pycom17g03360

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0011810
Physical Location & Seq
Forward (+)
2226805 .. 2231053
4249 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2328 bp
ATGGATCTGGAGGGTGTGATCCGCAGCCATCCAATCAAGAAAGATTCATGGAAGACGGTGTTAACTCTGGCTTACCAGAGCCTGGGAGTAGTTTATGGAGATTTAAGCACTTCTCCTCTCTATGTCTACAAAAGCACTTTTGCAGAAGACATTCACCACTCAGAAACCAATGAGGAGATTTTTGGGGTTCTCTCGTTTGTGTTCTGGACGCTGACGCTGATTCCATTAGTGAAATATGTGTTTATTGTGCTTAGAGCCGACGACAATGGCGAAGGTGGGACTTTTGCTCTGTATTCTCTGCTTTCCCGGCACGCCCGGGTGAGCTCCTTGCCCAATTGCCAGCTGGCGGATGAGGAGCTGTCGGAGTACACAAAAGATGGGGTTGTTCCGACAGCGAACAGCGCTTTCGGGTCGAGTTTGAAATCGACATTGGAGAAGCATAAGGCGCTGCAAAAGGTGCTGCTTGTTCTGGCTTTGATTGGAACTTGTATGGTTATTGGTGATGGGGTGCTCACACCAGCAATTTCTGTGTTTTCCGCGGTTTCGGGGTTGGAGCTCTCCATGTCGAAAGAGCAGCATCGTTATGTTGAAGTCCCTGTTGCTTGTGTCATCCTCATATTTCTGTTTGCCCTTCAACATTACGGGACTCACCGAGTAGGGTTCTTGTTTGCACCAGTTGTGATCACATGGCTTTTGTGCATCAGTTCCATTGGTGTATACAACATCATTTACTGGAATCCCCATGTTTATCAGGCACTCTCTCCATACTACATGTACAAATTTTTTAAGAAGACTCGAAAAGGAGGTTGGATGTCCTTGGGCGGTATACTGCTGTGCATGACAGGCTCGGAAGCTATGTTTGCTGATCTTGGACACTTTTCGCAGACGTCAATCAAGATTGCTTTCACGTTTGTGGTTTACCCGTCCTTGATTCTAGCATACATGGGACAAGCTGCATACCTTTCTAAGCATCATATCATAGAAAGTGATTATCGAATTGGATTTTATGAATCAGTTCCTGAGAAATTAAGATGGCCTGTCTTGGCAATAGCCATACTTGCTGCGGTGGTGGGAAGTCAAGCAATCATAACTGGAACTTTCTCGATAATCAAACAATGTTCTGCCTTGGGTTGCTTTCCGAGGGTCAAAATTGTCCACACGTCTTCGAAAATACACGGTCAAATTTACATCCCCGAGATTAACTGGACTTTGATGTTTCTATGCTTGGCTGTTACCATTGGTTTTAGAGACACAAAGGCCATGGGAAATGCATCAGGTTTGGCCGTTATAACCGTCATGCTGGTAACTACCTGCCTTATGTCTCTAGTCATAGTCTTGTGCTGGCACAAAAGCCTCTTCTGGGCAATTTGCTTTATATTATTCTTCGGCTCCCTTGAGGCACTCTACTTCTCAGCATCGCTCATGAAGTTTCGTGAAGGGGCTTGGGTCCCGATTGCTCTTTCATTCATCTTCTTAATGGTTATGTATGTTTGGCACTACGGCACCTTCAAGAAATATGAGTTTGACGTCCAAAATAAAGTCTCCATCAACTGGCTCCTCAATTTAGGTCCTACTCTAGGGATTGTTCGAGTCCGGGGGATTGGCCTTATACACACTGAGCTCGTCTCTGGGATCCCGGCTATTTTCTCTCACTTTGTTACCAACCTCCCTGCTTTCCATCAGGTTGTAGTTTTCCTCTGCATCAAATCCGTCCCAGTCCCACATGTCAGACCCGAGGAAAGATTCTTAGTGGGAAGAGTTGGTCCAAAGGAATACCGGCTATATAGATGCATAGCACGCTATGGTTACCGTGATGTTCACAAGGATGACATGGAATTCGAAAGAGATCTGATTTGTAGTATTGCAGAGTTCATTCGATCAGAGAGACCAGAATGCAATCTGAGTCTAGTAAAATTGGAAGATGATGAGAAAATGACAGTTGTTGGGACGTCATCATCAAATTTAGACGGCATAAGAATGTCTGTAGATGAACCGGATTCCTCCGAAATAGCAAGCACCTCAGAGTTGAAGGAGATAGGGCCTACCGAAAAGGTAAAGAAGAGAGTGAGGTTCGTTGTTCCAGAAACCCCACAAATCGACAGGGAAGCAGTAGGGGAGCTGCAGGAACTGATGGAAGCAAGGGAGGCAGGAATGGCATTTATATTAGGCCACTCTTACGTGAAGGCGAAGAGAGGATCGAGATTTATGACGAAAATAGTGATCAATTTCGGGTACGATTTCTTGAGGAGGAATTTTCGAGGGCCGAGTTATGCTTTGAGCATTCCTCATGTATCTACTCTAGAGGTTGGGATGGTCTACCACGTATAA

Protein Analysis

776

Amino Acids

87.03

Weight (kDa)

8.33

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 23 - 520 6.5e-172 K+ potassium transporter integral membrane domain
K_trans_C PF22776 533 - 774 5.1e-26 K+ potassium transporter C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011810)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14880
fragaria_vesca FvH4_6g50500 FvH4_6g50500
malus_domestica MD09G1035900.v1.1 MD17G1037500.v1.1
prunus_persica Prupe.3G281900_v2.0.a1 Prupe.3G281900_v2.0.a1
pyrus_communis pycom111g02870 pycom17g03360
rosa_chinensis RchiOBHm_Chr2g0170921
rosa_laevigata RLG00000021992
rosa_multiflora Rmu_sc0009938.1_g000001
rosa_roxburghii Rroxscaffold_2G00080980
rosa_rugosa Rorug02G0552300
rosa_samantha Rh2AG625000 Rh2BG635700 Rh2CG604400 Rh2DG647300
rosa_wichuraiana Rw2G051770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1289
AatII GACGTC 3 cut(s) 890, 1530, 1952
Acc36I ACCTGC 1 cut(s) 1319
AccB1I GGYRCC 1 cut(s) 1502
AccB7I CCANNNNNTGG 2 cut(s) 82, 1551
AccI GTMKAC 4 cut(s) 126, 717, 826, 2316
AccII CGCG 1 cut(s) 539
AciI CCGC 6 cut(s) 22, 347, 537, 539, 822, 1064
AclWI GGATC 5 cut(s) 12, 13, 1627, 1640, 2203
AcoI YGGCCR 1 cut(s) 1281
AcsI RAATTY 5 cut(s) 779, 1182, 1835, 1960, 2251
AcyI GRCGYC 3 cut(s) 887, 1527, 1949
AfaI GTAC 3 cut(s) 368, 776, 2234
AfeI AGCGCT 1 cut(s) 403
AfiI CCNNNNNNNGG 5 cut(s) 82, 346, 1360, 1551, 1577
AflIII ACRYGT 3 cut(s) 771, 1158, 1723
AgsI TTSAA 5 cut(s) 421, 590, 635, 1510, 2029
AjiI CACGTC 1 cut(s) 1161
AjnI CCWGG 1 cut(s) 81
AjuI GAANNNNNNNTTGG 4 cut(s) 413, 445, 790, 822
AleI CACNNNNGTG 1 cut(s) 911
AluBI AGCT 8 cut(s) 324, 343, 358, 556, 854, 953, 1621, 2119
AluI AGCT 8 cut(s) 324, 343, 358, 556, 854, 953, 1621, 2119
Alw21I GWGCWC 4 cut(s) 326, 513, 558, 1623
Alw26I GTCTC 5 cut(s) 1242, 1326, 1546, 1630, 1879
AlwI GGATC 5 cut(s) 12, 13, 1627, 1640, 2203
AlwNI CAGNNNCTG 3 cut(s) 82, 1019, 2128
Ama87I CYCGRG 3 cut(s) 315, 1193, 1733
Aor51HI AGCGCT 1 cut(s) 403
AoxI GGCC 7 cut(s) 1034, 1257, 1281, 1603, 2039, 2167, 2261
ApeKI GCWGC 7 cut(s) 24, 448, 460, 574, 953, 1061, 2119
ApoI RAATTY 5 cut(s) 779, 1182, 1835, 1960, 2251
ArsI GACNNNNNNTTYG 2 cut(s) 1820, 1852
Asp700I GAANNNNTTC 2 cut(s) 150, 1014
AspLEI GCGC 2 cut(s) 404, 448
AspS9I GGNCC 5 cut(s) 1447, 1568, 1763, 2039, 2261
AsuC2I CCSGG 5 cut(s) 307, 316, 317, 1595, 1637
AsuHPI GGTGA 4 cut(s) 146, 331, 512, 641
AsuII TTCGAA 2 cut(s) 1166, 1839
AvaI CYCGRG 3 cut(s) 315, 1193, 1733
AvaII GGWCC 3 cut(s) 1447, 1568, 1763
BamHI GGATCC 1 cut(s) 1632
BanI GGYRCC 1 cut(s) 1502
BanII GRGCYC 3 cut(s) 326, 558, 1623
BbsI GAAGAC 4 cut(s) 59, 153, 797, 1155
Bbv12I GWGCWC 4 cut(s) 326, 513, 558, 1623
BbvI GCAGC 7 cut(s) 36, 435, 447, 586, 940, 1048, 2106
BccI CCATC 8 cut(s) 36, 371, 497, 1026, 1553, 1686, 2125, 2305
BceAI ACGGC 3 cut(s) 1268, 1516, 1984
BciT130I CCWGG 1 cut(s) 83
BclI TGATCA 2 cut(s) 681, 2220
BcnI CCSGG 5 cut(s) 307, 316, 317, 1595, 1637
BcoDI GTCTC 5 cut(s) 1242, 1326, 1546, 1630, 1879
BfaI CTAG 5 cut(s) 935, 1325, 1577, 1907, 2300
BfmI CTRYAG 2 cut(s) 1983, 2120
BfoI RGCGCY 2 cut(s) 405, 449
BfuAI ACCTGC 1 cut(s) 1319
BglII AGATCT 1 cut(s) 1846
BisI GCNGC 7 cut(s) 25, 449, 461, 575, 954, 1062, 2120
BlsI GCNGC 7 cut(s) 26, 450, 462, 576, 955, 1063, 2121
Bme1390I CCNGG 6 cut(s) 83, 307, 316, 317, 1595, 1637
Bme18I GGWCC 3 cut(s) 1447, 1568, 1763
BmeT110I CYCGRG 3 cut(s) 315, 1193, 1733
BmgBI CACGTC 1 cut(s) 1161
BmgT120I GGNCC 5 cut(s) 1447, 1568, 1763, 2039, 2261
BmiI GGNNCC 6 cut(s) 1390, 1448, 1449, 1504, 1556, 1634
BmrFI CCNGG 6 cut(s) 83, 307, 316, 317, 1595, 1637
BmrI ACTGGG 1 cut(s) 1709
BmsI GCATC 7 cut(s) 586, 708, 979, 1280, 1424, 1710, 1778
BmuI ACTGGG 1 cut(s) 1709
BpiI GAAGAC 4 cut(s) 59, 153, 797, 1155
BplI GAGNNNNNCTC 4 cut(s) 1610, 1642, 2269, 2301
BpmI CTGGAG 1 cut(s) 29
Bpu14I TTCGAA 2 cut(s) 1166, 1839
BpuEI CTTGAG 2 cut(s) 1415, 2263
BpuMI CCSGG 5 cut(s) 307, 316, 317, 1595, 1637
BsaAI YACGTR 2 cut(s) 2179, 2323
BsaHI GRCGYC 3 cut(s) 887, 1527, 1949
BsaI GGTCTC 1 cut(s) 1879
BsaJI CCNNGG 9 cut(s) 82, 315, 537, 816, 1125, 1139, 1260, 1594, 1734
BsaWI WCCGGW 1 cut(s) 1993
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 346, 1360, 1551, 1577
Bse118I RCCGGY 1 cut(s) 1776
Bse1I ACTGG 6 cut(s) 674, 737, 1096, 1208, 1556, 1715
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 9 cut(s) 82, 315, 537, 816, 1125, 1139, 1260, 1594, 1734
BseGI GGATG 7 cut(s) 28, 355, 609, 816, 1188, 1831, 2316
BseLI CCNNNNNNNGG 5 cut(s) 82, 346, 1360, 1551, 1577
BseMII CTCAG 6 cut(s) 174, 1011, 1425, 1608, 1892, 2034
BseNI ACTGG 6 cut(s) 674, 737, 1096, 1208, 1556, 1715
BseRI GAGGAG 5 cut(s) 105, 188, 368, 1547, 2260
BseXI GCAGC 7 cut(s) 36, 435, 447, 586, 940, 1048, 2106
Bsh1236I CGCG 1 cut(s) 539
BshFI GGCC 7 cut(s) 1036, 1259, 1283, 1605, 2041, 2169, 2263
BshNI GGYRCC 1 cut(s) 1502
BsiHKAI GWGCWC 4 cut(s) 326, 513, 558, 1623
BsiHKCI CYCGRG 3 cut(s) 315, 1193, 1733
BsiSI CCGG 6 cut(s) 307, 316, 1594, 1637, 1777, 1994
BslFI GGGAC 8 cut(s) 292, 578, 658, 960, 1433, 1697, 1703, 1960
BslI CCNNNNNNNGG 5 cut(s) 82, 346, 1360, 1551, 1577
BsmAI GTCTC 5 cut(s) 1242, 1326, 1546, 1630, 1879
BsmBI CGTCTC 1 cut(s) 1630
BsmFI GGGAC 8 cut(s) 292, 578, 658, 960, 1433, 1697, 1703, 1960
BsmI GAATGC 2 cut(s) 1898, 2280
BsnI GGCC 7 cut(s) 1036, 1259, 1283, 1605, 2041, 2169, 2263
Bso31I GGTCTC 1 cut(s) 1879
BsoBI CYCGRG 3 cut(s) 315, 1193, 1733
Bsp119I TTCGAA 2 cut(s) 1166, 1839
Bsp1286I GDGCHC 4 cut(s) 326, 513, 558, 1623
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 9 cut(s) 4, 18, 681, 865, 1632, 1846, 1877, 2195, 2220
Bsp19I CCATGG 1 cut(s) 1260
BspACI CCGC 6 cut(s) 22, 347, 537, 539, 822, 1064
BspANI GGCC 7 cut(s) 1036, 1259, 1283, 1605, 2041, 2169, 2263
BspCNI CTCAG 6 cut(s) 173, 1012, 1424, 1609, 1893, 2033
BspFNI CGCG 1 cut(s) 539
BspHI TCATGA 1 cut(s) 1422
BspLI GGNNCC 6 cut(s) 1390, 1448, 1449, 1504, 1556, 1634
BspMAI CTGCAG 1 cut(s) 2124
BspMI ACCTGC 1 cut(s) 1319
BspPI GGATC 5 cut(s) 12, 13, 1627, 1640, 2203
BspT104I TTCGAA 2 cut(s) 1166, 1839
BspT107I GGYRCC 1 cut(s) 1502
BspTNI GGTCTC 1 cut(s) 1879
BsrFI RCCGGY 1 cut(s) 1776
BsrGI TGTACA 1 cut(s) 774
BsrI ACTGG 6 cut(s) 674, 737, 1096, 1208, 1556, 1715
BssAI RCCGGY 1 cut(s) 1776
BssECI CCNNGG 9 cut(s) 82, 315, 537, 816, 1125, 1139, 1260, 1594, 1734
BssMI GATC 9 cut(s) 4, 18, 681, 865, 1632, 1846, 1877, 2195, 2220
BssNAI GTATAC 2 cut(s) 718, 827
BssNI GRCGYC 3 cut(s) 887, 1527, 1949
BssT1I CCWWGG 3 cut(s) 816, 1125, 1260
Bst1107I GTATAC 2 cut(s) 718, 827
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 5 cut(s) 58, 1178, 1294, 1811, 1939
Bst6I CTCTTC 4 cut(s) 1361, 1750, 2054, 2183
BstACI GRCGYC 3 cut(s) 887, 1527, 1949
BstAPI GCANNNNNTGC 1 cut(s) 457
BstAUI TGTACA 1 cut(s) 774
BstBAI YACGTR 2 cut(s) 2179, 2323
BstBI TTCGAA 2 cut(s) 1166, 1839
BstC8I GCNNGC 6 cut(s) 312, 341, 345, 1343, 1798, 2014
BstDEI CTNAG 9 cut(s) 160, 251, 966, 1020, 1411, 1617, 1747, 1901, 2020
BstDSI CCRYGG 2 cut(s) 537, 1260
BstEII GGTNACC 1 cut(s) 1805
BstENI CCTNNNNNAGG 1 cut(s) 1575
BstF5I GGATG 7 cut(s) 28, 355, 609, 816, 1188, 1831, 2316
BstFNI CGCG 1 cut(s) 539
BstH2I RGCGCY 2 cut(s) 405, 449
BstHHI GCGC 2 cut(s) 404, 448
BstKTI GATC 9 cut(s) 7, 21, 684, 868, 1635, 1849, 1880, 2198, 2223
BstMAI GTCTC 5 cut(s) 1242, 1326, 1546, 1630, 1879
BstMBI GATC 9 cut(s) 4, 18, 681, 865, 1632, 1846, 1877, 2195, 2220
BstNI CCWGG 1 cut(s) 83
BstNSI RCATGY 2 cut(s) 775, 1727
BstPI GGTNACC 1 cut(s) 1805
BstSCI CCNGG 6 cut(s) 81, 305, 314, 315, 1593, 1635
BstSFI CTRYAG 2 cut(s) 1983, 2120
BstUI CGCG 1 cut(s) 539
BstV1I GCAGC 7 cut(s) 36, 435, 447, 586, 940, 1048, 2106
BstV2I GAAGAC 4 cut(s) 59, 153, 797, 1155
BstX2I RGATCY 3 cut(s) 4, 1632, 1846
BstYI RGATCY 3 cut(s) 4, 1632, 1846
BstZ17I GTATAC 2 cut(s) 718, 827
BsuRI GGCC 7 cut(s) 1036, 1259, 1283, 1605, 2041, 2169, 2263
BtgI CCRYGG 2 cut(s) 537, 1260
BtgZI GCGATG 1 cut(s) 1401
BtrI CACGTC 1 cut(s) 1161
BtsCI GGATG 7 cut(s) 28, 355, 609, 816, 1188, 1831, 2316
BtsIMutI CAGTG 1 cut(s) 1614
BveI ACCTGC 1 cut(s) 1319
Cac8I GCNNGC 6 cut(s) 312, 341, 345, 1343, 1798, 2014
CaiI CAGNNNCTG 3 cut(s) 82, 1019, 2128
CciI TCATGA 1 cut(s) 1422
CfoI GCGC 2 cut(s) 404, 448
Cfr10I RCCGGY 1 cut(s) 1776
Cfr13I GGNCC 5 cut(s) 1447, 1568, 1763, 2039, 2261
Cfr42I CCGCGG 1 cut(s) 540
Cfr9I CCCGGG 1 cut(s) 315
CseI GACGC 2 cut(s) 217, 223
Csp6I GTAC 3 cut(s) 367, 775, 2233
CviQI GTAC 3 cut(s) 367, 775, 2233
DdeI CTNAG 9 cut(s) 160, 251, 966, 1020, 1411, 1617, 1747, 1901, 2020
DpnI GATC 9 cut(s) 6, 20, 683, 867, 1634, 1848, 1879, 2197, 2222
DpnII GATC 9 cut(s) 4, 18, 681, 865, 1632, 1846, 1877, 2195, 2220
EaeI YGGCCR 1 cut(s) 1281
Eam1104I CTCTTC 4 cut(s) 1361, 1750, 2054, 2183
EarI CTCTTC 4 cut(s) 1361, 1750, 2054, 2183
EciI GGCGGA 1 cut(s) 362
Ecl136II GAGCTC 3 cut(s) 324, 556, 1621
Eco130I CCWWGG 3 cut(s) 816, 1125, 1260
Eco24I GRGCYC 3 cut(s) 326, 558, 1623
Eco31I GGTCTC 1 cut(s) 1879
Eco47I GGWCC 3 cut(s) 1447, 1568, 1763
Eco47III AGCGCT 1 cut(s) 403
Eco53kI GAGCTC 3 cut(s) 324, 556, 1621
Eco88I CYCGRG 3 cut(s) 315, 1193, 1733
Eco91I GGTNACC 1 cut(s) 1805
EcoICRI GAGCTC 3 cut(s) 324, 556, 1621
EcoNI CCTNNNNNAGG 1 cut(s) 1575
EcoO109I RGGNCCY 3 cut(s) 1447, 1568, 2039
EcoO65I GGTNACC 1 cut(s) 1805
EcoRI GAATTC 1 cut(s) 1835
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 3 cut(s) 816, 1125, 1260
EcoT22I ATGCAT 2 cut(s) 1273, 1793
EcoT38I GRGCYC 3 cut(s) 326, 558, 1623
ErhI CCWWGG 3 cut(s) 816, 1125, 1260
Esp3I CGTCTC 1 cut(s) 1630
FaqI GGGAC 8 cut(s) 292, 578, 658, 960, 1433, 1697, 1703, 1960
FbaI TGATCA 2 cut(s) 681, 2220
FblI GTMKAC 4 cut(s) 126, 717, 826, 2316
Fnu4HI GCNGC 7 cut(s) 25, 449, 461, 575, 954, 1062, 2120
FokI GGATG 7 cut(s) 15, 362, 596, 823, 1175, 1838, 2323
FriOI GRGCYC 3 cut(s) 326, 558, 1623
Fsp4HI GCNGC 7 cut(s) 25, 449, 461, 575, 954, 1062, 2120
FspBI CTAG 5 cut(s) 935, 1325, 1577, 1907, 2300
GlaI GCGC 2 cut(s) 403, 447
GluI GCNGC 7 cut(s) 25, 449, 461, 575, 954, 1062, 2120
GsuI CTGGAG 1 cut(s) 29
HaeII RGCGCY 2 cut(s) 405, 449
HaeIII GGCC 7 cut(s) 1036, 1259, 1283, 1605, 2041, 2169, 2263
HapII CCGG 6 cut(s) 307, 316, 1594, 1637, 1777, 1994
HgaI GACGC 2 cut(s) 217, 223
HhaI GCGC 2 cut(s) 404, 448
Hin1I GRCGYC 3 cut(s) 887, 1527, 1949
Hin6I GCGC 2 cut(s) 402, 446
HinP1I GCGC 2 cut(s) 402, 446
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HpaI GTTAAC 1 cut(s) 63
HpaII CCGG 6 cut(s) 307, 316, 1594, 1637, 1777, 1994
HphI GGTGA 4 cut(s) 146, 331, 512, 641
Hpy166II GTNNAC 9 cut(s) 63, 127, 369, 718, 827, 919, 1156, 1819, 2317
Hpy8I GTNNAC 9 cut(s) 63, 127, 369, 718, 827, 919, 1156, 1819, 2317
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 6 cut(s) 266, 641, 1430, 1516, 2023, 2176
HpyCH4III ACNGT 5 cut(s) 58, 1178, 1294, 1811, 1939
HpyCH4IV ACGT 7 cut(s) 887, 908, 1160, 1527, 1949, 2178, 2322
HpyF3I CTNAG 9 cut(s) 160, 251, 966, 1020, 1411, 1617, 1747, 1901, 2020
HpySE526I ACGT 7 cut(s) 887, 908, 1160, 1527, 1949, 2178, 2322
Hsp92I GRCGYC 3 cut(s) 887, 1527, 1949
HspAI GCGC 2 cut(s) 402, 446
KflI GGGWCCC 1 cut(s) 1447
Ksp22I TGATCA 2 cut(s) 681, 2220
KspAI GTTAAC 1 cut(s) 63
KspI CCGCGG 1 cut(s) 540
Kzo9I GATC 9 cut(s) 4, 18, 681, 865, 1632, 1846, 1877, 2195, 2220
LmnI GCTCC 6 cut(s) 329, 355, 553, 1394, 1560, 2116
Lsp1109I GCAGC 7 cut(s) 36, 435, 447, 586, 940, 1048, 2106
LweI GCATC 7 cut(s) 586, 708, 979, 1280, 1424, 1710, 1778
MaeI CTAG 5 cut(s) 935, 1325, 1577, 1907, 2300
MaeII ACGT 7 cut(s) 887, 908, 1160, 1527, 1949, 2178, 2322
MaeIII GTNAC 4 cut(s) 1231, 1303, 1657, 1805
MalI GATC 9 cut(s) 6, 20, 683, 867, 1634, 1848, 1879, 2197, 2222
MboI GATC 9 cut(s) 4, 18, 681, 865, 1632, 1846, 1877, 2195, 2220
MfeI CAATTG 1 cut(s) 334
MflI RGATCY 3 cut(s) 4, 1632, 1846
MhlI GDGCHC 4 cut(s) 326, 513, 558, 1623
MlyI GAGTC 4 cut(s) 640, 787, 1599, 1912
MmeI TCCRAC 4 cut(s) 342, 413, 531, 788
Mph1103I ATGCAT 2 cut(s) 1273, 1793
MroXI GAANNNNTTC 2 cut(s) 150, 1014
MseI TTAA 6 cut(s) 62, 104, 786, 1028, 1200, 1475
MslI CAYNNNNRTG 4 cut(s) 582, 911, 1824, 1976
MspA1I CMGCKG 2 cut(s) 343, 539
MspI CCGG 6 cut(s) 307, 316, 1594, 1637, 1777, 1994
MspR9I CCNGG 6 cut(s) 83, 307, 316, 317, 1595, 1637
MunI CAATTG 1 cut(s) 334
Mva1269I GAATGC 2 cut(s) 1898, 2280
MvaI CCWGG 1 cut(s) 83
MvnI CGCG 1 cut(s) 539
NciI CCSGG 5 cut(s) 307, 316, 317, 1595, 1637
NcoI CCATGG 1 cut(s) 1260
NdeII GATC 9 cut(s) 4, 18, 681, 865, 1632, 1846, 1877, 2195, 2220
NlaIV GGNNCC 6 cut(s) 1390, 1448, 1449, 1504, 1556, 1634
NmeAIII GCCGAG 1 cut(s) 2289
NsiI ATGCAT 2 cut(s) 1273, 1793
NspI RCATGY 2 cut(s) 775, 1727
NspV TTCGAA 2 cut(s) 1166, 1839
OliI CACNNNNGTG 1 cut(s) 911
PagI TCATGA 1 cut(s) 1422
PciI ACATGT 2 cut(s) 771, 1723
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 2 cut(s) 1898, 2280
PdmI GAANNNNTTC 2 cut(s) 150, 1014
PfeI GAWTC 7 cut(s) 44, 220, 736, 931, 1010, 1743, 1997
PflMI CCANNNNNTGG 2 cut(s) 82, 1551
PkrI GCNGC 7 cut(s) 26, 450, 462, 576, 955, 1063, 2121
PleI GAGTC 4 cut(s) 640, 787, 1598, 1911
PpsI GAGTC 4 cut(s) 640, 787, 1598, 1911
Ppu21I YACGTR 2 cut(s) 2179, 2323
PpuMI RGGWCCY 2 cut(s) 1447, 1568
PscI ACATGT 2 cut(s) 771, 1723
PsiI TTATAA 1 cut(s) 1289
Psp124BI GAGCTC 3 cut(s) 326, 558, 1623
Psp5II RGGWCCY 2 cut(s) 1447, 1568
Psp6I CCWGG 1 cut(s) 81
PspEI GGTNACC 1 cut(s) 1805
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 6 cut(s) 1390, 1448, 1449, 1504, 1556, 1634
PspPI GGNCC 5 cut(s) 1447, 1568, 1763, 2039, 2261
PspPPI RGGWCCY 2 cut(s) 1447, 1568
PstI CTGCAG 1 cut(s) 2124
PstNI CAGNNNCTG 3 cut(s) 82, 1019, 2128
PsuI RGATCY 3 cut(s) 4, 1632, 1846
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 3 cut(s) 368, 776, 2234
RsaNI GTAC 3 cut(s) 367, 775, 2233
RseI CAYNNNNRTG 4 cut(s) 582, 911, 1824, 1976
SacI GAGCTC 3 cut(s) 326, 558, 1623
SacII CCGCGG 1 cut(s) 540
SaqAI TTAA 6 cut(s) 62, 104, 786, 1028, 1200, 1475
SatI GCNGC 7 cut(s) 25, 449, 461, 575, 954, 1062, 2120
Sau3AI GATC 9 cut(s) 4, 18, 681, 865, 1632, 1846, 1877, 2195, 2220
Sau96I GGNCC 5 cut(s) 1447, 1568, 1763, 2039, 2261
SchI GAGTC 4 cut(s) 640, 787, 1599, 1912
ScrFI CCNGG 6 cut(s) 83, 307, 316, 317, 1595, 1637
SduI GDGCHC 4 cut(s) 326, 513, 558, 1623
SfaNI GCATC 7 cut(s) 586, 708, 979, 1280, 1424, 1710, 1778
SfcI CTRYAG 2 cut(s) 1983, 2120
Sfr303I CCGCGG 1 cut(s) 540
SfuI TTCGAA 2 cut(s) 1166, 1839
SgrBI CCGCGG 1 cut(s) 540
SinI GGWCC 3 cut(s) 1447, 1568, 1763
SmaI CCCGGG 1 cut(s) 317
SmiMI CAYNNNNRTG 4 cut(s) 582, 911, 1824, 1976
SmlI CTYRAG 2 cut(s) 1394, 2242
SmoI CTYRAG 2 cut(s) 1394, 2242
SsiI CCGC 6 cut(s) 22, 347, 537, 539, 822, 1064
SspMI CTAG 5 cut(s) 935, 1325, 1577, 1907, 2300
SstI GAGCTC 3 cut(s) 326, 558, 1623
StyD4I CCNGG 6 cut(s) 81, 305, 314, 315, 1593, 1635
StyI CCWWGG 3 cut(s) 816, 1125, 1260
TaaI ACNGT 5 cut(s) 58, 1178, 1294, 1811, 1939
TaiI ACGT 7 cut(s) 890, 911, 1163, 1530, 1952, 2181, 2325
TatI WGTACW 2 cut(s) 366, 774
TfiI GAWTC 7 cut(s) 44, 220, 736, 931, 1010, 1743, 1997
Tru1I TTAA 6 cut(s) 62, 104, 786, 1028, 1200, 1475
Tru9I TTAA 6 cut(s) 62, 104, 786, 1028, 1200, 1475
TscAI CASTG 1 cut(s) 1621
TseI GCWGC 7 cut(s) 24, 448, 460, 574, 953, 1061, 2119
TspDTI ATGAA 7 cut(s) 36, 1023, 1439, 1452, 1456, 1861, 2004
TspGWI ACGGA 1 cut(s) 1699
TspMI CCCGGG 1 cut(s) 315
TspRI CASTG 1 cut(s) 1621
Van91I CCANNNNNTGG 2 cut(s) 82, 1551
VpaK11BI GGWCC 3 cut(s) 1447, 1568, 1763
XagI CCTNNNNNAGG 1 cut(s) 1575
XapI RAATTY 5 cut(s) 779, 1182, 1835, 1960, 2251
XbaI TCTAGA 1 cut(s) 2299
XceI RCATGY 2 cut(s) 775, 1727
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XmaI CCCGGG 1 cut(s) 315
XmiI GTMKAC 4 cut(s) 126, 717, 826, 2316
XmnI GAANNNNTTC 2 cut(s) 150, 1014
XspI CTAG 5 cut(s) 935, 1325, 1577, 1907, 2300
ZraI GACGTC 3 cut(s) 888, 1528, 1950
Zsp2I ATGCAT 2 cut(s) 1273, 1793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.