATT 9 cut(s) 208, 386, 423, 662, 691, 781, 804, 1020, 1261
MluNI TGGCCA 2 cut(s) 395, 1154
MlyI GAGTC 1 cut(s) 437
MmeI TCCRAC 1 cut(s) 222
Mox20I TGGCCA 2 cut(s) 395, 1154
MroNI GCCGGC 1 cut(s) 73
MscI TGGCCA 2 cut(s) 395, 1154
MseI TTAA 5 cut(s) 114, 633, 870, 953, 1106
MslI CAYNNNNRTG 1 cut(s) 99
Msp20I TGGCCA 2 cut(s) 395, 1154
MspI CCGG 4 cut(s) 74, 497, 504, 714
MspR9I CCNGG 3 cut(s) 397, 714, 715
Mva1269I GAATGC 1 cut(s) 583
MvaI CCWGG 1 cut(s) 397
MvnI CGCG 1 cut(s) 1288
MwoI GCNNNNNNNGC 8 cut(s) 58, 79, 316, 677, 1172, 1174, 1226, 1255
NaeI GCCGGC 1 cut(s) 75
NciI CCSGG 2 cut(s) 714, 715
NdeI CATATG 1 cut(s) 1089
NdeII GATC 3 cut(s) 1239, 1328, 1332
NgoMIV GCCGGC 1 cut(s) 73
NlaIII CATG 6 cut(s) 98, 163, 172, 730, 1181, 1240
NlaIV GGNNCC 4 cut(s) 362, 591, 624, 940
NmuCI GTSAC 1 cut(s) 440
NsbI TGCGCA 1 cut(s) 1176
NspI RCATGY 1 cut(s) 98
NspV TTCGAA 1 cut(s) 206
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 407
PctI GAATGC 1 cut(s) 583
PdiI GCCGGC 1 cut(s) 75
PfeI GAWTC 1 cut(s) 647
PkrI GCNGC 2 cut(s) 670, 1353
Ple19I CGATCG 2 cut(s) 1331, 1335
PleI GAGTC 1 cut(s) 437
PpsI GAGTC 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 395
PspGI CCWGG 1 cut(s) 395
PspN4I GGNNCC 4 cut(s) 362, 591, 624, 940
PspPI GGNCC 4 cut(s) 460, 590, 623, 938
PstI CTGCAG 1 cut(s) 84
PvuI CGATCG 2 cut(s) 1331, 1335
RsaI GTAC 5 cut(s) 215, 409, 751, 854, 879
RsaNI GTAC 5 cut(s) 214, 408, 750, 853, 878
RseI CAYNNNNRTG 1 cut(s) 99
SaqAI TTAA 5 cut(s) 114, 633, 870, 953, 1106
SatI GCNGC 2 cut(s) 669, 1352
Sau3AI GATC 3 cut(s) 1239, 1328, 1332
Sau96I GGNCC 4 cut(s) 460, 590, 623, 938
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 3 cut(s) 397, 714, 715
SduI GDGCHC 2 cut(s) 931, 989
SfaNI GCATC 2 cut(s) 346, 1153
SfcI CTRYAG 1 cut(s) 80
SfuI TTCGAA 1 cut(s) 206
SinI GGWCC 3 cut(s) 460, 590, 938
SmaI CCCGGG 1 cut(s) 715
SmiMI CAYNNNNRTG 1 cut(s) 99
SmlI CTYRAG 2 cut(s) 341, 1130
SmoI CTYRAG 2 cut(s) 341, 1130
Sse9I AATT 9 cut(s) 208, 386, 423, 662, 691, 781, 804, 1020, 1261
SsiI CCGC 5 cut(s) 5, 61, 747, 912, 1288
SspMI CTAG 3 cut(s) 564, 995, 1055
StyD4I CCNGG 3 cut(s) 395, 712, 713
TaaI ACNGT 5 cut(s) 403, 464, 754, 933, 979
TaiI ACGT 1 cut(s) 371
TasI AATT 9 cut(s) 208, 386, 423, 662, 691, 781, 804, 1020, 1261
TatI WGTACW 2 cut(s) 213, 877
TfiI GAWTC 1 cut(s) 647
Tru1I TTAA 5 cut(s) 114, 633, 870, 953, 1106
Tru9I TTAA 5 cut(s) 114, 633, 870, 953, 1106
TscAI CASTG 1 cut(s) 823
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 2 cut(s) 668, 1351
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 2 cut(s) 715, 899
TspGWI ACGGA 1 cut(s) 840
TspMI CCCGGG 1 cut(s) 713
TspRI CASTG 1 cut(s) 823
VneI GTGCAC 1 cut(s) 927
VpaK11BI GGWCC 3 cut(s) 460, 590, 938
XapI RAATTY 3 cut(s) 208, 691, 804
XceI RCATGY 1 cut(s) 98
XmaI CCCGGG 1 cut(s) 713
XspI CTAG 3 cut(s) 564, 995, 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.