pycom17g22490

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0016890
Physical Location & Seq
Forward (+)
20855296 .. 20856003
708 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 708 bp
ATGTCGATGTCGACATGGCTCTGCACTAAAAGCACCAAGAACATGCTTGTCAAAATTGTTCACCCCGGAGGGCATGTGGAGCTGCACGATAGTCCTCTTCTCGCAGTGGAGATCATGCTAAGGAATCCGCAATGTTGCGTTGCGCACCCTCACATTTTCCAGCAGCCATGGGCGATTGTGGAGCCGGATACCATGCTAAGTCTAGGGCAAAAGTACTACGTCGTGCCCGTAAGCACGATTCGGAAGCTTCAAAGGCTCTCCATAAAGTATTCACCCACTTCAATTCATGACACTCAAAGAAATGTGACGCCCCCACCAAGCAAAGAAGATGAGAAACGTGATGACATGCTTTCTAATTATTGTAATTGGTTTTACGCAAGGAATAAAAAATCTTCCAAGTTTTCGTATGCTTGTGTTTGTAACCATGAGGAAAACAATGTGAAGGAGGGAGGATGTTTTTCTGAAGATAATTGCTTTGCTTGCTTTTTTAAGGGGGCAAAGATTAAAGAAAATGGCGGCGATCACGATTCGAAAGAGGAAACAGAATCATTAAGCAGCAGCATTTCATCTTCTGAGATTGAGACAAAGACGCTTACAAGGAAGAGGAACAGGAATTCTACAAAGCACTGGGGCAAAGATTCAACACCTAAAAGATTGTCATCCCTAGATCATTGGCAGCCAAGTCTAGAGAGCATCCATGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

236

Amino Acids

26.87

Weight (kDa)

8.04

Isoelectric Point (pI)

59.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 7 - 234 3.5e-22 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016890)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28900
malus_domestica MD17G1220000.v1.1
prunus_persica Prupe.3G095700_v2.0.a1
pyrus_communis pycom17g22490
rosa_laevigata RLG00000019340
rosa_multiflora Rmu_co8401069.1_g000001 Rmu_sc0016125.1_g000001 Rmu_sc0017323.1_g000012
rosa_rugosa Rorug02G0312000
rosa_samantha Rh2AG364800 Rh2BG370500 Rh2CG348000 Rh2DG387400
rosa_wichuraiana Rw2G029730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 144
AccI GTMKAC 1 cut(s) 11
AciI CCGC 2 cut(s) 128, 516
AcsI RAATTY 1 cut(s) 613
AcuI CTGAAG 1 cut(s) 483
AcyI GRCGYC 1 cut(s) 308
AfaI GTAC 1 cut(s) 215
AgsI TTSAA 3 cut(s) 251, 282, 642
AluBI AGCT 2 cut(s) 82, 247
AluI AGCT 2 cut(s) 82, 247
Alw26I GTCTC 1 cut(s) 575
ApeKI GCWGC 5 cut(s) 82, 163, 555, 558, 676
ApoI RAATTY 1 cut(s) 613
AspLEI GCGC 1 cut(s) 145
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 2 cut(s) 53, 264
AsuII TTCGAA 1 cut(s) 530
BaeGI GKGCMC 1 cut(s) 228
BbvI GCAGC 5 cut(s) 69, 175, 567, 570, 688
BciVI GTATCC 1 cut(s) 181
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 575
BfaI CTAG 3 cut(s) 203, 665, 686
BfuI GTATCC 1 cut(s) 181
BisI GCNGC 6 cut(s) 83, 164, 517, 556, 559, 677
BlsI GCNGC 6 cut(s) 84, 165, 518, 557, 560, 678
BmcAI AGTACT 1 cut(s) 215
Bme1390I CCNGG 1 cut(s) 66
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 1 cut(s) 66
BmrI ACTGGG 1 cut(s) 637
BmsI GCATC 1 cut(s) 702
BmuI ACTGGG 1 cut(s) 637
Bpu10I CCTNAGC 1 cut(s) 119
Bpu14I TTCGAA 1 cut(s) 530
BpuMI CCSGG 1 cut(s) 66
BsaBI GATNNNNATC 1 cut(s) 658
BsaHI GRCGYC 1 cut(s) 308
BsaJI CCNNGG 2 cut(s) 64, 167
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bse1I ACTGG 1 cut(s) 632
Bse3DI GCAATG 1 cut(s) 137
Bse8I GATNNNNATC 1 cut(s) 658
BseDI CCNNGG 2 cut(s) 64, 167
BseGI GGATG 3 cut(s) 458, 659, 693
BseJI GATNNNNATC 1 cut(s) 658
BseMI GCAATG 1 cut(s) 137
BseMII CTCAG 1 cut(s) 564
BseNI ACTGG 1 cut(s) 632
BseSI GKGCMC 1 cut(s) 228
BseXI GCAGC 5 cut(s) 69, 175, 567, 570, 688
BsgI GTGCAG 2 cut(s) 7, 68
BsiSI CCGG 2 cut(s) 66, 185
BsmAI GTCTC 1 cut(s) 575
Bsp119I TTCGAA 1 cut(s) 530
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 3 cut(s) 111, 520, 667
Bsp19I CCATGG 1 cut(s) 167
BspACI CCGC 2 cut(s) 128, 516
BspCNI CTCAG 1 cut(s) 565
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 183
BspT104I TTCGAA 1 cut(s) 530
BsrDI GCAATG 1 cut(s) 137
BsrI ACTGG 1 cut(s) 632
BssECI CCNNGG 2 cut(s) 64, 167
BssMI GATC 3 cut(s) 111, 520, 667
BssNI GRCGYC 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 167
Bst6I CTCTTC 2 cut(s) 102, 596
BstACI GRCGYC 1 cut(s) 308
BstBI TTCGAA 1 cut(s) 530
BstC8I GCNNGC 1 cut(s) 481
BstDEI CTNAG 3 cut(s) 119, 197, 573
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 3 cut(s) 458, 659, 693
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 3 cut(s) 114, 523, 670
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 3 cut(s) 111, 520, 667
BstMWI GCNNNNNNNGC 4 cut(s) 30, 79, 253, 480
BstNSI RCATGY 3 cut(s) 46, 77, 349
BstSCI CCNGG 1 cut(s) 64
BstSLI GKGCMC 1 cut(s) 228
BstV1I GCAGC 5 cut(s) 69, 175, 567, 570, 688
BsuI GTATCC 1 cut(s) 181
BtgI CCRYGG 1 cut(s) 167
BtsCI GGATG 3 cut(s) 458, 659, 693
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 111, 625
Cac8I GCNNGC 1 cut(s) 481
CciI TCATGA 1 cut(s) 286
CfoI GCGC 1 cut(s) 145
CseI GACGC 2 cut(s) 316, 598
Csp6I GTAC 1 cut(s) 214
CviJI RGCY 7 cut(s) 19, 82, 166, 184, 247, 256, 679
CviKI_1 RGCY 7 cut(s) 19, 82, 166, 184, 247, 256, 679
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 3 cut(s) 119, 197, 573
DpnI GATC 3 cut(s) 113, 522, 669
DpnII GATC 3 cut(s) 111, 520, 667
Eam1104I CTCTTC 2 cut(s) 102, 596
EarI CTCTTC 2 cut(s) 102, 596
Eco130I CCWWGG 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 483
EcoRI GAATTC 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 167
ErhI CCWWGG 1 cut(s) 167
FblI GTMKAC 1 cut(s) 11
Fnu4HI GCNGC 6 cut(s) 83, 164, 517, 556, 559, 677
FokI GGATG 3 cut(s) 465, 646, 680
Fsp4HI GCNGC 6 cut(s) 83, 164, 517, 556, 559, 677
FspBI CTAG 3 cut(s) 203, 665, 686
FspI TGCGCA 1 cut(s) 144
GlaI GCGC 1 cut(s) 144
GluI GCNGC 6 cut(s) 83, 164, 517, 556, 559, 677
HapII CCGG 2 cut(s) 66, 185
HgaI GACGC 2 cut(s) 316, 598
HhaI GCGC 1 cut(s) 145
Hin1I GRCGYC 1 cut(s) 308
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 5 cut(s) 124, 238, 527, 545, 638
HpaII CCGG 2 cut(s) 66, 185
HphI GGTGA 2 cut(s) 53, 264
Hpy166II GTNNAC 2 cut(s) 12, 61
Hpy188I TCNGA 3 cut(s) 243, 463, 574
Hpy188III TCNNGA 3 cut(s) 287, 524, 686
Hpy8I GTNNAC 2 cut(s) 12, 61
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 1 cut(s) 436
HpyCH4IV ACGT 2 cut(s) 219, 337
HpyCH4V TGCA 2 cut(s) 24, 85
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 79, 253, 480
HpyF3I CTNAG 3 cut(s) 119, 197, 573
HpySE526I ACGT 2 cut(s) 219, 337
Hsp92I GRCGYC 1 cut(s) 308
HspAI GCGC 1 cut(s) 143
Kzo9I GATC 3 cut(s) 111, 520, 667
LmnI GCTCC 2 cut(s) 79, 181
LpnPI CCDG 5 cut(s) 79, 173, 198, 595, 613
Lsp1109I GCAGC 5 cut(s) 69, 175, 567, 570, 688
LweI GCATC 1 cut(s) 702
MaeI CTAG 3 cut(s) 203, 665, 686
MaeII ACGT 2 cut(s) 219, 337
MaeIII GTNAC 2 cut(s) 304, 419
MalI GATC 3 cut(s) 113, 522, 669
MboI GATC 3 cut(s) 111, 520, 667
MboII GAAGA 6 cut(s) 89, 338, 384, 476, 561, 613
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 6 cut(s) 54, 282, 355, 364, 469, 613
MnlI CCTC 8 cut(s) 62, 105, 159, 421, 439, 443, 529, 597
MseI TTAA 3 cut(s) 489, 504, 551
MspI CCGG 2 cut(s) 66, 185
MspR9I CCNGG 1 cut(s) 66
MwoI GCNNNNNNNGC 4 cut(s) 30, 79, 253, 480
NciI CCSGG 1 cut(s) 66
NcoI CCATGG 1 cut(s) 167
NdeII GATC 3 cut(s) 111, 520, 667
NlaIV GGNNCC 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 304
NsbI TGCGCA 1 cut(s) 144
NspI RCATGY 3 cut(s) 46, 77, 349
NspV TTCGAA 1 cut(s) 530
PagI TCATGA 1 cut(s) 286
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PfeI GAWTC 5 cut(s) 124, 238, 527, 545, 638
PkrI GCNGC 6 cut(s) 84, 165, 518, 557, 560, 678
PspN4I GGNNCC 1 cut(s) 183
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SalI GTCGAC 1 cut(s) 10
SaqAI TTAA 3 cut(s) 489, 504, 551
SatI GCNGC 6 cut(s) 83, 164, 517, 556, 559, 677
Sau3AI GATC 3 cut(s) 111, 520, 667
ScaI AGTACT 1 cut(s) 215
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 1 cut(s) 228
SetI ASST 5 cut(s) 84, 222, 249, 340, 649
SfaNI GCATC 1 cut(s) 702
SfuI TTCGAA 1 cut(s) 530
Sse9I AATT 6 cut(s) 54, 282, 355, 364, 469, 613
SsiI CCGC 2 cut(s) 128, 516
SspMI CTAG 3 cut(s) 203, 665, 686
StyD4I CCNGG 1 cut(s) 64
StyI CCWWGG 1 cut(s) 167
TaiI ACGT 2 cut(s) 222, 340
TaqI TCGA 3 cut(s) 5, 11, 530
TasI AATT 6 cut(s) 54, 282, 355, 364, 469, 613
TatI WGTACW 1 cut(s) 213
TauI GCSGC 1 cut(s) 519
TfiI GAWTC 5 cut(s) 124, 238, 527, 545, 638
Tru1I TTAA 3 cut(s) 489, 504, 551
Tru9I TTAA 3 cut(s) 489, 504, 551
TscAI CASTG 2 cut(s) 111, 632
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 5 cut(s) 82, 163, 555, 558, 676
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 2 cut(s) 275, 555
TspRI CASTG 2 cut(s) 111, 632
XapI RAATTY 1 cut(s) 613
XbaI TCTAGA 1 cut(s) 685
XceI RCATGY 3 cut(s) 46, 77, 349
XmiI GTMKAC 1 cut(s) 11
XspI CTAG 3 cut(s) 203, 665, 686
ZrmI AGTACT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.