RLG00000000074

RIBOSOMAL protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Unknown
OG0018761
Physical Location & Seq
Reverse (-)
411470 .. 417933
6464 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000074

Sequence Viewer

Length: 1320 bp
ATGTCAGTCAGGCCATTCCAGGAGACAGCAAGCGGGCTGGGACCGAAGCTCGCTATGCGGAATGAACGGGAAAGTGGAACCGGAAAGGAGTGGTGGGCTTTTGGGAAAGTCGGACCGATCAGGTGTCTTCATTCAAGCGACGATACAGAAGAAGAACGAAACGAAGTGAGAGGCCGGGGGGCAGGGAAAAGAGTCGAGTCGATCAGGCTCGACGACCGGGAGAAGCAAAACGAAATTCGGATTTGGCCGAAAAAGAAGCAACGCTATGGATACCATGACCGATCACCATCGAGAAAGAATAATCTTTTTAAATCACTTCGGGTCAGCAGGGCTTTCAAGCATCCGAAATACGCCGGGGTTGTAAATGACATAGCGTTCCTGATAGAAAATGACGACTCCTTCAGAAAAACAAAGTTATTAAAGTTCTTTTTGCCAAAGAAATCCGGCCCGACGAGTCATCTACTTAAAAGGACCCTCCCTGCAGTGCGCCCCTCTTTGAATTATTCGGTCATGCAATACTTATTGAATACAAAGAACCAAATTAATTTAGACCCCGTCGTAGTTCTCAATCATTTCGTGGCACCAAGCGTGTCTGAACCATCTACGATGGGGGGAGCGAATGCACAGGGAAGAAGCTTAGATAAGAGAATACGTTCTCGCATCGCTTTTTTTGTAGAAAGCTCGACCAGCGAGAAAAAGTCTTTGGCCGAAGCCAAAAAGAGGTTGACCCACTTCATTCGCTTGGCGAATGATCTTCGCTTCGCGGGAACAACAAAAACCACCATCTCGCTCTTTCCTTTTTTCGGTGCTACCTTTTTCTTTCCAAGGGATGGGGTTGGGATGTATAATAACCTTTTTTTTGAAGATGCCCGGGAACAACTCCTAGGTCAATTAAGAATCAAATGTTGGAACCTCATGGGTAAGGATAAGAGCTGGTCGTCTTTCACCATTGAAGCAGCGCGTCGGGCTACAACCGGAGAATTCCATCGTGCTATGAGCCGAAGAAATCGTAAGATATGGGTAAGAGTTTTCGCAGATCTCCCTATTACCGGGAAACCTACAGAAGTAAGAATGGGAAGAGGAAAAGGAAATCCTACGGGTTGGATTGCTCGTGTGTCCACGGCATTTGAAATGGATGGTGCGTACGACCCCGATACGTTACGGTTTTTATCCGCCGGCCCGGGAGCTACGAGCAAGGCAGCTCTGCTCCGGAAAGAAAAAAAGCCGGCAGCTTTGCCTGCCGCGCTTCGGTCAGTAGGATGGATAGCAAAGCCGCCTTCGGCCCTTCGCTTAGTAAGCCCCTCTTCGGGCCGCGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L16 Ribosomal_S3_C

Protein Analysis

440

Amino Acids

49.51

Weight (kDa)

10.6

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L16 PF00252 313 - 374 1.6e-14 Ribosomal protein L16p/L10e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018761)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00090
pyrus_communis pycom461g00300
rosa_laevigata RLG00000000074
rosa_roxburghii Rroxscaffold_172G00445460 Rroxscaffold_1G00007820 Rroxscaffold_3G00250700 Rroxscaffold_7G00167100
rosa_samantha Rh1BG019100 Rh5AG095300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 580
AccB7I CCANNNNNTGG 1 cut(s) 830
AccII CGCG 4 cut(s) 764, 961, 1244, 1314
AccIII TCCGGA 1 cut(s) 1209
AciI CCGC 7 cut(s) 33, 58, 764, 1173, 1242, 1274, 1312
AcoI YGGCCR 2 cut(s) 245, 705
AcsI RAATTY 2 cut(s) 234, 980
AcuI CTGAAG 1 cut(s) 385
AfaI GTAC 1 cut(s) 1145
AfiI CCNNNNNNNGG 7 cut(s) 720, 803, 830, 1049, 1248, 1306, 1307
AgsI TTSAA 7 cut(s) 135, 337, 499, 526, 863, 953, 1130
AjnI CCWGG 1 cut(s) 18
AjuI GAANNNNNNNTTGG 4 cut(s) 686, 718, 889, 921
AluBI AGCT 7 cut(s) 49, 636, 681, 933, 1187, 1202, 1232
AluI AGCT 7 cut(s) 49, 636, 681, 933, 1187, 1202, 1232
Alw26I GTCTC 1 cut(s) 17
Ama87I CYCGRG 2 cut(s) 870, 1180
Aor13HI TCCGGA 1 cut(s) 1209
AoxI GGCC 8 cut(s) 11, 172, 245, 445, 705, 1177, 1281, 1309
ApeKI GCWGC 3 cut(s) 956, 1199, 1229
ApoI RAATTY 2 cut(s) 234, 980
AseI ATTAAT 1 cut(s) 543
Asp700I GAANNNNTTC 1 cut(s) 652
AspA2I CCTAGG 1 cut(s) 883
AspLEI GCGC 3 cut(s) 489, 961, 1246
AspS9I GGNCC 7 cut(s) 41, 113, 446, 471, 1178, 1282, 1309
AsuC2I CCSGG 8 cut(s) 176, 218, 355, 871, 872, 1051, 1181, 1182
AsuHPI GGTGA 2 cut(s) 276, 937
AvaI CYCGRG 2 cut(s) 870, 1180
AvaII GGWCC 3 cut(s) 41, 113, 471
AvrII CCTAGG 1 cut(s) 883
BanI GGYRCC 1 cut(s) 580
BauI CACGAG 1 cut(s) 1110
BbsI GAAGAC 1 cut(s) 119
BbvI GCAGC 3 cut(s) 968, 1211, 1241
BccI CCATC 8 cut(s) 295, 601, 607, 791, 824, 993, 1130, 1254
BceAI ACGGC 1 cut(s) 1137
BciT130I CCWGG 1 cut(s) 20
BciVI GTATCC 1 cut(s) 263
BcnI CCSGG 8 cut(s) 176, 218, 355, 871, 872, 1051, 1181, 1182
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 1 cut(s) 884
BfmI CTRYAG 2 cut(s) 480, 1059
BfuI GTATCC 1 cut(s) 263
BglII AGATCT 1 cut(s) 1036
BisI GCNGC 6 cut(s) 957, 1200, 1230, 1242, 1274, 1312
BlnI CCTAGG 1 cut(s) 883
BlsI GCNGC 6 cut(s) 958, 1201, 1231, 1243, 1275, 1313
Bme1390I CCNGG 9 cut(s) 20, 176, 218, 355, 871, 872, 1051, 1181, 1182
Bme18I GGWCC 3 cut(s) 41, 113, 471
BmeT110I CYCGRG 2 cut(s) 870, 1180
BmgT120I GGNCC 7 cut(s) 41, 113, 446, 471, 1178, 1282, 1309
BmiI GGNNCC 5 cut(s) 42, 79, 473, 582, 911
BmrFI CCNGG 9 cut(s) 20, 176, 218, 355, 871, 872, 1051, 1181, 1182
BmsI GCATC 3 cut(s) 349, 669, 856
BpiI GAAGAC 1 cut(s) 119
BpuMI CCSGG 8 cut(s) 176, 218, 355, 871, 872, 1051, 1181, 1182
BsaBI GATNNNNATC 1 cut(s) 286
BsaJI CCNNGG 7 cut(s) 175, 354, 824, 870, 883, 1119, 1180
BsaWI WCCGGW 3 cut(s) 80, 974, 1209
Bsc4I CCNNNNNNNGG 7 cut(s) 720, 803, 830, 1049, 1248, 1306, 1307
Bse118I RCCGGY 2 cut(s) 1175, 1225
Bse8I GATNNNNATC 1 cut(s) 286
BseAI TCCGGA 1 cut(s) 1209
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 7 cut(s) 175, 354, 824, 870, 883, 1119, 1180
BseGI GGATG 5 cut(s) 340, 835, 846, 1141, 1265
BseJI GATNNNNATC 1 cut(s) 286
BseLI CCNNNNNNNGG 7 cut(s) 720, 803, 830, 1049, 1248, 1306, 1307
BseXI GCAGC 3 cut(s) 968, 1211, 1241
BseYI CCCAGC 1 cut(s) 37
Bsh1236I CGCG 4 cut(s) 764, 961, 1244, 1314
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 8 cut(s) 13, 174, 247, 447, 707, 1179, 1283, 1311
BshNI GGYRCC 1 cut(s) 580
BsiEI CGRYCG 1 cut(s) 217
BsiHKCI CYCGRG 2 cut(s) 870, 1180
BsiWI CGTACG 1 cut(s) 1143
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 7 cut(s) 720, 803, 830, 1049, 1248, 1306, 1307
BsmAI GTCTC 1 cut(s) 17
BsmFI GGGAC 1 cut(s) 54
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 8 cut(s) 13, 174, 247, 447, 707, 1179, 1283, 1311
BsoBI CYCGRG 2 cut(s) 870, 1180
Bsp13I TCCGGA 1 cut(s) 1209
Bsp143I GATC 5 cut(s) 117, 201, 281, 751, 1036
BspACI CCGC 7 cut(s) 33, 58, 764, 1173, 1242, 1274, 1312
BspANI GGCC 8 cut(s) 13, 174, 247, 447, 707, 1179, 1283, 1311
BspEI TCCGGA 1 cut(s) 1209
BspFNI CGCG 4 cut(s) 764, 961, 1244, 1314
BspLI GGNNCC 5 cut(s) 42, 79, 473, 582, 911
BspMAI CTGCAG 1 cut(s) 484
BspT107I GGYRCC 1 cut(s) 580
BsrFI RCCGGY 2 cut(s) 1175, 1225
BssAI RCCGGY 2 cut(s) 1175, 1225
BssECI CCNNGG 7 cut(s) 175, 354, 824, 870, 883, 1119, 1180
BssMI GATC 5 cut(s) 117, 201, 281, 751, 1036
BssSI CACGAG 1 cut(s) 1110
BssT1I CCWWGG 2 cut(s) 824, 883
Bst2BI CACGAG 1 cut(s) 1110
Bst2UI CCWGG 1 cut(s) 20
Bst4CI ACNGT 1 cut(s) 1164
Bst6I CTCTTC 2 cut(s) 1072, 1309
BstC8I GCNNGC 6 cut(s) 31, 35, 51, 1177, 1227, 1239
BstDEI CTNAG 2 cut(s) 637, 1291
BstDSI CCRYGG 1 cut(s) 1119
BstF5I GGATG 5 cut(s) 340, 835, 846, 1141, 1265
BstFNI CGCG 4 cut(s) 764, 961, 1244, 1314
BstHHI GCGC 3 cut(s) 489, 961, 1246
BstKTI GATC 5 cut(s) 120, 204, 284, 754, 1039
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 5 cut(s) 117, 201, 281, 751, 1036
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 6 cut(s) 55, 687, 965, 1238, 1243, 1296
BstNI CCWGG 1 cut(s) 20
BstSCI CCNGG 9 cut(s) 18, 174, 216, 353, 869, 870, 1049, 1179, 1180
BstSFI CTRYAG 2 cut(s) 480, 1059
BstUI CGCG 4 cut(s) 764, 961, 1244, 1314
BstV1I GCAGC 3 cut(s) 968, 1211, 1241
BstV2I GAAGAC 1 cut(s) 119
BstX2I RGATCY 1 cut(s) 1036
BstYI RGATCY 1 cut(s) 1036
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 8 cut(s) 13, 174, 247, 447, 707, 1179, 1283, 1311
BtgI CCRYGG 1 cut(s) 1119
BtgZI GCGATG 1 cut(s) 646
BtsCI GGATG 5 cut(s) 340, 835, 846, 1141, 1265
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 1 cut(s) 489
Cac8I GCNNGC 6 cut(s) 31, 35, 51, 1177, 1227, 1239
CfoI GCGC 3 cut(s) 489, 961, 1246
Cfr10I RCCGGY 2 cut(s) 1175, 1225
Cfr13I GGNCC 7 cut(s) 41, 113, 446, 471, 1178, 1282, 1309
Cfr9I CCCGGG 2 cut(s) 870, 1180
CpoI CGGWCCG 1 cut(s) 113
CseI GACGC 2 cut(s) 950, 1303
Csp6I GTAC 1 cut(s) 1144
CspCI CAANNNNNGTGG 2 cut(s) 1108, 1143
CspI CGGWCCG 1 cut(s) 113
CviAII CATG 3 cut(s) 275, 511, 916
CviQI GTAC 1 cut(s) 1144
DdeI CTNAG 2 cut(s) 637, 1291
DpnI GATC 5 cut(s) 119, 203, 283, 753, 1038
DpnII GATC 5 cut(s) 117, 201, 281, 751, 1036
DraI TTTAAA 1 cut(s) 310
EaeI YGGCCR 2 cut(s) 245, 705
Eam1104I CTCTTC 2 cut(s) 1072, 1309
EarI CTCTTC 2 cut(s) 1072, 1309
EciI GGCGGA 1 cut(s) 1162
Eco130I CCWWGG 2 cut(s) 824, 883
Eco47I GGWCC 3 cut(s) 41, 113, 471
Eco57I CTGAAG 1 cut(s) 385
Eco88I CYCGRG 2 cut(s) 870, 1180
EcoO109I RGGNCCY 1 cut(s) 471
EcoRI GAATTC 1 cut(s) 980
EcoRII CCWGG 1 cut(s) 18
EcoT14I CCWWGG 2 cut(s) 824, 883
ErhI CCWWGG 2 cut(s) 824, 883
FaeI CATG 3 cut(s) 278, 514, 919
FaiI YATR 9 cut(s) 56, 267, 276, 371, 512, 846, 917, 995, 1018
FalI AAGNNNNNCTT 2 cut(s) 1288, 1320
FaqI GGGAC 1 cut(s) 54
FatI CATG 3 cut(s) 274, 510, 915
FauI CCCGC 2 cut(s) 26, 757
Fnu4HI GCNGC 6 cut(s) 957, 1200, 1230, 1242, 1274, 1312
FokI GGATG 5 cut(s) 327, 842, 853, 1148, 1272
Fsp4HI GCNGC 6 cut(s) 957, 1200, 1230, 1242, 1274, 1312
FspBI CTAG 1 cut(s) 884
GlaI GCGC 3 cut(s) 488, 960, 1245
GluI GCNGC 6 cut(s) 957, 1200, 1230, 1242, 1274, 1312
GsaI CCCAGC 1 cut(s) 41
HaeIII GGCC 8 cut(s) 13, 174, 247, 447, 707, 1179, 1283, 1311
HgaI GACGC 2 cut(s) 950, 1303
HhaI GCGC 3 cut(s) 489, 961, 1246
Hin1II CATG 3 cut(s) 278, 514, 919
Hin6I GCGC 3 cut(s) 487, 959, 1244
HinP1I GCGC 3 cut(s) 487, 959, 1244
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HindIII AAGCTT 1 cut(s) 634
HinfI GANTC 5 cut(s) 192, 197, 395, 454, 897
HphI GGTGA 2 cut(s) 276, 937
Hpy166II GTNNAC 2 cut(s) 726, 1119
Hpy188I TCNGA 5 cut(s) 113, 240, 345, 404, 595
Hpy188III TCNNGA 3 cut(s) 291, 379, 1210
Hpy8I GTNNAC 2 cut(s) 726, 1119
Hpy99I CGWCG 5 cut(s) 143, 215, 454, 560, 966
HpyAV CCTTC 3 cut(s) 409, 1287, 1295
HpyCH4III ACNGT 1 cut(s) 1164
HpyCH4IV ACGT 2 cut(s) 652, 1157
HpyCH4V TGCA 3 cut(s) 482, 514, 623
HpyF10VI GCNNNNNNNGC 6 cut(s) 55, 687, 965, 1238, 1243, 1296
HpyF3I CTNAG 2 cut(s) 637, 1291
HpySE526I ACGT 2 cut(s) 652, 1157
Hsp92II CATG 3 cut(s) 278, 514, 919
HspAI GCGC 3 cut(s) 487, 959, 1244
Kpn2I TCCGGA 1 cut(s) 1209
KroI GCCGGC 2 cut(s) 1175, 1225
KroNI GCCGGC 2 cut(s) 1177, 1227
Kzo9I GATC 5 cut(s) 117, 201, 281, 751, 1036
LmnI GCTCC 3 cut(s) 614, 1184, 1212
Lsp1109I GCAGC 3 cut(s) 968, 1211, 1241
LweI GCATC 3 cut(s) 349, 669, 856
MaeI CTAG 1 cut(s) 884
MaeII ACGT 2 cut(s) 652, 1157
MaeIII GTNAC 1 cut(s) 1158
MalI GATC 5 cut(s) 119, 203, 283, 753, 1038
MboI GATC 5 cut(s) 117, 201, 281, 751, 1036
MboII GAAGA 9 cut(s) 119, 161, 164, 642, 746, 875, 1014, 1089, 1296
MflI RGATCY 1 cut(s) 1036
MluCI AATT 6 cut(s) 234, 499, 540, 544, 890, 980
MlyI GAGTC 4 cut(s) 201, 206, 389, 463
MmeI TCCRAC 3 cut(s) 91, 887, 1082
MnlI CCTC 7 cut(s) 164, 485, 502, 714, 923, 1073, 1312
MroI TCCGGA 1 cut(s) 1209
MroNI GCCGGC 2 cut(s) 1175, 1225
MroXI GAANNNNTTC 1 cut(s) 652
MseI TTAA 5 cut(s) 309, 419, 465, 543, 893
MspR9I CCNGG 9 cut(s) 20, 176, 218, 355, 871, 872, 1051, 1181, 1182
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 1 cut(s) 20
MvnI CGCG 4 cut(s) 764, 961, 1244, 1314
MwoI GCNNNNNNNGC 6 cut(s) 55, 687, 965, 1238, 1243, 1296
NaeI GCCGGC 2 cut(s) 1177, 1227
NciI CCSGG 8 cut(s) 176, 218, 355, 871, 872, 1051, 1181, 1182
NdeII GATC 5 cut(s) 117, 201, 281, 751, 1036
NgoMIV GCCGGC 2 cut(s) 1175, 1225
NlaIII CATG 3 cut(s) 278, 514, 919
NlaIV GGNNCC 5 cut(s) 42, 79, 473, 582, 911
PctI GAATGC 1 cut(s) 625
PdiI GCCGGC 2 cut(s) 1177, 1227
PdmI GAANNNNTTC 1 cut(s) 652
PfeI GAWTC 1 cut(s) 897
Pfl23II CGTACG 1 cut(s) 1143
PflFI GACNNNGTC 1 cut(s) 554
PflMI CCANNNNNTGG 1 cut(s) 830
PfoI TCCNGGA 1 cut(s) 18
PkrI GCNGC 6 cut(s) 958, 1201, 1231, 1243, 1275, 1313
PleI GAGTC 4 cut(s) 200, 205, 389, 462
PpsI GAGTC 4 cut(s) 200, 205, 389, 462
PpuMI RGGWCCY 1 cut(s) 471
PshBI ATTAAT 1 cut(s) 543
Psp5II RGGWCCY 1 cut(s) 471
Psp6I CCWGG 1 cut(s) 18
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 1 cut(s) 18
PspLI CGTACG 1 cut(s) 1143
PspN4I GGNNCC 5 cut(s) 42, 79, 473, 582, 911
PspPI GGNCC 7 cut(s) 41, 113, 446, 471, 1178, 1282, 1309
PspPPI RGGWCCY 1 cut(s) 471
PstI CTGCAG 1 cut(s) 484
PsuI RGATCY 1 cut(s) 1036
PsyI GACNNNGTC 1 cut(s) 554
RsaI GTAC 1 cut(s) 1145
RsaNI GTAC 1 cut(s) 1144
Rsr2I CGGWCCG 1 cut(s) 113
RsrII CGGWCCG 1 cut(s) 113
SaqAI TTAA 5 cut(s) 309, 419, 465, 543, 893
SatI GCNGC 6 cut(s) 957, 1200, 1230, 1242, 1274, 1312
Sau3AI GATC 5 cut(s) 117, 201, 281, 751, 1036
Sau96I GGNCC 7 cut(s) 41, 113, 446, 471, 1178, 1282, 1309
SchI GAGTC 4 cut(s) 201, 206, 389, 463
ScrFI CCNGG 9 cut(s) 20, 176, 218, 355, 871, 872, 1051, 1181, 1182
SfaNI GCATC 3 cut(s) 349, 669, 856
SfcI CTRYAG 2 cut(s) 480, 1059
SinI GGWCC 3 cut(s) 41, 113, 471
SmaI CCCGGG 2 cut(s) 872, 1182
Sse9I AATT 6 cut(s) 234, 499, 540, 544, 890, 980
SsiI CCGC 7 cut(s) 33, 58, 764, 1173, 1242, 1274, 1312
SspMI CTAG 1 cut(s) 884
StyD4I CCNGG 9 cut(s) 18, 174, 216, 353, 869, 870, 1049, 1179, 1180
StyI CCWWGG 2 cut(s) 824, 883
TaaI ACNGT 1 cut(s) 1164
TaiI ACGT 2 cut(s) 655, 1160
TaqI TCGA 5 cut(s) 195, 200, 210, 290, 683
TaqII GACCGA 5 cut(s) 58, 130, 294, 496, 1239
TasI AATT 6 cut(s) 234, 499, 540, 544, 890, 980
TauI GCSGC 3 cut(s) 1244, 1276, 1314
TfiI GAWTC 1 cut(s) 897
Tru1I TTAA 5 cut(s) 309, 419, 465, 543, 893
Tru9I TTAA 5 cut(s) 309, 419, 465, 543, 893
TscAI CASTG 1 cut(s) 489
TseI GCWGC 3 cut(s) 956, 1199, 1229
TspDTI ATGAA 3 cut(s) 78, 119, 724
TspMI CCCGGG 2 cut(s) 870, 1180
TspRI CASTG 1 cut(s) 489
Tth111I GACNNNGTC 1 cut(s) 554
Van91I CCANNNNNTGG 1 cut(s) 830
VpaK11BI GGWCC 3 cut(s) 41, 113, 471
VspI ATTAAT 1 cut(s) 543
XapI RAATTY 2 cut(s) 234, 980
XmaI CCCGGG 2 cut(s) 870, 1180
XmaJI CCTAGG 1 cut(s) 883
XmnI GAANNNNTTC 1 cut(s) 652
XspI CTAG 1 cut(s) 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.