RchiOBHm_Chr6g0252251

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0014916
Physical Location & Seq
Forward (+)
7430459 .. 7432935
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1539 bp
ATGTTGCCAAGGAGCCGAACTCTTCCGAGCAGAATCCACCATGGAGCTGTGGAAGAGAGGCATGATATCAGGCACTATTTGCAAGTAGAAGTTCAACCTAAAGTCACTGAATCTGAAGCTATAAACCCTCAGTCCAACTATTCCAAATGCTTTGATGATGATGGTCGCTTGAAGCGAACAGGAACTTTTTGGACTGCAACGTCACATATTATCACTGCAGTTATAGGATCTGGAGTTCTTTCTTTAGCATGGGCAATTGCACAACTTGGTTGGGTTGCAGGACCAATTGTCCTCGCTCTTTTTGCCATTGTCAATCTGTACACTTCCAATCTCCTATCCATGTGCTATCGGGCCGGGGACCCTGTCACGGGACAGAGAAATTATACCTATATGGATGCTGTCAAGGCCAACTTAGGAGGAAGAAAAGTTATGTTATGTGGTTTAGTCCAGTACTTGAATTTGTTTGGGGTAGCAATTGGATATACCATTGCATCTTCTGTCAGCATGATGGCAATAAAGAGATCAAACTGCTACCACAAAAGTGGAGGGAAAGATCCATGCCATATGTCTAGCAATGGTTATATGATAACATTTGGAATCATAGAGGTGATATTTTCACAAATCAAAGATTTCAATGAAGTATGGTGGCTCTCAATAGTAGCAGCCATTATGTCATTCACATACTCCACTGTCGGCCTTGGACTTGGCATTGGTAAAGTTGCAGGAAATGGGGATTTTAAAGGAAGCCTGTTGGGTATTAGCATAGGGACAGTTACTCATTCTGGAGCAGTAGTCACTTCTACAGAAAAGATGTGGAGGACTATGCAAGCTCTTGGAGCTATTGCATTTGCTTACTCTTATTCTTTGGTCCTCATAGAAATTCAGGATACTATAAGATCTCCTCCTGCAGAACATAAGACCATGAAGAAGGCAACTATATTCAGCATCACTGTCACAACAGTGTTCTATATGCTCTGTGGATGCTTTGGGTATGCAGCGTTTGGCGATCTTGCCCCAGGAAACCTCTTAACTGGCTTTGGATTCTACAACCCCTACTGGCTTCTAGACATTGCAAATGTTGCAATTGTTGTCCACCTAGTAGGTGCATTTCAGGTCTTTTGCCAGCCATTATTTGCATTTGTGGAGAAGTGGAGTGCACAAAAGTGGCCGAAAAGCGACTTTGTGACAGCCGAGTATGATATACCTATCCCTTTCTATGGTGTGTACCAACTCAACCTGTTTCGCTTGGTATGGAGGACCATGTTTGTTATATTGACTACCCTTATATCCTTGCTCCTACCATTCTTCAATGATATTGTTGGGATACTTGGAGCCTTTGGATTCTGGCCATTGACTGTTTATTTTCCGGTTGAGATGTACATTGCTCAACAGAAGATTGAGAGATGGAGTAGTAGATGGCTTGGACTTCAGATACTAAGTGTGAGTTGTCTCCTTGTCTCCATAGTTGCTGCTGTTGGCTCTGTCGCCGGCGTAGTTCTAGATCTGAAGACATATAAACCCTTCAAAACTACTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

512

Amino Acids

56.67

Weight (kDa)

8.95

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 59 - 499 7.7e-116 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09220 AT5G63850
fragaria_vesca FvH4_2g01330
malus_domestica MD05G1120400.v1.1
prunus_persica Prupe.4G277700_v2.0.a1 Prupe.8G164200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0252251
rosa_laevigata RLG00000015401
rosa_multiflora Rmu_sc0006252.1_g000018 Rmu_sc0009799.1_g000021
rosa_roxburghii Rroxscaffold_7G00216210
rosa_rugosa Rorug05G0506400
rosa_samantha Rh6AG020200 Rh6BG015800 Rh6CG013000
rosa_wichuraiana Rw6G001660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 199
AclWI GGATC 2 cut(s) 235, 548
AcoI YGGCCR 2 cut(s) 1166, 1346
AcsI RAATTY 2 cut(s) 457, 879
AcuI CTGAAG 3 cut(s) 135, 1412, 1526
AfaI GTAC 4 cut(s) 320, 452, 1226, 1379
AfiI CCNNNNNNNGG 3 cut(s) 367, 368, 1217
AgsI TTSAA 6 cut(s) 95, 172, 457, 634, 1309, 1525
AhdI GACNNNNNGTC 1 cut(s) 287
AjnI CCWGG 1 cut(s) 1015
AleI CACNNNNGTG 3 cut(s) 540, 959, 1162
AloI GAACNNNNNNTCC 2 cut(s) 219, 251
AluBI AGCT 4 cut(s) 47, 119, 830, 839
AluI AGCT 4 cut(s) 47, 119, 830, 839
Alw21I GWGCWC 1 cut(s) 1159
Alw26I GTCTC 2 cut(s) 1454, 1462
Alw44I GTGCAC 1 cut(s) 1155
AlwI GGATC 2 cut(s) 235, 548
AoxI GGCC 5 cut(s) 351, 405, 694, 1166, 1346
ApaLI GTGCAC 1 cut(s) 1155
ApeKI GCWGC 3 cut(s) 662, 995, 1469
ApoI RAATTY 2 cut(s) 457, 879
AspS9I GGNCC 5 cut(s) 281, 351, 358, 868, 1257
AsuC2I CCSGG 1 cut(s) 355
AsuHPI GGTGA 1 cut(s) 619
AvaII GGWCC 4 cut(s) 281, 358, 868, 1257
BaeGI GKGCMC 1 cut(s) 1159
BalI TGGCCA 1 cut(s) 1348
BbsI GAAGAC 1 cut(s) 1514
Bbv12I GWGCWC 1 cut(s) 1159
BbvI GCAGC 3 cut(s) 674, 1007, 1456
BccI CCATC 4 cut(s) 155, 502, 1398, 1410
BciT130I CCWGG 1 cut(s) 1017
BciVI GTATCC 2 cut(s) 880, 1317
BcnI CCSGG 1 cut(s) 355
BcoDI GTCTC 2 cut(s) 1454, 1462
BfaI CTAG 4 cut(s) 570, 1064, 1097, 1499
BfmI CTRYAG 3 cut(s) 216, 801, 906
BfuI GTATCC 2 cut(s) 880, 1317
BglII AGATCT 2 cut(s) 896, 1501
BisI GCNGC 3 cut(s) 663, 996, 1470
BlsI GCNGC 3 cut(s) 664, 997, 1471
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 2 cut(s) 355, 1017
Bme18I GGWCC 4 cut(s) 281, 358, 868, 1257
BmeRI GACNNNNNGTC 1 cut(s) 287
BmgT120I GGNCC 5 cut(s) 281, 351, 358, 868, 1257
BmiI GGNNCC 4 cut(s) 14, 359, 360, 1333
BmrFI CCNGG 2 cut(s) 355, 1017
BmsI GCATC 4 cut(s) 385, 500, 954, 971
BpiI GAAGAC 1 cut(s) 1514
BplI GAGNNNNNCTC 1 cut(s) 36
BpmI CTGGAG 2 cut(s) 252, 804
BpuMI CCSGG 1 cut(s) 355
BsaJI CCNNGG 5 cut(s) 8, 40, 354, 697, 1015
BsaWI WCCGGW 1 cut(s) 1366
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 3 cut(s) 367, 368, 1217
Bse118I RCCGGY 1 cut(s) 1487
Bse1I ACTGG 3 cut(s) 448, 1036, 1061
Bse3DI GCAATG 4 cut(s) 486, 580, 1068, 1380
BseBI CCWGG 1 cut(s) 1017
BseDI CCNNGG 5 cut(s) 8, 40, 354, 697, 1015
BseGI GGATG 2 cut(s) 400, 986
BseLI CCNNNNNNNGG 3 cut(s) 367, 368, 1217
BseMI GCAATG 4 cut(s) 486, 580, 1068, 1380
BseMII CTCAG 1 cut(s) 143
BseNI ACTGG 3 cut(s) 448, 1036, 1061
BseRI GAGGAG 1 cut(s) 891
BseSI GKGCMC 1 cut(s) 1159
BseXI GCAGC 3 cut(s) 674, 1007, 1456
BshFI GGCC 5 cut(s) 353, 407, 696, 1168, 1348
BsiHKAI GWGCWC 1 cut(s) 1159
BsiSI CCGG 3 cut(s) 354, 1367, 1488
BslFI GGGAC 3 cut(s) 371, 384, 781
BslI CCNNNNNNNGG 3 cut(s) 367, 368, 1217
BsmAI GTCTC 2 cut(s) 1454, 1462
BsmFI GGGAC 3 cut(s) 371, 384, 781
BsnI GGCC 5 cut(s) 353, 407, 696, 1168, 1348
Bsp1286I GDGCHC 1 cut(s) 1159
Bsp1407I TGTACA 2 cut(s) 318, 1377
Bsp143I GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
Bsp19I CCATGG 1 cut(s) 40
BspANI GGCC 5 cut(s) 353, 407, 696, 1168, 1348
BspCNI CTCAG 1 cut(s) 142
BspLI GGNNCC 4 cut(s) 14, 359, 360, 1333
BspMAI CTGCAG 2 cut(s) 220, 910
BspPI GGATC 2 cut(s) 235, 548
BsrDI GCAATG 4 cut(s) 486, 580, 1068, 1380
BsrFI RCCGGY 1 cut(s) 1487
BsrGI TGTACA 2 cut(s) 318, 1377
BsrI ACTGG 3 cut(s) 448, 1036, 1061
BssAI RCCGGY 1 cut(s) 1487
BssECI CCNNGG 5 cut(s) 8, 40, 354, 697, 1015
BssMI GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
BssT1I CCWWGG 3 cut(s) 8, 40, 697
Bst2UI CCWGG 1 cut(s) 1017
Bst4CI ACNGT 5 cut(s) 691, 772, 952, 961, 1357
Bst6I CTCTTC 2 cut(s) 27, 48
BstAPI GCANNNNNTGC 2 cut(s) 79, 1079
BstAUI TGTACA 2 cut(s) 318, 1377
BstC8I GCNNGC 3 cut(s) 828, 1124, 1489
BstDEI CTNAG 3 cut(s) 129, 412, 1436
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 2 cut(s) 400, 986
BstKTI GATC 6 cut(s) 230, 524, 556, 899, 1009, 1504
BstMAI GTCTC 2 cut(s) 1454, 1462
BstMBI GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
BstMWI GCNNNNNNNGC 5 cut(s) 79, 302, 404, 836, 1079
BstNI CCWGG 1 cut(s) 1017
BstSCI CCNGG 2 cut(s) 353, 1015
BstSFI CTRYAG 3 cut(s) 216, 801, 906
BstSLI GKGCMC 1 cut(s) 1159
BstV1I GCAGC 3 cut(s) 674, 1007, 1456
BstV2I GAAGAC 1 cut(s) 1514
BstX2I RGATCY 4 cut(s) 227, 553, 896, 1501
BstXI CCANNNNNNTGG 1 cut(s) 542
BstYI RGATCY 4 cut(s) 227, 553, 896, 1501
BsuI GTATCC 2 cut(s) 880, 1317
BsuRI GGCC 5 cut(s) 353, 407, 696, 1168, 1348
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 2 cut(s) 400, 986
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 5 cut(s) 105, 213, 687, 948, 966
Cac8I GCNNGC 3 cut(s) 828, 1124, 1489
Cfr10I RCCGGY 1 cut(s) 1487
Cfr13I GGNCC 5 cut(s) 281, 351, 358, 868, 1257
Csp6I GTAC 4 cut(s) 319, 451, 1225, 1378
CviAII CATG 8 cut(s) 41, 62, 249, 340, 505, 558, 922, 1261
CviQI GTAC 4 cut(s) 319, 451, 1225, 1378
DdeI CTNAG 3 cut(s) 129, 412, 1436
DpnI GATC 6 cut(s) 229, 523, 555, 898, 1008, 1503
DpnII GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
DraI TTTAAA 1 cut(s) 739
DrdI GACNNNNNNGTC 1 cut(s) 199
DriI GACNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 199
EaeI YGGCCR 2 cut(s) 1166, 1346
Eam1104I CTCTTC 2 cut(s) 27, 48
Eam1105I GACNNNNNGTC 1 cut(s) 287
EarI CTCTTC 2 cut(s) 27, 48
Eco130I CCWWGG 3 cut(s) 8, 40, 697
Eco32I GATATC 1 cut(s) 67
Eco47I GGWCC 4 cut(s) 281, 358, 868, 1257
Eco57I CTGAAG 3 cut(s) 135, 1412, 1526
EcoO109I RGGNCCY 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 1015
EcoRV GATATC 1 cut(s) 67
EcoT14I CCWWGG 3 cut(s) 8, 40, 697
ErhI CCWWGG 3 cut(s) 8, 40, 697
FaeI CATG 8 cut(s) 44, 65, 252, 343, 508, 561, 925, 1264
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 3 cut(s) 371, 384, 781
FatI CATG 8 cut(s) 40, 61, 248, 339, 504, 557, 921, 1260
FauNDI CATATG 1 cut(s) 564
Fnu4HI GCNGC 3 cut(s) 663, 996, 1470
FokI GGATG 2 cut(s) 407, 993
Fsp4HI GCNGC 3 cut(s) 663, 996, 1470
FspBI CTAG 4 cut(s) 570, 1064, 1097, 1499
GluI GCNGC 3 cut(s) 663, 996, 1470
GsuI CTGGAG 2 cut(s) 252, 804
HaeIII GGCC 5 cut(s) 353, 407, 696, 1168, 1348
HapII CCGG 3 cut(s) 354, 1367, 1488
Hin1II CATG 8 cut(s) 44, 65, 252, 343, 508, 561, 925, 1264
HinfI GANTC 5 cut(s) 33, 110, 597, 1041, 1341
HpaII CCGG 3 cut(s) 354, 1367, 1488
HphI GGTGA 1 cut(s) 619
Hpy166II GTNNAC 4 cut(s) 321, 1093, 1157, 1225
Hpy188I TCNGA 4 cut(s) 27, 115, 1431, 1506
Hpy188III TCNNGA 5 cut(s) 231, 783, 884, 1064, 1499
Hpy8I GTNNAC 4 cut(s) 321, 1093, 1157, 1225
HpyAV CCTTC 2 cut(s) 922, 1531
HpyCH4III ACNGT 5 cut(s) 691, 772, 952, 961, 1357
HpyCH4IV ACGT 1 cut(s) 200
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 302, 404, 836, 1079
HpyF3I CTNAG 3 cut(s) 129, 412, 1436
HpySE526I ACGT 1 cut(s) 200
Hsp92II CATG 8 cut(s) 44, 65, 252, 343, 508, 561, 925, 1264
KflI GGGWCCC 1 cut(s) 358
KroI GCCGGC 1 cut(s) 1487
KroNI GCCGGC 1 cut(s) 1489
Kzo9I GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
LmnI GCTCC 6 cut(s) 12, 44, 785, 836, 1299, 1331
Lsp1109I GCAGC 3 cut(s) 674, 1007, 1456
LweI GCATC 4 cut(s) 385, 500, 954, 971
MaeI CTAG 4 cut(s) 570, 1064, 1097, 1499
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 7 cut(s) 103, 201, 364, 772, 793, 952, 1183
MalI GATC 6 cut(s) 229, 523, 555, 898, 1008, 1503
MboI GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
MboII GAAGA 8 cut(s) 14, 65, 432, 486, 937, 1297, 1405, 1519
MfeI CAATTG 4 cut(s) 255, 285, 474, 1083
MflI RGATCY 4 cut(s) 227, 553, 896, 1501
MhlI GDGCHC 1 cut(s) 1159
MlsI TGGCCA 1 cut(s) 1348
MluCI AATT 7 cut(s) 255, 285, 379, 457, 474, 879, 1083
MluNI TGGCCA 1 cut(s) 1348
MmeI TCCRAC 1 cut(s) 159
Mox20I TGGCCA 1 cut(s) 1348
MreI CGCCGGCG 1 cut(s) 1487
MroNI GCCGGC 1 cut(s) 1487
MscI TGGCCA 1 cut(s) 1348
MseI TTAA 2 cut(s) 738, 1028
MslI CAYNNNNRTG 4 cut(s) 540, 605, 959, 1162
Msp20I TGGCCA 1 cut(s) 1348
MspI CCGG 3 cut(s) 354, 1367, 1488
MspR9I CCNGG 2 cut(s) 355, 1017
MunI CAATTG 4 cut(s) 255, 285, 474, 1083
MvaI CCWGG 1 cut(s) 1017
MwoI GCNNNNNNNGC 5 cut(s) 79, 302, 404, 836, 1079
NaeI GCCGGC 1 cut(s) 1489
NciI CCSGG 1 cut(s) 355
NcoI CCATGG 1 cut(s) 40
NdeI CATATG 1 cut(s) 564
NdeII GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
NgoMIV GCCGGC 1 cut(s) 1487
NlaIII CATG 8 cut(s) 44, 65, 252, 343, 508, 561, 925, 1264
NlaIV GGNNCC 4 cut(s) 14, 359, 360, 1333
NmeAIII GCCGAG 1 cut(s) 1216
NmuCI GTSAC 6 cut(s) 103, 201, 364, 793, 952, 1183
OliI CACNNNNGTG 3 cut(s) 540, 959, 1162
PcsI WCGNNNNNNNCGW 1 cut(s) 172
PdiI GCCGGC 1 cut(s) 1489
PfeI GAWTC 5 cut(s) 33, 110, 597, 1041, 1341
PflFI GACNNNGTC 1 cut(s) 362
PkrI GCNGC 3 cut(s) 664, 997, 1471
PpuMI RGGWCCY 1 cut(s) 358
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 1015
PspGI CCWGG 1 cut(s) 1015
PspN4I GGNNCC 4 cut(s) 14, 359, 360, 1333
PspPI GGNCC 5 cut(s) 281, 351, 358, 868, 1257
PspPPI RGGWCCY 1 cut(s) 358
PstI CTGCAG 2 cut(s) 220, 910
PsuI RGATCY 4 cut(s) 227, 553, 896, 1501
PsyI GACNNNGTC 1 cut(s) 362
RsaI GTAC 4 cut(s) 320, 452, 1226, 1379
RsaNI GTAC 4 cut(s) 319, 451, 1225, 1378
RseI CAYNNNNRTG 4 cut(s) 540, 605, 959, 1162
SaqAI TTAA 2 cut(s) 738, 1028
SatI GCNGC 3 cut(s) 663, 996, 1470
Sau3AI GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
Sau96I GGNCC 5 cut(s) 281, 351, 358, 868, 1257
ScaI AGTACT 1 cut(s) 452
ScrFI CCNGG 2 cut(s) 355, 1017
SduI GDGCHC 1 cut(s) 1159
SfaNI GCATC 4 cut(s) 385, 500, 954, 971
SfcI CTRYAG 3 cut(s) 216, 801, 906
SgrAI CRCCGGYG 1 cut(s) 1487
SinI GGWCC 4 cut(s) 281, 358, 868, 1257
SmiMI CAYNNNNRTG 4 cut(s) 540, 605, 959, 1162
Sse9I AATT 7 cut(s) 255, 285, 379, 457, 474, 879, 1083
SspMI CTAG 4 cut(s) 570, 1064, 1097, 1499
StyD4I CCNGG 2 cut(s) 353, 1015
StyI CCWWGG 3 cut(s) 8, 40, 697
TaaI ACNGT 5 cut(s) 691, 772, 952, 961, 1357
TaiI ACGT 1 cut(s) 203
TasI AATT 7 cut(s) 255, 285, 379, 457, 474, 879, 1083
TatI WGTACW 3 cut(s) 318, 450, 1377
TfiI GAWTC 5 cut(s) 33, 110, 597, 1041, 1341
Tru1I TTAA 2 cut(s) 738, 1028
Tru9I TTAA 2 cut(s) 738, 1028
TscAI CASTG 5 cut(s) 112, 220, 694, 955, 966
TseFI GTSAC 6 cut(s) 103, 201, 364, 793, 952, 1183
TseI GCWGC 3 cut(s) 662, 995, 1469
Tsp45I GTSAC 6 cut(s) 103, 201, 364, 793, 952, 1183
TspDTI ATGAA 2 cut(s) 651, 938
TspRI CASTG 5 cut(s) 112, 220, 694, 955, 966
Tth111I GACNNNGTC 1 cut(s) 362
VneI GTGCAC 1 cut(s) 1155
VpaK11BI GGWCC 4 cut(s) 281, 358, 868, 1257
XapI RAATTY 2 cut(s) 457, 879
XbaI TCTAGA 2 cut(s) 1063, 1498
XspI CTAG 4 cut(s) 570, 1064, 1097, 1499
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.