pycom17g13510

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0017343
Physical Location & Seq
Forward (+)
11126640 .. 11128301
1662 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1587 bp
ATGCTGAGTGTGAAGTTTTTTGCGTATGCATTTTCTGCGCAGAGAAATTTTAGCACCAAAAGTAGTGGAAAAAGCATAAATTGTGTTCAAGATAAATACAAATTCAGACCCAAACCCAAAAACCCTAGAGTTAAGTGGACTGAACAGCAGAAACAAATCATATCTGCCATTTCTGAGGGGAAATCTGTGTTCATTACCGGTTCCGCGGGGACCGGGAAAACAATGTTGGTTAAGCACATTATAAAACTGTTGAAGAGATGTCACACACCTTCAAAGGTTTTTTTCACGGCACCTACTGGTGTTGCGGCTTGTGCAATCAGTGGGCAGACCCTTCACTCTTTCGCCGGTATTGGTTGTGGCAATGATGATAGGGATACTTTGCTGAGTAGGGTTCTCTCGAATAAGGAGGCTTGTAGGAAGTGGTCGAAAGCCGAGGCGTTGGTCATAGATGAAATTAGCATGGTTGACGGGGAGTTGCTTGATAGTCTTGAATACATTGCAAAATACATACGACAAATTGATGAAATTTGGGGTGGAATGCAGCTTGTTGTGGGTGGGGATTTCTTTCAGTTACCACCCGTAAAAACTGAGTGGAATTCAGAGAGTAAGGAATTTGCATTTGAAGCAGACTGTTGGGAATCTAGCTTTGATTTACAAGTTAATCTTACAAAGGTTTTTCGGCAGTCAGACCCTCAACTGATCAGGATACTTCAGGGTATTAGAACAGGGGAGATTGATCCTGAAGACATGAAGTTTTTAGAAAATTCTTGCGCAGATTCTGAGCCAGATCCGTCTGCAGTACAATTCTATCCAAGGATTCGAGACGTAAATAGAGTGAATGCAAGTAGATTGGAGAGCTTGGGTAATGAACTTGTTGTTTATACTTCAGTTGATAGTGGTCACGATCCTTGGAAGAGACAGCTTGAACAGGTGATTGCGCCTAAAGAAATTGGCTTATGTAAAGATGCAAGGGTGATGCTGCTAAAAAATTTGAACACTCAGCGTGGCCTGGTAAATGGTGCTACTGGTACTGTTATGGCATTTCGTAAGACCAAAGATGTGGGATTGACAAGCATATGTGATGGTGGGCTGCTACCAATAGTTAAATTTGATTCGGGGATAATAAAGGTGATAAAACCAAAAACATGGGATGTGACAGAGGGAGATTCAGTAGTTGCTCAAAGAGAGCAGCTACCTCTCACACTGGCTTGGGCTTCAAGCATTCACAAGTGCCAGGGCATGACTCTGGACCGTCTTTACACCGATCAAAAGAGCACCCAGGGAATGGTTTATGTAACTCTTTCCCGTGTGAAAAGCTTGGAAGGCCTGTATATATCCGGTTTCCATCCCAGCAAGATTAAGGTGCACCTTAAGGTTAAGCAGTTTTATAAAAGGTTTTCTGGCGAACACGAAAAGGAGGTTGAGGATAATGTCACCCAGAAAAAGTATCGTAGCAATCATAATGGTGGATGGGGAAAAAAAGATGAGGAGGTTGAGAATGATAATGCCATCCAGAAAAAATACCGTAGCAATGATATCGGCAGAAAAGGAAACGATGGCAGCGGATACACATTGGAACAACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PIF1

Protein Analysis

529

Amino Acids

59.23

Weight (kDa)

8.96

Isoelectric Point (pI)

29.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIF1 PF05970 47 - 231 1.4e-40 PIF1-like helicase
AAA_30 PF13604 47 - 254 4.1e-14 AAA domain
AAA_19 PF13245 50 - 194 5.2e-16 AAA domain
AAA_22 PF13401 60 - 167 1e-07 AAA domain
Pif1_2B_dom PF21530 312 - 348 2.4e-07 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017343)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g37811
prunus_persica Prupe.3G170200_v2.0.a1
pyrus_communis pycom09g07450 pycom17g13510
rosa_laevigata RLG00000020517
rosa_multiflora Rmu_sc0000185.1_g000025
rosa_roxburghii Rroxscaffold_2G00096840
rosa_rugosa Rorug02G0423300
rosa_samantha Rh2AG483700 Rh2BG496000 Rh2CG469900 Rh2DG507000
rosa_wichuraiana Rw2G039640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 242, 1389
Acc16I TGCGCA 2 cut(s) 39, 772
AccB1I GGYRCC 1 cut(s) 289
AccB7I CCANNNNNTGG 1 cut(s) 1285
AccII CGCG 1 cut(s) 206
AciI CCGC 4 cut(s) 204, 206, 305, 1563
AclWI GGATC 3 cut(s) 731, 782, 899
AcsI RAATTY 8 cut(s) 46, 101, 525, 595, 611, 763, 988, 1106
AcuI CTGAAG 3 cut(s) 695, 762, 870
AfaI GTAC 2 cut(s) 801, 1030
AfiI CCNNNNNNNGG 1 cut(s) 1285
AflII CTTAAG 1 cut(s) 1370
AgeI ACCGGT 1 cut(s) 197
AgsI TTSAA 8 cut(s) 89, 253, 273, 491, 623, 926, 994, 1218
AjnI CCWGG 3 cut(s) 1008, 1233, 1278
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AluBI AGCT 6 cut(s) 544, 645, 858, 922, 1192, 1317
AluI AGCT 6 cut(s) 544, 645, 858, 922, 1192, 1317
Alw21I GWGCWC 2 cut(s) 1277, 1368
Alw26I GTCTC 2 cut(s) 816, 910
Alw44I GTGCAC 1 cut(s) 1364
AlwI GGATC 3 cut(s) 731, 782, 899
AlwNI CAGNNNCTG 1 cut(s) 779
AoxI GGCC 2 cut(s) 1006, 1324
ApaLI GTGCAC 1 cut(s) 1364
ApeKI GCWGC 5 cut(s) 541, 979, 1090, 1189, 1560
ApoI RAATTY 8 cut(s) 46, 101, 525, 595, 611, 763, 988, 1106
AsiGI ACCGGT 1 cut(s) 197
AspLEI GCGC 3 cut(s) 40, 773, 940
AspS9I GGNCC 2 cut(s) 210, 1249
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 4 cut(s) 943, 985, 1141, 1426
AvaII GGWCC 2 cut(s) 210, 1249
BaeGI GKGCMC 1 cut(s) 1368
BanI GGYRCC 1 cut(s) 289
BbsI GAAGAC 1 cut(s) 750
Bbv12I GWGCWC 2 cut(s) 1277, 1368
BbvI GCAGC 5 cut(s) 553, 966, 1077, 1201, 1572
BccI CCATC 5 cut(s) 1076, 1353, 1464, 1517, 1550
BceAI ACGGC 1 cut(s) 303
BcgI CGANNNNNNTGC 2 cut(s) 1519, 1553
BciT130I CCWGG 3 cut(s) 1010, 1235, 1280
BciVI GTATCC 3 cut(s) 367, 699, 1559
BclI TGATCA 1 cut(s) 699
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 2 cut(s) 816, 910
BfaI CTAG 2 cut(s) 126, 642
BfmI CTRYAG 2 cut(s) 795, 1583
BfrI CTTAAG 1 cut(s) 1370
BfuI GTATCC 3 cut(s) 367, 699, 1559
BisI GCNGC 6 cut(s) 306, 542, 980, 1091, 1190, 1561
BlsI GCNGC 6 cut(s) 307, 543, 981, 1092, 1191, 1562
Bme1390I CCNGG 4 cut(s) 214, 1010, 1235, 1280
Bme18I GGWCC 2 cut(s) 210, 1249
BmgT120I GGNCC 2 cut(s) 210, 1249
BmiI GGNNCC 3 cut(s) 202, 211, 291
BmrFI CCNGG 4 cut(s) 214, 1010, 1235, 1280
BmsI GCATC 2 cut(s) 955, 966
BpiI GAAGAC 1 cut(s) 750
BpuMI CCSGG 1 cut(s) 214
BsaJI CCNNGG 7 cut(s) 204, 432, 812, 908, 1234, 1278, 1279
BsaWI WCCGGW 2 cut(s) 197, 1337
BsaXI ACNNNNNCTCC 2 cut(s) 1155, 1185
Bsc4I CCNNNNNNNGG 1 cut(s) 1285
Bse118I RCCGGY 2 cut(s) 197, 344
Bse1I ACTGG 3 cut(s) 301, 1030, 1209
Bse3DI GCAATG 3 cut(s) 367, 495, 1537
BseBI CCWGG 3 cut(s) 1010, 1235, 1280
BseDI CCNNGG 7 cut(s) 204, 432, 812, 908, 1234, 1278, 1279
BseGI GGATG 4 cut(s) 1156, 1345, 1475, 1509
BseLI CCNNNNNNNGG 1 cut(s) 1285
BseMI GCAATG 3 cut(s) 367, 495, 1537
BseMII CTCAG 5 cut(s) 165, 374, 579, 771, 1013
BseNI ACTGG 3 cut(s) 301, 1030, 1209
BseRI GAGGAG 1 cut(s) 1502
BseSI GKGCMC 1 cut(s) 1368
BseXI GCAGC 5 cut(s) 553, 966, 1077, 1201, 1572
BseYI CCCAGC 1 cut(s) 1349
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 2 cut(s) 1008, 1326
BshNI GGYRCC 1 cut(s) 289
BshTI ACCGGT 1 cut(s) 197
BsiHKAI GWGCWC 2 cut(s) 1277, 1368
BsiSI CCGG 4 cut(s) 198, 213, 345, 1338
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 1 cut(s) 1285
BsmAI GTCTC 2 cut(s) 816, 910
BsmBI CGTCTC 1 cut(s) 816
BsmFI GGGAC 1 cut(s) 223
BsmI GAATGC 3 cut(s) 543, 844, 1221
BsnI GGCC 2 cut(s) 1008, 1326
Bsp1286I GDGCHC 2 cut(s) 1277, 1368
Bsp143I GATC 5 cut(s) 699, 736, 787, 904, 1264
BspACI CCGC 4 cut(s) 204, 206, 305, 1563
BspANI GGCC 2 cut(s) 1008, 1326
BspCNI CTCAG 5 cut(s) 166, 375, 580, 772, 1012
BspFNI CGCG 1 cut(s) 206
BspLI GGNNCC 3 cut(s) 202, 211, 291
BspMAI CTGCAG 1 cut(s) 799
BspPI GGATC 3 cut(s) 731, 782, 899
BspT107I GGYRCC 1 cut(s) 289
BspTI CTTAAG 1 cut(s) 1370
BsrDI GCAATG 3 cut(s) 367, 495, 1537
BsrFI RCCGGY 2 cut(s) 197, 344
BsrI ACTGG 3 cut(s) 301, 1030, 1209
BssAI RCCGGY 2 cut(s) 197, 344
BssECI CCNNGG 7 cut(s) 204, 432, 812, 908, 1234, 1278, 1279
BssMI GATC 5 cut(s) 699, 736, 787, 904, 1264
BssT1I CCWWGG 2 cut(s) 812, 908
Bst2UI CCWGG 3 cut(s) 1010, 1235, 1280
Bst4CI ACNGT 6 cut(s) 249, 632, 1033, 1253, 1526, 1584
Bst6I CTCTTC 2 cut(s) 248, 908
BstAFI CTTAAG 1 cut(s) 1370
BstAPI GCANNNNNTGC 1 cut(s) 35
BstDEI CTNAG 6 cut(s) 5, 174, 383, 588, 780, 999
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 4 cut(s) 1156, 1345, 1475, 1509
BstFNI CGCG 1 cut(s) 206
BstHHI GCGC 3 cut(s) 40, 773, 940
BstKTI GATC 5 cut(s) 702, 739, 790, 907, 1267
BstMAI GTCTC 2 cut(s) 816, 910
BstMBI GATC 5 cut(s) 699, 736, 787, 904, 1264
BstMWI GCNNNNNNNGC 4 cut(s) 35, 311, 623, 1323
BstNI CCWGG 3 cut(s) 1010, 1235, 1280
BstSCI CCNGG 4 cut(s) 212, 1008, 1233, 1278
BstSFI CTRYAG 2 cut(s) 795, 1583
BstSLI GKGCMC 1 cut(s) 1368
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 5 cut(s) 553, 966, 1077, 1201, 1572
BstV2I GAAGAC 1 cut(s) 750
BstX2I RGATCY 1 cut(s) 787
BstXI CCANNNNNNTGG 2 cut(s) 1060, 1146
BstYI RGATCY 1 cut(s) 787
BsuI GTATCC 3 cut(s) 367, 699, 1559
BsuRI GGCC 2 cut(s) 1008, 1326
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 4 cut(s) 1156, 1345, 1475, 1509
BtsIMutI CAGTG 2 cut(s) 325, 1202
CaiI CAGNNNCTG 1 cut(s) 779
CfoI GCGC 3 cut(s) 40, 773, 940
Cfr10I RCCGGY 2 cut(s) 197, 344
Cfr13I GGNCC 2 cut(s) 210, 1249
Cfr42I CCGCGG 1 cut(s) 207
Csp6I GTAC 2 cut(s) 800, 1029
CspAI ACCGGT 1 cut(s) 197
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviAII CATG 4 cut(s) 460, 748, 1146, 1240
CviQI GTAC 2 cut(s) 800, 1029
DdeI CTNAG 6 cut(s) 5, 174, 383, 588, 780, 999
DpnI GATC 5 cut(s) 701, 738, 789, 906, 1266
DpnII GATC 5 cut(s) 699, 736, 787, 904, 1264
Eam1104I CTCTTC 2 cut(s) 248, 908
EarI CTCTTC 2 cut(s) 248, 908
Eco130I CCWWGG 2 cut(s) 812, 908
Eco147I AGGCCT 1 cut(s) 1326
Eco32I GATATC 1 cut(s) 1537
Eco47I GGWCC 2 cut(s) 210, 1249
Eco57I CTGAAG 3 cut(s) 695, 762, 870
EcoRI GAATTC 1 cut(s) 595
EcoRII CCWGG 3 cut(s) 1008, 1233, 1278
EcoRV GATATC 1 cut(s) 1537
EcoT14I CCWWGG 2 cut(s) 812, 908
EcoT22I ATGCAT 1 cut(s) 31
ErhI CCWWGG 2 cut(s) 812, 908
Esp3I CGTCTC 1 cut(s) 816
FaeI CATG 4 cut(s) 463, 751, 1149, 1243
FalI AAGNNNNNCTT 2 cut(s) 648, 680
FaqI GGGAC 1 cut(s) 223
FatI CATG 4 cut(s) 459, 747, 1145, 1239
FauI CCCGC 1 cut(s) 199
FauNDI CATATG 1 cut(s) 1076
FbaI TGATCA 1 cut(s) 699
Fnu4HI GCNGC 6 cut(s) 306, 542, 980, 1091, 1190, 1561
FokI GGATG 4 cut(s) 1163, 1332, 1482, 1496
Fsp4HI GCNGC 6 cut(s) 306, 542, 980, 1091, 1190, 1561
FspBI CTAG 2 cut(s) 126, 642
FspI TGCGCA 2 cut(s) 39, 772
GlaI GCGC 3 cut(s) 39, 772, 939
GluI GCNGC 6 cut(s) 306, 542, 980, 1091, 1190, 1561
GsaI CCCAGC 1 cut(s) 1353
HaeIII GGCC 2 cut(s) 1008, 1326
HapII CCGG 4 cut(s) 198, 213, 345, 1338
HhaI GCGC 3 cut(s) 40, 773, 940
Hin1II CATG 4 cut(s) 463, 751, 1149, 1243
Hin6I GCGC 3 cut(s) 38, 771, 938
HinP1I GCGC 3 cut(s) 38, 771, 938
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HindIII AAGCTT 1 cut(s) 1315
HinfI GANTC 6 cut(s) 638, 776, 817, 1112, 1166, 1243
HpaII CCGG 4 cut(s) 198, 213, 345, 1338
HphI GGTGA 4 cut(s) 943, 985, 1141, 1426
Hpy166II GTNNAC 3 cut(s) 138, 466, 1366
Hpy188I TCNGA 5 cut(s) 107, 175, 601, 688, 781
Hpy188III TCNNGA 9 cut(s) 89, 397, 488, 703, 740, 821, 902, 1247, 1513
Hpy8I GTNNAC 3 cut(s) 138, 466, 1366
HpyAV CCTTC 3 cut(s) 279, 341, 1316
HpyCH4III ACNGT 6 cut(s) 249, 632, 1033, 1253, 1526, 1584
HpyCH4IV ACGT 1 cut(s) 825
HpyCH4V TGCA 9 cut(s) 29, 314, 500, 541, 617, 797, 842, 968, 1366
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 311, 623, 1323
HpyF3I CTNAG 6 cut(s) 5, 174, 383, 588, 780, 999
HpySE526I ACGT 1 cut(s) 825
Hsp92II CATG 4 cut(s) 463, 751, 1149, 1243
HspAI GCGC 3 cut(s) 38, 771, 938
Ksp22I TGATCA 1 cut(s) 699
KspI CCGCGG 1 cut(s) 207
Kzo9I GATC 5 cut(s) 699, 736, 787, 904, 1264
Lsp1109I GCAGC 5 cut(s) 553, 966, 1077, 1201, 1572
LweI GCATC 2 cut(s) 955, 966
MaeI CTAG 2 cut(s) 126, 642
MaeII ACGT 1 cut(s) 825
MaeIII GTNAC 6 cut(s) 260, 570, 899, 1153, 1294, 1432
MalI GATC 5 cut(s) 701, 738, 789, 906, 1266
MboI GATC 5 cut(s) 699, 736, 787, 904, 1264
MboII GAAGA 3 cut(s) 265, 755, 925
MflI RGATCY 1 cut(s) 787
MhlI GDGCHC 2 cut(s) 1277, 1368
MlyI GAGTC 1 cut(s) 1237
Mph1103I ATGCAT 1 cut(s) 31
MseI TTAA 7 cut(s) 132, 231, 660, 1104, 1359, 1371, 1377
MslI CAYNNNNRTG 1 cut(s) 1464
MspA1I CMGCKG 2 cut(s) 206, 1563
MspCI CTTAAG 1 cut(s) 1370
MspI CCGG 4 cut(s) 198, 213, 345, 1338
MspR9I CCNGG 4 cut(s) 214, 1010, 1235, 1280
Mva1269I GAATGC 3 cut(s) 543, 844, 1221
MvaI CCWGG 3 cut(s) 1010, 1235, 1280
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 4 cut(s) 35, 311, 623, 1323
NciI CCSGG 1 cut(s) 214
NdeI CATATG 1 cut(s) 1076
NdeII GATC 5 cut(s) 699, 736, 787, 904, 1264
NlaIII CATG 4 cut(s) 463, 751, 1149, 1243
NlaIV GGNNCC 3 cut(s) 202, 211, 291
NmeAIII GCCGAG 1 cut(s) 457
NmuCI GTSAC 4 cut(s) 260, 899, 1153, 1432
NsbI TGCGCA 2 cut(s) 39, 772
NsiI ATGCAT 1 cut(s) 31
PasI CCCWGGG 1 cut(s) 1279
PceI AGGCCT 1 cut(s) 1326
PctI GAATGC 3 cut(s) 543, 844, 1221
PfeI GAWTC 5 cut(s) 638, 776, 817, 1112, 1166
PflMI CCANNNNNTGG 1 cut(s) 1285
PinAI ACCGGT 1 cut(s) 197
PkrI GCNGC 6 cut(s) 307, 543, 981, 1092, 1191, 1562
PleI GAGTC 1 cut(s) 1237
PpsI GAGTC 1 cut(s) 1237
PsiI TTATAA 2 cut(s) 242, 1389
Psp6I CCWGG 3 cut(s) 1008, 1233, 1278
PspFI CCCAGC 1 cut(s) 1349
PspGI CCWGG 3 cut(s) 1008, 1233, 1278
PspN4I GGNNCC 3 cut(s) 202, 211, 291
PspPI GGNCC 2 cut(s) 210, 1249
PstI CTGCAG 1 cut(s) 799
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 1 cut(s) 787
RsaI GTAC 2 cut(s) 801, 1030
RsaNI GTAC 2 cut(s) 800, 1029
RseI CAYNNNNRTG 1 cut(s) 1464
SacII CCGCGG 1 cut(s) 207
SaqAI TTAA 7 cut(s) 132, 231, 660, 1104, 1359, 1371, 1377
SatI GCNGC 6 cut(s) 306, 542, 980, 1091, 1190, 1561
Sau3AI GATC 5 cut(s) 699, 736, 787, 904, 1264
Sau96I GGNCC 2 cut(s) 210, 1249
SchI GAGTC 1 cut(s) 1237
ScrFI CCNGG 4 cut(s) 214, 1010, 1235, 1280
SduI GDGCHC 2 cut(s) 1277, 1368
SfaNI GCATC 2 cut(s) 955, 966
SfcI CTRYAG 2 cut(s) 795, 1583
Sfr303I CCGCGG 1 cut(s) 207
SgrBI CCGCGG 1 cut(s) 207
SinI GGWCC 2 cut(s) 210, 1249
SmiMI CAYNNNNRTG 1 cut(s) 1464
SmlI CTYRAG 1 cut(s) 1370
SmoI CTYRAG 1 cut(s) 1370
SseBI AGGCCT 1 cut(s) 1326
SsiI CCGC 4 cut(s) 204, 206, 305, 1563
SspMI CTAG 2 cut(s) 126, 642
StuI AGGCCT 1 cut(s) 1326
StyD4I CCNGG 4 cut(s) 212, 1008, 1233, 1278
StyI CCWWGG 2 cut(s) 812, 908
TaaI ACNGT 6 cut(s) 249, 632, 1033, 1253, 1526, 1584
TaiI ACGT 1 cut(s) 828
TaqI TCGA 3 cut(s) 398, 425, 820
TatI WGTACW 1 cut(s) 799
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 5 cut(s) 638, 776, 817, 1112, 1166
Tru1I TTAA 7 cut(s) 132, 231, 660, 1104, 1359, 1371, 1377
Tru9I TTAA 7 cut(s) 132, 231, 660, 1104, 1359, 1371, 1377
TscAI CASTG 2 cut(s) 325, 1209
TseFI GTSAC 4 cut(s) 260, 899, 1153, 1432
TseI GCWGC 5 cut(s) 541, 979, 1090, 1189, 1560
Tsp45I GTSAC 4 cut(s) 260, 899, 1153, 1432
TspDTI ATGAA 5 cut(s) 181, 465, 537, 764, 882
TspGWI ACGGA 1 cut(s) 780
TspRI CASTG 2 cut(s) 325, 1209
Van91I CCANNNNNTGG 1 cut(s) 1285
Vha464I CTTAAG 1 cut(s) 1370
VneI GTGCAC 1 cut(s) 1364
VpaK11BI GGWCC 2 cut(s) 210, 1249
XapI RAATTY 8 cut(s) 46, 101, 525, 595, 611, 763, 988, 1106
XspI CTAG 2 cut(s) 126, 642
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.