pycom290g00160

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_290
OG0017887
Physical Location & Seq
Reverse (-)
127893 .. 128363
471 bp
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UTR
Exon/CDS
Intron
pycom290g00160.1

Sequence Viewer

Length: 471 bp
ATGGAGGGTCTGATCCCAATGGTTTACAAGGCAATGAAGAGGAACAGAACTCGCCGCCAATACAGCTGCCTCTCCTCCTCCTCCTCATTAGCAGCAGCAGCACCACCAGTATCAGGTCAAACTAGTAATTACAACATCGCTGACTTCTACATCGATCACAACAGTTACGTCTATGTTCCCCAACCTGCACCTTCAGCTGATCATCATCTTCCAACGATATTTAATGTTAATGGCGCGTCTTCCCCGCGGGACCAGTCGTCCTTATCCACAACTACAAACATTATTAATCATGGCGGTCGTCAGTTTCAGCACCGCCGTCACAAATCTGTTTCTCTCGGTTCTGCTTCCAACTCTATGCAAGGATTCTCTTCATCGGTGGACGCTGATGACTCCATTAATTCTCCTCCTCCAAAGCAACTTGTCCAGTTTCGGAGCCACCGAAGTTTCTTCTCATGCATCTCTGGTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.02

Weight (kDa)

9.69

Isoelectric Point (pI)

71.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44010
fragaria_vesca FvH4_1g00150
prunus_persica Prupe.7G270300_v2.0.a1
pyrus_communis pycom15g12880 pycom290g00160
rosa_roxburghii Rroxscaffold_2G00156070
rosa_rugosa Rorug01G0454000
rosa_samantha Rh2AG002100 Rh2CG002000 Rh2DG002200
rosa_wichuraiana Rw2G000180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 193
AccB1I GGYRCC 1 cut(s) 464
AccII CGCG 2 cut(s) 236, 247
AciI CCGC 5 cut(s) 55, 245, 247, 294, 313
AclWI GGATC 1 cut(s) 7
AcuI CTGAAG 1 cut(s) 177
AfiI CCNNNNNNNGG 1 cut(s) 113
AhdI GACNNNNNGTC 1 cut(s) 256
AhlI ACTAGT 1 cut(s) 122
AluBI AGCT 2 cut(s) 66, 197
AluI AGCT 2 cut(s) 66, 197
AlwI GGATC 1 cut(s) 7
ApeKI GCWGC 4 cut(s) 66, 92, 95, 98
AseI ATTAAT 2 cut(s) 285, 396
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 1 cut(s) 250
AvaII GGWCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 464
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 53, 104, 107, 110
BceAI ACGGC 1 cut(s) 300
BclI TGATCA 1 cut(s) 199
BcuI ACTAGT 1 cut(s) 122
BfaI CTAG 2 cut(s) 123, 469
BfuAI ACCTGC 1 cut(s) 193
BisI GCNGC 5 cut(s) 55, 67, 93, 96, 99
BlsI GCNGC 5 cut(s) 56, 68, 94, 97, 100
Bme18I GGWCC 1 cut(s) 250
BmeRI GACNNNNNGTC 1 cut(s) 256
BmgT120I GGNCC 1 cut(s) 250
BmiI GGNNCC 3 cut(s) 251, 434, 466
BmsI GCATC 1 cut(s) 465
BpiI GAAGAC 1 cut(s) 231
Bsa29I ATCGAT 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 245
Bsc4I CCNNNNNNNGG 1 cut(s) 113
Bse1I ACTGG 3 cut(s) 107, 253, 424
Bse3DI GCAATG 1 cut(s) 39
Bse8I GATNNNNATC 1 cut(s) 204
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 245
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 1 cut(s) 113
BseMI GCAATG 1 cut(s) 39
BseNI ACTGG 3 cut(s) 107, 253, 424
BseRI GAGGAG 6 cut(s) 64, 67, 70, 73, 393, 396
BseXI GCAGC 4 cut(s) 53, 104, 107, 110
BsgI GTGCAG 1 cut(s) 171
Bsh1236I CGCG 2 cut(s) 236, 247
Bsh1285I CGRYCG 1 cut(s) 298
BshNI GGYRCC 1 cut(s) 464
BshVI ATCGAT 1 cut(s) 153
BsiEI CGRYCG 1 cut(s) 298
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 263
Bsp143I GATC 3 cut(s) 12, 154, 199
BspACI CCGC 5 cut(s) 55, 245, 247, 294, 313
BspDI ATCGAT 1 cut(s) 153
BspFNI CGCG 2 cut(s) 236, 247
BspLI GGNNCC 3 cut(s) 251, 434, 466
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 1 cut(s) 7
BspT107I GGYRCC 1 cut(s) 464
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 3 cut(s) 107, 253, 424
BssECI CCNNGG 1 cut(s) 245
BssMI GATC 3 cut(s) 12, 154, 199
Bst4CI ACNGT 1 cut(s) 164
Bst6I CTCTTC 2 cut(s) 32, 373
BstDSI CCRYGG 1 cut(s) 245
BstFNI CGCG 2 cut(s) 236, 247
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 3 cut(s) 15, 157, 202
BstMBI GATC 3 cut(s) 12, 154, 199
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 3 cut(s) 63, 98, 194
BstUI CGCG 2 cut(s) 236, 247
BstV1I GCAGC 4 cut(s) 53, 104, 107, 110
BstV2I GAAGAC 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 153
BsuTUI ATCGAT 1 cut(s) 153
BtgI CCRYGG 1 cut(s) 245
BtgZI GCGATG 1 cut(s) 121
BveI ACCTGC 1 cut(s) 193
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 250
Cfr42I CCGCGG 1 cut(s) 248
ClaI ATCGAT 1 cut(s) 153
CseI GACGC 2 cut(s) 225, 389
CviAII CATG 2 cut(s) 290, 453
CviJI RGCY 3 cut(s) 66, 197, 435
CviKI_1 RGCY 3 cut(s) 66, 197, 435
DpnI GATC 3 cut(s) 14, 156, 201
DpnII GATC 3 cut(s) 12, 154, 199
DriI GACNNNNNGTC 1 cut(s) 256
Eam1104I CTCTTC 2 cut(s) 32, 373
Eam1105I GACNNNNNGTC 1 cut(s) 256
EarI CTCTTC 2 cut(s) 32, 373
Eco47I GGWCC 1 cut(s) 250
Eco57I CTGAAG 1 cut(s) 177
EcoT22I ATGCAT 1 cut(s) 458
FaeI CATG 2 cut(s) 293, 456
FaiI YATR 4 cut(s) 174, 291, 356, 454
FaqI GGGAC 1 cut(s) 263
FatI CATG 2 cut(s) 289, 452
FauI CCCGC 2 cut(s) 240, 252
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 5 cut(s) 55, 67, 93, 96, 99
Fsp4HI GCNGC 5 cut(s) 55, 67, 93, 96, 99
FspBI CTAG 2 cut(s) 123, 469
GlaI GCGC 1 cut(s) 235
GluI GCNGC 5 cut(s) 55, 67, 93, 96, 99
HgaI GACGC 2 cut(s) 225, 389
HhaI GCGC 1 cut(s) 236
Hin1II CATG 2 cut(s) 293, 456
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HinfI GANTC 2 cut(s) 363, 389
Hpy166II GTNNAC 2 cut(s) 25, 379
Hpy188I TCNGA 2 cut(s) 12, 432
Hpy8I GTNNAC 2 cut(s) 25, 379
HpyAV CCTTC 1 cut(s) 201
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 3 cut(s) 188, 358, 456
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 98, 194
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 2 cut(s) 293, 456
HspAI GCGC 1 cut(s) 234
Ksp22I TGATCA 1 cut(s) 199
KspI CCGCGG 1 cut(s) 248
Kzo9I GATC 3 cut(s) 12, 154, 199
LmnI GCTCC 1 cut(s) 432
LpnPI CCDG 6 cut(s) 99, 120, 198, 266, 437, 447
Lsp1109I GCAGC 4 cut(s) 53, 104, 107, 110
LweI GCATC 1 cut(s) 465
MaeI CTAG 2 cut(s) 123, 469
MaeII ACGT 1 cut(s) 168
MaeIII GTNAC 2 cut(s) 164, 317
MalI GATC 3 cut(s) 14, 156, 201
MboI GATC 3 cut(s) 12, 154, 199
MboII GAAGA 5 cut(s) 49, 200, 231, 360, 439
MluCI AATT 2 cut(s) 127, 397
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 2 cut(s) 236, 372
MnlI CCTC 8 cut(s) 33, 80, 85, 88, 91, 94, 414, 417
Mph1103I ATGCAT 1 cut(s) 458
MseI TTAA 4 cut(s) 222, 228, 285, 396
MspA1I CMGCKG 3 cut(s) 66, 197, 247
MvnI CGCG 2 cut(s) 236, 247
MwoI GCNNNNNNNGC 3 cut(s) 63, 98, 194
NdeII GATC 3 cut(s) 12, 154, 199
NlaIII CATG 2 cut(s) 293, 456
NlaIV GGNNCC 3 cut(s) 251, 434, 466
NmuCI GTSAC 1 cut(s) 317
NsiI ATGCAT 1 cut(s) 458
PcsI WCGNNNNNNNCGW 1 cut(s) 436
PfeI GAWTC 1 cut(s) 363
PkrI GCNGC 5 cut(s) 56, 68, 94, 97, 100
PleI GAGTC 1 cut(s) 383
PpsI GAGTC 1 cut(s) 383
PshBI ATTAAT 2 cut(s) 285, 396
PspN4I GGNNCC 3 cut(s) 251, 434, 466
PspPI GGNCC 1 cut(s) 250
PvuII CAGCTG 2 cut(s) 66, 197
SacII CCGCGG 1 cut(s) 248
SaqAI TTAA 4 cut(s) 222, 228, 285, 396
SatI GCNGC 5 cut(s) 55, 67, 93, 96, 99
Sau3AI GATC 3 cut(s) 12, 154, 199
Sau96I GGNCC 1 cut(s) 250
SchI GAGTC 1 cut(s) 383
SetI ASST 6 cut(s) 68, 118, 171, 187, 193, 199
SfaNI GCATC 1 cut(s) 465
Sfr303I CCGCGG 1 cut(s) 248
SgrBI CCGCGG 1 cut(s) 248
SinI GGWCC 1 cut(s) 250
SpeI ACTAGT 1 cut(s) 122
Sse9I AATT 2 cut(s) 127, 397
SsiI CCGC 5 cut(s) 55, 245, 247, 294, 313
SspMI CTAG 2 cut(s) 123, 469
TaaI ACNGT 1 cut(s) 164
TaiI ACGT 1 cut(s) 171
TaqI TCGA 1 cut(s) 153
TasI AATT 2 cut(s) 127, 397
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 1 cut(s) 363
Tru1I TTAA 4 cut(s) 222, 228, 285, 396
Tru9I TTAA 4 cut(s) 222, 228, 285, 396
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 4 cut(s) 66, 92, 95, 98
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 2 cut(s) 50, 360
VpaK11BI GGWCC 1 cut(s) 250
VspI ATTAAT 2 cut(s) 285, 396
XspI CTAG 2 cut(s) 123, 469
Zsp2I ATGCAT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.