pycom290g00250

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_290
OG0006581
Physical Location & Seq
Reverse (-)
163959 .. 164499
541 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 498 bp
ATGCATCATGATGATTTGAAAATTGTTTCTTTCTTCAGAGGTGTTGAAAGAACTTCTTCAACTGCCTTACACACGGCTGCTACTCAGGGGCATATTGATATTGTAAATCTCCTTTTGGAAACTGACTCAAATCTCGCCAAGATTGCCCGCAATAATGGTAAGACTGTGCTTCATTCAGCAGCAAGGATGGGGCACTTGGAAGTAGTCAAGTCCCTACTAAGAAAGGATCCAAGTGCTGCTTTTAGAACTGACCTGAAAGGCCAAACTGCGTTGCACATGGCTGTAAAAGGGCACAATGAGGAGATTGTGCTGGAGTTGCTGAAACCCGACCCCTCAGTTTTGACTGTGGAAGATAACAAGGGAAACAACGCATTGCATACTGCCACAAGGAAGGGGCATATTCAGGTTCTTTCTCCTTTATTTTCTTTCAATTTATTTAGTAATTTGATGAAATGTGCTACATGTTATATCAATGATTCAAGTGTCGATCAATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 PGG

Protein Analysis

166

Amino Acids

18.2

Weight (kDa)

7.89

Isoelectric Point (pI)

25.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 14 - 75 3.3e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 23 - 72 1.8e-10 Ankyrin repeats (many copies)
Ank PF00023 52 - 77 8.1e-06 Ankyrin repeat
Ank_5 PF13857 72 - 128 3.5e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 83 - 141 3.1e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 148
AclWI GGATC 2 cut(s) 221, 234
AcuI CTGAAG 1 cut(s) 19
AflIII ACRYGT 1 cut(s) 461
AgsI TTSAA 5 cut(s) 19, 47, 60, 430, 480
AlwI GGATC 2 cut(s) 221, 234
AoxI GGCC 1 cut(s) 259
ApeKI GCWGC 3 cut(s) 77, 179, 236
Asp700I GAANNNNTTC 1 cut(s) 55
BaeGI GKGCMC 2 cut(s) 195, 294
BamHI GGATCC 1 cut(s) 226
BbvI GCAGC 3 cut(s) 64, 191, 223
BccI CCATC 1 cut(s) 181
BceAI ACGGC 1 cut(s) 90
BisI GCNGC 3 cut(s) 78, 180, 237
BlsI GCNGC 3 cut(s) 79, 181, 238
BmiI GGNNCC 1 cut(s) 228
BmsI GCATC 1 cut(s) 13
BpmI CTGGAG 1 cut(s) 332
Bse3DI GCAATG 1 cut(s) 371
BseGI GGATG 1 cut(s) 192
BseMI GCAATG 1 cut(s) 371
BseMII CTCAG 2 cut(s) 98, 348
BseRI GAGGAG 1 cut(s) 314
BseSI GKGCMC 2 cut(s) 195, 294
BseXI GCAGC 3 cut(s) 64, 191, 223
BshFI GGCC 1 cut(s) 261
BslFI GGGAC 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 196
BsnI GGCC 1 cut(s) 261
Bsp1286I GDGCHC 2 cut(s) 195, 294
Bsp143I GATC 2 cut(s) 226, 487
BspACI CCGC 1 cut(s) 148
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 2 cut(s) 97, 347
BspHI TCATGA 1 cut(s) 7
BspLI GGNNCC 1 cut(s) 228
BspPI GGATC 2 cut(s) 221, 234
BsrDI GCAATG 1 cut(s) 371
BssMI GATC 2 cut(s) 226, 487
Bst4CI ACNGT 2 cut(s) 166, 346
BstC8I GCNNGC 1 cut(s) 148
BstDEI CTNAG 3 cut(s) 84, 218, 334
BstF5I GGATG 1 cut(s) 192
BstKTI GATC 2 cut(s) 229, 490
BstMBI GATC 2 cut(s) 226, 487
BstMWI GCNNNNNNNGC 2 cut(s) 143, 316
BstNSI RCATGY 1 cut(s) 465
BstSLI GKGCMC 2 cut(s) 195, 294
BstV1I GCAGC 3 cut(s) 64, 191, 223
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BsuRI GGCC 1 cut(s) 261
BtsCI GGATG 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 148
CciI TCATGA 1 cut(s) 7
CviAII CATG 3 cut(s) 8, 277, 462
CviJI RGCY 3 cut(s) 77, 261, 281
CviKI_1 RGCY 3 cut(s) 77, 261, 281
DdeI CTNAG 3 cut(s) 84, 218, 334
DpnI GATC 2 cut(s) 228, 489
DpnII GATC 2 cut(s) 226, 487
Eco57I CTGAAG 1 cut(s) 19
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 3 cut(s) 11, 280, 465
FaiI YATR 7 cut(s) 9, 93, 278, 378, 399, 463, 468
FalI AAGNNNNNCTT 4 cut(s) 40, 72, 223, 255
FaqI GGGAC 1 cut(s) 196
FatI CATG 3 cut(s) 7, 276, 461
FauI CCCGC 1 cut(s) 155
Fnu4HI GCNGC 3 cut(s) 78, 180, 237
FokI GGATG 1 cut(s) 199
Fsp4HI GCNGC 3 cut(s) 78, 180, 237
GluI GCNGC 3 cut(s) 78, 180, 237
GsuI CTGGAG 1 cut(s) 332
HaeIII GGCC 1 cut(s) 261
Hin1II CATG 3 cut(s) 11, 280, 465
HinfI GANTC 2 cut(s) 125, 476
Hpy188I TCNGA 1 cut(s) 38
Hpy188III TCNNGA 1 cut(s) 8
HpyAV CCTTC 1 cut(s) 385
HpyCH4III ACNGT 2 cut(s) 166, 346
HpyCH4V TGCA 3 cut(s) 4, 274, 376
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 316
HpyF3I CTNAG 3 cut(s) 84, 218, 334
Hsp92II CATG 3 cut(s) 11, 280, 465
Kzo9I GATC 2 cut(s) 226, 487
LpnPI CCDG 4 cut(s) 71, 266, 296, 389
Lsp1109I GCAGC 3 cut(s) 64, 191, 223
LweI GCATC 1 cut(s) 13
MalI GATC 2 cut(s) 228, 489
MboI GATC 2 cut(s) 226, 487
MboII GAAGA 3 cut(s) 25, 48, 362
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 2 cut(s) 195, 294
MluCI AATT 3 cut(s) 21, 430, 442
MlyI GAGTC 1 cut(s) 119
MnlI CCTC 3 cut(s) 32, 292, 343
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 55
MseI TTAA 1 cut(s) 496
MslI CAYNNNNRTG 1 cut(s) 9
MwoI GCNNNNNNNGC 2 cut(s) 143, 316
NdeII GATC 2 cut(s) 226, 487
NlaIII CATG 3 cut(s) 11, 280, 465
NlaIV GGNNCC 1 cut(s) 228
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 465
PagI TCATGA 1 cut(s) 7
PciI ACATGT 1 cut(s) 461
PdmI GAANNNNTTC 1 cut(s) 55
PfeI GAWTC 1 cut(s) 476
PkrI GCNGC 3 cut(s) 79, 181, 238
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
PscI ACATGT 1 cut(s) 461
PspN4I GGNNCC 1 cut(s) 228
PsuI RGATCY 1 cut(s) 226
RseI CAYNNNNRTG 1 cut(s) 9
SaqAI TTAA 1 cut(s) 496
SatI GCNGC 3 cut(s) 78, 180, 237
Sau3AI GATC 2 cut(s) 226, 487
SchI GAGTC 1 cut(s) 119
SduI GDGCHC 2 cut(s) 195, 294
SetI ASST 3 cut(s) 43, 255, 408
SfaNI GCATC 1 cut(s) 13
SmiMI CAYNNNNRTG 1 cut(s) 9
Sse9I AATT 3 cut(s) 21, 430, 442
SsiI CCGC 1 cut(s) 148
SspI AATATT 1 cut(s) 494
TaaI ACNGT 2 cut(s) 166, 346
TaqI TCGA 1 cut(s) 486
TasI AATT 3 cut(s) 21, 430, 442
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 1 cut(s) 496
Tru9I TTAA 1 cut(s) 496
TseI GCWGC 3 cut(s) 77, 179, 236
TspDTI ATGAA 2 cut(s) 161, 464
XceI RCATGY 1 cut(s) 465
XmnI GAANNNNTTC 1 cut(s) 55
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.