RchiOBHm_Chr6g0247361

peptidase inhibitor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015533
Physical Location & Seq
Forward (+)
3261506 .. 3262246
741 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 492 bp
ATGGAATTCACCAAGCTCGCTTTCCTAGGCATGTGCTCCATCACCTTGATCTCAATTCATATTTCTCCGGCCAATGCCGATCCATTGGCTGAAGAGTACGTCAAAGCACACAACAAATACCGTGCAGTGGAAAATGTTCCTCCACTTTCATGGGACGAAACAGTCGCAAATTATGCTAAAAAATATGCCAAGTCAAAAGCTGAGACTTGTGAAATGGTGCATTCAGATGGACCATATGGTGAATGCTTAGCCATGGGTTCTTACAACATGAAACCTGCAGAGGCGGTGAAGCTGTGGGCAGATGAGAAGAAATACTATGACCATGCCTCCAATACATGTGCTGAAGGCCAGGTATGCGGCCACTATACTCAGGTGGTTTGGGAAAAATCAACCCACGTTGGGTGTGCCAAGGTTCATTGCAAGAATGGAGGTACTTTTATCACATGCAACTATGATCCCCCCGGAAACTATATTGGCGAGAAACCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

163

Amino Acids

18.1

Weight (kDa)

6.15

Isoelectric Point (pI)

31.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 35 - 151 4.6e-23 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 283
AciI CCGC 2 cut(s) 284, 357
AclWI GGATC 2 cut(s) 74, 449
AcoI YGGCCR 2 cut(s) 69, 358
AcsI RAATTY 1 cut(s) 5
AcuI CTGAAG 2 cut(s) 111, 363
AfaI GTAC 2 cut(s) 98, 433
AfiI CCNNNNNNNGG 2 cut(s) 127, 399
AflIII ACRYGT 1 cut(s) 335
AjnI CCWGG 1 cut(s) 348
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AluBI AGCT 3 cut(s) 16, 200, 292
AluI AGCT 3 cut(s) 16, 200, 292
Alw21I GWGCWC 1 cut(s) 38
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 2 cut(s) 74, 449
AoxI GGCC 3 cut(s) 69, 346, 358
ApoI RAATTY 1 cut(s) 5
Asp700I GAANNNNTTC 2 cut(s) 135, 485
AspA2I CCTAGG 1 cut(s) 25
AspS9I GGNCC 1 cut(s) 230
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 3 cut(s) 34, 251, 298
AvaII GGWCC 1 cut(s) 230
AvrII CCTAGG 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 38
BccI CCATC 2 cut(s) 47, 221
BciT130I CCWGG 1 cut(s) 350
BcnI CCSGG 1 cut(s) 462
BcoDI GTCTC 1 cut(s) 197
BfaI CTAG 2 cut(s) 26, 490
BfmI CTRYAG 1 cut(s) 276
BfuAI ACCTGC 1 cut(s) 283
BisI GCNGC 1 cut(s) 358
BlnI CCTAGG 1 cut(s) 25
BlpI GCTNAGC 1 cut(s) 247
BlsI GCNGC 1 cut(s) 359
Bme1390I CCNGG 2 cut(s) 350, 462
Bme18I GGWCC 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 230
BmrFI CCNGG 2 cut(s) 350, 462
Bpu1102I GCTNAGC 1 cut(s) 247
BpuMI CCSGG 1 cut(s) 462
BsaJI CCNNGG 4 cut(s) 25, 252, 408, 460
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 399
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 350
BseDI CCNNGG 4 cut(s) 25, 252, 408, 460
BseLI CCNNNNNNNGG 2 cut(s) 127, 399
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 2 cut(s) 192, 383
BsgI GTGCAG 1 cut(s) 144
BshFI GGCC 3 cut(s) 71, 348, 360
BsiHKAI GWGCWC 1 cut(s) 38
BsiSI CCGG 2 cut(s) 68, 462
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 2 cut(s) 127, 399
BsmAI GTCTC 1 cut(s) 197
BsmFI GGGAC 1 cut(s) 167
BsmI GAATGC 2 cut(s) 220, 248
BsnI GGCC 3 cut(s) 71, 348, 360
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 3 cut(s) 48, 79, 454
Bsp1720I GCTNAGC 1 cut(s) 247
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 2 cut(s) 284, 357
BspANI GGCC 3 cut(s) 71, 348, 360
BspCNI CTCAG 2 cut(s) 193, 382
BspMAI CTGCAG 1 cut(s) 280
BspMI ACCTGC 1 cut(s) 283
BspPI GGATC 2 cut(s) 74, 449
BsrDI GCAATG 1 cut(s) 415
BssECI CCNNGG 4 cut(s) 25, 252, 408, 460
BssMI GATC 3 cut(s) 48, 79, 454
BssT1I CCWWGG 3 cut(s) 25, 252, 408
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 2 cut(s) 122, 163
Bst6I CTCTTC 1 cut(s) 87
BstAPI GCANNNNNTGC 1 cut(s) 173
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 3 cut(s) 201, 247, 369
BstDSI CCRYGG 1 cut(s) 252
BstKTI GATC 3 cut(s) 51, 82, 457
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 3 cut(s) 48, 79, 454
BstMWI GCNNNNNNNGC 2 cut(s) 173, 354
BstNI CCWGG 1 cut(s) 350
BstNSI RCATGY 3 cut(s) 34, 339, 447
BstSCI CCNGG 2 cut(s) 348, 460
BstSFI CTRYAG 1 cut(s) 276
BstXI CCANNNNNNTGG 1 cut(s) 150
BsuRI GGCC 3 cut(s) 71, 348, 360
BtgI CCRYGG 1 cut(s) 252
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 132
BveI ACCTGC 1 cut(s) 283
Cac8I GCNNGC 1 cut(s) 18
Cfr13I GGNCC 1 cut(s) 230
Csp6I GTAC 2 cut(s) 97, 432
CviAII CATG 7 cut(s) 31, 150, 253, 268, 323, 336, 444
CviJI RGCY 8 cut(s) 16, 71, 89, 200, 251, 292, 348, 360
CviKI_1 RGCY 8 cut(s) 16, 71, 89, 200, 251, 292, 348, 360
CviQI GTAC 2 cut(s) 97, 432
DdeI CTNAG 3 cut(s) 201, 247, 369
DpnI GATC 3 cut(s) 50, 81, 456
DpnII GATC 3 cut(s) 48, 79, 454
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 2 cut(s) 69, 358
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco130I CCWWGG 3 cut(s) 25, 252, 408
Eco47I GGWCC 1 cut(s) 230
Eco57I CTGAAG 2 cut(s) 111, 363
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 1 cut(s) 348
EcoT14I CCWWGG 3 cut(s) 25, 252, 408
ErhI CCWWGG 3 cut(s) 25, 252, 408
FaeI CATG 7 cut(s) 34, 153, 256, 271, 326, 339, 447
FaqI GGGAC 1 cut(s) 167
FatI CATG 7 cut(s) 30, 149, 252, 267, 322, 335, 443
FauNDI CATATG 1 cut(s) 235
Fnu4HI GCNGC 1 cut(s) 358
Fsp4HI GCNGC 1 cut(s) 358
FspBI CTAG 2 cut(s) 26, 490
GluI GCNGC 1 cut(s) 358
HaeIII GGCC 3 cut(s) 71, 348, 360
HapII CCGG 2 cut(s) 68, 462
Hin1II CATG 7 cut(s) 34, 153, 256, 271, 326, 339, 447
HpaII CCGG 2 cut(s) 68, 462
HphI GGTGA 3 cut(s) 34, 251, 298
Hpy188I TCNGA 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 338
HpyCH4III ACNGT 2 cut(s) 122, 163
HpyCH4IV ACGT 2 cut(s) 99, 396
HpyCH4V TGCA 5 cut(s) 125, 220, 278, 420, 447
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 354
HpyF3I CTNAG 3 cut(s) 201, 247, 369
HpySE526I ACGT 2 cut(s) 99, 396
Hsp92II CATG 7 cut(s) 34, 153, 256, 271, 326, 339, 447
Kzo9I GATC 3 cut(s) 48, 79, 454
LmnI GCTCC 1 cut(s) 41
LpnPI CCDG 6 cut(s) 81, 288, 335, 356, 362, 475
MaeI CTAG 2 cut(s) 26, 490
MaeII ACGT 2 cut(s) 99, 396
MalI GATC 3 cut(s) 50, 81, 456
MboI GATC 3 cut(s) 48, 79, 454
MboII GAAGA 2 cut(s) 104, 319
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 3 cut(s) 5, 54, 169
MnlI CCTC 4 cut(s) 150, 274, 337, 422
MroXI GAANNNNTTC 2 cut(s) 135, 485
MslI CAYNNNNRTG 2 cut(s) 148, 225
MspI CCGG 2 cut(s) 68, 462
MspR9I CCNGG 2 cut(s) 350, 462
Mva1269I GAATGC 2 cut(s) 220, 248
MvaI CCWGG 1 cut(s) 350
MwoI GCNNNNNNNGC 2 cut(s) 173, 354
NciI CCSGG 1 cut(s) 462
NcoI CCATGG 1 cut(s) 252
NdeI CATATG 1 cut(s) 235
NdeII GATC 3 cut(s) 48, 79, 454
NlaIII CATG 7 cut(s) 34, 153, 256, 271, 326, 339, 447
NspI RCATGY 3 cut(s) 34, 339, 447
PciI ACATGT 1 cut(s) 335
PctI GAATGC 2 cut(s) 220, 248
PdmI GAANNNNTTC 2 cut(s) 135, 485
PkrI GCNGC 1 cut(s) 359
PscI ACATGT 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 348
PspGI CCWGG 1 cut(s) 348
PspPI GGNCC 1 cut(s) 230
PstI CTGCAG 1 cut(s) 280
RsaI GTAC 2 cut(s) 98, 433
RsaNI GTAC 2 cut(s) 97, 432
RseI CAYNNNNRTG 2 cut(s) 148, 225
SatI GCNGC 1 cut(s) 358
Sau3AI GATC 3 cut(s) 48, 79, 454
Sau96I GGNCC 1 cut(s) 230
ScrFI CCNGG 2 cut(s) 350, 462
SduI GDGCHC 1 cut(s) 38
SfcI CTRYAG 1 cut(s) 276
SinI GGWCC 1 cut(s) 230
SmiMI CAYNNNNRTG 2 cut(s) 148, 225
Sse9I AATT 3 cut(s) 5, 54, 169
SsiI CCGC 2 cut(s) 284, 357
SspMI CTAG 2 cut(s) 26, 490
StyD4I CCNGG 2 cut(s) 348, 460
StyI CCWWGG 3 cut(s) 25, 252, 408
TaaI ACNGT 2 cut(s) 122, 163
TaiI ACGT 2 cut(s) 102, 399
TasI AATT 3 cut(s) 5, 54, 169
TauI GCSGC 1 cut(s) 360
TscAI CASTG 1 cut(s) 132
TspDTI ATGAA 4 cut(s) 47, 138, 284, 404
TspRI CASTG 1 cut(s) 132
VpaK11BI GGWCC 1 cut(s) 230
XapI RAATTY 1 cut(s) 5
XceI RCATGY 3 cut(s) 34, 339, 447
XmaJI CCTAGG 1 cut(s) 25
XmnI GAANNNNTTC 2 cut(s) 135, 485
XspI CTAG 2 cut(s) 26, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.