pycom17g00610

Regulation of nuclear pre-mRNA domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0010397
Physical Location & Seq
Reverse (-)
431596 .. 434737
3142 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1551 bp
ATGGGAAGTGCGTTCAATCCGCAGATTTTAGTGGAAAAGCTAGCCAAGCTCAATAGCTCTCAAGCAAGCATAGAGACTTTATCTCATTGGTGCATTTTCCATATGAATAAAGCAAGACAAGTTGTTGAAACATGGGCTAGGCAATTTCGTTGTGCTCCACGCGACCAGAGATTGGCTTTCTTGTATCTTGCAAATGATATTTTACAGAACAGTAGGCGAAAAGGTTCAGAATTTGTTGGTGAATTTTGGAAGGTTCTTCCAGATGCTCTTCGTGATGTAATTAAAAGTGGAGATGAATTTGAAAGAAATGCAGCACAACGATTGGTTAATATATGGGATGATAGAAAAGTTTTTGGTTCACACGGCCAGGTTCTGAAGCAAGAGTTTGTTGGACAGCACCCGGAAAATAGTAACAGAAATGGGAAGCATTCGGGTGCCAGATTGCAACCTGCTGGAACTGCATTGGACAAAATAGTTTCAGATTACCAAACTAATCATGGCCAGATGGACGAGGACACTATATTGAGCAAATGCAGGAGTGCTATTAGCTGTCTAGAGAGGGTTGATAAGGAAATTGGTGCCGATTTCAACTCAGGGAAATTTCATGGGTCTGCATTTGAGGAGGAGGTTCAGGGTCAGAATGCTATACTAAGGAATTGTATTGAACAACTAAGAGCTGTTGAATCATCAAGAGCTAATCTTGTGTCTCATCTAAGAGAGGCTCTTCAGGAGCAGGAGTACAAGCTGGATCAAGTCCGAAACCAACTTCAGGATGCAGAGTCCCAGTCAGAGCTGGCCACTAATATCTGCCATCGGTTAATGAATGGCGACAATGCACAACAGATTGCAGTCCAGAGCTCAAAGGAAGCTCATACCTCTGTAGCACCTTCCAGCTTTATGGTAGGAAGAGAACAATCAGCTCCAGTAATGTACACACCGCAGGTTGCTTTTGCTGAAAAATCCAGTCACGGGGAGGAAGATTCGCGCAAGTCTGCAGCTGCTGCAGTGGCAGCAAAGCTGACCGCATCAACATCCTCGGCCCAGATGCTCTCTTATGTCCTCTCCTCTCTGGCATCAGAGGGTGTCATCAGCAATCCCATTAACGAGTCTTCTGGTGATTATCCATCCGAAAAGAGGCTTAAGCTGGAGAATGACCAATCGTCGTCCCATTTACCCCGGAGCCCTAACCCATCTGTATCTCCTCCATTCTCGCATTCTGAATCTCACCCGCACAATGTCCCTACCACCAACCAACAACACACTCCCAGTGAGGCACCACCTCCCCCATCATCTTCTCCTCCACCACTTCCACCATTACCACCTCAACCACAGTACCAAGTGCCTCAATATGTGCCGTCTCCTGGACAATACACCTACAACGTGGCCCAACAACAGCCACCCCCAGTGCCTAGTTATCAACCAGGAGCTCCAGTAAACGGCGTTTCTCCCTTCATGACGCCGGCCAATTCTTATCAGAGTTTTCAAGGTTCAGATGGTAAACTCTACAACCAGCAATCACCGATGCCAATGACGCCGATTTCGAGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTD_bind

Protein Analysis

517

Amino Acids

56.9

Weight (kDa)

6.26

Isoelectric Point (pI)

62.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CID PF04818 9 - 120 3.5e-36 CID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 457, 931
AccB1I GGYRCC 3 cut(s) 434, 578, 1273
AccB7I CCANNNNNTGG 1 cut(s) 172
AccII CGCG 2 cut(s) 162, 985
AciI CCGC 4 cut(s) 20, 938, 1023, 1229
AclWI GGATC 1 cut(s) 756
AcoI YGGCCR 4 cut(s) 364, 499, 795, 1461
AcsI RAATTY 4 cut(s) 230, 242, 296, 599
AcuI CTGAAG 3 cut(s) 395, 710, 752
AcyI GRCGYC 2 cut(s) 1457, 1532
AfaI GTAC 3 cut(s) 740, 932, 1334
AfiI CCNNNNNNNGG 6 cut(s) 172, 769, 969, 1134, 1361, 1436
AflII CTTAAG 1 cut(s) 1139
AgsI TTSAA 7 cut(s) 16, 128, 302, 589, 665, 683, 1484
AhdI GACNNNNNGTC 1 cut(s) 1159
AjnI CCWGG 3 cut(s) 366, 1360, 1420
Alw21I GWGCWC 3 cut(s) 157, 860, 1429
Alw26I GTCTC 3 cut(s) 68, 711, 1362
AlwI GGATC 1 cut(s) 756
AlwNI CAGNNNCTG 2 cut(s) 373, 1001
AoxI GGCC 6 cut(s) 364, 499, 795, 1038, 1383, 1461
ApeKI GCWGC 5 cut(s) 311, 995, 998, 1001, 1010
ApoI RAATTY 4 cut(s) 230, 242, 296, 599
ArsI GACNNNNNNTTYG 1 cut(s) 1522
Asp700I GAANNNNTTC 1 cut(s) 223
AspLEI GCGC 1 cut(s) 987
AspS9I GGNCC 2 cut(s) 1039, 1384
AsuC2I CCSGG 2 cut(s) 401, 1177
AsuHPI GGTGA 4 cut(s) 251, 1127, 1217, 1509
AsuNHI GCTAGC 1 cut(s) 40
BaeI ACNNNNGTAYC 2 cut(s) 1316, 1349
BalI TGGCCA 2 cut(s) 501, 797
BanI GGYRCC 3 cut(s) 434, 578, 1273
BanII GRGCYC 3 cut(s) 860, 1184, 1429
BbsI GAAGAC 1 cut(s) 1101
Bbv12I GWGCWC 3 cut(s) 157, 860, 1429
BbvI GCAGC 5 cut(s) 323, 985, 988, 1007, 1022
BccI CCATC 6 cut(s) 499, 819, 1132, 1198, 1294, 1487
BceAI ACGGC 3 cut(s) 379, 1339, 1453
BciT130I CCWGG 3 cut(s) 368, 1362, 1422
BcnI CCSGG 2 cut(s) 401, 1177
BcoDI GTCTC 3 cut(s) 68, 711, 1362
BfaI CTAG 4 cut(s) 41, 138, 554, 1410
BfmI CTRYAG 3 cut(s) 879, 993, 1002
BfrI CTTAAG 1 cut(s) 1139
BfuAI ACCTGC 2 cut(s) 457, 931
BisI GCNGC 5 cut(s) 312, 996, 999, 1002, 1011
BlsI GCNGC 5 cut(s) 313, 997, 1000, 1003, 1012
Bme1390I CCNGG 5 cut(s) 368, 401, 1177, 1362, 1422
BmeRI GACNNNNNGTC 1 cut(s) 1159
BmgT120I GGNCC 2 cut(s) 1039, 1384
BmiI GGNNCC 4 cut(s) 436, 580, 1181, 1275
BmrFI CCNGG 5 cut(s) 368, 401, 1177, 1362, 1422
BmrI ACTGGG 3 cut(s) 778, 1260, 1397
BmsI GCATC 6 cut(s) 253, 763, 1034, 1035, 1082, 1512
BmtI GCTAGC 1 cut(s) 44
BmuI ACTGGG 3 cut(s) 778, 1260, 1397
BpiI GAAGAC 1 cut(s) 1101
BpmI CTGGAG 3 cut(s) 906, 1166, 1413
BpuEI CTTGAG 1 cut(s) 45
BpuMI CCSGG 2 cut(s) 401, 1177
BsaHI GRCGYC 2 cut(s) 1457, 1532
BsaJI CCNNGG 2 cut(s) 1035, 1175
BsaXI ACNNNNNCTCC 4 cut(s) 722, 752, 1416, 1446
Bsc4I CCNNNNNNNGG 6 cut(s) 172, 769, 969, 1134, 1361, 1436
Bse118I RCCGGY 1 cut(s) 1459
Bse1I ACTGG 6 cut(s) 784, 923, 963, 1266, 1403, 1430
BseBI CCWGG 3 cut(s) 368, 1362, 1422
BseDI CCNNGG 2 cut(s) 1035, 1175
BseGI GGATG 4 cut(s) 343, 778, 1031, 1124
BseLI CCNNNNNNNGG 6 cut(s) 172, 769, 969, 1134, 1361, 1436
BseMII CTCAG 1 cut(s) 606
BseNI ACTGG 6 cut(s) 784, 923, 963, 1266, 1403, 1430
BseRI GAGGAG 5 cut(s) 635, 638, 1054, 1191, 1287
BseXI GCAGC 5 cut(s) 323, 985, 988, 1007, 1022
Bsh1236I CGCG 2 cut(s) 162, 985
BshFI GGCC 6 cut(s) 366, 501, 797, 1040, 1385, 1463
BshNI GGYRCC 3 cut(s) 434, 578, 1273
BsiHKAI GWGCWC 3 cut(s) 157, 860, 1429
BsiSI CCGG 3 cut(s) 401, 1177, 1460
BslFI GGGAC 3 cut(s) 766, 1150, 1223
BslI CCNNNNNNNGG 6 cut(s) 172, 769, 969, 1134, 1361, 1436
BsmAI GTCTC 3 cut(s) 68, 711, 1362
BsmBI CGTCTC 1 cut(s) 1362
BsmFI GGGAC 3 cut(s) 766, 1150, 1223
BsmI GAATGC 3 cut(s) 427, 646, 1213
BsnI GGCC 6 cut(s) 366, 501, 797, 1040, 1385, 1463
Bsp1286I GDGCHC 4 cut(s) 157, 860, 1184, 1429
Bsp1407I TGTACA 1 cut(s) 930
Bsp143I GATC 1 cut(s) 748
BspACI CCGC 4 cut(s) 20, 938, 1023, 1229
BspANI GGCC 6 cut(s) 366, 501, 797, 1040, 1385, 1463
BspCNI CTCAG 1 cut(s) 605
BspFNI CGCG 2 cut(s) 162, 985
BspHI TCATGA 1 cut(s) 1452
BspLI GGNNCC 4 cut(s) 436, 580, 1181, 1275
BspMAI CTGCAG 2 cut(s) 997, 1006
BspMI ACCTGC 2 cut(s) 457, 931
BspOI GCTAGC 1 cut(s) 44
BspPI GGATC 1 cut(s) 756
BspQI GCTCTTC 2 cut(s) 273, 729
BspT107I GGYRCC 3 cut(s) 434, 578, 1273
BspTI CTTAAG 1 cut(s) 1139
BsrFI RCCGGY 1 cut(s) 1459
BsrGI TGTACA 1 cut(s) 930
BsrI ACTGG 6 cut(s) 784, 923, 963, 1266, 1403, 1430
BssAI RCCGGY 1 cut(s) 1459
BssECI CCNNGG 2 cut(s) 1035, 1175
BssMI GATC 1 cut(s) 748
BssNI GRCGYC 2 cut(s) 1457, 1532
Bst2UI CCWGG 3 cut(s) 368, 1362, 1422
Bst4CI ACNGT 2 cut(s) 212, 1332
Bst6I CTCTTC 3 cut(s) 273, 729, 901
BstACI GRCGYC 2 cut(s) 1457, 1532
BstAFI CTTAAG 1 cut(s) 1139
BstAPI GCANNNNNTGC 1 cut(s) 1001
BstAUI TGTACA 1 cut(s) 930
BstC8I GCNNGC 4 cut(s) 42, 67, 795, 1461
BstDEI CTNAG 4 cut(s) 592, 650, 671, 713
BstF5I GGATG 4 cut(s) 343, 778, 1031, 1124
BstFNI CGCG 2 cut(s) 162, 985
BstHHI GCGC 1 cut(s) 987
BstKTI GATC 1 cut(s) 751
BstMAI GTCTC 3 cut(s) 68, 711, 1362
BstMBI GATC 1 cut(s) 748
BstMWI GCNNNNNNNGC 6 cut(s) 46, 458, 1001, 1007, 1010, 1531
BstNI CCWGG 3 cut(s) 368, 1362, 1422
BstSCI CCNGG 5 cut(s) 366, 399, 1175, 1360, 1420
BstSFI CTRYAG 3 cut(s) 879, 993, 1002
BstUI CGCG 2 cut(s) 162, 985
BstV1I GCAGC 5 cut(s) 323, 985, 988, 1007, 1022
BstV2I GAAGAC 1 cut(s) 1101
BstXI CCANNNNNNTGG 1 cut(s) 898
BsuRI GGCC 6 cut(s) 366, 501, 797, 1040, 1385, 1463
BtsCI GGATG 4 cut(s) 343, 778, 1031, 1124
BtsI GCAGTG 1 cut(s) 1011
BtsIMutI CAGTG 3 cut(s) 1011, 1273, 1410
BveI ACCTGC 2 cut(s) 457, 931
Cac8I GCNNGC 4 cut(s) 42, 67, 795, 1461
CaiI CAGNNNCTG 2 cut(s) 373, 1001
CciI TCATGA 1 cut(s) 1452
CfoI GCGC 1 cut(s) 987
Cfr10I RCCGGY 1 cut(s) 1459
Cfr13I GGNCC 2 cut(s) 1039, 1384
CseI GACGC 2 cut(s) 1465, 1540
Csp6I GTAC 3 cut(s) 739, 931, 1333
CviAII CATG 4 cut(s) 132, 497, 605, 1453
CviQI GTAC 3 cut(s) 739, 931, 1333
DdeI CTNAG 4 cut(s) 592, 650, 671, 713
DpnI GATC 1 cut(s) 750
DpnII GATC 1 cut(s) 748
DriI GACNNNNNGTC 1 cut(s) 1159
EaeI YGGCCR 4 cut(s) 364, 499, 795, 1461
Eam1104I CTCTTC 3 cut(s) 273, 729, 901
Eam1105I GACNNNNNGTC 1 cut(s) 1159
EarI CTCTTC 3 cut(s) 273, 729, 901
Ecl136II GAGCTC 2 cut(s) 858, 1427
Eco24I GRGCYC 3 cut(s) 860, 1184, 1429
Eco53kI GAGCTC 2 cut(s) 858, 1427
Eco57I CTGAAG 3 cut(s) 395, 710, 752
EcoICRI GAGCTC 2 cut(s) 858, 1427
EcoRII CCWGG 3 cut(s) 366, 1360, 1420
EcoT38I GRGCYC 3 cut(s) 860, 1184, 1429
Esp3I CGTCTC 1 cut(s) 1362
FaeI CATG 4 cut(s) 135, 500, 608, 1456
FaqI GGGAC 3 cut(s) 766, 1150, 1223
FatI CATG 4 cut(s) 131, 496, 604, 1452
FauI CCCGC 1 cut(s) 1236
FauNDI CATATG 1 cut(s) 102
Fnu4HI GCNGC 5 cut(s) 312, 996, 999, 1002, 1011
FokI GGATG 4 cut(s) 350, 785, 1018, 1111
FriOI GRGCYC 3 cut(s) 860, 1184, 1429
Fsp4HI GCNGC 5 cut(s) 312, 996, 999, 1002, 1011
FspBI CTAG 4 cut(s) 41, 138, 554, 1410
GlaI GCGC 1 cut(s) 986
GluI GCNGC 5 cut(s) 312, 996, 999, 1002, 1011
GsuI CTGGAG 3 cut(s) 906, 1166, 1413
HaeIII GGCC 6 cut(s) 366, 501, 797, 1040, 1385, 1463
HapII CCGG 3 cut(s) 401, 1177, 1460
HgaI GACGC 2 cut(s) 1465, 1540
HhaI GCGC 1 cut(s) 987
Hin1I GRCGYC 2 cut(s) 1457, 1532
Hin1II CATG 4 cut(s) 135, 500, 608, 1456
Hin6I GCGC 1 cut(s) 985
HinP1I GCGC 1 cut(s) 985
HinfI GANTC 5 cut(s) 683, 779, 980, 1106, 1220
HpaII CCGG 3 cut(s) 401, 1177, 1460
HphI GGTGA 4 cut(s) 251, 1127, 1217, 1509
Hpy166II GTNNAC 4 cut(s) 359, 933, 1435, 1499
Hpy188III TCNNGA 8 cut(s) 260, 272, 554, 690, 728, 770, 853, 1453
Hpy8I GTNNAC 4 cut(s) 359, 933, 1435, 1499
Hpy99I CGWCG 1 cut(s) 1165
HpyAV CCTTC 3 cut(s) 244, 897, 1459
HpyCH4III ACNGT 2 cut(s) 212, 1332
HpyCH4IV ACGT 1 cut(s) 1380
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 458, 1001, 1007, 1010, 1531
HpyF3I CTNAG 4 cut(s) 592, 650, 671, 713
HpySE526I ACGT 1 cut(s) 1380
Hsp92I GRCGYC 2 cut(s) 1457, 1532
Hsp92II CATG 4 cut(s) 135, 500, 608, 1456
HspAI GCGC 1 cut(s) 985
KroI GCCGGC 1 cut(s) 1459
KroNI GCCGGC 1 cut(s) 1461
Kzo9I GATC 1 cut(s) 748
LguI GCTCTTC 2 cut(s) 273, 729
LmnI GCTCC 6 cut(s) 160, 730, 925, 1179, 1424, 1432
Lsp1109I GCAGC 5 cut(s) 323, 985, 988, 1007, 1022
LweI GCATC 6 cut(s) 253, 763, 1034, 1035, 1082, 1512
MaeI CTAG 4 cut(s) 41, 138, 554, 1410
MaeII ACGT 1 cut(s) 1380
MaeIII GTNAC 2 cut(s) 410, 965
MalI GATC 1 cut(s) 750
MboI GATC 1 cut(s) 748
MboII GAAGA 7 cut(s) 248, 260, 716, 918, 989, 1101, 1284
MhlI GDGCHC 4 cut(s) 157, 860, 1184, 1429
MlsI TGGCCA 2 cut(s) 501, 797
MluCI AATT 9 cut(s) 143, 230, 242, 279, 296, 573, 599, 655, 1465
MluNI TGGCCA 2 cut(s) 501, 797
MlyI GAGTC 2 cut(s) 788, 1115
MmeI TCCRAC 1 cut(s) 370
Mox20I TGGCCA 2 cut(s) 501, 797
MroNI GCCGGC 1 cut(s) 1459
MroXI GAANNNNTTC 1 cut(s) 223
MscI TGGCCA 2 cut(s) 501, 797
MseI TTAA 5 cut(s) 282, 327, 818, 1101, 1140
MslI CAYNNNNRTG 1 cut(s) 432
Msp20I TGGCCA 2 cut(s) 501, 797
MspA1I CMGCKG 1 cut(s) 998
MspCI CTTAAG 1 cut(s) 1139
MspI CCGG 3 cut(s) 401, 1177, 1460
MspR9I CCNGG 5 cut(s) 368, 401, 1177, 1362, 1422
Mva1269I GAATGC 3 cut(s) 427, 646, 1213
MvaI CCWGG 3 cut(s) 368, 1362, 1422
MvnI CGCG 2 cut(s) 162, 985
MwoI GCNNNNNNNGC 6 cut(s) 46, 458, 1001, 1007, 1010, 1531
NaeI GCCGGC 1 cut(s) 1461
NciI CCSGG 2 cut(s) 401, 1177
NdeI CATATG 1 cut(s) 102
NdeII GATC 1 cut(s) 748
NgoMIV GCCGGC 1 cut(s) 1459
NheI GCTAGC 1 cut(s) 40
NlaIII CATG 4 cut(s) 135, 500, 608, 1456
NlaIV GGNNCC 4 cut(s) 436, 580, 1181, 1275
NmeAIII GCCGAG 1 cut(s) 1016
NmuCI GTSAC 1 cut(s) 965
PagI TCATGA 1 cut(s) 1452
PciSI GCTCTTC 2 cut(s) 273, 729
PcsI WCGNNNNNNNCGW 1 cut(s) 1538
PctI GAATGC 3 cut(s) 427, 646, 1213
PdiI GCCGGC 1 cut(s) 1461
PdmI GAANNNNTTC 1 cut(s) 223
PfeI GAWTC 3 cut(s) 683, 980, 1220
PflMI CCANNNNNTGG 1 cut(s) 172
PfoI TCCNGGA 1 cut(s) 1360
PkrI GCNGC 5 cut(s) 313, 997, 1000, 1003, 1012
PleI GAGTC 2 cut(s) 787, 1114
PpsI GAGTC 2 cut(s) 787, 1114
Psp124BI GAGCTC 2 cut(s) 860, 1429
Psp6I CCWGG 3 cut(s) 366, 1360, 1420
PspGI CCWGG 3 cut(s) 366, 1360, 1420
PspN4I GGNNCC 4 cut(s) 436, 580, 1181, 1275
PspPI GGNCC 2 cut(s) 1039, 1384
PstI CTGCAG 2 cut(s) 997, 1006
PstNI CAGNNNCTG 2 cut(s) 373, 1001
PvuII CAGCTG 1 cut(s) 998
RsaI GTAC 3 cut(s) 740, 932, 1334
RsaNI GTAC 3 cut(s) 739, 931, 1333
RseI CAYNNNNRTG 1 cut(s) 432
SacI GAGCTC 2 cut(s) 860, 1429
SapI GCTCTTC 2 cut(s) 273, 729
SaqAI TTAA 5 cut(s) 282, 327, 818, 1101, 1140
SatI GCNGC 5 cut(s) 312, 996, 999, 1002, 1011
Sau3AI GATC 1 cut(s) 748
Sau96I GGNCC 2 cut(s) 1039, 1384
SchI GAGTC 2 cut(s) 788, 1115
ScrFI CCNGG 5 cut(s) 368, 401, 1177, 1362, 1422
SduI GDGCHC 4 cut(s) 157, 860, 1184, 1429
SfaNI GCATC 6 cut(s) 253, 763, 1034, 1035, 1082, 1512
SfcI CTRYAG 3 cut(s) 879, 993, 1002
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 2 cut(s) 60, 1139
SmoI CTYRAG 2 cut(s) 60, 1139
Sse9I AATT 9 cut(s) 143, 230, 242, 279, 296, 573, 599, 655, 1465
SsiI CCGC 4 cut(s) 20, 938, 1023, 1229
SspMI CTAG 4 cut(s) 41, 138, 554, 1410
SstI GAGCTC 2 cut(s) 860, 1429
StyD4I CCNGG 5 cut(s) 366, 399, 1175, 1360, 1420
TaaI ACNGT 2 cut(s) 212, 1332
TaiI ACGT 1 cut(s) 1383
TaqI TCGA 1 cut(s) 1541
TasI AATT 9 cut(s) 143, 230, 242, 279, 296, 573, 599, 655, 1465
TatI WGTACW 2 cut(s) 738, 930
TfiI GAWTC 3 cut(s) 683, 980, 1220
Tru1I TTAA 5 cut(s) 282, 327, 818, 1101, 1140
Tru9I TTAA 5 cut(s) 282, 327, 818, 1101, 1140
TscAI CASTG 3 cut(s) 1011, 1273, 1410
TseFI GTSAC 1 cut(s) 965
TseI GCWGC 5 cut(s) 311, 995, 998, 1001, 1010
Tsp45I GTSAC 1 cut(s) 965
TspDTI ATGAA 5 cut(s) 119, 309, 593, 836, 1441
TspRI CASTG 3 cut(s) 1011, 1273, 1410
Van91I CCANNNNNTGG 1 cut(s) 172
Vha464I CTTAAG 1 cut(s) 1139
XapI RAATTY 4 cut(s) 230, 242, 296, 599
XbaI TCTAGA 1 cut(s) 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 494
XmnI GAANNNNTTC 1 cut(s) 223
XspI CTAG 4 cut(s) 41, 138, 554, 1410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.