pycom17g11970

UDP-arabinose 4-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0020218
Physical Location & Seq
Forward (+)
9770589 .. 9771564
976 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
ATGGATATTGCAGTCTCGAAACGGAGGAGGAACTTTTCCAGCAAATTTATCGCAGCTGCCGGCCTAATCACCCTCTGCATTGTCATATTCAAGCAGTCACATGATTCTAGCAGTCTCAACAAGTTTTCTTTTCATGAAGCAGGGATCAATCATGTCTTGGTCACAGGAGGAGCTGGCTACATAGGCTCTCATGCTGCACTTAGGCTCTTGAAGGACTCATGCCGTGTAACGATAGTGGACAATCTCTCTCGAGGAAACATCGGTGCTTTTAGGGTTCTTCAACAGTTGTTTCCAGAACCCGGGAGGCTTCAGTTCGTATACGCTGACCTCGGAGACGCAAAAGCTGTCAATAAAATCTTCTCAGAGAATGCATTTGATGCTGTCATGCATTTTGCAGCTGTTGCTTATGTTGGGGAAAGTACAGTCGAGCCTCTTCGATACTATCACAATATCACATCAAATACCCTGTTGGTTTTGGAGGCAATGGCAGCACACAATGTAAAAACATTGATCTATTCTAGTACTTGTGCAACTTATGGTGAACCTAAGAAGATGCCGATCACCGAAGAAACCGAGCAAGTGAGATTTCGTAACACAGTTTTAGTTGTCAGTTAA

Protein Analysis

205

Amino Acids

22.46

Weight (kDa)

8.99

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 51 - 191 9.1e-06 RmlD substrate binding domain
Epimerase PF01370 52 - 196 2e-30 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 52 - 175 3.2e-09 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 53 - 192 1.1e-29 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 53 - 196 6.9e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30620
pyrus_communis pycom17g11960 pycom17g11970
rosa_multiflora Rmu_sc0005702.1_g000001 Rmu_sc0022928.1_g000001
rosa_wichuraiana Rw2G041570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 318
AclWI GGATC 1 cut(s) 152
AcsI RAATTY 1 cut(s) 44
AcuI CTGAAG 1 cut(s) 293
AfaI GTAC 2 cut(s) 421, 523
AfiI CCNNNNNNNGG 1 cut(s) 299
AgsI TTSAA 3 cut(s) 91, 211, 281
AluBI AGCT 4 cut(s) 56, 173, 344, 398
AluI AGCT 4 cut(s) 56, 173, 344, 398
Alw26I GTCTC 3 cut(s) 19, 119, 327
AlwI GGATC 1 cut(s) 152
Ama87I CYCGRG 2 cut(s) 249, 299
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 5 cut(s) 53, 56, 194, 395, 488
ApoI RAATTY 1 cut(s) 44
AsuC2I CCSGG 2 cut(s) 300, 301
AsuHPI GGTGA 3 cut(s) 61, 551, 553
AvaI CYCGRG 2 cut(s) 249, 299
BbvI GCAGC 5 cut(s) 43, 65, 181, 407, 500
BceAI ACGGC 1 cut(s) 207
BcgI CGANNNNNNTGC 2 cut(s) 31, 65
BcnI CCSGG 2 cut(s) 300, 301
BcoDI GTCTC 3 cut(s) 19, 119, 327
BfaI CTAG 2 cut(s) 108, 519
BisI GCNGC 5 cut(s) 54, 57, 195, 396, 489
BlsI GCNGC 5 cut(s) 55, 58, 196, 397, 490
BmcAI AGTACT 1 cut(s) 523
Bme1390I CCNGG 2 cut(s) 300, 301
BmeT110I CYCGRG 2 cut(s) 249, 299
BmrFI CCNGG 2 cut(s) 300, 301
BmsI GCATC 2 cut(s) 367, 543
BpuMI CCSGG 2 cut(s) 300, 301
BsaBI GATNNNNATC 1 cut(s) 557
BsaJI CCNNGG 2 cut(s) 299, 328
Bsc4I CCNNNNNNNGG 1 cut(s) 299
Bse118I RCCGGY 1 cut(s) 59
Bse3DI GCAATG 1 cut(s) 489
Bse8I GATNNNNATC 1 cut(s) 557
BseDI CCNNGG 2 cut(s) 299, 328
BseJI GATNNNNATC 1 cut(s) 557
BseLI CCNNNNNNNGG 1 cut(s) 299
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 1 cut(s) 375
BseRI GAGGAG 2 cut(s) 40, 183
BseXI GCAGC 5 cut(s) 43, 65, 181, 407, 500
BsgI GTGCAG 1 cut(s) 180
BshFI GGCC 1 cut(s) 63
BsiHKCI CYCGRG 2 cut(s) 249, 299
BsiSI CCGG 2 cut(s) 60, 300
BslI CCNNNNNNNGG 1 cut(s) 299
BsmAI GTCTC 3 cut(s) 19, 119, 327
BsmBI CGTCTC 1 cut(s) 327
BsmI GAATGC 1 cut(s) 373
BsnI GGCC 1 cut(s) 63
BsoBI CYCGRG 2 cut(s) 249, 299
Bsp143I GATC 3 cut(s) 144, 510, 558
BspANI GGCC 1 cut(s) 63
BspCNI CTCAG 1 cut(s) 374
BspHI TCATGA 1 cut(s) 133
BspPI GGATC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 489
BsrFI RCCGGY 1 cut(s) 59
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 2 cut(s) 299, 328
BssMI GATC 3 cut(s) 144, 510, 558
BssNAI GTATAC 1 cut(s) 319
Bst1107I GTATAC 1 cut(s) 319
Bst4CI ACNGT 3 cut(s) 285, 424, 598
Bst6I CTCTTC 1 cut(s) 438
BstAPI GCANNNNNTGC 2 cut(s) 377, 401
BstC8I GCNNGC 2 cut(s) 61, 175
BstDEI CTNAG 3 cut(s) 200, 361, 546
BstKTI GATC 3 cut(s) 147, 513, 561
BstMAI GTCTC 3 cut(s) 19, 119, 327
BstMBI GATC 3 cut(s) 144, 510, 558
BstMWI GCNNNNNNNGC 4 cut(s) 183, 377, 401, 488
BstSCI CCNGG 2 cut(s) 298, 299
BstV1I GCAGC 5 cut(s) 43, 65, 181, 407, 500
BstZ17I GTATAC 1 cut(s) 319
BsuRI GGCC 1 cut(s) 63
Cac8I GCNNGC 2 cut(s) 61, 175
CciI TCATGA 1 cut(s) 133
Cfr10I RCCGGY 1 cut(s) 59
Cfr9I CCCGGG 1 cut(s) 299
CseI GACGC 1 cut(s) 344
Csp6I GTAC 2 cut(s) 420, 522
CviAII CATG 6 cut(s) 101, 134, 152, 191, 219, 385
CviQI GTAC 2 cut(s) 420, 522
DdeI CTNAG 3 cut(s) 200, 361, 546
DpnI GATC 3 cut(s) 146, 512, 560
DpnII GATC 3 cut(s) 144, 510, 558
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Eco57I CTGAAG 1 cut(s) 293
Eco88I CYCGRG 2 cut(s) 249, 299
EcoT22I ATGCAT 2 cut(s) 373, 390
Esp3I CGTCTC 1 cut(s) 327
FaeI CATG 6 cut(s) 104, 137, 155, 194, 222, 388
FatI CATG 6 cut(s) 100, 133, 151, 190, 218, 384
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 5 cut(s) 54, 57, 195, 396, 489
Fsp4HI GCNGC 5 cut(s) 54, 57, 195, 396, 489
FspBI CTAG 2 cut(s) 108, 519
GluI GCNGC 5 cut(s) 54, 57, 195, 396, 489
HaeIII GGCC 1 cut(s) 63
HapII CCGG 2 cut(s) 60, 300
HgaI GACGC 1 cut(s) 344
Hin1II CATG 6 cut(s) 104, 137, 155, 194, 222, 388
HinfI GANTC 2 cut(s) 104, 215
HpaII CCGG 2 cut(s) 60, 300
HphI GGTGA 3 cut(s) 61, 551, 553
Hpy166II GTNNAC 3 cut(s) 238, 319, 542
Hpy188I TCNGA 2 cut(s) 332, 364
Hpy188III TCNNGA 5 cut(s) 16, 134, 208, 249, 293
Hpy8I GTNNAC 3 cut(s) 238, 319, 542
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 3 cut(s) 285, 424, 598
HpyCH4V TGCA 7 cut(s) 11, 78, 197, 371, 388, 395, 530
HpyF10VI GCNNNNNNNGC 4 cut(s) 183, 377, 401, 488
HpyF3I CTNAG 3 cut(s) 200, 361, 546
Hsp92II CATG 6 cut(s) 104, 137, 155, 194, 222, 388
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
Kzo9I GATC 3 cut(s) 144, 510, 558
LmnI GCTCC 1 cut(s) 170
LpnPI CCDG 8 cut(s) 52, 73, 126, 150, 159, 306, 313, 479
Lsp1109I GCAGC 5 cut(s) 43, 65, 181, 407, 500
LweI GCATC 2 cut(s) 367, 543
MaeI CTAG 2 cut(s) 108, 519
MaeIII GTNAC 4 cut(s) 96, 160, 226, 590
MalI GATC 3 cut(s) 146, 512, 560
MboI GATC 3 cut(s) 144, 510, 558
MboII GAAGA 5 cut(s) 269, 349, 425, 562, 578
MluCI AATT 1 cut(s) 44
MlyI GAGTC 1 cut(s) 209
MnlI CCTC 9 cut(s) 18, 21, 83, 161, 245, 297, 338, 441, 472
Mph1103I ATGCAT 2 cut(s) 373, 390
MroNI GCCGGC 1 cut(s) 59
MseI TTAA 1 cut(s) 613
MspA1I CMGCKG 2 cut(s) 56, 398
MspI CCGG 2 cut(s) 60, 300
MspR9I CCNGG 2 cut(s) 300, 301
Mva1269I GAATGC 1 cut(s) 373
MwoI GCNNNNNNNGC 4 cut(s) 183, 377, 401, 488
NaeI GCCGGC 1 cut(s) 61
NciI CCSGG 2 cut(s) 300, 301
NdeII GATC 3 cut(s) 144, 510, 558
NgoMIV GCCGGC 1 cut(s) 59
NlaIII CATG 6 cut(s) 104, 137, 155, 194, 222, 388
NmuCI GTSAC 2 cut(s) 96, 160
NsiI ATGCAT 2 cut(s) 373, 390
PaeR7I CTCGAG 1 cut(s) 249
PagI TCATGA 1 cut(s) 133
PctI GAATGC 1 cut(s) 373
PdiI GCCGGC 1 cut(s) 61
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 5 cut(s) 55, 58, 196, 397, 490
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
PvuII CAGCTG 2 cut(s) 56, 398
RsaI GTAC 2 cut(s) 421, 523
RsaNI GTAC 2 cut(s) 420, 522
SaqAI TTAA 1 cut(s) 613
SatI GCNGC 5 cut(s) 54, 57, 195, 396, 489
Sau3AI GATC 3 cut(s) 144, 510, 558
ScaI AGTACT 1 cut(s) 523
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 2 cut(s) 300, 301
SetI ASST 6 cut(s) 58, 175, 330, 346, 400, 547
SfaNI GCATC 2 cut(s) 367, 543
Sfr274I CTCGAG 1 cut(s) 249
SlaI CTCGAG 1 cut(s) 249
SmaI CCCGGG 1 cut(s) 301
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
Sse9I AATT 1 cut(s) 44
SspMI CTAG 2 cut(s) 108, 519
StyD4I CCNGG 2 cut(s) 298, 299
TaaI ACNGT 3 cut(s) 285, 424, 598
TaqI TCGA 4 cut(s) 17, 250, 426, 436
TasI AATT 1 cut(s) 44
TatI WGTACW 2 cut(s) 419, 521
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 1 cut(s) 613
Tru9I TTAA 1 cut(s) 613
TseFI GTSAC 2 cut(s) 96, 160
TseI GCWGC 5 cut(s) 53, 56, 194, 395, 488
Tsp45I GTSAC 2 cut(s) 96, 160
TspDTI ATGAA 2 cut(s) 122, 150
TspGWI ACGGA 1 cut(s) 37
TspMI CCCGGG 1 cut(s) 299
XapI RAATTY 1 cut(s) 44
XhoI CTCGAG 1 cut(s) 249
XmaI CCCGGG 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 318
XspI CTAG 2 cut(s) 108, 519
ZrmI AGTACT 1 cut(s) 523
Zsp2I ATGCAT 2 cut(s) 373, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.