Rroxscaffold_5G00387700

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0019002
Physical Location & Seq
Reverse (-)
66299460 .. 66306946
7487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00387700.1

Sequence Viewer

Length: 2331 bp
ATGTTTCCGATGCCTAACCCAATTGATGACATCATCATCCTCCCGCCACATGACCATCCATGCTTATCATTATGGGACAGGCTTCTGAACTTGAACTTGAACTTCATTGTCCAAAACATAAACAAGATCTTCTTCACAAGTAATATTGTACCAGAAATCCTTTCCTCTGTCTCGTCTCTCTCATTTTGTAACATCCAGAAGTCCACTCCTCTCTGTCTCGTCTTTCTGATTTTGTCTCTGTCAATTGTAGCGAGAAACATTTTTCAGGACTCGGAAATGGTGAAGGCACAAGGAGCGAGCGATCACCTCGTGAAAATTTTCCCTGAAAATATGCAGGACATTCCACAGGATGAGAACTCCAAAGATCAGTTTCTAATTCAATCCAAGTCAGGGGGTACCCAGGTTTTATGGAATAATGTGCAGAAATTGAAATCTGAGCCCGTGTACAAGAATTCTTTCGTGACCGAAAGCTTATGGTCCCCTTTAGGGACTTATTTGGCAAATGTTAAGAAGCAGGATATCATTCTTTTTAGAGGCACCTCTGCCTTACATCATGTGGCCACCTGTCTGCATCCCAAGGTTTTTGAGGTGAAATGGGTTGATTTTTCGCCTGGTGAGAATTATTTGGTGTCCTACAGTGCTGAACAAAAAAACAGTGGTGAAATAAGCAGAGCTGCAATAAGCATTTTTGATGTGAAAACTGGAAAAGTTGTGAAAAAAATTGTGGGAGGTCCTCATGATTTTGCAAATAAAGGAGTTGGGGCGTCTTGGCCCAGTTTCAAATGGTCCGGTGGAAGAGATGACAAGTATTTTGCCCTAATCAGCAAGAATGTTATCTCTATTTATGAGATAAATACATTTACAGCTGTATTGACGGTAGGGAATGTCATGGACTTCAGTTGGTCACCAACTGATGCGATTCTTGCTCTCTTTGTATCAGAATCGGTTGATGCTGGTGGTGCCATACCTGCTTTGGTGAAACTTGTTCAAATACCCAGTAAAGAGCAGTTGATTGAGAAGAAGCTTTCTAGTGTTAGGAAGTGCAAGATGTACTGGCAAAGCAATGGGGAGTATCTTGCTATGAAAGTTGACAACAGTACAAACAATTTCGTTGAGCTTTTCCAAATTAAAGATCGAGGCATCGATAATAAAATTTTGGAGCTACCGTATAATAAGGACAAGGTCACTGCTTTTGCTTGGGAACCCAAGGGCCATAGGTTTGCTATTATTCAGAGTAATGAAGATATTAGTTTCTACTCCATGCGGGATGCTGCCAGCACAGGCTGTGTTTCATTGCTCACGATTCTCAGAAGCAAGGGGACAAACTATCTCTTTTGGTCACCTTCAGGTGGCCTCATAATACTTGCAAGGTTGAATGGTTCCAATGGGAATTTGGAATTTTTCAATGTGGATGAGCTTCGGACAGTTGCTATATCTAAACATATAGCAACGGCTCTCGAGTGGAACCCAACCGGGAGATATGTTGCTACTTCTACTCCTGGGACTTGTCATGAGAAGCCGAGTTTTAACATTTGGTCTTTTGATGGCCGCCTACTCTACCAATTACCGAAGGATCTCTTCACAAAGTTTTCATGGCGACCAAGGCCACTCAACATGGCGACTAAGGCTATTATGGGGTCCATGAGCCATAACAGCTTGCATTCACTGAACATGGAAGATGATGAGTATTCACTCAACATGGAAGATGATGAGTATTCACTCAACATGGAAGATGATGATAATTCCATGAGTTTGACTGGGGGTATCAAGAAGTCTGTGGCTTTCTTGGAAAAGTTTCACGAAGATTTGGATGCCTTTTATAGGCTGGATGATGAGGAAAGGCAGTTATTGATAGACCTCAAGTACCTCACTTTGAGCCCCGCTTCTCAAAAACCTAAGGAGAAAGATGTAGTCATAGTTATCAGTGATGATGATGAGGAGGAAAAGCATGTATTGATGAACCTTAAATACCTTGCTTCGAGCTCCACTTCTAAAAAACCTGAGGGGGCAGATGTAGTCATAGGTATCAGTGACGAGGAGGAGGAAAAGATGGCTTTGCAGCCATCTTTTCCAAAGAAAACACTTCCAATGGCTGGGCAAACCAAGGAAAGACGGTTCCATAGAAAATGGACCAAAGAAGAAGAAGAGGCTTTAAGGCATGGTTATAAAAAGTATAAAACAAATTGGAAATTGATTTTGGGTGATCCAGCCTACAAAGCTTGCCATGGCAGAACTAATGTTGATTTGAAAGACAAATGGCGGAATTTGATGAGGGCTAATGACAGGGCTAAGGCGAAGGCCTGCTACTCATTACCAAGTACGAAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

776

Amino Acids

87.75

Weight (kDa)

6.8

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF2A PF08662 340 - 527 1e-31 Eukaryotic translation initiation factor eIF2A
Beta-prop_WDR19_1st PF23389 350 - 481 1.7e-06 WDR19 first beta-propeller
Beta-prop_THOC3 PF25174 391 - 499 2.4e-07 THOC3 beta-propeller domain
Myb_DNA-binding PF00249 708 - 756 5.5e-07 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 710 - 761 3.5e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019002)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0447321
rosa_laevigata RLG00000026012
rosa_multiflora Rmu_ssc0000396.1_g000008
rosa_roxburghii Rroxscaffold_5G00387700
rosa_samantha Rh4AG441000 Rh4BG450300 Rh4CG467400 Rh4DG448700
rosa_wichuraiana Rw4G037750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2166
Acc36I ACCTGC 1 cut(s) 976
Acc65I GGTACC 1 cut(s) 395
AccB1I GGYRCC 3 cut(s) 395, 536, 959
AccB7I CCANNNNNTGG 1 cut(s) 2093
AciI CCGC 5 cut(s) 44, 1264, 1549, 1881, 2260
AclWI GGATC 2 cut(s) 1581, 2198
AcoI YGGCCR 2 cut(s) 558, 1546
AcsI RAATTY 6 cut(s) 315, 451, 1152, 1390, 1397, 2263
AcuI CTGAAG 2 cut(s) 880, 1329
AcyI GRCGYC 1 cut(s) 764
AdeI CACNNNGTG 1 cut(s) 310
AfaI GTAC 7 cut(s) 150, 397, 446, 1052, 1099, 1865, 2320
AfiI CCNNNNNNNGG 5 cut(s) 390, 486, 1349, 1821, 2093
AgsI TTSAA 9 cut(s) 94, 100, 380, 430, 781, 989, 1375, 1405, 2248
AjnI CCWGG 3 cut(s) 399, 610, 1498
AjuI GAANNNNNNNTTGG 2 cut(s) 2180, 2212
AloI GAACNNNNNNTCC 2 cut(s) 2099, 2131
Alw21I GWGCWC 1 cut(s) 1985
Alw26I GTCTC 4 cut(s) 175, 180, 221, 240
AlwI GGATC 2 cut(s) 1581, 2198
Ama87I CYCGRG 1 cut(s) 1457
AoxI GGCC 7 cut(s) 558, 770, 1210, 1351, 1546, 1604, 2298
ApeKI GCWGC 3 cut(s) 674, 1271, 2059
ApoI RAATTY 6 cut(s) 315, 451, 1152, 1390, 1397, 2263
ArsI GACNNNNNNTTYG 2 cut(s) 794, 826
Asp700I GAANNNNTTC 3 cut(s) 317, 455, 1794
Asp718I GGTACC 1 cut(s) 395
AspS9I GGNCC 7 cut(s) 477, 731, 771, 786, 1210, 1638, 2130
AsuC2I CCSGG 1 cut(s) 1474
AsuHPI GGTGA 9 cut(s) 292, 296, 601, 626, 671, 897, 988, 1332, 2213
AvaI CYCGRG 1 cut(s) 1457
AvaII GGWCC 5 cut(s) 477, 731, 786, 1638, 2130
AxyI CCTNAGG 2 cut(s) 1896, 2001
BaeI ACNNNNGTAYC 2 cut(s) 387, 420
BalI TGGCCA 1 cut(s) 560
BanI GGYRCC 3 cut(s) 395, 536, 959
BanII GRGCYC 3 cut(s) 441, 1880, 1985
BarI GAAGNNNNNNTAC 2 cut(s) 2288, 2320
BauI CACGAG 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 1985
BbvI GCAGC 3 cut(s) 661, 1258, 2071
BccI CCATC 4 cut(s) 63, 1538, 2044, 2071
BceAI ACGGC 1 cut(s) 1467
BciT130I CCWGG 3 cut(s) 401, 612, 1500
BcnI CCSGG 1 cut(s) 1474
BcoDI GTCTC 4 cut(s) 175, 180, 221, 240
BfaI CTAG 1 cut(s) 1029
BfmI CTRYAG 1 cut(s) 634
BfuAI ACCTGC 1 cut(s) 976
BglII AGATCT 1 cut(s) 126
BisI GCNGC 4 cut(s) 675, 1272, 1549, 2060
BlsI GCNGC 4 cut(s) 676, 1273, 1550, 2061
Bme1390I CCNGG 4 cut(s) 401, 612, 1474, 1500
Bme18I GGWCC 5 cut(s) 477, 731, 786, 1638, 2130
BmeT110I CYCGRG 1 cut(s) 1457
BmgT120I GGNCC 7 cut(s) 477, 731, 771, 786, 1210, 1638, 2130
BmiI GGNNCC 9 cut(s) 397, 479, 538, 961, 1203, 1381, 1466, 1639, 2117
BmrFI CCNGG 4 cut(s) 401, 612, 1474, 1500
BmrI ACTGGG 3 cut(s) 768, 990, 1767
BmsI GCATC 6 cut(s) 580, 904, 940, 1149, 1258, 1801
BmuI ACTGGG 3 cut(s) 768, 990, 1767
Bpu10I CCTNAGC 1 cut(s) 2289
BpuEI CTTGAG 1 cut(s) 1844
BpuMI CCSGG 1 cut(s) 1474
Bsa29I ATCGAT 1 cut(s) 1143
BsaHI GRCGYC 1 cut(s) 764
BsaJI CCNNGG 7 cut(s) 399, 576, 1206, 1499, 1601, 2103, 2224
BsaWI WCCGGW 1 cut(s) 788
BsaXI ACNNNNNCTCC 4 cut(s) 1151, 1181, 1480, 1510
Bsc4I CCNNNNNNNGG 5 cut(s) 390, 486, 1349, 1821, 2093
Bse1I ACTGG 5 cut(s) 706, 774, 996, 1058, 1762
Bse21I CCTNAGG 2 cut(s) 1896, 2001
Bse3DI GCAATG 2 cut(s) 1069, 1292
BseBI CCWGG 3 cut(s) 401, 612, 1500
BseCI ATCGAT 1 cut(s) 1143
BseDI CCNNGG 7 cut(s) 399, 576, 1206, 1499, 1601, 2103, 2224
BseGI GGATG 9 cut(s) 36, 55, 192, 355, 571, 1273, 1417, 1816, 1834
BseLI CCNNNNNNNGG 5 cut(s) 390, 486, 1349, 1821, 2093
BseMI GCAATG 2 cut(s) 1069, 1292
BseMII CTCAG 3 cut(s) 426, 1321, 1992
BseNI ACTGG 5 cut(s) 706, 774, 996, 1058, 1762
BseRI GAGGAG 4 cut(s) 198, 1952, 2051, 2054
BseXI GCAGC 3 cut(s) 661, 1258, 2071
BseYI CCCAGC 1 cut(s) 2093
BsgI GTGCAG 1 cut(s) 440
BshFI GGCC 7 cut(s) 560, 772, 1212, 1353, 1548, 1606, 2300
BshNI GGYRCC 3 cut(s) 395, 536, 959
BshVI ATCGAT 1 cut(s) 1143
BsiHKAI GWGCWC 1 cut(s) 1985
BsiHKCI CYCGRG 1 cut(s) 1457
BsiSI CCGG 2 cut(s) 789, 1473
BslFI GGGAC 5 cut(s) 89, 463, 502, 1333, 1516
BslI CCNNNNNNNGG 5 cut(s) 390, 486, 1349, 1821, 2093
BsmAI GTCTC 4 cut(s) 175, 180, 221, 240
BsmBI CGTCTC 1 cut(s) 180
BsmFI GGGAC 5 cut(s) 89, 463, 502, 1333, 1516
BsmI GAATGC 1 cut(s) 1660
BsnI GGCC 7 cut(s) 560, 772, 1212, 1353, 1548, 1606, 2300
BsoBI CYCGRG 1 cut(s) 1457
Bsp1286I GDGCHC 3 cut(s) 441, 1880, 1985
Bsp1407I TGTACA 1 cut(s) 444
Bsp143I GATC 6 cut(s) 126, 301, 364, 1132, 1573, 2203
Bsp19I CCATGG 1 cut(s) 2224
BspACI CCGC 5 cut(s) 44, 1264, 1549, 1881, 2260
BspANI GGCC 7 cut(s) 560, 772, 1212, 1353, 1548, 1606, 2300
BspCNI CTCAG 3 cut(s) 427, 1320, 1993
BspDI ATCGAT 1 cut(s) 1143
BspHI TCATGA 2 cut(s) 736, 1510
BspLI GGNNCC 9 cut(s) 397, 479, 538, 961, 1203, 1381, 1466, 1639, 2117
BspMI ACCTGC 1 cut(s) 976
BspPI GGATC 2 cut(s) 1581, 2198
BspT107I GGYRCC 3 cut(s) 395, 536, 959
BsrDI GCAATG 2 cut(s) 1069, 1292
BsrGI TGTACA 1 cut(s) 444
BsrI ACTGG 5 cut(s) 706, 774, 996, 1058, 1762
BssECI CCNNGG 7 cut(s) 399, 576, 1206, 1499, 1601, 2103, 2224
BssMI GATC 6 cut(s) 126, 301, 364, 1132, 1573, 2203
BssNI GRCGYC 1 cut(s) 764
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 5 cut(s) 576, 1206, 1601, 2103, 2224
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 3 cut(s) 401, 612, 1500
Bst4CI ACNGT 7 cut(s) 638, 656, 877, 1097, 1167, 1426, 2115
Bst6I CTCTTC 3 cut(s) 790, 1583, 2139
BstACI GRCGYC 1 cut(s) 764
BstAUI TGTACA 1 cut(s) 444
BstC8I GCNNGC 5 cut(s) 298, 1276, 1658, 2221, 2302
BstDEI CTNAG 6 cut(s) 435, 1307, 1623, 1896, 2001, 2289
BstDSI CCRYGG 1 cut(s) 2224
BstEII GGTNACC 2 cut(s) 903, 1338
BstENI CCTNNNNNAGG 1 cut(s) 1819
BstF5I GGATG 9 cut(s) 36, 55, 192, 355, 571, 1273, 1417, 1816, 1834
BstKTI GATC 6 cut(s) 129, 304, 367, 1135, 1576, 2206
BstMAI GTCTC 4 cut(s) 175, 180, 221, 240
BstMBI GATC 6 cut(s) 126, 301, 364, 1132, 1573, 2203
BstMWI GCNNNNNNNGC 8 cut(s) 293, 923, 959, 968, 1603, 1625, 1653, 2216
BstNI CCWGG 3 cut(s) 401, 612, 1500
BstNSI RCATGY 1 cut(s) 1952
BstPI GGTNACC 2 cut(s) 903, 1338
BstSCI CCNGG 4 cut(s) 399, 610, 1472, 1498
BstSFI CTRYAG 1 cut(s) 634
BstV1I GCAGC 3 cut(s) 661, 1258, 2071
BstX2I RGATCY 2 cut(s) 126, 1573
BstYI RGATCY 2 cut(s) 126, 1573
Bsu15I ATCGAT 1 cut(s) 1143
Bsu36I CCTNAGG 2 cut(s) 1896, 2001
BsuRI GGCC 7 cut(s) 560, 772, 1212, 1353, 1548, 1606, 2300
BsuTUI ATCGAT 1 cut(s) 1143
BtgI CCRYGG 1 cut(s) 2224
BtsCI GGATG 9 cut(s) 36, 55, 192, 355, 571, 1273, 1417, 1816, 1834
BtsI GCAGTG 1 cut(s) 1185
BtsIMutI CAGTG 6 cut(s) 643, 661, 1185, 1664, 1930, 2035
BveI ACCTGC 1 cut(s) 976
Cac8I GCNNGC 5 cut(s) 298, 1276, 1658, 2221, 2302
CciI TCATGA 2 cut(s) 736, 1510
Cfr13I GGNCC 7 cut(s) 477, 731, 771, 786, 1210, 1638, 2130
ClaI ATCGAT 1 cut(s) 1143
CseI GACGC 1 cut(s) 753
Csp6I GTAC 7 cut(s) 149, 396, 445, 1051, 1098, 1864, 2319
CviQI GTAC 7 cut(s) 149, 396, 445, 1051, 1098, 1864, 2319
DdeI CTNAG 6 cut(s) 435, 1307, 1623, 1896, 2001, 2289
DpnI GATC 6 cut(s) 128, 303, 366, 1134, 1575, 2205
DpnII GATC 6 cut(s) 126, 301, 364, 1132, 1573, 2203
DraIII CACNNNGTG 1 cut(s) 310
EaeI YGGCCR 2 cut(s) 558, 1546
Eam1104I CTCTTC 3 cut(s) 790, 1583, 2139
EarI CTCTTC 3 cut(s) 790, 1583, 2139
EciI GGCGGA 1 cut(s) 2275
Ecl136II GAGCTC 1 cut(s) 1983
Eco130I CCWWGG 5 cut(s) 576, 1206, 1601, 2103, 2224
Eco147I AGGCCT 1 cut(s) 2300
Eco24I GRGCYC 3 cut(s) 441, 1880, 1985
Eco32I GATATC 1 cut(s) 520
Eco47I GGWCC 5 cut(s) 477, 731, 786, 1638, 2130
Eco53kI GAGCTC 1 cut(s) 1983
Eco57I CTGAAG 2 cut(s) 880, 1329
Eco81I CCTNAGG 2 cut(s) 1896, 2001
Eco88I CYCGRG 1 cut(s) 1457
Eco91I GGTNACC 2 cut(s) 903, 1338
EcoICRI GAGCTC 1 cut(s) 1983
EcoNI CCTNNNNNAGG 1 cut(s) 1819
EcoO109I RGGNCCY 1 cut(s) 731
EcoO65I GGTNACC 2 cut(s) 903, 1338
EcoRI GAATTC 1 cut(s) 451
EcoRII CCWGG 3 cut(s) 399, 610, 1498
EcoRV GATATC 1 cut(s) 520
EcoT14I CCWWGG 5 cut(s) 576, 1206, 1601, 2103, 2224
EcoT38I GRGCYC 3 cut(s) 441, 1880, 1985
ErhI CCWWGG 5 cut(s) 576, 1206, 1601, 2103, 2224
Esp3I CGTCTC 1 cut(s) 180
FalI AAGNNNNNCTT 4 cut(s) 116, 148, 1562, 1594
FaqI GGGAC 5 cut(s) 89, 463, 502, 1333, 1516
FauI CCCGC 3 cut(s) 51, 1257, 1888
Fnu4HI GCNGC 4 cut(s) 675, 1272, 1549, 2060
FokI GGATG 9 cut(s) 23, 42, 179, 362, 558, 1280, 1424, 1823, 1841
FriOI GRGCYC 3 cut(s) 441, 1880, 1985
Fsp4HI GCNGC 4 cut(s) 675, 1272, 1549, 2060
FspBI CTAG 1 cut(s) 1029
GluI GCNGC 4 cut(s) 675, 1272, 1549, 2060
GsaI CCCAGC 1 cut(s) 2097
HaeIII GGCC 7 cut(s) 560, 772, 1212, 1353, 1548, 1606, 2300
HapII CCGG 2 cut(s) 789, 1473
HgaI GACGC 1 cut(s) 753
Hin1I GRCGYC 1 cut(s) 764
HincII GTYRAC 1 cut(s) 1090
HindII GTYRAC 1 cut(s) 1090
HindIII AAGCTT 3 cut(s) 469, 1022, 2217
HinfI GANTC 4 cut(s) 269, 919, 941, 1303
HpaII CCGG 2 cut(s) 789, 1473
HphI GGTGA 9 cut(s) 292, 296, 601, 626, 671, 897, 988, 1332, 2213
Hpy166II GTNNAC 3 cut(s) 204, 445, 1090
Hpy188I TCNGA 9 cut(s) 9, 87, 228, 274, 436, 940, 1233, 1310, 1422
Hpy8I GTNNAC 3 cut(s) 204, 445, 1090
HpyAV CCTTC 4 cut(s) 277, 1353, 1564, 2290
HpyCH4III ACNGT 7 cut(s) 638, 656, 877, 1097, 1167, 1426, 2115
HpyCH4V TGCA 9 cut(s) 334, 421, 571, 677, 746, 1044, 1367, 1660, 2059
HpyF10VI GCNNNNNNNGC 8 cut(s) 293, 923, 959, 968, 1603, 1625, 1653, 2216
HpyF3I CTNAG 6 cut(s) 435, 1307, 1623, 1896, 2001, 2289
Hsp92I GRCGYC 1 cut(s) 764
KpnI GGTACC 1 cut(s) 399
Kzo9I GATC 6 cut(s) 126, 301, 364, 1132, 1573, 2203
LmnI GCTCC 3 cut(s) 293, 1159, 1988
Lsp1109I GCAGC 3 cut(s) 661, 1258, 2071
LweI GCATC 6 cut(s) 580, 904, 940, 1149, 1258, 1801
MaeI CTAG 1 cut(s) 1029
MaeIII GTNAC 6 cut(s) 188, 460, 903, 1183, 1338, 2030
MalI GATC 6 cut(s) 128, 303, 366, 1134, 1575, 2205
MboI GATC 6 cut(s) 126, 301, 364, 1132, 1573, 2203
MfeI CAATTG 2 cut(s) 21, 243
MflI RGATCY 2 cut(s) 126, 1573
MhlI GDGCHC 3 cut(s) 441, 1880, 1985
MlsI TGGCCA 1 cut(s) 560
MluNI TGGCCA 1 cut(s) 560
MlyI GAGTC 1 cut(s) 263
Mox20I TGGCCA 1 cut(s) 560
MroXI GAANNNNTTC 3 cut(s) 317, 455, 1794
MscI TGGCCA 1 cut(s) 560
MseI TTAA 5 cut(s) 507, 1128, 1527, 1965, 2153
Msp20I TGGCCA 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 866
MspI CCGG 2 cut(s) 789, 1473
MspR9I CCNGG 4 cut(s) 401, 612, 1474, 1500
MunI CAATTG 2 cut(s) 21, 243
Mva1269I GAATGC 1 cut(s) 1660
MvaI CCWGG 3 cut(s) 401, 612, 1500
MwoI GCNNNNNNNGC 8 cut(s) 293, 923, 959, 968, 1603, 1625, 1653, 2216
NciI CCSGG 1 cut(s) 1474
NcoI CCATGG 1 cut(s) 2224
NdeII GATC 6 cut(s) 126, 301, 364, 1132, 1573, 2203
NlaIV GGNNCC 9 cut(s) 397, 479, 538, 961, 1203, 1381, 1466, 1639, 2117
NmeAIII GCCGAG 1 cut(s) 1545
NmuCI GTSAC 5 cut(s) 460, 903, 1183, 1338, 2030
NspI RCATGY 1 cut(s) 1952
PaeR7I CTCGAG 1 cut(s) 1457
PagI TCATGA 2 cut(s) 736, 1510
PceI AGGCCT 1 cut(s) 2300
PctI GAATGC 1 cut(s) 1660
PdmI GAANNNNTTC 3 cut(s) 317, 455, 1794
PfeI GAWTC 3 cut(s) 919, 941, 1303
PflFI GACNNNGTC 1 cut(s) 1181
PflMI CCANNNNNTGG 1 cut(s) 2093
PkrI GCNGC 4 cut(s) 676, 1273, 1550, 2061
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
PpuMI RGGWCCY 1 cut(s) 731
PsiI TTATAA 1 cut(s) 2166
Psp124BI GAGCTC 1 cut(s) 1985
Psp5II RGGWCCY 1 cut(s) 731
Psp6I CCWGG 3 cut(s) 399, 610, 1498
PspEI GGTNACC 2 cut(s) 903, 1338
PspFI CCCAGC 1 cut(s) 2093
PspGI CCWGG 3 cut(s) 399, 610, 1498
PspN4I GGNNCC 9 cut(s) 397, 479, 538, 961, 1203, 1381, 1466, 1639, 2117
PspPI GGNCC 7 cut(s) 477, 731, 771, 786, 1210, 1638, 2130
PspPPI RGGWCCY 1 cut(s) 731
PsrI GAACNNNNNNTAC 2 cut(s) 1952, 1984
PsuI RGATCY 2 cut(s) 126, 1573
PsyI GACNNNGTC 1 cut(s) 1181
PvuII CAGCTG 1 cut(s) 866
RsaI GTAC 7 cut(s) 150, 397, 446, 1052, 1099, 1865, 2320
RsaNI GTAC 7 cut(s) 149, 396, 445, 1051, 1098, 1864, 2319
SacI GAGCTC 1 cut(s) 1985
SaqAI TTAA 5 cut(s) 507, 1128, 1527, 1965, 2153
SatI GCNGC 4 cut(s) 675, 1272, 1549, 2060
Sau3AI GATC 6 cut(s) 126, 301, 364, 1132, 1573, 2203
Sau96I GGNCC 7 cut(s) 477, 731, 771, 786, 1210, 1638, 2130
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 4 cut(s) 401, 612, 1474, 1500
SduI GDGCHC 3 cut(s) 441, 1880, 1985
SfaNI GCATC 6 cut(s) 580, 904, 940, 1149, 1258, 1801
SfcI CTRYAG 1 cut(s) 634
Sfr274I CTCGAG 1 cut(s) 1457
SinI GGWCC 5 cut(s) 477, 731, 786, 1638, 2130
SlaI CTCGAG 1 cut(s) 1457
SmlI CTYRAG 2 cut(s) 1457, 1859
SmoI CTYRAG 2 cut(s) 1457, 1859
SseBI AGGCCT 1 cut(s) 2300
SsiI CCGC 5 cut(s) 44, 1264, 1549, 1881, 2260
SspI AATATT 1 cut(s) 145
SspMI CTAG 1 cut(s) 1029
SstI GAGCTC 1 cut(s) 1985
StuI AGGCCT 1 cut(s) 2300
StyD4I CCNGG 4 cut(s) 399, 610, 1472, 1498
StyI CCWWGG 5 cut(s) 576, 1206, 1601, 2103, 2224
TaaI ACNGT 7 cut(s) 638, 656, 877, 1097, 1167, 1426, 2115
TaqI TCGA 4 cut(s) 1135, 1143, 1458, 1979
TaqII GACCGA 1 cut(s) 479
TatI WGTACW 3 cut(s) 444, 1050, 1097
TauI GCSGC 1 cut(s) 1551
TfiI GAWTC 3 cut(s) 919, 941, 1303
Tru1I TTAA 5 cut(s) 507, 1128, 1527, 1965, 2153
Tru9I TTAA 5 cut(s) 507, 1128, 1527, 1965, 2153
TscAI CASTG 6 cut(s) 643, 661, 1192, 1671, 1930, 2035
TseFI GTSAC 5 cut(s) 460, 903, 1183, 1338, 2030
TseI GCWGC 3 cut(s) 674, 1271, 2059
Tsp45I GTSAC 5 cut(s) 460, 903, 1183, 1338, 2030
TspDTI ATGAA 6 cut(s) 94, 1097, 1254, 1281, 1581, 1973
TspRI CASTG 6 cut(s) 643, 661, 1192, 1671, 1930, 2035
Tth111I GACNNNGTC 1 cut(s) 1181
Van91I CCANNNNNTGG 1 cut(s) 2093
VpaK11BI GGWCC 5 cut(s) 477, 731, 786, 1638, 2130
XagI CCTNNNNNAGG 1 cut(s) 1819
XapI RAATTY 6 cut(s) 315, 451, 1152, 1390, 1397, 2263
XceI RCATGY 1 cut(s) 1952
XcmI CCANNNNNNNNNTGG 2 cut(s) 970, 1390
XhoI CTCGAG 1 cut(s) 1457
XmnI GAANNNNTTC 3 cut(s) 317, 455, 1794
XspI CTAG 1 cut(s) 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.