RchiOBHm_Chr7g0178221

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0015326
Physical Location & Seq
Forward (+)
941608 .. 942456
849 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
ATGCCCATCTACCATACATTTCTCTGTCACCAACTAATCCCTATTCTGCTCTCCTCTCTCTCTCTCTCTCTGTTTCTCAATATGGGAAATGCAGCAGCTTCTTGTGTTCCTTCAAGACTCTCAAACAGTACAACAGTCAAGGTCCTATTCTTCGATGGAAGATTGGAAGTCTACATCAGACCAATCAAAGCAGCAGAGCTAATGCTAGAGCATTCAGGCCAGTTTGTGGTTGAGTCTAGCAGCCTCAGAGTAGGGAACAGAATCCATGGCCTTACAGCCGATGAAGAACTGGAAAGACGAAGATTCTACTTTCTTCTTCCCATGGATTTGCTCTACTCAGTTCTCACACATGAAGAACTCAGCGCCCTAACTTACAAGGCTTCAAGGGCACTCAAGCACAGAGGAAGCTTCAACAACTTCAGCAAGATTTTTCCGGTCCTCAGCGAGTTTTTTATGTTTCCCAACTCAGAATCAAAGCCATTGGATGGTTCTGATCATCATAGTCCGGCAAGCAATTCGTTAGTGCCATTGTTGGAGACGAGGTACTCAAAGCAAAGATCATGGAAGCCTGCATTGGAGACCATTGTTGAAACCAGTACTACATGTCCACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

204

Amino Acids

23.08

Weight (kDa)

7.81

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 28 - 197 1.1e-36 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015326)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18290
fragaria_vesca FvH4_5g17470
malus_domestica MD06G1228100.v1.1 MD14G1237300.v1.1
prunus_persica Prupe.5G234600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0178221
rosa_laevigata RLG00000005462
rosa_multiflora Rmu_co8411017.1_g000001 Rmu_sc0007855.1_g000004
rosa_roxburghii Rroxscaffold_3G00275070
rosa_rugosa Rorug06G0415100
rosa_samantha Rh7AG014200 Rh7BG013700 Rh7CG013600 Rh7DG013900
rosa_wichuraiana Rw7G001110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 226, 485
AccI GTMKAC 1 cut(s) 171
AcuI CTGAAG 1 cut(s) 403
AfaI GTAC 3 cut(s) 130, 545, 598
AfiI CCNNNNNNNGG 2 cut(s) 226, 485
AflIII ACRYGT 1 cut(s) 602
AgsI TTSAA 4 cut(s) 114, 384, 412, 590
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AluBI AGCT 3 cut(s) 98, 199, 408
AluI AGCT 3 cut(s) 98, 199, 408
Alw26I GTCTC 2 cut(s) 530, 572
AoxI GGCC 2 cut(s) 217, 268
ApeKI GCWGC 4 cut(s) 92, 95, 191, 240
AspLEI GCGC 1 cut(s) 365
AspS9I GGNCC 2 cut(s) 142, 436
AsuHPI GGTGA 1 cut(s) 20
AvaII GGWCC 2 cut(s) 142, 436
BaeGI GKGCMC 1 cut(s) 391
BbvCI CCTCAGC 1 cut(s) 440
BbvI GCAGC 4 cut(s) 104, 107, 203, 252
BccI CCATC 3 cut(s) 14, 149, 479
BcgI CGANNNNNNTGC 2 cut(s) 498, 532
BclI TGATCA 1 cut(s) 493
BcoDI GTCTC 2 cut(s) 530, 572
BfaI CTAG 2 cut(s) 206, 237
BfoI RGCGCY 1 cut(s) 366
BisI GCNGC 4 cut(s) 93, 96, 192, 241
BlsI GCNGC 4 cut(s) 94, 97, 193, 242
BmcAI AGTACT 1 cut(s) 598
Bme18I GGWCC 2 cut(s) 142, 436
BmgT120I GGNCC 2 cut(s) 142, 436
Bpu10I CCTNAGC 1 cut(s) 440
BpuEI CTTGAG 1 cut(s) 377
BsaI GGTCTC 1 cut(s) 572
BsaJI CCNNGG 2 cut(s) 265, 321
BsaWI WCCGGW 1 cut(s) 433
BsaXI ACNNNNNCTCC 2 cut(s) 527, 557
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 485
Bse1I ACTGG 3 cut(s) 220, 294, 594
BseDI CCNNGG 2 cut(s) 265, 321
BseGI GGATG 1 cut(s) 490
BseLI CCNNNNNNNGG 2 cut(s) 226, 485
BseMII CTCAG 5 cut(s) 259, 351, 373, 454, 480
BseNI ACTGG 3 cut(s) 220, 294, 594
BseRI GAGGAG 1 cut(s) 43
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 4 cut(s) 104, 107, 203, 252
BshFI GGCC 2 cut(s) 219, 270
BsiSI CCGG 2 cut(s) 434, 506
BslI CCNNNNNNNGG 2 cut(s) 226, 485
BsmAI GTCTC 2 cut(s) 530, 572
BsmBI CGTCTC 1 cut(s) 530
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 2 cut(s) 219, 270
Bso31I GGTCTC 1 cut(s) 572
Bsp1286I GDGCHC 1 cut(s) 391
Bsp143I GATC 2 cut(s) 493, 557
Bsp19I CCATGG 2 cut(s) 265, 321
BspANI GGCC 2 cut(s) 219, 270
BspCNI CTCAG 5 cut(s) 258, 350, 372, 453, 479
BspTNI GGTCTC 1 cut(s) 572
BsrI ACTGG 3 cut(s) 220, 294, 594
BssECI CCNNGG 2 cut(s) 265, 321
BssMI GATC 2 cut(s) 493, 557
BssT1I CCWWGG 2 cut(s) 265, 321
Bst4CI ACNGT 2 cut(s) 128, 136
BstC8I GCNNGC 2 cut(s) 511, 570
BstDEI CTNAG 5 cut(s) 245, 337, 359, 440, 466
BstDSI CCRYGG 2 cut(s) 265, 321
BstF5I GGATG 1 cut(s) 490
BstH2I RGCGCY 1 cut(s) 366
BstHHI GCGC 1 cut(s) 365
BstKTI GATC 2 cut(s) 496, 560
BstMAI GTCTC 2 cut(s) 530, 572
BstMBI GATC 2 cut(s) 493, 557
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNSI RCATGY 1 cut(s) 606
BstSLI GKGCMC 1 cut(s) 391
BstV1I GCAGC 4 cut(s) 104, 107, 203, 252
BsuRI GGCC 2 cut(s) 219, 270
BtgI CCRYGG 2 cut(s) 265, 321
BtsCI GGATG 1 cut(s) 490
Cac8I GCNNGC 2 cut(s) 511, 570
CfoI GCGC 1 cut(s) 365
Cfr13I GGNCC 2 cut(s) 142, 436
Csp6I GTAC 3 cut(s) 129, 544, 597
CviAII CATG 5 cut(s) 266, 322, 350, 561, 603
CviQI GTAC 3 cut(s) 129, 544, 597
DdeI CTNAG 5 cut(s) 245, 337, 359, 440, 466
DpnI GATC 2 cut(s) 495, 559
DpnII GATC 2 cut(s) 493, 557
Eco130I CCWWGG 2 cut(s) 265, 321
Eco31I GGTCTC 1 cut(s) 572
Eco47I GGWCC 2 cut(s) 142, 436
Eco57I CTGAAG 1 cut(s) 403
EcoO109I RGGNCCY 1 cut(s) 142
EcoT14I CCWWGG 2 cut(s) 265, 321
ErhI CCWWGG 2 cut(s) 265, 321
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 5 cut(s) 269, 325, 353, 564, 606
FaiI YATR 9 cut(s) 15, 83, 267, 323, 351, 455, 501, 562, 604
FatI CATG 5 cut(s) 265, 321, 349, 560, 602
FbaI TGATCA 1 cut(s) 493
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 4 cut(s) 93, 96, 192, 241
FokI GGATG 1 cut(s) 497
Fsp4HI GCNGC 4 cut(s) 93, 96, 192, 241
FspBI CTAG 2 cut(s) 206, 237
GlaI GCGC 1 cut(s) 364
GluI GCNGC 4 cut(s) 93, 96, 192, 241
HaeII RGCGCY 1 cut(s) 366
HaeIII GGCC 2 cut(s) 219, 270
HapII CCGG 2 cut(s) 434, 506
HhaI GCGC 1 cut(s) 365
Hin1II CATG 5 cut(s) 269, 325, 353, 564, 606
Hin6I GCGC 1 cut(s) 363
HinP1I GCGC 1 cut(s) 363
HindIII AAGCTT 1 cut(s) 406
HinfI GANTC 5 cut(s) 117, 233, 261, 303, 470
HpaII CCGG 2 cut(s) 434, 506
HphI GGTGA 1 cut(s) 20
Hpy166II GTNNAC 2 cut(s) 172, 608
Hpy188I TCNGA 4 cut(s) 179, 248, 469, 493
Hpy188III TCNNGA 1 cut(s) 114
Hpy8I GTNNAC 2 cut(s) 172, 608
HpyAV CCTTC 1 cut(s) 120
HpyCH4III ACNGT 2 cut(s) 128, 136
HpyCH4V TGCA 2 cut(s) 92, 572
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 5 cut(s) 245, 337, 359, 440, 466
Hsp92II CATG 5 cut(s) 269, 325, 353, 564, 606
HspAI GCGC 1 cut(s) 363
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 2 cut(s) 493, 557
LpnPI CCDG 7 cut(s) 201, 233, 275, 447, 519, 582, 607
Lsp1109I GCAGC 4 cut(s) 104, 107, 203, 252
MaeI CTAG 2 cut(s) 206, 237
MaeIII GTNAC 1 cut(s) 26
MalI GATC 2 cut(s) 495, 559
MboI GATC 2 cut(s) 493, 557
MboII GAAGA 7 cut(s) 142, 171, 296, 305, 308, 312, 365
MhlI GDGCHC 1 cut(s) 391
MluCI AATT 1 cut(s) 514
MlyI GAGTC 2 cut(s) 111, 242
MmeI TCCRAC 1 cut(s) 513
MnlI CCTC 5 cut(s) 64, 254, 395, 449, 534
MspI CCGG 2 cut(s) 434, 506
Mva1269I GAATGC 1 cut(s) 211
MwoI GCNNNNNNNGC 1 cut(s) 386
NcoI CCATGG 2 cut(s) 265, 321
NdeII GATC 2 cut(s) 493, 557
NlaIII CATG 5 cut(s) 269, 325, 353, 564, 606
NmuCI GTSAC 1 cut(s) 26
NspI RCATGY 1 cut(s) 606
PciI ACATGT 1 cut(s) 602
PctI GAATGC 1 cut(s) 211
PfeI GAWTC 3 cut(s) 261, 303, 470
PflMI CCANNNNNTGG 2 cut(s) 226, 485
PkrI GCNGC 4 cut(s) 94, 97, 193, 242
PleI GAGTC 2 cut(s) 111, 241
PpsI GAGTC 2 cut(s) 111, 241
PpuMI RGGWCCY 1 cut(s) 142
PscI ACATGT 1 cut(s) 602
Psp5II RGGWCCY 1 cut(s) 142
PspPI GGNCC 2 cut(s) 142, 436
PspPPI RGGWCCY 1 cut(s) 142
RsaI GTAC 3 cut(s) 130, 545, 598
RsaNI GTAC 3 cut(s) 129, 544, 597
SatI GCNGC 4 cut(s) 93, 96, 192, 241
Sau3AI GATC 2 cut(s) 493, 557
Sau96I GGNCC 2 cut(s) 142, 436
ScaI AGTACT 1 cut(s) 598
SchI GAGTC 2 cut(s) 111, 242
SduI GDGCHC 1 cut(s) 391
SetI ASST 5 cut(s) 100, 144, 201, 410, 545
SinI GGWCC 2 cut(s) 142, 436
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
Sse9I AATT 1 cut(s) 514
SspMI CTAG 2 cut(s) 206, 237
StyI CCWWGG 2 cut(s) 265, 321
TaaI ACNGT 2 cut(s) 128, 136
TaqI TCGA 1 cut(s) 153
TasI AATT 1 cut(s) 514
TatI WGTACW 2 cut(s) 128, 596
TfiI GAWTC 3 cut(s) 261, 303, 470
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 4 cut(s) 92, 95, 191, 240
Tsp45I GTSAC 1 cut(s) 26
TspDTI ATGAA 2 cut(s) 297, 366
Van91I CCANNNNNTGG 2 cut(s) 226, 485
VpaK11BI GGWCC 2 cut(s) 142, 436
XceI RCATGY 1 cut(s) 606
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 2 cut(s) 206, 237
ZrmI AGTACT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.