RchiOBHm_Chr7g0203501

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0017164
Physical Location & Seq
Forward (+)
21189998 .. 21192093
2096 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1806 bp
ATGCAAAGGTTTCTCTTCAAGAGAATCCAAAAACCCCTCCAAAACCTTGCACTTTGTGCAAATTATATTACTACAACTATCCCAACTCATAACAATACCAATGCCACAACAATTCCTCATGTTGAAACTTCAGACTCATGGGCAAGTATCATTTTTGACCTTGCTCAATGTGGCACCAGAACTGGGCATGCCCTATTTGAGGCATCTCACATGCTCATCTCTGGTACCAAACCAAATGGGTACTTGTTTGTTCAGATGGTCAGAGCTTCAGCCAACCTGGGCTGGGATTTCTTCAGCTTGCAACTTCATAGCTACATCTTACGATCTGGGTTTTGCTCCAATGTTTTTGTCTCCACTGCTCTGATCAATTTTTATATTGGAATCGGCTCATTGAGTGATGCTCACCAGGTGTTTGTTGAAATTCCTCAACCAAGTGTAGTTTCTTGGAATTCTTTGATTTCTGGATACGTGCATTCTGGGCAGTTCTGTAAAGCGTTGAGTTTGTTTCTTCAGCTGGAAAGGTCTGAAGTTTCTGTAGATTCATATTCGTTAACTGCGGCCTTAGGTGCATGTGGCCAACTGAGCTTTCTGCTGCTTGGCAAGACAGTTCACTCAAAGACTGTGAAACTAGGTTTGGAAAATAGCACTGTTGTTTTGAATTGTTTGATTGACATGTATGGGAAATGTGGATCACTTCAGGAGGCAATCCTTGTGTTCAATAACATGATAGAAAAGGATACTATTTCGTGGAATTCCGTAATAGCGGCAAGTGCAAGAAATGGAAGCTTACAACAAGCATTCAGTTTTTTGCAGCAGATGCATAACCCTGATATAATCTCATACAATGAACTGATAAATGGCTTTTCGCGGTTTGGAGATGTAGAAGATGCTATTAAACTTCTATTCAGTATGCCAAATCCAAATTCATCTTCCTGGAATTCGATAATTACAGGATATGTAAACAGGAGTCGAGCACGAGAAGCTTTGGATTTCTTCTGCAAAATGCACTTGGAAAATGTAGAAATGGATCAATTCACGTTTTCAAGCATTTTAAGTGGAGTTGCAGGTATTTCGGCTTTAACGTGGGGCATGCTAATCCATTGTTGCACCATAAAATTTGGTTTGGCTACATCAATTGTGGTTGGTAGTTCTTTGATTGACATGTACTCCAAATGCGGGAAGATAAGCGATGCTGAAACAATCTTCCATTTACTTCCAGAGAAGAATTTGGTGACATGGAATTCAATGATCTCTGGCTTTTCTTGGAACGGTAACCCTTCAAAGATGATGCTCCTTTTCGAGCAGATGAAAATGGAGAGAGATGTCAAGCCTGATGAGATCACATTCCTTAATGTTATATCTGCATGTTTACATAATCAAATTCCATTCGAGAACGCACTTCAGTACTTTGAATCAATGATCAAAGAGTATGGGATTGAGCCGACTCTTGAGCACTGTTGTTCTATGATTCGGCTAATGGGGCAGAGAGGGGAGGTGTGGAGAGCAGAGAACATGATATTCCAGCTCGGTTTTGGGACATGCGGATCAGTTTGGAGGGCACTGCTCGGTGCTTGTGGAAAGTGTAGAGATTTGAAAGTGGCCAAAAATGCAGCTGCAAAGGTGATACAGTTAGAAGGTGAAGATGAATTTGTTTATGTTACGATGTCTAACTTGTATGCACACTATGCAAAGTGGGAAGATGTGAGGGTAGCGAGGAAGCTGATGAAGGAGAAAGGAATGAGAAAGGAAGCTGGTTATAGTTGGATTGAAAGGGAAAATGTTATCTCAAATTCTGAATTACAATAA

Protein Analysis

601

Amino Acids

67.23

Weight (kDa)

6.44

Isoelectric Point (pI)

32.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 144 - 191 1.3e-08 PPR repeat family
PPR_1 PF12854 274 - 304 3e-06 PPR repeat
PPR_2 PF13041 307 - 355 5e-11 PPR repeat family
PPR_2 PF13041 409 - 457 1.3e-09 PPR repeat family
PPR PF01535 411 - 438 2.2e-07 PPR repeat
E_motif PF20431 530 - 590 2.1e-13 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47460
fragaria_vesca FvH4_5g19050
rosa_chinensis RchiOBHm_Chr7g0203501
rosa_laevigata RLG00000003525
rosa_multiflora Rmu_sc0011994.1_g000002 Rmu_sc0028006.1_g000002
rosa_roxburghii Rroxscaffold_3G00253770
rosa_rugosa Rorug07G0076300
rosa_samantha Rh7AG205800 Rh7BG205700 Rh7CG218500 Rh7DG213000
rosa_wichuraiana Rw7G017980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1055
Acc65I GGTACC 1 cut(s) 224
AccB1I GGYRCC 2 cut(s) 173, 224
AccII CGCG 1 cut(s) 868
AciI CCGC 5 cut(s) 557, 764, 868, 1176, 1542
AclWI GGATC 3 cut(s) 697, 1035, 1552
AcoI YGGCCR 2 cut(s) 574, 1599
AcuI CTGAAG 7 cut(s) 114, 252, 277, 494, 546, 680, 1385
AdeI CACNNNGTG 2 cut(s) 56, 409
AfaI GTAC 4 cut(s) 226, 242, 1166, 1406
AfiI CCNNNNNNNGG 4 cut(s) 183, 199, 283, 1176
AflIII ACRYGT 2 cut(s) 672, 1161
AjnI CCWGG 3 cut(s) 276, 405, 932
Alw21I GWGCWC 2 cut(s) 976, 1455
Alw26I GTCTC 1 cut(s) 355
AlwI GGATC 3 cut(s) 697, 1035, 1552
AoxI GGCC 3 cut(s) 558, 574, 1599
ApeKI GCWGC 4 cut(s) 592, 811, 1610, 1613
Asp718I GGTACC 1 cut(s) 224
AsuHPI GGTGA 4 cut(s) 395, 1243, 1633, 1649
AxyI CCTNAGG 1 cut(s) 562
BaeGI GKGCMC 1 cut(s) 1561
BalI TGGCCA 2 cut(s) 576, 1601
BanI GGYRCC 2 cut(s) 173, 224
BauI CACGAG 1 cut(s) 975
Bbv12I GWGCWC 2 cut(s) 976, 1455
BbvI GCAGC 4 cut(s) 579, 823, 1600, 1622
BccI CCATC 1 cut(s) 250
BcgI CGANNNNNNTGC 2 cut(s) 1053, 1087
BciT130I CCWGG 3 cut(s) 278, 407, 934
BciVI GTATCC 2 cut(s) 458, 730
BclI TGATCA 2 cut(s) 363, 1419
BcoDI GTCTC 1 cut(s) 355
BfaI CTAG 1 cut(s) 629
BfmI CTRYAG 1 cut(s) 534
BfuAI ACCTGC 1 cut(s) 1055
BfuI GTATCC 2 cut(s) 458, 730
BisI GCNGC 6 cut(s) 558, 593, 765, 812, 1611, 1614
BlsI GCNGC 6 cut(s) 559, 594, 766, 813, 1612, 1615
BmcAI AGTACT 1 cut(s) 1406
Bme1390I CCNGG 3 cut(s) 278, 407, 934
BmiI GGNNCC 2 cut(s) 175, 226
BmrFI CCNGG 3 cut(s) 278, 407, 934
BmrI ACTGGG 1 cut(s) 192
BmsI GCATC 6 cut(s) 212, 388, 807, 877, 1180, 1278
BmuI ACTGGG 1 cut(s) 192
BplI GAGNNNNNCTC 2 cut(s) 385, 417
BpuEI CTTGAG 1 cut(s) 1469
BsaAI YACGTR 1 cut(s) 469
BsaJI CCNNGG 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 1151, 1181
Bsc4I CCNNNNNNNGG 4 cut(s) 183, 199, 283, 1176
Bse1I ACTGG 1 cut(s) 187
Bse21I CCTNAGG 1 cut(s) 562
BseBI CCWGG 3 cut(s) 278, 407, 934
BseDI CCNNGG 1 cut(s) 277
BseLI CCNNNNNNNGG 4 cut(s) 183, 199, 283, 1176
BseMII CTCAG 1 cut(s) 572
BseNI ACTGG 1 cut(s) 187
BseSI GKGCMC 1 cut(s) 1561
BseXI GCAGC 4 cut(s) 579, 823, 1600, 1622
BseYI CCCAGC 1 cut(s) 282
Bsh1236I CGCG 1 cut(s) 868
BshFI GGCC 3 cut(s) 560, 576, 1601
BshNI GGYRCC 2 cut(s) 173, 224
BsiHKAI GWGCWC 2 cut(s) 976, 1455
BslFI GGGAC 1 cut(s) 1549
BslI CCNNNNNNNGG 4 cut(s) 183, 199, 283, 1176
BsmAI GTCTC 1 cut(s) 355
BsmFI GGGAC 1 cut(s) 1549
BsmI GAATGC 2 cut(s) 472, 797
BsnI GGCC 3 cut(s) 560, 576, 1601
Bsp1286I GDGCHC 3 cut(s) 976, 1455, 1561
Bsp143I GATC 8 cut(s) 323, 363, 689, 1027, 1248, 1338, 1419, 1544
BspACI CCGC 5 cut(s) 557, 764, 868, 1176, 1542
BspANI GGCC 3 cut(s) 560, 576, 1601
BspCNI CTCAG 1 cut(s) 573
BspFNI CGCG 1 cut(s) 868
BspLI GGNNCC 2 cut(s) 175, 226
BspMI ACCTGC 1 cut(s) 1055
BspPI GGATC 3 cut(s) 697, 1035, 1552
BspT107I GGYRCC 2 cut(s) 173, 224
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 8 cut(s) 323, 363, 689, 1027, 1248, 1338, 1419, 1544
BssSI CACGAG 1 cut(s) 975
Bst2BI CACGAG 1 cut(s) 975
Bst2UI CCWGG 3 cut(s) 278, 407, 934
Bst4CI ACNGT 6 cut(s) 607, 622, 649, 1271, 1457, 1629
Bst6I CTCTTC 1 cut(s) 20
BstAPI GCANNNNNTGC 3 cut(s) 56, 817, 1685
BstBAI YACGTR 1 cut(s) 469
BstC8I GCNNGC 3 cut(s) 189, 299, 1091
BstDEI CTNAG 2 cut(s) 562, 581
BstEII GGTNACC 1 cut(s) 1271
BstENI CCTNNNNNAGG 1 cut(s) 197
BstFNI CGCG 1 cut(s) 868
BstKTI GATC 8 cut(s) 326, 366, 692, 1030, 1251, 1341, 1422, 1547
BstMAI GTCTC 1 cut(s) 355
BstMBI GATC 8 cut(s) 323, 363, 689, 1027, 1248, 1338, 1419, 1544
BstNI CCWGG 3 cut(s) 278, 407, 934
BstNSI RCATGY 8 cut(s) 191, 214, 573, 676, 1093, 1165, 1368, 1542
BstPI GGTNACC 1 cut(s) 1271
BstSCI CCNGG 3 cut(s) 276, 405, 932
BstSFI CTRYAG 1 cut(s) 534
BstSLI GKGCMC 1 cut(s) 1561
BstUI CGCG 1 cut(s) 868
BstV1I GCAGC 4 cut(s) 579, 823, 1600, 1622
Bsu36I CCTNAGG 1 cut(s) 562
BsuI GTATCC 2 cut(s) 458, 730
BsuRI GGCC 3 cut(s) 560, 576, 1601
BtgZI GCGATG 1 cut(s) 1203
BtsI GCAGTG 2 cut(s) 354, 1559
BtsIMutI CAGTG 4 cut(s) 354, 645, 1453, 1559
BveI ACCTGC 1 cut(s) 1055
Cac8I GCNNGC 3 cut(s) 189, 299, 1091
CsiI ACCWGGT 1 cut(s) 405
Csp6I GTAC 4 cut(s) 225, 241, 1165, 1405
CviQI GTAC 4 cut(s) 225, 241, 1165, 1405
DdeI CTNAG 2 cut(s) 562, 581
DpnI GATC 8 cut(s) 325, 365, 691, 1029, 1250, 1340, 1421, 1546
DpnII GATC 8 cut(s) 323, 363, 689, 1027, 1248, 1338, 1419, 1544
DraIII CACNNNGTG 2 cut(s) 56, 409
EaeI YGGCCR 2 cut(s) 574, 1599
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Eco57I CTGAAG 7 cut(s) 114, 252, 277, 494, 546, 680, 1385
Eco81I CCTNAGG 1 cut(s) 562
Eco91I GGTNACC 1 cut(s) 1271
EcoNI CCTNNNNNAGG 1 cut(s) 197
EcoO65I GGTNACC 1 cut(s) 1271
EcoRI GAATTC 4 cut(s) 448, 751, 937, 1240
EcoRII CCWGG 3 cut(s) 276, 405, 932
EcoT22I ATGCAT 1 cut(s) 822
FaqI GGGAC 1 cut(s) 1549
FauI CCCGC 1 cut(s) 1169
FbaI TGATCA 2 cut(s) 363, 1419
Fnu4HI GCNGC 6 cut(s) 558, 593, 765, 812, 1611, 1614
Fsp4HI GCNGC 6 cut(s) 558, 593, 765, 812, 1611, 1614
FspBI CTAG 1 cut(s) 629
GluI GCNGC 6 cut(s) 558, 593, 765, 812, 1611, 1614
GsaI CCCAGC 1 cut(s) 286
HaeIII GGCC 3 cut(s) 560, 576, 1601
HincII GTYRAC 1 cut(s) 552
HindII GTYRAC 1 cut(s) 552
HindIII AAGCTT 2 cut(s) 784, 981
HinfI GANTC 8 cut(s) 24, 134, 381, 539, 967, 1412, 1444, 1468
HpaI GTTAAC 1 cut(s) 552
HphI GGTGA 4 cut(s) 395, 1243, 1633, 1649
Hpy166II GTNNAC 4 cut(s) 552, 610, 961, 1370
Hpy188I TCNGA 6 cut(s) 133, 255, 263, 363, 526, 1795
Hpy188III TCNNGA 6 cut(s) 19, 462, 698, 1217, 1390, 1448
Hpy8I GTNNAC 4 cut(s) 552, 610, 961, 1370
HpyAV CCTTC 3 cut(s) 1287, 1628, 1720
HpyCH4III ACNGT 6 cut(s) 607, 622, 649, 1271, 1457, 1629
HpyCH4IV ACGT 3 cut(s) 468, 1037, 1082
HpyF3I CTNAG 2 cut(s) 562, 581
HpySE526I ACGT 3 cut(s) 468, 1037, 1082
KpnI GGTACC 1 cut(s) 228
Ksp22I TGATCA 2 cut(s) 363, 1419
KspAI GTTAAC 1 cut(s) 552
Kzo9I GATC 8 cut(s) 323, 363, 689, 1027, 1248, 1338, 1419, 1544
LmnI GCTCC 2 cut(s) 341, 1296
Lsp1109I GCAGC 4 cut(s) 579, 823, 1600, 1622
LweI GCATC 6 cut(s) 212, 388, 807, 877, 1180, 1278
MabI ACCWGGT 1 cut(s) 405
MaeI CTAG 1 cut(s) 629
MaeII ACGT 3 cut(s) 468, 1037, 1082
MaeIII GTNAC 3 cut(s) 1231, 1271, 1657
MalI GATC 8 cut(s) 325, 365, 691, 1029, 1250, 1340, 1421, 1546
MboI GATC 8 cut(s) 323, 363, 689, 1027, 1248, 1338, 1419, 1544
MfeI CAATTG 1 cut(s) 1134
MhlI GDGCHC 3 cut(s) 976, 1455, 1561
MlsI TGGCCA 2 cut(s) 576, 1601
MluNI TGGCCA 2 cut(s) 576, 1601
MlyI GAGTC 3 cut(s) 128, 976, 1438
MmeI TCCRAC 1 cut(s) 1742
Mox20I TGGCCA 2 cut(s) 576, 1601
Mph1103I ATGCAT 1 cut(s) 822
MscI TGGCCA 2 cut(s) 576, 1601
MseI TTAA 5 cut(s) 551, 894, 1052, 1079, 1350
Msp20I TGGCCA 2 cut(s) 576, 1601
MspA1I CMGCKG 2 cut(s) 514, 1613
MspR9I CCNGG 3 cut(s) 278, 407, 934
MunI CAATTG 1 cut(s) 1134
Mva1269I GAATGC 2 cut(s) 472, 797
MvaI CCWGG 3 cut(s) 278, 407, 934
MvnI CGCG 1 cut(s) 868
NdeII GATC 8 cut(s) 323, 363, 689, 1027, 1248, 1338, 1419, 1544
NlaIV GGNNCC 2 cut(s) 175, 226
NmuCI GTSAC 1 cut(s) 1231
NsiI ATGCAT 1 cut(s) 822
NspI RCATGY 8 cut(s) 191, 214, 573, 676, 1093, 1165, 1368, 1542
PaeI GCATGC 2 cut(s) 191, 1093
PciI ACATGT 2 cut(s) 672, 1161
PcsI WCGNNNNNNNCGW 1 cut(s) 1079
PctI GAATGC 2 cut(s) 472, 797
PfeI GAWTC 5 cut(s) 24, 381, 539, 1412, 1468
PfoI TCCNGGA 1 cut(s) 932
PkrI GCNGC 6 cut(s) 559, 594, 766, 813, 1612, 1615
PleI GAGTC 3 cut(s) 128, 975, 1438
PpsI GAGTC 3 cut(s) 128, 975, 1438
Ppu21I YACGTR 1 cut(s) 469
PscI ACATGT 2 cut(s) 672, 1161
Psp6I CCWGG 3 cut(s) 276, 405, 932
PspEI GGTNACC 1 cut(s) 1271
PspFI CCCAGC 1 cut(s) 282
PspGI CCWGG 3 cut(s) 276, 405, 932
PspN4I GGNNCC 2 cut(s) 175, 226
PvuII CAGCTG 2 cut(s) 514, 1613
RsaI GTAC 4 cut(s) 226, 242, 1166, 1406
RsaNI GTAC 4 cut(s) 225, 241, 1165, 1405
SaqAI TTAA 5 cut(s) 551, 894, 1052, 1079, 1350
SatI GCNGC 6 cut(s) 558, 593, 765, 812, 1611, 1614
Sau3AI GATC 8 cut(s) 323, 363, 689, 1027, 1248, 1338, 1419, 1544
ScaI AGTACT 1 cut(s) 1406
SchI GAGTC 3 cut(s) 128, 976, 1438
ScrFI CCNGG 3 cut(s) 278, 407, 934
SduI GDGCHC 3 cut(s) 976, 1455, 1561
SexAI ACCWGGT 1 cut(s) 405
SfaNI GCATC 6 cut(s) 212, 388, 807, 877, 1180, 1278
SfcI CTRYAG 1 cut(s) 534
SmlI CTYRAG 1 cut(s) 1448
SmoI CTYRAG 1 cut(s) 1448
SphI GCATGC 2 cut(s) 191, 1093
SsiI CCGC 5 cut(s) 557, 764, 868, 1176, 1542
SspMI CTAG 1 cut(s) 629
StyD4I CCNGG 3 cut(s) 276, 405, 932
TaaI ACNGT 6 cut(s) 607, 622, 649, 1271, 1457, 1629
TaiI ACGT 3 cut(s) 471, 1040, 1085
TaqI TCGA 4 cut(s) 941, 970, 1299, 1389
TatI WGTACW 2 cut(s) 1164, 1404
TauI GCSGC 2 cut(s) 560, 767
TfiI GAWTC 5 cut(s) 24, 381, 539, 1412, 1468
Tru1I TTAA 5 cut(s) 551, 894, 1052, 1079, 1350
Tru9I TTAA 5 cut(s) 551, 894, 1052, 1079, 1350
TscAI CASTG 4 cut(s) 361, 652, 1460, 1566
TseFI GTSAC 1 cut(s) 1231
TseI GCWGC 4 cut(s) 592, 811, 1610, 1613
Tsp45I GTSAC 1 cut(s) 1231
TspDTI ATGAA 7 cut(s) 296, 531, 861, 915, 1322, 1659, 1739
TspGWI ACGGA 1 cut(s) 745
TspRI CASTG 4 cut(s) 361, 652, 1460, 1566
XagI CCTNNNNNAGG 1 cut(s) 197
XceI RCATGY 8 cut(s) 191, 214, 573, 676, 1093, 1165, 1368, 1542
XcmI CCANNNNNNNNNTGG 1 cut(s) 1529
XspI CTAG 1 cut(s) 629
ZrmI AGTACT 1 cut(s) 1406
Zsp2I ATGCAT 1 cut(s) 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.