Rw2G041570

UDP-arabinose 4-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0020218
Physical Location & Seq
Reverse (-)
67601386 .. 67603580
2195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G041570.1

Sequence Viewer

Length: 873 bp
ATGGATTTTGCAGACTCGAAACGAAGGGGCAATGTTCCAGGCAGATTTTTGGCAGCTGCAGTCCTCATTACTGTTTGCATTTTCGTCTACAAGCAGTCACTTAACTCCAGCAATGAACGCAACCTGTTCTCTTATCACGAAGCAGGAGTAACACATGTATTGGTTACTGGAGGTGCTGGCTACATAGGCTCTCATGCTGCACTTAGGCTGCTGAAGGATTCATATCGTGTAACCATAGTGGATAATCTTTCTCGAGGAAATATTGGTGCTGTTAGGATTCTTCAGCAGTTATTTCCAGAACCTGGGAGGCTTCAGTTTGTTAAAGCTGACCTGGGAGATGCAAAAGCTGTCAATAAAATCTTCTCAGAAAATGCATTTGATGCTGTTATGCATTTTGCAGCTGTTGCTTATGTTGGTGAAAGTACACTTGAACCTCTTAGGTATTATCATAATATCACATCAAATACTCTATTGGTTTTGGAGGCAATGGCAGCACACAATGTAAAGACATTGATCTATTCTAGTACTTGTGCCACATACGGCGAACCCAAGACAATGCCCATTACGGAAGAAACTAAGCAAGTTCCAATCAATCCATACGGAAAAGCCAAGAAGATGGCAGAAGATATCATACTGGATTTCTCCAAAAACTCAAACATGGCAGTCATGATCTTACGATATTTTAATGTTATAGGATCAGATCCTGAGGGAAGATTAGGAGAGGCTCCTCGTCCTGAGCTACGTGAGCAGGGCCGAATATCTGGAGCTTGTTTTGATGCAGCTCGAGGAATTATTTCTGGACTGAAGGTACTTCATCTCCTTCCACTTCCACCCTTCTCTCCTGGAGTTGTGAAGGGAAAAATCCTCTACTAG

Protein Analysis

290

Amino Acids

31.75

Weight (kDa)

9.12

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 52 - 233 2.4e-13 RmlD substrate binding domain
Epimerase PF01370 53 - 234 4.9e-42 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 53 - 233 2.9e-13 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 54 - 232 7.1e-42 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 54 - 215 1.1e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 55 - 237 7.2e-07 Male sterility protein
NAD_binding_10 PF13460 57 - 230 9e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30620
pyrus_communis pycom17g11960 pycom17g11970
rosa_multiflora Rmu_sc0005702.1_g000001 Rmu_sc0022928.1_g000001
rosa_wichuraiana Rw2G041570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 302
AccI GTMKAC 1 cut(s) 87
AclWI GGATC 2 cut(s) 695, 703
AcuI CTGAAG 4 cut(s) 233, 266, 296, 824
AfaI GTAC 3 cut(s) 424, 526, 810
AfiI CCNNNNNNNGG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 1 cut(s) 431
AjnI CCWGG 4 cut(s) 37, 301, 330, 841
AluBI AGCT 7 cut(s) 56, 326, 347, 401, 739, 767, 782
AluI AGCT 7 cut(s) 56, 326, 347, 401, 739, 767, 782
AlwI GGATC 2 cut(s) 695, 703
AlwNI CAGNNNCTG 2 cut(s) 302, 704
Ama87I CYCGRG 2 cut(s) 252, 783
AoxI GGCC 1 cut(s) 751
ApeKI GCWGC 7 cut(s) 53, 56, 197, 208, 398, 491, 779
Asp700I GAANNNNTTC 1 cut(s) 793
AspS9I GGNCC 1 cut(s) 751
AsuHPI GGTGA 1 cut(s) 428
AvaI CYCGRG 2 cut(s) 252, 783
AxyI CCTNAGG 1 cut(s) 705
BarI GAAGNNNNNNTAC 2 cut(s) 615, 647
BbvI GCAGC 7 cut(s) 43, 65, 184, 195, 410, 503, 791
BccI CCATC 1 cut(s) 610
BceAI ACGGC 1 cut(s) 556
BciT130I CCWGG 4 cut(s) 39, 303, 332, 843
BfaI CTAG 2 cut(s) 522, 871
BfmI CTRYAG 1 cut(s) 57
BisI GCNGC 7 cut(s) 54, 57, 198, 209, 399, 492, 780
BlsI GCNGC 7 cut(s) 55, 58, 199, 210, 400, 493, 781
BmcAI AGTACT 1 cut(s) 526
Bme1390I CCNGG 4 cut(s) 39, 303, 332, 843
BmeT110I CYCGRG 2 cut(s) 252, 783
BmgT120I GGNCC 1 cut(s) 751
BmiI GGNNCC 1 cut(s) 726
BmrFI CCNGG 4 cut(s) 39, 303, 332, 843
BmsI GCATC 3 cut(s) 328, 370, 766
BpmI CTGGAG 4 cut(s) 91, 189, 783, 864
Bpu10I CCTNAGC 1 cut(s) 735
BsaAI YACGTR 1 cut(s) 743
BsaBI GATNNNNATC 1 cut(s) 222
BsaJI CCNNGG 2 cut(s) 302, 331
BsaXI ACNNNNNCTCC 2 cut(s) 801, 831
Bsc4I CCNNNNNNNGG 1 cut(s) 302
Bse1I ACTGG 2 cut(s) 172, 639
Bse21I CCTNAGG 1 cut(s) 705
Bse3DI GCAATG 3 cut(s) 37, 118, 492
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 4 cut(s) 39, 303, 332, 843
BseDI CCNNGG 2 cut(s) 302, 331
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 1 cut(s) 302
BseMI GCAATG 3 cut(s) 37, 118, 492
BseMII CTCAG 3 cut(s) 378, 696, 726
BseNI ACTGG 2 cut(s) 172, 639
BseRI GAGGAG 1 cut(s) 717
BseXI GCAGC 7 cut(s) 43, 65, 184, 195, 410, 503, 791
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 1 cut(s) 753
BsiHKCI CYCGRG 2 cut(s) 252, 783
BslI CCNNNNNNNGG 1 cut(s) 302
BsnI GGCC 1 cut(s) 753
BsoBI CYCGRG 2 cut(s) 252, 783
Bsp143I GATC 4 cut(s) 513, 669, 695, 700
BspANI GGCC 1 cut(s) 753
BspCNI CTCAG 3 cut(s) 377, 697, 727
BspHI TCATGA 1 cut(s) 666
BspLI GGNNCC 1 cut(s) 726
BspMAI CTGCAG 1 cut(s) 61
BspPI GGATC 2 cut(s) 695, 703
BsrDI GCAATG 3 cut(s) 37, 118, 492
BsrI ACTGG 2 cut(s) 172, 639
BssECI CCNNGG 2 cut(s) 302, 331
BssMI GATC 4 cut(s) 513, 669, 695, 700
Bst2UI CCWGG 4 cut(s) 39, 303, 332, 843
Bst4CI ACNGT 1 cut(s) 73
BstAPI GCANNNNNTGC 2 cut(s) 380, 404
BstBAI YACGTR 1 cut(s) 743
BstC8I GCNNGC 1 cut(s) 178
BstDEI CTNAG 6 cut(s) 203, 364, 437, 576, 705, 735
BstKTI GATC 4 cut(s) 516, 672, 698, 703
BstMBI GATC 4 cut(s) 513, 669, 695, 700
BstMWI GCNNNNNNNGC 6 cut(s) 117, 186, 380, 404, 491, 745
BstNI CCWGG 4 cut(s) 39, 303, 332, 843
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 4 cut(s) 37, 301, 330, 841
BstSFI CTRYAG 1 cut(s) 57
BstV1I GCAGC 7 cut(s) 43, 65, 184, 195, 410, 503, 791
BstX2I RGATCY 1 cut(s) 700
BstXI CCANNNNNNTGG 1 cut(s) 616
BstYI RGATCY 1 cut(s) 700
Bsu36I CCTNAGG 1 cut(s) 705
BsuRI GGCC 1 cut(s) 753
Cac8I GCNNGC 1 cut(s) 178
CaiI CAGNNNCTG 2 cut(s) 302, 704
CciI TCATGA 1 cut(s) 666
Cfr13I GGNCC 1 cut(s) 751
Csp6I GTAC 3 cut(s) 423, 525, 809
CviAII CATG 4 cut(s) 155, 194, 658, 667
CviQI GTAC 3 cut(s) 423, 525, 809
DdeI CTNAG 6 cut(s) 203, 364, 437, 576, 705, 735
DpnI GATC 4 cut(s) 515, 671, 697, 702
DpnII GATC 4 cut(s) 513, 669, 695, 700
Eco32I GATATC 1 cut(s) 628
Eco57I CTGAAG 4 cut(s) 233, 266, 296, 824
Eco81I CCTNAGG 1 cut(s) 705
Eco88I CYCGRG 2 cut(s) 252, 783
EcoRII CCWGG 4 cut(s) 37, 301, 330, 841
EcoRV GATATC 1 cut(s) 628
EcoT22I ATGCAT 2 cut(s) 376, 393
FaeI CATG 4 cut(s) 158, 197, 661, 670
FatI CATG 4 cut(s) 154, 193, 657, 666
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 7 cut(s) 54, 57, 198, 209, 399, 492, 780
Fsp4HI GCNGC 7 cut(s) 54, 57, 198, 209, 399, 492, 780
FspBI CTAG 2 cut(s) 522, 871
GluI GCNGC 7 cut(s) 54, 57, 198, 209, 399, 492, 780
GsuI CTGGAG 4 cut(s) 91, 189, 783, 864
HaeIII GGCC 1 cut(s) 753
Hin1II CATG 4 cut(s) 158, 197, 661, 670
HinfI GANTC 3 cut(s) 14, 218, 277
HphI GGTGA 1 cut(s) 428
Hpy166II GTNNAC 2 cut(s) 88, 425
Hpy188I TCNGA 2 cut(s) 367, 700
Hpy188III TCNNGA 8 cut(s) 137, 252, 296, 667, 704, 734, 762, 798
Hpy8I GTNNAC 2 cut(s) 88, 425
HpyAV CCTTC 6 cut(s) 18, 208, 799, 830, 844, 847
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 1 cut(s) 742
HpyCH4V TGCA 9 cut(s) 11, 59, 78, 200, 341, 374, 391, 398, 779
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 186, 380, 404, 491, 745
HpyF3I CTNAG 6 cut(s) 203, 364, 437, 576, 705, 735
HpySE526I ACGT 1 cut(s) 742
Hsp92II CATG 4 cut(s) 158, 197, 661, 670
Kzo9I GATC 4 cut(s) 513, 669, 695, 700
LmnI GCTCC 2 cut(s) 730, 764
Lsp1109I GCAGC 7 cut(s) 43, 65, 184, 195, 410, 503, 791
LweI GCATC 3 cut(s) 328, 370, 766
MaeI CTAG 2 cut(s) 522, 871
MaeII ACGT 1 cut(s) 742
MaeIII GTNAC 4 cut(s) 96, 148, 163, 229
MalI GATC 4 cut(s) 515, 671, 697, 702
MboI GATC 4 cut(s) 513, 669, 695, 700
MboII GAAGA 6 cut(s) 272, 352, 581, 625, 635, 723
MflI RGATCY 1 cut(s) 700
MluCI AATT 1 cut(s) 789
MlyI GAGTC 1 cut(s) 8
Mph1103I ATGCAT 2 cut(s) 376, 393
MroXI GAANNNNTTC 1 cut(s) 793
MseI TTAA 3 cut(s) 102, 321, 684
MspA1I CMGCKG 2 cut(s) 56, 401
MspR9I CCNGG 4 cut(s) 39, 303, 332, 843
MvaI CCWGG 4 cut(s) 39, 303, 332, 843
MwoI GCNNNNNNNGC 6 cut(s) 117, 186, 380, 404, 491, 745
NdeII GATC 4 cut(s) 513, 669, 695, 700
NlaIII CATG 4 cut(s) 158, 197, 661, 670
NlaIV GGNNCC 1 cut(s) 726
NmuCI GTSAC 1 cut(s) 96
NsiI ATGCAT 2 cut(s) 376, 393
NspI RCATGY 1 cut(s) 158
PaeR7I CTCGAG 2 cut(s) 252, 783
PagI TCATGA 1 cut(s) 666
PciI ACATGT 1 cut(s) 154
PdmI GAANNNNTTC 1 cut(s) 793
PfeI GAWTC 2 cut(s) 218, 277
PflMI CCANNNNNTGG 1 cut(s) 302
PfoI TCCNGGA 1 cut(s) 841
PkrI GCNGC 7 cut(s) 55, 58, 199, 210, 400, 493, 781
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
Ppu21I YACGTR 1 cut(s) 743
PscI ACATGT 1 cut(s) 154
Psp6I CCWGG 4 cut(s) 37, 301, 330, 841
PspGI CCWGG 4 cut(s) 37, 301, 330, 841
PspN4I GGNNCC 1 cut(s) 726
PspPI GGNCC 1 cut(s) 751
PspXI VCTCGAGB 1 cut(s) 783
PstI CTGCAG 1 cut(s) 61
PstNI CAGNNNCTG 2 cut(s) 302, 704
PsuI RGATCY 1 cut(s) 700
PvuII CAGCTG 2 cut(s) 56, 401
RsaI GTAC 3 cut(s) 424, 526, 810
RsaNI GTAC 3 cut(s) 423, 525, 809
SaqAI TTAA 3 cut(s) 102, 321, 684
SatI GCNGC 7 cut(s) 54, 57, 198, 209, 399, 492, 780
Sau3AI GATC 4 cut(s) 513, 669, 695, 700
Sau96I GGNCC 1 cut(s) 751
ScaI AGTACT 1 cut(s) 526
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 4 cut(s) 39, 303, 332, 843
SfaNI GCATC 3 cut(s) 328, 370, 766
SfcI CTRYAG 1 cut(s) 57
Sfr274I CTCGAG 2 cut(s) 252, 783
SlaI CTCGAG 2 cut(s) 252, 783
SmlI CTYRAG 2 cut(s) 252, 783
SmoI CTYRAG 2 cut(s) 252, 783
Sse9I AATT 1 cut(s) 789
SspI AATATT 1 cut(s) 262
SspMI CTAG 2 cut(s) 522, 871
StyD4I CCNGG 4 cut(s) 37, 301, 330, 841
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 1 cut(s) 745
TaqI TCGA 3 cut(s) 17, 253, 784
TasI AATT 1 cut(s) 789
TatI WGTACW 2 cut(s) 422, 524
TfiI GAWTC 2 cut(s) 218, 277
Tru1I TTAA 3 cut(s) 102, 321, 684
Tru9I TTAA 3 cut(s) 102, 321, 684
TseFI GTSAC 1 cut(s) 96
TseI GCWGC 7 cut(s) 53, 56, 197, 208, 398, 491, 779
Tsp45I GTSAC 1 cut(s) 96
TspDTI ATGAA 3 cut(s) 129, 210, 803
TspGWI ACGGA 2 cut(s) 581, 615
Van91I CCANNNNNTGG 1 cut(s) 302
XceI RCATGY 1 cut(s) 158
XhoI CTCGAG 2 cut(s) 252, 783
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 1 cut(s) 793
XspI CTAG 2 cut(s) 522, 871
ZrmI AGTACT 1 cut(s) 526
Zsp2I ATGCAT 2 cut(s) 376, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.