Rh4DG448700

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0019002
Physical Location & Seq
Reverse (-)
64774319 .. 64778928
4610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG448700.1

Sequence Viewer

Length: 867 bp
ATGTGGCCACCTGTCTGCATCCCAAGTGTTGAACAAAAAAACACTGGTGGAATAAGCAGAGCTGTAATAAGCATTTTTGATGTGAAAACTGGAAAAGTAGTGAAAAAAATTGTGGGAGGTCCTCATGATTTTGCAAATAAAGGAGTTGGGGCGTCTTGGCCCATTTTCAAATGGTCCGGTGGAAGAGATGACAAGTATTTTGCCCTAATCAGCAAGAATGTTATCTCTATTTATGAGATAAATACATTTACAGTCATATTGACGGTAGGGAATGTCACGGACTTCAGTTGGTCACCAACTGATGCGATTCTTGCTCTCTTTGTATCAGAATCGGTTGATGCTGGTGGTGCCATACCTGCTTTGGTGAAACTTGTTCAAATACCCAATAAAGAGCAGTTGATTGAGAAGAAGCTTTCTAGTGTTAGTAACTGCAAGATGTACTGGCAAAGCAATGGGGAGTATCTTGCTATGAAAGTTGACAGCAGTACAAACAATTTCGTTGAGCTTTTCCAAATTAAAGATCGAGGCATCGATAATAAAATTTTGGTGCTCCCGTATAATGAGGACAAGGTCACTGCTTTTGCTTGGGAACCCAAGGGCCATAGGTTTGCTATTATTCAGAGTAATGAAGATATTAGTTTCTACTCCATGCGGGATGCTGCCAGCACAGGCTGTGTTTCATTGCTCACGATTCTCAGAAGCAAGGGGACAAACTATCTCTTTTGGTCACCTTCTGGTGGCCTCATAATACTTGCAAGGTTGAACGGTTCCAATGGGAATTTGGAATTTTTCAATGTGGATGAACTTCGGACAGTTGCTATATCTAAACATGTAGAAAATTCGTCTAATCATGGTTATCATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

288

Amino Acids

31.78

Weight (kDa)

8.3

Isoelectric Point (pI)

26.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF2A PF08662 136 - 280 4.6e-21 Eukaryotic translation initiation factor eIF2A
Beta-prop_WDR19_1st PF23389 146 - 277 9.1e-07 WDR19 first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019002)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0447321
rosa_laevigata RLG00000026012
rosa_multiflora Rmu_ssc0000396.1_g000008
rosa_roxburghii Rroxscaffold_5G00387700
rosa_samantha Rh4AG441000 Rh4BG450300 Rh4CG467400 Rh4DG448700
rosa_wichuraiana Rw4G037750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 364
AccB1I GGYRCC 1 cut(s) 347
AciI CCGC 1 cut(s) 652
AcoI YGGCCR 1 cut(s) 5
AcsI RAATTY 4 cut(s) 540, 778, 785, 838
AcuI CTGAAG 1 cut(s) 268
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 2 cut(s) 440, 487
AfiI CCNNNNNNNGG 1 cut(s) 737
AflIII ACRYGT 1 cut(s) 829
AgsI TTSAA 5 cut(s) 32, 169, 377, 763, 793
AluBI AGCT 3 cut(s) 62, 412, 505
AluI AGCT 3 cut(s) 62, 412, 505
Alw21I GWGCWC 1 cut(s) 552
AoxI GGCC 4 cut(s) 5, 158, 598, 739
ApeKI GCWGC 1 cut(s) 659
ApoI RAATTY 4 cut(s) 540, 778, 785, 838
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
AspS9I GGNCC 4 cut(s) 119, 159, 174, 598
AsuHPI GGTGA 3 cut(s) 285, 376, 720
AvaII GGWCC 2 cut(s) 119, 174
BalI TGGCCA 1 cut(s) 7
BanI GGYRCC 1 cut(s) 347
Bbv12I GWGCWC 1 cut(s) 552
BbvI GCAGC 1 cut(s) 646
BfaI CTAG 1 cut(s) 417
BfuAI ACCTGC 1 cut(s) 364
BisI GCNGC 1 cut(s) 660
BlsI GCNGC 1 cut(s) 661
Bme18I GGWCC 2 cut(s) 119, 174
BmgT120I GGNCC 4 cut(s) 119, 159, 174, 598
BmiI GGNNCC 3 cut(s) 349, 591, 769
BmsI GCATC 5 cut(s) 27, 292, 328, 537, 646
Bsa29I ATCGAT 1 cut(s) 531
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 594
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 1 cut(s) 737
Bse1I ACTGG 3 cut(s) 49, 94, 446
Bse3DI GCAATG 2 cut(s) 457, 680
BseCI ATCGAT 1 cut(s) 531
BseDI CCNNGG 1 cut(s) 594
BseGI GGATG 3 cut(s) 18, 661, 805
BseLI CCNNNNNNNGG 1 cut(s) 737
BseMI GCAATG 2 cut(s) 457, 680
BseMII CTCAG 1 cut(s) 709
BseNI ACTGG 3 cut(s) 49, 94, 446
BseXI GCAGC 1 cut(s) 646
BshFI GGCC 4 cut(s) 7, 160, 600, 741
BshNI GGYRCC 1 cut(s) 347
BshVI ATCGAT 1 cut(s) 531
BsiHKAI GWGCWC 1 cut(s) 552
BsiSI CCGG 1 cut(s) 177
BslFI GGGAC 1 cut(s) 721
BslI CCNNNNNNNGG 1 cut(s) 737
BsmFI GGGAC 1 cut(s) 721
BsnI GGCC 4 cut(s) 7, 160, 600, 741
Bsp1286I GDGCHC 1 cut(s) 552
Bsp143I GATC 1 cut(s) 520
BspACI CCGC 1 cut(s) 652
BspANI GGCC 4 cut(s) 7, 160, 600, 741
BspCNI CTCAG 1 cut(s) 708
BspDI ATCGAT 1 cut(s) 531
BspHI TCATGA 1 cut(s) 124
BspLI GGNNCC 3 cut(s) 349, 591, 769
BspMI ACCTGC 1 cut(s) 364
BspT107I GGYRCC 1 cut(s) 347
BsrDI GCAATG 2 cut(s) 457, 680
BsrI ACTGG 3 cut(s) 49, 94, 446
BssECI CCNNGG 1 cut(s) 594
BssMI GATC 1 cut(s) 520
BssNI GRCGYC 1 cut(s) 152
BssT1I CCWWGG 1 cut(s) 594
Bst4CI ACNGT 4 cut(s) 253, 265, 767, 814
Bst6I CTCTTC 1 cut(s) 178
BstACI GRCGYC 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 664
BstDEI CTNAG 2 cut(s) 695, 864
BstEII GGTNACC 2 cut(s) 291, 726
BstF5I GGATG 3 cut(s) 18, 661, 805
BstKTI GATC 1 cut(s) 523
BstMBI GATC 1 cut(s) 520
BstMWI GCNNNNNNNGC 3 cut(s) 311, 347, 356
BstNSI RCATGY 1 cut(s) 833
BstPI GGTNACC 2 cut(s) 291, 726
BstV1I GCAGC 1 cut(s) 646
Bsu15I ATCGAT 1 cut(s) 531
BsuRI GGCC 4 cut(s) 7, 160, 600, 741
BsuTUI ATCGAT 1 cut(s) 531
BtsCI GGATG 3 cut(s) 18, 661, 805
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 2 cut(s) 42, 573
BveI ACCTGC 1 cut(s) 364
Cac8I GCNNGC 1 cut(s) 664
CciI TCATGA 1 cut(s) 124
Cfr13I GGNCC 4 cut(s) 119, 159, 174, 598
ClaI ATCGAT 1 cut(s) 531
CseI GACGC 1 cut(s) 141
Csp6I GTAC 2 cut(s) 439, 486
CviAII CATG 4 cut(s) 125, 649, 830, 851
CviJI RGCY 8 cut(s) 7, 62, 160, 412, 505, 600, 672, 741
CviKI_1 RGCY 8 cut(s) 7, 62, 160, 412, 505, 600, 672, 741
CviQI GTAC 2 cut(s) 439, 486
DdeI CTNAG 2 cut(s) 695, 864
DpnI GATC 1 cut(s) 522
DpnII GATC 1 cut(s) 520
EaeI YGGCCR 1 cut(s) 5
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 594
Eco47I GGWCC 2 cut(s) 119, 174
Eco57I CTGAAG 1 cut(s) 268
Eco91I GGTNACC 2 cut(s) 291, 726
EcoO109I RGGNCCY 1 cut(s) 119
EcoO65I GGTNACC 2 cut(s) 291, 726
EcoT14I CCWWGG 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 4 cut(s) 128, 652, 833, 854
FaqI GGGAC 1 cut(s) 721
FatI CATG 4 cut(s) 124, 648, 829, 850
FauI CCCGC 1 cut(s) 645
Fnu4HI GCNGC 1 cut(s) 660
FokI GGATG 3 cut(s) 5, 668, 812
Fsp4HI GCNGC 1 cut(s) 660
FspBI CTAG 1 cut(s) 417
GluI GCNGC 1 cut(s) 660
HaeIII GGCC 4 cut(s) 7, 160, 600, 741
HapII CCGG 1 cut(s) 177
HgaI GACGC 1 cut(s) 141
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 4 cut(s) 128, 652, 833, 854
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 3 cut(s) 307, 329, 691
HpaII CCGG 1 cut(s) 177
HphI GGTGA 3 cut(s) 285, 376, 720
Hpy166II GTNNAC 1 cut(s) 478
Hpy188I TCNGA 4 cut(s) 328, 621, 698, 810
Hpy188III TCNNGA 2 cut(s) 125, 688
Hpy8I GTNNAC 1 cut(s) 478
HpyAV CCTTC 1 cut(s) 741
HpyCH4III ACNGT 4 cut(s) 253, 265, 767, 814
HpyCH4V TGCA 4 cut(s) 18, 134, 432, 755
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 347, 356
HpyF3I CTNAG 2 cut(s) 695, 864
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 4 cut(s) 128, 652, 833, 854
Kzo9I GATC 1 cut(s) 520
LmnI GCTCC 1 cut(s) 555
Lsp1109I GCAGC 1 cut(s) 646
LweI GCATC 5 cut(s) 27, 292, 328, 537, 646
MaeI CTAG 1 cut(s) 417
MaeIII GTNAC 5 cut(s) 274, 291, 425, 571, 726
MalI GATC 1 cut(s) 522
MboI GATC 1 cut(s) 520
MboII GAAGA 3 cut(s) 195, 418, 641
MhlI GDGCHC 1 cut(s) 552
MlsI TGGCCA 1 cut(s) 7
MluCI AATT 7 cut(s) 108, 493, 513, 540, 778, 785, 838
MluNI TGGCCA 1 cut(s) 7
MnlI CCTC 5 cut(s) 110, 132, 518, 556, 752
Mox20I TGGCCA 1 cut(s) 7
MscI TGGCCA 1 cut(s) 7
MseI TTAA 1 cut(s) 516
Msp20I TGGCCA 1 cut(s) 7
MspI CCGG 1 cut(s) 177
MwoI GCNNNNNNNGC 3 cut(s) 311, 347, 356
NdeII GATC 1 cut(s) 520
NlaIII CATG 4 cut(s) 128, 652, 833, 854
NlaIV GGNNCC 3 cut(s) 349, 591, 769
NmuCI GTSAC 4 cut(s) 274, 291, 571, 726
NspI RCATGY 1 cut(s) 833
PagI TCATGA 1 cut(s) 124
PciI ACATGT 1 cut(s) 829
PfeI GAWTC 3 cut(s) 307, 329, 691
PflFI GACNNNGTC 1 cut(s) 569
PkrI GCNGC 1 cut(s) 661
PpuMI RGGWCCY 1 cut(s) 119
PscI ACATGT 1 cut(s) 829
Psp5II RGGWCCY 1 cut(s) 119
PspEI GGTNACC 2 cut(s) 291, 726
PspN4I GGNNCC 3 cut(s) 349, 591, 769
PspPI GGNCC 4 cut(s) 119, 159, 174, 598
PspPPI RGGWCCY 1 cut(s) 119
PsyI GACNNNGTC 1 cut(s) 569
RsaI GTAC 2 cut(s) 440, 487
RsaNI GTAC 2 cut(s) 439, 486
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 1 cut(s) 660
Sau3AI GATC 1 cut(s) 520
Sau96I GGNCC 4 cut(s) 119, 159, 174, 598
SduI GDGCHC 1 cut(s) 552
SfaNI GCATC 5 cut(s) 27, 292, 328, 537, 646
SinI GGWCC 2 cut(s) 119, 174
Sse9I AATT 7 cut(s) 108, 493, 513, 540, 778, 785, 838
SsiI CCGC 1 cut(s) 652
SspMI CTAG 1 cut(s) 417
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 4 cut(s) 253, 265, 767, 814
TaqI TCGA 2 cut(s) 523, 531
TasI AATT 7 cut(s) 108, 493, 513, 540, 778, 785, 838
TatI WGTACW 2 cut(s) 438, 485
TfiI GAWTC 3 cut(s) 307, 329, 691
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 2 cut(s) 49, 580
TseFI GTSAC 4 cut(s) 274, 291, 571, 726
TseI GCWGC 1 cut(s) 659
Tsp45I GTSAC 4 cut(s) 274, 291, 571, 726
TspDTI ATGAA 4 cut(s) 485, 642, 669, 816
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 2 cut(s) 49, 580
Tth111I GACNNNGTC 1 cut(s) 569
VpaK11BI GGWCC 2 cut(s) 119, 174
XapI RAATTY 4 cut(s) 540, 778, 785, 838
XceI RCATGY 1 cut(s) 833
XcmI CCANNNNNNNNNTGG 2 cut(s) 358, 778
XspI CTAG 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.