pycom17g25510

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0014551
Physical Location & Seq
Reverse (-)
23628378 .. 23631813
3436 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1716 bp
ATGGGTGAATCAACCACAACCGCACTCGATGGCAATCAGATCGATCGCCAAGGCCCTTGCCCGGTTCGGCGTCGACCGCATGTTCGGAGTCGTGGGAATTCCCGTCACCTCGCTCGCGAACCGCGCGGTTTCGCTCGGAGTTCGGTTTATCGCCTTCCACAACGAGCAGTCGGCGGGTTACGCCGCCTCAGCCTACGGATATCTAACGGGTCGACCCGGAGTCCTCCTCACGGTCTCGGGTCCCGGATGCGTCCACGGGCTCGCGGGTCTTTCCAACGCCATGGCCAATGCTTGGCCCATGGTGATGATCGATCGAGTTCGTGCGACCAGAAAGATTTCGGCCGCGGAGATTTCCAGGAGCTCGATCAGATCGCCGCCGTCGAACCCTTTTCCAAATTCTCCGCCAAAGCTAAATCAATCAAAGAAATACCGGATTGCGTGTTTCAAGCTCTTGCAAAAGCCGGTTCGGGTCGACCCGGAGGGTGCTATTTGGATTTTCCTTCGGATGTACTGCACCAGACAATTTCTGAGGCGGAGGCTGAGAGCTTATTGGCCACCGCCGCCGAGAAATTCGGAGAGTCGGAGAAGGTTCCGTGTGTGCAGAATTCGCAGATTGAGGAGGCCGTTTCGCTGCTCCGGCACGCGGAGAGGCCGTTGATTGTTTTTGGTAAAGGAGCTGCATTTGCTCGAGCGGAGAACGAGTTGGGTAAGCTGGTGGAGAGGACGGGAATCCCATTTTTGCCCACTCCAATGGGGAAGGGGCTGTTGCCGGACACTCATGAGCTGGCGGCGACCGCGGCAAGGTCTCTGGCGATCGGGAAGTGTGACGTGGTGCTTGTCGTTGGCGCAAGGCTTAACTGGCTTTTGCACTTTGGTGAGCCGCCCAAGTGGTCCAAGGATGTGAAGTTCATTTTGGTTGATGTTAGTAAGGAAGAGATTGAGCTGCGAAAACCACATTTGGGTTTGGTTGGAGATGCAAAGTTGGTGTTGGAGAAGATCAATTTGGGGATCAAGGATGACCCTTTTTGTTTGGGGAAGTCTCATCCGTGGGTTGCAGCGATTTTGAGTAAGGTGAAGGACAATGTTTCGAAAATGGAGGCTCAGTTGGCTAAGGAAGTTGTGCCATTTAATTTCTTGACGCCGATGAAGATTATTAGAGATGCAATTGCAGGGTTGGGGAGCCCTGCTCCTATATTGGTTTCCGAGGGGGCTAACACTATGGATGTGGGTCGGTCTGTGTTGGTTCAGACCGAACCGAGGACCAGGTTGGATGCAGGGACTTGGGGAACAATGGGGGTTGGATTGGGTTACTGCATTGCGGGTGCGGTAGCTTCTCCTGACCGGCTTGTTGTTGCAGTGGAAGGGGATTCAGGATTTGGGTTCAGTGCAATGGAAGTTGAGACATTGGTTCGATACCAGTTACCTGTTGTTGTGATAGTCTTTAACAATGGTGGTGTATATGGTGGTGATCGAAGAAGCCCAGATGAAATTAGTGGGCCTTACAAGGATGACCCTGCTCCCACTTCCTTCGTCCCAAGTGCGGGTTATCACACTCTGATTGAAGCCTTTGGAGGCAAAGGCTATCTTGTTGGGACACCTGAAGAACTCAAGTCCGCCCTTTCAGAATCCTTCTCGGCACGAAAACCAGCTGTTATAAATGTAATTATCGATCCTTATGCTGGTGCAGAAAGTGGGAGGCTTCAACACAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

572

Amino Acids

61.48

Weight (kDa)

8.82

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_M PF00205 205 - 333 7.4e-35 Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C PF02775 406 - 554 2.4e-25 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17380
fragaria_vesca FvH4_1g28990
malus_domestica MD00G1054500.v1.1 MD09G1258100.v1.1
prunus_persica Prupe.3G150000_v2.0.a1
pyrus_communis pycom09g17290 pycom17g25510
rosa_chinensis RchiOBHm_Chr3g0494041
rosa_laevigata RLG00000022686
rosa_multiflora Rmu_sc0003412.1_g000008
rosa_roxburghii Rroxscaffold_6G00391310
rosa_rugosa Rorug03G0263700
rosa_samantha Rh3AG313400 Rh3BG349500 Rh3CG346300 Rh3DG348800
rosa_wichuraiana Rw3G027430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1655
AccB7I CCANNNNNTGG 1 cut(s) 292
AccBSI CCGCTC 1 cut(s) 692
AccI GTMKAC 3 cut(s) 73, 212, 472
AccII CGCG 7 cut(s) 117, 124, 126, 264, 345, 644, 797
AclWI GGATC 2 cut(s) 1016, 1664
AcoI YGGCCR 3 cut(s) 283, 340, 552
AcsI RAATTY 4 cut(s) 97, 395, 569, 604
AcuI CTGAAG 1 cut(s) 1620
AcyI GRCGYC 2 cut(s) 70, 1139
AfaI GTAC 1 cut(s) 510
AgsI TTSAA 3 cut(s) 446, 1562, 1703
AhdI GACNNNNNGTC 1 cut(s) 219
AjiI CACGTC 1 cut(s) 829
AjnI CCWGG 2 cut(s) 354, 1262
AjuI GAANNNNNNNTTGG 4 cut(s) 896, 928, 986, 1018
AleI CACNNNNGTG 1 cut(s) 873
AloI GAACNNNNNNTCC 2 cut(s) 890, 922
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 4 cut(s) 239, 810, 1044, 1394
AlwI GGATC 2 cut(s) 1016, 1664
Ama87I CYCGRG 2 cut(s) 236, 687
AoxI GGCC 8 cut(s) 52, 283, 294, 340, 552, 621, 650, 1496
ApeKI GCWGC 4 cut(s) 631, 677, 943, 1055
ApoI RAATTY 4 cut(s) 97, 395, 569, 604
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
Asp700I GAANNNNTTC 1 cut(s) 335
AspLEI GCGC 2 cut(s) 126, 848
AspS9I GGNCC 6 cut(s) 53, 240, 295, 891, 1260, 1496
AsuC2I CCSGG 4 cut(s) 62, 217, 244, 477
AsuHPI GGTGA 6 cut(s) 17, 98, 314, 887, 1084, 1478
AsuII TTCGAA 1 cut(s) 1088
AvaI CYCGRG 2 cut(s) 236, 687
AvaII GGWCC 3 cut(s) 240, 891, 1260
BalI TGGCCA 2 cut(s) 285, 554
BanII GRGCYC 3 cut(s) 262, 363, 1184
Bbv12I GWGCWC 1 cut(s) 363
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 4 cut(s) 618, 664, 930, 1067
BccI CCATC 1 cut(s) 23
BceAI ACGGC 3 cut(s) 362, 608, 637
BcgI CGANNNNNNTGC 4 cut(s) 22, 56, 1658, 1692
BciT130I CCWGG 2 cut(s) 356, 1264
BcnI CCSGG 4 cut(s) 62, 217, 244, 477
BcoDI GTCTC 4 cut(s) 239, 810, 1044, 1394
Bme1390I CCNGG 6 cut(s) 62, 217, 244, 356, 477, 1264
Bme18I GGWCC 3 cut(s) 240, 891, 1260
BmeRI GACNNNNNGTC 1 cut(s) 219
BmeT110I CYCGRG 2 cut(s) 236, 687
BmgBI CACGTC 1 cut(s) 829
BmgT120I GGNCC 6 cut(s) 53, 240, 295, 891, 1260, 1496
BmiI GGNNCC 4 cut(s) 241, 242, 591, 1181
BmrFI CCNGG 6 cut(s) 62, 217, 244, 356, 477, 1264
BmsI GCATC 4 cut(s) 237, 964, 1150, 1261
BplI GAGNNNNNCTC 4 cut(s) 211, 243, 1171, 1203
Bpu10I CCTNAGC 2 cut(s) 188, 1110
Bpu14I TTCGAA 1 cut(s) 1088
BpuEI CTTGAG 1 cut(s) 1592
BpuMI CCSGG 4 cut(s) 62, 217, 244, 477
Bsa29I ATCGAT 3 cut(s) 42, 310, 1668
BsaBI GATNNNNATC 1 cut(s) 33
BsaHI GRCGYC 2 cut(s) 70, 1139
BsaI GGTCTC 2 cut(s) 239, 810
BsaWI WCCGGW 1 cut(s) 430
BsaXI ACNNNNNCTCC 6 cut(s) 638, 668, 686, 716, 1088, 1118
Bse118I RCCGGY 2 cut(s) 461, 1341
Bse1I ACTGG 2 cut(s) 863, 1417
Bse3DI GCAATG 2 cut(s) 1314, 1395
Bse8I GATNNNNATC 1 cut(s) 33
BseBI CCWGG 2 cut(s) 356, 1264
BseCI ATCGAT 3 cut(s) 42, 310, 1668
BseGI GGATG 8 cut(s) 252, 511, 904, 1021, 1042, 1228, 1276, 1513
BseJI GATNNNNATC 1 cut(s) 33
BseMI GCAATG 2 cut(s) 1314, 1395
BseMII CTCAG 4 cut(s) 202, 519, 531, 1115
BseNI ACTGG 2 cut(s) 863, 1417
BseRI GAGGAG 2 cut(s) 216, 632
BseX3I CGGCCG 1 cut(s) 340
BseXI GCAGC 4 cut(s) 618, 664, 930, 1067
BsgI GTGCAG 3 cut(s) 497, 620, 1704
Bsh1236I CGCG 7 cut(s) 117, 124, 126, 264, 345, 644, 797
Bsh1285I CGRYCG 6 cut(s) 46, 77, 314, 343, 795, 816
BshFI GGCC 8 cut(s) 54, 285, 296, 342, 554, 623, 652, 1498
BshVI ATCGAT 3 cut(s) 42, 310, 1668
BsiEI CGRYCG 6 cut(s) 46, 77, 314, 343, 795, 816
BsiHKAI GWGCWC 1 cut(s) 363
BsiHKCI CYCGRG 2 cut(s) 236, 687
BsiSI CCGG 9 cut(s) 62, 217, 244, 431, 462, 477, 637, 770, 1342
BslFI GGGAC 4 cut(s) 226, 1291, 1517, 1606
BsmAI GTCTC 4 cut(s) 239, 810, 1044, 1394
BsmFI GGGAC 4 cut(s) 226, 1291, 1517, 1606
BsnI GGCC 8 cut(s) 54, 285, 296, 342, 554, 623, 652, 1498
Bso31I GGTCTC 2 cut(s) 239, 810
BsoBI CYCGRG 2 cut(s) 236, 687
Bsp119I TTCGAA 1 cut(s) 1088
Bsp1286I GDGCHC 3 cut(s) 262, 363, 1184
Bsp19I CCATGG 2 cut(s) 280, 298
Bsp68I TCGCGA 1 cut(s) 117
BspANI GGCC 8 cut(s) 54, 285, 296, 342, 554, 623, 652, 1498
BspCNI CTCAG 4 cut(s) 201, 520, 532, 1114
BspDI ATCGAT 3 cut(s) 42, 310, 1668
BspFNI CGCG 7 cut(s) 117, 124, 126, 264, 345, 644, 797
BspHI TCATGA 1 cut(s) 778
BspLI GGNNCC 4 cut(s) 241, 242, 591, 1181
BspPI GGATC 2 cut(s) 1016, 1664
BspT104I TTCGAA 1 cut(s) 1088
BspTNI GGTCTC 2 cut(s) 239, 810
BsrBI CCGCTC 1 cut(s) 692
BsrDI GCAATG 2 cut(s) 1314, 1395
BsrFI RCCGGY 2 cut(s) 461, 1341
BsrI ACTGG 2 cut(s) 863, 1417
BssAI RCCGGY 2 cut(s) 461, 1341
BssNI GRCGYC 2 cut(s) 70, 1139
BssT1I CCWWGG 4 cut(s) 49, 280, 298, 894
Bst2UI CCWGG 2 cut(s) 356, 1264
Bst4CI ACNGT 1 cut(s) 233
Bst6I CTCTTC 1 cut(s) 927
BstACI GRCGYC 2 cut(s) 70, 1139
BstBI TTCGAA 1 cut(s) 1088
BstC8I GCNNGC 4 cut(s) 115, 262, 642, 786
BstDEI CTNAG 5 cut(s) 188, 528, 540, 1101, 1110
BstDSI CCRYGG 6 cut(s) 254, 280, 298, 343, 795, 1046
BstF5I GGATG 8 cut(s) 252, 511, 904, 1021, 1042, 1228, 1276, 1513
BstFNI CGCG 7 cut(s) 117, 124, 126, 264, 345, 644, 797
BstHHI GCGC 2 cut(s) 126, 848
BstMAI GTCTC 4 cut(s) 239, 810, 1044, 1394
BstMCI CGRYCG 6 cut(s) 46, 77, 314, 343, 795, 816
BstNI CCWGG 2 cut(s) 356, 1264
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 6 cut(s) 60, 215, 242, 354, 475, 1262
BstUI CGCG 7 cut(s) 117, 124, 126, 264, 345, 644, 797
BstV1I GCAGC 4 cut(s) 618, 664, 930, 1067
BstXI CCANNNNNNTGG 2 cut(s) 281, 751
BstZI CGGCCG 1 cut(s) 340
Bsu15I ATCGAT 3 cut(s) 42, 310, 1668
BsuRI GGCC 8 cut(s) 54, 285, 296, 342, 554, 623, 652, 1498
BsuTUI ATCGAT 3 cut(s) 42, 310, 1668
BtgI CCRYGG 6 cut(s) 254, 280, 298, 343, 795, 1046
BtrI CACGTC 1 cut(s) 829
BtsCI GGATG 8 cut(s) 252, 511, 904, 1021, 1042, 1228, 1276, 1513
BtsI GCAGTG 1 cut(s) 1362
BtsIMutI CAGTG 2 cut(s) 1362, 1390
BtuMI TCGCGA 1 cut(s) 117
Cac8I GCNNGC 4 cut(s) 115, 262, 642, 786
CciI TCATGA 1 cut(s) 778
CfoI GCGC 2 cut(s) 126, 848
Cfr10I RCCGGY 2 cut(s) 461, 1341
Cfr13I GGNCC 6 cut(s) 53, 240, 295, 891, 1260, 1496
Cfr42I CCGCGG 2 cut(s) 346, 798
ClaI ATCGAT 3 cut(s) 42, 310, 1668
CseI GACGC 3 cut(s) 59, 239, 1147
CsiI ACCWGGT 1 cut(s) 1262
Csp6I GTAC 1 cut(s) 509
CviAII CATG 4 cut(s) 80, 281, 299, 779
CviQI GTAC 1 cut(s) 509
DdeI CTNAG 5 cut(s) 188, 528, 540, 1101, 1110
DriI GACNNNNNGTC 1 cut(s) 219
EaeI YGGCCR 3 cut(s) 283, 340, 552
EagI CGGCCG 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 927
Eam1105I GACNNNNNGTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 927
EciI GGCGGA 3 cut(s) 391, 548, 1603
Ecl136II GAGCTC 1 cut(s) 361
EclXI CGGCCG 1 cut(s) 340
Eco130I CCWWGG 4 cut(s) 49, 280, 298, 894
Eco24I GRGCYC 3 cut(s) 262, 363, 1184
Eco31I GGTCTC 2 cut(s) 239, 810
Eco32I GATATC 1 cut(s) 201
Eco47I GGWCC 3 cut(s) 240, 891, 1260
Eco52I CGGCCG 1 cut(s) 340
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 1620
Eco88I CYCGRG 2 cut(s) 236, 687
EcoICRI GAGCTC 1 cut(s) 361
EcoO109I RGGNCCY 2 cut(s) 53, 240
EcoRI GAATTC 2 cut(s) 97, 604
EcoRII CCWGG 2 cut(s) 354, 1262
EcoRV GATATC 1 cut(s) 201
EcoT14I CCWWGG 4 cut(s) 49, 280, 298, 894
EcoT38I GRGCYC 3 cut(s) 262, 363, 1184
ErhI CCWWGG 4 cut(s) 49, 280, 298, 894
FaeI CATG 4 cut(s) 83, 284, 302, 782
FalI AAGNNNNNCTT 2 cut(s) 1569, 1601
FaqI GGGAC 4 cut(s) 226, 1291, 1517, 1606
FatI CATG 4 cut(s) 79, 280, 298, 778
FauI CCCGC 4 cut(s) 167, 257, 1312, 1534
FblI GTMKAC 3 cut(s) 73, 212, 472
FokI GGATG 8 cut(s) 259, 518, 911, 1028, 1029, 1235, 1283, 1520
FriOI GRGCYC 3 cut(s) 262, 363, 1184
GlaI GCGC 2 cut(s) 125, 847
HaeIII GGCC 8 cut(s) 54, 285, 296, 342, 554, 623, 652, 1498
HapII CCGG 9 cut(s) 62, 217, 244, 431, 462, 477, 637, 770, 1342
HgaI GACGC 3 cut(s) 59, 239, 1147
HhaI GCGC 2 cut(s) 126, 848
Hin1I GRCGYC 2 cut(s) 70, 1139
Hin1II CATG 4 cut(s) 83, 284, 302, 782
Hin6I GCGC 2 cut(s) 124, 846
HinP1I GCGC 2 cut(s) 124, 846
HincII GTYRAC 3 cut(s) 74, 213, 473
HindII GTYRAC 3 cut(s) 74, 213, 473
HinfI GANTC 7 cut(s) 8, 88, 220, 578, 729, 1367, 1625
HpaII CCGG 9 cut(s) 62, 217, 244, 431, 462, 477, 637, 770, 1342
HphI GGTGA 6 cut(s) 17, 98, 314, 887, 1084, 1478
Hpy166II GTNNAC 4 cut(s) 74, 213, 254, 473
Hpy188III TCNNGA 6 cut(s) 116, 779, 817, 1135, 1337, 1371
Hpy8I GTNNAC 4 cut(s) 74, 213, 254, 473
Hpy99I CGWCG 2 cut(s) 75, 383
HpyAV CCTTC 8 cut(s) 164, 510, 580, 751, 1069, 1355, 1537, 1639
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4IV ACGT 1 cut(s) 828
HpyF3I CTNAG 5 cut(s) 188, 528, 540, 1101, 1110
HpySE526I ACGT 1 cut(s) 828
Hsp92I GRCGYC 2 cut(s) 70, 1139
Hsp92II CATG 4 cut(s) 83, 284, 302, 782
HspAI GCGC 2 cut(s) 124, 846
KflI GGGWCCC 1 cut(s) 240
KspI CCGCGG 2 cut(s) 346, 798
LmnI GCTCC 6 cut(s) 358, 639, 674, 1179, 1192, 1522
Lsp1109I GCAGC 4 cut(s) 618, 664, 930, 1067
LweI GCATC 4 cut(s) 237, 964, 1150, 1261
MabI ACCWGGT 1 cut(s) 1262
MaeII ACGT 1 cut(s) 828
MaeIII GTNAC 5 cut(s) 104, 177, 824, 1307, 1419
MbiI CCGCTC 1 cut(s) 692
MboII GAAGA 5 cut(s) 944, 1006, 1159, 1485, 1613
MfeI CAATTG 1 cut(s) 1164
MhlI GDGCHC 3 cut(s) 262, 363, 1184
MlsI TGGCCA 2 cut(s) 285, 554
MluNI TGGCCA 2 cut(s) 285, 554
MlyI GAGTC 3 cut(s) 97, 229, 587
MmeI TCCRAC 6 cut(s) 298, 561, 949, 969, 1248, 1279
Mox20I TGGCCA 2 cut(s) 285, 554
MroXI GAANNNNTTC 1 cut(s) 335
MscI TGGCCA 2 cut(s) 285, 554
MseI TTAA 3 cut(s) 855, 1128, 1443
MslI CAYNNNNRTG 3 cut(s) 303, 749, 873
Msp20I TGGCCA 2 cut(s) 285, 554
MspA1I CMGCKG 3 cut(s) 345, 797, 1649
MspI CCGG 9 cut(s) 62, 217, 244, 431, 462, 477, 637, 770, 1342
MspR9I CCNGG 6 cut(s) 62, 217, 244, 356, 477, 1264
MunI CAATTG 1 cut(s) 1164
MvaI CCWGG 2 cut(s) 356, 1264
MvnI CGCG 7 cut(s) 117, 124, 126, 264, 345, 644, 797
NciI CCSGG 4 cut(s) 62, 217, 244, 477
NcoI CCATGG 2 cut(s) 280, 298
NlaIII CATG 4 cut(s) 83, 284, 302, 782
NlaIV GGNNCC 4 cut(s) 241, 242, 591, 1181
NmeAIII GCCGAG 2 cut(s) 589, 1613
NmuCI GTSAC 2 cut(s) 104, 824
NruI TCGCGA 1 cut(s) 117
NspI RCATGY 1 cut(s) 83
NspV TTCGAA 1 cut(s) 1088
OliI CACNNNNGTG 1 cut(s) 873
PaeR7I CTCGAG 1 cut(s) 687
PagI TCATGA 1 cut(s) 778
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PdmI GAANNNNTTC 1 cut(s) 335
PfeI GAWTC 4 cut(s) 8, 729, 1367, 1625
PflMI CCANNNNNTGG 1 cut(s) 292
PfoI TCCNGGA 2 cut(s) 242, 354
Ple19I CGATCG 3 cut(s) 46, 314, 816
PleI GAGTC 3 cut(s) 96, 228, 586
PpsI GAGTC 3 cut(s) 96, 228, 586
PpuMI RGGWCCY 1 cut(s) 240
PsiI TTATAA 1 cut(s) 1655
Psp124BI GAGCTC 1 cut(s) 363
Psp5II RGGWCCY 1 cut(s) 240
Psp6I CCWGG 2 cut(s) 354, 1262
PspGI CCWGG 2 cut(s) 354, 1262
PspN4I GGNNCC 4 cut(s) 241, 242, 591, 1181
PspPI GGNCC 6 cut(s) 53, 240, 295, 891, 1260, 1496
PspPPI RGGWCCY 1 cut(s) 240
PspXI VCTCGAGB 1 cut(s) 687
PvuI CGATCG 3 cut(s) 46, 314, 816
PvuII CAGCTG 1 cut(s) 1649
RruI TCGCGA 1 cut(s) 117
RsaI GTAC 1 cut(s) 510
RsaNI GTAC 1 cut(s) 509
RseI CAYNNNNRTG 3 cut(s) 303, 749, 873
SacI GAGCTC 1 cut(s) 363
SacII CCGCGG 2 cut(s) 346, 798
SalI GTCGAC 3 cut(s) 72, 211, 471
SaqAI TTAA 3 cut(s) 855, 1128, 1443
Sau96I GGNCC 6 cut(s) 53, 240, 295, 891, 1260, 1496
SchI GAGTC 3 cut(s) 97, 229, 587
ScrFI CCNGG 6 cut(s) 62, 217, 244, 356, 477, 1264
SduI GDGCHC 3 cut(s) 262, 363, 1184
SexAI ACCWGGT 1 cut(s) 1262
SfaNI GCATC 4 cut(s) 237, 964, 1150, 1261
Sfr274I CTCGAG 1 cut(s) 687
Sfr303I CCGCGG 2 cut(s) 346, 798
SfuI TTCGAA 1 cut(s) 1088
SgrBI CCGCGG 2 cut(s) 346, 798
SinI GGWCC 3 cut(s) 240, 891, 1260
SlaI CTCGAG 1 cut(s) 687
SmiMI CAYNNNNRTG 3 cut(s) 303, 749, 873
SmlI CTYRAG 2 cut(s) 687, 1607
SmoI CTYRAG 2 cut(s) 687, 1607
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 6 cut(s) 60, 215, 242, 354, 475, 1262
StyI CCWWGG 4 cut(s) 49, 280, 298, 894
TaaI ACNGT 1 cut(s) 233
TaiI ACGT 1 cut(s) 831
TaqII GACCGA 2 cut(s) 1221, 1265
TatI WGTACW 1 cut(s) 508
TauI GCSGC 7 cut(s) 186, 345, 377, 563, 791, 800, 883
TfiI GAWTC 4 cut(s) 8, 729, 1367, 1625
Tru1I TTAA 3 cut(s) 855, 1128, 1443
Tru9I TTAA 3 cut(s) 855, 1128, 1443
TscAI CASTG 2 cut(s) 1362, 1390
TseFI GTSAC 2 cut(s) 104, 824
TseI GCWGC 4 cut(s) 631, 677, 943, 1055
Tsp45I GTSAC 2 cut(s) 104, 824
TspDTI ATGAA 3 cut(s) 898, 1160, 1500
TspGWI ACGGA 3 cut(s) 211, 582, 1035
TspRI CASTG 2 cut(s) 1362, 1390
Van91I CCANNNNNTGG 1 cut(s) 292
VpaK11BI GGWCC 3 cut(s) 240, 891, 1260
XapI RAATTY 4 cut(s) 97, 395, 569, 604
XceI RCATGY 1 cut(s) 83
XhoI CTCGAG 1 cut(s) 687
XmiI GTMKAC 3 cut(s) 73, 212, 472
XmnI GAANNNNTTC 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.