Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0013945

Physical Location & Seq

Genomic Coordinates Forward (+)

10771128 .. 10773933

Gene Structure 2806 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGGCCACGTCAGCATCCCAAACCCTCCGCACCCCATTTCTCCGCAACCCTACTTCCTCGCTCTCTCTCTCCTCCAAGACCCCCCTCCCCTTCGCCCTCTCCCTCCCCTCCAGCCGCCGCCTCGCCTCGCCGAGACTCGTCGTGGTTCTCTCCGCGGCCAGGCTCGACGCCAAGCCGACGGTTTTGGTCGCCGAGAAGCTCGGCGACGCCGGGCTGGACCTGTTGAAGGAGTTCGCGAATGTGGACTGCTCGTATAATCTGAGCCCCGAGGAGCTCTGCACGAAGATCTCGCTCTGCGACGCGTTGATTGTCCGGAGCGGGACGAAGGTCAGCCGCGAGGTGTTTGAGTCCTCCGGCGGGAGGTTGAAGGTGGTCGGCAGAGCCGGCGTGGGGATTGATAATGTGGATCTGGGCGCCGCGACTGAGTTTGGTTGTTTGGTGGTTAATGCTCCGACGGCCAACACCGTCGCCGCGGCAGAGCATGGTATTGCTCTCCTCACCGCCATGGCTCGCAACGTGGCTCAGGCTGATGCTTCTGTTAAAGCTGGTAAATGGCAGCGAAACAAGTATGTGGGAGTTTCGCTTGTTGGGAAGACCCTGGCTGTGATGGGATTTGGAAAGGTTGGATCTGAAGTGGCTCGTCGTGCTAAGGGGCTTGGTATGCATGTGATTGCACACGATCCTTATGCCCCGGCAGATCGTGCTCGTGCCATAGGTGTAGAGCTTGTGAGCTTCGATGAGGCTTTGGCCACTGCGGATTTCATCTCCCTGCATATGCCTCTCACTGCTGCTACAAATAAGGTTCTCAACGACGAGACGTTTGCGAAGATGAAGAAAGGAGTGAGAATTGTGAATGTTGCACGTGGTGGTGTTATCGACGAAGATGCACTGGTCAAGGCACTTGATTCGGGAATTGTTGCTCAGGCTGCTCTTGATGTGTTTACTGTGGAGCCACCGCCACAGGGCAACAAGTTGGTGTTGCATGAGAGAGTTACTGCCACCCCTCATCTTGGTGCTAGTACCATGGAAGCACAGGAAGGAGTGGCCATTGAAATTGCTGAAGCCGTCGTTGGAGCCTTAAAAGGCGAGCTTGCTGCCACCGCAGTCAATGCACCAATGGTTCCCGCTGAGGTTTTGGCGGAACTGAAGCCTTATGTTGAACTTGCTGAGAAACTTGGGAGACTGGCTGTCCAGTTAGTGGCAGGAGGCAGTGGTGTGAAGAATGTAAAGGTTTCATATGCTTCAGCTAGAGCTCCTGATGACCTTGACACTAGACTTCTTCGAGCCATGATTACTAAGGGTCTCATTGAGCCAATATCCAGTGTTTTCGTGAATCTGGTCAATGCTGATTTCACTGCTAAACAGAGAGGACTCAGGATAACAGAAGAGCGGACCATTCTAGATGGTTCACCTGAGAGTCCCTTAGACTCCATCCAAGTTCAGATTGCCAATGTGGAATCCAAATTTGCCAGTGCCATATCAGACAGTGGGGAGATAAAGGTGGAGGGTCGGGTTAAGGATGGGGTTCCCCACTTGACGAGGGTTGGATCTTTTGAAGTTGATGTGAGCTTGGAAGGTAGTATTATACTCTGCAGACAGGTTGATCAGCCGGGTATGATAGGATTGGTTGGAAGCATTTTAGGAGAGGAGAATGTGAACGTTAGTTTCATGAGTGTCGGAAGAATTGCCCCAAGAAAGCAGGCTGTCATGGCAATTGGTGTGGATGACCAACCCAGCAAGCAATCTTTGAAAAGGATTGGGGATGTTCCAGCCATTGAAGAGTTTGTTTTCCTCAAGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000166 GO:0003006 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009553 GO:0009555 GO:0009561 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0016020 GO:0017076 GO:0022414 GO:0030554 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00260 ko00680 ko01100 ko01120 ko01130 ko01200 ko01230 map00260 map00680 map01100 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C ACT

Protein Analysis

600

Amino Acids

63.09

Weight (kDa)

6.45

Isoelectric Point (pI)

36.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	61 - 370	5e-33	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	163 - 338	6.9e-63	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
KARI_N	PF07991	195 - 359	7.4e-07	Acetohydroxy acid isomeroreductase, NADPH-binding domain
PGDH_inter	PF19304	381 - 494	3.2e-15	D-3-phosphoglycerate dehydrogenase intervening domain
ACT	PF01842	528 - 589	2.5e-06	ACT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19480 AT4G34200
fragaria_vesca	FvH4_2g05310
malus_domestica	MD05G1088400.v1.1
prunus_persica	Prupe.8G134300_v2.0.a1 Prupe.8G134300_v2.0.a1
pyrus_communis	pycom05g08770
rosa_chinensis	RchiOBHm_Chr6g0255451
rosa_laevigata	RLG00000014836
rosa_multiflora	Rmu_sc0002360.1_g000009
rosa_roxburghii	Rroxscaffold_7G00209970
rosa_rugosa	Rorug05G0558900
rosa_samantha	Rh6AG074800 Rh6BG069400 Rh6CG068400 Rh6DG065200
rosa_wichuraiana	Rw6G006650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	413
AccB7I	CCANNNNNTGG	1 cut(s)	1198
AccBSI	CCGCTC	2 cut(s)	318, 1390
AccII	CGCG	6 cut(s)	155, 236, 302, 336, 419, 473
AccIII	TCCGGA	1 cut(s)	312
AclI	AACGTT	1 cut(s)	1659
AclWI	GGATC	4 cut(s)	414, 634, 674, 1555
AcoI	YGGCCR	5 cut(s)	3, 156, 456, 747, 1044
AcsI	RAATTY	1 cut(s)	1463
AcuI	CTGAAG	4 cut(s)	651, 1080, 1166, 1227
AcvI	CACGTG	1 cut(s)	863
AcyI	GRCGYC	3 cut(s)	168, 207, 414
AdeI	CACNNNGTG	1 cut(s)	866
AfaI	GTAC	1 cut(s)	1021
AfiI	CCNNNNNNNGG	8 cut(s)	159, 226, 357, 360, 390, 962, 1010, 1198
AflIII	ACRYGT	1 cut(s)	300
AgsI	TTSAA	7 cut(s)	226, 367, 1052, 1160, 1556, 1750, 1778
AhdI	GACNNNNNGTC	1 cut(s)	1187
AjiI	CACGTC	1 cut(s)	9
AjnI	CCWGG	2 cut(s)	158, 597
AjuI	GAANNNNNNNTTGG	2 cut(s)	1053, 1085
AluBI	AGCT	9 cut(s)	199, 274, 545, 724, 732, 1090, 1247, 1253, 1569
AluI	AGCT	9 cut(s)	199, 274, 545, 724, 732, 1090, 1247, 1253, 1569
Alw21I	GWGCWC	3 cut(s)	276, 706, 1255
Alw26I	GTCTC	4 cut(s)	127, 809, 1174, 1307
AlwI	GGATC	4 cut(s)	414, 634, 674, 1555
Ama87I	CYCGRG	1 cut(s)	266
Aor13HI	TCCGGA	1 cut(s)	312
AoxI	GGCC	5 cut(s)	3, 156, 456, 747, 1044
ApeKI	GCWGC	4 cut(s)	556, 788, 926, 1094
ApoI	RAATTY	1 cut(s)	1463
ArsI	GACNNNNNNTTYG	2 cut(s)	803, 835
Asp700I	GAANNNNTTC	1 cut(s)	230
AspLEI	GCGC	1 cut(s)	416
AspS9I	GGNCC	2 cut(s)	217, 1392
AsuC2I	CCSGG	3 cut(s)	211, 692, 1611
AsuHPI	GGTGA	2 cut(s)	490, 1401
AvaI	CYCGRG	1 cut(s)	266
AvaII	GGWCC	2 cut(s)	217, 1392
BalI	TGGCCA	3 cut(s)	5, 749, 1046
BanI	GGYRCC	1 cut(s)	413
BanII	GRGCYC	3 cut(s)	266, 276, 1255
BauI	CACGAG	1 cut(s)	705
BbrPI	CACGTG	1 cut(s)	863
BbsI	GAAGAC	1 cut(s)	599
Bbv12I	GWGCWC	3 cut(s)	276, 706, 1255
BbvCI	CCTCAGC	1 cut(s)	1128
BbvI	GCAGC	4 cut(s)	568, 775, 913, 1081
BccI	CCATC	4 cut(s)	601, 1397, 1439, 1514
BceAI	ACGGC	2 cut(s)	471, 1049
BciT130I	CCWGG	2 cut(s)	160, 599
BclI	TGATCA	1 cut(s)	1603
BcnI	CCSGG	3 cut(s)	211, 692, 1611
BcoDI	GTCTC	4 cut(s)	127, 809, 1174, 1307
BfaI	CTAG	4 cut(s)	1017, 1248, 1272, 1400
BfmI	CTRYAG	1 cut(s)	1591
BfoI	RGCGCY	1 cut(s)	417
BglII	AGATCT	1 cut(s)	285
Bme1390I	CCNGG	5 cut(s)	160, 211, 599, 692, 1611
Bme18I	GGWCC	2 cut(s)	217, 1392
BmeRI	GACNNNNNGTC	1 cut(s)	1187
BmeT110I	CYCGRG	1 cut(s)	266
BmgBI	CACGTC	1 cut(s)	9
BmgT120I	GGNCC	2 cut(s)	217, 1392
BmiI	GGNNCC	5 cut(s)	415, 951, 1075, 1122, 1527
BmrFI	CCNGG	5 cut(s)	160, 211, 599, 692, 1611
BmsI	GCATC	3 cut(s)	23, 520, 874
BoxI	GACNNNNGTC	1 cut(s)	326
BpiI	GAAGAC	1 cut(s)	599
BpmI	CTGGAG	1 cut(s)	94
Bpu10I	CCTNAGC	4 cut(s)	522, 648, 921, 1128
BpuEI	CTTGAG	1 cut(s)	1778
BpuMI	CCSGG	3 cut(s)	211, 692, 1611
BsaAI	YACGTR	1 cut(s)	863
BsaHI	GRCGYC	3 cut(s)	168, 207, 414
BsaI	GGTCTC	1 cut(s)	1307
BsaJI	CCNNGG	7 cut(s)	153, 267, 471, 504, 597, 690, 1023
BsaWI	WCCGGW	1 cut(s)	312
Bsc4I	CCNNNNNNNGG	8 cut(s)	159, 226, 357, 360, 390, 962, 1010, 1198
Bse118I	RCCGGY	1 cut(s)	383
Bse1I	ACTGG	5 cut(s)	894, 1188, 1192, 1320, 1470
BseAI	TCCGGA	1 cut(s)	312
BseBI	CCWGG	2 cut(s)	160, 599
BseDI	CCNNGG	7 cut(s)	153, 267, 471, 504, 597, 690, 1023
BseGI	GGATG	5 cut(s)	14, 1431, 1525, 1729, 1768
BseLI	CCNNNNNNNGG	8 cut(s)	159, 226, 357, 360, 390, 962, 1010, 1198
BseMII	CTCAG	8 cut(s)	251, 414, 536, 935, 1119, 1158, 1387, 1404
BseNI	ACTGG	5 cut(s)	894, 1188, 1192, 1320, 1470
BseRI	GAGGAG	4 cut(s)	61, 284, 485, 1661
BseXI	GCAGC	4 cut(s)	568, 775, 913, 1081
BseYI	CCCAGC	1 cut(s)	1733
BsgI	GTGCAG	1 cut(s)	262
Bsh1236I	CGCG	6 cut(s)	155, 236, 302, 336, 419, 473
BshFI	GGCC	5 cut(s)	5, 158, 458, 749, 1046
BshNI	GGYRCC	1 cut(s)	413
BsiHKAI	GWGCWC	3 cut(s)	276, 706, 1255
BsiHKCI	CYCGRG	1 cut(s)	266
BsiSI	CCGG	6 cut(s)	210, 313, 354, 384, 692, 1610
BslFI	GGGAC	2 cut(s)	334, 1404
BslI	CCNNNNNNNGG	8 cut(s)	159, 226, 357, 360, 390, 962, 1010, 1198
BsmAI	GTCTC	4 cut(s)	127, 809, 1174, 1307
BsmBI	CGTCTC	1 cut(s)	809
BsmFI	GGGAC	2 cut(s)	334, 1404
BsnI	GGCC	5 cut(s)	5, 158, 458, 749, 1046
Bso31I	GGTCTC	1 cut(s)	1307
BsoBI	CYCGRG	1 cut(s)	266
Bsp1286I	GDGCHC	4 cut(s)	266, 276, 706, 1255
Bsp13I	TCCGGA	1 cut(s)	312
Bsp143I	GATC	7 cut(s)	285, 406, 626, 679, 697, 1547, 1603
Bsp19I	CCATGG	2 cut(s)	504, 1023
Bsp68I	TCGCGA	1 cut(s)	236
BspANI	GGCC	5 cut(s)	5, 158, 458, 749, 1046
BspCNI	CTCAG	8 cut(s)	252, 415, 535, 934, 1120, 1159, 1386, 1405
BspEI	TCCGGA	1 cut(s)	312
BspFNI	CGCG	6 cut(s)	155, 236, 302, 336, 419, 473
BspHI	TCATGA	1 cut(s)	1668
BspLI	GGNNCC	5 cut(s)	415, 951, 1075, 1122, 1527
BspMAI	CTGCAG	1 cut(s)	1595
BspPI	GGATC	4 cut(s)	414, 634, 674, 1555
BspQI	GCTCTTC	1 cut(s)	1380
BspT107I	GGYRCC	1 cut(s)	413
BspTNI	GGTCTC	1 cut(s)	1307
BsrBI	CCGCTC	2 cut(s)	318, 1390
BsrFI	RCCGGY	1 cut(s)	383
BsrI	ACTGG	5 cut(s)	894, 1188, 1192, 1320, 1470
BssAI	RCCGGY	1 cut(s)	383
BssECI	CCNNGG	7 cut(s)	153, 267, 471, 504, 597, 690, 1023
BssMI	GATC	7 cut(s)	285, 406, 626, 679, 697, 1547, 1603
BssNI	GRCGYC	3 cut(s)	168, 207, 414
BssSI	CACGAG	1 cut(s)	705
BssT1I	CCWWGG	2 cut(s)	504, 1023
Bst2BI	CACGAG	1 cut(s)	705
Bst2UI	CCWGG	2 cut(s)	160, 599
Bst4CI	ACNGT	4 cut(s)	181, 466, 946, 1487
Bst6I	CTCTTC	2 cut(s)	1380, 1773
BstACI	GRCGYC	3 cut(s)	168, 207, 414
BstAPI	GCANNNNNTGC	2 cut(s)	701, 1109
BstBAI	YACGTR	1 cut(s)	863
BstC8I	GCNNGC	6 cut(s)	385, 511, 1088, 1092, 1701, 1739
BstDSI	CCRYGG	4 cut(s)	153, 471, 504, 1023
BstENI	CCTNNNNNAGG	1 cut(s)	224
BstF5I	GGATG	5 cut(s)	14, 1431, 1525, 1729, 1768
BstFNI	CGCG	6 cut(s)	155, 236, 302, 336, 419, 473
BstH2I	RGCGCY	1 cut(s)	417
BstHHI	GCGC	1 cut(s)	416
BstKTI	GATC	7 cut(s)	288, 409, 629, 682, 700, 1550, 1606
BstMAI	GTCTC	4 cut(s)	127, 809, 1174, 1307
BstMBI	GATC	7 cut(s)	285, 406, 626, 679, 697, 1547, 1603
BstNI	CCWGG	2 cut(s)	160, 599
BstNSI	RCATGY	1 cut(s)	668
BstPAI	GACNNNNGTC	1 cut(s)	326
BstSCI	CCNGG	5 cut(s)	158, 209, 597, 690, 1609
BstSFI	CTRYAG	1 cut(s)	1591
BstUI	CGCG	6 cut(s)	155, 236, 302, 336, 419, 473
BstV1I	GCAGC	4 cut(s)	568, 775, 913, 1081
BstV2I	GAAGAC	1 cut(s)	599
BstX2I	RGATCY	4 cut(s)	285, 406, 626, 1547
BstYI	RGATCY	4 cut(s)	285, 406, 626, 1547
BsuRI	GGCC	5 cut(s)	5, 158, 458, 749, 1046
BtgI	CCRYGG	4 cut(s)	153, 471, 504, 1023
BtrI	CACGTC	1 cut(s)	9
BtsCI	GGATG	5 cut(s)	14, 1431, 1525, 1729, 1768
BtsI	GCAGTG	4 cut(s)	750, 783, 1216, 1353
BtsIMutI	CAGTG	8 cut(s)	750, 783, 887, 1216, 1327, 1353, 1477, 1492
BtuMI	TCGCGA	1 cut(s)	236
Cac8I	GCNNGC	6 cut(s)	385, 511, 1088, 1092, 1701, 1739
CciI	TCATGA	1 cut(s)	1668
CfoI	GCGC	1 cut(s)	416
Cfr10I	RCCGGY	1 cut(s)	383
Cfr13I	GGNCC	2 cut(s)	217, 1392
Cfr42I	CCGCGG	2 cut(s)	156, 474
CseI	GACGC	3 cut(s)	176, 215, 308
Csp6I	GTAC	1 cut(s)	1020
CspCI	CAANNNNNGTGG	2 cut(s)	1701, 1736
CviAII	CATG	8 cut(s)	482, 505, 665, 983, 1024, 1288, 1669, 1708
CviQI	GTAC	1 cut(s)	1020
DinI	GGCGCC	1 cut(s)	415
DpnI	GATC	7 cut(s)	287, 408, 628, 681, 699, 1549, 1605
DpnII	GATC	7 cut(s)	285, 406, 626, 679, 697, 1547, 1603
DraIII	CACNNNGTG	1 cut(s)	866
DriI	GACNNNNNGTC	1 cut(s)	1187
EaeI	YGGCCR	5 cut(s)	3, 156, 456, 747, 1044
Eam1104I	CTCTTC	2 cut(s)	1380, 1773
Eam1105I	GACNNNNNGTC	1 cut(s)	1187
EarI	CTCTTC	2 cut(s)	1380, 1773
EciI	GGCGGA	1 cut(s)	1154
Ecl136II	GAGCTC	2 cut(s)	274, 1253
Eco130I	CCWWGG	2 cut(s)	504, 1023
Eco24I	GRGCYC	3 cut(s)	266, 276, 1255
Eco31I	GGTCTC	1 cut(s)	1307
Eco47I	GGWCC	2 cut(s)	217, 1392
Eco53kI	GAGCTC	2 cut(s)	274, 1253
Eco57I	CTGAAG	4 cut(s)	651, 1080, 1166, 1227
Eco72I	CACGTG	1 cut(s)	863
Eco88I	CYCGRG	1 cut(s)	266
EcoICRI	GAGCTC	2 cut(s)	274, 1253
EcoNI	CCTNNNNNAGG	1 cut(s)	224
EcoRII	CCWGG	2 cut(s)	158, 597
EcoT14I	CCWWGG	2 cut(s)	504, 1023
EcoT22I	ATGCAT	1 cut(s)	666
EcoT38I	GRGCYC	3 cut(s)	266, 276, 1255
EgeI	GGCGCC	1 cut(s)	415
EheI	GGCGCC	1 cut(s)	415
ErhI	CCWWGG	2 cut(s)	504, 1023
Esp3I	CGTCTC	1 cut(s)	809
FaeI	CATG	8 cut(s)	485, 508, 668, 986, 1027, 1291, 1672, 1711
FalI	AAGNNNNNCTT	2 cut(s)	1074, 1106
FaqI	GGGAC	2 cut(s)	334, 1404
FatI	CATG	8 cut(s)	481, 504, 664, 982, 1023, 1287, 1668, 1707
FauI	CCCGC	3 cut(s)	311, 350, 1132
FauNDI	CATATG	2 cut(s)	774, 1237
FbaI	TGATCA	1 cut(s)	1603
FokI	GGATG	4 cut(s)	1418, 1532, 1736, 1775
FriOI	GRGCYC	3 cut(s)	266, 276, 1255
FspBI	CTAG	4 cut(s)	1017, 1248, 1272, 1400
GlaI	GCGC	1 cut(s)	415
GsaI	CCCAGC	1 cut(s)	1737
GsuI	CTGGAG	1 cut(s)	94
HaeII	RGCGCY	1 cut(s)	417
HaeIII	GGCC	5 cut(s)	5, 158, 458, 749, 1046
HapII	CCGG	6 cut(s)	210, 313, 354, 384, 692, 1610
HgaI	GACGC	3 cut(s)	176, 215, 308
HhaI	GCGC	1 cut(s)	416
Hin1I	GRCGYC	3 cut(s)	168, 207, 414
Hin1II	CATG	8 cut(s)	485, 508, 668, 986, 1027, 1291, 1672, 1711
Hin6I	GCGC	1 cut(s)	414
HinP1I	GCGC	1 cut(s)	414
HinfI	GANTC	8 cut(s)	135, 347, 905, 1333, 1371, 1417, 1427, 1457
HpaII	CCGG	6 cut(s)	210, 313, 354, 384, 692, 1610
HphI	GGTGA	2 cut(s)	490, 1401
Hpy166II	GTNNAC	4 cut(s)	244, 942, 1409, 1657
Hpy188I	TCNGA	6 cut(s)	261, 453, 631, 1443, 1483, 1679
Hpy188III	TCNNGA	9 cut(s)	235, 313, 909, 932, 1256, 1330, 1375, 1400, 1669
Hpy8I	GTNNAC	4 cut(s)	244, 942, 1409, 1657
HpyAV	CCTTC	6 cut(s)	100, 220, 319, 361, 1031, 1568
HpyCH4III	ACNGT	4 cut(s)	181, 466, 946, 1487
HpyCH4IV	ACGT	5 cut(s)	8, 516, 818, 862, 1659
HpyCH4V	TGCA	9 cut(s)	279, 664, 674, 772, 860, 887, 982, 1112, 1593
HpySE526I	ACGT	5 cut(s)	8, 516, 818, 862, 1659
Hsp92I	GRCGYC	3 cut(s)	168, 207, 414
Hsp92II	CATG	8 cut(s)	485, 508, 668, 986, 1027, 1291, 1672, 1711
HspAI	GCGC	1 cut(s)	414
KasI	GGCGCC	1 cut(s)	413
Kpn2I	TCCGGA	1 cut(s)	312
KroI	GCCGGC	1 cut(s)	383
KroNI	GCCGGC	1 cut(s)	385
Ksp22I	TGATCA	1 cut(s)	1603
KspI	CCGCGG	2 cut(s)	156, 474
Kzo9I	GATC	7 cut(s)	285, 406, 626, 679, 697, 1547, 1603
LguI	GCTCTTC	1 cut(s)	1380
LmnI	GCTCC	6 cut(s)	271, 315, 454, 949, 1073, 1258
Lsp1109I	GCAGC	4 cut(s)	568, 775, 913, 1081
LweI	GCATC	3 cut(s)	23, 520, 874
MaeI	CTAG	4 cut(s)	1017, 1248, 1272, 1400
MaeII	ACGT	5 cut(s)	8, 516, 818, 862, 1659
MaeIII	GTNAC	1 cut(s)	991
MalI	GATC	7 cut(s)	287, 408, 628, 681, 699, 1549, 1605
MbiI	CCGCTC	2 cut(s)	318, 1390
MboI	GATC	7 cut(s)	285, 406, 626, 679, 697, 1547, 1603
MfeI	CAATTG	1 cut(s)	1713
MflI	RGATCY	4 cut(s)	285, 406, 626, 1547
MhlI	GDGCHC	4 cut(s)	266, 276, 706, 1255
MlsI	TGGCCA	3 cut(s)	5, 749, 1046
MluCI	AATT	6 cut(s)	846, 912, 1053, 1463, 1683, 1713
MluI	ACGCGT	1 cut(s)	300
MluNI	TGGCCA	3 cut(s)	5, 749, 1046
Mly113I	GGCGCC	1 cut(s)	414
MlyI	GAGTC	5 cut(s)	129, 356, 1365, 1421, 1426
MmeI	TCCRAC	6 cut(s)	476, 604, 1051, 1525, 1609, 1657
Mox20I	TGGCCA	3 cut(s)	5, 749, 1046
Mph1103I	ATGCAT	1 cut(s)	666
MroI	TCCGGA	1 cut(s)	312
MroNI	GCCGGC	1 cut(s)	383
MroXI	GAANNNNTTC	1 cut(s)	230
MscI	TGGCCA	3 cut(s)	5, 749, 1046
MseI	TTAA	4 cut(s)	444, 540, 1079, 1515
MslI	CAYNNNNRTG	2 cut(s)	503, 1011
Msp20I	TGGCCA	3 cut(s)	5, 749, 1046
MspA1I	CMGCKG	3 cut(s)	155, 473, 1127
MspI	CCGG	6 cut(s)	210, 313, 354, 384, 692, 1610
MspR9I	CCNGG	5 cut(s)	160, 211, 599, 692, 1611
MunI	CAATTG	1 cut(s)	1713
MvaI	CCWGG	2 cut(s)	160, 599
MvnI	CGCG	6 cut(s)	155, 236, 302, 336, 419, 473
NaeI	GCCGGC	1 cut(s)	385
NarI	GGCGCC	1 cut(s)	414
NciI	CCSGG	3 cut(s)	211, 692, 1611
NcoI	CCATGG	2 cut(s)	504, 1023
NdeI	CATATG	2 cut(s)	774, 1237
NdeII	GATC	7 cut(s)	285, 406, 626, 679, 697, 1547, 1603
NgoMIV	GCCGGC	1 cut(s)	383
NlaIII	CATG	8 cut(s)	485, 508, 668, 986, 1027, 1291, 1672, 1711
NlaIV	GGNNCC	5 cut(s)	415, 951, 1075, 1122, 1527
NmeAIII	GCCGAG	3 cut(s)	156, 180, 217
NruI	TCGCGA	1 cut(s)	236
NsiI	ATGCAT	1 cut(s)	666
NspI	RCATGY	1 cut(s)	668
PagI	TCATGA	1 cut(s)	1668
PciSI	GCTCTTC	1 cut(s)	1380
PdiI	GCCGGC	1 cut(s)	385
PdmI	GAANNNNTTC	1 cut(s)	230
PfeI	GAWTC	3 cut(s)	905, 1333, 1457
PflMI	CCANNNNNTGG	1 cut(s)	1198
PleI	GAGTC	5 cut(s)	129, 355, 1365, 1421, 1425
PluTI	GGCGCC	1 cut(s)	417
PmaCI	CACGTG	1 cut(s)	863
PmlI	CACGTG	1 cut(s)	863
PpsI	GAGTC	5 cut(s)	129, 355, 1365, 1421, 1425
Ppu21I	YACGTR	1 cut(s)	863
PshAI	GACNNNNGTC	1 cut(s)	326
Psp124BI	GAGCTC	2 cut(s)	276, 1255
Psp1406I	AACGTT	1 cut(s)	1659
Psp6I	CCWGG	2 cut(s)	158, 597
PspCI	CACGTG	1 cut(s)	863
PspFI	CCCAGC	1 cut(s)	1733
PspGI	CCWGG	2 cut(s)	158, 597
PspN4I	GGNNCC	5 cut(s)	415, 951, 1075, 1122, 1527
PspPI	GGNCC	2 cut(s)	217, 1392
PstI	CTGCAG	1 cut(s)	1595
PsuI	RGATCY	4 cut(s)	285, 406, 626, 1547
RruI	TCGCGA	1 cut(s)	236
RsaI	GTAC	1 cut(s)	1021
RsaNI	GTAC	1 cut(s)	1020
RseI	CAYNNNNRTG	2 cut(s)	503, 1011
SacI	GAGCTC	2 cut(s)	276, 1255
SacII	CCGCGG	2 cut(s)	156, 474
SapI	GCTCTTC	1 cut(s)	1380
SaqAI	TTAA	4 cut(s)	444, 540, 1079, 1515
Sau3AI	GATC	7 cut(s)	285, 406, 626, 679, 697, 1547, 1603
Sau96I	GGNCC	2 cut(s)	217, 1392
SchI	GAGTC	5 cut(s)	129, 356, 1365, 1421, 1426
ScrFI	CCNGG	5 cut(s)	160, 211, 599, 692, 1611
SduI	GDGCHC	4 cut(s)	266, 276, 706, 1255
SfaNI	GCATC	3 cut(s)	23, 520, 874
SfcI	CTRYAG	1 cut(s)	1591
SfoI	GGCGCC	1 cut(s)	415
Sfr303I	CCGCGG	2 cut(s)	156, 474
SgrBI	CCGCGG	2 cut(s)	156, 474
SinI	GGWCC	2 cut(s)	217, 1392
SmiMI	CAYNNNNRTG	2 cut(s)	503, 1011
SmlI	CTYRAG	1 cut(s)	1793
SmoI	CTYRAG	1 cut(s)	1793
Sse9I	AATT	6 cut(s)	846, 912, 1053, 1463, 1683, 1713
SspDI	GGCGCC	1 cut(s)	413
SspMI	CTAG	4 cut(s)	1017, 1248, 1272, 1400
SstI	GAGCTC	2 cut(s)	276, 1255
StyD4I	CCNGG	5 cut(s)	158, 209, 597, 690, 1609
StyI	CCWWGG	2 cut(s)	504, 1023
TaaI	ACNGT	4 cut(s)	181, 466, 946, 1487
TaiI	ACGT	5 cut(s)	11, 519, 821, 865, 1662
TaqI	TCGA	4 cut(s)	165, 735, 876, 1282
TasI	AATT	6 cut(s)	846, 912, 1053, 1463, 1683, 1713
TauI	GCSGC	7 cut(s)	117, 120, 158, 336, 419, 473, 476
TfiI	GAWTC	3 cut(s)	905, 1333, 1457
Tru1I	TTAA	4 cut(s)	444, 540, 1079, 1515
Tru9I	TTAA	4 cut(s)	444, 540, 1079, 1515
TscAI	CASTG	8 cut(s)	757, 790, 894, 1216, 1327, 1360, 1477, 1492
TseI	GCWGC	4 cut(s)	556, 788, 926, 1094
TspDTI	ATGAA	4 cut(s)	751, 845, 1224, 1657
TspRI	CASTG	8 cut(s)	757, 790, 894, 1216, 1327, 1360, 1477, 1492
Van91I	CCANNNNNTGG	1 cut(s)	1198
VpaK11BI	GGWCC	2 cut(s)	217, 1392
XagI	CCTNNNNNAGG	1 cut(s)	224
XapI	RAATTY	1 cut(s)	1463
XbaI	TCTAGA	1 cut(s)	1399
XceI	RCATGY	1 cut(s)	668
XmnI	GAANNNNTTC	1 cut(s)	230
XspI	CTAG	4 cut(s)	1017, 1248, 1272, 1400
Zsp2I	ATGCAT	1 cut(s)	666

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.