pycom461g00320

Ribosomal protein S14p/S29e

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000461
OG0014120
Physical Location & Seq
Forward (+)
253101 .. 254126
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1026 bp
ATGGGGAAAATGACCCAGATCTTGAATCAACTAGTAGATCATCAACACGGGGTCCCAATTCATTCGGCGACCGCGAAGTCACCGAATGAATTGCCGAGCTGGGGCAGCCCGCGCGTGACCCATACCAATCCTACACTCCCTTCTTCTATTGTTTCTTTGTCACTTCTTGGAAATCACGAAGAGGATTCCCGGACTGGACTGAGCGGGAGGGGAGCTGCTGTCGGTATTGGAAACGTGTTCAGTTCTTTGATCCATTCCGTGGCCCGAAATCCATCATTGGCTAAACAATCATTTGGTTATGCCATTTTGGGCTTTGCTCTAACCGAAGCTATTGCATCGTTTGCCCCAATGATGGCCTTTTTGATCTCATCTGTATTCCGATCGAGGTTGAACTTTCATTCTTACAACTATCTTTTTGAAGCAGATAGTTCACAGTGCTCATTCTATAGATACTGTAAAAGCCAACTCATGTTTCCACTCAATTTTCATTACGAAGATGTATTACGTCAGGATCCGTTGCTCAAACTGAATCACGCTAACGTTATGGAAGTTCCTGGATCGTGTGAAATAAGAGTTTTACCAAAGGCACCCTATGATTTCATAATAAAAAATGGAAAATTGGCTATGGAGATTCCGCGCGGTCAGAAATTCATACAGACAAAAAGGGGTTCGACAGGAAAGTCGTTTCGATCCAATCCATTCTTGGGGTCAAATAAAGACAAAGGATATGCCAGTGACCTAGCACGACAAAGCACTCTCCGAGGGCATGGAATGTCTAATTTTTCGGTCAGAATCTCGACAGTAATGTCTCTGTTAGATTCCCCGGTCGAAATACGGGAAAACTTCATTCAATTCTCGATGGAAACGGAGTTTTGCGAATTCTCCCCGGAACTGGAAGATCATTTCGAGATCTTCGAGCATATTCGAGGGTTCAATGTGACTATTGTCACTTCGGCCAACACGCAAGATGAGACTTTACCACCGTGGAGCGGCTTTTTGCAAAAAGATGAGGGGGAAACTCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S14

Protein Analysis

342

Amino Acids

38.01

Weight (kDa)

6.64

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_C PF00137 65 - 122 3.5e-10 ATP synthase subunit C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014120)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07725 ATMG00210
fragaria_vesca FvH4_3g34030
pyrus_communis pycom461g00320
rosa_chinensis RchiOBHm_Chr5g0061711 RchiOBHm_Chr5g0065471
rosa_laevigata RLG00000035495
rosa_multiflora Rmu_sc0005048.1_g000010
rosa_roxburghii Rroxscaffold_1G00019070 Rroxscaffold_5G00382560
rosa_rugosa Rorug05G0342300 Rorug05G0342400
rosa_samantha Rh5AG404100 Rh5BG416800 Rh5CG441300 Rh5DG431100
rosa_wichuraiana Rw5G037970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 76, 679, 805
AccB1I GGYRCC 1 cut(s) 586
AccB7I CCANNNNNTGG 1 cut(s) 259
AccBSI CCGCTC 2 cut(s) 204, 990
AccII CGCG 5 cut(s) 74, 112, 114, 637, 639
AciI CCGC 6 cut(s) 72, 110, 204, 635, 639, 990
AclI AACGTT 1 cut(s) 540
AclWI GGATC 5 cut(s) 244, 506, 519, 565, 684
AcoI YGGCCR 1 cut(s) 954
AcsI RAATTY 2 cut(s) 647, 878
AfiI CCNNNNNNNGG 6 cut(s) 101, 259, 352, 704, 892, 989
AflIII ACRYGT 1 cut(s) 234
AgsI TTSAA 5 cut(s) 25, 391, 419, 851, 934
AhlI ACTAGT 1 cut(s) 31
AjnI CCWGG 1 cut(s) 553
AjuI GAANNNNNNNTTGG 2 cut(s) 456, 488
AluBI AGCT 3 cut(s) 99, 215, 329
AluI AGCT 3 cut(s) 99, 215, 329
Alw21I GWGCWC 1 cut(s) 440
Alw26I GTCTC 2 cut(s) 813, 965
AlwI GGATC 5 cut(s) 244, 506, 519, 565, 684
AoxI GGCC 3 cut(s) 261, 354, 954
ApeKI GCWGC 2 cut(s) 105, 215
ApoI RAATTY 2 cut(s) 647, 878
AspLEI GCGC 2 cut(s) 114, 639
AspS9I GGNCC 2 cut(s) 52, 262
AsuC2I CCSGG 3 cut(s) 190, 824, 887
AsuHPI GGTGA 1 cut(s) 72
AvaII GGWCC 1 cut(s) 52
BamHI GGATCC 1 cut(s) 511
BanI GGYRCC 1 cut(s) 586
BarI GAAGNNNNNNTAC 2 cut(s) 486, 518
Bbv12I GWGCWC 1 cut(s) 440
BbvI GCAGC 2 cut(s) 117, 202
BccI CCATC 3 cut(s) 280, 346, 853
BcgI CGANNNNNNTGC 4 cut(s) 73, 107, 314, 348
BciT130I CCWGG 1 cut(s) 555
BcnI CCSGG 3 cut(s) 190, 824, 887
BcoDI GTCTC 2 cut(s) 813, 965
BcuI ACTAGT 1 cut(s) 31
BfaI CTAG 2 cut(s) 32, 740
BfmI CTRYAG 1 cut(s) 445
BglII AGATCT 2 cut(s) 18, 909
BisI GCNGC 3 cut(s) 106, 216, 991
BlsI GCNGC 3 cut(s) 107, 217, 992
Bme1390I CCNGG 4 cut(s) 190, 555, 824, 887
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 52, 262
BmiI GGNNCC 4 cut(s) 53, 54, 513, 588
BmrFI CCNGG 4 cut(s) 190, 555, 824, 887
BmsI GCATC 1 cut(s) 344
BoxI GACNNNNGTC 1 cut(s) 944
BpuMI CCSGG 3 cut(s) 190, 824, 887
BsaJI CCNNGG 5 cut(s) 258, 760, 822, 885, 983
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 6 cut(s) 101, 259, 352, 704, 892, 989
Bse1I ACTGG 3 cut(s) 199, 732, 897
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 5 cut(s) 258, 760, 822, 885, 983
BseLI CCNNNNNNNGG 6 cut(s) 101, 259, 352, 704, 892, 989
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 3 cut(s) 199, 732, 897
BseXI GCAGC 2 cut(s) 117, 202
BseYI CCCAGC 1 cut(s) 99
Bsh1236I CGCG 5 cut(s) 74, 112, 114, 637, 639
Bsh1285I CGRYCG 3 cut(s) 72, 383, 828
BshFI GGCC 3 cut(s) 263, 356, 956
BshNI GGYRCC 1 cut(s) 586
BsiEI CGRYCG 3 cut(s) 72, 383, 828
BsiHKAI GWGCWC 1 cut(s) 440
BsiSI CCGG 3 cut(s) 190, 824, 887
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 6 cut(s) 101, 259, 352, 704, 892, 989
BsmAI GTCTC 2 cut(s) 813, 965
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 3 cut(s) 263, 356, 956
Bsp1286I GDGCHC 1 cut(s) 440
BspACI CCGC 6 cut(s) 72, 110, 204, 635, 639, 990
BspANI GGCC 3 cut(s) 263, 356, 956
BspCNI CTCAG 1 cut(s) 192
BspFNI CGCG 5 cut(s) 74, 112, 114, 637, 639
BspLI GGNNCC 4 cut(s) 53, 54, 513, 588
BspPI GGATC 5 cut(s) 244, 506, 519, 565, 684
BspT107I GGYRCC 1 cut(s) 586
BsrBI CCGCTC 2 cut(s) 204, 990
BsrI ACTGG 3 cut(s) 199, 732, 897
BssECI CCNNGG 5 cut(s) 258, 760, 822, 885, 983
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 4 cut(s) 435, 455, 802, 984
Bst6I CTCTTC 1 cut(s) 174
BstAPI GCANNNNNTGC 1 cut(s) 341
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 1 cut(s) 200
BstDSI CCRYGG 2 cut(s) 258, 983
BstFNI CGCG 5 cut(s) 74, 112, 114, 637, 639
BstHHI GCGC 2 cut(s) 114, 639
BstMAI GTCTC 2 cut(s) 813, 965
BstMCI CGRYCG 3 cut(s) 72, 383, 828
BstMWI GCNNNNNNNGC 3 cut(s) 105, 111, 341
BstNI CCWGG 1 cut(s) 555
BstPAI GACNNNNGTC 1 cut(s) 944
BstSCI CCNGG 4 cut(s) 188, 553, 822, 885
BstSFI CTRYAG 1 cut(s) 445
BstUI CGCG 5 cut(s) 74, 112, 114, 637, 639
BstV1I GCAGC 2 cut(s) 117, 202
BstX2I RGATCY 3 cut(s) 18, 511, 909
BstYI RGATCY 3 cut(s) 18, 511, 909
BsuRI GGCC 3 cut(s) 263, 356, 956
BtgI CCRYGG 2 cut(s) 258, 983
BtsIMutI CAGTG 2 cut(s) 440, 739
Cac8I GCNNGC 1 cut(s) 110
CfoI GCGC 2 cut(s) 114, 639
Cfr13I GGNCC 2 cut(s) 52, 262
CviAII CATG 2 cut(s) 469, 767
DdeI CTNAG 1 cut(s) 200
DrdI GACNNNNNNGTC 3 cut(s) 76, 679, 805
DseDI GACNNNNNNGTC 3 cut(s) 76, 679, 805
EaeI YGGCCR 1 cut(s) 954
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 52
EcoO109I RGGNCCY 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 878
EcoRII CCWGG 1 cut(s) 553
FaeI CATG 2 cut(s) 472, 770
FaqI GGGAC 1 cut(s) 38
FatI CATG 2 cut(s) 468, 766
FauI CCCGC 2 cut(s) 117, 197
Fnu4HI GCNGC 3 cut(s) 106, 216, 991
Fsp4HI GCNGC 3 cut(s) 106, 216, 991
FspBI CTAG 2 cut(s) 32, 740
GlaI GCGC 2 cut(s) 113, 638
GluI GCNGC 3 cut(s) 106, 216, 991
GsaI CCCAGC 1 cut(s) 103
HaeIII GGCC 3 cut(s) 263, 356, 956
HapII CCGG 3 cut(s) 190, 824, 887
HhaI GCGC 2 cut(s) 114, 639
Hin1II CATG 2 cut(s) 472, 770
Hin6I GCGC 2 cut(s) 112, 637
HinP1I GCGC 2 cut(s) 112, 637
HinfI GANTC 6 cut(s) 25, 185, 529, 631, 792, 818
HpaII CCGG 3 cut(s) 190, 824, 887
HphI GGTGA 1 cut(s) 72
Hpy166II GTNNAC 1 cut(s) 431
Hpy188I TCNGA 4 cut(s) 380, 645, 761, 791
Hpy188III TCNNGA 6 cut(s) 22, 176, 509, 796, 856, 907
Hpy8I GTNNAC 1 cut(s) 431
HpyAV CCTTC 1 cut(s) 150
HpyCH4III ACNGT 4 cut(s) 435, 455, 802, 984
HpyCH4IV ACGT 3 cut(s) 234, 505, 540
HpyCH4V TGCA 2 cut(s) 335, 1000
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 111, 341
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 3 cut(s) 234, 505, 540
Hsp92II CATG 2 cut(s) 472, 770
HspAI GCGC 2 cut(s) 112, 637
KflI GGGWCCC 1 cut(s) 52
LmnI GCTCC 2 cut(s) 212, 987
Lsp1109I GCAGC 2 cut(s) 117, 202
LweI GCATC 1 cut(s) 344
MaeI CTAG 2 cut(s) 32, 740
MaeII ACGT 3 cut(s) 234, 505, 540
MaeIII GTNAC 6 cut(s) 78, 115, 159, 734, 937, 946
MbiI CCGCTC 2 cut(s) 204, 990
MboII GAAGA 5 cut(s) 135, 191, 506, 904, 908
MflI RGATCY 3 cut(s) 18, 511, 909
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 8 cut(s) 57, 89, 481, 617, 647, 778, 851, 878
MnlI CCTC 6 cut(s) 175, 201, 378, 755, 920, 1003
MspI CCGG 3 cut(s) 190, 824, 887
MspR9I CCNGG 4 cut(s) 190, 555, 824, 887
MvaI CCWGG 1 cut(s) 555
MvnI CGCG 5 cut(s) 74, 112, 114, 637, 639
MwoI GCNNNNNNNGC 3 cut(s) 105, 111, 341
NciI CCSGG 3 cut(s) 190, 824, 887
NlaIII CATG 2 cut(s) 472, 770
NlaIV GGNNCC 4 cut(s) 53, 54, 513, 588
NmeAIII GCCGAG 1 cut(s) 120
NmuCI GTSAC 6 cut(s) 78, 115, 159, 734, 937, 946
PcsI WCGNNNNNNNCGW 2 cut(s) 71, 912
PfeI GAWTC 6 cut(s) 25, 185, 529, 631, 792, 818
PflMI CCANNNNNTGG 1 cut(s) 259
PfoI TCCNGGA 2 cut(s) 188, 553
PkrI GCNGC 3 cut(s) 107, 217, 992
Ple19I CGATCG 1 cut(s) 383
PpuMI RGGWCCY 1 cut(s) 52
PshAI GACNNNNGTC 1 cut(s) 944
Psp1406I AACGTT 1 cut(s) 540
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 553
PspFI CCCAGC 1 cut(s) 99
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 4 cut(s) 53, 54, 513, 588
PspPI GGNCC 2 cut(s) 52, 262
PspPPI RGGWCCY 1 cut(s) 52
PsuI RGATCY 3 cut(s) 18, 511, 909
PvuI CGATCG 1 cut(s) 383
SatI GCNGC 3 cut(s) 106, 216, 991
Sau96I GGNCC 2 cut(s) 52, 262
ScrFI CCNGG 4 cut(s) 190, 555, 824, 887
SduI GDGCHC 1 cut(s) 440
SetI ASST 8 cut(s) 101, 217, 237, 331, 389, 508, 543, 741
SfaNI GCATC 1 cut(s) 344
SfcI CTRYAG 1 cut(s) 445
SinI GGWCC 1 cut(s) 52
SpeI ACTAGT 1 cut(s) 31
Sse9I AATT 8 cut(s) 57, 89, 481, 617, 647, 778, 851, 878
SsiI CCGC 6 cut(s) 72, 110, 204, 635, 639, 990
SspMI CTAG 2 cut(s) 32, 740
StyD4I CCNGG 4 cut(s) 188, 553, 822, 885
TaaI ACNGT 4 cut(s) 435, 455, 802, 984
TaiI ACGT 3 cut(s) 237, 508, 543
TaqI TCGA 9 cut(s) 383, 671, 688, 797, 828, 857, 906, 915, 925
TaqII GACCGA 1 cut(s) 775
TasI AATT 8 cut(s) 57, 89, 481, 617, 647, 778, 851, 878
TauI GCSGC 1 cut(s) 993
TfiI GAWTC 6 cut(s) 25, 185, 529, 631, 792, 818
TscAI CASTG 2 cut(s) 440, 739
TseFI GTSAC 6 cut(s) 78, 115, 159, 734, 937, 946
TseI GCWGC 2 cut(s) 105, 215
Tsp45I GTSAC 6 cut(s) 78, 115, 159, 734, 937, 946
TspDTI ATGAA 7 cut(s) 50, 102, 386, 476, 589, 640, 835
TspGWI ACGGA 4 cut(s) 247, 504, 881, 1011
TspRI CASTG 2 cut(s) 440, 739
Van91I CCANNNNNTGG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 52
XapI RAATTY 2 cut(s) 647, 878
XcmI CCANNNNNNNNNTGG 1 cut(s) 700
XspI CTAG 2 cut(s) 32, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.