RchiOBHm_Chr5g0013681

C2 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016781
Physical Location & Seq
Forward (+)
9212845 .. 9213414
570 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
ATGAACCAAGTAGTTGAATTCCGGAGAAGGAGCAAGACGGCGGCGGCGGCCAAGCTCCGAGAGATAGAGGTCCTGATAATCTCGGCGCAAGACCTCAAGGACGTGAAGCACGTAACGCACATGAGACCTTACGCGGTGGTGTACGTGGAGAAGGACGTGCACGTGGCGAGGACGCGGGTGGACGAGCACGGCGGCACCAACCCCACGTGGAACGAGGTGGTGAGGGTGAAGTTCAGGGAGGAGATGGCGGACAAGGAGCACGTGCTGGCGGCGGTGAACGTGGACATCTACGCTCACGGGCACGTGAGGGAGAAGCCGGTGGGGAGAGCCAGGGTGCTGTTGTGCGACGTTTTGAAGGGCGGCGACGCCCGAGAGCCGGCGGACAATCCGATACAGTGCTTGACGGTGCAGGTGTGGAGGCCGTCCGGGAGGCCTCAGGGGCTGCTCAACCTGTGGGTCCCGCCCACCGGGAGGTTCTTGATAAGGAGGGAGTCGCTGTCGTTTAGTGAGAGGGAGGAGGCGGAGGAAGAGGAGGAGGTGGTGGCGGTGAAAGGTGATGAAGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

189

Amino Acids

21.55

Weight (kDa)

6.02

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 22 - 121 2.6e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016781)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05810
malus_domestica MD05G1286300.v1.1
prunus_persica Prupe.4G078600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0013681
rosa_laevigata RLG00000032040
rosa_multiflora Rmu_co8348725.1_g000001 Rmu_sc0002573.1_g000004
rosa_roxburghii Rroxscaffold_1G00062920
rosa_rugosa Rorug05G0012200
rosa_samantha Rh5AG106600 Rh5BG102700 Rh5CG114600 Rh5DG101400
rosa_wichuraiana Rw5G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 400
Acc36I ACCTGC 1 cut(s) 400
AccB1I GGYRCC 1 cut(s) 194
AccII CGCG 2 cut(s) 134, 175
AccIII TCCGGA 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 17
AcvI CACGTG 4 cut(s) 163, 207, 262, 304
AcyI GRCGYC 1 cut(s) 366
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 3 cut(s) 376, 467, 471
AgsI TTSAA 2 cut(s) 17, 355
AjiI CACGTC 2 cut(s) 103, 157
AjnI CCWGG 1 cut(s) 329
AluBI AGCT 1 cut(s) 55
AluI AGCT 1 cut(s) 55
Alw21I GWGCWC 3 cut(s) 162, 189, 261
Alw26I GTCTC 1 cut(s) 118
Alw44I GTGCAC 1 cut(s) 158
AlwNI CAGNNNCTG 1 cut(s) 442
Ama87I CYCGRG 1 cut(s) 369
Aor13HI TCCGGA 1 cut(s) 21
AoxI GGCC 3 cut(s) 48, 419, 431
ApaLI GTGCAC 1 cut(s) 158
ApeKI GCWGC 1 cut(s) 442
ApoI RAATTY 1 cut(s) 17
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 2 cut(s) 70, 457
AsuC2I CCSGG 2 cut(s) 427, 469
AsuHPI GGTGA 5 cut(s) 232, 238, 286, 559, 566
AvaI CYCGRG 1 cut(s) 369
AvaII GGWCC 2 cut(s) 70, 457
AxyI CCTNAGG 1 cut(s) 435
BaeGI GKGCMC 2 cut(s) 162, 303
BanI GGYRCC 1 cut(s) 194
BbrPI CACGTG 4 cut(s) 163, 207, 262, 304
Bbv12I GWGCWC 3 cut(s) 162, 189, 261
BbvI GCAGC 1 cut(s) 429
BccI CCATC 1 cut(s) 238
BceAI ACGGC 3 cut(s) 54, 205, 406
BciT130I CCWGG 1 cut(s) 331
BcnI CCSGG 2 cut(s) 427, 469
BcoDI GTCTC 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 400
BglI GCCNNNNNGGC 1 cut(s) 439
BisI GCNGC 7 cut(s) 42, 45, 48, 193, 270, 361, 443
BlsI GCNGC 7 cut(s) 43, 46, 49, 194, 271, 362, 444
Bme1390I CCNGG 3 cut(s) 331, 427, 469
Bme18I GGWCC 2 cut(s) 70, 457
BmeT110I CYCGRG 1 cut(s) 369
BmgBI CACGTC 2 cut(s) 103, 157
BmgT120I GGNCC 2 cut(s) 70, 457
BmiI GGNNCC 3 cut(s) 196, 458, 459
BmrFI CCNGG 3 cut(s) 331, 427, 469
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 2 cut(s) 427, 469
BsaAI YACGTR 6 cut(s) 112, 145, 163, 207, 262, 304
BsaHI GRCGYC 1 cut(s) 366
BsaI GGTCTC 1 cut(s) 118
BsaJI CCNNGG 1 cut(s) 330
BsaWI WCCGGW 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 3 cut(s) 376, 467, 471
Bse118I RCCGGY 2 cut(s) 316, 376
Bse21I CCTNAGG 1 cut(s) 435
BseAI TCCGGA 1 cut(s) 21
BseBI CCWGG 1 cut(s) 331
BseDI CCNNGG 1 cut(s) 330
BseLI CCNNNNNNNGG 3 cut(s) 376, 467, 471
BseMII CTCAG 1 cut(s) 449
BseRI GAGGAG 4 cut(s) 254, 530, 545, 548
BseSI GKGCMC 2 cut(s) 162, 303
BseXI GCAGC 1 cut(s) 429
BsgI GTGCAG 1 cut(s) 428
Bsh1236I CGCG 2 cut(s) 134, 175
BshFI GGCC 3 cut(s) 50, 421, 433
BshNI GGYRCC 1 cut(s) 194
BsiHKAI GWGCWC 3 cut(s) 162, 189, 261
BsiHKCI CYCGRG 1 cut(s) 369
BsiSI CCGG 5 cut(s) 22, 317, 377, 426, 468
BslFI GGGAC 1 cut(s) 443
BslI CCNNNNNNNGG 3 cut(s) 376, 467, 471
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 443
BsnI GGCC 3 cut(s) 50, 421, 433
Bso31I GGTCTC 1 cut(s) 118
BsoBI CYCGRG 1 cut(s) 369
Bsp1286I GDGCHC 4 cut(s) 162, 189, 261, 303
Bsp13I TCCGGA 1 cut(s) 21
BspANI GGCC 3 cut(s) 50, 421, 433
BspCNI CTCAG 1 cut(s) 448
BspEI TCCGGA 1 cut(s) 21
BspFNI CGCG 2 cut(s) 134, 175
BspLI GGNNCC 3 cut(s) 196, 458, 459
BspMI ACCTGC 1 cut(s) 400
BspT107I GGYRCC 1 cut(s) 194
BspTNI GGTCTC 1 cut(s) 118
BsrFI RCCGGY 2 cut(s) 316, 376
BssAI RCCGGY 2 cut(s) 316, 376
BssECI CCNNGG 1 cut(s) 330
BssNI GRCGYC 1 cut(s) 366
Bst2UI CCWGG 1 cut(s) 331
Bst4CI ACNGT 2 cut(s) 396, 406
Bst6I CTCTTC 2 cut(s) 522, 555
BstACI GRCGYC 1 cut(s) 366
BstBAI YACGTR 6 cut(s) 112, 145, 163, 207, 262, 304
BstC8I GCNNGC 2 cut(s) 267, 378
BstDEI CTNAG 1 cut(s) 435
BstFNI CGCG 2 cut(s) 134, 175
BstHHI GCGC 1 cut(s) 88
BstMAI GTCTC 1 cut(s) 118
BstMWI GCNNNNNNNGC 3 cut(s) 47, 115, 439
BstNI CCWGG 1 cut(s) 331
BstSCI CCNGG 3 cut(s) 329, 425, 467
BstSLI GKGCMC 2 cut(s) 162, 303
BstUI CGCG 2 cut(s) 134, 175
BstV1I GCAGC 1 cut(s) 429
Bsu36I CCTNAGG 1 cut(s) 435
BsuRI GGCC 3 cut(s) 50, 421, 433
BtrI CACGTC 2 cut(s) 103, 157
BtsIMutI CAGTG 1 cut(s) 401
BveI ACCTGC 1 cut(s) 400
Cac8I GCNNGC 2 cut(s) 267, 378
CaiI CAGNNNCTG 1 cut(s) 442
CfoI GCGC 1 cut(s) 88
Cfr10I RCCGGY 2 cut(s) 316, 376
Cfr13I GGNCC 2 cut(s) 70, 457
CseI GACGC 2 cut(s) 181, 374
Csp6I GTAC 1 cut(s) 142
CviAII CATG 1 cut(s) 121
CviJI RGCY 8 cut(s) 50, 55, 316, 329, 376, 421, 433, 442
CviKI_1 RGCY 8 cut(s) 50, 55, 316, 329, 376, 421, 433, 442
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 1 cut(s) 435
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 2 cut(s) 522, 555
EarI CTCTTC 2 cut(s) 522, 555
EciI GGCGGA 3 cut(s) 263, 395, 536
Eco147I AGGCCT 1 cut(s) 433
Eco31I GGTCTC 1 cut(s) 118
Eco47I GGWCC 2 cut(s) 70, 457
Eco72I CACGTG 4 cut(s) 163, 207, 262, 304
Eco81I CCTNAGG 1 cut(s) 435
Eco88I CYCGRG 1 cut(s) 369
EcoO109I RGGNCCY 2 cut(s) 70, 457
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 329
FaeI CATG 1 cut(s) 124
FaiI YATR 1 cut(s) 122
FaqI GGGAC 1 cut(s) 443
FatI CATG 1 cut(s) 120
FauI CCCGC 2 cut(s) 168, 468
Fnu4HI GCNGC 7 cut(s) 42, 45, 48, 193, 270, 361, 443
Fsp4HI GCNGC 7 cut(s) 42, 45, 48, 193, 270, 361, 443
GlaI GCGC 1 cut(s) 87
GluI GCNGC 7 cut(s) 42, 45, 48, 193, 270, 361, 443
HaeIII GGCC 3 cut(s) 50, 421, 433
HapII CCGG 5 cut(s) 22, 317, 377, 426, 468
HgaI GACGC 2 cut(s) 181, 374
HhaI GCGC 1 cut(s) 88
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 1 cut(s) 124
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HinfI GANTC 1 cut(s) 491
HpaII CCGG 5 cut(s) 22, 317, 377, 426, 468
HphI GGTGA 5 cut(s) 232, 238, 286, 559, 566
Hpy166II GTNNAC 5 cut(s) 142, 160, 181, 277, 283
Hpy188I TCNGA 2 cut(s) 59, 390
Hpy188III TCNNGA 3 cut(s) 22, 73, 478
Hpy8I GTNNAC 5 cut(s) 142, 160, 181, 277, 283
Hpy99I CGWCG 2 cut(s) 350, 368
HpyAV CCTTC 3 cut(s) 21, 145, 349
HpyCH4III ACNGT 2 cut(s) 396, 406
HpyCH4V TGCA 2 cut(s) 160, 409
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 115, 439
HpyF3I CTNAG 1 cut(s) 435
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 1 cut(s) 124
HspAI GCGC 1 cut(s) 86
KflI GGGWCCC 1 cut(s) 457
Kpn2I TCCGGA 1 cut(s) 21
KroI GCCGGC 1 cut(s) 376
KroNI GCCGGC 1 cut(s) 378
LmnI GCTCC 3 cut(s) 30, 60, 256
Lsp1109I GCAGC 1 cut(s) 429
MaeIII GTNAC 1 cut(s) 112
MboII GAAGA 1 cut(s) 539
MhlI GDGCHC 4 cut(s) 162, 189, 261, 303
MluCI AATT 1 cut(s) 17
MlyI GAGTC 1 cut(s) 500
MroI TCCGGA 1 cut(s) 21
MroNI GCCGGC 1 cut(s) 376
MspI CCGG 5 cut(s) 22, 317, 377, 426, 468
MspR9I CCNGG 3 cut(s) 331, 427, 469
MvaI CCWGG 1 cut(s) 331
MvnI CGCG 2 cut(s) 134, 175
MwoI GCNNNNNNNGC 3 cut(s) 47, 115, 439
NaeI GCCGGC 1 cut(s) 378
NciI CCSGG 2 cut(s) 427, 469
NgoMIV GCCGGC 1 cut(s) 376
NlaIII CATG 1 cut(s) 124
NlaIV GGNNCC 3 cut(s) 196, 458, 459
NmeAIII GCCGAG 1 cut(s) 62
PaqCI CACCTGC 1 cut(s) 400
PceI AGGCCT 1 cut(s) 433
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdiI GCCGGC 1 cut(s) 378
PfoI TCCNGGA 1 cut(s) 425
PkrI GCNGC 7 cut(s) 43, 46, 49, 194, 271, 362, 444
PleI GAGTC 1 cut(s) 499
PmaCI CACGTG 4 cut(s) 163, 207, 262, 304
PmlI CACGTG 4 cut(s) 163, 207, 262, 304
PpsI GAGTC 1 cut(s) 499
Ppu21I YACGTR 6 cut(s) 112, 145, 163, 207, 262, 304
PpuMI RGGWCCY 2 cut(s) 70, 457
Psp5II RGGWCCY 2 cut(s) 70, 457
Psp6I CCWGG 1 cut(s) 329
PspCI CACGTG 4 cut(s) 163, 207, 262, 304
PspGI CCWGG 1 cut(s) 329
PspN4I GGNNCC 3 cut(s) 196, 458, 459
PspPI GGNCC 2 cut(s) 70, 457
PspPPI RGGWCCY 2 cut(s) 70, 457
PstNI CAGNNNCTG 1 cut(s) 442
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SatI GCNGC 7 cut(s) 42, 45, 48, 193, 270, 361, 443
Sau96I GGNCC 2 cut(s) 70, 457
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 3 cut(s) 331, 427, 469
SduI GDGCHC 4 cut(s) 162, 189, 261, 303
SinI GGWCC 2 cut(s) 70, 457
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 1 cut(s) 17
SseBI AGGCCT 1 cut(s) 433
StuI AGGCCT 1 cut(s) 433
StyD4I CCNGG 3 cut(s) 329, 425, 467
TaaI ACNGT 2 cut(s) 396, 406
TasI AATT 1 cut(s) 17
TauI GCSGC 6 cut(s) 44, 47, 50, 195, 272, 363
TscAI CASTG 1 cut(s) 401
TseI GCWGC 1 cut(s) 442
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 401
VneI GTGCAC 1 cut(s) 158
VpaK11BI GGWCC 2 cut(s) 70, 457
XapI RAATTY 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.