RchiOBHm_Chr5g0024561

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016515
Physical Location & Seq
Forward (+)
18668308 .. 18670235
1928 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1515 bp
ATGGCAATGGAGAAGGTACTTGCTTCTGTGGGAGAGATGAGGCCAAAGACCAAGATTGTGTGCACACTGGGACCATCTTCCCGGTCAGTGGAGATGGCAGAGAAGCTTTTGAGGGCTGGCATGAACGTTGCTCGCTTCAACTTCTCTCACGGTAGTCATGAATATCATCAAGAAACTCTTGATCATCTAAGGACTGCCATGAACAACACGGGAATTCTCTGTGCTATCATGTTGGATACGAAGGGTCCAGAAATTAGAACTGGGTTTCTGAAAGATGAGAAGGCCATTCATCTTCAACAAGGAAAAGAGATTACTATATCAACTGACTATACCATTCATGGTGATGAGAATATGATCTCTATGAGCTACAAGAAGTTGGCTGAGGATGTGAAGCCTCAAAGTGATATCTTGTGTGCTGATGGAACAATTACTTTGAAGGTTCTGGCATGTGACAAGGAGCGTGGTTTGGTACGTTGTCGATGTGAGAACACTGCAATTTTGGGTGAGAGGAAGAATGTCAATCTTCCTGGCGTTGTTGTTGATCTGCCAACTTTGACAGAGAAGGACAAAGTGGATATCTTGGAGTGGGGAGTTCCAAATAAGATTGACATGATTGCGCTCTCTTTTGTTAGGAAAGGCTCAGACCTTGTTGAGGTCAGAAATGTTTTGGGGGAGCATGCCAAGGATATAATACTCATGTCCAAGGTTGAGAATCAAGAAGGTGTTACCAATTTCGACGAAATACTAGCAAAATCAGATGCCTTCATGGTGGCGAGGGGTGACCTTGGAATGGAAATTCCAATTGAAAAGATATTCCTAGCTCAGAAACTGATGATTGCAAAGGCAAACAGACTAGGAAAGCCAGTGGTGACTGCTACTCAGATGCTAGAGTCCATGACCAGGTCTCCTCGTCCCACTCGAGCAGAAGCCACTGATGTTGCCAATGCTGTTCTTGATGGAACTGACTGTGTCATGCTCAGTGGCGAAACTGCAGCTGGAGACTATCCGGAAATAGCCGTTCAAACAATGGCCAAAATCTGTGTTTCTGCAGAACAGTCCATAAACTATAACGAACTTTTCAAGAAAATGGAAACCGAAGCAATGCCTATGAGCCCTCAAGAGAGTTTAGCCTCCTCGGCGGTGCACATGGCAACTTCCATCAGGGCAGCACTCATTTTGGTCCTGACCAGAGGAGGAACCACAGCAAAGCTTGTGGCTAAGTACAGGCCAAGCATGCCGATTTTGTCTGTGGTGGTTCCTCAGCTGACTACAGATTCTTTTGAGTGGTCATGCAGCGATGCAGCTCCTGCAAGGCACAGCCTTATTTATAGGGGTCTTGTGCCTCTTCTCAGCTCAGGGGTCATTAGAGCTTCTCAGGCTGAGTCCACTGAAGAGACTGTGCAGTCTGCGATTCAACATGCAAAGGCTAAAGGACTTTGCAAGCCTGGGGATTCAATCGTGGCTCTGCACCGAATGAATTGCATCGACGTGTGCAATGTTTTCCCTGTCATGTGA

Protein Analysis

504

Amino Acids

55.01

Weight (kDa)

6.17

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 16 - 340 1.4e-146 Pyruvate kinase, barrel domain
PK_C PF02887 374 - 492 7.6e-23 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04050 AT3G25960 AT3G55650
fragaria_vesca FvH4_3g14970
malus_domestica MD10G1210700.v1.1
prunus_persica Prupe.4G134000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0024561
rosa_laevigata RLG00000032807
rosa_multiflora Rmu_sc0005699.1_g000007
rosa_rugosa Rorug05G0084000
rosa_samantha Rh5AG175700 Rh5CG189100 Rh5DG174800
rosa_wichuraiana Rw5G015880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1402
AccIII TCCGGA 1 cut(s) 1006
AciI CCGC 1 cut(s) 1139
AclI AACGTT 1 cut(s) 126
AcoI YGGCCR 1 cut(s) 1029
AcsI RAATTY 2 cut(s) 213, 795
AcuI CTGAAG 1 cut(s) 1410
AfaI GTAC 3 cut(s) 18, 471, 1223
AfiI CCNNNNNNNGG 4 cut(s) 88, 652, 790, 900
AflIII ACRYGT 1 cut(s) 1488
AgsI TTSAA 8 cut(s) 139, 296, 436, 806, 1022, 1081, 1415, 1455
AjiI CACGTC 1 cut(s) 1489
AjnI CCWGG 3 cut(s) 526, 899, 1444
AluBI AGCT 9 cut(s) 106, 366, 821, 995, 1210, 1264, 1304, 1353, 1370
AluI AGCT 9 cut(s) 106, 366, 821, 995, 1210, 1264, 1304, 1353, 1370
Alw21I GWGCWC 2 cut(s) 65, 1146
Alw26I GTCTC 3 cut(s) 909, 993, 1388
Alw44I GTGCAC 2 cut(s) 61, 1142
AlwNI CAGNNNCTG 2 cut(s) 829, 1307
Ama87I CYCGRG 1 cut(s) 918
Aor13HI TCCGGA 1 cut(s) 1006
AoxI GGCC 4 cut(s) 41, 282, 1029, 1226
ApaLI GTGCAC 2 cut(s) 61, 1142
ApeKI GCWGC 4 cut(s) 992, 1166, 1293, 1301
ApoI RAATTY 2 cut(s) 213, 795
AspLEI GCGC 1 cut(s) 619
AspS9I GGNCC 3 cut(s) 71, 245, 1180
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 4 cut(s) 353, 515, 791, 880
AvaI CYCGRG 1 cut(s) 918
AvaII GGWCC 3 cut(s) 71, 245, 1180
BaeGI GKGCMC 2 cut(s) 65, 1146
BalI TGGCCA 1 cut(s) 1031
BanII GRGCYC 1 cut(s) 1115
Bbv12I GWGCWC 2 cut(s) 65, 1146
BbvCI CCTCAGC 2 cut(s) 381, 1260
BbvI GCAGC 4 cut(s) 1004, 1178, 1305, 1313
BccI CCATC 5 cut(s) 82, 88, 413, 950, 1166
BceAI ACGGC 1 cut(s) 1001
BcgI CGANNNNNNTGC 2 cut(s) 1461, 1495
BciT130I CCWGG 3 cut(s) 528, 901, 1446
BciVI GTATCC 1 cut(s) 229
BclI TGATCA 1 cut(s) 181
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 3 cut(s) 909, 993, 1388
BfaI CTAG 4 cut(s) 746, 818, 854, 887
BfmI CTRYAG 3 cut(s) 990, 1047, 1269
BfuI GTATCC 1 cut(s) 229
BglI GCCNNNNNGGC 1 cut(s) 1136
BisI GCNGC 4 cut(s) 993, 1167, 1294, 1302
BlsI GCNGC 4 cut(s) 994, 1168, 1295, 1303
Bme1390I CCNGG 4 cut(s) 82, 528, 901, 1446
Bme18I GGWCC 3 cut(s) 71, 245, 1180
BmeT110I CYCGRG 1 cut(s) 918
BmgBI CACGTC 1 cut(s) 1489
BmgT120I GGNCC 3 cut(s) 71, 245, 1180
BmiI GGNNCC 4 cut(s) 72, 246, 1198, 1257
BmrFI CCNGG 4 cut(s) 82, 528, 901, 1446
BmrI ACTGGG 2 cut(s) 77, 270
BmsI GCATC 4 cut(s) 748, 873, 1288, 1491
BmuI ACTGGG 2 cut(s) 77, 270
BpmI CTGGAG 1 cut(s) 1017
Bpu10I CCTNAGC 3 cut(s) 381, 1260, 1354
BpuEI CTTGAG 1 cut(s) 1101
BpuMI CCSGG 1 cut(s) 82
BsaI GGTCTC 1 cut(s) 909
BsaJI CCNNGG 5 cut(s) 681, 702, 784, 1134, 1445
BsaWI WCCGGW 1 cut(s) 1006
BsaXI ACNNNNNCTCC 2 cut(s) 889, 919
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 652, 790, 900
Bse1I ACTGG 3 cut(s) 72, 265, 863
Bse3DI GCAATG 3 cut(s) 12, 1107, 1501
BseAI TCCGGA 1 cut(s) 1006
BseBI CCWGG 3 cut(s) 528, 901, 1446
BseDI CCNNGG 5 cut(s) 681, 702, 784, 1134, 1445
BseGI GGATG 1 cut(s) 391
BseLI CCNNNNNNNGG 4 cut(s) 88, 652, 790, 900
BseMI GCAATG 3 cut(s) 12, 1107, 1501
BseNI ACTGG 3 cut(s) 72, 265, 863
BseRI GAGGAG 3 cut(s) 897, 1123, 1206
BseSI GKGCMC 2 cut(s) 65, 1146
BseXI GCAGC 4 cut(s) 1004, 1178, 1305, 1313
BsgI GTGCAG 2 cut(s) 1421, 1451
BshFI GGCC 4 cut(s) 43, 284, 1031, 1228
BsiHKAI GWGCWC 2 cut(s) 65, 1146
BsiHKCI CYCGRG 1 cut(s) 918
BsiSI CCGG 2 cut(s) 82, 1007
BslFI GGGAC 2 cut(s) 84, 897
BslI CCNNNNNNNGG 4 cut(s) 88, 652, 790, 900
BsmAI GTCTC 3 cut(s) 909, 993, 1388
BsmFI GGGAC 2 cut(s) 84, 897
BsnI GGCC 4 cut(s) 43, 284, 1031, 1228
Bso31I GGTCTC 1 cut(s) 909
BsoBI CYCGRG 1 cut(s) 918
Bsp1286I GDGCHC 3 cut(s) 65, 1115, 1146
Bsp13I TCCGGA 1 cut(s) 1006
Bsp143I GATC 3 cut(s) 181, 354, 541
BspACI CCGC 1 cut(s) 1139
BspANI GGCC 4 cut(s) 43, 284, 1031, 1228
BspEI TCCGGA 1 cut(s) 1006
BspHI TCATGA 1 cut(s) 157
BspLI GGNNCC 4 cut(s) 72, 246, 1198, 1257
BspMAI CTGCAG 2 cut(s) 994, 1051
BspTNI GGTCTC 1 cut(s) 909
BsrDI GCAATG 3 cut(s) 12, 1107, 1501
BsrI ACTGG 3 cut(s) 72, 265, 863
BssECI CCNNGG 5 cut(s) 681, 702, 784, 1134, 1445
BssMI GATC 3 cut(s) 181, 354, 541
BssT1I CCWWGG 3 cut(s) 681, 702, 784
Bst2UI CCWGG 3 cut(s) 528, 901, 1446
Bst4CI ACNGT 4 cut(s) 152, 968, 1056, 1399
Bst6I CTCTTC 2 cut(s) 1350, 1386
BstAPI GCANNNNNTGC 1 cut(s) 1307
BstC8I GCNNGC 5 cut(s) 118, 133, 678, 1235, 1442
BstEII GGTNACC 1 cut(s) 779
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 1 cut(s) 391
BstHHI GCGC 1 cut(s) 619
BstKTI GATC 3 cut(s) 184, 357, 544
BstMAI GTCTC 3 cut(s) 909, 993, 1388
BstMBI GATC 3 cut(s) 181, 354, 541
BstMWI GCNNNNNNNGC 4 cut(s) 1136, 1234, 1307, 1376
BstNI CCWGG 3 cut(s) 528, 901, 1446
BstNSI RCATGY 4 cut(s) 450, 680, 1237, 1421
BstPI GGTNACC 1 cut(s) 779
BstSCI CCNGG 4 cut(s) 80, 526, 899, 1444
BstSFI CTRYAG 3 cut(s) 990, 1047, 1269
BstSLI GKGCMC 2 cut(s) 65, 1146
BstV1I GCAGC 4 cut(s) 1004, 1178, 1305, 1313
BsuI GTATCC 1 cut(s) 229
BsuRI GGCC 4 cut(s) 43, 284, 1031, 1228
BtgZI GCGATG 1 cut(s) 1311
BtrI CACGTC 1 cut(s) 1489
BtsCI GGATG 1 cut(s) 391
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 7 cut(s) 65, 93, 489, 870, 930, 985, 1386
Cac8I GCNNGC 5 cut(s) 118, 133, 678, 1235, 1442
CaiI CAGNNNCTG 2 cut(s) 829, 1307
CciI TCATGA 1 cut(s) 157
CfoI GCGC 1 cut(s) 619
Cfr13I GGNCC 3 cut(s) 71, 245, 1180
CsiI ACCWGGT 1 cut(s) 899
Csp6I GTAC 3 cut(s) 17, 470, 1222
CspCI CAANNNNNGTGG 6 cut(s) 442, 477, 919, 954, 1194, 1229
CviQI GTAC 3 cut(s) 17, 470, 1222
DpnI GATC 3 cut(s) 183, 356, 543
DpnII GATC 3 cut(s) 181, 354, 541
DrdI GACNNNNNNGTC 1 cut(s) 1402
DseDI GACNNNNNNGTC 1 cut(s) 1402
EaeI YGGCCR 1 cut(s) 1029
Eam1104I CTCTTC 2 cut(s) 1350, 1386
EarI CTCTTC 2 cut(s) 1350, 1386
Eco130I CCWWGG 3 cut(s) 681, 702, 784
Eco24I GRGCYC 1 cut(s) 1115
Eco31I GGTCTC 1 cut(s) 909
Eco32I GATATC 2 cut(s) 406, 577
Eco47I GGWCC 3 cut(s) 71, 245, 1180
Eco57I CTGAAG 1 cut(s) 1410
Eco88I CYCGRG 1 cut(s) 918
Eco91I GGTNACC 1 cut(s) 779
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoO65I GGTNACC 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 213
EcoRII CCWGG 3 cut(s) 526, 899, 1444
EcoRV GATATC 2 cut(s) 406, 577
EcoT14I CCWWGG 3 cut(s) 681, 702, 784
EcoT38I GRGCYC 1 cut(s) 1115
ErhI CCWWGG 3 cut(s) 681, 702, 784
FalI AAGNNNNNCTT 2 cut(s) 162, 194
FaqI GGGAC 2 cut(s) 84, 897
FbaI TGATCA 1 cut(s) 181
Fnu4HI GCNGC 4 cut(s) 993, 1167, 1294, 1302
FokI GGATG 1 cut(s) 398
FriOI GRGCYC 1 cut(s) 1115
Fsp4HI GCNGC 4 cut(s) 993, 1167, 1294, 1302
FspBI CTAG 4 cut(s) 746, 818, 854, 887
GlaI GCGC 1 cut(s) 618
GluI GCNGC 4 cut(s) 993, 1167, 1294, 1302
GsuI CTGGAG 1 cut(s) 1017
HaeIII GGCC 4 cut(s) 43, 284, 1031, 1228
HapII CCGG 2 cut(s) 82, 1007
HhaI GCGC 1 cut(s) 619
Hin6I GCGC 1 cut(s) 617
HinP1I GCGC 1 cut(s) 617
HindIII AAGCTT 2 cut(s) 104, 1208
HinfI GANTC 6 cut(s) 712, 890, 1274, 1382, 1411, 1451
HpaII CCGG 2 cut(s) 82, 1007
HphI GGTGA 4 cut(s) 353, 515, 791, 880
Hpy166II GTNNAC 3 cut(s) 63, 1144, 1386
Hpy188I TCNGA 6 cut(s) 270, 643, 659, 757, 825, 882
Hpy8I GTNNAC 3 cut(s) 63, 1144, 1386
Hpy99I CGWCG 2 cut(s) 740, 1490
HpyAV CCTTC 7 cut(s) 7, 235, 274, 430, 556, 713, 772
HpyCH4III ACNGT 4 cut(s) 152, 968, 1056, 1399
HpyCH4IV ACGT 3 cut(s) 126, 472, 1488
HpyF10VI GCNNNNNNNGC 4 cut(s) 1136, 1234, 1307, 1376
HpySE526I ACGT 3 cut(s) 126, 472, 1488
HspAI GCGC 1 cut(s) 617
Kpn2I TCCGGA 1 cut(s) 1006
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 3 cut(s) 181, 354, 541
LmnI GCTCC 3 cut(s) 457, 673, 1309
Lsp1109I GCAGC 4 cut(s) 1004, 1178, 1305, 1313
LweI GCATC 4 cut(s) 748, 873, 1288, 1491
MabI ACCWGGT 1 cut(s) 899
MaeI CTAG 4 cut(s) 746, 818, 854, 887
MaeII ACGT 3 cut(s) 126, 472, 1488
MaeIII GTNAC 4 cut(s) 449, 724, 779, 868
MalI GATC 3 cut(s) 183, 356, 543
MboI GATC 3 cut(s) 181, 354, 541
MboII GAAGA 6 cut(s) 69, 284, 515, 523, 1337, 1403
MfeI CAATTG 1 cut(s) 801
MhlI GDGCHC 3 cut(s) 65, 1115, 1146
MlsI TGGCCA 1 cut(s) 1031
MluCI AATT 8 cut(s) 213, 252, 426, 495, 730, 795, 801, 1477
MluNI TGGCCA 1 cut(s) 1031
MlyI GAGTC 2 cut(s) 899, 1391
MmeI TCCRAC 1 cut(s) 213
Mox20I TGGCCA 1 cut(s) 1031
MroI TCCGGA 1 cut(s) 1006
MscI TGGCCA 1 cut(s) 1031
MslI CAYNNNNRTG 2 cut(s) 342, 1487
Msp20I TGGCCA 1 cut(s) 1031
MspA1I CMGCKG 2 cut(s) 995, 1264
MspI CCGG 2 cut(s) 82, 1007
MspR9I CCNGG 4 cut(s) 82, 528, 901, 1446
MunI CAATTG 1 cut(s) 801
MvaI CCWGG 3 cut(s) 528, 901, 1446
MwoI GCNNNNNNNGC 4 cut(s) 1136, 1234, 1307, 1376
NciI CCSGG 1 cut(s) 82
NdeII GATC 3 cut(s) 181, 354, 541
NlaIV GGNNCC 4 cut(s) 72, 246, 1198, 1257
NmeAIII GCCGAG 1 cut(s) 1115
NmuCI GTSAC 3 cut(s) 449, 779, 868
NspI RCATGY 4 cut(s) 450, 680, 1237, 1421
PaeI GCATGC 2 cut(s) 680, 1237
PaeR7I CTCGAG 1 cut(s) 918
PagI TCATGA 1 cut(s) 157
PcsI WCGNNNNNNNCGW 1 cut(s) 916
PfeI GAWTC 4 cut(s) 712, 1274, 1411, 1451
PflFI GACNNNGTC 2 cut(s) 901, 968
PkrI GCNGC 4 cut(s) 994, 1168, 1295, 1303
PleI GAGTC 2 cut(s) 898, 1390
PpsI GAGTC 2 cut(s) 898, 1390
Psp1406I AACGTT 1 cut(s) 126
Psp6I CCWGG 3 cut(s) 526, 899, 1444
PspEI GGTNACC 1 cut(s) 779
PspGI CCWGG 3 cut(s) 526, 899, 1444
PspN4I GGNNCC 4 cut(s) 72, 246, 1198, 1257
PspPI GGNCC 3 cut(s) 71, 245, 1180
PspXI VCTCGAGB 1 cut(s) 918
PstI CTGCAG 2 cut(s) 994, 1051
PstNI CAGNNNCTG 2 cut(s) 829, 1307
PsyI GACNNNGTC 2 cut(s) 901, 968
PvuII CAGCTG 2 cut(s) 995, 1264
RsaI GTAC 3 cut(s) 18, 471, 1223
RsaNI GTAC 3 cut(s) 17, 470, 1222
RseI CAYNNNNRTG 2 cut(s) 342, 1487
SatI GCNGC 4 cut(s) 993, 1167, 1294, 1302
Sau3AI GATC 3 cut(s) 181, 354, 541
Sau96I GGNCC 3 cut(s) 71, 245, 1180
SchI GAGTC 2 cut(s) 899, 1391
ScrFI CCNGG 4 cut(s) 82, 528, 901, 1446
SduI GDGCHC 3 cut(s) 65, 1115, 1146
SexAI ACCWGGT 1 cut(s) 899
SfaNI GCATC 4 cut(s) 748, 873, 1288, 1491
SfcI CTRYAG 3 cut(s) 990, 1047, 1269
Sfr274I CTCGAG 1 cut(s) 918
SinI GGWCC 3 cut(s) 71, 245, 1180
SlaI CTCGAG 1 cut(s) 918
SmiMI CAYNNNNRTG 2 cut(s) 342, 1487
SmlI CTYRAG 2 cut(s) 918, 1116
SmoI CTYRAG 2 cut(s) 918, 1116
SphI GCATGC 2 cut(s) 680, 1237
Sse9I AATT 8 cut(s) 213, 252, 426, 495, 730, 795, 801, 1477
SsiI CCGC 1 cut(s) 1139
SspMI CTAG 4 cut(s) 746, 818, 854, 887
StyD4I CCNGG 4 cut(s) 80, 526, 899, 1444
StyI CCWWGG 3 cut(s) 681, 702, 784
TaaI ACNGT 4 cut(s) 152, 968, 1056, 1399
TaiI ACGT 3 cut(s) 129, 475, 1491
TaqI TCGA 4 cut(s) 478, 735, 919, 1485
TasI AATT 8 cut(s) 213, 252, 426, 495, 730, 795, 801, 1477
TatI WGTACW 1 cut(s) 1221
TfiI GAWTC 4 cut(s) 712, 1274, 1411, 1451
TscAI CASTG 7 cut(s) 72, 93, 496, 870, 937, 985, 1393
TseFI GTSAC 3 cut(s) 449, 779, 868
TseI GCWGC 4 cut(s) 992, 1166, 1293, 1301
Tsp45I GTSAC 3 cut(s) 449, 779, 868
TspDTI ATGAA 7 cut(s) 137, 174, 215, 278, 326, 754, 1490
TspRI CASTG 7 cut(s) 72, 93, 496, 870, 937, 985, 1393
Tth111I GACNNNGTC 2 cut(s) 901, 968
VneI GTGCAC 2 cut(s) 61, 1142
VpaK11BI GGWCC 3 cut(s) 71, 245, 1180
XagI CCTNNNNNAGG 1 cut(s) 650
XapI RAATTY 2 cut(s) 213, 795
XceI RCATGY 4 cut(s) 450, 680, 1237, 1421
XhoI CTCGAG 1 cut(s) 918
XspI CTAG 4 cut(s) 746, 818, 854, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.