RchiOBHm_Chr6g0271961

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0016255
Physical Location & Seq
Forward (+)
31987933 .. 31988412
480 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 480 bp
ATGGGTATTTGTGCATCTACTCGGTGCTTGAAGGAAGGTGGAAGAATTGTATATGGTTCAAGCACAGTCAAGATGGTTCATTTGGATGGGAAGCTACAAGAGCTCAGGAGCCCAGTAAAAGCCTCCCACATTCTCTCACAGAATCCCAACTACTTCCTCTGCCCCTCGGAGTTCATGTTCGTCGACTCGGACGCCCCTCAAGTTCCTACCGATGAAGAGCTGCAGCTGGGTCAAATCTACTTCCTCATGCCAGTTTCTCAGCTAAGAGCACCACTGTCTCTCCAAGACTTGTGTTCACTTGCCATCAAGGCCAGTGTAGGTCTCGCAAAGGGTTCGAGCTTATTGGCGATGAGGTCGCCGGTTTCGGACAGAAATGTATCTGCATCACAGCTGGGATGTTGCAAGGTTCCAACTGGGTTTGACATGGTGGGATCAATGAGTTCAAGGATGAGGCGAGACAACCAAAGAATTGAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

159

Amino Acids

17.38

Weight (kDa)

8.58

Isoelectric Point (pI)

67.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 120 2.9e-26 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016255)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07760
malus_domestica MD10G1084100.v1.1
prunus_persica Prupe.8G107400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262091 RchiOBHm_Chr6g0271961
rosa_laevigata RLG00000014452
rosa_multiflora Rmu_sc0002510.1_g000009 Rmu_ssc0000125.1_g000022
rosa_roxburghii Rroxscaffold_7G00207830
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115100 Rh6BG109100 Rh6CG106500 Rh6DG097000
rosa_wichuraiana Rw6G009990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 183
AclWI GGATC 1 cut(s) 439
AcsI RAATTY 1 cut(s) 473
AcyI GRCGYC 1 cut(s) 192
AgsI TTSAA 4 cut(s) 31, 60, 444, 473
AloI GAACNNNNNNTCC 2 cut(s) 161, 193
AluBI AGCT 7 cut(s) 94, 103, 220, 226, 262, 339, 391
AluI AGCT 7 cut(s) 94, 103, 220, 226, 262, 339, 391
Alw21I GWGCWC 2 cut(s) 105, 271
Alw26I GTCTC 3 cut(s) 282, 326, 450
AlwI GGATC 1 cut(s) 439
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 2 cut(s) 220, 223
ApoI RAATTY 1 cut(s) 473
BanII GRGCYC 2 cut(s) 105, 113
Bbv12I GWGCWC 2 cut(s) 105, 271
BbvI GCAGC 2 cut(s) 207, 235
BccI CCATC 3 cut(s) 67, 80, 311
BcgI CGANNNNNNTGC 2 cut(s) 315, 349
BcoDI GTCTC 3 cut(s) 282, 326, 450
BfmI CTRYAG 1 cut(s) 221
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 2 cut(s) 221, 224
BlsI GCNGC 2 cut(s) 222, 225
BmiI GGNNCC 2 cut(s) 110, 408
BmrI ACTGGG 2 cut(s) 107, 423
BmsI GCATC 2 cut(s) 23, 392
BmuI ACTGGG 2 cut(s) 107, 423
Bpu10I CCTNAGC 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 192
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 264, 294
Bse118I RCCGGY 1 cut(s) 358
Bse1I ACTGG 4 cut(s) 113, 251, 312, 418
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 3 cut(s) 91, 401, 453
BseMII CTCAG 2 cut(s) 118, 272
BseNI ACTGG 4 cut(s) 113, 251, 312, 418
BseXI GCAGC 2 cut(s) 207, 235
BseYI CCCAGC 2 cut(s) 226, 391
BshFI GGCC 1 cut(s) 311
BsiHKAI GWGCWC 2 cut(s) 105, 271
BsiSI CCGG 1 cut(s) 359
BsmAI GTCTC 3 cut(s) 282, 326, 450
BsnI GGCC 1 cut(s) 311
Bso31I GGTCTC 1 cut(s) 326
Bsp1286I GDGCHC 3 cut(s) 105, 113, 271
Bsp143I GATC 1 cut(s) 431
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 2 cut(s) 117, 271
BspLI GGNNCC 2 cut(s) 110, 408
BspMAI CTGCAG 1 cut(s) 225
BspPI GGATC 1 cut(s) 439
BspQI GCTCTTC 1 cut(s) 210
BspTNI GGTCTC 1 cut(s) 326
BsrFI RCCGGY 1 cut(s) 358
BsrI ACTGG 4 cut(s) 113, 251, 312, 418
BssAI RCCGGY 1 cut(s) 358
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 1 cut(s) 431
BssNI GRCGYC 1 cut(s) 192
Bst4CI ACNGT 2 cut(s) 67, 276
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 192
BstDEI CTNAG 3 cut(s) 104, 258, 263
BstF5I GGATG 3 cut(s) 91, 401, 453
BstKTI GATC 1 cut(s) 434
BstMAI GTCTC 3 cut(s) 282, 326, 450
BstMBI GATC 1 cut(s) 431
BstMWI GCNNNNNNNGC 2 cut(s) 100, 308
BstSFI CTRYAG 1 cut(s) 221
BstV1I GCAGC 2 cut(s) 207, 235
BsuRI GGCC 1 cut(s) 311
BtgZI GCGATG 1 cut(s) 362
BtsCI GGATG 3 cut(s) 91, 401, 453
BtsIMutI CAGTG 2 cut(s) 272, 319
Cfr10I RCCGGY 1 cut(s) 358
CseI GACGC 1 cut(s) 200
CviAII CATG 3 cut(s) 175, 247, 424
DdeI CTNAG 3 cut(s) 104, 258, 263
DpnI GATC 1 cut(s) 433
DpnII GATC 1 cut(s) 431
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 103
Eco24I GRGCYC 2 cut(s) 105, 113
Eco31I GGTCTC 1 cut(s) 326
Eco53kI GAGCTC 1 cut(s) 103
EcoICRI GAGCTC 1 cut(s) 103
EcoT38I GRGCYC 2 cut(s) 105, 113
FaeI CATG 3 cut(s) 178, 250, 427
FaiI YATR 5 cut(s) 52, 54, 176, 248, 425
FatI CATG 3 cut(s) 174, 246, 423
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 2 cut(s) 221, 224
FokI GGATG 3 cut(s) 98, 408, 460
FriOI GRGCYC 2 cut(s) 105, 113
Fsp4HI GCNGC 2 cut(s) 221, 224
GluI GCNGC 2 cut(s) 221, 224
GsaI CCCAGC 2 cut(s) 230, 395
HaeIII GGCC 1 cut(s) 311
HapII CCGG 1 cut(s) 359
HgaI GACGC 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 3 cut(s) 178, 250, 427
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 2 cut(s) 142, 185
HpaII CCGG 1 cut(s) 359
Hpy166II GTNNAC 2 cut(s) 184, 296
Hpy188I TCNGA 3 cut(s) 169, 190, 367
Hpy188III TCNNGA 2 cut(s) 70, 106
Hpy8I GTNNAC 2 cut(s) 184, 296
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 25, 29
HpyCH4III ACNGT 2 cut(s) 67, 276
HpyCH4V TGCA 4 cut(s) 14, 223, 383, 402
HpyF10VI GCNNNNNNNGC 2 cut(s) 100, 308
HpyF3I CTNAG 3 cut(s) 104, 258, 263
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 3 cut(s) 178, 250, 427
Kzo9I GATC 1 cut(s) 431
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 1 cut(s) 108
LpnPI CCDG 8 cut(s) 91, 126, 212, 264, 325, 372, 377, 399
Lsp1109I GCAGC 2 cut(s) 207, 235
LweI GCATC 2 cut(s) 23, 392
MalI GATC 1 cut(s) 433
MboI GATC 1 cut(s) 431
MboII GAAGA 2 cut(s) 54, 227
MhlI GDGCHC 3 cut(s) 105, 113, 271
MluCI AATT 3 cut(s) 45, 468, 473
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 434
MnlI CCTC 7 cut(s) 133, 167, 175, 207, 254, 345, 444
MseI TTAA 1 cut(s) 478
MslI CAYNNNNRTG 1 cut(s) 84
MspA1I CMGCKG 2 cut(s) 226, 391
MspI CCGG 1 cut(s) 359
MwoI GCNNNNNNNGC 2 cut(s) 100, 308
NdeII GATC 1 cut(s) 431
NlaIII CATG 3 cut(s) 178, 250, 427
NlaIV GGNNCC 2 cut(s) 110, 408
PciSI GCTCTTC 1 cut(s) 210
PfeI GAWTC 1 cut(s) 142
PkrI GCNGC 2 cut(s) 222, 225
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
Psp124BI GAGCTC 1 cut(s) 105
PspFI CCCAGC 2 cut(s) 226, 391
PspN4I GGNNCC 2 cut(s) 110, 408
PstI CTGCAG 1 cut(s) 225
PvuII CAGCTG 2 cut(s) 226, 391
RseI CAYNNNNRTG 1 cut(s) 84
SacI GAGCTC 1 cut(s) 105
SalI GTCGAC 1 cut(s) 182
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 478
SatI GCNGC 2 cut(s) 221, 224
Sau3AI GATC 1 cut(s) 431
SchI GAGTC 1 cut(s) 179
SduI GDGCHC 3 cut(s) 105, 113, 271
SfaNI GCATC 2 cut(s) 23, 392
SfcI CTRYAG 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 3 cut(s) 45, 468, 473
SstI GAGCTC 1 cut(s) 105
TaaI ACNGT 2 cut(s) 67, 276
TaqI TCGA 2 cut(s) 183, 335
TasI AATT 3 cut(s) 45, 468, 473
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 1 cut(s) 478
Tru9I TTAA 1 cut(s) 478
TscAI CASTG 2 cut(s) 279, 319
TseI GCWGC 2 cut(s) 220, 223
TspDTI ATGAA 3 cut(s) 68, 163, 228
TspRI CASTG 2 cut(s) 279, 319
XapI RAATTY 1 cut(s) 473
XmiI GTMKAC 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.