RchiOBHm_Chr6g0251551

Heparan-alpha-glucosaminide N-acetyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009646
Physical Location & Seq
Reverse (-)
6809947 .. 6815683
5737 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1266 bp
ATGGCCGACGATTATGTCCTCATTCCAAACGGAGAAGAAGACCATGCAGTAGTCATCGCCACCACTCCCAAGCCTCCACGTGTCGCCTCCCTCGACGTCTTCCGCGGCCTCTGCGTCTTCCTGATGATGGTGGTGGATTACGGCGGCTCGATTATTCCGGCGATTGCTCACTCGCCGTGGAACGGTCTCCACTTGGCTGACTTTGTGATGCCGTTCTTTCTCTTCATTGCCGGAGTTTCTCTTGCTCTGGTCTACAAGAGAGTGACTAACAGAGTTGAAGCTACATGGAAGGCCGTGTTTCGGGCCCTTAAACTTTTTCTCTTAGGTGTTCTTCTTCAAGGTGGTTATTTTCATGGAGTGAGATCCTTGACTTATGGAGTTGACATTGAAAGAATACGGTGGTTTGGCATTTTGCAGAGGATAGCCATTGGATATATTGTTGCTGCCTTATGCGAAATCTGGCTTTCACATCGCACCTCGAGGGAAGTGAGGTTTTTCAGGAGCTATTACTGGCACTGGTGTGTCATCTTTTTGTTATCAGCAATATACGCTGGGTTGCTGTATGGTTTGTATGTTCCAGATTGGGAATTCAAAGCATCAACACCTACCTCTTTGTCTCCTTCTGATGATACACATGTTTACGCGGTGAGATGTTCTGTGAGAGGAGATCTTGGACCTGGTTGTAATTCCGCTGGGATGATTGATCGTTACATTGTGGGTGTTGATCATCTCTATTCAAAACCCGTTTACAGGAACCTGAAGGATTGCAATATGTCTACTGATGGTCGGATTCCAGAGAGTTCGCCTTCATGGTGTCATACTCCTTTTGATCCTGAAGGTATATTAAGCACCCTAACAGCAGCAGTGACTTGCATAATTGGACTTCAATATGGTCACATTCTTGCACACATACAGGACCACAAAGGGCGCCTAAATATTTGGTCCTTGTTTTCAGTTTCAATGTTTGTCCTTGGATCATTTCTTGCCTTCATAGGCGTTCCTGTAAATAAATCTCTGTACACTATCAGTTATATGCTAATTACTTCTGCATCTGCAGGCATGACATTTTGCGCTCTATATCTGCTGATAGATGTTTACGGTTACCGGTGCATAACTTTTGTGTTGGAGTGGATGGGCATACATTCTTTGAGTATTTTCATAATTGTAACTTCCAACCTAGCTGTGATTGCGATTCAGGGATTCTACTGGACAGATCCTGAGAATAATATTGTTCACTGGATCATAGCACTTTTTGTGCACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
DUF1624

Protein Analysis

421

Amino Acids

47.3

Weight (kDa)

7.22

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGSNAT_cat PF07786 27 - 151 1.7e-06 Heparan-alpha-glucosaminide N-acetyltransferase, catalytic
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 14
AatII GACGTC 1 cut(s) 99
AbsI CCTCGAGG 1 cut(s) 478
AccB1I GGYRCC 1 cut(s) 927
AccI GTMKAC 2 cut(s) 252, 776
AccII CGCG 2 cut(s) 105, 644
AciI CCGC 5 cut(s) 103, 105, 144, 644, 690
AclWI GGATC 5 cut(s) 357, 824, 982, 1208, 1247
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 587
AcuI CTGAAG 2 cut(s) 779, 855
AcvI CACGTG 1 cut(s) 80
AcyI GRCGYC 2 cut(s) 96, 928
AfaI GTAC 1 cut(s) 1019
AfiI CCNNNNNNNGG 4 cut(s) 127, 182, 300, 750
AflIII ACRYGT 2 cut(s) 79, 634
AgeI ACCGGT 1 cut(s) 1104
AgsI TTSAA 7 cut(s) 278, 338, 389, 592, 738, 887, 960
AjnI CCWGG 1 cut(s) 676
AleI CACNNNNGTG 1 cut(s) 519
AluBI AGCT 3 cut(s) 281, 504, 1181
AluI AGCT 3 cut(s) 281, 504, 1181
Alw21I GWGCWC 1 cut(s) 1260
Alw26I GTCTC 2 cut(s) 191, 621
Alw44I GTGCAC 1 cut(s) 1256
AlwI GGATC 5 cut(s) 357, 824, 982, 1208, 1247
AlwNI CAGNNNCTG 1 cut(s) 1217
Ama87I CYCGRG 1 cut(s) 478
AoxI GGCC 4 cut(s) 3, 106, 291, 303
ApaI GGGCCC 1 cut(s) 307
ApaLI GTGCAC 1 cut(s) 1256
ApeKI GCWGC 2 cut(s) 443, 860
ApoI RAATTY 1 cut(s) 587
AsiGI ACCGGT 1 cut(s) 1104
AspLEI GCGC 2 cut(s) 930, 1073
AspS9I GGNCC 5 cut(s) 303, 304, 674, 916, 942
AsuHPI GGTGA 1 cut(s) 658
AvaI CYCGRG 1 cut(s) 478
AvaII GGWCC 3 cut(s) 674, 916, 942
BaeGI GKGCMC 2 cut(s) 307, 1260
BaeI ACNNNNGTAYC 2 cut(s) 621, 654
BanI GGYRCC 1 cut(s) 927
BanII GRGCYC 1 cut(s) 307
BbrPI CACGTG 1 cut(s) 80
BbsI GAAGAC 3 cut(s) 45, 91, 109
Bbv12I GWGCWC 1 cut(s) 1260
BbvI GCAGC 2 cut(s) 430, 872
BccI CCATC 3 cut(s) 121, 776, 1126
BceAI ACGGC 4 cut(s) 157, 160, 196, 278
BciT130I CCWGG 1 cut(s) 678
BclI TGATCA 1 cut(s) 724
BcoDI GTCTC 2 cut(s) 191, 621
BfaI CTAG 1 cut(s) 1178
BfmI CTRYAG 1 cut(s) 1053
BfoI RGCGCY 1 cut(s) 931
BglII AGATCT 1 cut(s) 667
BisI GCNGC 4 cut(s) 106, 145, 444, 861
BlsI GCNGC 4 cut(s) 107, 146, 445, 862
Bme1390I CCNGG 1 cut(s) 678
Bme18I GGWCC 3 cut(s) 674, 916, 942
BmeT110I CYCGRG 1 cut(s) 478
BmgT120I GGNCC 5 cut(s) 303, 304, 674, 916, 942
BmiI GGNNCC 3 cut(s) 305, 755, 929
BmrFI CCNGG 1 cut(s) 678
BmsI GCATC 3 cut(s) 198, 605, 1058
BpiI GAAGAC 3 cut(s) 45, 91, 109
BsaAI YACGTR 1 cut(s) 80
BsaHI GRCGYC 2 cut(s) 96, 928
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 3 cut(s) 103, 176, 970
BsaWI WCCGGW 1 cut(s) 1104
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 182, 300, 750
Bse118I RCCGGY 1 cut(s) 1104
Bse1I ACTGG 4 cut(s) 515, 521, 1211, 1241
Bse3DI GCAATG 1 cut(s) 225
BseBI CCWGG 1 cut(s) 678
BseDI CCNNGG 3 cut(s) 103, 176, 970
BseGI GGATG 2 cut(s) 702, 1137
BseLI CCNNNNNNNGG 4 cut(s) 127, 182, 300, 750
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 1209
BseNI ACTGG 4 cut(s) 515, 521, 1211, 1241
BseRI GAGGAG 1 cut(s) 678
BseSI GKGCMC 2 cut(s) 307, 1260
BseXI GCAGC 2 cut(s) 430, 872
BseYI CCCAGC 2 cut(s) 551, 692
Bsh1236I CGCG 2 cut(s) 105, 644
BshFI GGCC 4 cut(s) 5, 108, 293, 305
BshNI GGYRCC 1 cut(s) 927
BshTI ACCGGT 1 cut(s) 1104
BsiHKAI GWGCWC 1 cut(s) 1260
BsiHKCI CYCGRG 1 cut(s) 478
BsiSI CCGG 3 cut(s) 158, 231, 1105
BslI CCNNNNNNNGG 4 cut(s) 127, 182, 300, 750
BsmAI GTCTC 2 cut(s) 191, 621
BsnI GGCC 4 cut(s) 5, 108, 293, 305
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 478
Bsp120I GGGCCC 1 cut(s) 303
Bsp1286I GDGCHC 2 cut(s) 307, 1260
Bsp1407I TGTACA 1 cut(s) 1017
Bsp143I GATC 8 cut(s) 362, 667, 703, 724, 829, 974, 1213, 1239
BspACI CCGC 5 cut(s) 103, 105, 144, 644, 690
BspANI GGCC 4 cut(s) 5, 108, 293, 305
BspCNI CTCAG 1 cut(s) 1210
BspFNI CGCG 2 cut(s) 105, 644
BspLI GGNNCC 3 cut(s) 305, 755, 929
BspMAI CTGCAG 1 cut(s) 1057
BspPI GGATC 5 cut(s) 357, 824, 982, 1208, 1247
BspT107I GGYRCC 1 cut(s) 927
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 225
BsrFI RCCGGY 1 cut(s) 1104
BsrGI TGTACA 1 cut(s) 1017
BsrI ACTGG 4 cut(s) 515, 521, 1211, 1241
BssAI RCCGGY 1 cut(s) 1104
BssECI CCNNGG 3 cut(s) 103, 176, 970
BssMI GATC 8 cut(s) 362, 667, 703, 724, 829, 974, 1213, 1239
BssNI GRCGYC 2 cut(s) 96, 928
BssT1I CCWWGG 1 cut(s) 970
Bst2UI CCWGG 1 cut(s) 678
Bst4CI ACNGT 3 cut(s) 185, 399, 1100
Bst6I CTCTTC 1 cut(s) 227
BstACI GRCGYC 2 cut(s) 96, 928
BstAUI TGTACA 1 cut(s) 1017
BstBAI YACGTR 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 1057
BstDEI CTNAG 2 cut(s) 322, 1218
BstDSI CCRYGG 2 cut(s) 103, 176
BstEII GGTNACC 1 cut(s) 1100
BstF5I GGATG 2 cut(s) 702, 1137
BstFNI CGCG 2 cut(s) 105, 644
BstH2I RGCGCY 1 cut(s) 931
BstHHI GCGC 2 cut(s) 930, 1073
BstKTI GATC 8 cut(s) 365, 670, 706, 727, 832, 977, 1216, 1242
BstMAI GTCTC 2 cut(s) 191, 621
BstMBI GATC 8 cut(s) 362, 667, 703, 724, 829, 974, 1213, 1239
BstMWI GCNNNNNNNGC 3 cut(s) 111, 548, 1187
BstNI CCWGG 1 cut(s) 678
BstNSI RCATGY 1 cut(s) 638
BstPI GGTNACC 1 cut(s) 1100
BstSCI CCNGG 1 cut(s) 676
BstSFI CTRYAG 1 cut(s) 1053
BstSLI GKGCMC 2 cut(s) 307, 1260
BstUI CGCG 2 cut(s) 105, 644
BstV1I GCAGC 2 cut(s) 430, 872
BstV2I GAAGAC 3 cut(s) 45, 91, 109
BstX2I RGATCY 3 cut(s) 362, 667, 1213
BstYI RGATCY 3 cut(s) 362, 667, 1213
BsuRI GGCC 4 cut(s) 5, 108, 293, 305
BtgI CCRYGG 2 cut(s) 103, 176
BtgZI GCGATG 2 cut(s) 40, 455
BtsCI GGATG 2 cut(s) 702, 1137
BtsI GCAGTG 1 cut(s) 870
BtsIMutI CAGTG 3 cut(s) 514, 870, 1234
Cac8I GCNNGC 1 cut(s) 1057
CaiI CAGNNNCTG 1 cut(s) 1217
CfoI GCGC 2 cut(s) 930, 1073
Cfr10I RCCGGY 1 cut(s) 1104
Cfr13I GGNCC 5 cut(s) 303, 304, 674, 916, 942
Cfr42I CCGCGG 1 cut(s) 106
CseI GACGC 1 cut(s) 103
CsiI ACCWGGT 1 cut(s) 676
Csp6I GTAC 1 cut(s) 1018
CspAI ACCGGT 1 cut(s) 1104
CviAII CATG 6 cut(s) 44, 285, 353, 635, 810, 1060
CviQI GTAC 1 cut(s) 1018
DdeI CTNAG 2 cut(s) 322, 1218
DinI GGCGCC 1 cut(s) 929
DpnI GATC 8 cut(s) 364, 669, 705, 726, 831, 976, 1215, 1241
DpnII GATC 8 cut(s) 362, 667, 703, 724, 829, 974, 1213, 1239
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 970
Eco24I GRGCYC 1 cut(s) 307
Eco31I GGTCTC 1 cut(s) 191
Eco47I GGWCC 3 cut(s) 674, 916, 942
Eco57I CTGAAG 2 cut(s) 779, 855
Eco72I CACGTG 1 cut(s) 80
Eco88I CYCGRG 1 cut(s) 478
Eco91I GGTNACC 1 cut(s) 1100
EcoO109I RGGNCCY 1 cut(s) 304
EcoO65I GGTNACC 1 cut(s) 1100
EcoRI GAATTC 1 cut(s) 587
EcoRII CCWGG 1 cut(s) 676
EcoT14I CCWWGG 1 cut(s) 970
EcoT38I GRGCYC 1 cut(s) 307
EgeI GGCGCC 1 cut(s) 929
EheI GGCGCC 1 cut(s) 929
ErhI CCWWGG 1 cut(s) 970
FaeI CATG 6 cut(s) 47, 288, 356, 638, 813, 1063
FatI CATG 6 cut(s) 43, 284, 352, 634, 809, 1059
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 2 cut(s) 252, 776
Fnu4HI GCNGC 4 cut(s) 106, 145, 444, 861
FokI GGATG 2 cut(s) 709, 1144
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 4 cut(s) 106, 145, 444, 861
FspBI CTAG 1 cut(s) 1178
GlaI GCGC 2 cut(s) 929, 1072
GluI GCNGC 4 cut(s) 106, 145, 444, 861
GsaI CCCAGC 2 cut(s) 555, 696
HaeII RGCGCY 1 cut(s) 931
HaeIII GGCC 4 cut(s) 5, 108, 293, 305
HapII CCGG 3 cut(s) 158, 231, 1105
HgaI GACGC 1 cut(s) 103
HhaI GCGC 2 cut(s) 930, 1073
Hin1I GRCGYC 2 cut(s) 96, 928
Hin1II CATG 6 cut(s) 47, 288, 356, 638, 813, 1063
Hin6I GCGC 2 cut(s) 928, 1071
HinP1I GCGC 2 cut(s) 928, 1071
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 3 cut(s) 790, 1192, 1200
HpaII CCGG 3 cut(s) 158, 231, 1105
HphI GGTGA 1 cut(s) 658
Hpy166II GTNNAC 9 cut(s) 253, 382, 640, 748, 777, 1020, 1096, 1234, 1258
Hpy188I TCNGA 2 cut(s) 625, 789
Hpy188III TCNNGA 6 cut(s) 121, 499, 578, 794, 833, 1217
Hpy8I GTNNAC 9 cut(s) 253, 382, 640, 748, 777, 1020, 1096, 1234, 1258
Hpy99I CGWCG 2 cut(s) 11, 98
HpyAV CCTTC 6 cut(s) 283, 630, 754, 816, 830, 997
HpyCH4III ACNGT 3 cut(s) 185, 399, 1100
HpyCH4IV ACGT 2 cut(s) 79, 96
HpyCH4V TGCA 9 cut(s) 47, 415, 768, 873, 905, 1049, 1055, 1110, 1258
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 548, 1187
HpyF3I CTNAG 2 cut(s) 322, 1218
HpySE526I ACGT 2 cut(s) 79, 96
Hsp92I GRCGYC 2 cut(s) 96, 928
Hsp92II CATG 6 cut(s) 47, 288, 356, 638, 813, 1063
HspAI GCGC 2 cut(s) 928, 1071
KasI GGCGCC 1 cut(s) 927
Ksp22I TGATCA 1 cut(s) 724
KspI CCGCGG 1 cut(s) 106
Kzo9I GATC 8 cut(s) 362, 667, 703, 724, 829, 974, 1213, 1239
LmnI GCTCC 1 cut(s) 501
Lsp1109I GCAGC 2 cut(s) 430, 872
LweI GCATC 3 cut(s) 198, 605, 1058
MabI ACCWGGT 1 cut(s) 676
MaeI CTAG 1 cut(s) 1178
MaeII ACGT 2 cut(s) 79, 96
MaeIII GTNAC 6 cut(s) 262, 707, 865, 893, 1100, 1165
MalI GATC 8 cut(s) 364, 669, 705, 726, 831, 976, 1215, 1241
MboI GATC 8 cut(s) 362, 667, 703, 724, 829, 974, 1213, 1239
MboII GAAGA 7 cut(s) 47, 50, 91, 109, 214, 323, 326
MflI RGATCY 3 cut(s) 362, 667, 1213
MhlI GDGCHC 2 cut(s) 307, 1260
MluCI AATT 5 cut(s) 587, 685, 876, 1038, 1161
Mly113I GGCGCC 1 cut(s) 928
MmeI TCCRAC 3 cut(s) 767, 1104, 1197
MseI TTAA 2 cut(s) 309, 845
MslI CAYNNNNRTG 1 cut(s) 519
MspA1I CMGCKG 2 cut(s) 105, 692
MspI CCGG 3 cut(s) 158, 231, 1105
MspR9I CCNGG 1 cut(s) 678
MvaI CCWGG 1 cut(s) 678
MvnI CGCG 2 cut(s) 105, 644
MwoI GCNNNNNNNGC 3 cut(s) 111, 548, 1187
NarI GGCGCC 1 cut(s) 928
NdeII GATC 8 cut(s) 362, 667, 703, 724, 829, 974, 1213, 1239
NlaIII CATG 6 cut(s) 47, 288, 356, 638, 813, 1063
NlaIV GGNNCC 3 cut(s) 305, 755, 929
NmuCI GTSAC 3 cut(s) 262, 865, 893
NspI RCATGY 1 cut(s) 638
OliI CACNNNNGTG 1 cut(s) 519
PaeR7I CTCGAG 1 cut(s) 478
PciI ACATGT 1 cut(s) 634
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PfeI GAWTC 3 cut(s) 790, 1192, 1200
PinAI ACCGGT 1 cut(s) 1104
PkrI GCNGC 4 cut(s) 107, 146, 445, 862
PluTI GGCGCC 1 cut(s) 931
PmaCI CACGTG 1 cut(s) 80
PmlI CACGTG 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 80
PscI ACATGT 1 cut(s) 634
Psp6I CCWGG 1 cut(s) 676
PspCI CACGTG 1 cut(s) 80
PspEI GGTNACC 1 cut(s) 1100
PspFI CCCAGC 2 cut(s) 551, 692
PspGI CCWGG 1 cut(s) 676
PspN4I GGNNCC 3 cut(s) 305, 755, 929
PspOMI GGGCCC 1 cut(s) 303
PspPI GGNCC 5 cut(s) 303, 304, 674, 916, 942
PspXI VCTCGAGB 1 cut(s) 478
PstI CTGCAG 1 cut(s) 1057
PstNI CAGNNNCTG 1 cut(s) 1217
PsuI RGATCY 3 cut(s) 362, 667, 1213
RsaI GTAC 1 cut(s) 1019
RsaNI GTAC 1 cut(s) 1018
RseI CAYNNNNRTG 1 cut(s) 519
SacII CCGCGG 1 cut(s) 106
SaqAI TTAA 2 cut(s) 309, 845
SatI GCNGC 4 cut(s) 106, 145, 444, 861
Sau3AI GATC 8 cut(s) 362, 667, 703, 724, 829, 974, 1213, 1239
Sau96I GGNCC 5 cut(s) 303, 304, 674, 916, 942
ScrFI CCNGG 1 cut(s) 678
SduI GDGCHC 2 cut(s) 307, 1260
SexAI ACCWGGT 1 cut(s) 676
SfaNI GCATC 3 cut(s) 198, 605, 1058
SfcI CTRYAG 1 cut(s) 1053
SfoI GGCGCC 1 cut(s) 929
Sfr274I CTCGAG 1 cut(s) 478
Sfr303I CCGCGG 1 cut(s) 106
SgrBI CCGCGG 1 cut(s) 106
SinI GGWCC 3 cut(s) 674, 916, 942
SlaI CTCGAG 1 cut(s) 478
SmiMI CAYNNNNRTG 1 cut(s) 519
SmlI CTYRAG 1 cut(s) 478
SmoI CTYRAG 1 cut(s) 478
Sse9I AATT 5 cut(s) 587, 685, 876, 1038, 1161
SsiI CCGC 5 cut(s) 103, 105, 144, 644, 690
SspDI GGCGCC 1 cut(s) 927
SspI AATATT 2 cut(s) 937, 1228
SspMI CTAG 1 cut(s) 1178
StyD4I CCNGG 1 cut(s) 676
StyI CCWWGG 1 cut(s) 970
TaaI ACNGT 3 cut(s) 185, 399, 1100
TaiI ACGT 2 cut(s) 82, 99
TaqI TCGA 3 cut(s) 93, 149, 479
TasI AATT 5 cut(s) 587, 685, 876, 1038, 1161
TatI WGTACW 1 cut(s) 1017
TauI GCSGC 2 cut(s) 108, 147
TfiI GAWTC 3 cut(s) 790, 1192, 1200
Tru1I TTAA 2 cut(s) 309, 845
Tru9I TTAA 2 cut(s) 309, 845
TscAI CASTG 3 cut(s) 521, 870, 1241
TseFI GTSAC 3 cut(s) 262, 865, 893
TseI GCWGC 2 cut(s) 443, 860
Tsp45I GTSAC 3 cut(s) 262, 865, 893
TspDTI ATGAA 5 cut(s) 214, 341, 798, 979, 1147
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 3 cut(s) 521, 870, 1241
VneI GTGCAC 1 cut(s) 1256
VpaK11BI GGWCC 3 cut(s) 674, 916, 942
XapI RAATTY 1 cut(s) 587
XceI RCATGY 1 cut(s) 638
XhoI CTCGAG 1 cut(s) 478
XmiI GTMKAC 2 cut(s) 252, 776
XspI CTAG 1 cut(s) 1178
ZraI GACGTC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.