RchiOBHm_Chr6g0262941

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0016351
Physical Location & Seq
Reverse (-)
17715062 .. 17717140
2079 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1404 bp
ATGGAGCTACCCCCGGGCCTTGCATCCGACGAAGCTAGCCCCGAATGGTTTAGCAAGGCTGACAACGCATGGCAGCTCACTGCTGCTACACTTGTAGGCATGCAGAGTGTGCCCGGCCTTATCATCTTGTATGGAAGCATAGTCAAGAAGAAGTGGGCAGTCAACTCGGCCTTCATGGCCATGTACGCCTTTGCAACTGTCTTGGTTTGTTGGGTGGGTTGGGGCTACCAAATGTCATTCGGTACCGAATTGTTCACCTTCCTGGGACGCCCCAACGTGGCCTTGGATGAGAAGTACCTATTTACAAAAGCATTCTCCGGGAAATTGCCGAACGCGACCATGATTTATTTTCAGTTTGTGTTTGCAGCAATCACGTTGATTTTGATTGCGGGGGCGTTGTTGGGGAGGATGAATTTCCATGCGTGGATGTTGTTCGTACCACTTTGGGTGACATTTTCGTATCCGATAACCGCTTATAGTATTTGGAATCCAAATGGATGGTTGGCAAAGATGGGTCTTATTGATTATTCTGGAGGGTATGTCATTCACCTTTCTTCAGGAGTTGCTGGTTTCACTGCCGCTTATTGGGTCGGACCTAGAGCAACTAGTGACAGGGAGAGATTCCCTCCAAACAACATCCTGCTTATGTTGGCAGGAGCTGGACTACTTTGGATGGGATGGACTGGATTCAATGGTGGAGATCCCTACAGTGTGAGCATGGACGCATCTCTGGCCGTCCTCAACACCCATGTATGTACTGCCTCTAGCTTGCTGACGTGGCTCGTCCTTGACATTATTTTCTTTGGGAAGCCCTCTGTGATTGGAGCCACACAGGGGATGATCACTGGCTTGGTTTGCATAACTCCTGCTGCGGTGGTGCAAGGTTGGGCAGCAATCATAATGGGAATCATGTCTGGAAGCATCCCATGGTACACAATGATGGTCCTCCACAAGCAAAGCAAGCTCCTGAAGCATGTAGACGACACCATGGCCGTGTTCCACACCCACGCTGTGGCCGGAAGCTTGGGTGGTCTCCTTACCGGCTTCTTTTCCAATCCCAAGCTGTGTCGTCTTTTCTATCTAGTTGACAACTGGCAACACTACATCGGTCTTTCCTATGGCATCCACGATGGTCGAGTTATGGCAGGGATGAAGCAAATGGGAATTCAACTTCTGGGAATCGTGTTTGTGGTTGTCGTGAACGTTATCATAACCAGCTTAATCTGTTTGCTGTTGAGGCTCATCGTTCCACTTAGGATGACAGAAGATGACTTGAAAATCGGGGATGATGCAATTCACGGGGAGGAGGCTTATGCATTGTGGGGAGATGGAGAGAAGTTTGATGGTTCCAAGCACAACTCAGTTTATGGCATGGATGACTTTGGTGTGGCAAGTAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

467

Amino Acids

51.05

Weight (kDa)

6.4

Isoelectric Point (pI)

27.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 23 - 438 2.1e-80 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016351)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30820
malus_domestica MD11G1277400.v1.1
prunus_persica Prupe.8G233700_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262941
rosa_laevigata RLG00000014406
rosa_multiflora Rmu_sc0000596.1_g000063 Rmu_sc0006493.1_g000013
rosa_roxburghii Rroxscaffold_177G00434070 Rroxscaffold_7G00204870
rosa_rugosa Rorug06G0001700
rosa_samantha Rh6AG121000 Rh6BG114700 Rh6CG113100 Rh6DG102400
rosa_wichuraiana Rw6G010390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 1012
AccI GTMKAC 1 cut(s) 978
AccII CGCG 1 cut(s) 335
AciI CCGC 4 cut(s) 389, 471, 579, 872
AclI AACGTT 1 cut(s) 1203
AclWI GGATC 1 cut(s) 695
AcoI YGGCCR 4 cut(s) 177, 732, 990, 1014
AcsI RAATTY 2 cut(s) 412, 1164
AcuI CTGAAG 2 cut(s) 540, 989
AcyI GRCGYC 1 cut(s) 268
AdeI CACNNNGTG 1 cut(s) 1012
AfaI GTAC 6 cut(s) 185, 244, 296, 438, 757, 932
AfiI CCNNNNNNNGG 4 cut(s) 277, 585, 834, 1012
AgsI TTSAA 3 cut(s) 691, 1169, 1276
AhlI ACTAGT 1 cut(s) 605
AjiI CACGTC 1 cut(s) 777
AjnI CCWGG 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 1037
AlwI GGATC 1 cut(s) 695
AlwNI CAGNNNCTG 1 cut(s) 659
Ama87I CYCGRG 1 cut(s) 13
AoxI GGCC 8 cut(s) 16, 115, 168, 177, 279, 732, 990, 1014
ApeKI GCWGC 5 cut(s) 73, 83, 365, 869, 890
ApoI RAATTY 2 cut(s) 412, 1164
Asp718I GGTACC 1 cut(s) 242
AspS9I GGNCC 3 cut(s) 16, 593, 943
AsuC2I CCSGG 4 cut(s) 14, 15, 114, 319
AsuHPI GGTGA 3 cut(s) 247, 460, 539
AsuNHI GCTAGC 1 cut(s) 35
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 2 cut(s) 593, 943
BaeGI GKGCMC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 1 cut(s) 242
BbvI GCAGC 5 cut(s) 70, 85, 377, 856, 902
BccI CCATC 8 cut(s) 492, 505, 667, 672, 934, 1124, 1322, 1337
BceAI ACGGC 2 cut(s) 719, 977
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BciT130I CCWGG 1 cut(s) 263
BciVI GTATCC 1 cut(s) 471
BclI TGATCA 1 cut(s) 840
BcnI CCSGG 4 cut(s) 14, 15, 114, 319
BcoDI GTCTC 1 cut(s) 1037
BcuI ACTAGT 1 cut(s) 605
BfaI CTAG 5 cut(s) 36, 597, 606, 765, 1082
BfmI CTRYAG 1 cut(s) 706
BfuI GTATCC 1 cut(s) 471
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 6 cut(s) 74, 84, 366, 579, 870, 891
BlsI GCNGC 6 cut(s) 75, 85, 367, 580, 871, 892
Bme1390I CCNGG 5 cut(s) 14, 15, 114, 263, 319
Bme18I GGWCC 2 cut(s) 593, 943
BmeT110I CYCGRG 1 cut(s) 13
BmgBI CACGTC 1 cut(s) 777
BmgT120I GGNCC 3 cut(s) 16, 593, 943
BmiI GGNNCC 3 cut(s) 244, 826, 1348
BmrFI CCNGG 5 cut(s) 14, 15, 114, 263, 319
BmsI GCATC 5 cut(s) 32, 734, 930, 1131, 1279
BmtI GCTAGC 1 cut(s) 39
BplI GAGNNNNNCTC 2 cut(s) 610, 642
BpmI CTGGAG 1 cut(s) 552
BpuMI CCSGG 4 cut(s) 14, 15, 114, 319
BsaHI GRCGYC 1 cut(s) 268
BsaI GGTCTC 1 cut(s) 1037
BsaJI CCNNGG 6 cut(s) 12, 13, 262, 282, 926, 987
BsaXI ACNNNNNCTCC 2 cut(s) 525, 555
Bsc4I CCNNNNNNNGG 4 cut(s) 277, 585, 834, 1012
Bse118I RCCGGY 1 cut(s) 1040
Bse1I ACTGG 3 cut(s) 688, 850, 1097
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 6 cut(s) 12, 13, 262, 282, 926, 987
BseLI CCNNNNNNNGG 4 cut(s) 277, 585, 834, 1012
BseMII CTCAG 1 cut(s) 1374
BseNI ACTGG 3 cut(s) 688, 850, 1097
BseRI GAGGAG 1 cut(s) 1319
BseSI GKGCMC 1 cut(s) 114
BseXI GCAGC 5 cut(s) 70, 85, 377, 856, 902
Bsh1236I CGCG 1 cut(s) 335
BshFI GGCC 8 cut(s) 18, 117, 170, 179, 281, 734, 992, 1016
BshNI GGYRCC 1 cut(s) 242
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 5 cut(s) 14, 114, 318, 1017, 1041
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 4 cut(s) 277, 585, 834, 1012
BsmAI GTCTC 1 cut(s) 1037
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 1 cut(s) 311
BsnI GGCC 8 cut(s) 18, 117, 170, 179, 281, 734, 992, 1016
Bso31I GGTCTC 1 cut(s) 1037
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 2 cut(s) 700, 840
Bsp19I CCATGG 2 cut(s) 926, 987
BspACI CCGC 4 cut(s) 389, 471, 579, 872
BspANI GGCC 8 cut(s) 18, 117, 170, 179, 281, 734, 992, 1016
BspCNI CTCAG 1 cut(s) 1373
BspFNI CGCG 1 cut(s) 335
BspLI GGNNCC 3 cut(s) 244, 826, 1348
BspOI GCTAGC 1 cut(s) 39
BspPI GGATC 1 cut(s) 695
BspT107I GGYRCC 1 cut(s) 242
BspTNI GGTCTC 1 cut(s) 1037
BsrFI RCCGGY 1 cut(s) 1040
BsrI ACTGG 3 cut(s) 688, 850, 1097
BssAI RCCGGY 1 cut(s) 1040
BssECI CCNNGG 6 cut(s) 12, 13, 262, 282, 926, 987
BssMI GATC 2 cut(s) 700, 840
BssNI GRCGYC 1 cut(s) 268
BssT1I CCWWGG 3 cut(s) 282, 926, 987
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 2 cut(s) 199, 710
BstACI GRCGYC 1 cut(s) 268
BstAPI GCANNNNNTGC 1 cut(s) 109
BstC8I GCNNGC 4 cut(s) 37, 101, 770, 962
BstDEI CTNAG 2 cut(s) 1253, 1360
BstDSI CCRYGG 2 cut(s) 926, 987
BstFNI CGCG 1 cut(s) 335
BstKTI GATC 2 cut(s) 703, 843
BstMAI GTCTC 1 cut(s) 1037
BstMBI GATC 2 cut(s) 700, 840
BstNI CCWGG 1 cut(s) 263
BstNSI RCATGY 2 cut(s) 103, 977
BstSCI CCNGG 5 cut(s) 12, 13, 112, 261, 317
BstSFI CTRYAG 1 cut(s) 706
BstSLI GKGCMC 1 cut(s) 114
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 5 cut(s) 70, 85, 377, 856, 902
BstX2I RGATCY 1 cut(s) 700
BstXI CCANNNNNNTGG 1 cut(s) 498
BstYI RGATCY 1 cut(s) 700
BsuI GTATCC 1 cut(s) 471
BsuRI GGCC 8 cut(s) 18, 117, 170, 179, 281, 734, 992, 1016
BtgI CCRYGG 2 cut(s) 926, 987
BtrI CACGTC 1 cut(s) 777
BtsI GCAGTG 2 cut(s) 78, 573
BtsIMutI CAGTG 4 cut(s) 78, 573, 715, 843
Cac8I GCNNGC 4 cut(s) 37, 101, 770, 962
CaiI CAGNNNCTG 1 cut(s) 659
Cfr10I RCCGGY 1 cut(s) 1040
Cfr13I GGNCC 3 cut(s) 16, 593, 943
Cfr9I CCCGGG 1 cut(s) 13
CseI GACGC 2 cut(s) 276, 731
Csp6I GTAC 6 cut(s) 184, 243, 295, 437, 756, 931
CviQI GTAC 6 cut(s) 184, 243, 295, 437, 756, 931
DdeI CTNAG 2 cut(s) 1253, 1360
DpnI GATC 2 cut(s) 702, 842
DpnII GATC 2 cut(s) 700, 840
DraIII CACNNNGTG 1 cut(s) 1012
EaeI YGGCCR 4 cut(s) 177, 732, 990, 1014
Eco130I CCWWGG 3 cut(s) 282, 926, 987
Eco31I GGTCTC 1 cut(s) 1037
Eco47I GGWCC 2 cut(s) 593, 943
Eco57I CTGAAG 2 cut(s) 540, 989
Eco88I CYCGRG 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 1164
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 3 cut(s) 282, 926, 987
EcoT22I ATGCAT 1 cut(s) 1318
ErhI CCWWGG 3 cut(s) 282, 926, 987
FalI AAGNNNNNCTT 1 cut(s) 1384
FaqI GGGAC 1 cut(s) 279
FauI CCCGC 1 cut(s) 382
FbaI TGATCA 1 cut(s) 840
FblI GTMKAC 1 cut(s) 978
Fnu4HI GCNGC 6 cut(s) 74, 84, 366, 579, 870, 891
Fsp4HI GCNGC 6 cut(s) 74, 84, 366, 579, 870, 891
FspBI CTAG 5 cut(s) 36, 597, 606, 765, 1082
GluI GCNGC 6 cut(s) 74, 84, 366, 579, 870, 891
GsuI CTGGAG 1 cut(s) 552
HaeIII GGCC 8 cut(s) 18, 117, 170, 179, 281, 734, 992, 1016
HapII CCGG 5 cut(s) 14, 114, 318, 1017, 1041
HgaI GACGC 2 cut(s) 276, 731
Hin1I GRCGYC 1 cut(s) 268
HincII GTYRAC 2 cut(s) 163, 1087
HindII GTYRAC 2 cut(s) 163, 1087
HindIII AAGCTT 2 cut(s) 1021, 1398
HinfI GANTC 5 cut(s) 487, 621, 687, 906, 1179
HpaII CCGG 5 cut(s) 14, 114, 318, 1017, 1041
HphI GGTGA 3 cut(s) 247, 460, 539
Hpy166II GTNNAC 6 cut(s) 163, 255, 933, 979, 1087, 1201
Hpy188I TCNGA 3 cut(s) 28, 465, 593
Hpy188III TCNNGA 6 cut(s) 145, 531, 558, 915, 967, 1198
Hpy8I GTNNAC 6 cut(s) 163, 255, 933, 979, 1087, 1201
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 181, 268
HpyCH4III ACNGT 2 cut(s) 199, 710
HpyCH4IV ACGT 4 cut(s) 276, 374, 776, 1203
HpyCH4V TGCA 8 cut(s) 23, 103, 194, 365, 858, 880, 1292, 1316
HpyF3I CTNAG 2 cut(s) 1253, 1360
HpySE526I ACGT 4 cut(s) 276, 374, 776, 1203
Hsp92I GRCGYC 1 cut(s) 268
KpnI GGTACC 1 cut(s) 246
Ksp22I TGATCA 1 cut(s) 840
Kzo9I GATC 2 cut(s) 700, 840
LmnI GCTCC 4 cut(s) 4, 656, 824, 969
Lsp1109I GCAGC 5 cut(s) 70, 85, 377, 856, 902
LweI GCATC 5 cut(s) 32, 734, 930, 1131, 1279
MaeI CTAG 5 cut(s) 36, 597, 606, 765, 1082
MaeII ACGT 4 cut(s) 276, 374, 776, 1203
MaeIII GTNAC 2 cut(s) 448, 608
MalI GATC 2 cut(s) 702, 842
MboI GATC 2 cut(s) 700, 840
MboII GAAGA 3 cut(s) 160, 546, 1277
MflI RGATCY 1 cut(s) 700
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 5 cut(s) 248, 323, 412, 1164, 1293
MluNI TGGCCA 1 cut(s) 179
MmeI TCCRAC 2 cut(s) 51, 571
Mox20I TGGCCA 1 cut(s) 179
Mph1103I ATGCAT 1 cut(s) 1318
MscI TGGCCA 1 cut(s) 179
MseI TTAA 2 cut(s) 1220, 1402
MslI CAYNNNNRTG 3 cut(s) 179, 938, 992
Msp20I TGGCCA 1 cut(s) 179
MspI CCGG 5 cut(s) 14, 114, 318, 1017, 1041
MspR9I CCNGG 5 cut(s) 14, 15, 114, 263, 319
Mva1269I GAATGC 1 cut(s) 311
MvaI CCWGG 1 cut(s) 263
MvnI CGCG 1 cut(s) 335
NciI CCSGG 4 cut(s) 14, 15, 114, 319
NcoI CCATGG 2 cut(s) 926, 987
NdeII GATC 2 cut(s) 700, 840
NheI GCTAGC 1 cut(s) 35
NlaIV GGNNCC 3 cut(s) 244, 826, 1348
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 2 cut(s) 448, 608
NsiI ATGCAT 1 cut(s) 1318
NspI RCATGY 2 cut(s) 103, 977
PaeI GCATGC 1 cut(s) 103
PctI GAATGC 1 cut(s) 311
PfeI GAWTC 5 cut(s) 487, 621, 687, 906, 1179
PflMI CCANNNNNTGG 1 cut(s) 1012
PfoI TCCNGGA 1 cut(s) 317
PkrI GCNGC 6 cut(s) 75, 85, 367, 580, 871, 892
Psp1406I AACGTT 1 cut(s) 1203
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 3 cut(s) 244, 826, 1348
PspPI GGNCC 3 cut(s) 16, 593, 943
PstNI CAGNNNCTG 1 cut(s) 659
PsuI RGATCY 1 cut(s) 700
RsaI GTAC 6 cut(s) 185, 244, 296, 438, 757, 932
RsaNI GTAC 6 cut(s) 184, 243, 295, 437, 756, 931
RseI CAYNNNNRTG 3 cut(s) 179, 938, 992
SaqAI TTAA 2 cut(s) 1220, 1402
SatI GCNGC 6 cut(s) 74, 84, 366, 579, 870, 891
Sau3AI GATC 2 cut(s) 700, 840
Sau96I GGNCC 3 cut(s) 16, 593, 943
ScrFI CCNGG 5 cut(s) 14, 15, 114, 263, 319
SduI GDGCHC 1 cut(s) 114
SfaNI GCATC 5 cut(s) 32, 734, 930, 1131, 1279
SfcI CTRYAG 1 cut(s) 706
SfiI GGCCNNNNNGGCC 1 cut(s) 176
SinI GGWCC 2 cut(s) 593, 943
SmaI CCCGGG 1 cut(s) 15
SmiMI CAYNNNNRTG 3 cut(s) 179, 938, 992
SpeI ACTAGT 1 cut(s) 605
SphI GCATGC 1 cut(s) 103
Sse9I AATT 5 cut(s) 248, 323, 412, 1164, 1293
SsiI CCGC 4 cut(s) 389, 471, 579, 872
SspMI CTAG 5 cut(s) 36, 597, 606, 765, 1082
StyD4I CCNGG 5 cut(s) 12, 13, 112, 261, 317
StyI CCWWGG 3 cut(s) 282, 926, 987
TaaI ACNGT 2 cut(s) 199, 710
TaiI ACGT 4 cut(s) 279, 377, 779, 1206
TaqI TCGA 1 cut(s) 1135
TaqII GACCGA 1 cut(s) 1097
TasI AATT 5 cut(s) 248, 323, 412, 1164, 1293
TatI WGTACW 1 cut(s) 755
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 5 cut(s) 487, 621, 687, 906, 1179
Tru1I TTAA 2 cut(s) 1220, 1402
Tru9I TTAA 2 cut(s) 1220, 1402
TscAI CASTG 4 cut(s) 85, 580, 715, 850
TseFI GTSAC 2 cut(s) 448, 608
TseI GCWGC 5 cut(s) 73, 83, 365, 869, 890
Tsp45I GTSAC 2 cut(s) 448, 608
TspDTI ATGAA 3 cut(s) 163, 425, 1166
TspMI CCCGGG 1 cut(s) 13
TspRI CASTG 4 cut(s) 85, 580, 715, 850
Van91I CCANNNNNTGG 1 cut(s) 1012
VpaK11BI GGWCC 2 cut(s) 593, 943
XapI RAATTY 2 cut(s) 412, 1164
XceI RCATGY 2 cut(s) 103, 977
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XmaI CCCGGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 978
XspI CTAG 5 cut(s) 36, 597, 606, 765, 1082
Zsp2I ATGCAT 1 cut(s) 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.