RchiOBHm_Chr4g0447321

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0019002
Physical Location & Seq
Reverse (-)
67127665 .. 67133060
5396 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1167 bp
ATGTGCAGAAATTTAAATCTGAGCCCGTGTACAAGAATTCTGGTTAATGGTCGCATCTGCTTCCTGTTACAGTTCGTGACCGAAAGCTTATGGTCCCCTTTAGGGACTTATTTGGCAAATGTTAAGAAACAGGGTATCATTCTTTTTAGAGGCACCTCTGCCTTACATCATGTGGCCACCTGTCTGCATCCCAAGGTTTTTGAGGTGAAATGGGTTGAATTTTCGCCTGGTGAGAATTATTTGGTGTCTACAGTGTTGAACAAAAAAACACTGAGAGCTGTAATAAGCATTTTTGATGTGAAAACTGGAAAAGTAGTGAAAAAAATTGTGGGAGGTCCTCATGATTTTGCAAATAAAGGAGTTGGGGCGTCTTGGCCCATTTTCAAATGGTCCGGTGGAAGAGATGACAAGTATTTTGCCCTAATCAGCAAGAATGTTATCTCTATTTATGAGATAAATACATTTACAGTCATATTGACGGTAGGGAATGTCACGGACTTCAGTTGGTCACCAACTGATGCGATTCTTGCTCTCTTTGTATCAGAATCGGTTGATGCTGGTGGTGCCATACCTGCTTTGGTGAAACTTGTTCAAATACCCAATAAAGAGCAGTTGATTGAGAAGAAGCTTTCTAGTGTTAGTAACTGCAAGATGTACTGGCAAAGCAATGGGGAGTATCTTGCTATGAAAGTTGACAGCAGTACAAACAATTTCGTTGAGCTTTTCCAAATTAAAGATCGAGGCATCGATAATAAAATTTTGGTGCTCCCGTATAATGAGGACAAGGTCACTGCTTTTGCTTGGGAACCCAAGGGCCATAGGTTTGCTATTATTCAGAGTAATGAAGATATTAGTTTCTACTCCATGCGGGATGCTGCCAGCACAGGCTGTGTTTCATTGCTCACGATTCTCAGAAGCAAGGGGACAAACTATCTCTTTTGGTCACCTTCTGGTGGCCTCATAATACTTGCAAGGTTGAACGGTTCCAATGGGAATTTGGAATTTTTCAATGTGGATGAACTTCGGACAGTTGCTATATCTAAACATCAACGGCTCTCGAGTGGAACCCAACCGAGATATGTTGCTACTTCTACTCCTGGGACTTGTCATGAGAAGCCGAGTTTTAACGTTTGGTCTTTTGATGGCCACCTACTCTACCAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

388

Amino Acids

43.22

Weight (kDa)

9.16

Isoelectric Point (pI)

29.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF2A PF08662 208 - 387 9.9e-24 Eukaryotic translation initiation factor eIF2A
Beta-prop_WDR19_1st PF23389 218 - 351 7.6e-07 WDR19 first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019002)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0447321
rosa_laevigata RLG00000026012
rosa_multiflora Rmu_ssc0000396.1_g000008
rosa_roxburghii Rroxscaffold_5G00387700
rosa_samantha Rh4AG441000 Rh4BG450300 Rh4CG467400 Rh4DG448700
rosa_wichuraiana Rw4G037750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 2 cut(s) 152, 563
AccI GTMKAC 1 cut(s) 248
AciI CCGC 1 cut(s) 868
AclI AACGTT 1 cut(s) 1128
AcoI YGGCCR 2 cut(s) 174, 1144
AcsI RAATTY 6 cut(s) 10, 36, 218, 756, 994, 1001
AcuI CTGAAG 1 cut(s) 484
AcyI GRCGYC 1 cut(s) 368
AfaI GTAC 3 cut(s) 31, 656, 703
AfiI CCNNNNNNNGG 2 cut(s) 102, 953
AgsI TTSAA 6 cut(s) 218, 259, 385, 593, 979, 1009
AjnI CCWGG 2 cut(s) 226, 1096
AluBI AGCT 4 cut(s) 87, 278, 628, 721
AluI AGCT 4 cut(s) 87, 278, 628, 721
Alw21I GWGCWC 1 cut(s) 768
Ama87I CYCGRG 1 cut(s) 1057
AoxI GGCC 5 cut(s) 174, 374, 814, 955, 1144
ApeKI GCWGC 1 cut(s) 875
ApoI RAATTY 6 cut(s) 10, 36, 218, 756, 994, 1001
ArsI GACNNNNNNTTYG 2 cut(s) 398, 430
AspS9I GGNCC 5 cut(s) 93, 335, 375, 390, 814
AsuHPI GGTGA 5 cut(s) 217, 242, 501, 592, 936
AvaI CYCGRG 1 cut(s) 1057
AvaII GGWCC 3 cut(s) 93, 335, 390
BalI TGGCCA 2 cut(s) 176, 1146
BanI GGYRCC 2 cut(s) 152, 563
BanII GRGCYC 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 768
BbvI GCAGC 1 cut(s) 862
BccI CCATC 1 cut(s) 1136
BceAI ACGGC 1 cut(s) 1067
BciT130I CCWGG 2 cut(s) 228, 1098
BfaI CTAG 1 cut(s) 633
BfmI CTRYAG 1 cut(s) 249
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 1 cut(s) 876
BlsI GCNGC 1 cut(s) 877
Bme1390I CCNGG 2 cut(s) 228, 1098
Bme18I GGWCC 3 cut(s) 93, 335, 390
BmeT110I CYCGRG 1 cut(s) 1057
BmgT120I GGNCC 5 cut(s) 93, 335, 375, 390, 814
BmiI GGNNCC 6 cut(s) 95, 154, 565, 807, 985, 1066
BmrFI CCNGG 2 cut(s) 228, 1098
BmsI GCATC 6 cut(s) 63, 196, 508, 544, 753, 862
Bsa29I ATCGAT 1 cut(s) 747
BsaHI GRCGYC 1 cut(s) 368
BsaJI CCNNGG 3 cut(s) 192, 810, 1097
BsaWI WCCGGW 1 cut(s) 392
BsaXI ACNNNNNCTCC 2 cut(s) 1078, 1108
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 953
Bse1I ACTGG 2 cut(s) 310, 662
Bse3DI GCAATG 2 cut(s) 673, 896
BseBI CCWGG 2 cut(s) 228, 1098
BseCI ATCGAT 1 cut(s) 747
BseDI CCNNGG 3 cut(s) 192, 810, 1097
BseGI GGATG 3 cut(s) 187, 877, 1021
BseLI CCNNNNNNNGG 2 cut(s) 102, 953
BseMI GCAATG 2 cut(s) 673, 896
BseMII CTCAG 3 cut(s) 11, 263, 925
BseNI ACTGG 2 cut(s) 310, 662
BseXI GCAGC 1 cut(s) 862
BsgI GTGCAG 1 cut(s) 25
BshFI GGCC 5 cut(s) 176, 376, 816, 957, 1146
BshNI GGYRCC 2 cut(s) 152, 563
BshVI ATCGAT 1 cut(s) 747
BsiHKAI GWGCWC 1 cut(s) 768
BsiHKCI CYCGRG 1 cut(s) 1057
BsiSI CCGG 1 cut(s) 393
BslFI GGGAC 4 cut(s) 79, 118, 937, 1114
BslI CCNNNNNNNGG 2 cut(s) 102, 953
BsmFI GGGAC 4 cut(s) 79, 118, 937, 1114
BsnI GGCC 5 cut(s) 176, 376, 816, 957, 1146
BsoBI CYCGRG 1 cut(s) 1057
Bsp1286I GDGCHC 2 cut(s) 26, 768
Bsp1407I TGTACA 1 cut(s) 29
Bsp143I GATC 1 cut(s) 736
BspACI CCGC 1 cut(s) 868
BspANI GGCC 5 cut(s) 176, 376, 816, 957, 1146
BspCNI CTCAG 3 cut(s) 12, 264, 924
BspDI ATCGAT 1 cut(s) 747
BspHI TCATGA 2 cut(s) 340, 1108
BspLI GGNNCC 6 cut(s) 95, 154, 565, 807, 985, 1066
BspMI ACCTGC 1 cut(s) 580
BspT107I GGYRCC 2 cut(s) 152, 563
BsrDI GCAATG 2 cut(s) 673, 896
BsrGI TGTACA 1 cut(s) 29
BsrI ACTGG 2 cut(s) 310, 662
BssECI CCNNGG 3 cut(s) 192, 810, 1097
BssMI GATC 1 cut(s) 736
BssNI GRCGYC 1 cut(s) 368
BssT1I CCWWGG 2 cut(s) 192, 810
Bst2UI CCWGG 2 cut(s) 228, 1098
Bst4CI ACNGT 6 cut(s) 72, 253, 469, 481, 983, 1030
Bst6I CTCTTC 1 cut(s) 394
BstACI GRCGYC 1 cut(s) 368
BstAUI TGTACA 1 cut(s) 29
BstC8I GCNNGC 1 cut(s) 880
BstDEI CTNAG 3 cut(s) 20, 272, 911
BstEII GGTNACC 2 cut(s) 507, 942
BstF5I GGATG 3 cut(s) 187, 877, 1021
BstKTI GATC 1 cut(s) 739
BstMBI GATC 1 cut(s) 736
BstMWI GCNNNNNNNGC 3 cut(s) 527, 563, 572
BstNI CCWGG 2 cut(s) 228, 1098
BstPI GGTNACC 2 cut(s) 507, 942
BstSCI CCNGG 2 cut(s) 226, 1096
BstSFI CTRYAG 1 cut(s) 249
BstV1I GCAGC 1 cut(s) 862
Bsu15I ATCGAT 1 cut(s) 747
BsuRI GGCC 5 cut(s) 176, 376, 816, 957, 1146
BsuTUI ATCGAT 1 cut(s) 747
BtsCI GGATG 3 cut(s) 187, 877, 1021
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 3 cut(s) 258, 269, 789
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 1 cut(s) 880
CciI TCATGA 2 cut(s) 340, 1108
Cfr13I GGNCC 5 cut(s) 93, 335, 375, 390, 814
ClaI ATCGAT 1 cut(s) 747
CseI GACGC 1 cut(s) 357
Csp6I GTAC 3 cut(s) 30, 655, 702
CviAII CATG 4 cut(s) 170, 341, 865, 1109
CviQI GTAC 3 cut(s) 30, 655, 702
DdeI CTNAG 3 cut(s) 20, 272, 911
DpnI GATC 1 cut(s) 738
DpnII GATC 1 cut(s) 736
DraI TTTAAA 1 cut(s) 15
EaeI YGGCCR 2 cut(s) 174, 1144
Eam1104I CTCTTC 1 cut(s) 394
EarI CTCTTC 1 cut(s) 394
Eco130I CCWWGG 2 cut(s) 192, 810
Eco24I GRGCYC 1 cut(s) 26
Eco47I GGWCC 3 cut(s) 93, 335, 390
Eco57I CTGAAG 1 cut(s) 484
Eco88I CYCGRG 1 cut(s) 1057
Eco91I GGTNACC 2 cut(s) 507, 942
EcoO109I RGGNCCY 1 cut(s) 335
EcoO65I GGTNACC 2 cut(s) 507, 942
EcoRI GAATTC 1 cut(s) 36
EcoRII CCWGG 2 cut(s) 226, 1096
EcoT14I CCWWGG 2 cut(s) 192, 810
EcoT38I GRGCYC 1 cut(s) 26
ErhI CCWWGG 2 cut(s) 192, 810
FaeI CATG 4 cut(s) 173, 344, 868, 1112
FaqI GGGAC 4 cut(s) 79, 118, 937, 1114
FatI CATG 4 cut(s) 169, 340, 864, 1108
FauI CCCGC 1 cut(s) 861
FblI GTMKAC 1 cut(s) 248
Fnu4HI GCNGC 1 cut(s) 876
FokI GGATG 3 cut(s) 174, 884, 1028
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 1 cut(s) 876
FspBI CTAG 1 cut(s) 633
GluI GCNGC 1 cut(s) 876
HaeIII GGCC 5 cut(s) 176, 376, 816, 957, 1146
HapII CCGG 1 cut(s) 393
HgaI GACGC 1 cut(s) 357
Hin1I GRCGYC 1 cut(s) 368
Hin1II CATG 4 cut(s) 173, 344, 868, 1112
HincII GTYRAC 1 cut(s) 694
HindII GTYRAC 1 cut(s) 694
HindIII AAGCTT 2 cut(s) 85, 626
HinfI GANTC 3 cut(s) 523, 545, 907
HpaII CCGG 1 cut(s) 393
HphI GGTGA 5 cut(s) 217, 242, 501, 592, 936
Hpy166II GTNNAC 3 cut(s) 30, 249, 694
Hpy188I TCNGA 5 cut(s) 21, 544, 837, 914, 1026
Hpy188III TCNNGA 5 cut(s) 76, 341, 904, 1057, 1109
Hpy8I GTNNAC 3 cut(s) 30, 249, 694
HpyAV CCTTC 1 cut(s) 957
HpyCH4III ACNGT 6 cut(s) 72, 253, 469, 481, 983, 1030
HpyCH4IV ACGT 1 cut(s) 1128
HpyCH4V TGCA 5 cut(s) 6, 187, 350, 648, 971
HpyF10VI GCNNNNNNNGC 3 cut(s) 527, 563, 572
HpyF3I CTNAG 3 cut(s) 20, 272, 911
HpySE526I ACGT 1 cut(s) 1128
Hsp92I GRCGYC 1 cut(s) 368
Hsp92II CATG 4 cut(s) 173, 344, 868, 1112
Kzo9I GATC 1 cut(s) 736
LmnI GCTCC 1 cut(s) 771
Lsp1109I GCAGC 1 cut(s) 862
LweI GCATC 6 cut(s) 63, 196, 508, 544, 753, 862
MaeI CTAG 1 cut(s) 633
MaeII ACGT 1 cut(s) 1128
MaeIII GTNAC 7 cut(s) 66, 76, 490, 507, 641, 787, 942
MalI GATC 1 cut(s) 738
MboI GATC 1 cut(s) 736
MboII GAAGA 3 cut(s) 411, 634, 857
MhlI GDGCHC 2 cut(s) 26, 768
MlsI TGGCCA 2 cut(s) 176, 1146
MluNI TGGCCA 2 cut(s) 176, 1146
MnlI CCTC 8 cut(s) 143, 166, 196, 326, 348, 734, 772, 968
Mox20I TGGCCA 2 cut(s) 176, 1146
MscI TGGCCA 2 cut(s) 176, 1146
MseI TTAA 5 cut(s) 14, 45, 123, 732, 1125
Msp20I TGGCCA 2 cut(s) 176, 1146
MspI CCGG 1 cut(s) 393
MspR9I CCNGG 2 cut(s) 228, 1098
MvaI CCWGG 2 cut(s) 228, 1098
MwoI GCNNNNNNNGC 3 cut(s) 527, 563, 572
NdeII GATC 1 cut(s) 736
NlaIII CATG 4 cut(s) 173, 344, 868, 1112
NlaIV GGNNCC 6 cut(s) 95, 154, 565, 807, 985, 1066
NmeAIII GCCGAG 1 cut(s) 1143
NmuCI GTSAC 5 cut(s) 76, 490, 507, 787, 942
PaeR7I CTCGAG 1 cut(s) 1057
PagI TCATGA 2 cut(s) 340, 1108
PfeI GAWTC 3 cut(s) 523, 545, 907
PflFI GACNNNGTC 1 cut(s) 785
PkrI GCNGC 1 cut(s) 877
PpuMI RGGWCCY 1 cut(s) 335
Psp1406I AACGTT 1 cut(s) 1128
Psp5II RGGWCCY 1 cut(s) 335
Psp6I CCWGG 2 cut(s) 226, 1096
PspEI GGTNACC 2 cut(s) 507, 942
PspGI CCWGG 2 cut(s) 226, 1096
PspN4I GGNNCC 6 cut(s) 95, 154, 565, 807, 985, 1066
PspPI GGNCC 5 cut(s) 93, 335, 375, 390, 814
PspPPI RGGWCCY 1 cut(s) 335
PsyI GACNNNGTC 1 cut(s) 785
RsaI GTAC 3 cut(s) 31, 656, 703
RsaNI GTAC 3 cut(s) 30, 655, 702
SaqAI TTAA 5 cut(s) 14, 45, 123, 732, 1125
SatI GCNGC 1 cut(s) 876
Sau3AI GATC 1 cut(s) 736
Sau96I GGNCC 5 cut(s) 93, 335, 375, 390, 814
ScrFI CCNGG 2 cut(s) 228, 1098
SduI GDGCHC 2 cut(s) 26, 768
SfaNI GCATC 6 cut(s) 63, 196, 508, 544, 753, 862
SfcI CTRYAG 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 1057
SinI GGWCC 3 cut(s) 93, 335, 390
SlaI CTCGAG 1 cut(s) 1057
SmiI ATTTAAAT 1 cut(s) 15
SmlI CTYRAG 1 cut(s) 1057
SmoI CTYRAG 1 cut(s) 1057
SsiI CCGC 1 cut(s) 868
SspMI CTAG 1 cut(s) 633
StyD4I CCNGG 2 cut(s) 226, 1096
StyI CCWWGG 2 cut(s) 192, 810
SwaI ATTTAAAT 1 cut(s) 15
TaaI ACNGT 6 cut(s) 72, 253, 469, 481, 983, 1030
TaiI ACGT 1 cut(s) 1131
TaqI TCGA 3 cut(s) 739, 747, 1058
TaqII GACCGA 1 cut(s) 95
TatI WGTACW 3 cut(s) 29, 654, 701
TfiI GAWTC 3 cut(s) 523, 545, 907
Tru1I TTAA 5 cut(s) 14, 45, 123, 732, 1125
Tru9I TTAA 5 cut(s) 14, 45, 123, 732, 1125
TscAI CASTG 3 cut(s) 258, 276, 796
TseFI GTSAC 5 cut(s) 76, 490, 507, 787, 942
TseI GCWGC 1 cut(s) 875
Tsp45I GTSAC 5 cut(s) 76, 490, 507, 787, 942
TspDTI ATGAA 4 cut(s) 701, 858, 885, 1032
TspGWI ACGGA 1 cut(s) 509
TspRI CASTG 3 cut(s) 258, 276, 796
Tth111I GACNNNGTC 1 cut(s) 785
VpaK11BI GGWCC 3 cut(s) 93, 335, 390
XapI RAATTY 6 cut(s) 10, 36, 218, 756, 994, 1001
XcmI CCANNNNNNNNNTGG 2 cut(s) 574, 994
XhoI CTCGAG 1 cut(s) 1057
XmiI GTMKAC 1 cut(s) 248
XspI CTAG 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.