pycom461g00300

RIBOSOMAL protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000461
OG0018761
Physical Location & Seq
Forward (+)
237273 .. 238937
1665 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1665 bp
ATGGCACGAAAAGGAAATCCGATTTCGGTAAGACTTGATCTTAATCGTAGTTCAGATTCAAGTTGGTTTAGTGATTATTATTATGGTAAATTAGTGTATCAAGATGTCAATCTGAGGTCTTATTTCGGTTCCATACGTCCACCTACGAGACTTACTTTTGGCTTTCGTCTCGGTACGTGTATTATTCTACATTTTCCCAAAAGAACATTCATTCATTTCTTTCTTCCCCGTCGACCACGACGACTGAAACGACGCGAAAAATCCAGACCCGGAAAGGAGAAGGGCCGGTGGTGGGCATTTGGGAAAGTCGGGCCGATCGGGTGTCTTCATTCAAGCGGCGATACAGAAGAAGAACGAAACGAAGTGAGAGGCCGGGGGTCAGGGAAAAGAGTCGAGTCGATCAGGCTCGACGACCGGGAGAAGCAAAACGAAATTAGGATTTGGCCGAAAAAGAAGCAACGCTATGGATACCATGACCGATCACCATCGATAAAGAAGAATCTTTCTAAATCACTTCGGGTCAGCAGGGCCTTCAAGCATCCGAAATACGCCGGGGTTGTAAATGGCATAGCGTTCCTGATAGAAAATGACGACCCCTTCAGAAAAACTAAGTTATTCAAGTTCTTTTTGCCAAAGAAGTCCCGCTCCGACGGCTCGACGAGTCATCTACTTAAAAGGACCCTCCCTGCAGTGCGCCCCTCCTTCAATTATTCGGTCATGCAATACTTATTGAATACAAAGAACCAAATGCATTTCGACCCCGTCGTAGTTCTCAATCATTTCGTGGCACCGGGCGTGGCTGAACCATCTACGATGGGAGGAGCGAATGCACAGGGAAGAAGCTTAGATAAGAGAATACGTTCTCGCATCGCTTTGTTTGTAGAAAGCTCGACCAGCGATAAAAAGTGTTTGGCCGAAGCCAAAAAGAGGTTGACCCGCTTCATTCGCCAAGCGAATGATCTTCGCTTCGCGGGAACAACAAAAACCACCATCCCGCTCTTTCCTTTTTTCGGTGCTACCTTTTTCTTTCCAAGGGATGGGGTTGGGATGTATAATAACCTTTTTTTTGAGGATGCCCGGGAACAACTCCTAGGTCAATTAAGGATCAAATGTTGGAACCTCATGGGTAAGGATAAGGTAATGGAATTGATAGAGAAATTCATAGACCTAGATAGGATAGGAGAATTGATAAGGGGAATAGAGATGATGATAGAGATCATACTGAGAAACAGACAAATTCCGTGCGGGTACAACTCTTATTTGAACGAAGTGAAAAAAATGCGATCTTTGTTGTCTAATAGAACAAACACTAATACCTTAATTGAGTCGGTCAAGATCAAATCTGTTTATCAAAGTGCTTCTCCGATTGCTCAAGACATCTCTTTTCAACTGAGAAACAAAACAAGATCATTTCGTTCCATTTTTAGTAAAATAGTGAAGGATATTCCATTAGTAATGAAAAAAGGGGTTGAGGGGATCCGTATATGTTGTTCAGATCGATCAAAAGGCGCAGAAATAGCTAGAACTGAATGCGGAAAGTATGGAAAAACATCTCGTAATGTATTTAACCAGAAAATAGATTATGCTCCTGCGAAAGTATCTACTCGTTACGGAATCTTAGGTGTCAAAGTGTGGATTTCATATAGTAAGAAAAAGTCCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L16 Ribosomal_S3_C

Protein Analysis

555

Amino Acids

63.95

Weight (kDa)

10.38

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S3_C PF00189 469 - 546 1.5e-17 Ribosomal protein S3, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018761)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00090
pyrus_communis pycom461g00300
rosa_laevigata RLG00000000074
rosa_roxburghii Rroxscaffold_172G00445460 Rroxscaffold_1G00007820 Rroxscaffold_3G00250700 Rroxscaffold_7G00167100
rosa_samantha Rh1BG019100 Rh5AG095300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 787
AccB7I CCANNNNNTGG 1 cut(s) 1037
AccBSI CCGCTC 2 cut(s) 645, 997
AccI GTMKAC 1 cut(s) 232
AccII CGCG 2 cut(s) 255, 971
AciI CCGC 7 cut(s) 336, 643, 937, 971, 995, 1245, 1533
AclWI GGATC 3 cut(s) 1112, 1471, 1484
AcoI YGGCCR 2 cut(s) 443, 912
AcsI RAATTY 2 cut(s) 1157, 1236
AcuI CTGAAG 1 cut(s) 583
AfaI GTAC 2 cut(s) 175, 1250
AfiI CCNNNNNNNGG 4 cut(s) 292, 927, 1010, 1037
AflIII ACRYGT 1 cut(s) 176
AgsI TTSAA 8 cut(s) 60, 333, 535, 619, 706, 733, 1264, 1388
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AluBI AGCT 3 cut(s) 843, 888, 1520
AluI AGCT 3 cut(s) 843, 888, 1520
Alw26I GTCTC 2 cut(s) 142, 173
AlwI GGATC 3 cut(s) 1112, 1471, 1484
Ama87I CYCGRG 1 cut(s) 1077
AoxI GGCC 6 cut(s) 283, 311, 370, 443, 528, 912
ApoI RAATTY 2 cut(s) 1157, 1236
Asp700I GAANNNNTTC 1 cut(s) 859
AspA2I CCTAGG 1 cut(s) 1090
AspLEI GCGC 2 cut(s) 696, 1511
AspS9I GGNCC 4 cut(s) 283, 311, 528, 678
AsuC2I CCSGG 7 cut(s) 270, 374, 416, 553, 792, 1078, 1079
AsuHPI GGTGA 1 cut(s) 474
AvaI CYCGRG 1 cut(s) 1077
AvaII GGWCC 1 cut(s) 678
AvrII CCTAGG 1 cut(s) 1090
BamHI GGATCC 1 cut(s) 1476
BanI GGYRCC 1 cut(s) 787
BbsI GAAGAC 1 cut(s) 317
BccI CCATC 5 cut(s) 493, 808, 814, 998, 1031
BceAI ACGGC 1 cut(s) 667
BciVI GTATCC 1 cut(s) 461
BcnI CCSGG 7 cut(s) 270, 374, 416, 553, 792, 1078, 1079
BcoDI GTCTC 2 cut(s) 142, 173
BfaI CTAG 3 cut(s) 1091, 1169, 1521
BfmI CTRYAG 1 cut(s) 687
BfuI GTATCC 1 cut(s) 461
BisI GCNGC 1 cut(s) 337
BlnI CCTAGG 1 cut(s) 1090
BlsI GCNGC 1 cut(s) 338
Bme1390I CCNGG 7 cut(s) 270, 374, 416, 553, 792, 1078, 1079
Bme18I GGWCC 1 cut(s) 678
BmeT110I CYCGRG 1 cut(s) 1077
BmgT120I GGNCC 4 cut(s) 283, 311, 528, 678
BmiI GGNNCC 5 cut(s) 130, 680, 789, 1118, 1478
BmrFI CCNGG 7 cut(s) 270, 374, 416, 553, 792, 1078, 1079
BmsI GCATC 3 cut(s) 547, 876, 1063
BpiI GAAGAC 1 cut(s) 317
BpuEI CTTGAG 1 cut(s) 1356
BpuMI CCSGG 7 cut(s) 270, 374, 416, 553, 792, 1078, 1079
Bsa29I ATCGAT 2 cut(s) 488, 1498
BsaAI YACGTR 1 cut(s) 177
BsaBI GATNNNNATC 4 cut(s) 42, 108, 484, 1214
BsaJI CCNNGG 5 cut(s) 373, 552, 1031, 1077, 1090
Bsc4I CCNNNNNNNGG 4 cut(s) 292, 927, 1010, 1037
Bse118I RCCGGY 1 cut(s) 285
Bse8I GATNNNNATC 4 cut(s) 42, 108, 484, 1214
BseCI ATCGAT 2 cut(s) 488, 1498
BseDI CCNNGG 5 cut(s) 373, 552, 1031, 1077, 1090
BseGI GGATG 5 cut(s) 538, 990, 1042, 1053, 1078
BseJI GATNNNNATC 4 cut(s) 42, 108, 484, 1214
BseLI CCNNNNNNNGG 4 cut(s) 292, 927, 1010, 1037
BseMII CTCAG 3 cut(s) 104, 1214, 1382
BseRI GAGGAG 1 cut(s) 834
Bsh1236I CGCG 2 cut(s) 255, 971
Bsh1285I CGRYCG 2 cut(s) 318, 415
BshFI GGCC 6 cut(s) 285, 313, 372, 445, 530, 914
BshNI GGYRCC 1 cut(s) 787
BshVI ATCGAT 2 cut(s) 488, 1498
BsiEI CGRYCG 2 cut(s) 318, 415
BsiHKCI CYCGRG 1 cut(s) 1077
BsiSI CCGG 7 cut(s) 270, 286, 373, 415, 552, 791, 1078
BslFI GGGAC 1 cut(s) 625
BslI CCNNNNNNNGG 4 cut(s) 292, 927, 1010, 1037
BsmAI GTCTC 2 cut(s) 142, 173
BsmBI CGTCTC 1 cut(s) 173
BsmFI GGGAC 1 cut(s) 625
BsmI GAATGC 2 cut(s) 832, 1535
BsnI GGCC 6 cut(s) 285, 313, 372, 445, 530, 914
BsoBI CYCGRG 1 cut(s) 1077
BspACI CCGC 7 cut(s) 336, 643, 937, 971, 995, 1245, 1533
BspANI GGCC 6 cut(s) 285, 313, 372, 445, 530, 914
BspCNI CTCAG 3 cut(s) 105, 1215, 1383
BspDI ATCGAT 2 cut(s) 488, 1498
BspFNI CGCG 2 cut(s) 255, 971
BspLI GGNNCC 5 cut(s) 130, 680, 789, 1118, 1478
BspMAI CTGCAG 1 cut(s) 691
BspPI GGATC 3 cut(s) 1112, 1471, 1484
BspT107I GGYRCC 1 cut(s) 787
BsrBI CCGCTC 2 cut(s) 645, 997
BsrFI RCCGGY 1 cut(s) 285
BssAI RCCGGY 1 cut(s) 285
BssECI CCNNGG 5 cut(s) 373, 552, 1031, 1077, 1090
BssT1I CCWWGG 2 cut(s) 1031, 1090
BstBAI YACGTR 1 cut(s) 177
BstDEI CTNAG 6 cut(s) 113, 609, 844, 1223, 1391, 1618
BstF5I GGATG 5 cut(s) 538, 990, 1042, 1053, 1078
BstFNI CGCG 2 cut(s) 255, 971
BstHHI GCGC 2 cut(s) 696, 1511
BstMAI GTCTC 2 cut(s) 142, 173
BstMCI CGRYCG 2 cut(s) 318, 415
BstMWI GCNNNNNNNGC 4 cut(s) 651, 894, 945, 1517
BstSCI CCNGG 7 cut(s) 268, 372, 414, 551, 790, 1076, 1077
BstSFI CTRYAG 1 cut(s) 687
BstUI CGCG 2 cut(s) 255, 971
BstV2I GAAGAC 1 cut(s) 317
BstX2I RGATCY 1 cut(s) 1476
BstYI RGATCY 1 cut(s) 1476
Bsu15I ATCGAT 2 cut(s) 488, 1498
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 6 cut(s) 285, 313, 372, 445, 530, 914
BsuTUI ATCGAT 2 cut(s) 488, 1498
BtgZI GCGATG 1 cut(s) 853
BtsCI GGATG 5 cut(s) 538, 990, 1042, 1053, 1078
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 1 cut(s) 696
CfoI GCGC 2 cut(s) 696, 1511
Cfr10I RCCGGY 1 cut(s) 285
Cfr13I GGNCC 4 cut(s) 283, 311, 528, 678
Cfr9I CCCGGG 1 cut(s) 1077
ClaI ATCGAT 2 cut(s) 488, 1498
CseI GACGC 1 cut(s) 261
Csp6I GTAC 2 cut(s) 174, 1249
CviAII CATG 3 cut(s) 473, 718, 1123
CviQI GTAC 2 cut(s) 174, 1249
DdeI CTNAG 6 cut(s) 113, 609, 844, 1223, 1391, 1618
EaeI YGGCCR 2 cut(s) 443, 912
Eco130I CCWWGG 2 cut(s) 1031, 1090
Eco47I GGWCC 1 cut(s) 678
Eco57I CTGAAG 1 cut(s) 583
Eco88I CYCGRG 1 cut(s) 1077
EcoO109I RGGNCCY 2 cut(s) 528, 678
EcoT14I CCWWGG 2 cut(s) 1031, 1090
EcoT22I ATGCAT 1 cut(s) 753
ErhI CCWWGG 2 cut(s) 1031, 1090
Esp3I CGTCTC 1 cut(s) 173
FaeI CATG 3 cut(s) 476, 721, 1126
FaqI GGGAC 1 cut(s) 625
FatI CATG 3 cut(s) 472, 717, 1122
FauI CCCGC 5 cut(s) 650, 944, 964, 1002, 1238
FauNDI CATATG 1 cut(s) 1661
FblI GTMKAC 1 cut(s) 232
Fnu4HI GCNGC 1 cut(s) 337
FokI GGATG 5 cut(s) 525, 977, 1049, 1060, 1085
Fsp4HI GCNGC 1 cut(s) 337
FspBI CTAG 3 cut(s) 1091, 1169, 1521
GlaI GCGC 2 cut(s) 695, 1510
GluI GCNGC 1 cut(s) 337
HaeIII GGCC 6 cut(s) 285, 313, 372, 445, 530, 914
HapII CCGG 7 cut(s) 270, 286, 373, 415, 552, 791, 1078
HgaI GACGC 1 cut(s) 261
HhaI GCGC 2 cut(s) 696, 1511
Hin1II CATG 3 cut(s) 476, 721, 1126
Hin6I GCGC 2 cut(s) 694, 1509
HinP1I GCGC 2 cut(s) 694, 1509
HincII GTYRAC 2 cut(s) 233, 933
HindII GTYRAC 2 cut(s) 233, 933
HindIII AAGCTT 1 cut(s) 841
HinfI GANTC 7 cut(s) 56, 390, 395, 499, 661, 1325, 1614
HpaII CCGG 7 cut(s) 270, 286, 373, 415, 552, 791, 1078
HphI GGTGA 1 cut(s) 474
Hpy166II GTNNAC 3 cut(s) 140, 233, 933
Hpy188I TCNGA 8 cut(s) 21, 55, 114, 543, 602, 649, 1365, 1495
Hpy188III TCNNGA 5 cut(s) 101, 264, 577, 1333, 1373
Hpy8I GTNNAC 3 cut(s) 140, 233, 933
Hpy99I CGWCG 7 cut(s) 234, 243, 255, 413, 653, 661, 767
HpyAV CCTTC 5 cut(s) 274, 541, 607, 712, 1432
HpyCH4IV ACGT 3 cut(s) 136, 176, 859
HpyCH4V TGCA 4 cut(s) 689, 721, 751, 830
HpyF10VI GCNNNNNNNGC 4 cut(s) 651, 894, 945, 1517
HpyF3I CTNAG 6 cut(s) 113, 609, 844, 1223, 1391, 1618
HpySE526I ACGT 3 cut(s) 136, 176, 859
Hsp92II CATG 3 cut(s) 476, 721, 1126
HspAI GCGC 2 cut(s) 694, 1509
LmnI GCTCC 3 cut(s) 650, 821, 1591
LweI GCATC 3 cut(s) 547, 876, 1063
MaeI CTAG 3 cut(s) 1091, 1169, 1521
MaeII ACGT 3 cut(s) 136, 176, 859
MaeIII GTNAC 1 cut(s) 1607
MbiI CCGCTC 2 cut(s) 645, 997
MboII GAAGA 7 cut(s) 215, 317, 359, 362, 508, 849, 953
MflI RGATCY 1 cut(s) 1476
MluCI AATT 9 cut(s) 89, 432, 706, 1097, 1145, 1157, 1184, 1236, 1320
MlyI GAGTC 4 cut(s) 399, 404, 670, 1334
MmeI TCCRAC 2 cut(s) 672, 1094
MnlI CCTC 9 cut(s) 108, 362, 692, 709, 812, 921, 1063, 1130, 1465
Mph1103I ATGCAT 1 cut(s) 753
MroXI GAANNNNTTC 1 cut(s) 859
MseI TTAA 5 cut(s) 42, 672, 1100, 1319, 1566
MspI CCGG 7 cut(s) 270, 286, 373, 415, 552, 791, 1078
MspR9I CCNGG 7 cut(s) 270, 374, 416, 553, 792, 1078, 1079
Mva1269I GAATGC 2 cut(s) 832, 1535
MvnI CGCG 2 cut(s) 255, 971
MwoI GCNNNNNNNGC 4 cut(s) 651, 894, 945, 1517
NciI CCSGG 7 cut(s) 270, 374, 416, 553, 792, 1078, 1079
NdeI CATATG 1 cut(s) 1661
NlaIII CATG 3 cut(s) 476, 721, 1126
NlaIV GGNNCC 5 cut(s) 130, 680, 789, 1118, 1478
NsiI ATGCAT 1 cut(s) 753
PcsI WCGNNNNNNNCGW 3 cut(s) 238, 247, 762
PctI GAATGC 2 cut(s) 832, 1535
PdmI GAANNNNTTC 1 cut(s) 859
PfeI GAWTC 3 cut(s) 56, 499, 1614
PflFI GACNNNGTC 1 cut(s) 761
PflMI CCANNNNNTGG 1 cut(s) 1037
PkrI GCNGC 1 cut(s) 338
Ple19I CGATCG 1 cut(s) 318
PleI GAGTC 4 cut(s) 398, 403, 669, 1333
PpsI GAGTC 4 cut(s) 398, 403, 669, 1333
Ppu21I YACGTR 1 cut(s) 177
PpuMI RGGWCCY 1 cut(s) 678
Psp5II RGGWCCY 1 cut(s) 678
PspN4I GGNNCC 5 cut(s) 130, 680, 789, 1118, 1478
PspPI GGNCC 4 cut(s) 283, 311, 528, 678
PspPPI RGGWCCY 1 cut(s) 678
PsrI GAACNNNNNNTAC 2 cut(s) 1474, 1506
PstI CTGCAG 1 cut(s) 691
PsuI RGATCY 1 cut(s) 1476
PsyI GACNNNGTC 1 cut(s) 761
PvuI CGATCG 1 cut(s) 318
RsaI GTAC 2 cut(s) 175, 1250
RsaNI GTAC 2 cut(s) 174, 1249
SalI GTCGAC 1 cut(s) 231
SaqAI TTAA 5 cut(s) 42, 672, 1100, 1319, 1566
SatI GCNGC 1 cut(s) 337
Sau96I GGNCC 4 cut(s) 283, 311, 528, 678
SchI GAGTC 4 cut(s) 399, 404, 670, 1334
ScrFI CCNGG 7 cut(s) 270, 374, 416, 553, 792, 1078, 1079
SfaNI GCATC 3 cut(s) 547, 876, 1063
SfcI CTRYAG 1 cut(s) 687
SinI GGWCC 1 cut(s) 678
SmaI CCCGGG 1 cut(s) 1079
SmlI CTYRAG 1 cut(s) 1371
SmoI CTYRAG 1 cut(s) 1371
Sse9I AATT 9 cut(s) 89, 432, 706, 1097, 1145, 1157, 1184, 1236, 1320
SsiI CCGC 7 cut(s) 336, 643, 937, 971, 995, 1245, 1533
SspMI CTAG 3 cut(s) 1091, 1169, 1521
StyD4I CCNGG 7 cut(s) 268, 372, 414, 551, 790, 1076, 1077
StyI CCWWGG 2 cut(s) 1031, 1090
TaiI ACGT 3 cut(s) 139, 179, 862
TaqI TCGA 9 cut(s) 232, 393, 398, 408, 488, 656, 756, 890, 1498
TaqII GACCGA 3 cut(s) 492, 703, 1318
TasI AATT 9 cut(s) 89, 432, 706, 1097, 1145, 1157, 1184, 1236, 1320
TauI GCSGC 1 cut(s) 339
TfiI GAWTC 3 cut(s) 56, 499, 1614
Tru1I TTAA 5 cut(s) 42, 672, 1100, 1319, 1566
Tru9I TTAA 5 cut(s) 42, 672, 1100, 1319, 1566
TscAI CASTG 1 cut(s) 696
TspDTI ATGAA 7 cut(s) 199, 203, 317, 931, 1150, 1472, 1629
TspGWI ACGGA 3 cut(s) 1230, 1469, 1626
TspMI CCCGGG 1 cut(s) 1077
TspRI CASTG 1 cut(s) 696
Tth111I GACNNNGTC 1 cut(s) 761
Van91I CCANNNNNTGG 1 cut(s) 1037
VpaK11BI GGWCC 1 cut(s) 678
XapI RAATTY 2 cut(s) 1157, 1236
XmaI CCCGGG 1 cut(s) 1077
XmaJI CCTAGG 1 cut(s) 1090
XmiI GTMKAC 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 859
XspI CTAG 3 cut(s) 1091, 1169, 1521
Zsp2I ATGCAT 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.