RchiOBHm_Chr3g0452191

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0017098
Physical Location & Seq
Reverse (-)
2935078 .. 2937876
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1278 bp
ATGGCCCCCGACCAAGACGACGCCGTTCTCCCCGAGGGCGACCTCTCCTCCTCCTCCCAAGACGACGAGGAAGAAGACGTTGACGTTGACGAGGACGAGGACGACGACGTCGACGACGACGACGACGTCATCAACTCCGCCTCTCCTTCCTCCACCTCCAATTCCCTCCCCGTCGCCATAGCCACCGCTGCTCCCACCGTCACCGTCGCGCTCCCGGCTCCGCACGCCACCACCGCTCCGGCGACGACTACAACGACGGCCGTGCTTTCCGAGGGTTTGGATCCGAAGCGGCCGCGGACGATTCAGTACGACCCGGTCTTGCTGCCGCTGGCGGCGCCGGAGGAGAAGAAGCCGGCGGCGGCGCTGGACGACTCGAGGAGGCTGTTCCAGCGGCTGTGGACGGACGAGGACGAGATCGAGCTGCTGCAAGGGTTTCTGGACTACACGACGCAGAGAGGGAACAAAGGCGGGTCGCACCAAAACGACACCGCTTTGTTCTACGATCAGATCAAGTCGAAGCTCCAGCTGGAATTCAACAAGAACCAGCTGGTGGAGAAGCTCCGGCGCCTGAAGAAGAAGTACCGGAATGTTCTCAACAAAATCGCCAGCGGCAAAGAGGTCAGCTTCAAGAGCCCTCACGATCAGGCCACCTTCGAGATCTCCAGGAAGATATGGAGCAACATCGGCCGTATCGGAGTTGCTGGCGCCGACGACAACGACGACGAAGACCCTAACCCTAACCCCAACAATCCCAATTTCAACCATTTCGAGATCAAGACGGAAGAGGCCGCCGGATTTCAGAAGTCCACGCCGAAATCGCGGAAGCGGCAGCGGACGCCGGCGCAGCCGAAAACGCCGAGTTTCGTCGAGCCGGCGGCGGTGCCGGTGATCAAAGAGAATCATCACAATGTGAACAATGGCGGTGGCGTTGGTGGTGGTGGTGGTGGTGGGAGTAATTGTGGAAATTGTAATGTTCAGGGGATGATTGAGGAGACGGTGAAGAGTTGTTTGTCTCCTGTGTTGAGGGAGTTGATGAGCAGTTCAATGGGTGGAGGAGGGCCGTCTAGAGGGTTTGGAGGGCTGTCGTTGAATCCGATTCCGCTGAGCTTTGGCGGTCCTTCGATGAGTTTGAATTTTGGGGGTGGGGAAGCGGCGGATGAGCAGTGGAGGAAGCAGCAGATATTGGAGTTGGAGGTGTATTCGAAGCGATTGGAGTTGGTGCAGAATCAGATAAAAGTGGCATTGGATGAGTTGCGGTCCACAGGAGGAGGAGGCTGA

Protein Analysis

425

Amino Acids

45.79

Weight (kDa)

4.75

Isoelectric Point (pI)

58.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 129 - 226 2.9e-32 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017098)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20613
rosa_chinensis RchiOBHm_Chr3g0452191
rosa_laevigata RLG00000013009 RLG00000025619
rosa_multiflora Rmu_sc0021298.1_g000001
rosa_roxburghii Rroxscaffold_164G00435970 Rroxscaffold_6G00429330
rosa_rugosa Rorug02G0639100
rosa_samantha Rh3AG040900 Rh3BG042500 Rh3CG041000 Rh3DG041900
rosa_wichuraiana Rw3G003130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 107, 125
AatII GACGTC 2 cut(s) 111, 129
AccB1I GGYRCC 4 cut(s) 334, 564, 704, 880
AccB7I CCANNNNNTGG 1 cut(s) 550
AccBSI CCGCTC 1 cut(s) 236
AccI GTMKAC 1 cut(s) 111
AccII CGCG 3 cut(s) 209, 295, 820
AclWI GGATC 2 cut(s) 275, 288
AcoI YGGCCR 3 cut(s) 258, 290, 685
AcsI RAATTY 2 cut(s) 530, 1132
AcuI CTGAAG 1 cut(s) 590
AcyI GRCGYC 7 cut(s) 21, 108, 126, 335, 565, 705, 836
AdeI CACNNNGTG 1 cut(s) 910
AfaI GTAC 2 cut(s) 308, 581
AfiI CCNNNNNNNGG 2 cut(s) 550, 1067
AgsI TTSAA 6 cut(s) 535, 628, 760, 1044, 1090, 1132
AjnI CCWGG 1 cut(s) 662
AluBI AGCT 7 cut(s) 421, 520, 526, 547, 559, 624, 1107
AluI AGCT 7 cut(s) 421, 520, 526, 547, 559, 624, 1107
Alw26I GTCTC 2 cut(s) 986, 1017
AlwI GGATC 2 cut(s) 275, 288
AlwNI CAGNNNCTG 1 cut(s) 394
Ama87I CYCGRG 2 cut(s) 32, 373
AoxI GGCC 7 cut(s) 3, 258, 290, 645, 685, 786, 1058
ApeKI GCWGC 7 cut(s) 188, 322, 421, 424, 829, 844, 1174
ApoI RAATTY 2 cut(s) 530, 1132
AspLEI GCGC 6 cut(s) 211, 337, 364, 567, 707, 844
AspS9I GGNCC 4 cut(s) 4, 1058, 1115, 1257
AsuC2I CCSGG 2 cut(s) 215, 314
AsuHPI GGTGA 3 cut(s) 193, 898, 1009
AsuII TTCGAA 1 cut(s) 1202
AvaI CYCGRG 2 cut(s) 32, 373
AvaII GGWCC 2 cut(s) 1115, 1257
BamHI GGATCC 1 cut(s) 280
BanI GGYRCC 4 cut(s) 334, 564, 704, 880
BanII GRGCYC 1 cut(s) 635
BarI GAAGNNNNNNTAC 2 cut(s) 563, 595
BbsI GAAGAC 2 cut(s) 81, 732
BbvI GCAGC 7 cut(s) 175, 309, 408, 411, 841, 856, 1186
BceAI ACGGC 5 cut(s) 8, 245, 273, 672, 1045
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BciT130I CCWGG 1 cut(s) 664
BclI TGATCA 1 cut(s) 888
BcnI CCSGG 2 cut(s) 215, 314
BcoDI GTCTC 2 cut(s) 986, 1017
BfaI CTAG 1 cut(s) 1065
BfoI RGCGCY 4 cut(s) 338, 365, 568, 708
BglII AGATCT 1 cut(s) 657
BlpI GCTNAGC 1 cut(s) 1103
Bme1390I CCNGG 3 cut(s) 215, 314, 664
Bme18I GGWCC 2 cut(s) 1115, 1257
BmeT110I CYCGRG 2 cut(s) 32, 373
BmgT120I GGNCC 4 cut(s) 4, 1058, 1115, 1257
BmiI GGNNCC 7 cut(s) 6, 219, 282, 336, 566, 706, 882
BmrFI CCNGG 3 cut(s) 215, 314, 664
BpiI GAAGAC 2 cut(s) 81, 732
BpmI CTGGAG 2 cut(s) 506, 646
Bpu1102I GCTNAGC 1 cut(s) 1103
Bpu14I TTCGAA 1 cut(s) 1202
BpuMI CCSGG 2 cut(s) 215, 314
BsaHI GRCGYC 7 cut(s) 21, 108, 126, 335, 565, 705, 836
BsaJI CCNNGG 3 cut(s) 33, 270, 293
BsaWI WCCGGW 1 cut(s) 582
BsaXI ACNNNNNCTCC 8 cut(s) 32, 62, 175, 205, 220, 250, 943, 973
Bsc4I CCNNNNNNNGG 2 cut(s) 550, 1067
Bse118I RCCGGY 4 cut(s) 352, 838, 871, 883
BseBI CCWGG 1 cut(s) 664
BseDI CCNNGG 3 cut(s) 33, 270, 293
BseGI GGATG 3 cut(s) 987, 1162, 1252
BseLI CCNNNNNNNGG 2 cut(s) 550, 1067
BseMII CTCAG 1 cut(s) 1094
BseRI GAGGAG 7 cut(s) 37, 40, 43, 356, 391, 1004, 1068
BseX3I CGGCCG 3 cut(s) 258, 290, 685
BseXI GCAGC 7 cut(s) 175, 309, 408, 411, 841, 856, 1186
BsgI GTGCAG 1 cut(s) 1241
Bsh1236I CGCG 3 cut(s) 209, 295, 820
Bsh1285I CGRYCG 3 cut(s) 261, 293, 688
BshFI GGCC 7 cut(s) 5, 260, 292, 647, 687, 788, 1060
BshNI GGYRCC 4 cut(s) 334, 564, 704, 880
BsiEI CGRYCG 3 cut(s) 261, 293, 688
BsiHKCI CYCGRG 2 cut(s) 32, 373
BslI CCNNNNNNNGG 2 cut(s) 550, 1067
BsmAI GTCTC 2 cut(s) 986, 1017
BsmBI CGTCTC 1 cut(s) 986
BsnI GGCC 7 cut(s) 5, 260, 292, 647, 687, 788, 1060
BsoBI CYCGRG 2 cut(s) 32, 373
Bsp119I TTCGAA 1 cut(s) 1202
Bsp1286I GDGCHC 1 cut(s) 635
Bsp143I GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 888
Bsp1720I GCTNAGC 1 cut(s) 1103
BspANI GGCC 7 cut(s) 5, 260, 292, 647, 687, 788, 1060
BspCNI CTCAG 1 cut(s) 1095
BspFNI CGCG 3 cut(s) 209, 295, 820
BspLI GGNNCC 7 cut(s) 6, 219, 282, 336, 566, 706, 882
BspPI GGATC 2 cut(s) 275, 288
BspT104I TTCGAA 1 cut(s) 1202
BspT107I GGYRCC 4 cut(s) 334, 564, 704, 880
BsrBI CCGCTC 1 cut(s) 236
BsrFI RCCGGY 4 cut(s) 352, 838, 871, 883
BssAI RCCGGY 4 cut(s) 352, 838, 871, 883
BssECI CCNNGG 3 cut(s) 33, 270, 293
BssMI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 888
BssNI GRCGYC 7 cut(s) 21, 108, 126, 335, 565, 705, 836
Bst2UI CCWGG 1 cut(s) 664
Bst4CI ACNGT 3 cut(s) 199, 205, 997
Bst6I CTCTTC 2 cut(s) 777, 995
BstACI GRCGYC 7 cut(s) 21, 108, 126, 335, 565, 705, 836
BstBI TTCGAA 1 cut(s) 1202
BstC8I GCNNGC 7 cut(s) 225, 330, 354, 607, 703, 840, 873
BstDEI CTNAG 1 cut(s) 1103
BstDSI CCRYGG 1 cut(s) 293
BstF5I GGATG 3 cut(s) 987, 1162, 1252
BstFNI CGCG 3 cut(s) 209, 295, 820
BstH2I RGCGCY 4 cut(s) 338, 365, 568, 708
BstHHI GCGC 6 cut(s) 211, 337, 364, 567, 707, 844
BstKTI GATC 8 cut(s) 283, 417, 505, 510, 643, 660, 774, 891
BstMAI GTCTC 2 cut(s) 986, 1017
BstMBI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 888
BstMCI CGRYCG 3 cut(s) 261, 293, 688
BstNI CCWGG 1 cut(s) 664
BstSCI CCNGG 3 cut(s) 213, 312, 662
BstUI CGCG 3 cut(s) 209, 295, 820
BstV1I GCAGC 7 cut(s) 175, 309, 408, 411, 841, 856, 1186
BstV2I GAAGAC 2 cut(s) 81, 732
BstX2I RGATCY 2 cut(s) 280, 657
BstYI RGATCY 2 cut(s) 280, 657
BstZI CGGCCG 3 cut(s) 258, 290, 685
BsuRI GGCC 7 cut(s) 5, 260, 292, 647, 687, 788, 1060
BtgI CCRYGG 1 cut(s) 293
BtsCI GGATG 3 cut(s) 987, 1162, 1252
BtsI GCAGTG 1 cut(s) 1169
BtsIMutI CAGTG 1 cut(s) 1169
Cac8I GCNNGC 7 cut(s) 225, 330, 354, 607, 703, 840, 873
CaiI CAGNNNCTG 1 cut(s) 394
CciNI GCGGCCGC 1 cut(s) 290
CfoI GCGC 6 cut(s) 211, 337, 364, 567, 707, 844
Cfr10I RCCGGY 4 cut(s) 352, 838, 871, 883
Cfr13I GGNCC 4 cut(s) 4, 1058, 1115, 1257
Cfr42I CCGCGG 1 cut(s) 296
CseI GACGC 3 cut(s) 29, 457, 844
Csp6I GTAC 2 cut(s) 307, 580
CspCI CAANNNNNGTGG 2 cut(s) 904, 939
CviQI GTAC 2 cut(s) 307, 580
DdeI CTNAG 1 cut(s) 1103
DinI GGCGCC 3 cut(s) 336, 566, 706
DpnI GATC 8 cut(s) 282, 416, 504, 509, 642, 659, 773, 890
DpnII GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 888
DraIII CACNNNGTG 1 cut(s) 910
DrdI GACNNNNNNGTC 2 cut(s) 107, 125
DseDI GACNNNNNNGTC 2 cut(s) 107, 125
EaeI YGGCCR 3 cut(s) 258, 290, 685
EagI CGGCCG 3 cut(s) 258, 290, 685
Eam1104I CTCTTC 2 cut(s) 777, 995
EarI CTCTTC 2 cut(s) 777, 995
EciI GGCGGA 2 cut(s) 127, 1169
EclXI CGGCCG 3 cut(s) 258, 290, 685
Eco24I GRGCYC 1 cut(s) 635
Eco47I GGWCC 2 cut(s) 1115, 1257
Eco52I CGGCCG 3 cut(s) 258, 290, 685
Eco57I CTGAAG 1 cut(s) 590
Eco88I CYCGRG 2 cut(s) 32, 373
EcoRI GAATTC 1 cut(s) 530
EcoRII CCWGG 1 cut(s) 662
EcoT38I GRGCYC 1 cut(s) 635
EgeI GGCGCC 3 cut(s) 336, 566, 706
EheI GGCGCC 3 cut(s) 336, 566, 706
Esp3I CGTCTC 1 cut(s) 986
FaiI YATR 2 cut(s) 179, 673
FauI CCCGC 1 cut(s) 461
FbaI TGATCA 1 cut(s) 888
FblI GTMKAC 1 cut(s) 111
FokI GGATG 3 cut(s) 994, 1169, 1259
FriOI GRGCYC 1 cut(s) 635
FspBI CTAG 1 cut(s) 1065
GlaI GCGC 6 cut(s) 210, 336, 363, 566, 706, 843
GsuI CTGGAG 2 cut(s) 506, 646
HaeII RGCGCY 4 cut(s) 338, 365, 568, 708
HaeIII GGCC 7 cut(s) 5, 260, 292, 647, 687, 788, 1060
HgaI GACGC 3 cut(s) 29, 457, 844
HhaI GCGC 6 cut(s) 211, 337, 364, 567, 707, 844
Hin1I GRCGYC 7 cut(s) 21, 108, 126, 335, 565, 705, 836
Hin6I GCGC 6 cut(s) 209, 335, 362, 565, 705, 842
HinP1I GCGC 6 cut(s) 209, 335, 362, 565, 705, 842
HincII GTYRAC 3 cut(s) 82, 88, 112
HindII GTYRAC 3 cut(s) 82, 88, 112
HinfI GANTC 6 cut(s) 301, 371, 898, 1090, 1096, 1225
HphI GGTGA 3 cut(s) 193, 898, 1009
Hpy166II GTNNAC 7 cut(s) 82, 88, 112, 399, 807, 913, 1260
Hpy188I TCNGA 7 cut(s) 271, 285, 507, 695, 801, 1095, 1230
Hpy188III TCNNGA 7 cut(s) 437, 628, 638, 655, 769, 775, 1065
Hpy8I GTNNAC 7 cut(s) 82, 88, 112, 399, 807, 913, 1260
HpyAV CCTTC 3 cut(s) 156, 661, 1128
HpyCH4III ACNGT 3 cut(s) 199, 205, 997
HpyCH4IV ACGT 4 cut(s) 78, 84, 108, 126
HpyCH4V TGCA 2 cut(s) 427, 1222
HpyF3I CTNAG 1 cut(s) 1103
HpySE526I ACGT 4 cut(s) 78, 84, 108, 126
Hsp92I GRCGYC 7 cut(s) 21, 108, 126, 335, 565, 705, 836
HspAI GCGC 6 cut(s) 209, 335, 362, 565, 705, 842
KasI GGCGCC 3 cut(s) 334, 564, 704
KroI GCCGGC 3 cut(s) 352, 838, 871
KroNI GCCGGC 3 cut(s) 354, 840, 873
Ksp22I TGATCA 1 cut(s) 888
KspI CCGCGG 1 cut(s) 296
Kzo9I GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 888
LmnI GCTCC 7 cut(s) 196, 216, 223, 241, 525, 564, 675
Lsp1109I GCAGC 7 cut(s) 175, 309, 408, 411, 841, 856, 1186
MaeI CTAG 1 cut(s) 1065
MaeII ACGT 4 cut(s) 78, 84, 108, 126
MaeIII GTNAC 1 cut(s) 199
MalI GATC 8 cut(s) 282, 416, 504, 509, 642, 659, 773, 890
MbiI CCGCTC 1 cut(s) 236
MboI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 888
MboII GAAGA 9 cut(s) 83, 86, 358, 583, 586, 679, 737, 794, 1012
MflI RGATCY 2 cut(s) 280, 657
MhlI GDGCHC 1 cut(s) 635
MluCI AATT 6 cut(s) 160, 530, 754, 955, 964, 1132
Mly113I GGCGCC 3 cut(s) 335, 565, 705
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 1170
MreI CGCCGGCG 1 cut(s) 838
MroNI GCCGGC 3 cut(s) 352, 838, 871
MslI CAYNNNNRTG 1 cut(s) 906
MspA1I CMGCKG 9 cut(s) 188, 295, 328, 391, 526, 547, 609, 832, 1102
MspR9I CCNGG 3 cut(s) 215, 314, 664
MvaI CCWGG 1 cut(s) 664
MvnI CGCG 3 cut(s) 209, 295, 820
NaeI GCCGGC 3 cut(s) 354, 840, 873
NarI GGCGCC 3 cut(s) 335, 565, 705
NciI CCSGG 2 cut(s) 215, 314
NdeII GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 888
NgoMIV GCCGGC 3 cut(s) 352, 838, 871
NlaIV GGNNCC 7 cut(s) 6, 219, 282, 336, 566, 706, 882
NmeAIII GCCGAG 1 cut(s) 882
NmuCI GTSAC 1 cut(s) 199
NotI GCGGCCGC 1 cut(s) 290
NspV TTCGAA 1 cut(s) 1202
PaeR7I CTCGAG 1 cut(s) 373
PdiI GCCGGC 3 cut(s) 354, 840, 873
PfeI GAWTC 5 cut(s) 301, 898, 1090, 1096, 1225
PflFI GACNNNGTC 3 cut(s) 107, 125, 314
PflMI CCANNNNNTGG 1 cut(s) 550
PfoI TCCNGGA 1 cut(s) 662
PleI GAGTC 1 cut(s) 365
PluTI GGCGCC 3 cut(s) 338, 568, 708
PpsI GAGTC 1 cut(s) 365
Psp6I CCWGG 1 cut(s) 662
PspGI CCWGG 1 cut(s) 662
PspN4I GGNNCC 7 cut(s) 6, 219, 282, 336, 566, 706, 882
PspPI GGNCC 4 cut(s) 4, 1058, 1115, 1257
PspXI VCTCGAGB 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 394
PsuI RGATCY 2 cut(s) 280, 657
PsyI GACNNNGTC 3 cut(s) 107, 125, 314
PvuII CAGCTG 2 cut(s) 526, 547
RsaI GTAC 2 cut(s) 308, 581
RsaNI GTAC 2 cut(s) 307, 580
RseI CAYNNNNRTG 1 cut(s) 906
SacII CCGCGG 1 cut(s) 296
SalI GTCGAC 1 cut(s) 110
Sau3AI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 888
Sau96I GGNCC 4 cut(s) 4, 1058, 1115, 1257
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 3 cut(s) 215, 314, 664
SduI GDGCHC 1 cut(s) 635
SfoI GGCGCC 3 cut(s) 336, 566, 706
Sfr274I CTCGAG 1 cut(s) 373
Sfr303I CCGCGG 1 cut(s) 296
SfuI TTCGAA 1 cut(s) 1202
SgrAI CRCCGGYG 1 cut(s) 838
SgrBI CCGCGG 1 cut(s) 296
SgrDI CGTCGACG 1 cut(s) 110
SinI GGWCC 2 cut(s) 1115, 1257
SlaI CTCGAG 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 906
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 6 cut(s) 160, 530, 754, 955, 964, 1132
SspDI GGCGCC 3 cut(s) 334, 564, 704
SspMI CTAG 1 cut(s) 1065
StyD4I CCNGG 3 cut(s) 213, 312, 662
TaaI ACNGT 3 cut(s) 199, 205, 997
TaiI ACGT 4 cut(s) 81, 87, 111, 129
TaqI TCGA 9 cut(s) 111, 374, 417, 515, 654, 768, 867, 1121, 1202
TasI AATT 6 cut(s) 160, 530, 754, 955, 964, 1132
TfiI GAWTC 5 cut(s) 301, 898, 1090, 1096, 1225
TscAI CASTG 1 cut(s) 1169
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 7 cut(s) 188, 322, 421, 424, 829, 844, 1174
Tsp45I GTSAC 1 cut(s) 199
TspGWI ACGGA 2 cut(s) 416, 794
TspRI CASTG 1 cut(s) 1169
Tth111I GACNNNGTC 3 cut(s) 107, 125, 314
Van91I CCANNNNNTGG 1 cut(s) 550
VpaK11BI GGWCC 2 cut(s) 1115, 1257
XapI RAATTY 2 cut(s) 530, 1132
XbaI TCTAGA 1 cut(s) 1064
XhoI CTCGAG 1 cut(s) 373
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 1 cut(s) 1065
ZraI GACGTC 2 cut(s) 109, 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.