pycom17g10680

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008589
Physical Location & Seq
Forward (+)
8263046 .. 8264635
1590 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1590 bp
ATGTACAAATTCCAAATTCGTCAGTTTTCGGGTTTGCCCAATTCGATTCCAAAAGAACCCACCTTTACAAACCCCACAATTTCTGGGAGTTTTGGGAAAAGGAATCGGAATCCGATCCCTATACCCCACAGAACGATTCCTGAACCCAAAGGGCAGGACCTCGATTTTGTCAATGTTGTAAGCAGCCATCTGATTCACTCGGATTGGGCTAAGCTTAATCCCTTATCGACCGGTCTAACCGCTTTTAGAGTTAAACATATATTGCTGAAAATTCAGAAGGATTATGTTCTTTCCCTTGAGTTTTTCAATTGGGTTGCTGCTCGAAACCCGAATGCACATACCCTCGAAACACATTCCATGATTCTCCACATTCTCACCAAGTACCGGAAGTTCAAATCCGCGGAGTACATTTCGAAGAAGATTCTTGTTTCGGGCTCAATTGATTTGCCTTCCAAGTTGTTTGAGGTGATTCTGTATTCGTATCGGTTGTGTGACTCCTCTCCTCGTGTCTTTGATTCGCTGTTCAAGACTTTCGCGCATTTGAAGAAGTTTAGGAATGCCACAAACATGTTCTGCCAGATGAAGGAGTATGGTTTTTTCCCAACGGTTGAGTCTTGCAATGCGTATTTGAGCTCACTGCTAGGTTTGCATAGAGCGGACGTTGCTTTGGCATTCTATAGAGAAATGCGGCGTTGTAGGATTTCGCCCAATGTTTATACTCTTAATATGGTTATGGCTGCTTACTGTAAATCGGGAAAATTAGAGAGCGCTGTTGAGGTGCTCGAGGAGATGGAGAGCATGGGGTGTAGTCCTAGTGTGGTGTCGTATAATACACTGATTGCGGGACACTGTGATAAGGGTCTTATGAGTTCGGCTTTGAAGTTCAAAAACTTGATGGCAAAGAATGGATTGCACCCTACTGTGGTAACTTTTAACACACTTATTGATGGGTTTTGCAAGGCAGGAAAACTACAAGAAGCAAATAGAGTTTTTAGCGAGATGAAGGCCGCGAATGTGGCTCCTAATACAGTAACTTACAATAGTTTGATAAACGGGTACAGCCAGGCAGGTAATAGTGAGATGGGCACTAGGCTTTTCGAGGAGATGTCAAAGAACCGAGTAAATGCTGATATACTGACTTATAATGCACTGATATTGGGACTCTGCAAGGAGGGTAAAACAAAGAAAGCTGCATATCTGGTTAAAGAACTTGATGCAAAGCACTTTGTCCCGAATGCCTCAACCTTTTCTGCCCTTATTGTAGGACAGTGTGTCCGAAAGAATGCTGATCGTGCCTTTCAGTTATGCCAAAGCATGATAAGAAGTGGTCATCATCCTGATGAACAAACTTTGAAGACGCTTATATCAAGCTTCTGCGGTAACAGAGATTTTGAGGGAGCAGTCCAAGTCTTGGAAGAAATGTTAGAGAGATCTATTGCCCTTGATTCAGGTATCTTATCTGATTTATGTCTAGGACTTCACCGATGTGGGAAAGAAGAATTGGTAAAGTCAGTATGCGGTAAGTTGGAAGCTAGACGACTCATGCCTCAAGGTTTTGATATGGCGAAGAAATTATCTACTCTGGATTAG

Protein Analysis

530

Amino Acids

59.31

Weight (kDa)

9.24

Isoelectric Point (pI)

31.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 148 - 281 4.7e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 201 - 244 5.5e-06 PPR repeat family
PPR_3 PF13812 227 - 280 1.5e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 234 - 265 3.9e-09 PPR repeat
PPR_2 PF13041 236 - 280 3.3e-14 PPR repeat family
PPR PF01535 240 - 269 2.7e-07 PPR repeat
PPR_1 PF12854 267 - 299 4.7e-09 PPR repeat
PPR_2 PF13041 271 - 320 2.3e-17 PPR repeat family
PPR_3 PF13812 299 - 353 1.5e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 302 - 335 1.5e-15 PPR repeat
PPR_2 PF13041 306 - 353 1.7e-19 PPR repeat family
PPR_long PF17177 307 - 419 4.5e-08 Pentacotripeptide-repeat region of PRORP
PPR PF01535 309 - 338 3.4e-09 PPR repeat
TPR_24 PF23276 310 - 400 1.6e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 338 - 370 6.7e-08 PPR repeat
PPR_2 PF13041 341 - 390 1.3e-15 PPR repeat family
PPR PF01535 344 - 373 4.6e-08 PPR repeat
PPR_3 PF13812 364 - 420 2.4e-07 Pentatricopeptide repeat domain
PPR_long PF17177 365 - 502 5.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 374 - 403 2e-06 PPR repeat
PPR_2 PF13041 377 - 424 1.9e-09 PPR repeat family
PPR_2 PF13041 411 - 459 8.7e-08 PPR repeat family
PPR_1 PF12854 443 - 474 9.2e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1143
Acc36I ACCTGC 1 cut(s) 1058
AccB7I CCANNNNNTGG 1 cut(s) 1411
AccBSI CCGCTC 1 cut(s) 656
AccII CGCG 3 cut(s) 401, 536, 1010
AciI CCGC 9 cut(s) 240, 399, 401, 656, 688, 842, 1008, 1377, 1518
AclWI GGATC 1 cut(s) 109
AcsI RAATTY 3 cut(s) 8, 15, 270
AfaI GTAC 4 cut(s) 5, 383, 407, 1058
AfeI AGCGCT 1 cut(s) 769
AfiI CCNNNNNNNGG 4 cut(s) 384, 583, 922, 1411
AflIII ACRYGT 1 cut(s) 567
AgeI ACCGGT 1 cut(s) 230
AgsI TTSAA 7 cut(s) 307, 394, 526, 544, 880, 886, 1354
AhdI GACNNNNNGTC 1 cut(s) 1271
AjnI CCWGG 1 cut(s) 1062
AjuI GAANNNNNNNTTGG 2 cut(s) 1484, 1516
AleI CACNNNNGTG 1 cut(s) 1485
AluBI AGCT 5 cut(s) 214, 633, 1190, 1371, 1532
AluI AGCT 5 cut(s) 214, 633, 1190, 1371, 1532
Alw21I GWGCWC 2 cut(s) 635, 783
AlwI GGATC 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 782
Aor51HI AGCGCT 1 cut(s) 769
AoxI GGCC 1 cut(s) 1005
ApeKI GCWGC 4 cut(s) 183, 317, 737, 1190
ApoI RAATTY 3 cut(s) 8, 15, 270
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AsiGI ACCGGT 1 cut(s) 230
AspLEI GCGC 2 cut(s) 538, 770
AspS9I GGNCC 1 cut(s) 157
AsuHPI GGTGA 3 cut(s) 367, 478, 1472
AsuII TTCGAA 1 cut(s) 413
AvaI CYCGRG 1 cut(s) 782
AvaII GGWCC 1 cut(s) 157
BaeGI GKGCMC 1 cut(s) 1088
BanII GRGCYC 2 cut(s) 437, 635
BauI CACGAG 1 cut(s) 504
BbsI GAAGAC 1 cut(s) 1361
Bbv12I GWGCWC 2 cut(s) 635, 783
BbvI GCAGC 4 cut(s) 195, 304, 724, 1177
BccI CCATC 5 cut(s) 195, 784, 889, 941, 1075
BciT130I CCWGG 1 cut(s) 1064
BfaI CTAG 5 cut(s) 641, 813, 1089, 1472, 1533
BfmI CTRYAG 1 cut(s) 676
BfoI RGCGCY 1 cut(s) 771
BfuAI ACCTGC 1 cut(s) 1058
BglII AGATCT 1 cut(s) 1430
BisI GCNGC 6 cut(s) 184, 318, 689, 738, 1008, 1191
BlpI GCTNAGC 1 cut(s) 210
BlsI GCNGC 6 cut(s) 185, 319, 690, 739, 1009, 1192
Bme1390I CCNGG 1 cut(s) 1064
Bme18I GGWCC 1 cut(s) 157
BmeRI GACNNNNNGTC 1 cut(s) 1271
BmeT110I CYCGRG 1 cut(s) 782
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 1 cut(s) 1020
BmrFI CCNGG 1 cut(s) 1064
BmsI GCATC 1 cut(s) 1204
BpiI GAAGAC 1 cut(s) 1361
Bpu1102I GCTNAGC 1 cut(s) 210
Bpu14I TTCGAA 1 cut(s) 413
BpuEI CTTGAG 2 cut(s) 317, 1533
BsaJI CCNNGG 1 cut(s) 399
BsaWI WCCGGW 2 cut(s) 230, 384
Bsc4I CCNNNNNNNGG 4 cut(s) 384, 583, 922, 1411
Bse118I RCCGGY 1 cut(s) 230
Bse3DI GCAATG 1 cut(s) 625
BseBI CCWGG 1 cut(s) 1064
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 1 cut(s) 1333
BseLI CCNNNNNNNGG 4 cut(s) 384, 583, 922, 1411
BseMI GCAATG 1 cut(s) 625
BseRI GAGGAG 4 cut(s) 487, 492, 800, 1115
BseSI GKGCMC 1 cut(s) 1088
BseXI GCAGC 4 cut(s) 195, 304, 724, 1177
Bsh1236I CGCG 3 cut(s) 401, 536, 1010
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 1 cut(s) 1007
BshTI ACCGGT 1 cut(s) 230
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 2 cut(s) 635, 783
BsiHKCI CYCGRG 1 cut(s) 782
BsiSI CCGG 2 cut(s) 231, 385
BslFI GGGAC 3 cut(s) 858, 1173, 1214
BslI CCNNNNNNNGG 4 cut(s) 384, 583, 922, 1411
BsmFI GGGAC 3 cut(s) 858, 1173, 1214
BsmI GAATGC 5 cut(s) 337, 562, 671, 1240, 1288
BsnI GGCC 1 cut(s) 1007
BsoBI CYCGRG 1 cut(s) 782
Bsp119I TTCGAA 1 cut(s) 413
Bsp1286I GDGCHC 4 cut(s) 437, 635, 783, 1088
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 114, 1288, 1430
Bsp1720I GCTNAGC 1 cut(s) 210
BspACI CCGC 9 cut(s) 240, 399, 401, 656, 688, 842, 1008, 1377, 1518
BspANI GGCC 1 cut(s) 1007
BspFNI CGCG 3 cut(s) 401, 536, 1010
BspLI GGNNCC 1 cut(s) 1020
BspMI ACCTGC 1 cut(s) 1058
BspPI GGATC 1 cut(s) 109
BspT104I TTCGAA 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 656
BsrDI GCAATG 1 cut(s) 625
BsrFI RCCGGY 1 cut(s) 230
BsrGI TGTACA 1 cut(s) 3
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 3 cut(s) 114, 1288, 1430
BssSI CACGAG 1 cut(s) 504
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 1 cut(s) 1064
Bst4CI ACNGT 6 cut(s) 607, 746, 851, 922, 1030, 1269
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 413
BstDEI CTNAG 1 cut(s) 210
BstDSI CCRYGG 1 cut(s) 399
BstF5I GGATG 1 cut(s) 1333
BstFNI CGCG 3 cut(s) 401, 536, 1010
BstH2I RGCGCY 1 cut(s) 771
BstHHI GCGC 2 cut(s) 538, 770
BstKTI GATC 3 cut(s) 117, 1291, 1433
BstMBI GATC 3 cut(s) 114, 1288, 1430
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 4 cut(s) 646, 662, 1016, 1292
BstNI CCWGG 1 cut(s) 1064
BstNSI RCATGY 1 cut(s) 571
BstSCI CCNGG 1 cut(s) 1062
BstSFI CTRYAG 1 cut(s) 676
BstSLI GKGCMC 1 cut(s) 1088
BstUI CGCG 3 cut(s) 401, 536, 1010
BstV1I GCAGC 4 cut(s) 195, 304, 724, 1177
BstV2I GAAGAC 1 cut(s) 1361
BstX2I RGATCY 1 cut(s) 1430
BstYI RGATCY 1 cut(s) 1430
BsuRI GGCC 1 cut(s) 1007
BtgI CCRYGG 1 cut(s) 399
BtsCI GGATG 1 cut(s) 1333
BtsI GCAGTG 1 cut(s) 635
BtsIMutI CAGTG 5 cut(s) 635, 833, 847, 1148, 1274
BveI ACCTGC 1 cut(s) 1058
CfoI GCGC 2 cut(s) 538, 770
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 157
Cfr42I CCGCGG 1 cut(s) 402
CseI GACGC 1 cut(s) 1366
Csp6I GTAC 4 cut(s) 4, 382, 406, 1057
CspAI ACCGGT 1 cut(s) 230
CviAII CATG 5 cut(s) 358, 568, 799, 1315, 1543
CviQI GTAC 4 cut(s) 4, 382, 406, 1057
DdeI CTNAG 1 cut(s) 210
DpnI GATC 3 cut(s) 116, 1290, 1432
DpnII GATC 3 cut(s) 114, 1288, 1430
DriI GACNNNNNGTC 1 cut(s) 1271
Eam1105I GACNNNNNGTC 1 cut(s) 1271
Ecl136II GAGCTC 1 cut(s) 633
Eco24I GRGCYC 2 cut(s) 437, 635
Eco47I GGWCC 1 cut(s) 157
Eco47III AGCGCT 1 cut(s) 769
Eco53kI GAGCTC 1 cut(s) 633
Eco88I CYCGRG 1 cut(s) 782
EcoICRI GAGCTC 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 1062
EcoT38I GRGCYC 2 cut(s) 437, 635
FaeI CATG 5 cut(s) 361, 571, 802, 1318, 1546
FaqI GGGAC 3 cut(s) 858, 1173, 1214
FatI CATG 5 cut(s) 357, 567, 798, 1314, 1542
FauI CCCGC 1 cut(s) 835
Fnu4HI GCNGC 6 cut(s) 184, 318, 689, 738, 1008, 1191
FokI GGATG 1 cut(s) 1320
FriOI GRGCYC 2 cut(s) 437, 635
Fsp4HI GCNGC 6 cut(s) 184, 318, 689, 738, 1008, 1191
FspBI CTAG 5 cut(s) 641, 813, 1089, 1472, 1533
GlaI GCGC 2 cut(s) 537, 769
GluI GCNGC 6 cut(s) 184, 318, 689, 738, 1008, 1191
HaeII RGCGCY 1 cut(s) 771
HaeIII GGCC 1 cut(s) 1007
HapII CCGG 2 cut(s) 231, 385
HgaI GACGC 1 cut(s) 1366
HhaI GCGC 2 cut(s) 538, 770
Hin1II CATG 5 cut(s) 361, 571, 802, 1318, 1546
Hin6I GCGC 2 cut(s) 536, 768
HinP1I GCGC 2 cut(s) 536, 768
HindIII AAGCTT 2 cut(s) 212, 1369
HpaII CCGG 2 cut(s) 231, 385
HphI GGTGA 3 cut(s) 367, 478, 1472
Hpy188I TCNGA 7 cut(s) 108, 114, 192, 202, 276, 1277, 1462
Hpy188III TCNNGA 6 cut(s) 140, 526, 753, 1231, 1337, 1583
HpyAV CCTTC 4 cut(s) 271, 459, 577, 997
HpyCH4III ACNGT 6 cut(s) 607, 746, 851, 922, 1030, 1269
HpyCH4IV ACGT 1 cut(s) 660
HpyCH4V TGCA 9 cut(s) 335, 618, 649, 913, 957, 1148, 1167, 1193, 1217
HpyF10VI GCNNNNNNNGC 4 cut(s) 646, 662, 1016, 1292
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 1 cut(s) 660
Hsp92II CATG 5 cut(s) 361, 571, 802, 1318, 1546
HspAI GCGC 2 cut(s) 536, 768
KspI CCGCGG 1 cut(s) 402
Kzo9I GATC 3 cut(s) 114, 1288, 1430
LmnI GCTCC 2 cut(s) 1024, 1397
Lsp1109I GCAGC 4 cut(s) 195, 304, 724, 1177
LweI GCATC 1 cut(s) 1204
MaeI CTAG 5 cut(s) 641, 813, 1089, 1472, 1533
MaeII ACGT 1 cut(s) 660
MaeIII GTNAC 4 cut(s) 491, 925, 1030, 1379
MalI GATC 3 cut(s) 116, 1290, 1432
MbiI CCGCTC 1 cut(s) 656
MboI GATC 3 cut(s) 114, 1288, 1430
MboII GAAGA 7 cut(s) 427, 430, 556, 1366, 1427, 1508, 1579
MfeI CAATTG 2 cut(s) 307, 438
MflI RGATCY 1 cut(s) 1430
MhlI GDGCHC 4 cut(s) 437, 635, 783, 1088
MlyI GAGTC 4 cut(s) 488, 620, 1155, 1533
MmeI TCCRAC 1 cut(s) 1506
MseI TTAA 5 cut(s) 216, 252, 723, 933, 1203
MslI CAYNNNNRTG 3 cut(s) 566, 1338, 1485
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 2 cut(s) 231, 385
MspR9I CCNGG 1 cut(s) 1064
MunI CAATTG 2 cut(s) 307, 438
Mva1269I GAATGC 5 cut(s) 337, 562, 671, 1240, 1288
MvaI CCWGG 1 cut(s) 1064
MvnI CGCG 3 cut(s) 401, 536, 1010
MwoI GCNNNNNNNGC 4 cut(s) 646, 662, 1016, 1292
NdeII GATC 3 cut(s) 114, 1288, 1430
NlaIII CATG 5 cut(s) 361, 571, 802, 1318, 1546
NlaIV GGNNCC 1 cut(s) 1020
NmuCI GTSAC 1 cut(s) 491
NspI RCATGY 1 cut(s) 571
NspV TTCGAA 1 cut(s) 413
OliI CACNNNNGTG 1 cut(s) 1485
PaeR7I CTCGAG 1 cut(s) 782
PciI ACATGT 1 cut(s) 567
PctI GAATGC 5 cut(s) 337, 562, 671, 1240, 1288
PflMI CCANNNNNTGG 1 cut(s) 1411
PinAI ACCGGT 1 cut(s) 230
PkrI GCNGC 6 cut(s) 185, 319, 690, 739, 1009, 1192
PleI GAGTC 4 cut(s) 488, 619, 1155, 1533
PpsI GAGTC 4 cut(s) 488, 619, 1155, 1533
PpuMI RGGWCCY 1 cut(s) 157
PscI ACATGT 1 cut(s) 567
PsiI TTATAA 1 cut(s) 1143
Psp124BI GAGCTC 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 1062
PspGI CCWGG 1 cut(s) 1062
PspN4I GGNNCC 1 cut(s) 1020
PspPI GGNCC 1 cut(s) 157
PspPPI RGGWCCY 1 cut(s) 157
PspXI VCTCGAGB 1 cut(s) 782
PsrI GAACNNNNNNTAC 2 cut(s) 374, 406
PsuI RGATCY 1 cut(s) 1430
RsaI GTAC 4 cut(s) 5, 383, 407, 1058
RsaNI GTAC 4 cut(s) 4, 382, 406, 1057
RseI CAYNNNNRTG 3 cut(s) 566, 1338, 1485
SacI GAGCTC 1 cut(s) 635
SacII CCGCGG 1 cut(s) 402
SaqAI TTAA 5 cut(s) 216, 252, 723, 933, 1203
SatI GCNGC 6 cut(s) 184, 318, 689, 738, 1008, 1191
Sau3AI GATC 3 cut(s) 114, 1288, 1430
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 4 cut(s) 488, 620, 1155, 1533
ScrFI CCNGG 1 cut(s) 1064
SduI GDGCHC 4 cut(s) 437, 635, 783, 1088
SfaNI GCATC 1 cut(s) 1204
SfcI CTRYAG 1 cut(s) 676
Sfr274I CTCGAG 1 cut(s) 782
Sfr303I CCGCGG 1 cut(s) 402
SfuI TTCGAA 1 cut(s) 413
SgrBI CCGCGG 1 cut(s) 402
SinI GGWCC 1 cut(s) 157
SlaI CTCGAG 1 cut(s) 782
SmiMI CAYNNNNRTG 3 cut(s) 566, 1338, 1485
SmlI CTYRAG 3 cut(s) 296, 782, 1548
SmoI CTYRAG 3 cut(s) 296, 782, 1548
SsiI CCGC 9 cut(s) 240, 399, 401, 656, 688, 842, 1008, 1377, 1518
SspMI CTAG 5 cut(s) 641, 813, 1089, 1472, 1533
SstI GAGCTC 1 cut(s) 635
StyD4I CCNGG 1 cut(s) 1062
TaaI ACNGT 6 cut(s) 607, 746, 851, 922, 1030, 1269
TaiI ACGT 1 cut(s) 663
TaqI TCGA 8 cut(s) 44, 162, 227, 322, 345, 413, 783, 1098
TatI WGTACW 2 cut(s) 3, 405
TauI GCSGC 2 cut(s) 691, 1010
Tru1I TTAA 5 cut(s) 216, 252, 723, 933, 1203
Tru9I TTAA 5 cut(s) 216, 252, 723, 933, 1203
TscAI CASTG 5 cut(s) 642, 840, 854, 1155, 1274
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 4 cut(s) 183, 317, 737, 1190
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 3 cut(s) 596, 1016, 1356
TspRI CASTG 5 cut(s) 642, 840, 854, 1155, 1274
Van91I CCANNNNNTGG 1 cut(s) 1411
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 3 cut(s) 8, 15, 270
XceI RCATGY 1 cut(s) 571
XhoI CTCGAG 1 cut(s) 782
XspI CTAG 5 cut(s) 641, 813, 1089, 1472, 1533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.