Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0017164

Physical Location & Seq

Genomic Coordinates Reverse (-)

49633776 .. 49635593

Gene Structure 1818 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003525

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGCAAAGGTTTCTCTTCAAGAGAATCCAAAAACCACTCCAAAACCTTGCACTTTGTGCAAATTATATTACCACAACTATCCCAACTCATAACAATACCAATGCCACAACAATTCCTCATGTTGAAACATCAGACTCATGGGCAGGTATCATTTTTGACCTGGCCCAATGTGGCACCAGAACTGGGCATGCCCTATTTGAGGCCTCTCACATGCTCAGCTCTGGTACCAAACCAAATGGGTACTTGTTTGTTCAGATGGTCAGAGCTTCAGCTAAACTGGGCTGGGATTTCTTCAGCTTGCAACTTCATAGCTACATTTCACGATCTGGGTTTTGCTCCAATGTTTTTGTTTCCACTGCTCTGATCAATTTTTATATCGGAATCGGCTCATTGAGTGATGCTCACAAGGTGTTTGTTGAAATTCCTCAACCAAGTGTAGTTTCTTGGAATTCTTTGATTGCTGGATACGTACATTCTGGGCATTTCCGTAAAGCGTTGAGTTTGTTTCTTCAGCTGGAAAGGTCTGAAGTTTCTGTAGATTCATATTCGTTAACTGCAGCCTTAGGTGCATGTGGCCAACTGAGCTTTCTGCTGCTTGGCAAGACAGTTCACTCAAAGACTCTGAAACTAGGTTTGCAAAATAGCACTGTTGTTTTGAATTGTTTGATTGACATGTATGGTAAATGTGGATCACTTCAGGAGGCAATCCTTGTGTTTAATAACATGATAGAAAAGGATACTATTTCGTGGAATTCCATAATAGCGGCAAGTGCAAGAAACGGAAGCTTACAACAAGCATTCAGTTTTTTGCAGCAGATGCATAACCCTGATATAATCTCATACAATGAACTAATAAATGGCTTTTCGCGGTTTGGAGACGTAGAAGTTGCTATTAAACTTCTATTCAGTATGCCAAATCCAAATTCATCTTCCTGGAATTCGATAATTACAGGATATGTAAACAGGGGTCGAGCACGAGAAGCTCTGGATTTCTTCTGCAAAATGCACTTGGAAAATGTAGAAATGGATCAATTCACGTTTTCAAGCATTTTAAGTGGAGTTGCGGGTATTTCAGCTTTAACGTGGGGCATGCTAATCCATTGTTGCACCATAAAATTTGGCTTGGCTACATCAATTGTGGTTGGTAGTTCTTTGATTGACATGTACTCCAAATGCGGGAAGGTAAGCGATGCTGAAACAATCTTCCATTTACTTCCAGAGAAGAATTTGGTGACATGGAATGCAATGATCTCTGGCTTTTCTCGGAATGGTAATCCTTCAAAGGTGATGCTTCTTTTCGAGCAGATGAAAATGGAGAGAGATGTCAAGCCTGATGAGATCACATTCCTTAATGTTATATCTGCATGTTTACATAATCAAATTCCATTTGAGAATGCACTTCAGTACTTTGAATCAATGATCAAAGAGTATGGGATTGAGCCGACTCTTGAGCATTGTTGTTCTATGATTCGGCTAATGGGGCAGAGAGGGGAGGTGTGGAGAGCAGAGAAGATGATATTCCAGCTCGGTTTTGGGGCATGCGGATCAGTTTGGAGGGCACTGCTCGGTGCTTGTGGAGAGTGTAGAGATCTGAAAGTTGCCAAAAATGCAGCTGCAAAGGTGATACAGTTAGAAGGTGATGATGAATTTGTTTATGTTACGATGTCTAACCTGTATGCCCACTATGCAAAGTGGGAAGATGTGAGGATAGTGAGGAAGCTGATGAAGGAGAAAGGAATGAGAAAGGAAGCTGGTTATAGTTGGATTGAAATGGAAAATGTTATCTCAAATTCTGAATTACAATCAAGATTGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

606

Amino Acids

67.56

Weight (kDa)

7.54

Isoelectric Point (pI)

33.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	144 - 191	2.3e-09	PPR repeat family
PPR	PF01535	147 - 175	3.2e-06	PPR repeat
PPR_2	PF13041	307 - 355	5.6e-12	PPR repeat family
PPR	PF01535	311 - 339	6.1e-06	PPR repeat
PPR_2	PF13041	409 - 457	2.2e-10	PPR repeat family
PPR	PF01535	411 - 438	2.9e-08	PPR repeat
E_motif	PF20431	530 - 590	4.9e-14	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017164)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47460
fragaria_vesca	FvH4_5g19050
rosa_chinensis	RchiOBHm_Chr7g0203501
rosa_laevigata	RLG00000003525
rosa_multiflora	Rmu_sc0011994.1_g000002 Rmu_sc0028006.1_g000002
rosa_roxburghii	Rroxscaffold_3G00253770
rosa_rugosa	Rorug07G0076300
rosa_samantha	Rh7AG205800 Rh7BG205700 Rh7CG218500 Rh7DG213000
rosa_wichuraiana	Rw7G017980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	134
Acc65I	GGTACC	1 cut(s)	224
AccB1I	GGYRCC	2 cut(s)	173, 224
AccII	CGCG	1 cut(s)	868
AciI	CCGC	5 cut(s)	764, 868, 1064, 1176, 1542
AclWI	GGATC	3 cut(s)	697, 1035, 1552
AcoI	YGGCCR	1 cut(s)	574
AcuI	CTGAAG	6 cut(s)	252, 277, 494, 546, 680, 1385
AdeI	CACNNNGTG	2 cut(s)	56, 409
AfaI	GTAC	5 cut(s)	226, 242, 471, 1166, 1406
AfiI	CCNNNNNNNGG	3 cut(s)	183, 199, 1176
AflIII	ACRYGT	2 cut(s)	672, 1161
AgsI	TTSAA	8 cut(s)	19, 125, 419, 658, 1044, 1281, 1412, 1769
AjnI	CCWGG	2 cut(s)	159, 932
AjuI	GAANNNNNNNTTGG	8 cut(s)	424, 456, 570, 602, 907, 913, 939, 945
Alw21I	GWGCWC	1 cut(s)	976
Alw26I	GTCTC	1 cut(s)	870
AlwI	GGATC	3 cut(s)	697, 1035, 1552
AoxI	GGCC	3 cut(s)	162, 201, 574
ApeKI	GCWGC	5 cut(s)	557, 592, 811, 1610, 1613
Asp718I	GGTACC	1 cut(s)	224
AspS9I	GGNCC	1 cut(s)	163
AsuHPI	GGTGA	4 cut(s)	1243, 1297, 1633, 1649
AxyI	CCTNAGG	1 cut(s)	562
BaeGI	GKGCMC	1 cut(s)	1561
BalI	TGGCCA	1 cut(s)	576
BanI	GGYRCC	2 cut(s)	173, 224
BauI	CACGAG	1 cut(s)	975
Bbv12I	GWGCWC	1 cut(s)	976
BbvI	GCAGC	5 cut(s)	569, 579, 823, 1600, 1622
BccI	CCATC	1 cut(s)	250
BciT130I	CCWGG	2 cut(s)	161, 934
BciVI	GTATCC	2 cut(s)	458, 730
BclI	TGATCA	2 cut(s)	363, 1419
BcoDI	GTCTC	1 cut(s)	870
BfaI	CTAG	1 cut(s)	629
BfmI	CTRYAG	2 cut(s)	534, 555
BfuAI	ACCTGC	1 cut(s)	134
BfuI	GTATCC	2 cut(s)	458, 730
BglII	AGATCT	1 cut(s)	1588
BisI	GCNGC	6 cut(s)	558, 593, 765, 812, 1611, 1614
BlpI	GCTNAGC	1 cut(s)	215
BlsI	GCNGC	6 cut(s)	559, 594, 766, 813, 1612, 1615
BmcAI	AGTACT	1 cut(s)	1406
Bme1390I	CCNGG	2 cut(s)	161, 934
BmgT120I	GGNCC	1 cut(s)	163
BmiI	GGNNCC	2 cut(s)	175, 226
BmrFI	CCNGG	2 cut(s)	161, 934
BmrI	ACTGGG	2 cut(s)	192, 287
BmsI	GCATC	4 cut(s)	388, 807, 1180, 1278
BmuI	ACTGGG	2 cut(s)	192, 287
BplI	GAGNNNNNCTC	2 cut(s)	385, 417
Bpu1102I	GCTNAGC	1 cut(s)	215
BpuEI	CTTGAG	1 cut(s)	1469
BsaAI	YACGTR	1 cut(s)	469
BsaXI	ACNNNNNCTCC	2 cut(s)	1151, 1181
Bsc4I	CCNNNNNNNGG	3 cut(s)	183, 199, 1176
Bse1I	ACTGG	2 cut(s)	187, 282
Bse21I	CCTNAGG	1 cut(s)	562
Bse3DI	GCAATG	1 cut(s)	1251
BseBI	CCWGG	2 cut(s)	161, 934
BseLI	CCNNNNNNNGG	3 cut(s)	183, 199, 1176
BseMI	GCAATG	1 cut(s)	1251
BseMII	CTCAG	2 cut(s)	229, 572
BseNI	ACTGG	2 cut(s)	187, 282
BseSI	GKGCMC	1 cut(s)	1561
BseXI	GCAGC	5 cut(s)	569, 579, 823, 1600, 1622
BseYI	CCCAGC	1 cut(s)	282
Bsh1236I	CGCG	1 cut(s)	868
BshFI	GGCC	3 cut(s)	164, 203, 576
BshNI	GGYRCC	2 cut(s)	173, 224
BsiHKAI	GWGCWC	1 cut(s)	976
BslI	CCNNNNNNNGG	3 cut(s)	183, 199, 1176
BsmAI	GTCTC	1 cut(s)	870
BsmBI	CGTCTC	1 cut(s)	870
BsmI	GAATGC	3 cut(s)	797, 1246, 1399
BsnI	GGCC	3 cut(s)	164, 203, 576
Bsp1286I	GDGCHC	2 cut(s)	976, 1561
Bsp143I	GATC	9 cut(s)	323, 363, 689, 1027, 1248, 1338, 1419, 1544, 1588
Bsp1720I	GCTNAGC	1 cut(s)	215
BspACI	CCGC	5 cut(s)	764, 868, 1064, 1176, 1542
BspANI	GGCC	3 cut(s)	164, 203, 576
BspCNI	CTCAG	2 cut(s)	228, 573
BspFNI	CGCG	1 cut(s)	868
BspLI	GGNNCC	2 cut(s)	175, 226
BspMAI	CTGCAG	1 cut(s)	559
BspMI	ACCTGC	1 cut(s)	134
BspPI	GGATC	3 cut(s)	697, 1035, 1552
BspT107I	GGYRCC	2 cut(s)	173, 224
BsrDI	GCAATG	1 cut(s)	1251
BsrI	ACTGG	2 cut(s)	187, 282
BssMI	GATC	9 cut(s)	323, 363, 689, 1027, 1248, 1338, 1419, 1544, 1588
BssSI	CACGAG	1 cut(s)	975
Bst2BI	CACGAG	1 cut(s)	975
Bst2UI	CCWGG	2 cut(s)	161, 934
Bst4CI	ACNGT	3 cut(s)	607, 649, 1629
Bst6I	CTCTTC	1 cut(s)	20
BstAPI	GCANNNNNTGC	2 cut(s)	56, 817
BstBAI	YACGTR	1 cut(s)	469
BstC8I	GCNNGC	4 cut(s)	189, 299, 1091, 1540
BstDEI	CTNAG	3 cut(s)	215, 562, 581
BstENI	CCTNNNNNAGG	1 cut(s)	197
BstFNI	CGCG	1 cut(s)	868
BstKTI	GATC	9 cut(s)	326, 366, 692, 1030, 1251, 1341, 1422, 1547, 1591
BstMAI	GTCTC	1 cut(s)	870
BstMBI	GATC	9 cut(s)	323, 363, 689, 1027, 1248, 1338, 1419, 1544, 1588
BstMWI	GCNNNNNNNGC	9 cut(s)	56, 566, 582, 770, 817, 980, 1480, 1607, 1685
BstNI	CCWGG	2 cut(s)	161, 934
BstNSI	RCATGY	8 cut(s)	191, 214, 573, 676, 1093, 1165, 1368, 1542
BstSCI	CCNGG	2 cut(s)	159, 932
BstSFI	CTRYAG	2 cut(s)	534, 555
BstSLI	GKGCMC	1 cut(s)	1561
BstSNI	TACGTA	1 cut(s)	469
BstUI	CGCG	1 cut(s)	868
BstV1I	GCAGC	5 cut(s)	569, 579, 823, 1600, 1622
BstX2I	RGATCY	1 cut(s)	1588
BstYI	RGATCY	1 cut(s)	1588
Bsu36I	CCTNAGG	1 cut(s)	562
BsuI	GTATCC	2 cut(s)	458, 730
BsuRI	GGCC	3 cut(s)	164, 203, 576
BtgZI	GCGATG	1 cut(s)	1203
BtsI	GCAGTG	2 cut(s)	354, 1559
BtsIMutI	CAGTG	3 cut(s)	354, 645, 1559
BveI	ACCTGC	1 cut(s)	134
Cac8I	GCNNGC	4 cut(s)	189, 299, 1091, 1540
Cfr13I	GGNCC	1 cut(s)	163
Csp6I	GTAC	5 cut(s)	225, 241, 470, 1165, 1405
CviQI	GTAC	5 cut(s)	225, 241, 470, 1165, 1405
DdeI	CTNAG	3 cut(s)	215, 562, 581
DpnI	GATC	9 cut(s)	325, 365, 691, 1029, 1250, 1340, 1421, 1546, 1590
DpnII	GATC	9 cut(s)	323, 363, 689, 1027, 1248, 1338, 1419, 1544, 1588
DraIII	CACNNNGTG	2 cut(s)	56, 409
EaeI	YGGCCR	1 cut(s)	574
Eam1104I	CTCTTC	1 cut(s)	20
EarI	CTCTTC	1 cut(s)	20
Eco105I	TACGTA	1 cut(s)	469
Eco147I	AGGCCT	1 cut(s)	203
Eco57I	CTGAAG	6 cut(s)	252, 277, 494, 546, 680, 1385
Eco81I	CCTNAGG	1 cut(s)	562
EcoNI	CCTNNNNNAGG	1 cut(s)	197
EcoRI	GAATTC	3 cut(s)	448, 751, 937
EcoRII	CCWGG	2 cut(s)	159, 932
EcoT22I	ATGCAT	1 cut(s)	822
Esp3I	CGTCTC	1 cut(s)	870
FauI	CCCGC	2 cut(s)	1057, 1169
FbaI	TGATCA	2 cut(s)	363, 1419
Fnu4HI	GCNGC	6 cut(s)	558, 593, 765, 812, 1611, 1614
Fsp4HI	GCNGC	6 cut(s)	558, 593, 765, 812, 1611, 1614
FspBI	CTAG	1 cut(s)	629
GluI	GCNGC	6 cut(s)	558, 593, 765, 812, 1611, 1614
GsaI	CCCAGC	1 cut(s)	286
HaeIII	GGCC	3 cut(s)	164, 203, 576
HincII	GTYRAC	1 cut(s)	552
HindII	GTYRAC	1 cut(s)	552
HindIII	AAGCTT	1 cut(s)	784
HinfI	GANTC	8 cut(s)	24, 134, 381, 539, 619, 1412, 1444, 1468
HpaI	GTTAAC	1 cut(s)	552
HphI	GGTGA	4 cut(s)	1243, 1297, 1633, 1649
Hpy166II	GTNNAC	4 cut(s)	552, 610, 961, 1370
Hpy188III	TCNNGA	7 cut(s)	19, 321, 698, 986, 1217, 1448, 1806
Hpy8I	GTNNAC	4 cut(s)	552, 610, 961, 1370
HpyAV	CCTTC	4 cut(s)	1174, 1287, 1628, 1720
HpyCH4III	ACNGT	3 cut(s)	607, 649, 1629
HpyCH4IV	ACGT	4 cut(s)	468, 879, 1037, 1082
HpyF10VI	GCNNNNNNNGC	9 cut(s)	56, 566, 582, 770, 817, 980, 1480, 1607, 1685
HpyF3I	CTNAG	3 cut(s)	215, 562, 581
HpySE526I	ACGT	4 cut(s)	468, 879, 1037, 1082
KpnI	GGTACC	1 cut(s)	228
Ksp22I	TGATCA	2 cut(s)	363, 1419
KspAI	GTTAAC	1 cut(s)	552
Kzo9I	GATC	9 cut(s)	323, 363, 689, 1027, 1248, 1338, 1419, 1544, 1588
LmnI	GCTCC	1 cut(s)	341
Lsp1109I	GCAGC	5 cut(s)	569, 579, 823, 1600, 1622
LweI	GCATC	4 cut(s)	388, 807, 1180, 1278
MaeI	CTAG	1 cut(s)	629
MaeII	ACGT	4 cut(s)	468, 879, 1037, 1082
MaeIII	GTNAC	2 cut(s)	1231, 1657
MalI	GATC	9 cut(s)	325, 365, 691, 1029, 1250, 1340, 1421, 1546, 1590
MboI	GATC	9 cut(s)	323, 363, 689, 1027, 1248, 1338, 1419, 1544, 1588
MboII	GAAGA	9 cut(s)	7, 283, 500, 921, 985, 1195, 1234, 1522, 1709
MfeI	CAATTG	1 cut(s)	1134
MflI	RGATCY	1 cut(s)	1588
MhlI	GDGCHC	2 cut(s)	976, 1561
MlsI	TGGCCA	1 cut(s)	576
MluNI	TGGCCA	1 cut(s)	576
MlyI	GAGTC	3 cut(s)	128, 613, 1438
MmeI	TCCRAC	1 cut(s)	1742
Mox20I	TGGCCA	1 cut(s)	576
Mph1103I	ATGCAT	1 cut(s)	822
MscI	TGGCCA	1 cut(s)	576
MseI	TTAA	6 cut(s)	551, 717, 894, 1052, 1079, 1350
Msp20I	TGGCCA	1 cut(s)	576
MspA1I	CMGCKG	2 cut(s)	514, 1613
MspR9I	CCNGG	2 cut(s)	161, 934
MunI	CAATTG	1 cut(s)	1134
Mva1269I	GAATGC	3 cut(s)	797, 1246, 1399
MvaI	CCWGG	2 cut(s)	161, 934
MvnI	CGCG	1 cut(s)	868
MwoI	GCNNNNNNNGC	9 cut(s)	56, 566, 582, 770, 817, 980, 1480, 1607, 1685
NdeII	GATC	9 cut(s)	323, 363, 689, 1027, 1248, 1338, 1419, 1544, 1588
NlaIV	GGNNCC	2 cut(s)	175, 226
NmuCI	GTSAC	1 cut(s)	1231
NsiI	ATGCAT	1 cut(s)	822
NspI	RCATGY	8 cut(s)	191, 214, 573, 676, 1093, 1165, 1368, 1542
PaeI	GCATGC	3 cut(s)	191, 1093, 1542
PceI	AGGCCT	1 cut(s)	203
PciI	ACATGT	2 cut(s)	672, 1161
PctI	GAATGC	3 cut(s)	797, 1246, 1399
PfeI	GAWTC	5 cut(s)	24, 381, 539, 1412, 1468
PfoI	TCCNGGA	1 cut(s)	932
PkrI	GCNGC	6 cut(s)	559, 594, 766, 813, 1612, 1615
PleI	GAGTC	3 cut(s)	128, 613, 1438
PpsI	GAGTC	3 cut(s)	128, 613, 1438
Ppu21I	YACGTR	1 cut(s)	469
PscI	ACATGT	2 cut(s)	672, 1161
Psp6I	CCWGG	2 cut(s)	159, 932
PspFI	CCCAGC	1 cut(s)	282
PspGI	CCWGG	2 cut(s)	159, 932
PspN4I	GGNNCC	2 cut(s)	175, 226
PspPI	GGNCC	1 cut(s)	163
PstI	CTGCAG	1 cut(s)	559
PsuI	RGATCY	1 cut(s)	1588
PvuII	CAGCTG	2 cut(s)	514, 1613
RsaI	GTAC	5 cut(s)	226, 242, 471, 1166, 1406
RsaNI	GTAC	5 cut(s)	225, 241, 470, 1165, 1405
SaqAI	TTAA	6 cut(s)	551, 717, 894, 1052, 1079, 1350
SatI	GCNGC	6 cut(s)	558, 593, 765, 812, 1611, 1614
Sau3AI	GATC	9 cut(s)	323, 363, 689, 1027, 1248, 1338, 1419, 1544, 1588
Sau96I	GGNCC	1 cut(s)	163
ScaI	AGTACT	1 cut(s)	1406
SchI	GAGTC	3 cut(s)	128, 613, 1438
ScrFI	CCNGG	2 cut(s)	161, 934
SduI	GDGCHC	2 cut(s)	976, 1561
SfaNI	GCATC	4 cut(s)	388, 807, 1180, 1278
SfcI	CTRYAG	2 cut(s)	534, 555
SmlI	CTYRAG	1 cut(s)	1448
SmoI	CTYRAG	1 cut(s)	1448
SnaBI	TACGTA	1 cut(s)	469
SphI	GCATGC	3 cut(s)	191, 1093, 1542
SseBI	AGGCCT	1 cut(s)	203
SsiI	CCGC	5 cut(s)	764, 868, 1064, 1176, 1542
SspMI	CTAG	1 cut(s)	629
StuI	AGGCCT	1 cut(s)	203
StyD4I	CCNGG	2 cut(s)	159, 932
TaaI	ACNGT	3 cut(s)	607, 649, 1629
TaiI	ACGT	4 cut(s)	471, 882, 1040, 1085
TaqI	TCGA	3 cut(s)	941, 970, 1299
TatI	WGTACW	2 cut(s)	1164, 1404
TauI	GCSGC	1 cut(s)	767
TfiI	GAWTC	5 cut(s)	24, 381, 539, 1412, 1468
Tru1I	TTAA	6 cut(s)	551, 717, 894, 1052, 1079, 1350
Tru9I	TTAA	6 cut(s)	551, 717, 894, 1052, 1079, 1350
TscAI	CASTG	3 cut(s)	361, 652, 1566
TseFI	GTSAC	1 cut(s)	1231
TseI	GCWGC	5 cut(s)	557, 592, 811, 1610, 1613
Tsp45I	GTSAC	1 cut(s)	1231
TspDTI	ATGAA	7 cut(s)	296, 531, 861, 915, 1322, 1659, 1739
TspGWI	ACGGA	2 cut(s)	476, 795
TspRI	CASTG	3 cut(s)	361, 652, 1566
XagI	CCTNNNNNAGG	1 cut(s)	197
XceI	RCATGY	8 cut(s)	191, 214, 573, 676, 1093, 1165, 1368, 1542
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1529
XspI	CTAG	1 cut(s)	629
ZrmI	AGTACT	1 cut(s)	1406
Zsp2I	ATGCAT	1 cut(s)	822

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.