Rw4G037750

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0019002
Physical Location & Seq
Reverse (-)
64226990 .. 64234270
7281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G037750.1

Sequence Viewer

Length: 2148 bp
ATGGTGAAGGCACAAGGAGCGAGCGATCACCTCGTGAAAATTTTCCCTGAAAATATGCAGGACATTCCACAGGATGAGAACTCCAAAGATCAGTTTCTAATTCAATCCAAGTCAGGGGGTACCCAGGTTTTATGGAATAATGTGCAGAAATTGAAATCTGAGCCCGTGTACAAGAATTCTTTCGTGACCGAAAGCTTATGGTCCCCTTTAGGGACTTATTTGGCAAATGTTAAGAAACAGGATATCATTCTTTTTAGAGGCACCTCTGCCTTACATCATGTGGCCACCTGTCTGCATCCCAAGGTTTTTGAGGTGAAATGGGTTGATTTTTCGCCTGGTGAGAATTATTTGGTGTCCTACAGTGTTGAACAAAAAAACACTGGTGAAATAACCAGAGCTGCAATAAGCATTTTTGATGTGAAAACTGGAAAAGTTGTGAAAAAAATTGTGGGAGGTCCTCATGATTTTGCAAATAAAGGAGTTGGGGCGTCTTGGCCCATTTTCAAATGGTCCGGTGGAAGAGATGACAAGTATTTTGCCCTAATCAGCAAGAATGTTATCTCTATTTATGAGATAAATACATTTACAGCCATATTGACGGTAGGGAATGTCATGGACTTCAGTTGGTCACCAACTGATGCGATTCTTGCTCTCTTTGTATCAGAATCGGTTGATGCTGGTGGTGCCATACCTGCTTTGGTGAAACTGGTTCAAATACCTAGTAAAGAGCAGTTGATTGAGAAGAAGCTTTCTAGTGTTAGTAAGTGCAAGATGTACTGGCAAAGCAATGGGGAGTATCTTGCTATGAAAGTTGACAGCAGTACAAACAATTTCGTTGAGCTTTTCCAAATTAAAGATCGAGGCATCGATAATAAAATTTTGGCGCTCCCGTATAATAAGGACAAGGTCACTGCTTTTGCTTGGGAACCCATGGGCCATAGGTTTGCTATTATTCAGAGTAATGAAGATATTAGTTTCTACTCCATGCGGGATGCTACCAGCACAGGCTGTGTTTCATTGCTCATGATTCTCAGAAGCAAGGGGACAAACTATCTCTTTTGGTCACCTTCTGGTGGCCTCATAATACTTGCAAGGTTGAATGGTTCCAATGGGAATTTGGAATTTTTCAATGTGGATGAACTTCGGACAGTTGCTATATCTAAACATATAGCAACGGCTCTTGAGTGGAACCCAACCGGGAGATATGTTGCTACTTCTACTCCTGGGACTTGTCATGAGAAGCCGAGTTTTAACATTTGGTCTTTTGATGGCCACCTACTCTACCAATTACAGAAGGATCTCTTCACAAAGTTTTCATGGCGACCAAGGCCACTCAACATGGCGACTAAGGCTATTATGGGGTCCATGAGCCATAACAGCTTGCATTCACTCAACATGGAAGATGATGAGTATTCACTCAACATGGAAGATGATGAGTATTCACTCAACATGGAAGATGATGATAATTCCATGAGTTTGACTGGGGGTATCAAGAAGTCTGTGGCTTTCATGGAAAAGTTTCACGAAGATTTGGATGCCTTTTATAGGCTGGATGATGAGGAAAGGCAGTTATTGATAGACCTCAAGCACCTCACTTTGAGCCCCGCTTCTCAAAAACCTAAGGAGAAAGATGTAGTCATAGTTATCAGTGATGATGAGGAGGAGGAAAAGCATGTATTGATGAACCTTAAATACCTTGCTTCGAGCTCCACTTCTAAAAAACCTGAGGGGGCAGATGTAGTCATAGGTATCAGTGACGAAGAGGAAAAGATGGCTTTGCAGCCATCTTCTCCAAAGAAAACACTTCCAATGGCTGGGCAAACCAAGGAAAGACGGTTCCATAGAAAATGGACCAAAGAAGAAGAAGAGGCTTTAAGGCATGGTTATAAAAAGTATAAAACAAATTGGAAATTGATTTTGGGTGATCCAGCCTACAAAGCTTGCCATGGCAGAACTAATGTTGATTTGAAAGACAAATGGCGGAATTTGATGAGGGCTGATGACAGGGCTAAGGCGAAGGCCTGCTACTCATTACCAATTCAAGGTCGAATTCTTGATATTGTTGGAGAAGTTGTAATTGATTTCATGTTTGCGGGTGGTTTCATGGACATTAACTGGAGTCCAAAAGCTACTTTCAGCACTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

715

Amino Acids

80.67

Weight (kDa)

6.52

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF2A PF08662 248 - 435 1.3e-31 Eukaryotic translation initiation factor eIF2A
Beta-prop_WDR19_1st PF23389 258 - 390 6.7e-06 WDR19 first beta-propeller
Beta-prop_THOC3 PF25174 277 - 407 5e-07 THOC3 beta-propeller domain
Myb_DNA-binding PF00249 615 - 663 5e-07 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 617 - 667 3.9e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019002)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0447321
rosa_laevigata RLG00000026012
rosa_multiflora Rmu_ssc0000396.1_g000008
rosa_roxburghii Rroxscaffold_5G00387700
rosa_samantha Rh4AG441000 Rh4BG450300 Rh4CG467400 Rh4DG448700
rosa_wichuraiana Rw4G037750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1887
Acc36I ACCTGC 1 cut(s) 700
Acc65I GGTACC 1 cut(s) 119
AccB1I GGYRCC 3 cut(s) 119, 260, 683
AccB7I CCANNNNNTGG 1 cut(s) 1814
AciI CCGC 4 cut(s) 988, 1605, 1981, 2093
AclWI GGATC 2 cut(s) 1305, 1919
AcoI YGGCCR 2 cut(s) 282, 1270
AcsI RAATTY 7 cut(s) 39, 175, 876, 1114, 1121, 1984, 2049
AcuI CTGAAG 1 cut(s) 604
AcyI GRCGYC 1 cut(s) 488
AdeI CACNNNGTG 1 cut(s) 34
AfaI GTAC 4 cut(s) 121, 170, 776, 823
AfiI CCNNNNNNNGG 6 cut(s) 114, 210, 1073, 1545, 1814, 2042
AgsI TTSAA 9 cut(s) 104, 154, 368, 505, 713, 1099, 1129, 1969, 2042
AjnI CCWGG 3 cut(s) 123, 334, 1222
AjuI GAANNNNNNNTTGG 2 cut(s) 1901, 1933
AloI GAACNNNNNNTCC 2 cut(s) 1820, 1852
AluBI AGCT 8 cut(s) 195, 398, 748, 841, 1380, 1707, 1940, 2129
AluI AGCT 8 cut(s) 195, 398, 748, 841, 1380, 1707, 1940, 2129
Alw21I GWGCWC 1 cut(s) 1709
AlwI GGATC 2 cut(s) 1305, 1919
AoxI GGCC 7 cut(s) 282, 494, 934, 1075, 1270, 1328, 2019
ApeKI GCWGC 2 cut(s) 398, 1780
ApoI RAATTY 7 cut(s) 39, 175, 876, 1114, 1121, 1984, 2049
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
Asp700I GAANNNNTTC 3 cut(s) 41, 179, 1518
Asp718I GGTACC 1 cut(s) 119
AspLEI GCGC 1 cut(s) 886
AspS9I GGNCC 7 cut(s) 201, 455, 495, 510, 934, 1362, 1851
AsuC2I CCSGG 1 cut(s) 1198
AsuHPI GGTGA 9 cut(s) 16, 20, 325, 350, 395, 621, 712, 1056, 1934
AvaII GGWCC 5 cut(s) 201, 455, 510, 1362, 1851
AxyI CCTNAGG 2 cut(s) 1620, 1725
BaeI ACNNNNGTAYC 2 cut(s) 111, 144
BalI TGGCCA 2 cut(s) 284, 1272
BanI GGYRCC 3 cut(s) 119, 260, 683
BanII GRGCYC 3 cut(s) 165, 1604, 1709
BarI GAAGNNNNNNTAC 2 cut(s) 2009, 2041
BauI CACGAG 1 cut(s) 32
Bbv12I GWGCWC 1 cut(s) 1709
BbvI GCAGC 2 cut(s) 385, 1792
BccI CCATC 3 cut(s) 1262, 1765, 1792
BceAI ACGGC 1 cut(s) 1191
BciT130I CCWGG 3 cut(s) 125, 336, 1224
BcnI CCSGG 1 cut(s) 1198
BfaI CTAG 2 cut(s) 720, 753
BfmI CTRYAG 1 cut(s) 358
BfoI RGCGCY 1 cut(s) 887
BfuAI ACCTGC 1 cut(s) 700
BisI GCNGC 2 cut(s) 399, 1781
BlsI GCNGC 2 cut(s) 400, 1782
Bme1390I CCNGG 4 cut(s) 125, 336, 1198, 1224
Bme18I GGWCC 5 cut(s) 201, 455, 510, 1362, 1851
BmgT120I GGNCC 7 cut(s) 201, 455, 495, 510, 934, 1362, 1851
BmiI GGNNCC 9 cut(s) 121, 203, 262, 685, 927, 1105, 1190, 1363, 1838
BmrFI CCNGG 4 cut(s) 125, 336, 1198, 1224
BmrI ACTGGG 1 cut(s) 1491
BmsI GCATC 6 cut(s) 304, 628, 664, 873, 982, 1525
BmuI ACTGGG 1 cut(s) 1491
BpmI CTGGAG 1 cut(s) 2137
Bpu10I CCTNAGC 1 cut(s) 2010
BpuEI CTTGAG 2 cut(s) 1202, 1568
BpuMI CCSGG 1 cut(s) 1198
Bsa29I ATCGAT 1 cut(s) 867
BsaHI GRCGYC 1 cut(s) 488
BsaJI CCNNGG 7 cut(s) 123, 300, 930, 1223, 1325, 1824, 1945
BsaWI WCCGGW 1 cut(s) 512
BsaXI ACNNNNNCTCC 4 cut(s) 1204, 1234, 2058, 2088
Bsc4I CCNNNNNNNGG 6 cut(s) 114, 210, 1073, 1545, 1814, 2042
Bse1I ACTGG 6 cut(s) 385, 430, 711, 782, 1486, 2120
Bse21I CCTNAGG 2 cut(s) 1620, 1725
Bse3DI GCAATG 2 cut(s) 793, 1016
BseBI CCWGG 3 cut(s) 125, 336, 1224
BseCI ATCGAT 1 cut(s) 867
BseDI CCNNGG 7 cut(s) 123, 300, 930, 1223, 1325, 1824, 1945
BseGI GGATG 6 cut(s) 79, 295, 997, 1141, 1540, 1558
BseLI CCNNNNNNNGG 6 cut(s) 114, 210, 1073, 1545, 1814, 2042
BseMI GCAATG 2 cut(s) 793, 1016
BseMII CTCAG 3 cut(s) 150, 1045, 1716
BseNI ACTGG 6 cut(s) 385, 430, 711, 782, 1486, 2120
BseRI GAGGAG 2 cut(s) 1673, 1676
BseXI GCAGC 2 cut(s) 385, 1792
BseYI CCCAGC 1 cut(s) 1814
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 7 cut(s) 284, 496, 936, 1077, 1272, 1330, 2021
BshNI GGYRCC 3 cut(s) 119, 260, 683
BshVI ATCGAT 1 cut(s) 867
BsiHKAI GWGCWC 1 cut(s) 1709
BsiSI CCGG 2 cut(s) 513, 1197
BslFI GGGAC 4 cut(s) 187, 226, 1057, 1240
BslI CCNNNNNNNGG 6 cut(s) 114, 210, 1073, 1545, 1814, 2042
BsmFI GGGAC 4 cut(s) 187, 226, 1057, 1240
BsmI GAATGC 1 cut(s) 1384
BsnI GGCC 7 cut(s) 284, 496, 936, 1077, 1272, 1330, 2021
Bsp1286I GDGCHC 3 cut(s) 165, 1604, 1709
Bsp1407I TGTACA 1 cut(s) 168
Bsp143I GATC 5 cut(s) 25, 88, 856, 1297, 1924
Bsp19I CCATGG 2 cut(s) 930, 1945
BspACI CCGC 4 cut(s) 988, 1605, 1981, 2093
BspANI GGCC 7 cut(s) 284, 496, 936, 1077, 1272, 1330, 2021
BspCNI CTCAG 3 cut(s) 151, 1044, 1717
BspDI ATCGAT 1 cut(s) 867
BspHI TCATGA 3 cut(s) 460, 1023, 1234
BspLI GGNNCC 9 cut(s) 121, 203, 262, 685, 927, 1105, 1190, 1363, 1838
BspMI ACCTGC 1 cut(s) 700
BspPI GGATC 2 cut(s) 1305, 1919
BspT107I GGYRCC 3 cut(s) 119, 260, 683
BsrDI GCAATG 2 cut(s) 793, 1016
BsrGI TGTACA 1 cut(s) 168
BsrI ACTGG 6 cut(s) 385, 430, 711, 782, 1486, 2120
BssECI CCNNGG 7 cut(s) 123, 300, 930, 1223, 1325, 1824, 1945
BssMI GATC 5 cut(s) 25, 88, 856, 1297, 1924
BssNI GRCGYC 1 cut(s) 488
BssSI CACGAG 1 cut(s) 32
BssT1I CCWWGG 5 cut(s) 300, 930, 1325, 1824, 1945
Bst2BI CACGAG 1 cut(s) 32
Bst2UI CCWGG 3 cut(s) 125, 336, 1224
Bst4CI ACNGT 4 cut(s) 362, 601, 1150, 1836
Bst6I CTCTTC 4 cut(s) 514, 1307, 1755, 1860
BstACI GRCGYC 1 cut(s) 488
BstAUI TGTACA 1 cut(s) 168
BstC8I GCNNGC 4 cut(s) 22, 1382, 1942, 2023
BstDEI CTNAG 6 cut(s) 159, 1031, 1347, 1620, 1725, 2010
BstDSI CCRYGG 2 cut(s) 930, 1945
BstEII GGTNACC 2 cut(s) 627, 1062
BstENI CCTNNNNNAGG 1 cut(s) 1543
BstF5I GGATG 6 cut(s) 79, 295, 997, 1141, 1540, 1558
BstH2I RGCGCY 1 cut(s) 887
BstHHI GCGC 1 cut(s) 886
BstKTI GATC 5 cut(s) 28, 91, 859, 1300, 1927
BstMBI GATC 5 cut(s) 25, 88, 856, 1297, 1924
BstMWI GCNNNNNNNGC 8 cut(s) 17, 647, 683, 692, 1327, 1349, 1377, 1937
BstNI CCWGG 3 cut(s) 125, 336, 1224
BstNSI RCATGY 1 cut(s) 1676
BstPI GGTNACC 2 cut(s) 627, 1062
BstSCI CCNGG 4 cut(s) 123, 334, 1196, 1222
BstSFI CTRYAG 1 cut(s) 358
BstV1I GCAGC 2 cut(s) 385, 1792
BstX2I RGATCY 1 cut(s) 1297
BstYI RGATCY 1 cut(s) 1297
Bsu15I ATCGAT 1 cut(s) 867
Bsu36I CCTNAGG 2 cut(s) 1620, 1725
BsuRI GGCC 7 cut(s) 284, 496, 936, 1077, 1272, 1330, 2021
BsuTUI ATCGAT 1 cut(s) 867
BtgI CCRYGG 2 cut(s) 930, 1945
BtsCI GGATG 6 cut(s) 79, 295, 997, 1141, 1540, 1558
BtsI GCAGTG 1 cut(s) 909
BtsIMutI CAGTG 5 cut(s) 367, 378, 909, 1654, 1759
BveI ACCTGC 1 cut(s) 700
Cac8I GCNNGC 4 cut(s) 22, 1382, 1942, 2023
CciI TCATGA 3 cut(s) 460, 1023, 1234
CfoI GCGC 1 cut(s) 886
Cfr13I GGNCC 7 cut(s) 201, 455, 495, 510, 934, 1362, 1851
ClaI ATCGAT 1 cut(s) 867
CseI GACGC 1 cut(s) 477
Csp6I GTAC 4 cut(s) 120, 169, 775, 822
CviQI GTAC 4 cut(s) 120, 169, 775, 822
DdeI CTNAG 6 cut(s) 159, 1031, 1347, 1620, 1725, 2010
DpnI GATC 5 cut(s) 27, 90, 858, 1299, 1926
DpnII GATC 5 cut(s) 25, 88, 856, 1297, 1924
DraIII CACNNNGTG 1 cut(s) 34
EaeI YGGCCR 2 cut(s) 282, 1270
Eam1104I CTCTTC 4 cut(s) 514, 1307, 1755, 1860
EarI CTCTTC 4 cut(s) 514, 1307, 1755, 1860
EciI GGCGGA 1 cut(s) 1996
Ecl136II GAGCTC 1 cut(s) 1707
Eco130I CCWWGG 5 cut(s) 300, 930, 1325, 1824, 1945
Eco147I AGGCCT 1 cut(s) 2021
Eco24I GRGCYC 3 cut(s) 165, 1604, 1709
Eco32I GATATC 1 cut(s) 244
Eco47I GGWCC 5 cut(s) 201, 455, 510, 1362, 1851
Eco53kI GAGCTC 1 cut(s) 1707
Eco57I CTGAAG 1 cut(s) 604
Eco81I CCTNAGG 2 cut(s) 1620, 1725
Eco91I GGTNACC 2 cut(s) 627, 1062
EcoICRI GAGCTC 1 cut(s) 1707
EcoNI CCTNNNNNAGG 1 cut(s) 1543
EcoO109I RGGNCCY 1 cut(s) 455
EcoO65I GGTNACC 2 cut(s) 627, 1062
EcoRI GAATTC 2 cut(s) 175, 2049
EcoRII CCWGG 3 cut(s) 123, 334, 1222
EcoRV GATATC 1 cut(s) 244
EcoT14I CCWWGG 5 cut(s) 300, 930, 1325, 1824, 1945
EcoT38I GRGCYC 3 cut(s) 165, 1604, 1709
ErhI CCWWGG 5 cut(s) 300, 930, 1325, 1824, 1945
FalI AAGNNNNNCTT 2 cut(s) 1286, 1318
FaqI GGGAC 4 cut(s) 187, 226, 1057, 1240
FauI CCCGC 3 cut(s) 981, 1612, 2086
Fnu4HI GCNGC 2 cut(s) 399, 1781
FokI GGATG 6 cut(s) 86, 282, 1004, 1148, 1547, 1565
FriOI GRGCYC 3 cut(s) 165, 1604, 1709
Fsp4HI GCNGC 2 cut(s) 399, 1781
FspBI CTAG 2 cut(s) 720, 753
GlaI GCGC 1 cut(s) 885
GluI GCNGC 2 cut(s) 399, 1781
GsaI CCCAGC 1 cut(s) 1818
GsuI CTGGAG 1 cut(s) 2137
HaeII RGCGCY 1 cut(s) 887
HaeIII GGCC 7 cut(s) 284, 496, 936, 1077, 1272, 1330, 2021
HapII CCGG 2 cut(s) 513, 1197
HgaI GACGC 1 cut(s) 477
HhaI GCGC 1 cut(s) 886
Hin1I GRCGYC 1 cut(s) 488
Hin6I GCGC 1 cut(s) 884
HinP1I GCGC 1 cut(s) 884
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HindIII AAGCTT 3 cut(s) 193, 746, 1938
HinfI GANTC 4 cut(s) 643, 665, 1027, 2119
HpaII CCGG 2 cut(s) 513, 1197
HphI GGTGA 9 cut(s) 16, 20, 325, 350, 395, 621, 712, 1056, 1934
Hpy166II GTNNAC 2 cut(s) 169, 814
Hpy188I TCNGA 5 cut(s) 160, 664, 957, 1034, 1146
Hpy8I GTNNAC 2 cut(s) 169, 814
HpyAV CCTTC 3 cut(s) 1077, 1288, 2011
HpyCH4III ACNGT 4 cut(s) 362, 601, 1150, 1836
HpyCH4V TGCA 9 cut(s) 58, 145, 295, 401, 470, 768, 1091, 1384, 1780
HpyF10VI GCNNNNNNNGC 8 cut(s) 17, 647, 683, 692, 1327, 1349, 1377, 1937
HpyF3I CTNAG 6 cut(s) 159, 1031, 1347, 1620, 1725, 2010
Hsp92I GRCGYC 1 cut(s) 488
HspAI GCGC 1 cut(s) 884
KpnI GGTACC 1 cut(s) 123
Kzo9I GATC 5 cut(s) 25, 88, 856, 1297, 1924
LmnI GCTCC 3 cut(s) 17, 891, 1712
Lsp1109I GCAGC 2 cut(s) 385, 1792
LweI GCATC 6 cut(s) 304, 628, 664, 873, 982, 1525
MaeI CTAG 2 cut(s) 720, 753
MaeIII GTNAC 5 cut(s) 184, 627, 907, 1062, 1754
MalI GATC 5 cut(s) 27, 90, 858, 1299, 1926
MboI GATC 5 cut(s) 25, 88, 856, 1297, 1924
MflI RGATCY 1 cut(s) 1297
MhlI GDGCHC 3 cut(s) 165, 1604, 1709
MlsI TGGCCA 2 cut(s) 284, 1272
MluNI TGGCCA 2 cut(s) 284, 1272
MlyI GAGTC 1 cut(s) 2128
MmeI TCCRAC 1 cut(s) 2044
Mox20I TGGCCA 2 cut(s) 284, 1272
MroXI GAANNNNTTC 3 cut(s) 41, 179, 1518
MscI TGGCCA 2 cut(s) 284, 1272
MseI TTAA 6 cut(s) 231, 852, 1251, 1689, 1874, 2112
Msp20I TGGCCA 2 cut(s) 284, 1272
MspI CCGG 2 cut(s) 513, 1197
MspR9I CCNGG 4 cut(s) 125, 336, 1198, 1224
Mva1269I GAATGC 1 cut(s) 1384
MvaI CCWGG 3 cut(s) 125, 336, 1224
MwoI GCNNNNNNNGC 8 cut(s) 17, 647, 683, 692, 1327, 1349, 1377, 1937
NciI CCSGG 1 cut(s) 1198
NcoI CCATGG 2 cut(s) 930, 1945
NdeII GATC 5 cut(s) 25, 88, 856, 1297, 1924
NlaIV GGNNCC 9 cut(s) 121, 203, 262, 685, 927, 1105, 1190, 1363, 1838
NmeAIII GCCGAG 1 cut(s) 1269
NmuCI GTSAC 5 cut(s) 184, 627, 907, 1062, 1754
NspI RCATGY 1 cut(s) 1676
PagI TCATGA 3 cut(s) 460, 1023, 1234
PceI AGGCCT 1 cut(s) 2021
PctI GAATGC 1 cut(s) 1384
PdmI GAANNNNTTC 3 cut(s) 41, 179, 1518
PfeI GAWTC 3 cut(s) 643, 665, 1027
PflFI GACNNNGTC 1 cut(s) 905
PflMI CCANNNNNTGG 1 cut(s) 1814
PkrI GCNGC 2 cut(s) 400, 1782
PleI GAGTC 1 cut(s) 2127
PpsI GAGTC 1 cut(s) 2127
PpuMI RGGWCCY 1 cut(s) 455
PsiI TTATAA 1 cut(s) 1887
Psp124BI GAGCTC 1 cut(s) 1709
Psp5II RGGWCCY 1 cut(s) 455
Psp6I CCWGG 3 cut(s) 123, 334, 1222
PspEI GGTNACC 2 cut(s) 627, 1062
PspFI CCCAGC 1 cut(s) 1814
PspGI CCWGG 3 cut(s) 123, 334, 1222
PspN4I GGNNCC 9 cut(s) 121, 203, 262, 685, 927, 1105, 1190, 1363, 1838
PspPI GGNCC 7 cut(s) 201, 455, 495, 510, 934, 1362, 1851
PspPPI RGGWCCY 1 cut(s) 455
PsrI GAACNNNNNNTAC 2 cut(s) 1676, 1708
PsuI RGATCY 1 cut(s) 1297
PsyI GACNNNGTC 1 cut(s) 905
RsaI GTAC 4 cut(s) 121, 170, 776, 823
RsaNI GTAC 4 cut(s) 120, 169, 775, 822
SacI GAGCTC 1 cut(s) 1709
SaqAI TTAA 6 cut(s) 231, 852, 1251, 1689, 1874, 2112
SatI GCNGC 2 cut(s) 399, 1781
Sau3AI GATC 5 cut(s) 25, 88, 856, 1297, 1924
Sau96I GGNCC 7 cut(s) 201, 455, 495, 510, 934, 1362, 1851
SchI GAGTC 1 cut(s) 2128
ScrFI CCNGG 4 cut(s) 125, 336, 1198, 1224
SduI GDGCHC 3 cut(s) 165, 1604, 1709
SfaNI GCATC 6 cut(s) 304, 628, 664, 873, 982, 1525
SfcI CTRYAG 1 cut(s) 358
SinI GGWCC 5 cut(s) 201, 455, 510, 1362, 1851
SmlI CTYRAG 2 cut(s) 1181, 1583
SmoI CTYRAG 2 cut(s) 1181, 1583
SseBI AGGCCT 1 cut(s) 2021
SsiI CCGC 4 cut(s) 988, 1605, 1981, 2093
SspMI CTAG 2 cut(s) 720, 753
SstI GAGCTC 1 cut(s) 1709
StuI AGGCCT 1 cut(s) 2021
StyD4I CCNGG 4 cut(s) 123, 334, 1196, 1222
StyI CCWWGG 5 cut(s) 300, 930, 1325, 1824, 1945
TaaI ACNGT 4 cut(s) 362, 601, 1150, 1836
TaqI TCGA 4 cut(s) 859, 867, 1703, 2047
TaqII GACCGA 1 cut(s) 203
TatI WGTACW 3 cut(s) 168, 774, 821
TfiI GAWTC 3 cut(s) 643, 665, 1027
Tru1I TTAA 6 cut(s) 231, 852, 1251, 1689, 1874, 2112
Tru9I TTAA 6 cut(s) 231, 852, 1251, 1689, 1874, 2112
TscAI CASTG 5 cut(s) 367, 385, 916, 1654, 1759
TseFI GTSAC 5 cut(s) 184, 627, 907, 1062, 1754
TseI GCWGC 2 cut(s) 398, 1780
Tsp45I GTSAC 5 cut(s) 184, 627, 907, 1062, 1754
TspDTI ATGAA 9 cut(s) 821, 978, 1005, 1152, 1305, 1498, 1697, 2074, 2092
TspRI CASTG 5 cut(s) 367, 385, 916, 1654, 1759
Tth111I GACNNNGTC 1 cut(s) 905
Van91I CCANNNNNTGG 1 cut(s) 1814
VpaK11BI GGWCC 5 cut(s) 201, 455, 510, 1362, 1851
XagI CCTNNNNNAGG 1 cut(s) 1543
XapI RAATTY 7 cut(s) 39, 175, 876, 1114, 1121, 1984, 2049
XceI RCATGY 1 cut(s) 1676
XcmI CCANNNNNNNNNTGG 2 cut(s) 694, 1114
XmnI GAANNNNTTC 3 cut(s) 41, 179, 1518
XspI CTAG 2 cut(s) 720, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.