RchiOBHm_Chr6g0294271

Inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015173
Physical Location & Seq
Forward (+)
56664155 .. 56664912
758 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
ATGAAGCCAATTACATCATTCTTCTTCCTCTCAACCCTGTGCGTGCTCCAACATGTTGCCCTCGTCGCAAATAGTCAACCCGGCAAACCCGTCGGAGGCCAACCGGGCAAATCCGTAGGAGGCCAACCAGGCGGAACCTCTGGGCCCGTCAGTGGTAATCAAATAGAAGAAGCATGCAAATACGCGACTAACAAGGATCTGTGCATCACCAACCTCAAGAAAGACCCAAACAGCCAAGGAGCTGACCTCGTCGGCCTGGCCTTGATTGCGATCAGGCTGGCCGCCGCGAATGCCGCTGACGTGGAATCCCACCTGAGAACGTTGATGATCAACAACGCCACCTCTCTAGCCCCGGAGGTTCAGCAGGGGGTTTCGGACTGTATAGAACATTACACCGACGCCAACGAGCAGCTTGACGACTCGGTGGCCGCGGTGTCGGCCAAGGAATTTCATGACGTCGAGATGTGGGTTAAGGTTGCCATTGGCGATGCGGCTTATTGTCAAAACGCCATGAAGGGTCAGCACACTGTGGTTGAACAGAAGAACAAATTGTTTACGCAGATGTGCGAAAATGTCTTGGCCGTGATCAAGGCCGTGACTCAAAAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

203

Amino Acids

21.47

Weight (kDa)

5.7

Isoelectric Point (pI)

28.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 54 - 195 2.6e-23 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55770 AT4G02250
fragaria_vesca FvH4_2g25950
malus_domestica MD03G1290800.v1.1 MD11G1307600.v1.1
rosa_chinensis RchiOBHm_Chr6g0294271
rosa_laevigata RLG00000011882
rosa_multiflora Rmu_co8483625.1_g000001 Rmu_sc0003996.1_g000007
rosa_roxburghii Rroxscaffold_7G00173490
rosa_rugosa Rorug06G0243400
rosa_samantha Rh6AG355600 Rh6BG362500 Rh6CG369600 Rh6DG356400
rosa_wichuraiana Rw6G030980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 459
AccII CGCG 3 cut(s) 185, 287, 431
AciI CCGC 7 cut(s) 132, 282, 285, 294, 429, 431, 491
AclI AACGTT 1 cut(s) 320
AclWI GGATC 1 cut(s) 204
AcoI YGGCCR 4 cut(s) 279, 426, 438, 579
AcsI RAATTY 1 cut(s) 446
AcyI GRCGYC 2 cut(s) 399, 456
AdeI CACNNNGTG 1 cut(s) 529
AfiI CCNNNNNNNGG 2 cut(s) 95, 152
AflIII ACRYGT 1 cut(s) 52
AgsI TTSAA 1 cut(s) 536
AjiI CACGTC 1 cut(s) 301
AjnI CCWGG 2 cut(s) 127, 255
AluBI AGCT 2 cut(s) 242, 412
AluI AGCT 2 cut(s) 242, 412
Alw21I GWGCWC 1 cut(s) 48
AlwI GGATC 1 cut(s) 204
ApaI GGGCCC 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 409
ApoI RAATTY 1 cut(s) 446
AspS9I GGNCC 2 cut(s) 143, 144
AsuC2I CCSGG 3 cut(s) 81, 105, 353
AsuHPI GGTGA 1 cut(s) 199
BaeGI GKGCMC 1 cut(s) 147
BanII GRGCYC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 1 cut(s) 421
BceAI ACGGC 2 cut(s) 566, 578
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 2 cut(s) 129, 257
BclI TGATCA 2 cut(s) 327, 585
BcnI CCSGG 3 cut(s) 81, 105, 353
BfaI CTAG 1 cut(s) 347
BglI GCCNNNNNGGC 2 cut(s) 105, 129
BisI GCNGC 6 cut(s) 282, 285, 294, 410, 429, 492
BlsI GCNGC 6 cut(s) 283, 286, 295, 411, 430, 493
Bme1390I CCNGG 5 cut(s) 81, 105, 129, 257, 353
BmgBI CACGTC 1 cut(s) 301
BmgT120I GGNCC 2 cut(s) 143, 144
BmiI GGNNCC 2 cut(s) 136, 145
BmrFI CCNGG 5 cut(s) 81, 105, 129, 257, 353
BmsI GCATC 2 cut(s) 213, 478
BplI GAGNNNNNCTC 2 cut(s) 231, 263
BpuEI CTTGAG 1 cut(s) 200
BpuMI CCSGG 3 cut(s) 81, 105, 353
BsaBI GATNNNNATC 1 cut(s) 269
BsaHI GRCGYC 2 cut(s) 399, 456
BsaJI CCNNGG 4 cut(s) 235, 351, 429, 441
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 152
Bse8I GATNNNNATC 1 cut(s) 269
BseBI CCWGG 2 cut(s) 129, 257
BseDI CCNNGG 4 cut(s) 235, 351, 429, 441
BseJI GATNNNNATC 1 cut(s) 269
BseLI CCNNNNNNNGG 2 cut(s) 95, 152
BseMII CTCAG 1 cut(s) 305
BseSI GKGCMC 1 cut(s) 147
BseXI GCAGC 1 cut(s) 421
Bsh1236I CGCG 3 cut(s) 185, 287, 431
BsiHKAI GWGCWC 1 cut(s) 48
BsiSI CCGG 3 cut(s) 81, 104, 353
BslI CCNNNNNNNGG 2 cut(s) 95, 152
BsmI GAATGC 1 cut(s) 295
Bsp120I GGGCCC 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 48, 147
Bsp143I GATC 4 cut(s) 196, 270, 327, 585
BspACI CCGC 7 cut(s) 132, 282, 285, 294, 429, 431, 491
BspCNI CTCAG 1 cut(s) 306
BspFNI CGCG 3 cut(s) 185, 287, 431
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 2 cut(s) 136, 145
BspPI GGATC 1 cut(s) 204
BssECI CCNNGG 4 cut(s) 235, 351, 429, 441
BssMI GATC 4 cut(s) 196, 270, 327, 585
BssNI GRCGYC 2 cut(s) 399, 456
BssT1I CCWWGG 2 cut(s) 235, 441
Bst2UI CCWGG 2 cut(s) 129, 257
Bst4CI ACNGT 2 cut(s) 380, 529
BstACI GRCGYC 2 cut(s) 399, 456
BstC8I GCNNGC 3 cut(s) 44, 175, 279
BstDEI CTNAG 1 cut(s) 314
BstDSI CCRYGG 1 cut(s) 429
BstFNI CGCG 3 cut(s) 185, 287, 431
BstKTI GATC 4 cut(s) 199, 273, 330, 588
BstMBI GATC 4 cut(s) 196, 270, 327, 585
BstMWI GCNNNNNNNGC 7 cut(s) 65, 105, 129, 266, 290, 293, 437
BstNI CCWGG 2 cut(s) 129, 257
BstNSI RCATGY 2 cut(s) 56, 177
BstSCI CCNGG 5 cut(s) 79, 103, 127, 255, 351
BstSLI GKGCMC 1 cut(s) 147
BstUI CGCG 3 cut(s) 185, 287, 431
BstV1I GCAGC 1 cut(s) 421
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BtgI CCRYGG 1 cut(s) 429
BtgZI GCGATG 1 cut(s) 501
BtrI CACGTC 1 cut(s) 301
BtsIMutI CAGTG 2 cut(s) 157, 525
Cac8I GCNNGC 3 cut(s) 44, 175, 279
CciI TCATGA 1 cut(s) 451
Cfr13I GGNCC 2 cut(s) 143, 144
Cfr42I CCGCGG 1 cut(s) 432
CseI GACGC 1 cut(s) 407
CviAII CATG 4 cut(s) 53, 174, 452, 511
DdeI CTNAG 1 cut(s) 314
DpnI GATC 4 cut(s) 198, 272, 329, 587
DpnII GATC 4 cut(s) 196, 270, 327, 585
DraIII CACNNNGTG 1 cut(s) 529
EaeI YGGCCR 4 cut(s) 279, 426, 438, 579
EciI GGCGGA 1 cut(s) 147
Eco130I CCWWGG 2 cut(s) 235, 441
Eco24I GRGCYC 1 cut(s) 147
EcoRII CCWGG 2 cut(s) 127, 255
EcoT14I CCWWGG 2 cut(s) 235, 441
EcoT38I GRGCYC 1 cut(s) 147
ErhI CCWWGG 2 cut(s) 235, 441
FaeI CATG 4 cut(s) 56, 177, 455, 514
FaiI YATR 5 cut(s) 54, 175, 383, 453, 512
FatI CATG 4 cut(s) 52, 173, 451, 510
FbaI TGATCA 2 cut(s) 327, 585
Fnu4HI GCNGC 6 cut(s) 282, 285, 294, 410, 429, 492
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 6 cut(s) 282, 285, 294, 410, 429, 492
FspBI CTAG 1 cut(s) 347
GluI GCNGC 6 cut(s) 282, 285, 294, 410, 429, 492
HapII CCGG 3 cut(s) 81, 104, 353
HgaI GACGC 1 cut(s) 407
Hin1I GRCGYC 2 cut(s) 399, 456
Hin1II CATG 4 cut(s) 56, 177, 455, 514
HincII GTYRAC 1 cut(s) 77
HindII GTYRAC 1 cut(s) 77
HinfI GANTC 3 cut(s) 305, 419, 598
HpaII CCGG 3 cut(s) 81, 104, 353
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 2 cut(s) 77, 555
Hpy188I TCNGA 2 cut(s) 95, 376
Hpy188III TCNNGA 3 cut(s) 217, 452, 460
Hpy8I GTNNAC 2 cut(s) 77, 555
Hpy99I CGWCG 5 cut(s) 68, 95, 254, 401, 461
HpyAV CCTTC 1 cut(s) 508
HpyCH4III ACNGT 2 cut(s) 380, 529
HpyCH4IV ACGT 3 cut(s) 300, 320, 456
HpyCH4V TGCA 2 cut(s) 177, 204
HpyF10VI GCNNNNNNNGC 7 cut(s) 65, 105, 129, 266, 290, 293, 437
HpyF3I CTNAG 1 cut(s) 314
HpySE526I ACGT 3 cut(s) 300, 320, 456
Hsp92I GRCGYC 2 cut(s) 399, 456
Hsp92II CATG 4 cut(s) 56, 177, 455, 514
Ksp22I TGATCA 2 cut(s) 327, 585
KspI CCGCGG 1 cut(s) 432
Kzo9I GATC 4 cut(s) 196, 270, 327, 585
LmnI GCTCC 2 cut(s) 51, 239
Lsp1109I GCAGC 1 cut(s) 421
LweI GCATC 2 cut(s) 213, 478
MaeI CTAG 1 cut(s) 347
MaeII ACGT 3 cut(s) 300, 320, 456
MaeIII GTNAC 1 cut(s) 595
MalI GATC 4 cut(s) 198, 272, 329, 587
MboI GATC 4 cut(s) 196, 270, 327, 585
MboII GAAGA 4 cut(s) 13, 16, 179, 553
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 2 cut(s) 48, 147
MluCI AATT 3 cut(s) 9, 446, 548
MlyI GAGTC 2 cut(s) 413, 592
MmeI TCCRAC 2 cut(s) 73, 73
MnlI CCTC 9 cut(s) 38, 71, 89, 113, 148, 224, 257, 349, 352
MseI TTAA 1 cut(s) 471
MspA1I CMGCKG 2 cut(s) 296, 431
MspI CCGG 3 cut(s) 81, 104, 353
MspR9I CCNGG 5 cut(s) 81, 105, 129, 257, 353
Mva1269I GAATGC 1 cut(s) 295
MvaI CCWGG 2 cut(s) 129, 257
MvnI CGCG 3 cut(s) 185, 287, 431
MwoI GCNNNNNNNGC 7 cut(s) 65, 105, 129, 266, 290, 293, 437
NciI CCSGG 3 cut(s) 81, 105, 353
NdeII GATC 4 cut(s) 196, 270, 327, 585
NlaIII CATG 4 cut(s) 56, 177, 455, 514
NlaIV GGNNCC 2 cut(s) 136, 145
NmuCI GTSAC 1 cut(s) 595
NspI RCATGY 2 cut(s) 56, 177
PaeI GCATGC 1 cut(s) 177
PagI TCATGA 1 cut(s) 451
PciI ACATGT 1 cut(s) 52
PctI GAATGC 1 cut(s) 295
PfeI GAWTC 1 cut(s) 305
PflFI GACNNNGTC 1 cut(s) 248
PkrI GCNGC 6 cut(s) 283, 286, 295, 411, 430, 493
PleI GAGTC 2 cut(s) 413, 592
PpsI GAGTC 2 cut(s) 413, 592
PscI ACATGT 1 cut(s) 52
Psp1406I AACGTT 1 cut(s) 320
Psp6I CCWGG 2 cut(s) 127, 255
PspGI CCWGG 2 cut(s) 127, 255
PspN4I GGNNCC 2 cut(s) 136, 145
PspOMI GGGCCC 1 cut(s) 143
PspPI GGNCC 2 cut(s) 143, 144
PsuI RGATCY 1 cut(s) 196
PsyI GACNNNGTC 1 cut(s) 248
SacII CCGCGG 1 cut(s) 432
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 6 cut(s) 282, 285, 294, 410, 429, 492
Sau3AI GATC 4 cut(s) 196, 270, 327, 585
Sau96I GGNCC 2 cut(s) 143, 144
SchI GAGTC 2 cut(s) 413, 592
ScrFI CCNGG 5 cut(s) 81, 105, 129, 257, 353
SduI GDGCHC 2 cut(s) 48, 147
SfaNI GCATC 2 cut(s) 213, 478
Sfr303I CCGCGG 1 cut(s) 432
SgrBI CCGCGG 1 cut(s) 432
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SphI GCATGC 1 cut(s) 177
Sse9I AATT 3 cut(s) 9, 446, 548
SsiI CCGC 7 cut(s) 132, 282, 285, 294, 429, 431, 491
SspMI CTAG 1 cut(s) 347
StyD4I CCNGG 5 cut(s) 79, 103, 127, 255, 351
StyI CCWWGG 2 cut(s) 235, 441
TaaI ACNGT 2 cut(s) 380, 529
TaiI ACGT 3 cut(s) 303, 323, 459
TaqI TCGA 1 cut(s) 459
TasI AATT 3 cut(s) 9, 446, 548
TauI GCSGC 5 cut(s) 284, 287, 296, 431, 494
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 2 cut(s) 157, 532
TseFI GTSAC 1 cut(s) 595
TseI GCWGC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 595
TspDTI ATGAA 3 cut(s) 17, 440, 527
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 2 cut(s) 157, 532
Tth111I GACNNNGTC 1 cut(s) 248
XapI RAATTY 1 cut(s) 446
XceI RCATGY 2 cut(s) 56, 177
XspI CTAG 1 cut(s) 347
ZraI GACGTC 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.